AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67362

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67362.t1 MTR_4g063090 99.603 252 1 0 1 252 1 252 4.40e-178 489
MS.gene67362.t1 MTR_7g023350 77.778 252 56 0 1 252 1 252 2.44e-131 371
MS.gene67362.t1 MTR_1g115405 73.810 252 66 0 1 252 1 252 6.82e-130 367
MS.gene67362.t1 MTR_7g103030 75.000 252 61 2 1 252 1 250 1.71e-120 343
MS.gene67362.t1 MTR_2g101370 64.876 242 84 1 6 247 5 245 2.97e-100 292
MS.gene67362.t1 MTR_8g013680 61.475 244 85 2 14 251 6 246 1.68e-97 285
MS.gene67362.t1 MTR_1g077540 52.713 258 116 1 1 252 1 258 9.44e-95 279
MS.gene67362.t1 MTR_6g069040 54.510 255 113 1 1 252 1 255 1.15e-89 266
MS.gene67362.t1 MTR_5g012810 59.274 248 98 2 5 252 3 247 1.01e-84 253
MS.gene67362.t1 MTR_1g006490 53.586 237 106 2 3 239 1 233 2.55e-84 252
MS.gene67362.t1 MTR_1g006590 50.612 245 117 2 3 247 1 241 1.47e-80 242
MS.gene67362.t1 MTR_2g060360 43.578 218 122 1 18 235 16 232 3.51e-57 183
MS.gene67362.t1 MTR_2g094270 36.066 244 131 6 12 235 31 269 2.00e-38 135
MS.gene67362.t1 MTR_7g101190 36.929 241 136 6 10 235 28 267 1.16e-36 131
MS.gene67362.t1 MTR_3g118010 36.555 238 136 6 21 247 37 270 1.50e-34 125
MS.gene67362.t1 MTR_8g098375 33.193 238 144 5 10 235 41 275 1.75e-31 117
MS.gene67362.t1 MTR_4g059390 33.745 243 137 6 12 235 30 267 1.88e-31 117
MS.gene67362.t1 MTR_5g063930 35.021 237 136 4 18 245 34 261 2.33e-30 114
MS.gene67362.t1 MTR_1g095070 40.722 194 94 6 69 247 25 212 6.36e-30 112
MS.gene67362.t1 MTR_3g070210 33.195 241 138 6 10 234 44 277 1.37e-29 112
MS.gene67362.t1 MTR_4g415300 34.513 226 134 5 21 235 56 278 1.58e-29 112
MS.gene67362.t1 MTR_5g010150 32.353 238 146 5 10 235 41 275 3.81e-29 111
MS.gene67362.t1 MTR_5g082070 32.911 237 144 5 10 234 44 277 1.36e-28 110
MS.gene67362.t1 MTR_2g094270 35.814 215 116 5 12 209 31 240 2.41e-28 108
MS.gene67362.t1 MTR_4g084880 32.751 229 147 4 20 247 34 256 2.02e-25 101
MS.gene67362.t1 MTR_2g017620 33.624 229 143 5 20 247 23 243 2.97e-25 100
MS.gene67362.t1 MTR_2g017620 33.624 229 143 5 20 247 37 257 3.48e-25 100
MS.gene67362.t1 MTR_2g017590 33.188 229 144 5 20 247 35 255 1.31e-23 96.7
MS.gene67362.t1 MTR_2g017620 36.842 171 104 3 78 247 9 176 3.40e-23 93.6
MS.gene67362.t1 MTR_3g008920 35.616 219 127 6 20 235 25 232 3.75e-23 95.1
MS.gene67362.t1 MTR_2g104940 33.498 203 127 4 25 227 84 278 4.61e-22 93.2
MS.gene67362.t1 MTR_5g079570 32.727 220 132 6 20 235 39 246 4.74e-22 92.4
MS.gene67362.t1 MTR_1g070385 28.972 214 144 4 21 234 62 267 5.57e-22 95.1
MS.gene67362.t1 MTR_8g087710 32.314 229 146 5 20 247 38 258 2.10e-21 90.5
MS.gene67362.t1 MTR_7g010650 31.356 236 146 6 21 250 37 262 4.43e-21 89.7
MS.gene67362.t1 MTR_1g095070 40.491 163 77 5 69 216 25 182 1.23e-20 86.7
MS.gene67362.t1 MTR_3g070210 31.416 226 132 6 10 219 44 262 1.33e-20 88.6
MS.gene67362.t1 MTR_2g017660 30.041 243 159 5 10 248 25 260 1.66e-20 88.2
MS.gene67362.t1 MTR_8g087720 32.129 249 151 7 8 251 21 256 3.36e-20 87.4
MS.gene67362.t1 MTR_4g006730 31.401 207 134 3 21 227 75 273 1.89e-19 85.9
MS.gene67362.t1 MTR_1g097840 32.719 217 124 8 25 234 78 279 8.29e-19 84.0
MS.gene67362.t1 MTR_2g017610 31.330 233 146 7 20 251 37 256 2.00e-18 82.4
MS.gene67362.t1 MTR_2g017570 31.330 233 146 7 20 251 37 256 3.18e-18 82.0
MS.gene67362.t1 MTR_4g059390 31.667 180 106 4 12 179 30 204 4.59e-18 80.1
MS.gene67362.t1 MTR_8g098375 30.337 178 116 3 10 182 41 215 6.07e-17 77.8
MS.gene67362.t1 MTR_4g006730 30.412 194 127 3 34 227 1 186 8.13e-17 77.0
MS.gene67362.t1 MTR_4g006630 30.412 194 127 3 34 227 1 186 8.38e-17 77.0
MS.gene67362.t1 MTR_4g415300 33.136 169 94 5 21 179 56 215 1.69e-16 76.3
MS.gene67362.t1 MTR_3g070210 30.899 178 107 4 10 178 44 214 5.28e-16 74.7
MS.gene67362.t1 MTR_5g010150 30.286 175 114 3 10 179 41 212 1.32e-15 73.9
MS.gene67362.t1 MTR_4g075570 27.072 181 110 4 69 229 96 274 8.57e-13 67.4
MS.gene67362.t1 MTR_4g075590 26.582 237 149 6 13 229 29 260 1.12e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67362.t1 AT2G36830 80.556 252 48 1 1 252 1 251 3.21e-143 401
MS.gene67362.t1 AT4G01470 77.778 252 56 0 1 252 1 252 1.32e-139 392
MS.gene67362.t1 AT3G26520 76.285 253 59 1 1 252 1 253 8.71e-134 377
MS.gene67362.t1 AT5G47450 60.324 247 96 1 5 251 3 247 1.71e-95 280
MS.gene67362.t1 AT3G16240 58.000 250 105 0 3 252 1 250 3.00e-94 277
MS.gene67362.t1 AT1G73190 58.333 240 94 2 9 243 12 250 1.22e-90 269
MS.gene67362.t1 AT1G17810 57.851 242 96 2 9 245 12 252 2.16e-88 263
MS.gene67362.t1 AT4G17340 59.514 247 98 1 5 251 3 247 9.39e-86 256
MS.gene67362.t1 AT2G25810 53.012 249 110 3 3 251 1 242 1.11e-79 240
MS.gene67362.t1 AT3G47440 42.922 219 124 1 18 236 20 237 6.88e-53 172
MS.gene67362.t1 AT2G37170 36.822 258 140 8 11 247 21 276 4.01e-36 129
MS.gene67362.t1 AT2G37170 36.822 258 140 8 11 247 64 319 6.37e-36 130
MS.gene67362.t1 AT3G53420 37.450 251 140 7 12 247 30 278 7.52e-36 129
MS.gene67362.t1 AT3G53420 37.450 251 140 7 12 247 30 278 7.52e-36 129
MS.gene67362.t1 AT3G54820 34.855 241 141 5 10 235 27 266 7.41e-35 126
MS.gene67362.t1 AT2G37180 35.938 256 141 8 10 247 26 276 3.02e-34 125
MS.gene67362.t1 AT2G39010 35.102 245 131 7 12 235 29 266 4.73e-34 124
MS.gene67362.t1 AT4G35100 36.134 238 137 6 21 247 38 271 8.47e-34 123
MS.gene67362.t1 AT4G35100 36.134 238 137 6 21 247 38 271 8.47e-34 123
MS.gene67362.t1 AT5G60660 35.060 251 146 7 12 247 30 278 1.16e-33 123
MS.gene67362.t1 AT2G16850 35.294 238 139 6 21 247 36 269 2.98e-32 119
MS.gene67362.t1 AT4G00430 33.745 243 135 7 10 235 42 275 1.15e-29 113
MS.gene67362.t1 AT4G23400 34.052 232 127 6 21 235 53 275 7.79e-29 110
MS.gene67362.t1 AT3G61430 35.065 231 124 7 21 234 52 273 5.06e-28 108
MS.gene67362.t1 AT3G61430 35.065 231 124 7 21 234 52 273 5.06e-28 108
MS.gene67362.t1 AT1G01620 34.052 232 127 7 21 235 52 274 5.39e-28 108
MS.gene67362.t1 AT2G45960 33.884 242 134 7 10 234 41 273 1.12e-27 107
MS.gene67362.t1 AT1G01620 37.500 184 91 6 69 235 26 202 1.33e-25 100
MS.gene67362.t1 AT1G52180 56.627 83 36 0 134 216 42 124 2.07e-23 92.0
MS.gene67362.t1 AT1G80760 32.579 221 140 5 25 245 84 295 1.45e-22 94.4
MS.gene67362.t1 AT4G19030 29.804 255 157 6 8 247 34 281 4.11e-21 90.1
MS.gene67362.t1 AT5G37810 30.837 227 150 3 21 247 43 262 9.77e-21 89.0
MS.gene67362.t1 AT5G37820 29.961 257 161 5 3 247 13 262 2.18e-20 88.2
MS.gene67362.t1 AT5G37820 29.961 257 161 5 3 247 49 298 3.66e-20 88.2
MS.gene67362.t1 AT2G39010 33.149 181 100 5 12 178 29 202 9.47e-20 85.1
MS.gene67362.t1 AT4G18910 28.814 236 152 6 20 247 51 278 9.75e-19 84.0
MS.gene67362.t1 AT2G45960 32.143 224 126 7 10 216 41 255 1.27e-18 83.2
MS.gene67362.t1 AT2G45960 32.143 224 126 7 10 216 41 255 1.44e-18 83.6
MS.gene67362.t1 AT3G06100 27.273 220 153 4 18 237 43 255 1.81e-18 82.8
MS.gene67362.t1 AT1G31885 29.237 236 151 4 20 247 42 269 2.23e-18 83.2
MS.gene67362.t1 AT4G10380 31.163 215 130 5 25 234 82 283 8.25e-18 81.3
MS.gene67362.t1 AT1G31885 33.146 178 108 3 78 247 41 215 1.46e-17 80.1
MS.gene67362.t1 AT3G06100 27.700 213 147 4 24 236 1 206 3.18e-17 78.6
MS.gene67362.t1 AT4G00430 32.022 178 102 5 10 177 42 210 3.28e-15 72.8
MS.gene67362.t1 AT3G06100 31.061 132 89 2 105 236 44 173 1.81e-13 67.4

Find 64 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGTTGGTGCTAACATTTC+TGG 0.108507 4.1:-37330638 MS.gene67362:CDS
ATGCTTATCTTTGTCTTTGC+AGG 0.219702 4.1:-37330924 MS.gene67362:CDS
GGGCTAATCATTGGGTCTAT+TGG 0.263332 4.1:-37329691 MS.gene67362:CDS
ATGAACCCAGCAGTTTCTTT+TGG 0.263396 4.1:-37329737 MS.gene67362:CDS
GTTAACCCTGCTGTCACTTT+TGG 0.284147 4.1:-37330608 MS.gene67362:CDS
ATTGGAAATCCTTCTGAGTT+TGG 0.293211 4.1:-37330990 MS.gene67362:CDS
TTTGAGATTGTGATGACTTT+TGG 0.309198 4.1:-37329890 MS.gene67362:CDS
TTACCGGTCACGGATTATTA+AGG 0.336094 4.1:-37329590 None:intergenic
ATTGCAATTGGTTTCATAGT+TGG 0.339661 4.1:-37329800 MS.gene67362:CDS
CTGCTGTTGTTAGCTGGACA+TGG 0.355517 4.1:-37329712 MS.gene67362:CDS
GTCTTTGCAGGGGAAGGCTC+TGG 0.356147 4.1:-37330912 MS.gene67362:CDS
AAAAGAAACTGCTGGGTTCA+TGG 0.357172 4.1:+37329739 None:intergenic
CCAATTGCAATTCTAGAAAT+CGG 0.358019 4.1:+37331008 None:intergenic
CCGATTTCTAGAATTGCAAT+TGG 0.370439 4.1:-37331008 MS.gene67362:CDS
TTGGGCCTGCTGTTGTTAGC+TGG 0.373980 4.1:-37329718 MS.gene67362:CDS
GGCTAATCATTGGGTCTATT+GGG 0.374997 4.1:-37329690 MS.gene67362:CDS
TGGCAAAGCTGATGCACTTA+AGG 0.381290 4.1:-37330970 MS.gene67362:CDS
ATCTTTGTCTTTGCAGGGGA+AGG 0.391790 4.1:-37330918 MS.gene67362:CDS
GCTGGACATGGGCTAATCAT+TGG 0.392902 4.1:-37329700 MS.gene67362:CDS
TCTGCATTCTCATTATCTTC+TGG 0.394809 4.1:-37329938 MS.gene67362:CDS
TATTGGATTGCTCAGTTGCT+TGG 0.424094 4.1:-37330542 MS.gene67362:CDS
TTAGCAGGTGGTGCATTTGA+TGG 0.429164 4.1:-37329767 MS.gene67362:CDS
ACAATTGCTCCAATTGCAAT+TGG 0.430105 4.1:-37329812 MS.gene67362:CDS
TAGCTCTTATGAACAGTTAC+CGG 0.430523 4.1:-37329606 MS.gene67362:CDS
GGACATATTACACTCATTAG+AGG 0.450565 4.1:-37330572 MS.gene67362:CDS
CAATTGGAGCAATTGTTCCT+AGG 0.454950 4.1:+37329819 None:intergenic
TTCTCATTATCTTCTGGTGT+GGG 0.456268 4.1:-37329932 MS.gene67362:CDS
ACTATGAAACCAATTGCAAT+TGG 0.458046 4.1:+37329803 None:intergenic
TATTGGGCCGGCCCATTGAT+CGG 0.462419 4.1:-37329674 MS.gene67362:CDS
GCAATAGCAGAACCGATCAA+TGG 0.473518 4.1:+37329662 None:intergenic
TGCTTATCTTTGTCTTTGCA+GGG 0.474084 4.1:-37330923 MS.gene67362:CDS
CTGGACATGGGCTAATCATT+GGG 0.474402 4.1:-37329699 MS.gene67362:CDS
CTCCTTAAAATTGCCACTGG+TGG 0.474415 4.1:-37330500 MS.gene67362:CDS
CAATAGCAGAACCGATCAAT+GGG 0.488984 4.1:+37329663 None:intergenic
AATGGTGCAGCAACACCAGC+AGG 0.497841 4.1:-37330710 MS.gene67362:CDS
GCAGGCCCAAAAGAAACTGC+TGG 0.500317 4.1:+37329731 None:intergenic
TAAAATTGCCACTGGTGGAC+TGG 0.504856 4.1:-37330495 MS.gene67362:intron
TGCAGGGGAAGGCTCTGGCA+TGG 0.516920 4.1:-37330907 MS.gene67362:CDS
AATCATTGGGTCTATTGGGC+CGG 0.520340 4.1:-37329686 MS.gene67362:CDS
GGTAACTGTTCATAAGAGCT+AGG 0.525678 4.1:+37329608 None:intergenic
TTGCTCCTTAAAATTGCCAC+TGG 0.528763 4.1:-37330503 MS.gene67362:CDS
CATGTCCAGCTAACAACAGC+AGG 0.530958 4.1:+37329713 None:intergenic
ACTGAGCAATCCAATACAAA+AGG 0.532220 4.1:+37330549 None:intergenic
GTAACTGTTCATAAGAGCTA+GGG 0.532887 4.1:+37329609 None:intergenic
GTTGGAGCCAACATCTTAGC+AGG 0.533340 4.1:-37329782 MS.gene67362:CDS
TGCTGTTGTTAGCTGGACAT+GGG 0.535749 4.1:-37329711 MS.gene67362:CDS
AATGCACCAAAAGTGACAGC+AGG 0.542618 4.1:+37330602 None:intergenic
ATTCTCATTATCTTCTGGTG+TGG 0.543635 4.1:-37329933 MS.gene67362:CDS
TTATGAACAGTTACCGGTCA+CGG 0.547163 4.1:-37329600 MS.gene67362:CDS
AGGCCTTAATAATCCGTGAC+CGG 0.557820 4.1:+37329587 None:intergenic
TGCACCACCTGCTAAGATGT+TGG 0.559448 4.1:+37329775 None:intergenic
AGCAGAACCGATCAATGGGC+CGG 0.562596 4.1:+37329667 None:intergenic
GATGCTGCTACCAACCCTGC+TGG 0.563647 4.1:+37330695 None:intergenic
TCAGCTTTGCCAAACTCAGA+AGG 0.564993 4.1:+37330981 None:intergenic
GTTGGTGCTAACATTTCTGG+TGG 0.567619 4.1:-37330635 MS.gene67362:CDS
ACAGATAAGCTTACAAACAA+TGG 0.573110 4.1:-37330728 MS.gene67362:intron
TGCAGCAACACCAGCAGGGT+TGG 0.574909 4.1:-37330705 MS.gene67362:CDS
CAGGCCCAAAAGAAACTGCT+GGG 0.603420 4.1:+37329732 None:intergenic
GTTTCTCACCAGTCCACCAG+TGG 0.659307 4.1:+37330487 None:intergenic
GCTTATCTTTGTCTTTGCAG+GGG 0.662008 4.1:-37330922 MS.gene67362:CDS
ATGCACCAAAAGTGACAGCA+GGG 0.675489 4.1:+37330603 None:intergenic
ATGGTGCAGCAACACCAGCA+GGG 0.686064 4.1:-37330709 MS.gene67362:CDS
TAACTGTTCATAAGAGCTAG+GGG 0.686347 4.1:+37329610 None:intergenic
GGAGCCAACATCTTAGCAGG+TGG 0.690619 4.1:-37329779 MS.gene67362:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGTTAAATCGAATTTTTT+TGG + chr4.1:37329760-37329779 None:intergenic 15.0%
!! ACTATAAGCATAAAACAATT+TGG + chr4.1:37330466-37330485 None:intergenic 20.0%
!! ATATTCGTTAATTTCTTTCA+AGG - chr4.1:37330439-37330458 MS.gene67362:intron 20.0%
!! ATCTAAATAGTCTAAACTAT+AGG - chr4.1:37330287-37330306 MS.gene67362:intron 20.0%
!! GAAAGTTAATTACAAAGATA+AGG + chr4.1:37330592-37330611 None:intergenic 20.0%
!!! ATTTGATCACATTGAAATTT+TGG + chr4.1:37330393-37330412 None:intergenic 20.0%
!!! TTGTAATTAACTTTCCTTTT+TGG - chr4.1:37330597-37330616 MS.gene67362:CDS 20.0%
!!! TTGTTTTATGCTTATAGTTT+TGG - chr4.1:37330468-37330487 MS.gene67362:intron 20.0%
! GTAATGAAATACCTTAACTA+TGG - chr4.1:37330322-37330341 MS.gene67362:intron 25.0%
!!! TTTATTTTCGACCATAGTTA+AGG + chr4.1:37330336-37330355 None:intergenic 25.0%
ACAGATAAGCTTACAAACAA+TGG - chr4.1:37329875-37329894 MS.gene67362:CDS 30.0%
ACTATGAAACCAATTGCAAT+TGG + chr4.1:37330803-37330822 None:intergenic 30.0%
CTAAATTGAATCCATTGTGT+CGG - chr4.1:37330161-37330180 MS.gene67362:intron 30.0%
TTACTTTGTGAACTCTTCTT+GGG - chr4.1:37329839-37329858 MS.gene67362:CDS 30.0%
! ATTGCAATTGGTTTCATAGT+TGG - chr4.1:37330803-37330822 MS.gene67362:intron 30.0%
! CCAATTGCAATTCTAGAAAT+CGG + chr4.1:37329598-37329617 None:intergenic 30.0%
!! TTTGAGATTGTGATGACTTT+TGG - chr4.1:37330713-37330732 MS.gene67362:CDS 30.0%
AATCTGTGTATGTCTATTGC+AGG - chr4.1:37330637-37330656 MS.gene67362:CDS 35.0%
ACAATTGCTCCAATTGCAAT+TGG - chr4.1:37330791-37330810 MS.gene67362:intron 35.0%
ACTAGAATTCAGCACCAAAA+AGG + chr4.1:37330614-37330633 None:intergenic 35.0%
ACTGAGCAATCCAATACAAA+AGG + chr4.1:37330057-37330076 None:intergenic 35.0%
ATGCTTATCTTTGTCTTTGC+AGG - chr4.1:37329679-37329698 MS.gene67362:CDS 35.0%
ATTCTCATTATCTTCTGGTG+TGG - chr4.1:37330670-37330689 MS.gene67362:CDS 35.0%
ATTGGAAATCCTTCTGAGTT+TGG - chr4.1:37329613-37329632 MS.gene67362:CDS 35.0%
CTTACTTTGTGAACTCTTCT+TGG - chr4.1:37329838-37329857 MS.gene67362:CDS 35.0%
GGACATATTACACTCATTAG+AGG - chr4.1:37330031-37330050 MS.gene67362:intron 35.0%
GTAACTGTTCATAAGAGCTA+GGG + chr4.1:37330997-37331016 None:intergenic 35.0%
TAACTGTTCATAAGAGCTAG+GGG + chr4.1:37330996-37331015 None:intergenic 35.0%
TACTTTGTGAACTCTTCTTG+GGG - chr4.1:37329840-37329859 MS.gene67362:CDS 35.0%
TAGCTCTTATGAACAGTTAC+CGG - chr4.1:37330997-37331016 MS.gene67362:CDS 35.0%
TCTGCATTCTCATTATCTTC+TGG - chr4.1:37330665-37330684 MS.gene67362:CDS 35.0%
TGCTTATCTTTGTCTTTGCA+GGG - chr4.1:37329680-37329699 MS.gene67362:CDS 35.0%
TTCTCATTATCTTCTGGTGT+GGG - chr4.1:37330671-37330690 MS.gene67362:CDS 35.0%
! TCATTAGAGGCCTTTTGTAT+TGG - chr4.1:37330044-37330063 MS.gene67362:intron 35.0%
!! CCGATTTCTAGAATTGCAAT+TGG - chr4.1:37329595-37329614 MS.gene67362:CDS 35.0%
!! GATTGTGATGACTTTTGGTT+TGG - chr4.1:37330718-37330737 MS.gene67362:CDS 35.0%
!!! CTTTTTGTTGCTGTTTCTGT+TGG - chr4.1:37329950-37329969 MS.gene67362:intron 35.0%
!!! TTCCTAGGCTACCATTTTTT+GGG + chr4.1:37330772-37330791 None:intergenic 35.0%
AAAAGAAACTGCTGGGTTCA+TGG + chr4.1:37330867-37330886 None:intergenic 40.0%
ACTGCAGTAGACCCAAAAAA+TGG - chr4.1:37330758-37330777 MS.gene67362:intron 40.0%
ATGGTAACATGACTTTCCCT+CGG - chr4.1:37330189-37330208 MS.gene67362:intron 40.0%
CAATAGCAGAACCGATCAAT+GGG + chr4.1:37330943-37330962 None:intergenic 40.0%
CAATTGGAGCAATTGTTCCT+AGG + chr4.1:37330787-37330806 None:intergenic 40.0%
CTCGAGGATTTGTCTCATAA+CGG - chr4.1:37330264-37330283 MS.gene67362:intron 40.0%
GCTTATCTTTGTCTTTGCAG+GGG - chr4.1:37329681-37329700 MS.gene67362:CDS 40.0%
GGCTAATCATTGGGTCTATT+GGG - chr4.1:37330913-37330932 MS.gene67362:CDS 40.0%
GGTAACTGTTCATAAGAGCT+AGG + chr4.1:37330998-37331017 None:intergenic 40.0%
TAAATGGACGACTTCAGGAA+TGG + chr4.1:37330231-37330250 None:intergenic 40.0%
TATTGGATTGCTCAGTTGCT+TGG - chr4.1:37330061-37330080 MS.gene67362:intron 40.0%
TTATGAACAGTTACCGGTCA+CGG - chr4.1:37331003-37331022 MS.gene67362:CDS 40.0%
TTGCTCCTTAAAATTGCCAC+TGG - chr4.1:37330100-37330119 MS.gene67362:intron 40.0%
! ATGAACCCAGCAGTTTCTTT+TGG - chr4.1:37330866-37330885 MS.gene67362:intron 40.0%
! GAGTTGATCTGCCAACTAAA+TGG + chr4.1:37330247-37330266 None:intergenic 40.0%
! TGAACCCAGCAGTTTCTTTT+GGG - chr4.1:37330867-37330886 MS.gene67362:intron 40.0%
!! ACTTTTGGTGCATTCATTGG+TGG - chr4.1:37330010-37330029 MS.gene67362:intron 40.0%
!! TCTGTTGGTGCTAACATTTC+TGG - chr4.1:37329965-37329984 MS.gene67362:intron 40.0%
!!! GTCACTTTTGGTGCATTCAT+TGG - chr4.1:37330007-37330026 MS.gene67362:intron 40.0%
!!! GTTCCTAGGCTACCATTTTT+TGG + chr4.1:37330773-37330792 None:intergenic 40.0%
AATCATTGGGTCTATTGGGC+CGG - chr4.1:37330917-37330936 MS.gene67362:CDS 45.0%
AATGCACCAAAAGTGACAGC+AGG + chr4.1:37330004-37330023 None:intergenic 45.0%
ACGACTTCAGGAATGGAACT+CGG + chr4.1:37330224-37330243 None:intergenic 45.0%
ATCCATTGTGTCGGAAGTGA+TGG - chr4.1:37330170-37330189 MS.gene67362:intron 45.0%
ATCTTTGTCTTTGCAGGGGA+AGG - chr4.1:37329685-37329704 MS.gene67362:CDS 45.0%
ATGCACCAAAAGTGACAGCA+GGG + chr4.1:37330003-37330022 None:intergenic 45.0%
CCAACTAAATGGACGACTTC+AGG + chr4.1:37330236-37330255 None:intergenic 45.0%
CCTGAAGTCGTCCATTTAGT+TGG - chr4.1:37330233-37330252 MS.gene67362:intron 45.0%
CTCCTTAAAATTGCCACTGG+TGG - chr4.1:37330103-37330122 MS.gene67362:intron 45.0%
CTCTGGCATGGCATATAGTA+AGG - chr4.1:37329708-37329727 MS.gene67362:CDS 45.0%
GACCCAAAAAATGGTAGCCT+AGG - chr4.1:37330767-37330786 MS.gene67362:intron 45.0%
GCAATAGCAGAACCGATCAA+TGG + chr4.1:37330944-37330963 None:intergenic 45.0%
GGGCTAATCATTGGGTCTAT+TGG - chr4.1:37330912-37330931 MS.gene67362:CDS 45.0%
TACCATCACTTCCGACACAA+TGG + chr4.1:37330175-37330194 None:intergenic 45.0%
TCAGCTTTGCCAAACTCAGA+AGG + chr4.1:37329625-37329644 None:intergenic 45.0%
TGCTGTTGTTAGCTGGACAT+GGG - chr4.1:37330892-37330911 MS.gene67362:intron 45.0%
TGGCAAAGCTGATGCACTTA+AGG - chr4.1:37329633-37329652 MS.gene67362:CDS 45.0%
TGGTAACATGACTTTCCCTC+GGG - chr4.1:37330190-37330209 MS.gene67362:intron 45.0%
TTAGCAGGTGGTGCATTTGA+TGG - chr4.1:37330836-37330855 MS.gene67362:intron 45.0%
TTAGTTGGCAGATCAACTCG+AGG - chr4.1:37330248-37330267 MS.gene67362:intron 45.0%
! CTGGACATGGGCTAATCATT+GGG - chr4.1:37330904-37330923 MS.gene67362:CDS 45.0%
! GTCCACCAGTGGCAATTTTA+AGG + chr4.1:37330108-37330127 None:intergenic 45.0%
! TAAAATTGCCACTGGTGGAC+TGG - chr4.1:37330108-37330127 MS.gene67362:intron 45.0%
!! GTTGGTGCTAACATTTCTGG+TGG - chr4.1:37329968-37329987 MS.gene67362:intron 45.0%
!!! GTTAACCCTGCTGTCACTTT+TGG - chr4.1:37329995-37330014 MS.gene67362:intron 45.0%
CAGGCCCAAAAGAAACTGCT+GGG + chr4.1:37330874-37330893 None:intergenic 50.0%
CATGTCCAGCTAACAACAGC+AGG + chr4.1:37330893-37330912 None:intergenic 50.0%
CGACTTCAGGAATGGAACTC+GGG + chr4.1:37330223-37330242 None:intergenic 50.0%
CTGCTGTTGTTAGCTGGACA+TGG - chr4.1:37330891-37330910 MS.gene67362:intron 50.0%
GTTGGAGCCAACATCTTAGC+AGG - chr4.1:37330821-37330840 MS.gene67362:intron 50.0%
TGCACCACCTGCTAAGATGT+TGG + chr4.1:37330831-37330850 None:intergenic 50.0%
! AACTCGGGTTCTTTTCCCGA+GGG + chr4.1:37330208-37330227 None:intergenic 50.0%
! GCTGGACATGGGCTAATCAT+TGG - chr4.1:37330903-37330922 MS.gene67362:CDS 50.0%
AATGGTGCAGCAACACCAGC+AGG - chr4.1:37329893-37329912 MS.gene67362:CDS 55.0%
AGCAGAACCGATCAATGGGC+CGG + chr4.1:37330939-37330958 None:intergenic 55.0%
ATGGTGCAGCAACACCAGCA+GGG - chr4.1:37329894-37329913 MS.gene67362:CDS 55.0%
GCAGGCCCAAAAGAAACTGC+TGG + chr4.1:37330875-37330894 None:intergenic 55.0%
GGAGCCAACATCTTAGCAGG+TGG - chr4.1:37330824-37330843 MS.gene67362:intron 55.0%
GTTTCTCACCAGTCCACCAG+TGG + chr4.1:37330119-37330138 None:intergenic 55.0%
TATTGGGCCGGCCCATTGAT+CGG - chr4.1:37330929-37330948 MS.gene67362:CDS 55.0%
TTGGGCCTGCTGTTGTTAGC+TGG - chr4.1:37330885-37330904 MS.gene67362:intron 55.0%
! GAACTCGGGTTCTTTTCCCG+AGG + chr4.1:37330209-37330228 None:intergenic 55.0%
GATGCTGCTACCAACCCTGC+TGG + chr4.1:37329911-37329930 None:intergenic 60.0%
GTCTTTGCAGGGGAAGGCTC+TGG - chr4.1:37329691-37329710 MS.gene67362:CDS 60.0%
TGCAGCAACACCAGCAGGGT+TGG - chr4.1:37329898-37329917 MS.gene67362:CDS 60.0%
! TGCAGGGGAAGGCTCTGGCA+TGG - chr4.1:37329696-37329715 MS.gene67362:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 37329592 37331033 37329592 ID=MS.gene67362
chr4.1 mRNA 37329592 37331033 37329592 ID=MS.gene67362.t1;Parent=MS.gene67362
chr4.1 exon 37330901 37331033 37330901 ID=MS.gene67362.t1.exon1;Parent=MS.gene67362.t1
chr4.1 CDS 37330901 37331033 37330901 ID=cds.MS.gene67362.t1;Parent=MS.gene67362.t1
chr4.1 exon 37330496 37330746 37330496 ID=MS.gene67362.t1.exon2;Parent=MS.gene67362.t1
chr4.1 CDS 37330496 37330746 37330496 ID=cds.MS.gene67362.t1;Parent=MS.gene67362.t1
chr4.1 exon 37329592 37329966 37329592 ID=MS.gene67362.t1.exon3;Parent=MS.gene67362.t1
chr4.1 CDS 37329592 37329966 37329592 ID=cds.MS.gene67362.t1;Parent=MS.gene67362.t1
Gene Sequence

>MS.gene67362

ATGCCGATTTCTAGAATTGCAATTGGAAATCCTTCTGAGTTTGGCAAAGCTGATGCACTTAAGGCTGCACTAGCTGAGTTCATTTCAATGCTTATCTTTGTCTTTGCAGGGGAAGGCTCTGGCATGGCATATAGTAAGGCTTTTTATTAGTTAATTAAACTCTTTCCAAAAAAATTCGATTTAACTTATTTGAGTTTATCTACTGACATAAACATTTGTGTAACTATTTCAAAAAACTGTTTTCAACTTACTTTGTGAACTCTTCTTGGGGTGTGTTTTTTTGACAGATAAGCTTACAAACAATGGTGCAGCAACACCAGCAGGGTTGGTAGCAGCATCACTATCACATGCATTTGCACTTTTTGTTGCTGTTTCTGTTGGTGCTAACATTTCTGGTGGACATGTTAACCCTGCTGTCACTTTTGGTGCATTCATTGGTGGACATATTACACTCATTAGAGGCCTTTTGTATTGGATTGCTCAGTTGCTTGGTTCTGTTGTTGCTTGTTTGCTCCTTAAAATTGCCACTGGTGGACTGGTGAGAAACATTACATATATATTTATTCATTCTAAATTGAATCCATTGTGTCGGAAGTGATGGTAACATGACTTTCCCTCGGGAAAAGAACCCGAGTTCCATTCCTGAAGTCGTCCATTTAGTTGGCAGATCAACTCGAGGATTTGTCTCATAACGGATCTAAATAGTCTAAACTATAGGATAGAATCTATTGTAATGAAATACCTTAACTATGGTCGAAAATAAAAAAAATTCAATCACATTAAGACTAAATTCACTATCCAAAATTTCAATGTGATCAAATATTTTTAATTCAAAGTATCGTTTTGAATATTCGTTAATTTCTTTCAAGGACCAAATTGTTTTATGCTTATAGTTTTGGTAACAAAAAGTAAAATAAAGTCAAACAGTTTTCATACGATTTGTTTCAAAGTATCATTTTATATTCACATGTTAGTTATTACAATGATTGTGTTCTTGCCTTATCTTTGTAATTAACTTTCCTTTTTGGTGCTGAATTCTAGTATTAATCTGTGTATGTCTATTGCAGGAAACATCTGCATTCTCATTATCTTCTGGTGTGGGAGCAACAAATGCACTTGTGTTTGAGATTGTGATGACTTTTGGTTTGGTTTACACAGTGTATGCTACTGCAGTAGACCCAAAAAATGGTAGCCTAGGAACAATTGCTCCAATTGCAATTGGTTTCATAGTTGGAGCCAACATCTTAGCAGGTGGTGCATTTGATGGTGCATCCATGAACCCAGCAGTTTCTTTTGGGCCTGCTGTTGTTAGCTGGACATGGGCTAATCATTGGGTCTATTGGGCCGGCCCATTGATCGGTTCTGCTATTGCTGCAGTTGTCTATGAGACTTTCTTTATCACCCCTAGCTCTTATGAACAGTTACCGGTCACGGATTATTAA
Protein sequence

>MS.gene67362.t1

MPISRIAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVAASLSHAFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLKIATGGLETSAFSLSSGVGATNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPAVVSWTWANHWVYWAGPLIGSAIAAVVYETFFITPSSYEQLPVTDY