AlfalfaGEDB | MS.gene31709

M. sativa cultivar XinJiangDaYe / MS.gene31709

KEGG: K09873
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0015267 GO:0016020 GO:0022803 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00230.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31709.t1	RDX78845.1	87.4	246	25	1	1	240	1	246	2.20E-114	421.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31709.t1	O82316	66.9	245	75	1	1	239	1	245	1.1e-85	317.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31709.t1	A0A371FKI7	87.4	246	25	1	1	240	1	246	1.6e-114	421.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051749	MS.gene31709	0.833577	4.92E-56	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene31709	MS.gene03094	PPI
MS.gene75156	MS.gene31709	PPI
MS.gene005974	MS.gene31709	PPI
MS.gene31709	MS.gene67362	PPI
MS.gene31709	MS.gene021061	PPI
MS.gene31709	MS.gene03090	PPI
MS.gene31709	MS.gene025449	PPI
MS.gene31709	MS.gene21620	PPI
MS.gene31709	MS.gene33486	PPI
MS.gene31709	MS.gene048817	PPI
MS.gene31709	MS.gene27853	PPI
MS.gene31709	MS.gene069812	PPI
MS.gene31709	MS.gene79774	PPI
MS.gene31709	MS.gene92450	PPI
MS.gene31709	MS.gene01625	PPI
MS.gene31709	MS.gene032497	PPI
MS.gene31709	MS.gene89899	PPI
MS.gene31709	MS.gene065973	PPI
MS.gene31709	MS.gene002643	PPI
MS.gene31709	MS.gene072777	PPI
MS.gene31709	MS.gene004324	PPI
MS.gene31709	MS.gene83316	PPI
MS.gene31709	MS.gene004088	PPI
MS.gene31709	MS.gene065972	PPI
MS.gene31709	MS.gene052743	PPI
MS.gene31709	MS.gene014206	PPI
MS.gene31709	MS.gene004322	PPI
MS.gene31709	MS.gene023621	PPI
MS.gene31709	MS.gene061432	PPI
MS.gene34830	MS.gene31709	PPI
MS.gene31709	MS.gene023622	PPI
MS.gene31709	MS.gene019314	PPI
MS.gene31709	MS.gene042849	PPI
MS.gene31709	MS.gene002639	PPI
MS.gene025056	MS.gene31709	PPI
MS.gene31709	MS.gene037437	PPI
MS.gene31709	MS.gene61127	PPI
MS.gene31709	MS.gene90854	PPI
MS.gene31709	MS.gene01624	PPI
MS.gene31709	MS.gene029129	PPI
MS.gene31709	MS.gene03121	PPI
MS.gene31709	MS.gene93201	PPI
MS.gene31709	MS.gene024637	PPI
MS.gene03093	MS.gene31709	PPI
MS.gene31709	MS.gene01627	PPI
MS.gene31709	MS.gene004953	PPI
MS.gene31709	MS.gene71137	PPI
MS.gene31709	MS.gene062190	PPI
MS.gene31709	MS.gene54792	PPI
MS.gene31709	MS.gene49432	PPI
MS.gene31709	MS.gene004321	PPI
MS.gene31709	MS.gene72127	PPI
MS.gene31709	MS.gene72129	PPI
MS.gene31709	MS.gene002640	PPI
MS.gene31709	MS.gene27852	PPI
MS.gene34032	MS.gene31709	PPI
MS.gene07481	MS.gene31709	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31709.t1	MTR_1g006490	97.166	247	1	1	1	241	1	247	7.32e-171	470
MS.gene31709.t1	MTR_1g006590	85.020	247	31	1	1	241	1	247	1.37e-151	422
MS.gene31709.t1	MTR_8g013680	56.452	248	96	4	1	237	1	247	1.01e-86	258
MS.gene31709.t1	MTR_2g101370	54.918	244	100	3	4	237	5	248	6.11e-85	253
MS.gene31709.t1	MTR_4g063090	52.083	240	105	3	1	230	3	242	1.20e-76	232
MS.gene31709.t1	MTR_7g103030	53.279	244	103	4	1	234	3	245	8.71e-76	230
MS.gene31709.t1	MTR_1g115405	50.000	240	109	4	3	232	5	243	1.38e-74	227
MS.gene31709.t1	MTR_7g023350	51.852	243	106	4	3	234	5	247	1.28e-73	224
MS.gene31709.t1	MTR_5g012810	51.807	249	106	5	1	238	1	246	3.03e-73	223
MS.gene31709.t1	MTR_1g077540	42.688	253	128	3	3	238	5	257	1.23e-65	204
MS.gene31709.t1	MTR_6g069040	43.254	252	125	4	3	238	5	254	7.69e-65	202
MS.gene31709.t1	MTR_2g060360	36.800	250	144	5	1	237	1	249	1.72e-44	150
MS.gene31709.t1	MTR_5g063930	33.473	239	138	7	14	241	32	260	5.58e-30	112
MS.gene31709.t1	MTR_2g017620	35.931	231	137	6	18	241	23	249	1.66e-29	111
MS.gene31709.t1	MTR_2g017620	35.931	231	137	6	18	241	37	263	3.32e-29	110
MS.gene31709.t1	MTR_2g094270	34.008	247	129	10	11	228	32	273	6.29e-29	110
MS.gene31709.t1	MTR_7g101190	33.061	245	137	7	9	228	29	271	6.92e-29	110
MS.gene31709.t1	MTR_3g118010	34.335	233	124	8	19	228	37	263	3.69e-28	108
MS.gene31709.t1	MTR_2g017590	35.065	231	139	6	18	241	35	261	2.85e-27	105
MS.gene31709.t1	MTR_4g415300	35.088	228	120	8	19	223	56	278	6.50e-27	105
MS.gene31709.t1	MTR_5g079570	35.242	227	138	6	18	239	39	261	6.73e-27	104
MS.gene31709.t1	MTR_1g097840	35.498	231	114	7	19	231	74	287	9.00e-27	105
MS.gene31709.t1	MTR_4g006730	36.585	205	104	8	24	214	80	272	9.04e-27	105
MS.gene31709.t1	MTR_5g010150	34.199	231	119	8	19	223	52	275	1.52e-26	104
MS.gene31709.t1	MTR_2g104940	36.585	205	104	8	24	214	85	277	3.15e-26	103
MS.gene31709.t1	MTR_4g059390	33.202	253	124	11	10	228	30	271	4.75e-26	102
MS.gene31709.t1	MTR_8g098375	33.333	231	121	8	19	223	52	275	3.82e-25	100
MS.gene31709.t1	MTR_3g008920	37.037	216	123	7	18	226	25	234	4.20e-25	100
MS.gene31709.t1	MTR_2g017660	33.913	230	134	9	18	238	38	258	4.36e-25	100
MS.gene31709.t1	MTR_4g006730	37.195	164	88	4	57	214	31	185	6.29e-25	98.6
MS.gene31709.t1	MTR_4g006630	37.195	164	88	4	57	214	31	185	6.35e-25	98.6
MS.gene31709.t1	MTR_5g082070	34.091	220	124	6	19	218	55	273	2.37e-24	98.6
MS.gene31709.t1	MTR_1g095070	34.737	190	100	6	56	229	24	205	3.22e-24	96.7
MS.gene31709.t1	MTR_3g070210	33.333	228	131	7	11	218	47	273	4.22e-24	97.8
MS.gene31709.t1	MTR_8g087710	32.864	213	132	6	18	223	38	246	8.79e-23	94.0
MS.gene31709.t1	MTR_7g010650	33.810	210	129	5	19	222	37	242	2.11e-22	93.2
MS.gene31709.t1	MTR_4g084880	34.375	224	116	8	18	223	34	244	2.27e-22	92.8
MS.gene31709.t1	MTR_2g017610	32.883	222	131	7	7	214	19	236	3.30e-22	92.4
MS.gene31709.t1	MTR_2g017620	35.359	181	111	4	63	241	6	182	6.18e-22	89.7
MS.gene31709.t1	MTR_8g087720	33.178	214	130	7	18	223	37	245	7.32e-22	91.7
MS.gene31709.t1	MTR_2g017570	32.432	222	132	7	7	214	19	236	1.61e-21	90.5
MS.gene31709.t1	MTR_1g070385	30.137	219	142	6	19	231	62	275	5.46e-21	92.0
MS.gene31709.t1	MTR_2g094270	32.735	223	117	9	11	204	32	250	5.50e-20	86.3
MS.gene31709.t1	MTR_4g075590	30.000	260	144	9	10	239	28	279	1.50e-16	77.8
MS.gene31709.t1	MTR_4g075570	27.692	260	153	7	10	241	42	294	1.87e-16	77.4
MS.gene31709.t1	MTR_3g070210	31.100	209	123	7	19	207	55	262	5.25e-16	75.9
MS.gene31709.t1	MTR_1g095070	32.727	165	89	5	56	204	24	182	3.38e-15	72.0
MS.gene31709.t1	MTR_4g006730	35.882	170	83	8	24	179	80	237	2.78e-14	70.5
MS.gene31709.t1	MTR_1g097840	34.078	179	84	6	19	179	74	236	1.74e-13	68.6
MS.gene31709.t1	MTR_4g415300	34.615	156	81	6	19	158	56	206	4.79e-13	66.6
MS.gene31709.t1	MTR_5g010150	33.333	159	80	6	19	158	52	203	1.00e-12	65.5
MS.gene31709.t1	MTR_4g059390	32.386	176	82	8	10	158	30	195	2.92e-12	63.9
MS.gene31709.t1	MTR_8g098375	30.994	171	92	6	19	170	52	215	9.54e-12	63.2
MS.gene31709.t1	MTR_3g070210	31.373	153	91	5	19	158	55	206	7.23e-11	60.5
MS.gene31709.t1	MTR_4g006650	33.793	145	65	7	24	155	9	135	9.76e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31709.t1	AT2G25810	77.143	245	50	1	1	239	1	245	2.64e-127	360
MS.gene31709.t1	AT3G16240	54.878	246	98	5	1	234	1	245	6.53e-86	256
MS.gene31709.t1	AT2G36830	54.545	242	100	3	3	234	5	246	3.15e-85	254
MS.gene31709.t1	AT3G26520	54.274	234	97	3	7	230	10	243	6.95e-81	243
MS.gene31709.t1	AT5G47450	52.720	239	103	3	1	229	1	239	5.40e-79	238
MS.gene31709.t1	AT4G01470	50.202	247	112	4	3	238	5	251	3.61e-76	231
MS.gene31709.t1	AT4G17340	53.333	240	100	3	1	229	1	239	7.15e-75	228
MS.gene31709.t1	AT1G73190	47.860	257	110	4	6	238	11	267	2.81e-70	216
MS.gene31709.t1	AT1G17810	48.560	243	110	3	6	233	11	253	5.03e-67	208
MS.gene31709.t1	AT3G47440	39.726	219	121	4	16	224	20	237	5.46e-45	151
MS.gene31709.t1	AT5G60660	34.746	236	126	8	19	229	39	271	2.76e-30	114
MS.gene31709.t1	AT2G37170	33.333	243	126	8	11	223	72	308	8.18e-30	114
MS.gene31709.t1	AT2G37170	33.333	243	126	8	11	223	29	265	9.58e-30	112
MS.gene31709.t1	AT2G37180	34.025	241	129	8	10	223	28	265	1.18e-29	112
MS.gene31709.t1	AT4G35100	35.345	232	123	9	19	228	38	264	4.50e-29	110
MS.gene31709.t1	AT4G35100	35.345	232	123	9	19	228	38	264	4.50e-29	110
MS.gene31709.t1	AT3G54820	33.478	230	127	6	19	223	38	266	7.97e-29	110
MS.gene31709.t1	AT2G39010	32.636	239	135	6	10	223	29	266	2.12e-28	109
MS.gene31709.t1	AT3G53420	33.913	230	126	7	19	223	39	267	2.27e-28	109
MS.gene31709.t1	AT3G53420	33.913	230	126	7	19	223	39	267	2.27e-28	109
MS.gene31709.t1	AT2G16850	34.483	232	125	9	19	228	36	262	9.05e-28	107
MS.gene31709.t1	AT4G00430	35.683	227	120	8	19	223	53	275	5.67e-27	105
MS.gene31709.t1	AT4G19030	36.530	219	123	7	18	223	54	269	7.05e-27	105
MS.gene31709.t1	AT1G80760	35.266	207	114	5	19	214	80	277	1.50e-26	104
MS.gene31709.t1	AT5G37820	37.333	225	119	9	19	231	43	257	5.67e-26	102
MS.gene31709.t1	AT1G01620	34.802	227	122	8	19	223	52	274	6.78e-26	102
MS.gene31709.t1	AT4G23400	34.375	224	127	6	19	223	53	275	9.24e-26	102
MS.gene31709.t1	AT5G37820	36.842	228	121	9	19	234	79	295	9.28e-26	102
MS.gene31709.t1	AT3G61430	36.000	225	112	8	19	218	52	269	2.65e-25	101
MS.gene31709.t1	AT3G61430	36.000	225	112	8	19	218	52	269	2.65e-25	101
MS.gene31709.t1	AT2G45960	36.000	225	112	8	19	218	52	269	3.00e-25	100
MS.gene31709.t1	AT4G10380	33.913	230	126	8	14	228	71	289	6.50e-25	100
MS.gene31709.t1	AT4G18910	35.455	220	124	7	18	223	51	266	7.42e-25	100
MS.gene31709.t1	AT5G37810	35.683	227	122	8	12	223	33	250	7.78e-25	99.8
MS.gene31709.t1	AT1G31885	35.071	211	121	6	18	215	42	249	7.47e-24	97.8
MS.gene31709.t1	AT1G52180	51.163	86	42	0	119	204	39	124	3.31e-23	91.3
MS.gene31709.t1	AT1G01620	34.066	182	102	6	56	223	25	202	1.98e-22	91.7
MS.gene31709.t1	AT1G31885	35.632	174	100	5	50	214	24	194	4.55e-19	84.0
MS.gene31709.t1	AT2G34390	30.594	219	135	7	18	223	47	261	1.07e-17	80.5
MS.gene31709.t1	AT2G45960	33.649	211	108	8	19	204	52	255	4.38e-17	78.6
MS.gene31709.t1	AT3G06100	29.717	212	142	4	18	224	45	254	6.25e-17	78.2
MS.gene31709.t1	AT2G45960	33.649	211	108	8	19	204	52	255	7.08e-17	78.6
MS.gene31709.t1	AT2G34390	30.698	215	134	6	21	223	8	219	9.27e-17	77.4
MS.gene31709.t1	AT3G06100	30.583	206	136	4	24	224	3	206	1.68e-16	76.3
MS.gene31709.t1	AT4G00430	35.484	155	81	6	19	158	53	203	4.09e-13	66.6
MS.gene31709.t1	AT2G39010	31.138	167	96	4	10	158	29	194	4.60e-13	66.6
MS.gene31709.t1	AT4G19030	34.884	172	96	7	18	176	54	222	9.40e-13	65.9
MS.gene31709.t1	AT4G19030	34.000	150	87	5	18	155	54	203	2.07e-11	62.0
MS.gene31709.t1	AT4G18910	34.091	176	98	7	18	179	51	222	3.02e-11	61.6

Find 81 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAGCAAGTGCTCCTTTCTT+TGG	0.189370	1.1:+12619258	None:intergenic
ACCTTTCTCTTTGTCTTTGC+TGG	0.204008	1.1:-12620232	MS.gene31709:CDS
GGACTGATCATTGGGTTTAT+TGG	0.222150	1.1:-12619116	MS.gene31709:CDS
ATGAACCCTGCAAGATCTTT+TGG	0.224227	1.1:-12619162	MS.gene31709:CDS
CTGCTTTGGTTAGTGGAAAT+TGG	0.234876	1.1:-12619137	MS.gene31709:CDS
GGTTGTTCCTAACGCAATTT+TGG	0.235450	1.1:+12620309	None:intergenic
TATTGGGTTGGACCACTTAT+TGG	0.235959	1.1:-12619099	MS.gene31709:CDS
GGCCTGGGCCCAACCTTAGT+TGG	0.240880	1.1:-12619237	MS.gene31709:CDS
CTCTTTGTCTTTGCTGGTGT+TGG	0.290840	1.1:-12620226	MS.gene31709:intron
TTGAATCCCGCCGTGACTTT+GGG	0.304849	1.1:-12619871	MS.gene31709:CDS
TTTGAATCCCGCCGTGACTT+TGG	0.311719	1.1:-12619872	MS.gene31709:CDS
GACTGATCATTGGGTTTATT+GGG	0.313313	1.1:-12619115	MS.gene31709:CDS
CTTATTGGTGGTGGACTTGC+TGG	0.315006	1.1:-12619084	MS.gene31709:CDS
TTTGGGCCTGCTTTGGTTAG+TGG	0.316883	1.1:-12619144	MS.gene31709:CDS
CTTAGTTGGGTTTGTGGTGG+GGG	0.319883	1.1:-12619223	MS.gene31709:CDS
GCCTGGGCCCAACCTTAGTT+GGG	0.322309	1.1:-12619236	MS.gene31709:CDS
ACCTTAGTTGGGTTTGTGGT+GGG	0.325499	1.1:-12619225	MS.gene31709:CDS
ATCTCCGCCGCTCATATTTC+CGG	0.326476	1.1:-12619901	MS.gene31709:CDS
GGGGCTAACATCCTTGCTGG+TGG	0.334049	1.1:-12619204	MS.gene31709:CDS
TCGTTCGTTCGATCCTATAT+TGG	0.337543	1.1:-12619822	MS.gene31709:CDS
CCCAACCTTAGTTGGGTTTG+TGG	0.338677	1.1:-12619229	MS.gene31709:CDS
TATCTTCTTCATTATCTCTC+TGG	0.355133	1.1:-12619766	MS.gene31709:CDS
GAAAGGAGCACTTGCTGGCC+TGG	0.355522	1.1:-12619253	MS.gene31709:CDS
GGGCTAACATCCTTGCTGGT+GGG	0.361838	1.1:-12619203	MS.gene31709:CDS
GGTACACTCAAGGAGTAGTT+TGG	0.375382	1.1:-12619335	MS.gene31709:CDS
GTGGAAATTGGACTGATCAT+TGG	0.389374	1.1:-12619125	MS.gene31709:CDS
TCAATTGGTCAATCCAATAT+AGG	0.391166	1.1:+12619809	None:intergenic
TGGAAATTGGACTGATCATT+GGG	0.400779	1.1:-12619124	MS.gene31709:CDS
TCATTATCTCTCTGGTGGAT+TGG	0.401572	1.1:-12619758	MS.gene31709:intron
CCAAAGAAAGGAGCACTTGC+TGG	0.403446	1.1:-12619258	MS.gene31709:CDS
GTGACTTTGGGCCTCCTTGT+CGG	0.411926	1.1:-12619859	MS.gene31709:CDS
CCTTAGTTGGGTTTGTGGTG+GGG	0.413230	1.1:-12619224	MS.gene31709:CDS
AAAGGAGCACTTGCTGGCCT+GGG	0.419186	1.1:-12619252	MS.gene31709:CDS
GGCTAACATCCTTGCTGGTG+GGG	0.419242	1.1:-12619202	MS.gene31709:CDS
AACCTTAGTTGGGTTTGTGG+TGG	0.423963	1.1:-12619226	MS.gene31709:CDS
ATCAGGCTGAGTAGCCTCTC+TGG	0.431188	1.1:+12620288	None:intergenic
GCTAAGTGGAGATGCCTTAG+TGG	0.435457	1.1:-12619971	MS.gene31709:CDS
GCAGGCCCAAAAGATCTTGC+AGG	0.436765	1.1:+12619156	None:intergenic
GATCATTGGGTTTATTGGGT+TGG	0.446033	1.1:-12619111	MS.gene31709:CDS
GTTGGACCACTTATTGGTGG+TGG	0.458421	1.1:-12619093	MS.gene31709:CDS
CTTCTTCATTATCTCTCTGG+TGG	0.466813	1.1:-12619763	MS.gene31709:CDS
CATACACTTGCAAGTGGAGT+AGG	0.467366	1.1:-12619357	MS.gene31709:CDS
AAGTCACGGCGGGATTCAAA+TGG	0.491922	1.1:+12619875	None:intergenic
GTACACTCAAGGAGTAGTTT+GGG	0.493061	1.1:-12619334	MS.gene31709:CDS
TGGGTTGGACCACTTATTGG+TGG	0.496312	1.1:-12619096	MS.gene31709:CDS
ACCACAAACCCAACTAAGGT+TGG	0.497042	1.1:+12619228	None:intergenic
GGCTATTGCACATGCTCTTG+TGG	0.499860	1.1:-12619935	MS.gene31709:CDS
CAGCTGCAGATGCTATCAAT+TGG	0.500751	1.1:+12619794	None:intergenic
ATTAGAGCTTGAATGCAATC+AGG	0.503619	1.1:+12620271	None:intergenic
CCACAAACCCAACTAAGGTT+GGG	0.515362	1.1:+12619229	None:intergenic
CCCCACCACAAACCCAACTA+AGG	0.515431	1.1:+12619224	None:intergenic
TCCTTTCTTTGGATCAACCA+TGG	0.517654	1.1:+12619269	None:intergenic
GACAAGGAGGCCCAAAGTCA+CGG	0.542123	1.1:+12619861	None:intergenic
ATTTCAATAGATAAGCTAAG+TGG	0.542575	1.1:-12619985	MS.gene31709:intron
ACCATGGTTGATCCAAAGAA+AGG	0.549142	1.1:-12619270	MS.gene31709:CDS
GCAAGTCCACCACCAATAAG+TGG	0.551655	1.1:+12619087	None:intergenic
CAATTTCCACTAACCAAAGC+AGG	0.552637	1.1:+12619138	None:intergenic
AGGAGGCCCAAAGTCACGGC+GGG	0.557990	1.1:+12619865	None:intergenic
ACCAGCAAAGACAAAGAGAA+AGG	0.561490	1.1:+12620231	None:intergenic
ACCATGGCCAAAATTGCGTT+AGG	0.563591	1.1:-12620316	MS.gene31709:CDS
CCACTTGCAAGTGTATGAGC+TGG	0.565802	1.1:+12619363	None:intergenic
CCAGCTCATACACTTGCAAG+TGG	0.572996	1.1:-12619363	MS.gene31709:CDS
CTTTCTTCATCTACAACTAG+AGG	0.584705	1.1:+12619021	None:intergenic
ACCCAACTAAGGTTGGGCCC+AGG	0.593749	1.1:+12619235	None:intergenic
CTAAGTGGAGATGCCTTAGT+GGG	0.595922	1.1:-12619970	MS.gene31709:CDS
GGCGGGATTCAAATGGCCAC+CGG	0.596177	1.1:+12619882	None:intergenic
ACTTTGGGCCTCCTTGTCGG+AGG	0.600598	1.1:-12619856	MS.gene31709:CDS
ATACACTTGCAAGTGGAGTA+GGG	0.603588	1.1:-12619356	MS.gene31709:CDS
TCTTGCAGGGTTCATTGAAG+CGG	0.607315	1.1:+12619170	None:intergenic
GTTCACTGTGTATGCAACCA+TGG	0.609878	1.1:-12619286	MS.gene31709:CDS
CAGGCCCAAAAGATCTTGCA+GGG	0.619708	1.1:+12619157	None:intergenic
TATTGCACATGCTCTTGTGG+TGG	0.628052	1.1:-12619932	MS.gene31709:CDS
TCCGCCGCTCATATTTCCGG+TGG	0.641948	1.1:-12619898	MS.gene31709:CDS
AGCAAAGACAAAGAGAAAGG+TGG	0.654285	1.1:+12620234	None:intergenic
TAGTTATGTGACCTCCGACA+AGG	0.657530	1.1:+12619845	None:intergenic
AGTGGAGTAGGGTACACTCA+AGG	0.668556	1.1:-12619345	MS.gene31709:CDS
GCCACCGGAAATATGAGCGG+CGG	0.678011	1.1:+12619897	None:intergenic
TGCGTTAGGAACAACCAGAG+AGG	0.706593	1.1:-12620302	MS.gene31709:CDS
ATGGCCACCGGAAATATGAG+CGG	0.716960	1.1:+12619894	None:intergenic
TTATGTGACCTCCGACAAGG+AGG	0.729011	1.1:+12619848	None:intergenic
AAGGAGGCCCAAAGTCACGG+CGG	0.732640	1.1:+12619864	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATTTTTCAATTTTATTGTA+TGG	+	chr1.1:12619817-12619836	None:intergenic	10.0%
!!	TGTAATAAATGTAATATTTG+AGG	-	chr1.1:12619698-12619717	MS.gene31709:intron	15.0%
!!!	AAATATTCTTGCTTTTTAAA+GGG	-	chr1.1:12619848-12619867	MS.gene31709:CDS	15.0%
!!!	ATTTTTAAGGTACAAAAAAT+TGG	-	chr1.1:12619661-12619680	MS.gene31709:intron	15.0%
!!!	CAAAACAATTTGATTTTAAA+AGG	-	chr1.1:12619723-12619742	MS.gene31709:intron	15.0%
!!!	CTTTTAAAATCAAATTGTTT+TGG	+	chr1.1:12619725-12619744	None:intergenic	15.0%
!!!	TTTTTAAGGTACAAAAAATT+GGG	-	chr1.1:12619662-12619681	MS.gene31709:intron	15.0%
!!	ACATTTCAATTCAAATAACA+TGG	+	chr1.1:12619246-12619265	None:intergenic	20.0%
!!	ATCTATTGAAATAAATTCCA+AGG	+	chr1.1:12619334-12619353	None:intergenic	20.0%
!!	CAACAATAAAAGACAAAAAA+AGG	+	chr1.1:12619887-12619906	None:intergenic	20.0%
!!	CATTTCAATTCAAATAACAT+GGG	+	chr1.1:12619245-12619264	None:intergenic	20.0%
!!!	AACAATTTGATTTTAAAAGG+AGG	-	chr1.1:12619726-12619745	MS.gene31709:intron	20.0%
!!!	CAAAAATTACCACTTTTTTT+AGG	-	chr1.1:12619626-12619645	MS.gene31709:intron	20.0%
!!!	CAAATATTCTTGCTTTTTAA+AGG	-	chr1.1:12619847-12619866	MS.gene31709:CDS	20.0%
!!!	CTAAAAAAAGTGGTAATTTT+TGG	+	chr1.1:12619628-12619647	None:intergenic	20.0%
!!!	CTTTTTTTGTCTTTTATTGT+TGG	-	chr1.1:12619885-12619904	MS.gene31709:CDS	20.0%
!!!	GTTACAAATCATCATTTTTA+AGG	-	chr1.1:12619648-12619667	MS.gene31709:intron	20.0%
!!!	TCAATTTTATTGTATGGATA+AGG	+	chr1.1:12619811-12619830	None:intergenic	20.0%
!	ATAACATGGGATGAAATTAA+TGG	+	chr1.1:12619232-12619251	None:intergenic	25.0%
!	ATTTCAATAGATAAGCTAAG+TGG	-	chr1.1:12619342-12619361	MS.gene31709:CDS	25.0%
!	GATTTGTAACCTAAAAAAAG+TGG	+	chr1.1:12619638-12619657	None:intergenic	25.0%
!!	TAAAAGGAGGAAACTATTTT+TGG	-	chr1.1:12619739-12619758	MS.gene31709:intron	25.0%
	TATCTTCTTCATTATCTCTC+TGG	-	chr1.1:12619561-12619580	MS.gene31709:intron	30.0%
	TCAATTGGTCAATCCAATAT+AGG	+	chr1.1:12619521-12619540	None:intergenic	30.0%
	ATTAGAGCTTGAATGCAATC+AGG	+	chr1.1:12619059-12619078	None:intergenic	35.0%
	CACAAAAAACAATCCCACTA+AGG	+	chr1.1:12619373-12619392	None:intergenic	35.0%
	CTTTCTTCATCTACAACTAG+AGG	+	chr1.1:12620309-12620328	None:intergenic	35.0%
	GACTGATCATTGGGTTTATT+GGG	-	chr1.1:12620212-12620231	MS.gene31709:intron	35.0%
	TGGAAATTGGACTGATCATT+GGG	-	chr1.1:12620203-12620222	MS.gene31709:intron	35.0%
!	TGGGATGAAATTAATGGATG+AGG	+	chr1.1:12619226-12619245	None:intergenic	35.0%
!!!	CTTAGTGGGATTGTTTTTTG+TGG	-	chr1.1:12619371-12619390	MS.gene31709:CDS	35.0%
	ACCAGCAAAGACAAAGAGAA+AGG	+	chr1.1:12619099-12619118	None:intergenic	40.0%
	ACCATGGTTGATCCAAAGAA+AGG	-	chr1.1:12620057-12620076	MS.gene31709:intron	40.0%
	ACCTTTCTCTTTGTCTTTGC+TGG	-	chr1.1:12619095-12619114	MS.gene31709:CDS	40.0%
	AGCAAAGACAAAGAGAAAGG+TGG	+	chr1.1:12619096-12619115	None:intergenic	40.0%
	AGCTACTAAGACTAACTCCT+TGG	-	chr1.1:12619314-12619333	MS.gene31709:CDS	40.0%
	ATACACTTGCAAGTGGAGTA+GGG	-	chr1.1:12619971-12619990	MS.gene31709:CDS	40.0%
	CAATTTCCACTAACCAAAGC+AGG	+	chr1.1:12620192-12620211	None:intergenic	40.0%
	CTGCTTTGGTTAGTGGAAAT+TGG	-	chr1.1:12620190-12620209	MS.gene31709:intron	40.0%
	CTTCTTCATTATCTCTCTGG+TGG	-	chr1.1:12619564-12619583	MS.gene31709:intron	40.0%
	GATCATTGGGTTTATTGGGT+TGG	-	chr1.1:12620216-12620235	MS.gene31709:intron	40.0%
	GGACTGATCATTGGGTTTAT+TGG	-	chr1.1:12620211-12620230	MS.gene31709:intron	40.0%
	GTACACTCAAGGAGTAGTTT+GGG	-	chr1.1:12619993-12620012	MS.gene31709:intron	40.0%
	GTGGAAATTGGACTGATCAT+TGG	-	chr1.1:12620202-12620221	MS.gene31709:intron	40.0%
	TCCTTTCTTTGGATCAACCA+TGG	+	chr1.1:12620061-12620080	None:intergenic	40.0%
	TCGTTCGTTCGATCCTATAT+TGG	-	chr1.1:12619505-12619524	MS.gene31709:intron	40.0%
!	ATGAACCCTGCAAGATCTTT+TGG	-	chr1.1:12620165-12620184	MS.gene31709:intron	40.0%
!	GGTTGTTCCTAACGCAATTT+TGG	+	chr1.1:12619021-12619040	None:intergenic	40.0%
!	TCATTATCTCTCTGGTGGAT+TGG	-	chr1.1:12619569-12619588	MS.gene31709:intron	40.0%
!	TGAACCCTGCAAGATCTTTT+GGG	-	chr1.1:12620166-12620185	MS.gene31709:intron	40.0%
!!	TATTGGGTTGGACCACTTAT+TGG	-	chr1.1:12620228-12620247	MS.gene31709:CDS	40.0%
	AACCTTAGTTGGGTTTGTGG+TGG	-	chr1.1:12620101-12620120	MS.gene31709:intron	45.0%
	ACCACAAACCCAACTAAGGT+TGG	+	chr1.1:12620102-12620121	None:intergenic	45.0%
	ACCTTAGTTGGGTTTGTGGT+GGG	-	chr1.1:12620102-12620121	MS.gene31709:intron	45.0%
	CAGCTGCAGATGCTATCAAT+TGG	+	chr1.1:12619536-12619555	None:intergenic	45.0%
	CATACACTTGCAAGTGGAGT+AGG	-	chr1.1:12619970-12619989	MS.gene31709:CDS	45.0%
	CCACAAACCCAACTAAGGTT+GGG	+	chr1.1:12620101-12620120	None:intergenic	45.0%
	CTAAGTGGAGATGCCTTAGT+GGG	-	chr1.1:12619357-12619376	MS.gene31709:CDS	45.0%
	CTCTTTGTCTTTGCTGGTGT+TGG	-	chr1.1:12619101-12619120	MS.gene31709:CDS	45.0%
	GGTACACTCAAGGAGTAGTT+TGG	-	chr1.1:12619992-12620011	MS.gene31709:intron	45.0%
	GTTCACTGTGTATGCAACCA+TGG	-	chr1.1:12620041-12620060	MS.gene31709:intron	45.0%
	TAGTTATGTGACCTCCGACA+AGG	+	chr1.1:12619485-12619504	None:intergenic	45.0%
	TATTGCACATGCTCTTGTGG+TGG	-	chr1.1:12619395-12619414	MS.gene31709:intron	45.0%
	TCTTGCAGGGTTCATTGAAG+CGG	+	chr1.1:12620160-12620179	None:intergenic	45.0%
!	AAGATCTTTTGGGCCTGCTT+TGG	-	chr1.1:12620176-12620195	MS.gene31709:intron	45.0%
!!	AAATTTATTATTATTTATGT+TGG	-	chr1.1:12619779-12619798	MS.gene31709:CDS	5.0%
	AAGTCACGGCGGGATTCAAA+TGG	+	chr1.1:12619455-12619474	None:intergenic	50.0%
	AGTGGAGTAGGGTACACTCA+AGG	-	chr1.1:12619982-12620001	MS.gene31709:intron	50.0%
	ATCTCCGCCGCTCATATTTC+CGG	-	chr1.1:12619426-12619445	MS.gene31709:intron	50.0%
	ATGGCCACCGGAAATATGAG+CGG	+	chr1.1:12619436-12619455	None:intergenic	50.0%
	CAGGCCCAAAAGATCTTGCA+GGG	+	chr1.1:12620173-12620192	None:intergenic	50.0%
	CCACTTGCAAGTGTATGAGC+TGG	+	chr1.1:12619967-12619986	None:intergenic	50.0%
	CCAGCAAGTGCTCCTTTCTT+TGG	+	chr1.1:12620072-12620091	None:intergenic	50.0%
	CCAGCTCATACACTTGCAAG+TGG	-	chr1.1:12619964-12619983	MS.gene31709:CDS	50.0%
	CCCAACCTTAGTTGGGTTTG+TGG	-	chr1.1:12620098-12620117	MS.gene31709:intron	50.0%
	CCTTAGTTGGGTTTGTGGTG+GGG	-	chr1.1:12620103-12620122	MS.gene31709:intron	50.0%
	CTTAGTTGGGTTTGTGGTGG+GGG	-	chr1.1:12620104-12620123	MS.gene31709:intron	50.0%
	GCAAGTCCACCACCAATAAG+TGG	+	chr1.1:12620243-12620262	None:intergenic	50.0%
	GCTAAGTGGAGATGCCTTAG+TGG	-	chr1.1:12619356-12619375	MS.gene31709:CDS	50.0%
	GGCTATTGCACATGCTCTTG+TGG	-	chr1.1:12619392-12619411	MS.gene31709:intron	50.0%
	GTTGGACCACTTATTGGTGG+TGG	-	chr1.1:12620234-12620253	MS.gene31709:CDS	50.0%
	TGCGTTAGGAACAACCAGAG+AGG	-	chr1.1:12619025-12619044	MS.gene31709:CDS	50.0%
	TTATGTGACCTCCGACAAGG+AGG	+	chr1.1:12619482-12619501	None:intergenic	50.0%
	TTGAATCCCGCCGTGACTTT+GGG	-	chr1.1:12619456-12619475	MS.gene31709:intron	50.0%
	TTTGAATCCCGCCGTGACTT+TGG	-	chr1.1:12619455-12619474	MS.gene31709:intron	50.0%
	TTTGGGCCTGCTTTGGTTAG+TGG	-	chr1.1:12620183-12620202	MS.gene31709:intron	50.0%
!	TGGGTTGGACCACTTATTGG+TGG	-	chr1.1:12620231-12620250	MS.gene31709:CDS	50.0%
!!	CCAAAGAAAGGAGCACTTGC+TGG	-	chr1.1:12620069-12620088	MS.gene31709:intron	50.0%
!!	CTTATTGGTGGTGGACTTGC+TGG	-	chr1.1:12620243-12620262	MS.gene31709:CDS	50.0%
!!	GTTGGTTCTGCAATGACTGC+TGG	-	chr1.1:12619119-12619138	MS.gene31709:CDS	50.0%
	ATCAGGCTGAGTAGCCTCTC+TGG	+	chr1.1:12619042-12619061	None:intergenic	55.0%
	CCCCACCACAAACCCAACTA+AGG	+	chr1.1:12620106-12620125	None:intergenic	55.0%
	GACAAGGAGGCCCAAAGTCA+CGG	+	chr1.1:12619469-12619488	None:intergenic	55.0%
	GCAGGCCCAAAAGATCTTGC+AGG	+	chr1.1:12620174-12620193	None:intergenic	55.0%
	GGCTAACATCCTTGCTGGTG+GGG	-	chr1.1:12620125-12620144	MS.gene31709:intron	55.0%
	GGGCTAACATCCTTGCTGGT+GGG	-	chr1.1:12620124-12620143	MS.gene31709:intron	55.0%
	GTGACTTTGGGCCTCCTTGT+CGG	-	chr1.1:12619468-12619487	MS.gene31709:intron	55.0%
!	CCTTGCTGGTGGGGCTTTTT+CGG	-	chr1.1:12620134-12620153	MS.gene31709:intron	55.0%
!!	AAAGGAGCACTTGCTGGCCT+GGG	-	chr1.1:12620075-12620094	MS.gene31709:intron	55.0%
	AAGGAGGCCCAAAGTCACGG+CGG	+	chr1.1:12619466-12619485	None:intergenic	60.0%
	ACCCAACTAAGGTTGGGCCC+AGG	+	chr1.1:12620095-12620114	None:intergenic	60.0%
	ACTTTGGGCCTCCTTGTCGG+AGG	-	chr1.1:12619471-12619490	MS.gene31709:intron	60.0%
	CCGAAAAAGCCCCACCAGCA+AGG	+	chr1.1:12620137-12620156	None:intergenic	60.0%
	GCCACCGGAAATATGAGCGG+CGG	+	chr1.1:12619433-12619452	None:intergenic	60.0%
	GCCTGGGCCCAACCTTAGTT+GGG	-	chr1.1:12620091-12620110	MS.gene31709:intron	60.0%
	GGCGGGATTCAAATGGCCAC+CGG	+	chr1.1:12619448-12619467	None:intergenic	60.0%
	GGGGCTAACATCCTTGCTGG+TGG	-	chr1.1:12620123-12620142	MS.gene31709:intron	60.0%
	GTGGGGGCTAACATCCTTGC+TGG	-	chr1.1:12620120-12620139	MS.gene31709:intron	60.0%
	TCCGCCGCTCATATTTCCGG+TGG	-	chr1.1:12619429-12619448	MS.gene31709:intron	60.0%
!!	GAAAGGAGCACTTGCTGGCC+TGG	-	chr1.1:12620074-12620093	MS.gene31709:intron	60.0%
	AGGAGGCCCAAAGTCACGGC+GGG	+	chr1.1:12619465-12619484	None:intergenic	65.0%
	GGCCTGGGCCCAACCTTAGT+TGG	-	chr1.1:12620090-12620109	MS.gene31709:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	12619014	12620335	12619014	ID=MS.gene31709
chr1.1	mRNA	12619014	12620335	12619014	ID=MS.gene31709.t1;Parent=MS.gene31709
chr1.1	exon	12620227	12620335	12620227	ID=MS.gene31709.t1.exon1;Parent=MS.gene31709.t1
chr1.1	CDS	12620227	12620335	12620227	ID=cds.MS.gene31709.t1;Parent=MS.gene31709.t1
chr1.1	exon	12619759	12619997	12619759	ID=MS.gene31709.t1.exon2;Parent=MS.gene31709.t1
chr1.1	CDS	12619759	12619997	12619759	ID=cds.MS.gene31709.t1;Parent=MS.gene31709.t1
chr1.1	exon	12619014	12619391	12619014	ID=MS.gene31709.t1.exon3;Parent=MS.gene31709.t1
chr1.1	CDS	12619014	12619391	12619014	ID=cds.MS.gene31709.t1;Parent=MS.gene31709.t1

Gene Sequence

>MS.gene31709

ATGGCCAAAATTGCGTTAGGAACAACCAGAGAGGCTACTCAGCCTGATTGCATTCAAGCTCTAATTGTTGAATTCATTGCCACCTTTCTCTTTGTCTTTGCTGGTGTTGGTTCTGCAATGACTGCTGGTATATATATATATATATATATCTATACTATAACACTTATTTTTGTTATTATAACAATAACATTGTTACACATGAACTGATTCCTCATCCATTAATTTCATCCCATGTTATTTGAATTGAAATGTCATTCAATTTTCAATGTAATTTTACTAAATGTTCATAATTCAAACTCTAGCTACTAAGACTAACTCCTTGGAATTTATTTCAATAGATAAGCTAAGTGGAGATGCCTTAGTGGGATTGTTTTTTGTGGCTATTGCACATGCTCTTGTGGTGGCTGTAATGATCTCCGCCGCTCATATTTCCGGTGGCCATTTGAATCCCGCCGTGACTTTGGGCCTCCTTGTCGGAGGTCACATAACTATCGTTCGTTCGATCCTATATTGGATTGACCAATTGATAGCATCTGCAGCTGCTTGTTATCTTCTTCATTATCTCTCTGGTGGATTGGTAAGCTCTCAAATCTTCATATTTTTTTTACTTGCCAAAAATTACCACTTTTTTTAGGTTACAAATCATCATTTTTAAGGTACAAAAAATTGGGTTGAGACTAAAATTGTAATAAATGTAATATTTGAGGACCAAAACAATTTGATTTTAAAAGGAGGAAACTATTTTTGGAAGATTAAAATGCAATTAAATTTATTATTATTTATGTTGGAAATATCCTTATCCATACAATAAAATTGAAAAATACTTATCGATACAAATATTCTTGCTTTTTAAAGGGAAAAAGATAATAACCTTTTTTTGTCTTTTATTGTTGGAAAGATATAACTAAAAAAATATTGTACATTGAATTTTGCATGAATTACAGACAACTCCAGCTCATACACTTGCAAGTGGAGTAGGGTACACTCAAGGAGTAGTTTGGGAGATTGTGTTGACATTCTCTTTGTTGTTCACTGTGTATGCAACCATGGTTGATCCAAAGAAAGGAGCACTTGCTGGCCTGGGCCCAACCTTAGTTGGGTTTGTGGTGGGGGCTAACATCCTTGCTGGTGGGGCTTTTTCGGCCGCTTCAATGAACCCTGCAAGATCTTTTGGGCCTGCTTTGGTTAGTGGAAATTGGACTGATCATTGGGTTTATTGGGTTGGACCACTTATTGGTGGTGGACTTGCTGGTTTCATTTATGAGAATTTCTTCATTAATAGAGATCATGTTCCTCTAGTTGTAGATGAAGAAAGCTACTAA

Protein sequence

>MS.gene31709.t1

MAKIALGTTREATQPDCIQALIVEFIATFLFVFAGVDKLSGDALVGLFFVAIAHALVVAVMISAAHISGGHLNPAVTLGLLVGGHITIVRSILYWIDQLIASAAACYLLHYLSGGLTTPAHTLASGVGYTQGVVWEIVLTFSLLFTVYATMVDPKKGALAGLGPTLVGFVVGANILAGGAFSAASMNPARSFGPALVSGNWTDHWVYWVGPLIGGGLAGFIYENFFINRDHVPLVVDEESY