AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008708

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008708.t1 MTR_4g088030 96.375 331 12 0 1 331 22 352 0.0 669
MS.gene008708.t1 MTR_4g087730 86.544 327 44 0 5 331 38 364 0.0 596
MS.gene008708.t1 MTR_2g015720 76.161 323 76 1 9 331 39 360 0.0 525
MS.gene008708.t1 MTR_2g091175 60.062 323 126 2 9 330 47 367 1.84e-147 420
MS.gene008708.t1 MTR_4g079700 58.567 321 132 1 9 329 44 363 1.08e-140 402
MS.gene008708.t1 MTR_3g049330 56.037 323 140 2 10 330 29 351 6.63e-136 390
MS.gene008708.t1 MTR_2g073510 55.452 321 140 3 9 326 30 350 2.46e-130 376
MS.gene008708.t1 MTR_3g021060 52.174 322 152 2 9 329 43 363 8.00e-127 367
MS.gene008708.t1 MTR_3g035830 51.875 320 152 2 11 329 30 348 6.55e-126 364
MS.gene008708.t1 MTR_3g021090 51.863 322 153 2 5 325 24 344 6.68e-125 364
MS.gene008708.t1 MTR_5g034810 53.086 324 148 3 9 330 32 353 1.17e-124 362
MS.gene008708.t1 MTR_5g071350 49.300 357 153 5 2 330 44 400 4.45e-119 349
MS.gene008708.t1 MTR_3g464570 49.275 345 159 4 2 330 33 377 6.73e-119 348
MS.gene008708.t1 MTR_2g073500 47.561 328 153 7 12 330 29 346 1.73e-107 318
MS.gene008708.t1 MTR_1g111010 46.483 327 157 9 9 330 29 342 6.22e-99 296
MS.gene008708.t1 MTR_0136s0010 39.359 343 184 6 12 331 170 511 2.43e-91 282
MS.gene008708.t1 MTR_7g093450 40.525 343 180 6 12 331 248 589 1.78e-89 280
MS.gene008708.t1 MTR_1g050448 40.361 332 181 5 9 329 50 375 3.07e-88 270
MS.gene008708.t1 MTR_3g464570 50.000 270 119 4 77 330 4 273 6.63e-88 265
MS.gene008708.t1 MTR_4g121560 40.057 352 181 9 6 331 108 455 2.19e-86 268
MS.gene008708.t1 MTR_4g126950 37.830 341 190 5 12 331 109 448 1.34e-84 263
MS.gene008708.t1 MTR_1g112210 36.826 334 185 7 12 329 59 382 2.26e-81 252
MS.gene008708.t1 MTR_5g059400 37.101 345 192 6 10 330 173 516 2.50e-81 256
MS.gene008708.t1 MTR_1g116130 37.101 345 192 6 10 330 173 516 2.50e-81 256
MS.gene008708.t1 MTR_5g033800 36.919 344 188 6 12 330 145 484 4.45e-81 254
MS.gene008708.t1 MTR_1g112210 36.826 334 172 8 12 329 59 369 6.48e-79 245
MS.gene008708.t1 MTR_8g069790 37.243 341 191 7 12 330 178 517 7.70e-79 250
MS.gene008708.t1 MTR_3g070900 37.356 348 188 8 11 330 105 450 1.95e-77 244
MS.gene008708.t1 MTR_4g016940 36.550 342 193 7 12 329 86 427 2.36e-77 243
MS.gene008708.t1 MTR_5g082880 37.677 353 183 11 11 330 164 512 2.92e-74 238
MS.gene008708.t1 MTR_7g109550 38.438 333 188 8 12 329 137 467 5.95e-74 236
MS.gene008708.t1 MTR_8g463790 36.152 343 192 8 12 330 71 410 2.25e-71 228
MS.gene008708.t1 MTR_4g014440 34.844 353 202 8 6 330 56 408 3.12e-71 227
MS.gene008708.t1 MTR_3g071650 34.402 343 201 8 12 330 89 431 7.19e-70 224
MS.gene008708.t1 MTR_7g070060 35.817 349 196 9 9 329 61 409 9.81e-69 220
MS.gene008708.t1 MTR_7g094750 35.411 353 196 9 6 330 85 433 7.45e-68 219
MS.gene008708.t1 MTR_3g090120 37.714 350 189 9 4 330 117 460 8.70e-68 219
MS.gene008708.t1 MTR_7g068920 35.362 345 199 7 9 329 77 421 2.83e-67 217
MS.gene008708.t1 MTR_1g058380 34.694 343 198 8 12 330 55 395 3.23e-67 216
MS.gene008708.t1 MTR_2g022560 34.770 348 202 8 7 330 92 438 4.66e-67 217
MS.gene008708.t1 MTR_5g083260 33.048 351 211 8 4 330 75 425 1.12e-65 213
MS.gene008708.t1 MTR_4g014430 34.097 349 203 9 5 329 101 446 1.74e-64 210
MS.gene008708.t1 MTR_8g093530 34.030 335 198 7 12 330 90 417 4.61e-63 206
MS.gene008708.t1 MTR_5g033800 35.865 237 134 4 12 233 145 378 1.78e-53 180
MS.gene008708.t1 MTR_7g070060 32.680 306 178 9 52 329 2 307 2.31e-47 162
MS.gene008708.t1 MTR_5g069320 33.981 309 135 8 39 322 122 386 3.04e-45 159
MS.gene008708.t1 MTR_7g094750 33.197 244 145 4 6 233 85 326 4.83e-45 156
MS.gene008708.t1 MTR_7g070060 33.755 237 147 4 9 235 61 297 2.22e-43 152
MS.gene008708.t1 MTR_5g035090 30.435 345 205 11 9 322 80 420 2.21e-39 144
MS.gene008708.t1 MTR_5g035150 29.714 350 213 10 11 329 84 431 7.24e-36 134
MS.gene008708.t1 MTR_6g016875 30.411 365 207 12 2 329 54 408 5.86e-35 132
MS.gene008708.t1 MTR_6g061710 28.375 363 200 12 12 329 167 514 6.61e-35 133
MS.gene008708.t1 MTR_8g093870 29.799 349 213 11 8 329 129 472 2.01e-34 131
MS.gene008708.t1 MTR_4g092830 28.775 351 213 13 12 330 48 393 2.32e-34 130
MS.gene008708.t1 MTR_6g016880 28.775 351 214 8 11 331 77 421 2.67e-34 130
MS.gene008708.t1 MTR_3g021150 55.660 106 46 1 196 300 1 106 6.47e-34 121
MS.gene008708.t1 MTR_4g125060 30.748 361 210 16 3 329 117 471 1.74e-33 129
MS.gene008708.t1 MTR_4g125060 30.748 361 210 16 3 329 156 510 3.00e-33 129
MS.gene008708.t1 MTR_4g125060 30.748 361 210 16 3 329 185 539 3.54e-33 129
MS.gene008708.t1 MTR_1g076690 27.809 356 209 12 9 325 220 566 4.02e-32 126
MS.gene008708.t1 MTR_7g019120 27.119 354 226 9 6 331 73 422 4.30e-32 124
MS.gene008708.t1 MTR_5g035080 28.255 361 201 16 12 329 75 420 1.21e-31 123
MS.gene008708.t1 MTR_8g027900 28.610 367 205 15 6 330 205 556 1.62e-31 124
MS.gene008708.t1 MTR_5g035070 26.724 348 224 9 12 329 87 433 7.16e-31 121
MS.gene008708.t1 MTR_3g109840 27.762 353 216 13 3 322 62 408 2.66e-30 119
MS.gene008708.t1 MTR_2g016060 28.032 371 217 15 1 331 43 403 5.33e-30 118
MS.gene008708.t1 MTR_6g007777 28.333 360 214 14 3 329 109 457 2.87e-29 117
MS.gene008708.t1 MTR_8g099300 26.629 353 225 9 9 330 71 420 6.62e-29 115
MS.gene008708.t1 MTR_7g033230 27.066 351 218 11 12 329 264 609 2.12e-28 115
MS.gene008708.t1 MTR_7g033230 27.066 351 218 11 12 329 334 679 4.80e-28 115
MS.gene008708.t1 MTR_8g104970 27.171 357 218 12 8 329 116 465 5.63e-28 113
MS.gene008708.t1 MTR_6g016240 27.247 356 219 12 8 329 67 416 4.08e-25 105
MS.gene008708.t1 MTR_7g019140 39.130 92 56 0 9 100 76 167 1.50e-18 82.0
MS.gene008708.t1 MTR_5g035120 40.659 91 54 0 12 102 75 165 1.07e-17 79.7
MS.gene008708.t1 MTR_3g021080 36.275 102 44 2 230 330 99 180 5.53e-16 75.1
MS.gene008708.t1 MTR_2g022580 29.600 125 74 3 72 182 2 126 4.34e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008708.t1 AT1G29050 65.361 332 113 2 1 331 50 380 1.55e-172 484
MS.gene008708.t1 AT2G34070 65.465 333 111 4 1 331 55 385 4.89e-167 470
MS.gene008708.t1 AT2G31110 58.491 318 132 0 12 329 45 362 8.60e-149 423
MS.gene008708.t1 AT2G42570 58.589 326 135 0 5 330 40 365 3.33e-147 419
MS.gene008708.t1 AT3G14850 52.744 328 150 3 7 330 29 355 2.73e-127 368
MS.gene008708.t1 AT2G30900 52.469 324 149 4 12 330 43 366 9.34e-124 360
MS.gene008708.t1 AT2G34070 67.063 252 80 3 82 331 1 251 1.08e-123 355
MS.gene008708.t1 AT1G78710 48.318 327 164 4 7 330 33 357 1.02e-120 352
MS.gene008708.t1 AT5G58600 50.000 338 152 5 10 330 64 401 2.48e-115 340
MS.gene008708.t1 AT2G30010 46.499 357 163 6 2 330 41 397 4.73e-108 321
MS.gene008708.t1 AT2G30010 46.499 357 163 6 2 330 84 440 7.93e-108 322
MS.gene008708.t1 AT2G31110 58.525 217 90 0 12 228 45 261 7.96e-100 295
MS.gene008708.t1 AT3G14850 53.755 253 112 3 82 330 1 252 1.48e-95 284
MS.gene008708.t1 AT2G31110 59.420 207 84 0 123 329 8 214 1.24e-93 278
MS.gene008708.t1 AT5G06700 40.698 344 180 6 11 331 254 596 8.57e-91 283
MS.gene008708.t1 AT3G12060 40.407 344 181 6 11 331 195 537 1.77e-87 273
MS.gene008708.t1 AT1G78710 40.673 327 136 5 7 330 26 297 3.72e-87 264
MS.gene008708.t1 AT2G30900 53.097 226 104 2 12 235 43 268 1.56e-85 259
MS.gene008708.t1 AT3G54260 38.806 335 179 7 12 330 51 375 2.83e-84 259
MS.gene008708.t1 AT5G20590 37.429 350 196 6 4 331 133 481 1.81e-83 261
MS.gene008708.t1 AT5G58600 51.316 228 109 2 10 235 64 291 1.55e-81 249
MS.gene008708.t1 AT5G01360 37.391 345 191 7 10 329 34 378 8.03e-81 251
MS.gene008708.t1 AT5G49340 37.283 346 191 8 11 331 99 443 1.44e-80 252
MS.gene008708.t1 AT5G01360 37.391 345 191 7 10 329 90 434 4.04e-80 251
MS.gene008708.t1 AT3G62390 38.012 342 192 8 7 330 133 472 9.66e-77 243
MS.gene008708.t1 AT1G60790 36.416 346 193 7 11 330 189 533 2.19e-76 244
MS.gene008708.t1 AT2G40150 35.493 355 193 9 10 330 69 421 4.60e-73 232
MS.gene008708.t1 AT3G55990 36.599 347 190 9 12 330 140 484 1.18e-72 233
MS.gene008708.t1 AT1G73140 35.920 348 196 8 7 330 62 406 1.71e-72 230
MS.gene008708.t1 AT5G19160 36.415 357 189 11 7 330 94 445 2.62e-72 231
MS.gene008708.t1 AT3G11030 35.127 353 202 9 4 330 98 449 6.49e-70 224
MS.gene008708.t1 AT3G06080 34.571 350 202 7 7 330 98 446 1.79e-69 224
MS.gene008708.t1 AT5G06230 33.811 349 203 7 9 330 18 365 2.06e-69 221
MS.gene008708.t1 AT1G48880 36.012 336 181 8 12 326 115 437 3.74e-69 223
MS.gene008708.t1 AT5G06230 33.811 349 203 7 9 330 59 406 5.85e-69 221
MS.gene008708.t1 AT2G40160 34.670 349 197 9 12 330 77 424 2.41e-67 217
MS.gene008708.t1 AT2G40160 34.384 349 198 9 12 330 77 424 3.25e-67 218
MS.gene008708.t1 AT5G01620 33.428 353 208 9 2 329 99 449 5.77e-67 217
MS.gene008708.t1 AT5G01620 33.428 353 208 9 2 329 99 449 5.77e-67 217
MS.gene008708.t1 AT5G01620 33.428 353 208 9 2 329 107 457 1.08e-66 216
MS.gene008708.t1 AT2G38320 33.718 347 202 8 12 330 59 405 1.49e-66 215
MS.gene008708.t1 AT2G40320 35.127 353 201 10 4 330 73 423 4.33e-66 214
MS.gene008708.t1 AT3G11570 34.259 324 191 6 28 330 98 420 2.84e-65 212
MS.gene008708.t1 AT2G38320 31.975 319 190 7 39 330 4 322 4.08e-56 185
MS.gene008708.t1 AT5G20590 35.656 244 143 4 4 233 133 376 3.03e-52 177
MS.gene008708.t1 AT5G19160 35.685 241 141 5 7 233 94 334 1.87e-51 174
MS.gene008708.t1 AT3G62390 35.565 239 142 5 7 233 133 371 3.31e-49 170
MS.gene008708.t1 AT2G40160 33.761 234 143 4 12 233 77 310 1.59e-46 160
MS.gene008708.t1 AT3G06080 33.610 241 146 4 7 233 98 338 4.55e-45 157
MS.gene008708.t1 AT5G06230 31.174 247 150 4 9 235 59 305 1.27e-41 147
MS.gene008708.t1 AT5G64470 31.111 360 195 16 12 331 55 401 3.93e-38 140
MS.gene008708.t1 AT5G64470 30.028 353 202 14 12 331 55 395 1.27e-37 139
MS.gene008708.t1 AT4G25360 29.888 358 207 12 6 325 169 520 9.62e-37 139
MS.gene008708.t1 AT4G25360 29.888 358 207 12 6 325 169 520 9.62e-37 139
MS.gene008708.t1 AT1G70230 28.739 341 212 12 12 329 79 411 1.05e-36 136
MS.gene008708.t1 AT5G64020 29.330 358 207 12 12 329 57 408 4.40e-36 135
MS.gene008708.t1 AT1G01430 27.348 362 224 10 4 330 91 448 4.40e-35 133
MS.gene008708.t1 AT5G51640 29.609 358 205 12 12 329 138 488 2.19e-34 132
MS.gene008708.t1 AT2G37720 29.096 354 201 13 12 329 143 482 2.38e-33 129
MS.gene008708.t1 AT5G20680 28.116 345 216 14 12 329 118 457 6.77e-32 124
MS.gene008708.t1 AT5G20680 28.116 345 216 14 12 329 194 533 1.60e-31 124
MS.gene008708.t1 AT5G20680 28.116 345 216 14 12 329 212 551 1.74e-31 124
MS.gene008708.t1 AT5G20680 28.116 345 216 14 12 329 212 551 1.74e-31 124
MS.gene008708.t1 AT5G20680 28.116 345 216 14 12 329 212 551 1.74e-31 124
MS.gene008708.t1 AT5G20680 28.116 345 216 14 12 329 212 551 1.74e-31 124
MS.gene008708.t1 AT5G15900 27.714 350 218 9 10 329 65 409 1.72e-30 120
MS.gene008708.t1 AT5G15900 27.714 350 218 9 10 329 84 428 2.05e-30 120
MS.gene008708.t1 AT3G02440 27.011 348 218 10 12 329 127 468 1.12e-29 118
MS.gene008708.t1 AT3G02440 27.011 348 218 10 12 329 135 476 1.14e-29 118
MS.gene008708.t1 AT5G15890 28.363 342 215 10 12 329 187 522 1.74e-29 118
MS.gene008708.t1 AT3G28150 28.333 360 197 12 12 329 70 410 5.52e-29 115
MS.gene008708.t1 AT4G01080 28.324 346 212 12 9 325 88 426 7.89e-29 115
MS.gene008708.t1 AT2G14530 27.528 356 215 12 9 329 59 406 2.35e-28 114
MS.gene008708.t1 AT4G11090 27.577 359 209 13 12 330 79 426 1.31e-27 112
MS.gene008708.t1 AT4G23790 26.420 352 224 10 12 330 79 428 5.39e-25 104
MS.gene008708.t1 AT5G64470 28.516 256 147 9 12 241 55 300 1.94e-21 93.2
MS.gene008708.t1 AT5G64470 28.516 256 147 9 12 241 55 300 2.45e-21 93.2

Find 84 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAATTGTAATTTGTTCTT+TGG 0.220399 4.4:-25103102 MS.gene008708:CDS
ACTATACCGTACACCATATT+TGG 0.252473 4.4:-25100957 MS.gene008708:CDS
TGAATTATATCCACCAAATA+TGG 0.255317 4.4:+25100944 None:intergenic
TATTTAAGATACTGATTATC+AGG 0.276183 4.4:+25102988 None:intergenic
ATTGACCACTTGGGCTGGTT+GGG 0.323556 4.4:-25100491 MS.gene008708:CDS
TTGTTCACTACATCATTTGC+AGG 0.336870 4.4:+25100224 None:intergenic
AAAACTAAACTCTTCTTTCA+AGG 0.357639 4.4:-25100445 MS.gene008708:CDS
CGGGGAAGTTGAACCATTAC+CGG 0.374830 4.4:-25100273 MS.gene008708:CDS
AGATTGCATGCACAGGACTA+TGG 0.376697 4.4:-25100992 MS.gene008708:intron
GAAAAGCTTGTAGTGACATT+AGG 0.381141 4.4:+25102565 None:intergenic
AGGAGTATGTTCCAAGTATC+AGG 0.381319 4.4:+25100062 None:intergenic
ATCAGTATCTTAAATACTCT+TGG 0.387106 4.4:-25102981 MS.gene008708:CDS
AATGTCTACTGTCTTCTTCC+AGG 0.394880 4.4:-25102530 MS.gene008708:intron
CTGGGTAAAGCACATGAATC+AGG 0.414998 4.4:+25102955 None:intergenic
AGTGAGGGACATGGACCGTT+TGG 0.425551 4.4:-25100530 MS.gene008708:CDS
TCCCCGCTACATGTCTTCTT+CGG 0.430209 4.4:+25100290 None:intergenic
GATTGACCACTTGGGCTGGT+TGG 0.439990 4.4:-25100492 MS.gene008708:CDS
GATAAGAAGATAATGTTTGT+AGG 0.445963 4.4:-25102646 MS.gene008708:CDS
GGGGCAGGATATGTGGTTCC+CGG 0.448211 4.4:+25100254 None:intergenic
GGATATGTGGTTCCCGGTAA+TGG 0.448546 4.4:+25100260 None:intergenic
TTGAATCCAGAGGGCAGGAT+TGG 0.450495 4.4:-25100319 MS.gene008708:intron
TTACTTACTTATTATGAGTA+GGG 0.455719 4.4:+25100416 None:intergenic
GTAATTTGTTCTTTGGAAGC+TGG 0.456024 4.4:-25103095 MS.gene008708:CDS
TTTATAAGGGATTGACCACT+TGG 0.465985 4.4:-25100501 MS.gene008708:CDS
ACATGATAAGAGCTGCATAT+AGG 0.466871 4.4:+25100042 None:intergenic
TCTTCAACTCCTGGCATTGG+TGG 0.467165 4.4:-25100847 MS.gene008708:CDS
ACATCATTTGCAGGAGGTAG+AGG 0.472593 4.4:+25100233 None:intergenic
TGTAGTCATTGGTGCCTACC+AGG 0.474740 4.4:-25100089 MS.gene008708:CDS
TTGTGCCCTTGCTGGGTGCA+TGG 0.478358 4.4:+25103131 None:intergenic
TACCAGGACTACCTGATACT+TGG 0.478567 4.4:-25100073 MS.gene008708:CDS
TAATTTGTTCTTTGGAAGCT+GGG 0.484946 4.4:-25103094 MS.gene008708:CDS
TGGTGTACGGTATAGTTGTA+TGG 0.491607 4.4:+25100964 None:intergenic
GGATCCTTAGTATAGATAGA+AGG 0.493596 4.4:+25100125 None:intergenic
AAGGGATTGACCACTTGGGC+TGG 0.495484 4.4:-25100496 MS.gene008708:CDS
TGATCTTCAACTCCTGGCAT+TGG 0.498503 4.4:-25100850 MS.gene008708:CDS
GTGGTGTAGGTTCAGTGGAG+AGG 0.499875 4.4:-25102689 MS.gene008708:intron
GATATAATTCAAGAAGATGT+TGG 0.503344 4.4:-25100932 MS.gene008708:CDS
GTTAAAGAACATGAAGAAGC+AGG 0.504244 4.4:-25100198 MS.gene008708:CDS
ATCTATACTAAGGATCCAAC+TGG 0.504484 4.4:-25100119 MS.gene008708:CDS
CTGGCATTGATTGTAGTCAT+TGG 0.505796 4.4:-25100100 MS.gene008708:CDS
GTCTTGTATGATTCATGCGT+CGG 0.512224 4.4:-25102590 MS.gene008708:CDS
TCTCCTTGAATCCAGAGGGC+AGG 0.517922 4.4:-25100324 MS.gene008708:intron
TTTGCAGGAGGTAGAGGGGC+AGG 0.519222 4.4:+25100239 None:intergenic
ACATCCTTCTATCTATACTA+AGG 0.519367 4.4:-25100129 MS.gene008708:CDS
AGGTAGAGGGGCAGGATATG+TGG 0.520452 4.4:+25100247 None:intergenic
GTTACTTACTTATTATGAGT+AGG 0.522689 4.4:+25100415 None:intergenic
TTCCAAGTATCAGGTAGTCC+TGG 0.535659 4.4:+25100071 None:intergenic
CTATAAAAGCTGGTAAAACA+TGG 0.540164 4.4:-25100889 MS.gene008708:CDS
CATCATTTGCAGGAGGTAGA+GGG 0.540823 4.4:+25100234 None:intergenic
AACCGAAGAAGACATGTAGC+GGG 0.541404 4.4:-25100292 MS.gene008708:CDS
ACATGTTGATCTTCAACTCC+TGG 0.546418 4.4:-25100856 MS.gene008708:CDS
AGTGTGGATCCACCAATGCC+AGG 0.547954 4.4:+25100838 None:intergenic
ATCAAACTGAGCTTCAACGA+AGG 0.553255 4.4:+25103029 None:intergenic
TGACTACAATCAATGCCAGT+TGG 0.558982 4.4:+25100104 None:intergenic
ATACCGTACACCATATTTGG+TGG 0.560969 4.4:-25100954 MS.gene008708:CDS
TACTTACTTATTATGAGTAG+GGG 0.562023 4.4:+25100417 None:intergenic
CCTTATAAAATGCATCCAAA+CGG 0.562648 4.4:+25100515 None:intergenic
CTGATTCATGTGCTTTACCC+AGG 0.569924 4.4:-25102954 MS.gene008708:intron
TGGCATTGGTGGATCCACAC+TGG 0.570664 4.4:-25100836 MS.gene008708:CDS
ACGCTGGACTCTATAAAAGC+TGG 0.571735 4.4:-25100899 MS.gene008708:CDS
ATTCATTGAGTCTAAACATG+TGG 0.578501 4.4:-25102621 MS.gene008708:CDS
TATCCACCAAATATGGTGTA+CGG 0.590876 4.4:+25100951 None:intergenic
GGGGAAGTTGAACCATTACC+GGG 0.597940 4.4:-25100272 MS.gene008708:CDS
TTATATCCATGCACCCAGCA+AGG 0.602227 4.4:-25103137 None:intergenic
TATATCCATGCACCCAGCAA+GGG 0.606783 4.4:-25103136 None:intergenic
TTCATTGAGTCTAAACATGT+GGG 0.609808 4.4:-25102620 MS.gene008708:CDS
TCAAACTGAGCTTCAACGAA+GGG 0.618613 4.4:+25103030 None:intergenic
ATCATTTGCAGGAGGTAGAG+GGG 0.621851 4.4:+25100235 None:intergenic
ATCAACCCAACCAGCCCAAG+TGG 0.622283 4.4:+25100486 None:intergenic
GAGATGGTTCTAATCTAGTG+AGG 0.627215 4.4:-25100546 MS.gene008708:CDS
AAGTATCAGGTAGTCCTGGT+AGG 0.628734 4.4:+25100075 None:intergenic
AGATGGTTCTAATCTAGTGA+GGG 0.631565 4.4:-25100545 MS.gene008708:CDS
TTCACTACATCATTTGCAGG+AGG 0.631894 4.4:+25100227 None:intergenic
TGTTGGTAGAGTGCTTACGC+TGG 0.633724 4.4:-25100915 MS.gene008708:CDS
TTATAAGGGATTGACCACTT+GGG 0.640369 4.4:-25100500 MS.gene008708:CDS
GAACCGAAGAAGACATGTAG+CGG 0.645973 4.4:-25100293 MS.gene008708:CDS
CAGCTTGAAGAATCATAGAG+AGG 0.652645 4.4:+25103054 None:intergenic
TTCTAATCTAGTGAGGGACA+TGG 0.659068 4.4:-25100539 MS.gene008708:CDS
TGGAAGAAGACAGTAGACAT+TGG 0.660692 4.4:+25102532 None:intergenic
TGGTAAAACATGGTTAGACA+TGG 0.686448 4.4:-25100879 MS.gene008708:CDS
ATCCACACTGGAGATTTACG+AGG 0.690791 4.4:-25100824 MS.gene008708:CDS
ACCGAAGAAGACATGTAGCG+GGG 0.713042 4.4:-25100291 MS.gene008708:CDS
TCCACACTGGAGATTTACGA+GGG 0.713392 4.4:-25100823 MS.gene008708:intron
ACCCTCGTAAATCTCCAGTG+TGG 0.727033 4.4:+25100822 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATATTTTAATTATATAT+AGG + chr4.4:25101136-25101155 None:intergenic 0.0%
!! TAAAAAAAAATCAAATATGA+AGG + chr4.4:25101907-25101926 None:intergenic 10.0%
!! TATATAATAATATTAAAGTG+AGG - chr4.4:25101160-25101179 MS.gene008708:intron 10.0%
!!! TATTTTAATTATATATAGGT+CGG + chr4.4:25101132-25101151 None:intergenic 10.0%
!! AAAATTTGTATTGTCATTAT+AGG - chr4.4:25101710-25101729 MS.gene008708:intron 15.0%
!! AAATTTGTATTGTCATTATA+GGG - chr4.4:25101711-25101730 MS.gene008708:intron 15.0%
!! ATCATTGATATTATCATATT+GGG + chr4.4:25102050-25102069 None:intergenic 15.0%
!! CATTAATTCATTATAATAGA+TGG - chr4.4:25101845-25101864 MS.gene008708:intron 15.0%
!! TATATGTGACAATTAATATT+TGG - chr4.4:25100389-25100408 MS.gene008708:intron 15.0%
!! TATCATTGATATTATCATAT+TGG + chr4.4:25102051-25102070 None:intergenic 15.0%
!!! AATAAAGTAAAGCTTTAATT+TGG + chr4.4:25101968-25101987 None:intergenic 15.0%
!!! AATAATTGTAATTTGTTCTT+TGG - chr4.4:25100067-25100086 MS.gene008708:CDS 15.0%
!!! AGACTTATTTTTTTTAAGTT+TGG + chr4.4:25101367-25101386 None:intergenic 15.0%
!!! ATAAAGTAAAGCTTTAATTT+GGG + chr4.4:25101967-25101986 None:intergenic 15.0%
!!! TCATTGATTTTTTTTTTTGA+AGG + chr4.4:25102082-25102101 None:intergenic 15.0%
!! AATATCTAGATTTAGTGATA+AGG + chr4.4:25101253-25101272 None:intergenic 20.0%
!! AATTAACTCGTTGAATAAAA+TGG - chr4.4:25101024-25101043 MS.gene008708:intron 20.0%
!! AATTTGTATTGTCATTATAG+GGG - chr4.4:25101712-25101731 MS.gene008708:intron 20.0%
!! AGATAAAAAAATATGTTGCA+AGG + chr4.4:25101791-25101810 None:intergenic 20.0%
!! GATAAAAAAATATGTTGCAA+GGG + chr4.4:25101790-25101809 None:intergenic 20.0%
!! TCATTGATATTATCATATTG+GGG + chr4.4:25102049-25102068 None:intergenic 20.0%
!! TTACTTACTTATTATGAGTA+GGG + chr4.4:25102756-25102775 None:intergenic 20.0%
!! TTCAAATATACATTCAATTG+TGG + chr4.4:25102411-25102430 None:intergenic 20.0%
!! TTGAATGTATATTTGAATTG+AGG - chr4.4:25102414-25102433 MS.gene008708:intron 20.0%
!!! AAATTTGTTTCTTACTATAG+TGG - chr4.4:25100304-25100323 MS.gene008708:CDS 20.0%
!!! AATATTTCTATGGAAAATGA+TGG + chr4.4:25100941-25100960 None:intergenic 20.0%
!!! ACTTTATTTCTATCCATATA+GGG - chr4.4:25101980-25101999 MS.gene008708:intron 20.0%
!!! ATTGGACCATAATTTTAAAA+AGG - chr4.4:25101336-25101355 MS.gene008708:intron 20.0%
!!! GTAATGAGTAATTATTTTTG+TGG - chr4.4:25100461-25100480 MS.gene008708:CDS 20.0%
!!! TACTTTATTTCTATCCATAT+AGG - chr4.4:25101979-25101998 MS.gene008708:intron 20.0%
!!! TATTTAAGATACTGATTATC+AGG + chr4.4:25100184-25100203 None:intergenic 20.0%
!!! TTTTAAAGAAGCTAATAGAT+TGG - chr4.4:25101318-25101337 MS.gene008708:intron 20.0%
! AAAACTAAACTCTTCTTTCA+AGG - chr4.4:25102724-25102743 MS.gene008708:intron 25.0%
! AATTTCGAGCTTAATATCTT+TGG + chr4.4:25100895-25100914 None:intergenic 25.0%
! AGAAATATTTGAATGAGACT+AGG - chr4.4:25100952-25100971 MS.gene008708:CDS 25.0%
! ATCAGTATCTTAAATACTCT+TGG - chr4.4:25100188-25100207 MS.gene008708:CDS 25.0%
! CACTAACACAGTTTAATTAT+GGG + chr4.4:25102147-25102166 None:intergenic 25.0%
! CAGTTTGATTGCAAAAAATA+TGG - chr4.4:25100154-25100173 MS.gene008708:CDS 25.0%
! GATAAAAATATCCATATTCG+TGG - chr4.4:25102005-25102024 MS.gene008708:intron 25.0%
! GATAAGAAGATAATGTTTGT+AGG - chr4.4:25100523-25100542 MS.gene008708:CDS 25.0%
! GATATAATTCAAGAAGATGT+TGG - chr4.4:25102237-25102256 MS.gene008708:intron 25.0%
! GTTACTTACTTATTATGAGT+AGG + chr4.4:25102757-25102776 None:intergenic 25.0%
! TAACCTATTTAAACAAAGTC+TGG - chr4.4:25101441-25101460 MS.gene008708:intron 25.0%
! TACTTACTTATTATGAGTAG+GGG + chr4.4:25102755-25102774 None:intergenic 25.0%
! TATATGTGACATATCACAAA+GGG + chr4.4:25100279-25100298 None:intergenic 25.0%
! TCACTAACACAGTTTAATTA+TGG + chr4.4:25102148-25102167 None:intergenic 25.0%
! TGAATTATATCCACCAAATA+TGG + chr4.4:25102228-25102247 None:intergenic 25.0%
! TTACATTCGTACTATACATT+AGG + chr4.4:25100445-25100464 None:intergenic 25.0%
! TTATATGTGACATATCACAA+AGG + chr4.4:25100280-25100299 None:intergenic 25.0%
!! AAAGTGATGAATGTGTTATT+TGG - chr4.4:25102572-25102591 MS.gene008708:CDS 25.0%
!! CTCTAATTAAAAGTACGTAT+TGG + chr4.4:25102803-25102822 None:intergenic 25.0%
!! GTCTCATTCAAATATTTCTA+TGG + chr4.4:25100951-25100970 None:intergenic 25.0%
!! TTTAGAGTTGTCTTTACATA+AGG + chr4.4:25101613-25101632 None:intergenic 25.0%
!!! AAGAAGAGTTTAGTTTTAGT+AGG + chr4.4:25102721-25102740 None:intergenic 25.0%
!!! AGAAGAGTTTAGTTTTAGTA+GGG + chr4.4:25102720-25102739 None:intergenic 25.0%
!!! AGTAATTATTTTTGTGGTGT+AGG - chr4.4:25100467-25100486 MS.gene008708:CDS 25.0%
!!! ATAAACGTGTTTTGTTTCTA+TGG + chr4.4:25100360-25100379 None:intergenic 25.0%
!!! ATATTTTTATCCACCCTATA+TGG + chr4.4:25101996-25102015 None:intergenic 25.0%
!!! GCTTGACCTTTTTAAAATTA+TGG + chr4.4:25101345-25101364 None:intergenic 25.0%
!!! TCATTTTGTTTGTTTCTTTG+CGG - chr4.4:25100735-25100754 MS.gene008708:intron 25.0%
!!! TGTGTAAAGACTTTTGAAAA+AGG + chr4.4:25101290-25101309 None:intergenic 25.0%
AAACAAGAACAACTATTACC+TGG + chr4.4:25100660-25100679 None:intergenic 30.0%
ACATCCTTCTATCTATACTA+AGG - chr4.4:25103040-25103059 MS.gene008708:CDS 30.0%
ATACAAACTAACAGTATACC+TGG + chr4.4:25100236-25100255 None:intergenic 30.0%
ATTCATTGAGTCTAAACATG+TGG - chr4.4:25100548-25100567 MS.gene008708:CDS 30.0%
ATTTGAATTGAGGTGATTTG+AGG - chr4.4:25102424-25102443 MS.gene008708:intron 30.0%
CCTGTTACATGTAAAATGAT+AGG - chr4.4:25100840-25100859 MS.gene008708:CDS 30.0%
CCTTATAAAATGCATCCAAA+CGG + chr4.4:25102657-25102676 None:intergenic 30.0%
CTATAAAAGCTGGTAAAACA+TGG - chr4.4:25102280-25102299 MS.gene008708:intron 30.0%
GATATTAAGCTCGAAATTGA+AGG - chr4.4:25100897-25100916 MS.gene008708:CDS 30.0%
TAAACTGTGTTAGTGATACT+TGG - chr4.4:25102152-25102171 MS.gene008708:intron 30.0%
TAACACAGTTTAATTATGGG+TGG + chr4.4:25102144-25102163 None:intergenic 30.0%
TACAAACTAACAGTATACCT+GGG + chr4.4:25100235-25100254 None:intergenic 30.0%
TATTTGAATGAGACTAGGTT+AGG - chr4.4:25100957-25100976 MS.gene008708:CDS 30.0%
TTCATTGAGTCTAAACATGT+GGG - chr4.4:25100549-25100568 MS.gene008708:CDS 30.0%
TTGTAAACTGTTGCAATCAT+AGG + chr4.4:25101505-25101524 None:intergenic 30.0%
! AGTCCAGACTTTGTTTAAAT+AGG + chr4.4:25101447-25101466 None:intergenic 30.0%
! ATCAGCTATAAACTTTTGTC+GGG + chr4.4:25100986-25101005 None:intergenic 30.0%
! CCTATCATTTTACATGTAAC+AGG + chr4.4:25100843-25100862 None:intergenic 30.0%
! CGTTTGGATGCATTTTATAA+GGG - chr4.4:25102655-25102674 MS.gene008708:CDS 30.0%
! GATCAGCTATAAACTTTTGT+CGG + chr4.4:25100987-25101006 None:intergenic 30.0%
!! CATTTTGTTTGTTTCTTTGC+GGG - chr4.4:25100736-25100755 MS.gene008708:intron 30.0%
!! TAATTTGTTCTTTGGAAGCT+GGG - chr4.4:25100075-25100094 MS.gene008708:CDS 30.0%
!! TTATTTCTATCCATATAGGG+TGG - chr4.4:25101983-25102002 MS.gene008708:intron 30.0%
ACATGATAAGAGCTGCATAT+AGG + chr4.4:25103130-25103149 None:intergenic 35.0%
ACTATACCGTACACCATATT+TGG - chr4.4:25102212-25102231 MS.gene008708:intron 35.0%
ATCTATACTAAGGATCCAAC+TGG - chr4.4:25103050-25103069 MS.gene008708:CDS 35.0%
GAAAAGCTTGTAGTGACATT+AGG + chr4.4:25100607-25100626 None:intergenic 35.0%
GATTGCAACAGTTTACAAGT+TGG - chr4.4:25101507-25101526 MS.gene008708:intron 35.0%
GGATCCTTAGTATAGATAGA+AGG + chr4.4:25103047-25103066 None:intergenic 35.0%
GTGTCATCAACATCAATCAT+GGG - chr4.4:25101564-25101583 MS.gene008708:intron 35.0%
GTTAAAGAACATGAAGAAGC+AGG - chr4.4:25102971-25102990 MS.gene008708:CDS 35.0%
TATCATATTGGGGATAGCAA+TGG + chr4.4:25102039-25102058 None:intergenic 35.0%
TATCCACCAAATATGGTGTA+CGG + chr4.4:25102221-25102240 None:intergenic 35.0%
TATCCATATTCGTGGATATC+CGG - chr4.4:25102013-25102032 MS.gene008708:intron 35.0%
TGGTAAAACATGGTTAGACA+TGG - chr4.4:25102290-25102309 MS.gene008708:intron 35.0%
TGTGTCATCAACATCAATCA+TGG - chr4.4:25101563-25101582 MS.gene008708:intron 35.0%
TTATAAGGGATTGACCACTT+GGG - chr4.4:25102669-25102688 MS.gene008708:CDS 35.0%
TTGTTCACTACATCATTTGC+AGG + chr4.4:25102948-25102967 None:intergenic 35.0%
TTTATAAGGGATTGACCACT+TGG - chr4.4:25102668-25102687 MS.gene008708:CDS 35.0%
! ATGTCACTACAAGCTTTTCT+AGG - chr4.4:25100608-25100627 MS.gene008708:intron 35.0%
! CCGTTTGGATGCATTTTATA+AGG - chr4.4:25102654-25102673 MS.gene008708:CDS 35.0%
! TCGGTTTGACATGCATAATA+TGG + chr4.4:25101113-25101132 None:intergenic 35.0%
!! AGATGGTTCTAATCTAGTGA+GGG - chr4.4:25102624-25102643 MS.gene008708:CDS 35.0%
!! GACTTTTGAAAAAGGCTAAC+AGG + chr4.4:25101282-25101301 None:intergenic 35.0%
!! GTAATTTGTTCTTTGGAAGC+TGG - chr4.4:25100074-25100093 MS.gene008708:CDS 35.0%
!! TTATTTGGCTTGCTTCATTC+AGG - chr4.4:25102587-25102606 MS.gene008708:CDS 35.0%
!!! GAGAGGATTTTTTGAATAGG+TGG - chr4.4:25100497-25100516 MS.gene008708:CDS 35.0%
!!! GGATTTTTTGAATAGGTGGA+AGG - chr4.4:25100501-25100520 MS.gene008708:CDS 35.0%
!!! GTGGAGAGGATTTTTTGAAT+AGG - chr4.4:25100494-25100513 MS.gene008708:CDS 35.0%
AATGTCTACTGTCTTCTTCC+AGG - chr4.4:25100639-25100658 MS.gene008708:intron 40.0%
ACATGTTGATCTTCAACTCC+TGG - chr4.4:25102313-25102332 MS.gene008708:intron 40.0%
ATACCGTACACCATATTTGG+TGG - chr4.4:25102215-25102234 MS.gene008708:intron 40.0%
ATCAAACTGAGCTTCAACGA+AGG + chr4.4:25100143-25100162 None:intergenic 40.0%
CAGCTTGAAGAATCATAGAG+AGG + chr4.4:25100118-25100137 None:intergenic 40.0%
CATCAATCATGGGTATGCTT+TGG - chr4.4:25101574-25101593 MS.gene008708:intron 40.0%
GAGGTGATTTGAGGAAACTA+CGG - chr4.4:25102433-25102452 MS.gene008708:intron 40.0%
GTCTTGTATGATTCATGCGT+CGG - chr4.4:25100579-25100598 MS.gene008708:intron 40.0%
TATTATCACCCTCAAACGAG+AGG - chr4.4:25101472-25101491 MS.gene008708:intron 40.0%
TCAAACTGAGCTTCAACGAA+GGG + chr4.4:25100142-25100161 None:intergenic 40.0%
TCTCGTTTGAGGGTGATAAT+AGG + chr4.4:25101473-25101492 None:intergenic 40.0%
TGACTACAATCAATGCCAGT+TGG + chr4.4:25103068-25103087 None:intergenic 40.0%
TGGAAGAAGACAGTAGACAT+TGG + chr4.4:25100640-25100659 None:intergenic 40.0%
TGGTGTACGGTATAGTTGTA+TGG + chr4.4:25102208-25102227 None:intergenic 40.0%
TTCACTACATCATTTGCAGG+AGG + chr4.4:25102945-25102964 None:intergenic 40.0%
TTCTAATCTAGTGAGGGACA+TGG - chr4.4:25102630-25102649 MS.gene008708:CDS 40.0%
! AGGAGTATGTTCCAAGTATC+AGG + chr4.4:25103110-25103129 None:intergenic 40.0%
! AGGTGGGATTTTATCAGAGA+TGG - chr4.4:25102607-25102626 MS.gene008708:CDS 40.0%
! TGCAACAGTTTACAAGTTGG+AGG - chr4.4:25101510-25101529 MS.gene008708:intron 40.0%
! TTTTTGTGGTGTAGGTTCAG+TGG - chr4.4:25100475-25100494 MS.gene008708:CDS 40.0%
!! CTGGCATTGATTGTAGTCAT+TGG - chr4.4:25103069-25103088 MS.gene008708:CDS 40.0%
!! GAGATGGTTCTAATCTAGTG+AGG - chr4.4:25102623-25102642 MS.gene008708:CDS 40.0%
AACCGAAGAAGACATGTAGC+GGG - chr4.4:25102877-25102896 MS.gene008708:intron 45.0%
AAGTATCAGGTAGTCCTGGT+AGG + chr4.4:25103097-25103116 None:intergenic 45.0%
ACATCATTTGCAGGAGGTAG+AGG + chr4.4:25102939-25102958 None:intergenic 45.0%
ACGCTGGACTCTATAAAAGC+TGG - chr4.4:25102270-25102289 MS.gene008708:intron 45.0%
AGATTGCATGCACAGGACTA+TGG - chr4.4:25102177-25102196 MS.gene008708:intron 45.0%
ATATTGGGGATAGCAATGGC+CGG + chr4.4:25102035-25102054 None:intergenic 45.0%
ATCATTTGCAGGAGGTAGAG+GGG + chr4.4:25102937-25102956 None:intergenic 45.0%
ATCCACACTGGAGATTTACG+AGG - chr4.4:25102345-25102364 MS.gene008708:intron 45.0%
CATCATTTGCAGGAGGTAGA+GGG + chr4.4:25102938-25102957 None:intergenic 45.0%
CCTTTCTCCTTGAATCCAGA+GGG - chr4.4:25102841-25102860 MS.gene008708:intron 45.0%
CTGATTCATGTGCTTTACCC+AGG - chr4.4:25100215-25100234 MS.gene008708:CDS 45.0%
CTGGGTAAAGCACATGAATC+AGG + chr4.4:25100217-25100236 None:intergenic 45.0%
GAACCGAAGAAGACATGTAG+CGG - chr4.4:25102876-25102895 MS.gene008708:intron 45.0%
GTTGACATCCTCTCGTTTGA+GGG + chr4.4:25101483-25101502 None:intergenic 45.0%
TACCAGGACTACCTGATACT+TGG - chr4.4:25103096-25103115 MS.gene008708:CDS 45.0%
TCCACACTGGAGATTTACGA+GGG - chr4.4:25102346-25102365 MS.gene008708:intron 45.0%
TCCTTTCTCCTTGAATCCAG+AGG - chr4.4:25102840-25102859 MS.gene008708:intron 45.0%
TGATCTTCAACTCCTGGCAT+TGG - chr4.4:25102319-25102338 MS.gene008708:intron 45.0%
TTCCAAGTATCAGGTAGTCC+TGG + chr4.4:25103101-25103120 None:intergenic 45.0%
! TTGACTTTTGTGCCCTTGCT+GGG + chr4.4:25100048-25100067 None:intergenic 45.0%
!! TTGGCTTGCTTCATTCAGGT+GGG - chr4.4:25102591-25102610 MS.gene008708:CDS 45.0%
!! TTTGACTTTTGTGCCCTTGC+TGG + chr4.4:25100049-25100068 None:intergenic 45.0%
!! TTTGGCTTGCTTCATTCAGG+TGG - chr4.4:25102590-25102609 MS.gene008708:CDS 45.0%
!!! ATGTTTTAAATTTTTAAATA+AGG + chr4.4:25101815-25101834 None:intergenic 5.0%
AATCCTGCCCTCTGGATTCA+AGG + chr4.4:25102851-25102870 None:intergenic 50.0%
ACCCTCGTAAATCTCCAGTG+TGG + chr4.4:25102350-25102369 None:intergenic 50.0%
ACCGAAGAAGACATGTAGCG+GGG - chr4.4:25102878-25102897 MS.gene008708:intron 50.0%
CCCTCTGGATTCAAGGAGAA+AGG + chr4.4:25102844-25102863 None:intergenic 50.0%
CGGGGAAGTTGAACCATTAC+CGG - chr4.4:25102896-25102915 MS.gene008708:intron 50.0%
CTTGGAGAGATTGCATGCAC+AGG - chr4.4:25102170-25102189 MS.gene008708:intron 50.0%
GGATATGTGGTTCCCGGTAA+TGG + chr4.4:25102912-25102931 None:intergenic 50.0%
GGGGAAGTTGAACCATTACC+GGG - chr4.4:25102897-25102916 MS.gene008708:intron 50.0%
GGTTGACATCCTCTCGTTTG+AGG + chr4.4:25101484-25101503 None:intergenic 50.0%
TCCCCGCTACATGTCTTCTT+CGG + chr4.4:25102882-25102901 None:intergenic 50.0%
TGGCCGGATATCCACGAATA+TGG + chr4.4:25102019-25102038 None:intergenic 50.0%
TTCATTCCAATCCTGCCCTC+TGG + chr4.4:25102859-25102878 None:intergenic 50.0%
TTGAATCCAGAGGGCAGGAT+TGG - chr4.4:25102850-25102869 MS.gene008708:intron 50.0%
! TCTTCAACTCCTGGCATTGG+TGG - chr4.4:25102322-25102341 MS.gene008708:intron 50.0%
! TGTAGTCATTGGTGCCTACC+AGG - chr4.4:25103080-25103099 MS.gene008708:CDS 50.0%
! TGTTGGTAGAGTGCTTACGC+TGG - chr4.4:25102254-25102273 MS.gene008708:intron 50.0%
!! ATTGACCACTTGGGCTGGTT+GGG - chr4.4:25102678-25102697 MS.gene008708:CDS 50.0%
AAGGGATTGACCACTTGGGC+TGG - chr4.4:25102673-25102692 MS.gene008708:CDS 55.0%
AGGTAGAGGGGCAGGATATG+TGG + chr4.4:25102925-25102944 None:intergenic 55.0%
AGTGAGGGACATGGACCGTT+TGG - chr4.4:25102639-25102658 MS.gene008708:CDS 55.0%
AGTGTGGATCCACCAATGCC+AGG + chr4.4:25102334-25102353 None:intergenic 55.0%
ATCAACCCAACCAGCCCAAG+TGG + chr4.4:25102686-25102705 None:intergenic 55.0%
GTGGTGTAGGTTCAGTGGAG+AGG - chr4.4:25100480-25100499 MS.gene008708:CDS 55.0%
TCTCCTTGAATCCAGAGGGC+AGG - chr4.4:25102845-25102864 MS.gene008708:intron 55.0%
!! GATTGACCACTTGGGCTGGT+TGG - chr4.4:25102677-25102696 MS.gene008708:CDS 55.0%
!! TGGCATTGGTGGATCCACAC+TGG - chr4.4:25102333-25102352 MS.gene008708:intron 55.0%
GGGGCAGGATATGTGGTTCC+CGG + chr4.4:25102918-25102937 None:intergenic 60.0%
TTTGCAGGAGGTAGAGGGGC+AGG + chr4.4:25102933-25102952 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 25100040 25103151 25100040 ID=MS.gene008708
chr4.4 mRNA 25100040 25103151 25100040 ID=MS.gene008708.t1;Parent=MS.gene008708
chr4.4 exon 25102955 25103151 25102955 ID=MS.gene008708.t1.exon1;Parent=MS.gene008708.t1
chr4.4 CDS 25102955 25103151 25102955 ID=cds.MS.gene008708.t1;Parent=MS.gene008708.t1
chr4.4 exon 25102531 25102702 25102531 ID=MS.gene008708.t1.exon2;Parent=MS.gene008708.t1
chr4.4 CDS 25102531 25102702 25102531 ID=cds.MS.gene008708.t1;Parent=MS.gene008708.t1
chr4.4 exon 25100824 25100999 25100824 ID=MS.gene008708.t1.exon3;Parent=MS.gene008708.t1
chr4.4 CDS 25100824 25100999 25100824 ID=cds.MS.gene008708.t1;Parent=MS.gene008708.t1
chr4.4 exon 25100424 25100582 25100424 ID=MS.gene008708.t1.exon4;Parent=MS.gene008708.t1
chr4.4 CDS 25100424 25100582 25100424 ID=cds.MS.gene008708.t1;Parent=MS.gene008708.t1
chr4.4 exon 25100040 25100331 25100040 ID=MS.gene008708.t1.exon5;Parent=MS.gene008708.t1
chr4.4 CDS 25100040 25100331 25100040 ID=cds.MS.gene008708.t1;Parent=MS.gene008708.t1
Gene Sequence

>MS.gene008708

ATGCACCCAGCAAGGGCACAAAAGTCAAATAATTGTAATTTGTTCTTTGGAAGCTGGGTGATTGACTCTTCATATCCTCTCTATGATTCTTCAAGCTGTCCCTTCGTTGAAGCTCAGTTTGATTGCAAAAAATATGGAAGACCTGATAATCAGTATCTTAAATACTCTTGGAAGCCTGATTCATGTGCTTTACCCAGGTATACTGTTAGTTTGTATTAATGTTCGTGTTTATTAAACCCTTTGTGATATGTCACATATAAAAAAAAATTTGTTTCTTACTATAGTGGTTGATCTTTGCTGCTAACAATTTCATGTTTCCATAGAAACAAAACACGTTTATTTAAGTCTATATATGTGACAATTAATATTTGGTAAATGTAACTAGTGTACTAAATTTCTTCTCCTAATGTATAGTACGAATGTAATGAGTAATTATTTTTGTGGTGTAGGTTCAGTGGAGAGGATTTTTTGAATAGGTGGAAGGATAAGAAGATAATGTTTGTAGGTGATTCATTGAGTCTAAACATGTGGGAATCATTGTCTTGTATGATTCATGCGTCGGTTCCTAATGTCACTACAAGCTTTTCTAGGAAAGAACCAATGTCTACTGTCTTCTTCCAGGTAATAGTTGTTCTTGTTTACTGTCTTAGACTCACACACATATGACACTATAGTGAGTGAATATATGTGTGTCATCATTTTGTTTGTTTCTTTGCGGGTTCTTTCTCTCTGTTACTAAAACTTGTTATATTCTGTTACTAAAACTTGTTACACGTAGTTTAAAAAAATAAAAAATAAAACCTGTTACATGTAAAATGATAGGTTTGTCGTTGATATGTAATTCAGAATTCGCCAAAGATATTAAGCTCGAAATTGAAGGTCTCGATATTGAGTTACTCCATCATTTTCCATAGAAATATTTGAATGAGACTAGGTTAGGCTGCCCGACAAAAGTTTATAGCTGATCTACATAAAGTCTGTTTCAATTAACTCGTTGAATAAAATGGTCAAGTTGAGACTCTTTAAAGAACTTATTTATTTAAATAGATCAAACTTAGACTATCTAAAAACCATATTATGCATGTCAAACCGACCTATATATAATTAAAATATAATATTATATATAATAATATTAAAGTGAGGTCAAGATTATCTTTAAAAAAGTGTGAAAAGTGAAAAAGTTACACACAAATAATAAAATGAAATAAAGCCTTATCACTAAATCTAGATATTTATTGACCTGTTAGCCTTTTTCAAAAGTCTTTACACAAAATAAGCTTTTAAAGAAGCTAATAGATTGGACCATAATTTTAAAAAGGTCAAGCCAAACTTAAAAAAAATAAGTCTTTAATATGTAATAGACTATAATCATGCTTCAATTTTTACAACTAAATCTAAAATTAACCTATTTAAACAAAGTCTGGACTCGACCTATTATCACCCTCAAACGAGAGGATGTCAACCTATGATTGCAACAGTTTACAAGTTGGAGGCTCTCTAATTTTATTTTTATTCTTTATTTTTGTGTCATCAACATCAATCATGGGTATGCTTTGGTTATTATTTATATCCTTATGTAAAGACAACTCTAAAGTTAACTATTTAATAACTAGTTTTTGTCGAATCTTTTCTTGAACAAATTTACTTTAGCAGTTTAATTTTTTCAAATTAAAATTTGTATTGTCATTATAGGGGACTCAAAGACTAACTACTTATTTCAGCTGAACATTTCTATGTTATGAATTTGCCCTTGCAACATATTTTTTTATCTTCCTTATTTAAAAATTTAAAACATATCAATTAATCATTAATTCATTATAATAGATGGATTAATTCGTCTTTTGTATGAAAATATCATAGACTACCTTCATATTTGATTTTTTTTTATAAGAAATTAGATTAAGCTGCAAGAAAATTGTGAAGACCCAAATTAAAGCTTTACTTTATTTCTATCCATATAGGGTGGATAAAAATATCCATATTCGTGGATATCCGGCCATTGCTATCCCCAATATGATAATATCAATGATATTATTATTCCTTCAAAAAAAAAAATCAATGATGTTATTATTAGTATTATTTGAAATTTAATTAATCTAAACCACCCATAATTAAACTGTGTTAGTGATACTTGGAGAGATTGCATGCACAGGACTATGGAGTAACCATACAACTATACCGTACACCATATTTGGTGGATATAATTCAAGAAGATGTTGGTAGAGTGCTTACGCTGGACTCTATAAAAGCTGGTAAAACATGGTTAGACATGGACATGTTGATCTTCAACTCCTGGCATTGGTGGATCCACACTGGAGATTTACGAGGGTATGTTTTAGTCTACAATAATACTATTTATTATGTAGTACCACAATTGAATGTATATTTGAATTGAGGTGATTTGAGGAAACTACGGTAGTAATACAGTTTTATTAAAATTATAAATTGTAAATTTTAGCAAAATTAGAGTGACACAGATTATGATTTTGTCAAACTTTCTGTTGATTTCACATCTGAACATGCTTTACACGTAAAGTGATGAATGTGTTATTTGGCTTGCTTCATTCAGGTGGGATTTTATCAGAGATGGTTCTAATCTAGTGAGGGACATGGACCGTTTGGATGCATTTTATAAGGGATTGACCACTTGGGCTGGTTGGGTTGATGCAAATGTTGACCCTACTAAAACTAAACTCTTCTTTCAAGGAATTTCCCCTACTCATAATAAGTAAGTAACTTCTAGTTATATGCTCATTTACACCAATACGTACTTTTAATTAGAGTGAAGAGCAAGTTTTCTTCCTTTCTCCTTGAATCCAGAGGGCAGGATTGGAATGAACCGAAGAAGACATGTAGCGGGGAAGTTGAACCATTACCGGGAACCACATATCCTGCCCCTCTACCTCCTGCAAATGATGTAGTGAACAAAGTGTTAAAGAACATGAAGAAGCAGGTTTATCTACTTGACATAACACTTCTCTCACAACTAAGAAAAGATGCACATCCTTCTATCTATACTAAGGATCCAACTGGCATTGATTGTAGTCATTGGTGCCTACCAGGACTACCTGATACTTGGAACATACTCCTATATGCAGCTCTTATCATGTGA
Protein sequence

>MS.gene008708.t1

MHPARAQKSNNCNLFFGSWVIDSSYPLYDSSSCPFVEAQFDCKKYGRPDNQYLKYSWKPDSCALPRFSGEDFLNRWKDKKIMFVGDSLSLNMWESLSCMIHASVPNVTTSFSRKEPMSTVFFQDYGVTIQLYRTPYLVDIIQEDVGRVLTLDSIKAGKTWLDMDMLIFNSWHWWIHTGDLRGWDFIRDGSNLVRDMDRLDAFYKGLTTWAGWVDANVDPTKTKLFFQGISPTHNKGQDWNEPKKTCSGEVEPLPGTTYPAPLPPANDVVNKVLKNMKKQVYLLDITLLSQLRKDAHPSIYTKDPTGIDCSHWCLPGLPDTWNILLYAALIM