AlfalfaGEDB | MS.gene32445

M. sativa cultivar XinJiangDaYe / MS.gene32445

KEGG: K09873
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0015267 GO:0016020 GO:0022803 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00230.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32445.t1	XP_003588369.2	99.2	247	2	0	1	247	1	247	1.30E-111	412.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32445.t1	O82316	80.0	245	49	0	1	245	1	245	1.5e-90	334.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32445.t1	G7IB33	99.2	247	2	0	1	247	1	247	9.7e-112	412.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051749	MS.gene32445	0.828305	9.68E-55	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene32445	MS.gene01627	PPI
MS.gene32445	MS.gene019314	PPI
MS.gene32445	MS.gene042849	PPI
MS.gene32445	MS.gene002639	PPI
MS.gene32445	MS.gene062190	PPI
MS.gene07481	MS.gene32445	PPI
MS.gene32445	MS.gene004321	PPI
MS.gene32445	MS.gene72129	PPI
MS.gene32445	MS.gene61127	PPI
MS.gene32445	MS.gene27852	PPI
MS.gene32445	MS.gene75156	PPI
MS.gene32445	MS.gene90854	PPI
MS.gene32445	MS.gene03121	PPI
MS.gene32445	MS.gene93201	PPI
MS.gene32445	MS.gene024637	PPI
MS.gene32445	MS.gene004953	PPI
MS.gene32445	MS.gene71137	PPI
MS.gene32445	MS.gene34032	PPI
MS.gene32445	MS.gene54792	PPI
MS.gene32445	MS.gene49432	PPI
MS.gene32445	MS.gene67362	PPI
MS.gene32445	MS.gene72127	PPI
MS.gene32445	MS.gene002640	PPI
MS.gene32445	MS.gene048817	PPI
MS.gene32445	MS.gene069812	PPI
MS.gene32445	MS.gene79774	PPI
MS.gene32445	MS.gene03094	PPI
MS.gene32445	MS.gene92450	PPI
MS.gene32445	MS.gene01625	PPI
MS.gene32445	MS.gene032497	PPI
MS.gene32445	MS.gene89899	PPI
MS.gene32445	MS.gene065973	PPI
MS.gene32445	MS.gene072777	PPI
MS.gene32445	MS.gene004324	PPI
MS.gene32445	MS.gene004088	PPI
MS.gene32445	MS.gene021061	PPI
MS.gene32445	MS.gene03090	PPI
MS.gene32445	MS.gene052743	PPI
MS.gene32445	MS.gene014206	PPI
MS.gene32445	MS.gene025449	PPI
MS.gene32445	MS.gene21620	PPI
MS.gene32445	MS.gene33486	PPI
MS.gene32445	MS.gene061432	PPI
MS.gene32445	MS.gene27853	PPI
MS.gene32445	MS.gene023622	PPI
MS.gene32445	MS.gene03093	PPI
MS.gene32445	MS.gene025056	PPI
MS.gene32445	MS.gene037437	PPI
MS.gene32445	MS.gene002643	PPI
MS.gene32445	MS.gene83316	PPI
MS.gene32445	MS.gene005974	PPI
MS.gene32445	MS.gene065972	PPI
MS.gene32445	MS.gene01624	PPI
MS.gene32445	MS.gene34830	PPI
MS.gene32445	MS.gene004322	PPI
MS.gene32445	MS.gene023621	PPI
MS.gene32445	MS.gene029129	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32445.t1	MTR_1g006490	99.190	247	2	0	1	247	1	247	1.50e-177	488
MS.gene32445.t1	MTR_1g006590	85.830	247	35	0	1	247	1	247	4.18e-156	433
MS.gene32445.t1	MTR_8g013680	57.258	248	100	3	1	243	1	247	9.04e-91	268
MS.gene32445.t1	MTR_2g101370	56.557	244	102	3	4	243	5	248	8.04e-89	263
MS.gene32445.t1	MTR_4g063090	53.586	237	106	2	1	233	3	239	1.57e-80	242
MS.gene32445.t1	MTR_7g103030	53.689	244	108	3	1	240	3	245	4.33e-79	238
MS.gene32445.t1	MTR_1g115405	50.833	240	113	3	3	238	5	243	1.25e-78	237
MS.gene32445.t1	MTR_7g023350	53.498	243	108	3	3	240	5	247	5.42e-78	236
MS.gene32445.t1	MTR_5g012810	53.012	249	109	4	1	244	1	246	1.60e-77	234
MS.gene32445.t1	MTR_1g077540	43.874	253	131	3	3	244	5	257	1.43e-69	214
MS.gene32445.t1	MTR_6g069040	44.800	250	130	3	3	244	5	254	3.85e-67	208
MS.gene32445.t1	MTR_2g060360	38.000	250	147	4	1	243	1	249	1.83e-49	163
MS.gene32445.t1	MTR_5g063930	34.874	238	142	7	14	247	32	260	2.65e-33	121
MS.gene32445.t1	MTR_2g017620	36.052	233	140	6	18	247	23	249	4.27e-31	115
MS.gene32445.t1	MTR_2g017620	36.052	233	140	6	18	247	37	263	8.80e-31	115
MS.gene32445.t1	MTR_7g101190	33.878	245	141	7	9	234	29	271	2.35e-30	114
MS.gene32445.t1	MTR_2g094270	34.000	250	131	10	11	234	32	273	7.37e-30	113
MS.gene32445.t1	MTR_4g006730	36.585	205	110	7	24	220	80	272	2.18e-29	112
MS.gene32445.t1	MTR_3g118010	34.335	233	130	8	19	234	37	263	4.18e-29	111
MS.gene32445.t1	MTR_2g104940	37.073	205	109	7	24	220	85	277	5.69e-29	111
MS.gene32445.t1	MTR_1g097840	36.283	226	125	8	19	237	74	287	3.93e-28	108
MS.gene32445.t1	MTR_4g415300	35.088	228	126	7	19	229	56	278	7.20e-28	108
MS.gene32445.t1	MTR_5g010150	34.199	231	125	7	19	229	52	275	8.10e-28	107
MS.gene32445.t1	MTR_5g079570	36.087	230	138	7	18	245	39	261	1.22e-27	107
MS.gene32445.t1	MTR_2g017590	34.335	233	144	6	18	247	35	261	3.43e-27	105
MS.gene32445.t1	MTR_4g059390	33.992	253	128	12	10	234	30	271	3.81e-27	106
MS.gene32445.t1	MTR_4g006630	36.041	197	106	7	32	220	1	185	1.97e-26	102
MS.gene32445.t1	MTR_4g006730	36.041	197	106	7	32	220	1	185	2.17e-26	102
MS.gene32445.t1	MTR_8g098375	32.900	231	128	7	19	229	52	275	3.38e-26	103
MS.gene32445.t1	MTR_3g008920	36.866	217	128	7	18	232	25	234	3.41e-26	103
MS.gene32445.t1	MTR_2g017660	33.621	232	138	9	18	244	38	258	2.18e-25	101
MS.gene32445.t1	MTR_5g082070	35.135	222	125	7	19	224	55	273	3.07e-25	101
MS.gene32445.t1	MTR_3g070210	32.051	234	132	7	11	224	47	273	4.58e-25	100
MS.gene32445.t1	MTR_1g095070	34.737	190	100	6	62	235	24	205	3.28e-24	96.7
MS.gene32445.t1	MTR_7g010650	34.906	212	130	5	19	228	37	242	2.31e-23	95.9
MS.gene32445.t1	MTR_4g084880	34.091	220	128	7	18	229	34	244	3.76e-23	95.1
MS.gene32445.t1	MTR_8g087710	33.023	215	135	6	18	229	38	246	4.53e-23	95.1
MS.gene32445.t1	MTR_8g087720	32.558	215	136	6	18	229	37	245	1.30e-22	93.6
MS.gene32445.t1	MTR_2g017610	32.143	224	136	7	7	220	19	236	2.96e-22	92.8
MS.gene32445.t1	MTR_2g017620	35.359	181	111	4	69	247	6	182	5.06e-22	90.1
MS.gene32445.t1	MTR_1g070385	30.769	221	144	6	19	237	62	275	6.98e-22	94.7
MS.gene32445.t1	MTR_2g017570	31.696	224	137	7	7	220	19	236	1.32e-21	90.9
MS.gene32445.t1	MTR_2g094270	32.743	226	119	9	11	210	32	250	8.26e-21	89.0
MS.gene32445.t1	MTR_4g075570	27.863	262	156	8	10	247	42	294	3.47e-17	79.7
MS.gene32445.t1	MTR_4g075590	30.385	260	149	9	10	245	28	279	4.71e-17	79.3
MS.gene32445.t1	MTR_4g006730	35.882	170	89	7	24	185	80	237	6.40e-17	78.2
MS.gene32445.t1	MTR_3g070210	30.622	209	130	6	19	213	55	262	8.99e-17	77.8
MS.gene32445.t1	MTR_1g095070	32.727	165	89	5	62	210	24	182	3.80e-15	72.0
MS.gene32445.t1	MTR_1g097840	35.057	174	95	7	19	185	74	236	8.83e-15	72.4
MS.gene32445.t1	MTR_4g415300	34.615	156	87	5	19	164	56	206	5.47e-14	69.3
MS.gene32445.t1	MTR_5g010150	33.333	159	86	5	19	164	52	203	8.97e-14	68.6
MS.gene32445.t1	MTR_4g059390	33.523	176	86	9	10	164	30	195	2.91e-13	67.0
MS.gene32445.t1	MTR_8g098375	30.409	171	99	5	19	176	52	215	1.37e-12	65.5
MS.gene32445.t1	MTR_3g070210	29.560	159	92	5	19	164	55	206	1.60e-11	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32445.t1	AT2G25810	78.367	245	53	0	1	245	1	245	1.59e-132	374
MS.gene32445.t1	AT3G16240	56.098	246	101	4	1	240	1	245	4.77e-90	266
MS.gene32445.t1	AT2G36830	55.372	242	104	2	3	240	5	246	2.80e-89	265
MS.gene32445.t1	AT3G26520	54.701	234	102	2	7	236	10	243	3.93e-84	251
MS.gene32445.t1	AT5G47450	53.975	239	106	2	1	235	1	239	5.10e-83	248
MS.gene32445.t1	AT4G01470	51.012	247	116	3	3	244	5	251	3.35e-80	241
MS.gene32445.t1	AT4G17340	54.583	240	103	2	1	235	1	239	4.44e-79	238
MS.gene32445.t1	AT1G73190	48.249	257	115	3	6	244	11	267	1.10e-73	225
MS.gene32445.t1	AT1G17810	48.971	243	115	2	6	239	11	253	3.93e-70	216
MS.gene32445.t1	AT3G47440	42.009	219	122	3	16	230	20	237	8.92e-50	164
MS.gene32445.t1	AT2G37170	33.745	243	131	8	11	229	29	265	6.75e-31	115
MS.gene32445.t1	AT5G60660	34.746	236	132	8	19	235	39	271	7.72e-31	115
MS.gene32445.t1	AT2G37170	33.745	243	131	8	11	229	72	308	8.60e-31	116
MS.gene32445.t1	AT2G37180	34.440	241	134	8	10	229	28	265	1.04e-30	115
MS.gene32445.t1	AT4G35100	35.776	232	128	9	19	234	38	264	1.20e-30	115
MS.gene32445.t1	AT4G35100	35.776	232	128	9	19	234	38	264	1.20e-30	115
MS.gene32445.t1	AT3G54820	33.913	230	132	6	19	229	38	266	5.69e-30	113
MS.gene32445.t1	AT3G53420	34.348	230	131	7	19	229	39	267	2.50e-29	112
MS.gene32445.t1	AT3G53420	34.348	230	131	7	19	229	39	267	2.50e-29	112
MS.gene32445.t1	AT2G39010	33.473	239	139	7	10	229	29	266	4.25e-29	111
MS.gene32445.t1	AT1G80760	35.885	209	116	6	19	220	80	277	5.38e-29	111
MS.gene32445.t1	AT2G16850	34.483	232	131	9	19	234	36	262	1.24e-28	109
MS.gene32445.t1	AT4G00430	35.683	227	126	8	19	229	53	275	1.11e-27	107
MS.gene32445.t1	AT4G19030	35.747	221	128	7	18	229	54	269	2.20e-27	107
MS.gene32445.t1	AT4G10380	33.913	230	132	7	14	234	71	289	7.96e-27	105
MS.gene32445.t1	AT1G01620	34.783	230	124	8	19	229	52	274	8.44e-27	105
MS.gene32445.t1	AT4G23400	34.375	224	133	6	19	229	53	275	1.64e-26	104
MS.gene32445.t1	AT2G45960	36.000	225	118	8	19	224	52	269	4.28e-26	103
MS.gene32445.t1	AT3G61430	36.000	225	118	8	19	224	52	269	4.95e-26	103
MS.gene32445.t1	AT3G61430	36.000	225	118	8	19	224	52	269	4.95e-26	103
MS.gene32445.t1	AT5G37820	37.004	227	123	9	19	237	43	257	5.83e-26	102
MS.gene32445.t1	AT5G37820	36.522	230	125	9	19	240	79	295	9.66e-26	103
MS.gene32445.t1	AT4G18910	36.036	222	126	7	18	229	51	266	1.32e-25	102
MS.gene32445.t1	AT5G37810	35.371	229	126	8	12	229	33	250	1.24e-24	99.4
MS.gene32445.t1	AT1G31885	36.321	212	121	7	18	220	42	248	1.28e-24	100
MS.gene32445.t1	AT1G52180	50.000	86	43	0	125	210	39	124	5.51e-23	90.9
MS.gene32445.t1	AT1G01620	34.054	185	98	6	62	229	25	202	2.34e-22	91.7
MS.gene32445.t1	AT1G31885	35.176	199	115	7	31	220	1	194	1.21e-19	85.9
MS.gene32445.t1	AT2G45960	33.649	211	114	8	19	210	52	255	7.21e-18	80.9
MS.gene32445.t1	AT3G06100	30.374	214	144	5	18	230	45	254	7.24e-18	80.9
MS.gene32445.t1	AT2G45960	33.649	211	114	8	19	210	52	255	9.16e-18	80.9
MS.gene32445.t1	AT3G06100	31.250	208	138	5	24	230	3	206	1.83e-17	79.0
MS.gene32445.t1	AT2G34390	30.769	221	138	7	18	229	47	261	2.16e-17	79.7
MS.gene32445.t1	AT2G34390	30.734	218	136	7	21	229	8	219	1.68e-16	76.6
MS.gene32445.t1	AT2G39010	32.335	167	100	5	10	164	29	194	7.72e-14	68.9
MS.gene32445.t1	AT4G00430	35.484	155	87	6	19	164	53	203	9.16e-14	68.6
MS.gene32445.t1	AT4G19030	33.908	174	101	7	18	182	54	222	3.03e-13	67.4
MS.gene32445.t1	AT4G18910	34.831	178	100	7	18	185	51	222	5.63e-12	63.9
MS.gene32445.t1	AT4G19030	32.895	152	92	5	18	161	54	203	6.17e-12	63.5

Find 82 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAGCAAGTGCTCCTTTCTT+TGG	0.194627	1.4:+12337377	None:intergenic
ACCTTTCTCTTTGTCTTTGC+TGG	0.204008	1.4:-12338529	MS.gene32445:CDS
GGACTGATCATTGGGTTTAT+TGG	0.222150	1.4:-12337235	MS.gene32445:CDS
ATGAACCCTGCAAGATCTTT+TGG	0.224227	1.4:-12337281	MS.gene32445:CDS
CTGCTTTGGTTAGTGGAAAT+TGG	0.234876	1.4:-12337256	MS.gene32445:CDS
GGTTGTTCCTAACGCAATTT+TGG	0.235450	1.4:+12338606	None:intergenic
TATTGGGTTGGACCACTTAT+TGG	0.235959	1.4:-12337218	MS.gene32445:CDS
GGCCTTGGCCCAACCTTAGT+TGG	0.243998	1.4:-12337356	MS.gene32445:CDS
CTCTTTGTCTTTGCTGGTGT+TGG	0.290840	1.4:-12338523	MS.gene32445:CDS
CATACACTTGCAAGTGGAAT+AGG	0.300234	1.4:-12337476	MS.gene32445:CDS
TTGAATCCCGCCGTGACTTT+GGG	0.304849	1.4:-12337986	MS.gene32445:CDS
TTTGAATCCCGCCGTGACTT+TGG	0.311719	1.4:-12337987	MS.gene32445:CDS
GACTGATCATTGGGTTTATT+GGG	0.313313	1.4:-12337234	MS.gene32445:CDS
CTTATTGGTGGTGGACTTGC+TGG	0.315006	1.4:-12337203	MS.gene32445:CDS
TTTGGGCCTGCTTTGGTTAG+TGG	0.316883	1.4:-12337263	MS.gene32445:CDS
CTTAGTTGGGTTTGTGGTGG+GGG	0.319883	1.4:-12337342	MS.gene32445:CDS
ACCTTAGTTGGGTTTGTGGT+GGG	0.325499	1.4:-12337344	MS.gene32445:CDS
ATCTCCGCCGCTCATATTTC+CGG	0.326476	1.4:-12338016	MS.gene32445:CDS
GGGGCTAACATCCTTGCTGG+TGG	0.334049	1.4:-12337323	MS.gene32445:CDS
TCGTTCGTTCGATCCTATAT+TGG	0.337543	1.4:-12337937	MS.gene32445:CDS
TATCTTCTTCATTATCTCTC+TGG	0.355133	1.4:-12337881	MS.gene32445:CDS
GCCTTGGCCCAACCTTAGTT+GGG	0.359318	1.4:-12337355	MS.gene32445:CDS
GGGCTAACATCCTTGCTGGT+GGG	0.361838	1.4:-12337322	MS.gene32445:CDS
GGTACACTCAAGGAGTAGTT+TGG	0.361980	1.4:-12337454	MS.gene32445:CDS
CCCAACCTTAGTTGGGTTTG+TGG	0.373101	1.4:-12337348	MS.gene32445:CDS
GTGGAAATTGGACTGATCAT+TGG	0.389374	1.4:-12337244	MS.gene32445:CDS
TCAATTGGTCAATCCAATAT+AGG	0.391166	1.4:+12337924	None:intergenic
AAAGGAGCACTTGCTGGCCT+TGG	0.398295	1.4:-12337371	MS.gene32445:CDS
TGGAAATTGGACTGATCATT+GGG	0.400779	1.4:-12337243	MS.gene32445:CDS
ATCAGGCTGAGTGGCCTCTC+TGG	0.400862	1.4:+12338585	None:intergenic
TCATTATCTCTCTGGTGGAT+TGG	0.401572	1.4:-12337873	MS.gene32445:intron
CCAAAGAAAGGAGCACTTGC+TGG	0.403446	1.4:-12337377	MS.gene32445:CDS
GTGACTTTGGGCCTCCTTGT+CGG	0.411926	1.4:-12337974	MS.gene32445:CDS
CCTTAGTTGGGTTTGTGGTG+GGG	0.413230	1.4:-12337343	MS.gene32445:CDS
GGCTAACATCCTTGCTGGTG+GGG	0.419242	1.4:-12337321	MS.gene32445:CDS
AACCTTAGTTGGGTTTGTGG+TGG	0.423963	1.4:-12337345	MS.gene32445:CDS
GCTAAGTGGAGATGCCTTAG+TGG	0.435457	1.4:-12338086	MS.gene32445:CDS
GCAGGCCCAAAAGATCTTGC+AGG	0.436765	1.4:+12337275	None:intergenic
GATCATTGGGTTTATTGGGT+TGG	0.446033	1.4:-12337230	MS.gene32445:CDS
GTTGGACCACTTATTGGTGG+TGG	0.458421	1.4:-12337212	MS.gene32445:CDS
CTTCTTCATTATCTCTCTGG+TGG	0.466813	1.4:-12337878	MS.gene32445:CDS
ATACACTTGCAAGTGGAATA+GGG	0.488187	1.4:-12337475	MS.gene32445:CDS
GTTGGTTCTGCAATGACTGC+TGG	0.491784	1.4:-12338505	MS.gene32445:intron
AAGTCACGGCGGGATTCAAA+TGG	0.491922	1.4:+12337990	None:intergenic
GTACACTCAAGGAGTAGTTT+GGG	0.493061	1.4:-12337453	MS.gene32445:CDS
TGGGTTGGACCACTTATTGG+TGG	0.496312	1.4:-12337215	MS.gene32445:CDS
ACCACAAACCCAACTAAGGT+TGG	0.497042	1.4:+12337347	None:intergenic
GGCTATTGCACATGCTCTTG+TGG	0.499860	1.4:-12338050	MS.gene32445:CDS
CAGCTGCAGATGCTATCAAT+TGG	0.500751	1.4:+12337909	None:intergenic
ATTAGAGCTTGAATGCAATC+AGG	0.503619	1.4:+12338568	None:intergenic
CCACAAACCCAACTAAGGTT+GGG	0.515362	1.4:+12337348	None:intergenic
CCCCACCACAAACCCAACTA+AGG	0.515431	1.4:+12337343	None:intergenic
TCCTTTCTTTGGATCAACCA+TGG	0.517654	1.4:+12337388	None:intergenic
GACAAGGAGGCCCAAAGTCA+CGG	0.542123	1.4:+12337976	None:intergenic
ATTTCAATAGATAAGCTAAG+TGG	0.542575	1.4:-12338100	MS.gene32445:intron
ACCATGGTTGATCCAAAGAA+AGG	0.549142	1.4:-12337389	MS.gene32445:CDS
GCAAGTCCACCACCAATAAG+TGG	0.551655	1.4:+12337206	None:intergenic
CAATTTCCACTAACCAAAGC+AGG	0.552637	1.4:+12337257	None:intergenic
AGGAGGCCCAAAGTCACGGC+GGG	0.557990	1.4:+12337980	None:intergenic
ACCATGGCCAAAATTGCGTT+AGG	0.559940	1.4:-12338613	MS.gene32445:CDS
ACCAGCAAAGACAAAGAGAA+AGG	0.561490	1.4:+12338528	None:intergenic
CCACTTGCAAGTGTATGAGC+TGG	0.571448	1.4:+12337482	None:intergenic
CCAGCTCATACACTTGCAAG+TGG	0.572996	1.4:-12337482	MS.gene32445:CDS
CTTTCTTCATCTACAACTAG+AGG	0.584705	1.4:+12337140	None:intergenic
CTAAGTGGAGATGCCTTAGT+GGG	0.595922	1.4:-12338085	MS.gene32445:CDS
GGCGGGATTCAAATGGCCAC+CGG	0.596177	1.4:+12337997	None:intergenic
ACTTTGGGCCTCCTTGTCGG+AGG	0.600598	1.4:-12337971	MS.gene32445:CDS
TCTTGCAGGGTTCATTGAAG+CGG	0.607315	1.4:+12337289	None:intergenic
GTTCACTGTGTATGCAACCA+TGG	0.609878	1.4:-12337405	MS.gene32445:CDS
CAGGCCCAAAAGATCTTGCA+GGG	0.619708	1.4:+12337276	None:intergenic
ACCCAACTAAGGTTGGGCCA+AGG	0.626848	1.4:+12337354	None:intergenic
TATTGCACATGCTCTTGTGG+TGG	0.628052	1.4:-12338047	MS.gene32445:CDS
TCCGCCGCTCATATTTCCGG+TGG	0.641948	1.4:-12338013	MS.gene32445:CDS
TTGAATGCAATCAGGCTGAG+TGG	0.647396	1.4:+12338576	None:intergenic
AGCAAAGACAAAGAGAAAGG+TGG	0.654285	1.4:+12338531	None:intergenic
TAGTTATGTGACCTCCGACA+AGG	0.657530	1.4:+12337960	None:intergenic
AGTGGAATAGGGTACACTCA+AGG	0.668556	1.4:-12337464	MS.gene32445:CDS
GCCACCGGAAATATGAGCGG+CGG	0.678011	1.4:+12338012	None:intergenic
TGCGTTAGGAACAACCAGAG+AGG	0.706593	1.4:-12338599	MS.gene32445:CDS
ATGGCCACCGGAAATATGAG+CGG	0.716960	1.4:+12338009	None:intergenic
TTATGTGACCTCCGACAAGG+AGG	0.729011	1.4:+12337963	None:intergenic
AAGGAGGCCCAAAGTCACGG+CGG	0.732640	1.4:+12337979	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCTATTGAAATAAATTTCA+TGG	+	chr1.4:12337635-12337654	None:intergenic	15.0%
!!	TCTTATAAATCAAATGTATT+TGG	-	chr1.4:12337556-12337575	MS.gene32445:intron	15.0%
!!!	AAATATTCTTGCTTTTTAAA+GGG	-	chr1.4:12338145-12338164	MS.gene32445:intron	15.0%
!!!	ATTTTTCAGGTAAAAAAATT+TGG	-	chr1.4:12337958-12337977	MS.gene32445:CDS	15.0%
!!!	CAAAACAATTTGATTTTAAA+AGG	-	chr1.4:12338020-12338039	MS.gene32445:CDS	15.0%
!!!	CTTTTAAAATCAAATTGTTT+TGG	+	chr1.4:12338022-12338041	None:intergenic	15.0%
!!!	TAAAAATTACCACTTTTTTT+AGG	-	chr1.4:12337923-12337942	MS.gene32445:CDS	15.0%
!!!	TATTTTTCAATTTCATTGTA+TGG	+	chr1.4:12338114-12338133	None:intergenic	15.0%
!!!	TTTTTCAGGTAAAAAAATTT+GGG	-	chr1.4:12337959-12337978	MS.gene32445:CDS	15.0%
!!!	TTTTTTTTTCAATTAACTAG+TGG	-	chr1.4:12337296-12337315	MS.gene32445:CDS	15.0%
!!	ACATTTCAATTCAAATAACA+TGG	+	chr1.4:12337448-12337467	None:intergenic	20.0%
!!	CAACAATAAAAGACAAAAAA+AGG	+	chr1.4:12338184-12338203	None:intergenic	20.0%
!!	CCTAATGAAATATAAGAAAT+GGG	-	chr1.4:12337532-12337551	MS.gene32445:intron	20.0%
!!	CTAATGAAATATAAGAAATG+GGG	-	chr1.4:12337533-12337552	MS.gene32445:intron	20.0%
!!	TCCTAATGAAATATAAGAAA+TGG	-	chr1.4:12337531-12337550	MS.gene32445:intron	20.0%
!!	TGTAATAAACGTAATATTTG+AGG	-	chr1.4:12337995-12338014	MS.gene32445:CDS	20.0%
!!!	AACAATTTGATTTTAAAAGG+AGG	-	chr1.4:12338023-12338042	MS.gene32445:CDS	20.0%
!!!	AGTCAGTTAAATTAATCTTT+TGG	-	chr1.4:12337584-12337603	MS.gene32445:intron	20.0%
!!!	CAAATATTCTTGCTTTTTAA+AGG	-	chr1.4:12338144-12338163	MS.gene32445:intron	20.0%
!!!	CTTTTTTTGTCTTTTATTGT+TGG	-	chr1.4:12338182-12338201	MS.gene32445:intron	20.0%
!!!	GATTTTTAACCTAAAAAAAG+TGG	+	chr1.4:12337935-12337954	None:intergenic	20.0%
!!!	GTTAAAAATCATCATTTTTC+AGG	-	chr1.4:12337945-12337964	MS.gene32445:CDS	20.0%
!	ATTTCAATAGATAAGCTAAG+TGG	-	chr1.4:12337643-12337662	MS.gene32445:intron	25.0%
!	CCCATTTCTTATATTTCATT+AGG	+	chr1.4:12337535-12337554	None:intergenic	25.0%
!	TCAACTCTTCATATATTACT+TGG	-	chr1.4:12337900-12337919	MS.gene32445:CDS	25.0%
!	TCAATTTCATTGTATGGATA+AGG	+	chr1.4:12338108-12338127	None:intergenic	25.0%
!!	TAAAAGGAGGAAACTATTTT+TGG	-	chr1.4:12338036-12338055	MS.gene32445:CDS	25.0%
!!!	TTAATCTTTTGGAGAAAGTA+TGG	-	chr1.4:12337595-12337614	MS.gene32445:intron	25.0%
	TATCTTCTTCATTATCTCTC+TGG	-	chr1.4:12337862-12337881	MS.gene32445:intron	30.0%
	TCAATTGGTCAATCCAATAT+AGG	+	chr1.4:12337822-12337841	None:intergenic	30.0%
!!!	CTTTTTCAAGGTGAATAAGT+GGG	-	chr1.4:12337330-12337349	MS.gene32445:CDS	30.0%
	ATACACTTGCAAGTGGAATA+GGG	-	chr1.4:12338268-12338287	MS.gene32445:intron	35.0%
	ATTAGAGCTTGAATGCAATC+AGG	+	chr1.4:12337178-12337197	None:intergenic	35.0%
	CACAAAAAACAATCCCACTA+AGG	+	chr1.4:12337674-12337693	None:intergenic	35.0%
	CCACTTATTCACCTTGAAAA+AGG	+	chr1.4:12337332-12337351	None:intergenic	35.0%
	CTTTCTTCATCTACAACTAG+AGG	+	chr1.4:12338606-12338625	None:intergenic	35.0%
	GACATGCATTGTTATATGCA+GGG	+	chr1.4:12337377-12337396	None:intergenic	35.0%
	GACTGATCATTGGGTTTATT+GGG	-	chr1.4:12338509-12338528	MS.gene32445:CDS	35.0%
	TGGAAATTGGACTGATCATT+GGG	-	chr1.4:12338500-12338519	MS.gene32445:intron	35.0%
!!	GCTAGAATTCACCTTTTTCA+AGG	-	chr1.4:12337318-12337337	MS.gene32445:CDS	35.0%
!!!	CCTTTTTCAAGGTGAATAAG+TGG	-	chr1.4:12337329-12337348	MS.gene32445:CDS	35.0%
!!!	CTTAGTGGGATTGTTTTTTG+TGG	-	chr1.4:12337672-12337691	MS.gene32445:intron	35.0%
	ACCAGCAAAGACAAAGAGAA+AGG	+	chr1.4:12337218-12337237	None:intergenic	40.0%
	ACCATGGTTGATCCAAAGAA+AGG	-	chr1.4:12338354-12338373	MS.gene32445:intron	40.0%
	ACCTTTCTCTTTGTCTTTGC+TGG	-	chr1.4:12337214-12337233	MS.gene32445:CDS	40.0%
	AGCAAAGACAAAGAGAAAGG+TGG	+	chr1.4:12337215-12337234	None:intergenic	40.0%
	CAATTTCCACTAACCAAAGC+AGG	+	chr1.4:12338489-12338508	None:intergenic	40.0%
	CATACACTTGCAAGTGGAAT+AGG	-	chr1.4:12338267-12338286	MS.gene32445:intron	40.0%
	CTGCTTTGGTTAGTGGAAAT+TGG	-	chr1.4:12338487-12338506	MS.gene32445:intron	40.0%
	CTTCTTCATTATCTCTCTGG+TGG	-	chr1.4:12337865-12337884	MS.gene32445:intron	40.0%
	GATCATTGGGTTTATTGGGT+TGG	-	chr1.4:12338513-12338532	MS.gene32445:CDS	40.0%
	GGACATGCATTGTTATATGC+AGG	+	chr1.4:12337378-12337397	None:intergenic	40.0%
	GGACTGATCATTGGGTTTAT+TGG	-	chr1.4:12338508-12338527	MS.gene32445:CDS	40.0%
	GTACACTCAAGGAGTAGTTT+GGG	-	chr1.4:12338290-12338309	MS.gene32445:intron	40.0%
	GTGGAAATTGGACTGATCAT+TGG	-	chr1.4:12338499-12338518	MS.gene32445:intron	40.0%
	TCCTTTCTTTGGATCAACCA+TGG	+	chr1.4:12338358-12338377	None:intergenic	40.0%
	TCGTTCGTTCGATCCTATAT+TGG	-	chr1.4:12337806-12337825	MS.gene32445:intron	40.0%
	TGAGCATAACTCAGTTGACA+GGG	+	chr1.4:12337399-12337418	None:intergenic	40.0%
	TGCATTGTTATATGCAGGGT+CGG	+	chr1.4:12337373-12337392	None:intergenic	40.0%
!	ATGAACCCTGCAAGATCTTT+TGG	-	chr1.4:12338462-12338481	MS.gene32445:intron	40.0%
!	GGTTGTTCCTAACGCAATTT+TGG	+	chr1.4:12337140-12337159	None:intergenic	40.0%
!	TCATTATCTCTCTGGTGGAT+TGG	-	chr1.4:12337870-12337889	MS.gene32445:intron	40.0%
!	TGAACCCTGCAAGATCTTTT+GGG	-	chr1.4:12338463-12338482	MS.gene32445:intron	40.0%
!!	TATTGGGTTGGACCACTTAT+TGG	-	chr1.4:12338525-12338544	MS.gene32445:CDS	40.0%
	AACCTTAGTTGGGTTTGTGG+TGG	-	chr1.4:12338398-12338417	MS.gene32445:intron	45.0%
	ACCACAAACCCAACTAAGGT+TGG	+	chr1.4:12338399-12338418	None:intergenic	45.0%
	ACCTTAGTTGGGTTTGTGGT+GGG	-	chr1.4:12338399-12338418	MS.gene32445:intron	45.0%
	AGTGGAATAGGGTACACTCA+AGG	-	chr1.4:12338279-12338298	MS.gene32445:intron	45.0%
	CAGCTGCAGATGCTATCAAT+TGG	+	chr1.4:12337837-12337856	None:intergenic	45.0%
	CCACAAACCCAACTAAGGTT+GGG	+	chr1.4:12338398-12338417	None:intergenic	45.0%
	CTAAGTGGAGATGCCTTAGT+GGG	-	chr1.4:12337658-12337677	MS.gene32445:intron	45.0%
	CTCTTTGTCTTTGCTGGTGT+TGG	-	chr1.4:12337220-12337239	MS.gene32445:CDS	45.0%
	GCATTGTTATATGCAGGGTC+GGG	+	chr1.4:12337372-12337391	None:intergenic	45.0%
	GGTACACTCAAGGAGTAGTT+TGG	-	chr1.4:12338289-12338308	MS.gene32445:intron	45.0%
	GTGAGCATAACTCAGTTGAC+AGG	+	chr1.4:12337400-12337419	None:intergenic	45.0%
	GTTCACTGTGTATGCAACCA+TGG	-	chr1.4:12338338-12338357	MS.gene32445:intron	45.0%
	TAGTTATGTGACCTCCGACA+AGG	+	chr1.4:12337786-12337805	None:intergenic	45.0%
	TATTGCACATGCTCTTGTGG+TGG	-	chr1.4:12337696-12337715	MS.gene32445:intron	45.0%
	TCTTGCAGGGTTCATTGAAG+CGG	+	chr1.4:12338457-12338476	None:intergenic	45.0%
	TTGAATGCAATCAGGCTGAG+TGG	+	chr1.4:12337170-12337189	None:intergenic	45.0%
!	AAGATCTTTTGGGCCTGCTT+TGG	-	chr1.4:12338473-12338492	MS.gene32445:intron	45.0%
!!	GTGAATAAGTGGGTTGTCCA+GGG	-	chr1.4:12337340-12337359	MS.gene32445:CDS	45.0%
!!	AAATTTATTATTATTTATGT+TGG	-	chr1.4:12338076-12338095	MS.gene32445:CDS	5.0%
	AAGTCACGGCGGGATTCAAA+TGG	+	chr1.4:12337756-12337775	None:intergenic	50.0%
	ATCTCCGCCGCTCATATTTC+CGG	-	chr1.4:12337727-12337746	MS.gene32445:intron	50.0%
	ATGGCCACCGGAAATATGAG+CGG	+	chr1.4:12337737-12337756	None:intergenic	50.0%
	CAGGCCCAAAAGATCTTGCA+GGG	+	chr1.4:12338470-12338489	None:intergenic	50.0%
	CCACTTGCAAGTGTATGAGC+TGG	+	chr1.4:12338264-12338283	None:intergenic	50.0%
	CCAGCAAGTGCTCCTTTCTT+TGG	+	chr1.4:12338369-12338388	None:intergenic	50.0%
	CCAGCTCATACACTTGCAAG+TGG	-	chr1.4:12338261-12338280	MS.gene32445:intron	50.0%
	CCCAACCTTAGTTGGGTTTG+TGG	-	chr1.4:12338395-12338414	MS.gene32445:intron	50.0%
	CCTTAGTTGGGTTTGTGGTG+GGG	-	chr1.4:12338400-12338419	MS.gene32445:intron	50.0%
	CTTAGTTGGGTTTGTGGTGG+GGG	-	chr1.4:12338401-12338420	MS.gene32445:intron	50.0%
	GCAAGTCCACCACCAATAAG+TGG	+	chr1.4:12338540-12338559	None:intergenic	50.0%
	GCTAAGTGGAGATGCCTTAG+TGG	-	chr1.4:12337657-12337676	MS.gene32445:intron	50.0%
	GGCTATTGCACATGCTCTTG+TGG	-	chr1.4:12337693-12337712	MS.gene32445:intron	50.0%
	GTTGGACCACTTATTGGTGG+TGG	-	chr1.4:12338531-12338550	MS.gene32445:CDS	50.0%
	TGCGTTAGGAACAACCAGAG+AGG	-	chr1.4:12337144-12337163	MS.gene32445:CDS	50.0%
	TTATGTGACCTCCGACAAGG+AGG	+	chr1.4:12337783-12337802	None:intergenic	50.0%
	TTGAATCCCGCCGTGACTTT+GGG	-	chr1.4:12337757-12337776	MS.gene32445:intron	50.0%
	TTTGAATCCCGCCGTGACTT+TGG	-	chr1.4:12337756-12337775	MS.gene32445:intron	50.0%
	TTTGGGCCTGCTTTGGTTAG+TGG	-	chr1.4:12338480-12338499	MS.gene32445:intron	50.0%
!	TGGGTTGGACCACTTATTGG+TGG	-	chr1.4:12338528-12338547	MS.gene32445:CDS	50.0%
!!	CCAAAGAAAGGAGCACTTGC+TGG	-	chr1.4:12338366-12338385	MS.gene32445:intron	50.0%
!!	CTTATTGGTGGTGGACTTGC+TGG	-	chr1.4:12338540-12338559	MS.gene32445:CDS	50.0%
!!	GGTGAATAAGTGGGTTGTCC+AGG	-	chr1.4:12337339-12337358	MS.gene32445:CDS	50.0%
!!	GTTGGTTCTGCAATGACTGC+TGG	-	chr1.4:12337238-12337257	MS.gene32445:CDS	50.0%
	ACCCAACTAAGGTTGGGCCA+AGG	+	chr1.4:12338392-12338411	None:intergenic	55.0%
	CCCCACCACAAACCCAACTA+AGG	+	chr1.4:12338403-12338422	None:intergenic	55.0%
	GACAAGGAGGCCCAAAGTCA+CGG	+	chr1.4:12337770-12337789	None:intergenic	55.0%
	GCAGGCCCAAAAGATCTTGC+AGG	+	chr1.4:12338471-12338490	None:intergenic	55.0%
	GCCTTGGCCCAACCTTAGTT+GGG	-	chr1.4:12338388-12338407	MS.gene32445:intron	55.0%
	GGCTAACATCCTTGCTGGTG+GGG	-	chr1.4:12338422-12338441	MS.gene32445:intron	55.0%
	GGGCTAACATCCTTGCTGGT+GGG	-	chr1.4:12338421-12338440	MS.gene32445:intron	55.0%
	GTGACTTTGGGCCTCCTTGT+CGG	-	chr1.4:12337769-12337788	MS.gene32445:intron	55.0%
!	CCTTGCTGGTGGGGCTTTTT+CGG	-	chr1.4:12338431-12338450	MS.gene32445:intron	55.0%
!!	AAAGGAGCACTTGCTGGCCT+TGG	-	chr1.4:12338372-12338391	MS.gene32445:intron	55.0%
	AAGGAGGCCCAAAGTCACGG+CGG	+	chr1.4:12337767-12337786	None:intergenic	60.0%
	ACTTTGGGCCTCCTTGTCGG+AGG	-	chr1.4:12337772-12337791	MS.gene32445:intron	60.0%
	ATCAGGCTGAGTGGCCTCTC+TGG	+	chr1.4:12337161-12337180	None:intergenic	60.0%
	CCGAAAAAGCCCCACCAGCA+AGG	+	chr1.4:12338434-12338453	None:intergenic	60.0%
	GCCACCGGAAATATGAGCGG+CGG	+	chr1.4:12337734-12337753	None:intergenic	60.0%
	GGCCTTGGCCCAACCTTAGT+TGG	-	chr1.4:12338387-12338406	MS.gene32445:intron	60.0%
	GGCGGGATTCAAATGGCCAC+CGG	+	chr1.4:12337749-12337768	None:intergenic	60.0%
	GGGGCTAACATCCTTGCTGG+TGG	-	chr1.4:12338420-12338439	MS.gene32445:intron	60.0%
	GTGGGGGCTAACATCCTTGC+TGG	-	chr1.4:12338417-12338436	MS.gene32445:intron	60.0%
	TCCGCCGCTCATATTTCCGG+TGG	-	chr1.4:12337730-12337749	MS.gene32445:intron	60.0%
	AGGAGGCCCAAAGTCACGGC+GGG	+	chr1.4:12337766-12337785	None:intergenic	65.0%
	GCAGGGTCGGGTTCGAACCC+TGG	+	chr1.4:12337360-12337379	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	12337133	12338632	12337133	ID=MS.gene32445
chr1.4	mRNA	12337133	12338632	12337133	ID=MS.gene32445.t1;Parent=MS.gene32445
chr1.4	exon	12338506	12338632	12338506	ID=MS.gene32445.t1.exon1;Parent=MS.gene32445.t1
chr1.4	CDS	12338506	12338632	12338506	ID=cds.MS.gene32445.t1;Parent=MS.gene32445.t1
chr1.4	exon	12337874	12338112	12337874	ID=MS.gene32445.t1.exon2;Parent=MS.gene32445.t1
chr1.4	CDS	12337874	12338112	12337874	ID=cds.MS.gene32445.t1;Parent=MS.gene32445.t1
chr1.4	exon	12337133	12337510	12337133	ID=MS.gene32445.t1.exon3;Parent=MS.gene32445.t1
chr1.4	CDS	12337133	12337510	12337133	ID=cds.MS.gene32445.t1;Parent=MS.gene32445.t1

Gene Sequence

>MS.gene32445

ATGGCCAAAATTGCGTTAGGAACAACCAGAGAGGCCACTCAGCCTGATTGCATTCAAGCTCTAATTGTTGAATTCATTGCCACCTTTCTCTTTGTCTTTGCTGGTGTTGGTTCTGCAATGACTGCTGGTATATATATCTATACTATAACACTTATTTTCAGTTTTTTTTTTTCAATTAACTAGTGGCTAGAATTCACCTTTTTCAAGGTGAATAAGTGGGTTGTCCAGGGTTCGAACCCGACCCTGCATATAACAATGCATGTCCCTGTCAACTGAGTTATGCTCACAGAGACTTATTTTCAGTTCTTTAAGCCATGTTATTTGAATTGAAATGTCATTCAATTTTCAATGTAATTTTACTAAATGTTCATAATTCAAACTCTGACTACTATGAATATTCCTAATGAAATATAAGAAATGGGGTCTTATAAATCAAATGTATTTGGTCTAGAGTCAGTTAAATTAATCTTTTGGAGAAAGTATGGATCTAAGAATAACTCCATGAAATTTATTTCAATAGATAAGCTAAGTGGAGATGCCTTAGTGGGATTGTTTTTTGTGGCTATTGCACATGCTCTTGTGGTGGCTGTAATGATCTCCGCCGCTCATATTTCCGGTGGCCATTTGAATCCCGCCGTGACTTTGGGCCTCCTTGTCGGAGGTCACATAACTATCGTTCGTTCGATCCTATATTGGATTGACCAATTGATAGCATCTGCAGCTGCTTGTTATCTTCTTCATTATCTCTCTGGTGGATTGGTAAGCTCTCAACTCTTCATATATTACTTGGTAAAAATTACCACTTTTTTTAGGTTAAAAATCATCATTTTTCAGGTAAAAAAATTTGGGTTGAGACTAAAACTGTAATAAACGTAATATTTGAGGTCCAAAACAATTTGATTTTAAAAGGAGGAAACTATTTTTGGAAGATTAAAATGCAATTAAATTTATTATTATTTATGTTGGAAATATCCTTATCCATACAATGAAATTGAAAAATACTTATTGATACAAATATTCTTGCTTTTTAAAGGGAAAAAGATAATAACCTTTTTTTGTCTTTTATTGTTGGAAAGATATAACTAAAAAAATATTGTACATTGAATTTTGCATGAATTACAGACAACTCCAGCTCATACACTTGCAAGTGGAATAGGGTACACTCAAGGAGTAGTTTGGGAGATTGTGTTGACATTCTCTTTGTTGTTCACTGTGTATGCAACCATGGTTGATCCAAAGAAAGGAGCACTTGCTGGCCTTGGCCCAACCTTAGTTGGGTTTGTGGTGGGGGCTAACATCCTTGCTGGTGGGGCTTTTTCGGCCGCTTCAATGAACCCTGCAAGATCTTTTGGGCCTGCTTTGGTTAGTGGAAATTGGACTGATCATTGGGTTTATTGGGTTGGACCACTTATTGGTGGTGGACTTGCTGGTTTCATTTATGAGAATTTCTTCATTAATAGAGATCATGTTCCTCTAGTTGTAGATGAAGAAAGCTACTAA

Protein sequence

>MS.gene32445.t1

MAKIALGTTREATQPDCIQALIVEFIATFLFVFAGVGSAMTADKLSGDALVGLFFVAIAHALVVAVMISAAHISGGHLNPAVTLGLLVGGHITIVRSILYWIDQLIASAAACYLLHYLSGGLTTPAHTLASGIGYTQGVVWEIVLTFSLLFTVYATMVDPKKGALAGLGPTLVGFVVGANILAGGAFSAASMNPARSFGPALVSGNWTDHWVYWVGPLIGGGLAGFIYENFFINRDHVPLVVDEESY