Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74243.t1 | XP_003592837.1 | 78.2 | 55 | 4 | 3 | 1 | 55 | 1 | 47 | 3.90E-10 | 73.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74243.t1 | G7IB02 | 78.2 | 55 | 4 | 3 | 1 | 55 | 1 | 47 | 2.8e-10 | 73.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene74243 | 0.845372 | 4.23E-59 | -1.69E-46 |
| MS.gene04915 | MS.gene74243 | 0.828632 | 8.07E-55 | -1.69E-46 |
| MS.gene04917 | MS.gene74243 | 0.828444 | 8.96E-55 | -1.69E-46 |
| MS.gene049382 | MS.gene74243 | 0.814683 | 1.36E-51 | -1.69E-46 |
| MS.gene04984 | MS.gene74243 | 0.819926 | 8.99E-53 | -1.69E-46 |
| MS.gene050762 | MS.gene74243 | 0.842353 | 2.72E-58 | -1.69E-46 |
| MS.gene050763 | MS.gene74243 | 0.844451 | 7.50E-59 | -1.69E-46 |
| MS.gene050807 | MS.gene74243 | 0.804653 | 1.95E-49 | -1.69E-46 |
| MS.gene050826 | MS.gene74243 | 0.830091 | 3.57E-55 | -1.69E-46 |
| MS.gene051198 | MS.gene74243 | 0.810263 | 1.26E-50 | -1.69E-46 |
| MS.gene051210 | MS.gene74243 | 0.818146 | 2.28E-52 | -1.69E-46 |
| MS.gene052397 | MS.gene74243 | 0.801089 | 1.06E-48 | -1.69E-46 |
| MS.gene052665 | MS.gene74243 | 0.836353 | 9.83E-57 | -1.69E-46 |
| MS.gene052896 | MS.gene74243 | 0.840274 | 9.60E-58 | -1.69E-46 |
| MS.gene054379 | MS.gene74243 | 0.848394 | 6.30E-60 | -1.69E-46 |
| MS.gene054823 | MS.gene74243 | 0.81589 | 7.33E-52 | -1.69E-46 |
| MS.gene054930 | MS.gene74243 | 0.813271 | 2.79E-51 | -1.69E-46 |
| MS.gene055926 | MS.gene74243 | 0.807453 | 5.02E-50 | -1.69E-46 |
| MS.gene056480 | MS.gene74243 | 0.819571 | 1.08E-52 | -1.69E-46 |
| MS.gene057869 | MS.gene74243 | 0.819873 | 9.24E-53 | -1.69E-46 |
| MS.gene058646 | MS.gene74243 | 0.813434 | 2.56E-51 | -1.69E-46 |
| MS.gene059128 | MS.gene74243 | 0.806146 | 9.48E-50 | -1.69E-46 |
| MS.gene059713 | MS.gene74243 | 0.837035 | 6.59E-57 | -1.69E-46 |
| MS.gene060322 | MS.gene74243 | 0.80883 | 2.55E-50 | -1.69E-46 |
| MS.gene060480 | MS.gene74243 | 0.814841 | 1.25E-51 | -1.69E-46 |
| MS.gene06052 | MS.gene74243 | 0.855673 | 5.39E-62 | -1.69E-46 |
| MS.gene060677 | MS.gene74243 | 0.802918 | 4.47E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene74243 | 0.828068 | 1.10E-54 | -1.69E-46 |
| MS.gene060685 | MS.gene74243 | 0.817247 | 3.64E-52 | -1.69E-46 |
| MS.gene060802 | MS.gene74243 | 0.827435 | 1.57E-54 | -1.69E-46 |
| MS.gene060803 | MS.gene74243 | 0.804512 | 2.08E-49 | -1.69E-46 |
| MS.gene060998 | MS.gene74243 | 0.809002 | 2.35E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74243.t1 | MTR_1g116590 | 75.439 | 57 | 6 | 3 | 1 | 57 | 1 | 49 | 7.10e-19 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATGTTTGGATCTTGGTTT+TGG | 0.184629 | 1.2:-86541993 | MS.gene74243:CDS |
| TTGAAGGGATGTTTGGATCT+TGG | 0.331426 | 1.2:-86541999 | MS.gene74243:CDS |
| GATGAGTTTGAAGGGATGTT+TGG | 0.378887 | 1.2:-86542006 | MS.gene74243:CDS |
| ATGGAAGGTGGAGGTGTTGT+TGG | 0.413737 | 1.2:-86542156 | MS.gene74243:CDS |
| CATCAAGATCCTCATCTGTT+AGG | 0.424926 | 1.2:+86542025 | None:intergenic |
| GAGGATCTTGATGAGTTTGA+AGG | 0.470177 | 1.2:-86542015 | MS.gene74243:CDS |
| GGAGGTGTTGTTGGAAGAAG+TGG | 0.473169 | 1.2:-86542147 | MS.gene74243:CDS |
| TAACAAAGGAGTAGTGTTGT+TGG | 0.483852 | 1.2:-86542094 | MS.gene74243:CDS |
| ATAATGAGGAGGATCTTTGT+AGG | 0.495986 | 1.2:-86542056 | MS.gene74243:CDS |
| AAGGAGTAGTGTTGTTGGAA+GGG | 0.511274 | 1.2:-86542089 | MS.gene74243:CDS |
| AAAGGAGTAGTGTTGTTGGA+AGG | 0.535317 | 1.2:-86542090 | MS.gene74243:CDS |
| AGGATCTTGATGAGTTTGAA+GGG | 0.560777 | 1.2:-86542014 | MS.gene74243:CDS |
| AGGGTATGTTGATGATAATG+AGG | 0.584846 | 1.2:-86542070 | MS.gene74243:CDS |
| GTTGTTGGAAGAAGTGGAAG+TGG | 0.596897 | 1.2:-86542141 | MS.gene74243:CDS |
| GACGAAAAGCCTAACAGATG+AGG | 0.616832 | 1.2:-86542034 | MS.gene74243:CDS |
| TTGTTGGAAGAAGTGGAAGT+GGG | 0.619030 | 1.2:-86542140 | MS.gene74243:CDS |
| AGTAAGAATAACAATAACAA+AGG | 0.625105 | 1.2:-86542108 | MS.gene74243:CDS |
| GTATGTTGATGATAATGAGG+AGG | 0.675262 | 1.2:-86542067 | MS.gene74243:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAAGAATAACAATAACAA+AGG | - | chr1.2:86542029-86542048 | MS.gene74243:CDS | 20.0% |
| AGGATCTTGATGAGTTTGAA+GGG | - | chr1.2:86542123-86542142 | MS.gene74243:CDS | 35.0% | |
| ATAATGAGGAGGATCTTTGT+AGG | - | chr1.2:86542081-86542100 | MS.gene74243:CDS | 35.0% | |
| TAACAAAGGAGTAGTGTTGT+TGG | - | chr1.2:86542043-86542062 | MS.gene74243:CDS | 35.0% | |
| !! | AGGGTATGTTGATGATAATG+AGG | - | chr1.2:86542067-86542086 | MS.gene74243:CDS | 35.0% |
| !! | GATGTTTGGATCTTGGTTTT+GGG | - | chr1.2:86542145-86542164 | MS.gene74243:CDS | 35.0% |
| !! | GTATGTTGATGATAATGAGG+AGG | - | chr1.2:86542070-86542089 | MS.gene74243:CDS | 35.0% |
| CATCAAGATCCTCATCTGTT+AGG | + | chr1.2:86542115-86542134 | None:intergenic | 40.0% | |
| GAGGATCTTGATGAGTTTGA+AGG | - | chr1.2:86542122-86542141 | MS.gene74243:CDS | 40.0% | |
| GATGAGTTTGAAGGGATGTT+TGG | - | chr1.2:86542131-86542150 | MS.gene74243:CDS | 40.0% | |
| TTGAAGGGATGTTTGGATCT+TGG | - | chr1.2:86542138-86542157 | MS.gene74243:CDS | 40.0% | |
| TTGTTGGAAGAAGTGGAAGT+GGG | - | chr1.2:86541997-86542016 | MS.gene74243:CDS | 40.0% | |
| ! | AAAGGAGTAGTGTTGTTGGA+AGG | - | chr1.2:86542047-86542066 | MS.gene74243:CDS | 40.0% |
| ! | AAGGAGTAGTGTTGTTGGAA+GGG | - | chr1.2:86542048-86542067 | MS.gene74243:CDS | 40.0% |
| ! | GGATGTTTGGATCTTGGTTT+TGG | - | chr1.2:86542144-86542163 | MS.gene74243:CDS | 40.0% |
| GACGAAAAGCCTAACAGATG+AGG | - | chr1.2:86542103-86542122 | MS.gene74243:CDS | 45.0% | |
| GTTGTTGGAAGAAGTGGAAG+TGG | - | chr1.2:86541996-86542015 | MS.gene74243:CDS | 45.0% | |
| !! | ATGGAAGGTGGAGGTGTTGT+TGG | - | chr1.2:86541981-86542000 | MS.gene74243:CDS | 50.0% |
| !! | GGAGGTGTTGTTGGAAGAAG+TGG | - | chr1.2:86541990-86542009 | MS.gene74243:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 86541981 | 86542178 | 86541981 | ID=MS.gene74243 |
| chr1.2 | mRNA | 86541981 | 86542178 | 86541981 | ID=MS.gene74243.t1;Parent=MS.gene74243 |
| chr1.2 | exon | 86541981 | 86542178 | 86541981 | ID=MS.gene74243.t1.exon1;Parent=MS.gene74243.t1 |
| chr1.2 | CDS | 86541981 | 86542178 | 86541981 | ID=cds.MS.gene74243.t1;Parent=MS.gene74243.t1 |
| Gene Sequence |
| Protein sequence |
