AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75156

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75156.t1 MTR_1g115405 96.429 252 9 0 1 252 1 252 2.19e-174 480
MS.gene75156.t1 MTR_7g023350 86.905 252 33 0 1 252 1 252 8.11e-151 421
MS.gene75156.t1 MTR_4g063090 74.206 252 65 0 1 252 1 252 1.18e-126 359
MS.gene75156.t1 MTR_7g103030 66.667 252 82 2 1 252 1 250 2.23e-106 308
MS.gene75156.t1 MTR_1g077540 54.264 258 112 1 1 252 1 258 2.63e-95 280
MS.gene75156.t1 MTR_2g101370 58.300 247 101 2 6 252 5 249 2.28e-92 272
MS.gene75156.t1 MTR_8g013680 56.680 247 105 2 6 252 4 248 7.85e-91 268
MS.gene75156.t1 MTR_6g069040 56.078 255 109 1 1 252 1 255 3.40e-90 267
MS.gene75156.t1 MTR_1g006490 52.263 243 111 3 5 247 3 240 7.10e-82 246
MS.gene75156.t1 MTR_5g012810 54.032 248 111 2 5 252 3 247 2.66e-81 244
MS.gene75156.t1 MTR_1g006590 49.798 247 119 3 5 251 3 244 4.60e-78 236
MS.gene75156.t1 MTR_2g060360 39.583 240 138 2 12 251 10 242 3.98e-55 177
MS.gene75156.t1 MTR_2g094270 39.919 248 124 7 13 240 32 274 1.71e-40 141
MS.gene75156.t1 MTR_7g101190 40.000 230 122 6 21 235 39 267 1.51e-37 133
MS.gene75156.t1 MTR_3g118010 38.723 235 118 7 21 238 37 262 6.92e-35 126
MS.gene75156.t1 MTR_4g059390 36.290 248 134 7 12 240 30 272 2.89e-33 122
MS.gene75156.t1 MTR_5g063930 37.238 239 129 7 16 246 32 257 1.03e-31 117
MS.gene75156.t1 MTR_4g415300 37.295 244 126 8 13 239 48 281 1.76e-31 117
MS.gene75156.t1 MTR_5g010150 35.246 244 138 7 10 239 41 278 1.67e-30 115
MS.gene75156.t1 MTR_8g098375 34.167 240 139 6 10 235 41 275 2.37e-30 114
MS.gene75156.t1 MTR_1g095070 42.541 181 92 4 69 238 25 204 3.55e-30 112
MS.gene75156.t1 MTR_2g094270 38.785 214 109 6 13 209 32 240 7.21e-29 110
MS.gene75156.t1 MTR_3g070210 34.016 244 141 7 10 239 44 281 2.49e-28 109
MS.gene75156.t1 MTR_5g082070 34.979 243 131 7 10 234 44 277 3.71e-28 108
MS.gene75156.t1 MTR_3g008920 36.530 219 125 6 20 235 25 232 6.25e-24 97.1
MS.gene75156.t1 MTR_4g084880 32.906 234 148 3 20 251 34 260 6.80e-23 94.7
MS.gene75156.t1 MTR_1g097840 32.895 228 133 7 25 246 78 291 1.45e-22 94.4
MS.gene75156.t1 MTR_5g079570 32.877 219 133 3 20 235 39 246 1.64e-22 93.6
MS.gene75156.t1 MTR_2g017620 32.479 234 145 6 20 250 23 246 3.80e-22 92.4
MS.gene75156.t1 MTR_7g010650 31.897 232 140 5 21 248 37 254 5.94e-22 92.0
MS.gene75156.t1 MTR_2g017620 32.766 235 143 7 20 250 37 260 6.35e-22 92.0
MS.gene75156.t1 MTR_2g017620 34.286 175 111 3 78 251 9 180 9.69e-21 87.0
MS.gene75156.t1 MTR_2g104940 28.634 227 154 3 25 251 84 302 2.01e-20 88.6
MS.gene75156.t1 MTR_8g087710 32.420 219 139 5 20 237 38 248 2.18e-20 87.8
MS.gene75156.t1 MTR_1g095070 39.877 163 78 5 69 216 25 182 3.10e-20 85.9
MS.gene75156.t1 MTR_4g006730 29.358 218 146 3 25 242 79 288 4.09e-20 87.8
MS.gene75156.t1 MTR_2g017590 31.466 232 150 5 20 250 35 258 8.47e-20 86.3
MS.gene75156.t1 MTR_2g017660 30.734 218 144 3 20 237 38 248 2.64e-19 85.1
MS.gene75156.t1 MTR_4g006730 28.708 209 141 3 34 242 1 201 3.98e-18 80.5
MS.gene75156.t1 MTR_3g070210 31.222 221 133 6 10 216 44 259 3.98e-18 81.6
MS.gene75156.t1 MTR_4g059390 33.918 171 96 5 21 179 39 204 6.79e-18 79.7
MS.gene75156.t1 MTR_4g006630 28.708 209 141 3 34 242 1 201 7.44e-18 79.7
MS.gene75156.t1 MTR_8g087720 30.638 235 150 6 20 251 37 261 2.55e-17 79.3
MS.gene75156.t1 MTR_4g415300 34.463 177 97 5 13 179 48 215 6.23e-17 77.8
MS.gene75156.t1 MTR_2g017610 33.019 212 127 7 20 227 37 237 2.40e-16 76.6
MS.gene75156.t1 MTR_2g017570 32.547 212 128 7 20 227 37 237 8.68e-16 75.1
MS.gene75156.t1 MTR_4g075570 32.124 193 101 8 69 239 96 280 1.15e-15 75.5
MS.gene75156.t1 MTR_5g010150 31.638 177 109 4 10 179 41 212 1.95e-15 73.2
MS.gene75156.t1 MTR_8g098375 31.073 177 110 4 10 179 41 212 3.22e-15 73.2
MS.gene75156.t1 MTR_1g070385 29.268 164 113 2 79 242 115 275 3.29e-14 72.4
MS.gene75156.t1 MTR_3g070210 31.176 170 97 5 10 168 44 204 8.65e-14 68.6
MS.gene75156.t1 MTR_4g075590 27.309 249 150 9 12 239 28 266 1.68e-13 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75156.t1 AT4G01470 79.762 252 51 0 1 252 1 252 2.92e-144 404
MS.gene75156.t1 AT2G36830 69.444 252 76 1 1 252 1 251 1.82e-121 346
MS.gene75156.t1 AT3G26520 68.379 253 79 1 1 252 1 253 2.09e-118 338
MS.gene75156.t1 AT3G16240 54.656 247 112 0 6 252 4 250 1.10e-92 273
MS.gene75156.t1 AT1G73190 56.031 257 100 2 9 252 12 268 4.02e-90 267
MS.gene75156.t1 AT1G17810 54.297 256 105 2 9 252 12 267 2.30e-87 260
MS.gene75156.t1 AT5G47450 53.586 237 110 0 5 241 3 239 4.42e-86 256
MS.gene75156.t1 AT4G17340 52.439 246 117 0 5 250 3 248 6.15e-80 241
MS.gene75156.t1 AT2G25810 50.593 253 115 3 3 249 1 249 1.25e-76 232
MS.gene75156.t1 AT3G47440 41.150 226 130 2 19 242 21 245 6.79e-52 169
MS.gene75156.t1 AT3G54820 38.528 231 124 7 21 235 38 266 1.87e-36 130
MS.gene75156.t1 AT3G53420 39.565 230 123 6 21 235 39 267 8.45e-36 129
MS.gene75156.t1 AT3G53420 39.565 230 123 6 21 235 39 267 8.45e-36 129
MS.gene75156.t1 AT2G37170 37.395 238 133 6 13 235 29 265 3.60e-35 127
MS.gene75156.t1 AT2G37170 36.975 238 134 6 13 235 72 308 6.12e-35 127
MS.gene75156.t1 AT2G37180 36.820 239 135 6 12 235 28 265 2.61e-34 125
MS.gene75156.t1 AT5G60660 37.768 233 129 6 21 238 39 270 7.87e-34 124
MS.gene75156.t1 AT4G35100 37.872 235 120 7 21 238 38 263 7.24e-33 121
MS.gene75156.t1 AT4G35100 37.872 235 120 7 21 238 38 263 7.24e-33 121
MS.gene75156.t1 AT2G39010 37.551 245 125 8 12 235 29 266 8.10e-33 121
MS.gene75156.t1 AT2G16850 38.723 235 118 8 21 238 36 261 1.38e-32 120
MS.gene75156.t1 AT4G00430 36.032 247 131 8 10 239 42 278 1.95e-30 115
MS.gene75156.t1 AT4G23400 36.207 232 129 7 21 239 53 278 1.47e-29 112
MS.gene75156.t1 AT1G01620 35.246 244 131 8 13 239 44 277 9.71e-29 110
MS.gene75156.t1 AT2G45960 34.711 242 132 7 10 234 41 273 2.44e-28 109
MS.gene75156.t1 AT3G61430 35.146 239 129 7 13 234 44 273 2.76e-28 109
MS.gene75156.t1 AT3G61430 35.146 239 129 7 13 234 44 273 2.76e-28 109
MS.gene75156.t1 AT1G01620 38.071 197 99 7 60 239 15 205 2.89e-26 102
MS.gene75156.t1 AT5G37810 32.051 234 144 3 21 250 43 265 2.78e-22 93.2
MS.gene75156.t1 AT1G80760 29.536 237 149 5 21 252 80 303 3.77e-21 90.5
MS.gene75156.t1 AT5G37820 34.595 185 117 2 68 250 119 301 5.13e-21 90.5
MS.gene75156.t1 AT5G37820 34.595 185 117 2 68 250 83 265 6.43e-21 89.7
MS.gene75156.t1 AT4G18910 28.689 244 148 6 20 250 51 281 5.11e-20 87.4
MS.gene75156.t1 AT4G10380 30.088 226 150 4 21 246 78 295 1.06e-19 86.7
MS.gene75156.t1 AT4G19030 30.417 240 149 6 20 250 54 284 2.18e-19 85.5
MS.gene75156.t1 AT1G52180 55.556 72 32 0 145 216 53 124 7.06e-19 80.1
MS.gene75156.t1 AT2G39010 37.427 171 86 6 12 168 29 192 2.05e-18 81.6
MS.gene75156.t1 AT2G45960 32.143 224 126 7 10 216 41 255 4.44e-18 81.6
MS.gene75156.t1 AT2G45960 32.143 224 126 7 10 216 41 255 5.62e-18 81.6
MS.gene75156.t1 AT1G31885 31.004 229 130 7 20 234 42 256 3.88e-17 79.7
MS.gene75156.t1 AT3G06100 30.631 222 145 6 16 237 43 255 6.03e-17 78.6
MS.gene75156.t1 AT1G31885 34.807 181 99 6 67 234 28 202 3.45e-16 76.3
MS.gene75156.t1 AT3G06100 30.939 181 123 2 57 237 29 207 7.95e-16 74.7
MS.gene75156.t1 AT4G00430 33.728 169 93 5 10 168 42 201 5.49e-15 72.0
MS.gene75156.t1 AT3G06100 33.083 133 87 2 105 237 44 174 2.74e-11 61.6
MS.gene75156.t1 AT2G34390 26.446 242 146 7 12 235 34 261 8.59e-11 61.2

Find 70 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGTTGGTGCAAATATTTC+TGG 0.152046 1.1:-81131756 MS.gene75156:CDS
TCTAGAAGTCACTATTTAAA+AGG 0.160161 1.1:+81130696 None:intergenic
GGACTCATCATTGGGTCTAT+TGG 0.245534 1.1:-81130796 MS.gene75156:CDS
GCATCATTATCTCATGCATT+TGG 0.272168 1.1:-81131795 MS.gene75156:CDS
ACACCAACATTCCCTCTCTT+TGG 0.279029 1.1:+81130938 None:intergenic
TATTGGGTTGGCCCATTCAT+TGG 0.290949 1.1:-81130779 MS.gene75156:CDS
GACTCATCATTGGGTCTATT+GGG 0.324641 1.1:-81130795 MS.gene75156:CDS
GTCACATTTGGTGCTTTCAT+TGG 0.337136 1.1:-81131714 MS.gene75156:CDS
CATCATTATCTCATGCATTT+GGG 0.337895 1.1:-81131794 MS.gene75156:CDS
TAGGTGCAAATATCTTGGTT+GGG 0.348151 1.1:-81130886 MS.gene75156:CDS
ATGAACCCAGCAGTGTCATT+TGG 0.350767 1.1:-81130842 MS.gene75156:CDS
CTAGAAGTCACTATTTAAAA+GGG 0.362303 1.1:+81130697 None:intergenic
TTTGAGATTGTGATGACATT+TGG 0.368730 1.1:-81130995 MS.gene75156:CDS
CACCAACATTCCCTCTCTTT+GGG 0.379452 1.1:+81130939 None:intergenic
TTAGCAATTGGTTGTGTTGT+AGG 0.382904 1.1:-81130905 MS.gene75156:CDS
GATGCAACTATGAGACCTTC+AGG 0.383026 1.1:+81131813 None:intergenic
TATTGGATTGCACAGTTACT+TGG 0.390300 1.1:-81131660 MS.gene75156:CDS
TGAACCCAGCAGTGTCATTT+GGG 0.394408 1.1:-81130841 MS.gene75156:CDS
CTCTATGATAATATCTTCAT+AGG 0.401368 1.1:-81130737 MS.gene75156:CDS
ATTCTTCTCAATTCCTGCAC+TGG 0.411800 1.1:-81131621 MS.gene75156:CDS
TTTGGGCCTGCTGTGGTTAG+TGG 0.417445 1.1:-81130824 MS.gene75156:CDS
GTGGGACATGGACTCATCAT+TGG 0.427196 1.1:-81130805 MS.gene75156:CDS
TGTTGTAGGTGCAAATATCT+TGG 0.429108 1.1:-81130891 MS.gene75156:CDS
GCAATCTACAGAATAGCAAT+TGG 0.439083 1.1:-81132323 MS.gene75156:CDS
TCATGCATTTGGGTTATTTG+TGG 0.441279 1.1:-81131784 MS.gene75156:CDS
AGGTGCAAATATCTTGGTTG+GGG 0.447005 1.1:-81130885 MS.gene75156:CDS
CATCATTGGGTCTATTGGGT+TGG 0.461096 1.1:-81130791 MS.gene75156:CDS
GTAGGTGCAAATATCTTGGT+TGG 0.462763 1.1:-81130887 MS.gene75156:CDS
GCAGGATTGCTAGCCTCTCT+AGG 0.469123 1.1:+81132296 None:intergenic
ACATTTGGTGCTTTCATTGG+AGG 0.472714 1.1:-81131711 MS.gene75156:CDS
TGCTGGCCAAGGCTCCAGCA+TGG 0.476065 1.1:-81132222 MS.gene75156:CDS
CTGCTGTGGTTAGTGGGACA+TGG 0.476983 1.1:-81130817 MS.gene75156:CDS
ACAACACAACCAATTGCTAA+TGG 0.477910 1.1:+81130908 None:intergenic
CTTCTCAATTCCTGCACTGG+TGG 0.486622 1.1:-81131618 MS.gene75156:CDS
ATTTACCATTCCACCAGTGC+AGG 0.488777 1.1:+81131608 None:intergenic
GTGAACCCTGCAGTCACATT+TGG 0.490396 1.1:-81131726 MS.gene75156:CDS
GCAGTAGCTGAACCAATGAA+TGG 0.490483 1.1:+81130767 None:intergenic
TATAGGCCATGCTGGAGCCT+TGG 0.492628 1.1:+81132216 None:intergenic
CAGGATTGCTAGCCTCTCTA+GGG 0.497959 1.1:+81132297 None:intergenic
TTATTTGTGGCTGTTTCTGT+TGG 0.501056 1.1:-81131771 MS.gene75156:CDS
CAATTCCTGCACTGGTGGAA+TGG 0.501839 1.1:-81131613 MS.gene75156:intron
GCAGGCCCAAATGACACTGC+TGG 0.509169 1.1:+81130836 None:intergenic
GAAGATATTATCATAGAGAA+TGG 0.513994 1.1:+81130742 None:intergenic
TGGGACATGGACTCATCATT+GGG 0.515058 1.1:-81130804 MS.gene75156:CDS
GTTGTAGCACCATTAGCAAT+TGG 0.518540 1.1:-81130917 MS.gene75156:CDS
AAGGCTGCTCTAATTGCAGC+AGG 0.523234 1.1:+81132278 None:intergenic
AAAGCACCAAATGTGACTGC+AGG 0.524218 1.1:+81131720 None:intergenic
GATTGTGATGACATTTGGAT+TGG 0.530182 1.1:-81130990 MS.gene75156:CDS
TTACTCACTATAGGCCATGC+TGG 0.531666 1.1:+81132208 None:intergenic
CAGTAGCTGAACCAATGAAT+GGG 0.532725 1.1:+81130768 None:intergenic
TTGGGCCTGCTGTGGTTAGT+GGG 0.535621 1.1:-81130823 MS.gene75156:CDS
AATGGAGCTGCCACTCCTGA+AGG 0.566075 1.1:-81131828 MS.gene75156:CDS
GCAGATAAACTTACAAACAA+TGG 0.566186 1.1:-81131846 MS.gene75156:intron
ATACAAAATACTTCTCAAGA+AGG 0.568809 1.1:+81131680 None:intergenic
TATCCTCTGGTGTGTCTGTG+TGG 0.569335 1.1:-81131030 MS.gene75156:CDS
CATGTCCCACTAACCACAGC+AGG 0.579587 1.1:+81130818 None:intergenic
CAGGCCCAAATGACACTGCT+GGG 0.586045 1.1:+81130837 None:intergenic
TAGAAGTCACTATTTAAAAG+GGG 0.591596 1.1:+81130698 None:intergenic
GTTGGTGCAAATATTTCTGG+TGG 0.597567 1.1:-81131753 MS.gene75156:CDS
GACCCAAAGAGAGGGAATGT+TGG 0.602021 1.1:-81130941 MS.gene75156:CDS
AGTGTCATTTGGGCCTGCTG+TGG 0.612489 1.1:-81130831 MS.gene75156:CDS
CAGCAATAGACCCAAAGAGA+GGG 0.627157 1.1:-81130949 MS.gene75156:CDS
ACAGCAATAGACCCAAAGAG+AGG 0.635119 1.1:-81130950 MS.gene75156:CDS
CATAGAGAAGAACTCTGCAA+AGG 0.637931 1.1:+81132259 None:intergenic
AAGCACCAAATGTGACTGCA+GGG 0.650741 1.1:+81131721 None:intergenic
AGCAATTGGATCCCCTAGAG+AGG 0.652271 1.1:-81132309 MS.gene75156:CDS
AACTATGAGACCTTCAGGAG+TGG 0.653111 1.1:+81131818 None:intergenic
GGTGCAAATATCTTGGTTGG+GGG 0.667124 1.1:-81130884 MS.gene75156:CDS
AGGATTGCTAGCCTCTCTAG+GGG 0.675172 1.1:+81132298 None:intergenic
ATTCCACACAGACACACCAG+AGG 0.744381 1.1:+81131027 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTGTAAAAAAAAATGTATA+TGG + chr1.1:81130873-81130892 None:intergenic 15.0%
!!! AAGTTTCTAAAAATTATAGA+AGG + chr1.1:81131052-81131071 None:intergenic 15.0%
!!! AGTTTCTAAAAATTATAGAA+GGG + chr1.1:81131051-81131070 None:intergenic 15.0%
!! ATATCTGGATTTAAAAATGA+AGG + chr1.1:81131534-81131553 None:intergenic 20.0%
!! CTTCACTTAATATAAAATAC+AGG - chr1.1:81131459-81131478 MS.gene75156:intron 20.0%
!!! AAAATTTGAAGATAGTTTGT+TGG - chr1.1:81131146-81131165 MS.gene75156:intron 20.0%
!!! AAGATCTAGTATTTCTAAAA+TGG + chr1.1:81130943-81130962 None:intergenic 20.0%
!!! TTATTGATTGATATTTGAGT+AGG - chr1.1:81130912-81130931 MS.gene75156:CDS 20.0%
! AAATTGGAAGACTAAATATC+TGG + chr1.1:81131549-81131568 None:intergenic 25.0%
! ATACAAAATACTTCTCAAGA+AGG + chr1.1:81131346-81131365 None:intergenic 25.0%
! CTAGAAGTCACTATTTAAAA+GGG + chr1.1:81132329-81132348 None:intergenic 25.0%
! CTCTATGATAATATCTTCAT+AGG - chr1.1:81132286-81132305 MS.gene75156:CDS 25.0%
! GAAGATATTATCATAGAGAA+TGG + chr1.1:81132284-81132303 None:intergenic 25.0%
! TAGAAGTCACTATTTAAAAG+GGG + chr1.1:81132328-81132347 None:intergenic 25.0%
! TATATAGAAGTAATTGAACG+TGG - chr1.1:81131813-81131832 MS.gene75156:CDS 25.0%
! TCAATAAAAAGATAGAGTGT+TGG - chr1.1:81130996-81131015 MS.gene75156:CDS 25.0%
! TTATCTAGATTACTCACTAT+AGG + chr1.1:81130827-81130846 None:intergenic 25.0%
!! ACAACGTTAATTTAAAGTGT+CGG - chr1.1:81131875-81131894 MS.gene75156:intron 25.0%
!! TTGTACTTCGTTTAATTTAG+AGG + chr1.1:81131504-81131523 None:intergenic 25.0%
!! TTGTTGACATTTTCTACATA+TGG - chr1.1:81131948-81131967 MS.gene75156:intron 25.0%
!!! AAACCAGAAACTTTTGAAAT+TGG + chr1.1:81131565-81131584 None:intergenic 25.0%
!!! ATCTAGTATTTCTAAAATGG+TGG + chr1.1:81130940-81130959 None:intergenic 25.0%
!!! TCTAGTATTTCTAAAATGGT+GGG + chr1.1:81130939-81130958 None:intergenic 25.0%
!!! TCTTGAGAAGTATTTTGTAT+TGG - chr1.1:81131346-81131365 MS.gene75156:intron 25.0%
AAATTAAACCCAAATGTGAG+CGG + chr1.1:81131596-81131615 None:intergenic 30.0%
CATCATTATCTCATGCATTT+GGG - chr1.1:81131229-81131248 MS.gene75156:intron 30.0%
CTTCCAATTTCAAAAGTTTC+TGG - chr1.1:81131559-81131578 MS.gene75156:intron 30.0%
GCAGATAAACTTACAAACAA+TGG - chr1.1:81131177-81131196 MS.gene75156:intron 30.0%
GTTTCAATGTCAGTAATTAG+AGG + chr1.1:81131630-81131649 None:intergenic 30.0%
! TGATAATGTTGAAATGGAGT+GGG - chr1.1:81131663-81131682 MS.gene75156:CDS 30.0%
! TTTGAGATTGTGATGACATT+TGG - chr1.1:81132028-81132047 MS.gene75156:intron 30.0%
!! CTAGTATTTCTAAAATGGTG+GGG + chr1.1:81130938-81130957 None:intergenic 30.0%
!!! ATGCTCATTTTTGTTTTTGC+TGG - chr1.1:81130784-81130803 MS.gene75156:CDS 30.0%
ACAACACAACCAATTGCTAA+TGG + chr1.1:81132118-81132137 None:intergenic 35.0%
CGTTGTGTTCAATGTCTTTA+TGG + chr1.1:81131861-81131880 None:intergenic 35.0%
GATTGTGATGACATTTGGAT+TGG - chr1.1:81132033-81132052 MS.gene75156:intron 35.0%
GCAATCTACAGAATAGCAAT+TGG - chr1.1:81130700-81130719 MS.gene75156:CDS 35.0%
GCATCATTATCTCATGCATT+TGG - chr1.1:81131228-81131247 MS.gene75156:intron 35.0%
TAGGTGCAAATATCTTGGTT+GGG - chr1.1:81132137-81132156 MS.gene75156:intron 35.0%
TATTGGATTGCACAGTTACT+TGG - chr1.1:81131363-81131382 MS.gene75156:intron 35.0%
TCTGTTGGTGCAAATATTTC+TGG - chr1.1:81131267-81131286 MS.gene75156:intron 35.0%
TGTTGTAGGTGCAAATATCT+TGG - chr1.1:81132132-81132151 MS.gene75156:intron 35.0%
TTAGCAATTGGTTGTGTTGT+AGG - chr1.1:81132118-81132137 MS.gene75156:intron 35.0%
! ACGACGTGATAATGTTGAAA+TGG - chr1.1:81131657-81131676 MS.gene75156:CDS 35.0%
! GTGATAATGTTGAAATGGAG+TGG - chr1.1:81131662-81131681 MS.gene75156:CDS 35.0%
! TCATGCATTTGGGTTATTTG+TGG - chr1.1:81131239-81131258 MS.gene75156:intron 35.0%
! TGACATTTTCTACATATGGC+AGG - chr1.1:81131952-81131971 MS.gene75156:intron 35.0%
!! AGATAGTTTGTTGGCTATTG+TGG - chr1.1:81131155-81131174 MS.gene75156:intron 35.0%
!! TTATTTGTGGCTGTTTCTGT+TGG - chr1.1:81131252-81131271 MS.gene75156:intron 35.0%
!!! ATTTTTGTTTTTGCTGGCCA+AGG - chr1.1:81130790-81130809 MS.gene75156:CDS 35.0%
AGGTGCAAATATCTTGGTTG+GGG - chr1.1:81132138-81132157 MS.gene75156:intron 40.0%
ATTCTTCTCAATTCCTGCAC+TGG - chr1.1:81131402-81131421 MS.gene75156:intron 40.0%
CAGTAGCTGAACCAATGAAT+GGG + chr1.1:81132258-81132277 None:intergenic 40.0%
CATAGAGAAGAACTCTGCAA+AGG + chr1.1:81130767-81130786 None:intergenic 40.0%
GACTCATCATTGGGTCTATT+GGG - chr1.1:81132228-81132247 MS.gene75156:CDS 40.0%
GTAGGTGCAAATATCTTGGT+TGG - chr1.1:81132136-81132155 MS.gene75156:intron 40.0%
GTGTATGTGTCCAATACATG+TGG + chr1.1:81131839-81131858 None:intergenic 40.0%
GTTGTAGCACCATTAGCAAT+TGG - chr1.1:81132106-81132125 MS.gene75156:intron 40.0%
TCTATTCACCGCTCACATTT+GGG - chr1.1:81131585-81131604 MS.gene75156:intron 40.0%
TTCTATTCACCGCTCACATT+TGG - chr1.1:81131584-81131603 MS.gene75156:intron 40.0%
!! ACATTTGGTGCTTTCATTGG+AGG - chr1.1:81131312-81131331 MS.gene75156:intron 40.0%
!! GTCACATTTGGTGCTTTCAT+TGG - chr1.1:81131309-81131328 MS.gene75156:intron 40.0%
!! GTTGGTGCAAATATTTCTGG+TGG - chr1.1:81131270-81131289 MS.gene75156:intron 40.0%
AACTATGAGACCTTCAGGAG+TGG + chr1.1:81131208-81131227 None:intergenic 45.0%
AAGCACCAAATGTGACTGCA+GGG + chr1.1:81131305-81131324 None:intergenic 45.0%
ACACCAACATTCCCTCTCTT+TGG + chr1.1:81132088-81132107 None:intergenic 45.0%
ACAGCAATAGACCCAAAGAG+AGG - chr1.1:81132073-81132092 MS.gene75156:intron 45.0%
ATTTACCATTCCACCAGTGC+AGG + chr1.1:81131418-81131437 None:intergenic 45.0%
CACCAACATTCCCTCTCTTT+GGG + chr1.1:81132087-81132106 None:intergenic 45.0%
CAGCAATAGACCCAAAGAGA+GGG - chr1.1:81132074-81132093 MS.gene75156:intron 45.0%
CATCATTGGGTCTATTGGGT+TGG - chr1.1:81132232-81132251 MS.gene75156:CDS 45.0%
GATGCAACTATGAGACCTTC+AGG + chr1.1:81131213-81131232 None:intergenic 45.0%
GCAGTAGCTGAACCAATGAA+TGG + chr1.1:81132259-81132278 None:intergenic 45.0%
GGACTCATCATTGGGTCTAT+TGG - chr1.1:81132227-81132246 MS.gene75156:CDS 45.0%
GGTGCAAATATCTTGGTTGG+GGG - chr1.1:81132139-81132158 MS.gene75156:intron 45.0%
TGGGACATGGACTCATCATT+GGG - chr1.1:81132219-81132238 MS.gene75156:CDS 45.0%
TTACTCACTATAGGCCATGC+TGG + chr1.1:81130818-81130837 None:intergenic 45.0%
TTGAACGTGGCCACATGTAT+TGG - chr1.1:81131826-81131845 MS.gene75156:CDS 45.0%
! AAAGCACCAAATGTGACTGC+AGG + chr1.1:81131306-81131325 None:intergenic 45.0%
! ATGAACCCAGCAGTGTCATT+TGG - chr1.1:81132181-81132200 MS.gene75156:intron 45.0%
! TCAGCTTTTGCTCTATCCTC+TGG - chr1.1:81131980-81131999 MS.gene75156:intron 45.0%
!! TATTGGGTTGGCCCATTCAT+TGG - chr1.1:81132244-81132263 MS.gene75156:CDS 45.0%
!! TGAACCCAGCAGTGTCATTT+GGG - chr1.1:81132182-81132201 MS.gene75156:intron 45.0%
!!! TTGGTTGGGGGTGTTTTTGA+TGG - chr1.1:81132151-81132170 MS.gene75156:intron 45.0%
AAGGCTGCTCTAATTGCAGC+AGG + chr1.1:81130748-81130767 None:intergenic 50.0%
AGCAATTGGATCCCCTAGAG+AGG - chr1.1:81130714-81130733 MS.gene75156:CDS 50.0%
ATTCCACACAGACACACCAG+AGG + chr1.1:81131999-81132018 None:intergenic 50.0%
CTTCTCAATTCCTGCACTGG+TGG - chr1.1:81131405-81131424 MS.gene75156:intron 50.0%
GACCCAAAGAGAGGGAATGT+TGG - chr1.1:81132082-81132101 MS.gene75156:intron 50.0%
GTGAACCCTGCAGTCACATT+TGG - chr1.1:81131297-81131316 MS.gene75156:intron 50.0%
GTGGGACATGGACTCATCAT+TGG - chr1.1:81132218-81132237 MS.gene75156:CDS 50.0%
TATCCTCTGGTGTGTCTGTG+TGG - chr1.1:81131993-81132012 MS.gene75156:intron 50.0%
! AGGATTGCTAGCCTCTCTAG+GGG + chr1.1:81130728-81130747 None:intergenic 50.0%
! CAATTCCTGCACTGGTGGAA+TGG - chr1.1:81131410-81131429 MS.gene75156:intron 50.0%
! CAGGATTGCTAGCCTCTCTA+GGG + chr1.1:81130729-81130748 None:intergenic 50.0%
AATGGAGCTGCCACTCCTGA+AGG - chr1.1:81131195-81131214 MS.gene75156:intron 55.0%
CAGGCCCAAATGACACTGCT+GGG + chr1.1:81132189-81132208 None:intergenic 55.0%
CATGTCCCACTAACCACAGC+AGG + chr1.1:81132208-81132227 None:intergenic 55.0%
CTGCTGTGGTTAGTGGGACA+TGG - chr1.1:81132206-81132225 MS.gene75156:intron 55.0%
TATAGGCCATGCTGGAGCCT+TGG + chr1.1:81130810-81130829 None:intergenic 55.0%
TTGGGCCTGCTGTGGTTAGT+GGG - chr1.1:81132200-81132219 MS.gene75156:intron 55.0%
TTTGGGCCTGCTGTGGTTAG+TGG - chr1.1:81132199-81132218 MS.gene75156:intron 55.0%
! GCAGGATTGCTAGCCTCTCT+AGG + chr1.1:81130730-81130749 None:intergenic 55.0%
!! AGTGTCATTTGGGCCTGCTG+TGG - chr1.1:81132192-81132211 MS.gene75156:intron 55.0%
GCAGGCCCAAATGACACTGC+TGG + chr1.1:81132190-81132209 None:intergenic 60.0%
TGCTGGCCAAGGCTCCAGCA+TGG - chr1.1:81130801-81130820 MS.gene75156:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 81130697 81132348 81130697 ID=MS.gene75156
chr1.1 mRNA 81130697 81132348 81130697 ID=MS.gene75156.t1;Parent=MS.gene75156
chr1.1 exon 81132216 81132348 81132216 ID=MS.gene75156.t1.exon1;Parent=MS.gene75156.t1
chr1.1 CDS 81132216 81132348 81132216 ID=cds.MS.gene75156.t1;Parent=MS.gene75156.t1
chr1.1 exon 81131614 81131864 81131614 ID=MS.gene75156.t1.exon2;Parent=MS.gene75156.t1
chr1.1 CDS 81131614 81131864 81131614 ID=cds.MS.gene75156.t1;Parent=MS.gene75156.t1
chr1.1 exon 81130697 81131071 81130697 ID=MS.gene75156.t1.exon3;Parent=MS.gene75156.t1
chr1.1 CDS 81130697 81131071 81130697 ID=cds.MS.gene75156.t1;Parent=MS.gene75156.t1
Gene Sequence

>MS.gene75156

ATGGCAATCTACAGAATAGCAATTGGATCCCCTAGAGAGGCTAGCAATCCTGCTGCAATTAGAGCAGCCTTTGCAGAGTTCTTCTCTATGCTCATTTTTGTTTTTGCTGGCCAAGGCTCCAGCATGGCCTATAGTGAGTAATCTAGATAATCTCTTGAAGAGTATTACATATTCCATATACATTTTTTTTTACACTCTTTTTTATACTCGTTCAATTATTGATTGATATTTGAGTAGGCCCCACCATTTTAGAAATACTAGATCTTAGATAAAGTGACATAGATGTACGATAATTTCACTCAATAAAAAGATAGAGTGTTGGAAACGAATTATTAGTTAGTTTATTGTAAGCCCTTCTATAATTTTTAGAAACTTATTTTGTGACATTAAAAGATAAAATTGCAGTTAGAATTTTTTTTCTTATTATTTTTCAATGCTTAGCATTGTTGAAAATTTGAAGATAGTTTGTTGGCTATTGTGGCAGATAAACTTACAAACAATGGAGCTGCCACTCCTGAAGGTCTCATAGTTGCATCATTATCTCATGCATTTGGGTTATTTGTGGCTGTTTCTGTTGGTGCAAATATTTCTGGTGGACATGTGAACCCTGCAGTCACATTTGGTGCTTTCATTGGAGGAAACATTACCTTCTTGAGAAGTATTTTGTATTGGATTGCACAGTTACTTGGCGCAGTTGTTGCTTGCATTCTTCTCAATTCCTGCACTGGTGGAATGGTAAATAAATAAATTTGCACATGCATTCTTCACTTAATATAAAATACAGGTTCAAGTGTACTTTTTGTGCCTCTAAATTAAACGAAGTACAATTTTAATCCTTCATTTTTAAATCCAGATATTTAGTCTTCCAATTTCAAAAGTTTCTGGTTTTCTATTCACCGCTCACATTTGGGTTTAATTTTGTTGATGTGTCCTCTAATTACTGACATTGAAACACATTTGACGACGTGATAATGTTGAAATGGAGTGGGTTTAATTTTATCTGTATTCACATTTGTGTTTTATTAACTATAAAACACAAACTTGATCAAAAAAAGAAACTATAAAACACAAAAATCACACGCGACATTGACATGCTAATACATATAAAAATTTGAATATATAGAAGTAATTGAACGTGGCCACATGTATTGGACACATACACCATAAAGACATTGAACACAACGTTAATTTAAAGTGTCGGTGTTACATAGATTATTCGCTCGCATTTAATGATTGAATTTGAATATAATTTTGTTGACATTTTCTACATATGGCAGGAAACATCAGCTTTTGCTCTATCCTCTGGTGTGTCTGTGTGGAATGCATTAGTTTTTGAGATTGTGATGACATTTGGATTGGTATATACAGTTTATGCAACAGCAATAGACCCAAAGAGAGGGAATGTTGGTGTTGTAGCACCATTAGCAATTGGTTGTGTTGTAGGTGCAAATATCTTGGTTGGGGGTGTTTTTGATGGTGCATCAATGAACCCAGCAGTGTCATTTGGGCCTGCTGTGGTTAGTGGGACATGGACTCATCATTGGGTCTATTGGGTTGGCCCATTCATTGGTTCAGCTACTGCTGCCATTCTCTATGATAATATCTTCATAGGTGATGATGCTCATGAACCCCTTTTAAATAGTGACTTCTAG
Protein sequence

>MS.gene75156.t1

MAIYRIAIGSPREASNPAAIRAAFAEFFSMLIFVFAGQGSSMAYNKLTNNGAATPEGLIVASLSHAFGLFVAVSVGANISGGHVNPAVTFGAFIGGNITFLRSILYWIAQLLGAVVACILLNSCTGGMETSAFALSSGVSVWNALVFEIVMTFGLVYTVYATAIDPKRGNVGVVAPLAIGCVVGANILVGGVFDGASMNPAVSFGPAVVSGTWTHHWVYWVGPFIGSATAAILYDNIFIGDDAHEPLLNSDF