AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61127

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61127.t1 MTR_4g063090 99.206 252 2 0 1 252 1 252 2.46e-177 488
MS.gene61127.t1 MTR_7g023350 77.381 252 57 0 1 252 1 252 1.28e-130 369
MS.gene61127.t1 MTR_1g115405 73.413 252 67 0 1 252 1 252 3.73e-129 365
MS.gene61127.t1 MTR_7g103030 75.000 252 61 2 1 252 1 250 8.41e-121 344
MS.gene61127.t1 MTR_2g101370 64.876 242 84 1 6 247 5 245 1.74e-100 293
MS.gene61127.t1 MTR_8g013680 61.475 244 85 2 14 251 6 246 1.95e-97 285
MS.gene61127.t1 MTR_1g077540 52.326 258 117 1 1 252 1 258 7.60e-94 276
MS.gene61127.t1 MTR_6g069040 54.510 255 113 1 1 252 1 255 1.99e-89 265
MS.gene61127.t1 MTR_5g012810 59.274 248 98 2 5 252 3 247 5.19e-85 253
MS.gene61127.t1 MTR_1g006490 53.361 238 107 2 3 240 1 234 2.05e-84 252
MS.gene61127.t1 MTR_1g006590 50.612 245 117 2 3 247 1 241 8.26e-81 243
MS.gene61127.t1 MTR_2g060360 43.578 218 122 1 18 235 16 232 4.46e-57 182
MS.gene61127.t1 MTR_2g094270 36.066 244 131 6 12 235 31 269 2.81e-38 135
MS.gene61127.t1 MTR_7g101190 36.929 241 136 6 10 235 28 267 1.40e-36 131
MS.gene61127.t1 MTR_3g118010 36.555 238 136 6 21 247 37 270 2.86e-34 124
MS.gene61127.t1 MTR_4g059390 33.745 243 137 6 12 235 30 267 2.47e-31 117
MS.gene61127.t1 MTR_8g098375 33.193 238 144 5 10 235 41 275 3.57e-31 117
MS.gene61127.t1 MTR_5g063930 35.146 239 133 5 18 245 34 261 1.53e-30 114
MS.gene61127.t1 MTR_1g095070 40.722 194 94 6 69 247 25 212 6.29e-30 112
MS.gene61127.t1 MTR_4g415300 34.483 232 126 7 21 235 56 278 1.59e-29 112
MS.gene61127.t1 MTR_3g070210 33.195 241 138 6 10 234 44 277 2.98e-29 112
MS.gene61127.t1 MTR_5g010150 33.613 238 143 6 10 235 41 275 5.97e-29 110
MS.gene61127.t1 MTR_2g094270 35.814 215 116 5 12 209 31 240 3.20e-28 108
MS.gene61127.t1 MTR_5g082070 32.911 237 144 5 10 234 44 277 3.48e-28 108
MS.gene61127.t1 MTR_4g084880 32.751 229 147 4 20 247 34 256 1.28e-25 102
MS.gene61127.t1 MTR_2g017620 33.624 229 143 5 20 247 23 243 1.66e-25 101
MS.gene61127.t1 MTR_2g017620 33.624 229 143 5 20 247 37 257 2.32e-25 101
MS.gene61127.t1 MTR_2g017590 33.188 229 144 5 20 247 35 255 7.36e-24 97.1
MS.gene61127.t1 MTR_2g017620 36.842 171 104 3 78 247 9 176 2.03e-23 94.0
MS.gene61127.t1 MTR_3g008920 35.616 219 127 6 20 235 25 232 2.21e-23 95.9
MS.gene61127.t1 MTR_5g079570 32.727 220 132 6 20 235 39 246 2.31e-22 93.2
MS.gene61127.t1 MTR_2g104940 33.498 203 127 4 25 227 84 278 2.83e-22 93.6
MS.gene61127.t1 MTR_8g087710 32.314 229 146 5 20 247 38 258 1.18e-21 91.3
MS.gene61127.t1 MTR_7g010650 30.705 241 141 6 21 250 37 262 3.19e-21 90.1
MS.gene61127.t1 MTR_2g017660 29.365 252 167 5 1 248 16 260 8.72e-21 89.0
MS.gene61127.t1 MTR_1g095070 38.650 163 80 5 69 216 25 182 1.19e-20 86.7
MS.gene61127.t1 MTR_8g087720 32.129 249 151 7 8 251 21 256 1.80e-20 88.2
MS.gene61127.t1 MTR_3g070210 31.416 226 132 6 10 219 44 262 3.11e-20 87.4
MS.gene61127.t1 MTR_4g006730 31.401 207 134 3 21 227 75 273 1.09e-19 86.7
MS.gene61127.t1 MTR_1g097840 32.719 217 124 8 25 234 78 279 5.27e-19 84.7
MS.gene61127.t1 MTR_2g017610 31.330 233 146 7 20 251 37 256 1.19e-18 83.2
MS.gene61127.t1 MTR_2g017570 31.330 233 146 7 20 251 37 256 1.79e-18 82.8
MS.gene61127.t1 MTR_4g059390 32.778 180 104 5 12 179 30 204 6.39e-18 79.7
MS.gene61127.t1 MTR_4g006630 30.412 194 127 3 34 227 1 186 4.69e-17 77.8
MS.gene61127.t1 MTR_4g006730 30.412 194 127 3 34 227 1 186 4.73e-17 77.8
MS.gene61127.t1 MTR_1g070385 29.630 216 142 6 21 235 62 268 1.19e-16 79.3
MS.gene61127.t1 MTR_8g098375 30.337 178 116 3 10 182 41 215 1.30e-16 77.0
MS.gene61127.t1 MTR_4g415300 33.136 169 94 5 21 179 56 215 1.87e-16 76.3
MS.gene61127.t1 MTR_3g070210 30.899 178 107 4 10 178 44 214 1.10e-15 73.9
MS.gene61127.t1 MTR_5g010150 32.000 175 111 4 10 179 41 212 2.10e-15 73.2
MS.gene61127.t1 MTR_4g075570 28.962 183 104 6 69 229 96 274 6.19e-13 67.8
MS.gene61127.t1 MTR_4g075590 26.582 237 149 6 13 229 29 260 2.22e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61127.t1 AT2G36830 80.159 252 49 1 1 252 1 251 2.07e-142 399
MS.gene61127.t1 AT4G01470 77.381 252 57 0 1 252 1 252 8.96e-139 390
MS.gene61127.t1 AT3G26520 75.889 253 60 1 1 252 1 253 3.62e-133 376
MS.gene61127.t1 AT5G47450 60.324 247 96 1 5 251 3 247 1.05e-95 281
MS.gene61127.t1 AT3G16240 58.000 250 105 0 3 252 1 250 1.64e-94 278
MS.gene61127.t1 AT1G73190 57.917 240 95 2 9 243 12 250 1.02e-89 266
MS.gene61127.t1 AT1G17810 57.851 242 96 2 9 245 12 252 3.13e-88 263
MS.gene61127.t1 AT4G17340 59.514 247 98 1 5 251 3 247 6.01e-86 256
MS.gene61127.t1 AT2G25810 53.012 249 110 3 3 251 1 242 6.15e-80 241
MS.gene61127.t1 AT3G47440 42.922 219 124 1 18 236 20 237 9.11e-53 172
MS.gene61127.t1 AT2G37170 36.822 258 140 8 11 247 21 276 6.53e-36 129
MS.gene61127.t1 AT2G37170 36.822 258 140 8 11 247 64 319 8.03e-36 130
MS.gene61127.t1 AT3G53420 37.450 251 140 7 12 247 30 278 1.14e-35 128
MS.gene61127.t1 AT3G53420 37.450 251 140 7 12 247 30 278 1.14e-35 128
MS.gene61127.t1 AT3G54820 34.855 241 141 5 10 235 27 266 1.06e-34 126
MS.gene61127.t1 AT2G37180 35.938 256 141 8 10 247 26 276 4.11e-34 124
MS.gene61127.t1 AT2G39010 35.102 245 131 7 12 235 29 266 5.09e-34 124
MS.gene61127.t1 AT4G35100 36.134 238 137 6 21 247 38 271 1.14e-33 123
MS.gene61127.t1 AT4G35100 36.134 238 137 6 21 247 38 271 1.14e-33 123
MS.gene61127.t1 AT5G60660 35.060 251 146 7 12 247 30 278 1.58e-33 123
MS.gene61127.t1 AT2G16850 35.294 238 139 6 21 247 36 269 4.17e-32 119
MS.gene61127.t1 AT4G00430 33.745 243 135 7 10 235 42 275 9.29e-30 113
MS.gene61127.t1 AT4G23400 34.052 232 127 6 21 235 53 275 8.29e-29 110
MS.gene61127.t1 AT3G61430 35.065 231 124 7 21 234 52 273 5.12e-28 108
MS.gene61127.t1 AT3G61430 35.065 231 124 7 21 234 52 273 5.12e-28 108
MS.gene61127.t1 AT1G01620 34.052 232 127 7 21 235 52 274 5.12e-28 108
MS.gene61127.t1 AT2G45960 33.884 242 134 7 10 234 41 273 1.06e-27 107
MS.gene61127.t1 AT1G01620 37.500 184 91 6 69 235 26 202 1.37e-25 100
MS.gene61127.t1 AT1G52180 56.627 83 36 0 134 216 42 124 9.04e-24 93.2
MS.gene61127.t1 AT1G80760 32.579 221 140 5 25 245 84 295 9.15e-23 95.1
MS.gene61127.t1 AT4G19030 29.804 255 157 6 8 247 34 281 2.44e-21 90.9
MS.gene61127.t1 AT5G37810 30.837 227 150 3 21 247 43 262 4.23e-21 90.1
MS.gene61127.t1 AT5G37820 29.961 257 161 5 3 247 13 262 1.35e-20 88.6
MS.gene61127.t1 AT5G37820 29.961 257 161 5 3 247 49 298 2.19e-20 88.6
MS.gene61127.t1 AT2G39010 33.149 181 100 5 12 178 29 202 9.18e-20 85.1
MS.gene61127.t1 AT4G18910 28.814 236 152 6 20 247 51 278 6.51e-19 84.3
MS.gene61127.t1 AT2G45960 32.143 224 126 7 10 216 41 255 1.12e-18 83.2
MS.gene61127.t1 AT2G45960 32.143 224 126 7 10 216 41 255 1.28e-18 83.6
MS.gene61127.t1 AT3G06100 27.273 220 153 4 18 237 43 255 2.15e-18 82.4
MS.gene61127.t1 AT1G31885 29.237 236 151 4 20 247 42 269 2.92e-18 82.8
MS.gene61127.t1 AT4G10380 31.163 215 130 5 25 234 82 283 4.44e-18 82.0
MS.gene61127.t1 AT1G31885 33.146 178 108 3 78 247 41 215 1.81e-17 79.7
MS.gene61127.t1 AT3G06100 27.700 213 147 4 24 236 1 206 3.90e-17 78.2
MS.gene61127.t1 AT4G00430 32.022 178 102 5 10 177 42 210 3.12e-15 72.8
MS.gene61127.t1 AT3G06100 31.061 132 89 2 105 236 44 173 2.15e-13 67.4

Find 58 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGTTGGTGCTAACATTTC+TGG 0.108507 4.4:-42765568 MS.gene61127:CDS
GGGCTAATCATTGGGTCTAT+TGG 0.263332 4.4:-42764647 MS.gene61127:CDS
ATGAACCCAGCAGTTTCTTT+TGG 0.263396 4.4:-42764693 MS.gene61127:CDS
GTTAACCCTGCTGTCACTTT+TGG 0.284147 4.4:-42765538 MS.gene61127:CDS
ATTGGAAATCCTTCTGAGTT+TGG 0.293211 4.4:-42765920 MS.gene61127:CDS
TTTGAGATTGTGATGACTTT+TGG 0.309198 4.4:-42764846 MS.gene61127:CDS
TTACCAGTCACGGATTATTA+AGG 0.336094 4.4:-42764546 None:intergenic
ATTGCAATTGGTTTCATAGT+TGG 0.339661 4.4:-42764756 MS.gene61127:CDS
CTGCTGTTGTTAGCTGGACA+TGG 0.355517 4.4:-42764668 MS.gene61127:CDS
AAAAGAAACTGCTGGGTTCA+TGG 0.357172 4.4:+42764695 None:intergenic
CCAATTGCAATTCTAGAAAT+CGG 0.358019 4.4:+42765938 None:intergenic
CCGATTTCTAGAATTGCAAT+TGG 0.370439 4.4:-42765938 MS.gene61127:CDS
TTGGGCCTGCTGTTGTTAGC+TGG 0.373980 4.4:-42764674 MS.gene61127:CDS
GGCTAATCATTGGGTCTATT+GGG 0.374997 4.4:-42764646 MS.gene61127:CDS
GTCTTTGCAGGGGAAGGCTC+TGG 0.375970 4.4:-42765842 MS.gene61127:CDS
TGGCAAAGCTGATGCACTTA+AGG 0.381290 4.4:-42765900 MS.gene61127:CDS
GCTGGACATGGGCTAATCAT+TGG 0.392902 4.4:-42764656 MS.gene61127:CDS
TCTGCATTCTCATTATCTTC+TGG 0.394809 4.4:-42764894 MS.gene61127:CDS
TATTGGATTGCTCAGTTGCT+TGG 0.424094 4.4:-42765472 MS.gene61127:CDS
TTAGCAGGTGGTGCATTTGA+TGG 0.429164 4.4:-42764723 MS.gene61127:CDS
ACAATTGCTCCAATTGCAAT+TGG 0.430105 4.4:-42764768 MS.gene61127:CDS
CAATTGGAGCAATTGTTCCT+AGG 0.454950 4.4:+42764775 None:intergenic
TTCTCATTATCTTCTGGTGT+GGG 0.456268 4.4:-42764888 MS.gene61127:CDS
GGACACATTACACTCATTAG+AGG 0.456484 4.4:-42765502 MS.gene61127:CDS
ACTATGAAACCAATTGCAAT+TGG 0.458046 4.4:+42764759 None:intergenic
TATTGGGCCGGCCCATTGAT+CGG 0.462419 4.4:-42764630 MS.gene61127:CDS
GCAATAGCAGAACCGATCAA+TGG 0.473518 4.4:+42764618 None:intergenic
CTGGACATGGGCTAATCATT+GGG 0.474402 4.4:-42764655 MS.gene61127:CDS
CAATAGCAGAACCGATCAAT+GGG 0.488984 4.4:+42764619 None:intergenic
AATGGTGCAGCAACACCAGC+AGG 0.497841 4.4:-42765640 MS.gene61127:CDS
GCAGGCCCAAAAGAAACTGC+TGG 0.500317 4.4:+42764687 None:intergenic
GGTAACTGTTCATAAGAGCT+AGG 0.503088 4.4:+42764564 None:intergenic
TAAAATTGCCACTGGTGGAC+TGG 0.504856 4.4:-42765425 MS.gene61127:intron
CTCCTTAAAATTGCCACTGG+TGG 0.510876 4.4:-42765430 MS.gene61127:CDS
TGCAGGGGAAGGCTCTGGCA+TGG 0.516920 4.4:-42765837 MS.gene61127:CDS
TTGCTCCTTAAAATTGCCAC+TGG 0.517240 4.4:-42765433 MS.gene61127:CDS
AATCATTGGGTCTATTGGGC+CGG 0.520340 4.4:-42764642 MS.gene61127:CDS
GTAACTGTTCATAAGAGCTA+GGG 0.528275 4.4:+42764565 None:intergenic
CATGTCCAGCTAACAACAGC+AGG 0.530958 4.4:+42764669 None:intergenic
AAAGCACCAAAAGTGACAGC+AGG 0.532536 4.4:+42765532 None:intergenic
AGGCCTTAATAATCCGTGAC+TGG 0.533115 4.4:+42764543 None:intergenic
GTTGGAGCCAACATCTTAGC+AGG 0.533340 4.4:-42764738 MS.gene61127:CDS
TGCTGTTGTTAGCTGGACAT+GGG 0.535749 4.4:-42764667 MS.gene61127:CDS
ATTCTCATTATCTTCTGGTG+TGG 0.543635 4.4:-42764889 MS.gene61127:CDS
TGCACCACCTGCTAAGATGT+TGG 0.559448 4.4:+42764731 None:intergenic
AGCAGAACCGATCAATGGGC+CGG 0.562596 4.4:+42764623 None:intergenic
TCAGCTTTGCCAAACTCAGA+AGG 0.564993 4.4:+42765911 None:intergenic
GTTGGTGCTAACATTTCTGG+TGG 0.567619 4.4:-42765565 MS.gene61127:CDS
ACAGATAAGCTTACAAACAA+TGG 0.573110 4.4:-42765658 MS.gene61127:intron
TGCAGCAACACCAGCAGGGT+TGG 0.574909 4.4:-42765635 MS.gene61127:CDS
GATGCAGCTACCAACCCTGC+TGG 0.592819 4.4:+42765625 None:intergenic
TTATGAACAGTTACCAGTCA+CGG 0.593635 4.4:-42764556 MS.gene61127:CDS
CAGGCCCAAAAGAAACTGCT+GGG 0.603420 4.4:+42764688 None:intergenic
GTTTCTCACCAGTCCACCAG+TGG 0.659307 4.4:+42765417 None:intergenic
AAGCACCAAAAGTGACAGCA+GGG 0.671108 4.4:+42765533 None:intergenic
TAACTGTTCATAAGAGCTAG+GGG 0.673567 4.4:+42764566 None:intergenic
ATGGTGCAGCAACACCAGCA+GGG 0.686064 4.4:-42765639 MS.gene61127:CDS
GGAGCCAACATCTTAGCAGG+TGG 0.690619 4.4:-42764735 MS.gene61127:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCAAAATTATAAAACAATT+TGG + chr4.4:42765440-42765459 None:intergenic 15.0%
!! TACAAAAAAGAAAAAAAAGA+AGG + chr4.4:42765512-42765531 None:intergenic 15.0%
!!! ACCAAATTGTTTTATAATTT+TGG - chr4.4:42765436-42765455 MS.gene61127:CDS 15.0%
!!! TAAGTTAAATTGCAAATTTT+CGG + chr4.4:42764716-42764735 None:intergenic 15.0%
!!! TGCTGAATTTTATTATTAAT+TGG - chr4.4:42765550-42765569 MS.gene61127:CDS 15.0%
!! AATAAAATTCAGCACTAAAA+AGG + chr4.4:42765544-42765563 None:intergenic 20.0%
!! ATAAAATTCAGCACTAAAAA+GGG + chr4.4:42765543-42765562 None:intergenic 20.0%
!!! TTTGATTACATTAGAGTTTT+TGG + chr4.4:42765399-42765418 None:intergenic 20.0%
! ACGAATCTAAGAATTCAAAT+CGG + chr4.4:42765195-42765214 None:intergenic 25.0%
!!! TAATTAAATGTTTTGACTCG+AGG - chr4.4:42765249-42765268 MS.gene61127:intron 25.0%
ACAGATAAGCTTACAAACAA+TGG - chr4.4:42764831-42764850 MS.gene61127:CDS 30.0%
ACTATGAAACCAATTGCAAT+TGG + chr4.4:42765733-42765752 None:intergenic 30.0%
ATTCTTAGATTCGTCCATTT+AGG - chr4.4:42765201-42765220 MS.gene61127:intron 30.0%
TTACTTTGTGAACTCTTCTT+GGG - chr4.4:42764795-42764814 MS.gene61127:CDS 30.0%
! ATTGCAATTGGTTTCATAGT+TGG - chr4.4:42765733-42765752 MS.gene61127:intron 30.0%
! CCAATTGCAATTCTAGAAAT+CGG + chr4.4:42764554-42764573 None:intergenic 30.0%
! TCATTAGAGGTCTTTTGTAT+TGG - chr4.4:42765000-42765019 MS.gene61127:intron 30.0%
!! TTTGAGATTGTGATGACTTT+TGG - chr4.4:42765643-42765662 MS.gene61127:CDS 30.0%
AATTGGTGTATGTCTATTGC+AGG - chr4.4:42765567-42765586 MS.gene61127:CDS 35.0%
ACAATTGCTCCAATTGCAAT+TGG - chr4.4:42765721-42765740 MS.gene61127:intron 35.0%
AGACTAACAATTGAGAGAGT+TGG - chr4.4:42765141-42765160 MS.gene61127:intron 35.0%
ATTCTCATTATCTTCTGGTG+TGG - chr4.4:42765600-42765619 MS.gene61127:CDS 35.0%
ATTGGAAATCCTTCTGAGTT+TGG - chr4.4:42764569-42764588 MS.gene61127:CDS 35.0%
CTTACTTTGTGAACTCTTCT+TGG - chr4.4:42764794-42764813 MS.gene61127:CDS 35.0%
GTAACTGTTCATAAGAGCTA+GGG + chr4.4:42765927-42765946 None:intergenic 35.0%
TAACTGTTCATAAGAGCTAG+GGG + chr4.4:42765926-42765945 None:intergenic 35.0%
TACTTTGTGAACTCTTCTTG+GGG - chr4.4:42764796-42764815 MS.gene61127:CDS 35.0%
TCTGCATTCTCATTATCTTC+TGG - chr4.4:42765595-42765614 MS.gene61127:CDS 35.0%
TTATGAACAGTTACCAGTCA+CGG - chr4.4:42765933-42765952 MS.gene61127:CDS 35.0%
TTCTCATTATCTTCTGGTGT+GGG - chr4.4:42765601-42765620 MS.gene61127:CDS 35.0%
! ATTAAGTTTTCAATGCCCCA+AGG + chr4.4:42765331-42765350 None:intergenic 35.0%
!! AAATCGGTTTCTTTTCCTGA+GGG + chr4.4:42765179-42765198 None:intergenic 35.0%
!! ATGCTCATTTTTGTCTTTGC+AGG - chr4.4:42764635-42764654 MS.gene61127:CDS 35.0%
!! CCGATTTCTAGAATTGCAAT+TGG - chr4.4:42764551-42764570 MS.gene61127:CDS 35.0%
!! GATTGTGATGACTTTTGGTT+TGG - chr4.4:42765648-42765667 MS.gene61127:CDS 35.0%
!! TGCTCATTTTTGTCTTTGCA+GGG - chr4.4:42764636-42764655 MS.gene61127:CDS 35.0%
!!! CTTTTTGTTGCTGTTTCTGT+TGG - chr4.4:42764906-42764925 MS.gene61127:intron 35.0%
!!! TTCCTAGGCTACCATTTTTT+GGG + chr4.4:42765702-42765721 None:intergenic 35.0%
AAAAGAAACTGCTGGGTTCA+TGG + chr4.4:42765797-42765816 None:intergenic 40.0%
ACTGCAGTAGACCCAAAAAA+TGG - chr4.4:42765688-42765707 MS.gene61127:intron 40.0%
CAATAGCAGAACCGATCAAT+GGG + chr4.4:42765873-42765892 None:intergenic 40.0%
CAATTGGAGCAATTGTTCCT+AGG + chr4.4:42765717-42765736 None:intergenic 40.0%
CATCCTCACACTTTAGGTAT+AGG + chr4.4:42765308-42765327 None:intergenic 40.0%
CTTAGATTCGTCCATTTAGG+AGG - chr4.4:42765204-42765223 MS.gene61127:intron 40.0%
GGACACATTACACTCATTAG+AGG - chr4.4:42764987-42765006 MS.gene61127:intron 40.0%
GGCTAATCATTGGGTCTATT+GGG - chr4.4:42765843-42765862 MS.gene61127:CDS 40.0%
GGTAACTGTTCATAAGAGCT+AGG + chr4.4:42765928-42765947 None:intergenic 40.0%
TATTGGATTGCTCAGTTGCT+TGG - chr4.4:42765017-42765036 MS.gene61127:intron 40.0%
TTGCTCCTTAAAATTGCCAC+TGG - chr4.4:42765056-42765075 MS.gene61127:intron 40.0%
! ATGAACCCAGCAGTTTCTTT+TGG - chr4.4:42765796-42765815 MS.gene61127:intron 40.0%
! ATTTTTGTCTTTGCAGGGGA+AGG - chr4.4:42764641-42764660 MS.gene61127:CDS 40.0%
! TGAACCCAGCAGTTTCTTTT+GGG - chr4.4:42765797-42765816 MS.gene61127:intron 40.0%
!! CAAATCGGTTTCTTTTCCTG+AGG + chr4.4:42765180-42765199 None:intergenic 40.0%
!! GCTCATTTTTGTCTTTGCAG+GGG - chr4.4:42764637-42764656 MS.gene61127:CDS 40.0%
!! TCTGTTGGTGCTAACATTTC+TGG - chr4.4:42764921-42764940 MS.gene61127:intron 40.0%
!!! ACTTTTGGTGCTTTCATTGG+TGG - chr4.4:42764966-42764985 MS.gene61127:intron 40.0%
!!! GTCACTTTTGGTGCTTTCAT+TGG - chr4.4:42764963-42764982 MS.gene61127:intron 40.0%
!!! GTTCCTAGGCTACCATTTTT+TGG + chr4.4:42765703-42765722 None:intergenic 40.0%
AAGCACCAAAAGTGACAGCA+GGG + chr4.4:42764959-42764978 None:intergenic 45.0%
AATCATTGGGTCTATTGGGC+CGG - chr4.4:42765847-42765866 MS.gene61127:CDS 45.0%
CACTTTAGGTATAGGCCCTT+TGG + chr4.4:42765300-42765319 None:intergenic 45.0%
CTAAAGTGTGAGGATGCCTT+GGG - chr4.4:42765312-42765331 MS.gene61127:intron 45.0%
CTCCTTAAAATTGCCACTGG+TGG - chr4.4:42765059-42765078 MS.gene61127:intron 45.0%
CTCTGGCATGGCATATAGTA+AGG - chr4.4:42764664-42764683 MS.gene61127:CDS 45.0%
GACCCAAAAAATGGTAGCCT+AGG - chr4.4:42765697-42765716 MS.gene61127:intron 45.0%
GCAATAGCAGAACCGATCAA+TGG + chr4.4:42765874-42765893 None:intergenic 45.0%
GGGCTAATCATTGGGTCTAT+TGG - chr4.4:42765842-42765861 MS.gene61127:CDS 45.0%
TAAAGTGTGAGGATGCCTTG+GGG - chr4.4:42765313-42765332 MS.gene61127:intron 45.0%
TCAGCTTTGCCAAACTCAGA+AGG + chr4.4:42764581-42764600 None:intergenic 45.0%
TGCTGTTGTTAGCTGGACAT+GGG - chr4.4:42765822-42765841 MS.gene61127:intron 45.0%
TGGCAAAGCTGATGCACTTA+AGG - chr4.4:42764589-42764608 MS.gene61127:CDS 45.0%
TTAGCAGGTGGTGCATTTGA+TGG - chr4.4:42765766-42765785 MS.gene61127:intron 45.0%
TTAGTCTCATAGGCCATAGC+AGG - chr4.4:42765273-42765292 MS.gene61127:intron 45.0%
! AAAGCACCAAAAGTGACAGC+AGG + chr4.4:42764960-42764979 None:intergenic 45.0%
! CTGGACATGGGCTAATCATT+GGG - chr4.4:42765834-42765853 MS.gene61127:CDS 45.0%
! GTCCACCAGTGGCAATTTTA+AGG + chr4.4:42765064-42765083 None:intergenic 45.0%
! TAAAATTGCCACTGGTGGAC+TGG - chr4.4:42765064-42765083 MS.gene61127:intron 45.0%
!! GACTCGAGGATTAGTCTCAT+AGG - chr4.4:42765263-42765282 MS.gene61127:intron 45.0%
!! GTTGGTGCTAACATTTCTGG+TGG - chr4.4:42764924-42764943 MS.gene61127:intron 45.0%
!!! GTTAACCCTGCTGTCACTTT+TGG - chr4.4:42764951-42764970 MS.gene61127:intron 45.0%
ATAGGCCATAGCAGGTCCAA+AGG - chr4.4:42765281-42765300 MS.gene61127:intron 50.0%
CAGGCCCAAAAGAAACTGCT+GGG + chr4.4:42765804-42765823 None:intergenic 50.0%
CATGTCCAGCTAACAACAGC+AGG + chr4.4:42765823-42765842 None:intergenic 50.0%
CCTAAAGTGTGAGGATGCCT+TGG - chr4.4:42765311-42765330 MS.gene61127:intron 50.0%
CTGCTGTTGTTAGCTGGACA+TGG - chr4.4:42765821-42765840 MS.gene61127:intron 50.0%
GAGTCGAACTGCCTCCTAAA+TGG + chr4.4:42765218-42765237 None:intergenic 50.0%
GGGCCTATACCTAAAGTGTG+AGG - chr4.4:42765302-42765321 MS.gene61127:intron 50.0%
GTTGGAGCCAACATCTTAGC+AGG - chr4.4:42765751-42765770 MS.gene61127:intron 50.0%
TAGGCCATAGCAGGTCCAAA+GGG - chr4.4:42765282-42765301 MS.gene61127:intron 50.0%
TGCACCACCTGCTAAGATGT+TGG + chr4.4:42765761-42765780 None:intergenic 50.0%
! CCAAGGCATCCTCACACTTT+AGG + chr4.4:42765314-42765333 None:intergenic 50.0%
! GCTGGACATGGGCTAATCAT+TGG - chr4.4:42765833-42765852 MS.gene61127:CDS 50.0%
AATGGTGCAGCAACACCAGC+AGG - chr4.4:42764849-42764868 MS.gene61127:CDS 55.0%
AGCAGAACCGATCAATGGGC+CGG + chr4.4:42765869-42765888 None:intergenic 55.0%
ATGGTGCAGCAACACCAGCA+GGG - chr4.4:42764850-42764869 MS.gene61127:CDS 55.0%
GCAGGCCCAAAAGAAACTGC+TGG + chr4.4:42765805-42765824 None:intergenic 55.0%
GGAGCCAACATCTTAGCAGG+TGG - chr4.4:42765754-42765773 MS.gene61127:intron 55.0%
GTTTCTCACCAGTCCACCAG+TGG + chr4.4:42765075-42765094 None:intergenic 55.0%
TAGGCCCTTTGGACCTGCTA+TGG + chr4.4:42765289-42765308 None:intergenic 55.0%
TATTGGGCCGGCCCATTGAT+CGG - chr4.4:42765859-42765878 MS.gene61127:CDS 55.0%
TGGTAACACGACTCTCCCTC+AGG - chr4.4:42765161-42765180 MS.gene61127:intron 55.0%
TTGGGCCTGCTGTTGTTAGC+TGG - chr4.4:42765815-42765834 MS.gene61127:intron 55.0%
GATGCAGCTACCAACCCTGC+TGG + chr4.4:42764867-42764886 None:intergenic 60.0%
GTCTTTGCAGGGGAAGGCTC+TGG - chr4.4:42764647-42764666 MS.gene61127:CDS 60.0%
TGCAGCAACACCAGCAGGGT+TGG - chr4.4:42764854-42764873 MS.gene61127:CDS 60.0%
! TGCAGGGGAAGGCTCTGGCA+TGG - chr4.4:42764652-42764671 MS.gene61127:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 42764548 42765963 42764548 ID=MS.gene61127
chr4.4 mRNA 42764548 42765963 42764548 ID=MS.gene61127.t1;Parent=MS.gene61127
chr4.4 exon 42765831 42765963 42765831 ID=MS.gene61127.t1.exon1;Parent=MS.gene61127.t1
chr4.4 CDS 42765831 42765963 42765831 ID=cds.MS.gene61127.t1;Parent=MS.gene61127.t1
chr4.4 exon 42765426 42765676 42765426 ID=MS.gene61127.t1.exon2;Parent=MS.gene61127.t1
chr4.4 CDS 42765426 42765676 42765426 ID=cds.MS.gene61127.t1;Parent=MS.gene61127.t1
chr4.4 exon 42764548 42764922 42764548 ID=MS.gene61127.t1.exon3;Parent=MS.gene61127.t1
chr4.4 CDS 42764548 42764922 42764548 ID=cds.MS.gene61127.t1;Parent=MS.gene61127.t1
Gene Sequence

>MS.gene61127

ATGCCGATTTCTAGAATTGCAATTGGAAATCCTTCTGAGTTTGGCAAAGCTGATGCACTTAAGGCTGCACTAGCTGAGTTCATTTCAATGCTCATTTTTGTCTTTGCAGGGGAAGGCTCTGGCATGGCATATAGTAAGGCTTTTTATTAGTTAATTAAACTCTTTCCGAAAATTTGCAATTTAACTTATTTGAGTTTATCTACTTACATAAACATTTGTGTAACTATTTCAAAAAACTGTTTTCAACTTACTTTGTGAACTCTTCTTGGGGTGTGTTTTTTTGACAGATAAGCTTACAAACAATGGTGCAGCAACACCAGCAGGGTTGGTAGCTGCATCACTATCACATGCATTTGCACTTTTTGTTGCTGTTTCTGTTGGTGCTAACATTTCTGGTGGACATGTTAACCCTGCTGTCACTTTTGGTGCTTTCATTGGTGGACACATTACACTCATTAGAGGTCTTTTGTATTGGATTGCTCAGTTGCTTGGTTCTGTTGTTGCTTGCTTGCTCCTTAAAATTGCCACTGGTGGACTGGTGAGAAACATTACATATATACTATTCATTCATTCATTCATTCTAAATTCAATCAAGACTAACAATTGAGAGAGTTGGTAACACGACTCTCCCTCAGGAAAAGAAACCGATTTGAATTCTTAGATTCGTCCATTTAGGAGGCAGTTCGACTCTATGTCAAAACTAATTAAATGTTTTGACTCGAGGATTAGTCTCATAGGCCATAGCAGGTCCAAAGGGCCTATACCTAAAGTGTGAGGATGCCTTGGGGCATTGAAAACTTAATAATTTCAATCATGTTAGTTTTAATTTTATAGACTAAATTGACTATCCAAAAACTCTAATGTAATCAAAGATTTTTAGTTTAAGAGACCAAATTGTTTTATAATTTTGGTGACTAAATTTTAGATTCACTTGTTAGTTATTACAATCACTCTATTCTTGCCTTCTTTTTTTTCTTTTTTGTAATTAACTTCCCTTTTTAGTGCTGAATTTTATTATTAATTGGTGTATGTCTATTGCAGGAAACATCTGCATTCTCATTATCTTCTGGTGTGGGAGCAACAAAAGCACTTGTGTTTGAGATTGTGATGACTTTTGGTTTGGTTTACACAGTGTATGCTACTGCAGTAGACCCAAAAAATGGTAGCCTAGGAACAATTGCTCCAATTGCAATTGGTTTCATAGTTGGAGCCAACATCTTAGCAGGTGGTGCATTTGATGGTGCATCCATGAACCCAGCAGTTTCTTTTGGGCCTGCTGTTGTTAGCTGGACATGGGCTAATCATTGGGTCTATTGGGCCGGCCCATTGATCGGTTCTGCTATTGCTGCAGTTGTCTATGAGACTTTCTTTATCACCCCTAGCTCTTATGAACAGTTACCAGTCACGGATTATTAA
Protein sequence

>MS.gene61127.t1

MPISRIAIGNPSEFGKADALKAALAEFISMLIFVFAGEGSGMAYNKLTNNGAATPAGLVAASLSHAFALFVAVSVGANISGGHVNPAVTFGAFIGGHITLIRGLLYWIAQLLGSVVACLLLKIATGGLETSAFSLSSGVGATKALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPAVVSWTWANHWVYWAGPLIGSAIAAVVYETFFITPSSYEQLPVTDY