Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74948.t1 | XP_013470311.1 | 96.2 | 105 | 2 | 1 | 1 | 103 | 1 | 105 | 6.80E-49 | 203 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74948.t1 | Q9SKC9 | 71.8 | 103 | 29 | 0 | 1 | 103 | 1 | 103 | 1.3e-35 | 150.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74948.t1 | A0A072VQV0 | 96.2 | 105 | 2 | 1 | 1 | 103 | 1 | 105 | 4.9e-49 | 203.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050838 | MS.gene74948 | 0.822554 | 2.23E-53 | -1.69E-46 |
| MS.gene051033 | MS.gene74948 | -0.809511 | 1.83E-50 | -1.69E-46 |
| MS.gene051063 | MS.gene74948 | 0.808308 | 3.30E-50 | -1.69E-46 |
| MS.gene051687 | MS.gene74948 | 0.834204 | 3.43E-56 | -1.69E-46 |
| MS.gene051746 | MS.gene74948 | 0.825841 | 3.76E-54 | -1.69E-46 |
| MS.gene051748 | MS.gene74948 | 0.803307 | 3.71E-49 | -1.69E-46 |
| MS.gene051756 | MS.gene74948 | 0.809807 | 1.58E-50 | -1.69E-46 |
| MS.gene051903 | MS.gene74948 | 0.846532 | 2.05E-59 | -1.69E-46 |
| MS.gene052947 | MS.gene74948 | 0.803613 | 3.21E-49 | -1.69E-46 |
| MS.gene054250 | MS.gene74948 | 0.805161 | 1.53E-49 | -1.69E-46 |
| MS.gene055555 | MS.gene74948 | 0.807444 | 5.04E-50 | -1.69E-46 |
| MS.gene056075 | MS.gene74948 | 0.825174 | 5.41E-54 | -1.69E-46 |
| MS.gene058906 | MS.gene74948 | 0.819477 | 1.14E-52 | -1.69E-46 |
| MS.gene059227 | MS.gene74948 | 0.811326 | 7.40E-51 | -1.69E-46 |
| MS.gene06001 | MS.gene74948 | 0.8144 | 1.57E-51 | -1.69E-46 |
| MS.gene06118 | MS.gene74948 | 0.81684 | 4.49E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74948.t1 | MTR_1g112480 | 96.190 | 105 | 2 | 1 | 1 | 103 | 1 | 105 | 8.09e-71 | 206 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74948.t1 | AT2G02050 | 71.845 | 103 | 29 | 0 | 1 | 103 | 1 | 103 | 2.49e-50 | 154 |
Find 25 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATCAAGCATTGGCGGGTT+TGG | 0.229519 | 1.3:+76316249 | None:intergenic |
| GATCAAGCATTGGCGGGTTT+GGG | 0.236660 | 1.3:+76316250 | None:intergenic |
| ACCCTCTGAAACGGGTTGTT+TGG | 0.368427 | 1.3:+76316291 | None:intergenic |
| CGTTCGTTCTCGCACTTCCA+TGG | 0.425940 | 1.3:+76316400 | None:intergenic |
| AAGAGGAGATGGTGGAAGCT+AGG | 0.454716 | 1.3:-76316501 | MS.gene74948:CDS |
| ATAGCAGCACCCTCTGAAAC+GGG | 0.480710 | 1.3:+76316283 | None:intergenic |
| AATAGCAGCACCCTCTGAAA+CGG | 0.485923 | 1.3:+76316282 | None:intergenic |
| CTTGTCTGCATTTGTTGAGA+GGG | 0.492622 | 1.3:+76316437 | None:intergenic |
| GGTGGAAGCTAGGGTTCCAT+TGG | 0.505790 | 1.3:-76316491 | MS.gene74948:CDS |
| GCTTGTCTGCATTTGTTGAG+AGG | 0.517488 | 1.3:+76316436 | None:intergenic |
| AAGCTGAATTCTATCTTCCA+TGG | 0.530156 | 1.3:-76316417 | MS.gene74948:CDS |
| AGAGGAGATGGTGGAAGCTA+GGG | 0.534962 | 1.3:-76316500 | MS.gene74948:CDS |
| ATCAAGCATTGGCGGGTTTG+GGG | 0.544039 | 1.3:+76316251 | None:intergenic |
| GAGAGGGATCAAGCATTGGC+GGG | 0.545254 | 1.3:+76316244 | None:intergenic |
| GTGCGAATACGAACTCGTCA+TGG | 0.547428 | 1.3:-76316362 | MS.gene74948:CDS |
| AGAGAGGGATCAAGCATTGG+CGG | 0.549265 | 1.3:+76316243 | None:intergenic |
| TCCAAACAACCCGTTTCAGA+GGG | 0.555884 | 1.3:-76316292 | MS.gene74948:CDS |
| GAATCGAAGATGGAATTTGA+AGG | 0.557443 | 1.3:-76316550 | None:intergenic |
| GAGAGAGAGGGATCAAGCAT+TGG | 0.561121 | 1.3:+76316240 | None:intergenic |
| TTCCAAACAACCCGTTTCAG+AGG | 0.569899 | 1.3:-76316293 | MS.gene74948:CDS |
| TTGGCGGGTTTGGGGATGAG+AGG | 0.579099 | 1.3:+76316259 | None:intergenic |
| GATTGCGACACAAGAGGAGA+TGG | 0.580463 | 1.3:-76316512 | MS.gene74948:CDS |
| CATTGATCTCTGTAAGCCAA+TGG | 0.586159 | 1.3:+76316475 | None:intergenic |
| TGCGACACAAGAGGAGATGG+TGG | 0.590865 | 1.3:-76316509 | MS.gene74948:CDS |
| GAAGATGATTGCGACACAAG+AGG | 0.593830 | 1.3:-76316518 | MS.gene74948:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTAGCATTTTGATTTTCG+CGG | + | chr1.3:76316482-76316501 | None:intergenic | 25.0% |
| AAGCTGAATTCTATCTTCCA+TGG | - | chr1.3:76316376-76316395 | MS.gene74948:CDS | 35.0% | |
| CATTGATCTCTGTAAGCCAA+TGG | + | chr1.3:76316321-76316340 | None:intergenic | 40.0% | |
| ! | CTTGTCTGCATTTGTTGAGA+GGG | + | chr1.3:76316359-76316378 | None:intergenic | 40.0% |
| GAAGATGATTGCGACACAAG+AGG | - | chr1.3:76316275-76316294 | MS.gene74948:CDS | 45.0% | |
| TCCAAACAACCCGTTTCAGA+GGG | - | chr1.3:76316501-76316520 | MS.gene74948:CDS | 45.0% | |
| TTCCAAACAACCCGTTTCAG+AGG | - | chr1.3:76316500-76316519 | MS.gene74948:CDS | 45.0% | |
| ! | AATAGCAGCACCCTCTGAAA+CGG | + | chr1.3:76316514-76316533 | None:intergenic | 45.0% |
| ! | GCTTGTCTGCATTTGTTGAG+AGG | + | chr1.3:76316360-76316379 | None:intergenic | 45.0% |
| GATTGCGACACAAGAGGAGA+TGG | - | chr1.3:76316281-76316300 | MS.gene74948:CDS | 50.0% | |
| GTGCGAATACGAACTCGTCA+TGG | - | chr1.3:76316431-76316450 | MS.gene74948:CDS | 50.0% | |
| !! | AAGAGGAGATGGTGGAAGCT+AGG | - | chr1.3:76316292-76316311 | MS.gene74948:CDS | 50.0% |
| !! | ACCCTCTGAAACGGGTTGTT+TGG | + | chr1.3:76316505-76316524 | None:intergenic | 50.0% |
| !! | AGAGGAGATGGTGGAAGCTA+GGG | - | chr1.3:76316293-76316312 | MS.gene74948:CDS | 50.0% |
| !! | ATAGCAGCACCCTCTGAAAC+GGG | + | chr1.3:76316513-76316532 | None:intergenic | 50.0% |
| CGTTCGTTCTCGCACTTCCA+TGG | + | chr1.3:76316396-76316415 | None:intergenic | 55.0% | |
| GGTGGAAGCTAGGGTTCCAT+TGG | - | chr1.3:76316302-76316321 | MS.gene74948:CDS | 55.0% | |
| TGCGACACAAGAGGAGATGG+TGG | - | chr1.3:76316284-76316303 | MS.gene74948:CDS | 55.0% | |
| !! | TTGGCGGGTTTGGGGATGAG+AGG | + | chr1.3:76316537-76316556 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 76316252 | 76316563 | 76316252 | ID=MS.gene74948 |
| chr1.3 | mRNA | 76316252 | 76316563 | 76316252 | ID=MS.gene74948.t1;Parent=MS.gene74948 |
| chr1.3 | exon | 76316252 | 76316563 | 76316252 | ID=MS.gene74948.t1.exon1;Parent=MS.gene74948.t1 |
| chr1.3 | CDS | 76316252 | 76316563 | 76316252 | ID=cds.MS.gene74948.t1;Parent=MS.gene74948.t1 |
| Gene Sequence |
| Protein sequence |
