AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene021393

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021393.t1 MTR_1g006490 99.595 247 1 0 1 247 1 247 8.21e-178 488
MS.gene021393.t1 MTR_1g006590 86.235 247 34 0 1 247 1 247 2.88e-156 434
MS.gene021393.t1 MTR_8g013680 57.661 248 99 3 1 243 1 247 5.24e-91 269
MS.gene021393.t1 MTR_2g101370 56.967 244 101 3 4 243 5 248 5.79e-89 263
MS.gene021393.t1 MTR_4g063090 54.008 237 105 2 1 233 3 239 9.03e-81 243
MS.gene021393.t1 MTR_7g103030 54.098 244 107 3 1 240 3 245 3.37e-79 239
MS.gene021393.t1 MTR_1g115405 51.250 240 112 3 3 238 5 243 8.69e-79 238
MS.gene021393.t1 MTR_7g023350 53.086 243 109 3 3 240 5 247 8.57e-78 235
MS.gene021393.t1 MTR_5g012810 53.012 249 109 4 1 244 1 246 2.10e-77 234
MS.gene021393.t1 MTR_1g077540 44.269 253 130 3 3 244 5 257 8.98e-70 215
MS.gene021393.t1 MTR_6g069040 44.400 250 131 3 3 244 5 254 5.88e-67 207
MS.gene021393.t1 MTR_2g060360 38.000 250 147 4 1 243 1 249 2.19e-49 163
MS.gene021393.t1 MTR_5g063930 34.874 238 142 7 14 247 32 260 1.97e-33 122
MS.gene021393.t1 MTR_2g017620 36.052 233 140 6 18 247 23 249 5.69e-31 115
MS.gene021393.t1 MTR_2g017620 36.052 233 140 6 18 247 37 263 1.10e-30 115
MS.gene021393.t1 MTR_7g101190 33.878 245 141 7 9 234 29 271 2.35e-30 114
MS.gene021393.t1 MTR_2g094270 34.008 247 135 9 11 234 32 273 5.97e-30 113
MS.gene021393.t1 MTR_4g006730 37.073 205 109 7 24 220 80 272 1.21e-29 113
MS.gene021393.t1 MTR_3g118010 34.335 233 130 8 19 234 37 263 4.10e-29 111
MS.gene021393.t1 MTR_2g104940 37.073 205 109 7 24 220 85 277 4.77e-29 111
MS.gene021393.t1 MTR_1g097840 35.841 226 126 7 19 237 74 287 2.96e-28 109
MS.gene021393.t1 MTR_4g415300 35.088 228 126 7 19 229 56 278 6.16e-28 108
MS.gene021393.t1 MTR_5g079570 36.522 230 137 7 18 245 39 261 7.85e-28 107
MS.gene021393.t1 MTR_5g010150 34.199 231 125 7 19 229 52 275 1.14e-27 107
MS.gene021393.t1 MTR_2g017590 34.335 233 144 6 18 247 35 261 2.87e-27 105
MS.gene021393.t1 MTR_4g059390 33.992 253 128 12 10 234 30 271 3.54e-27 106
MS.gene021393.t1 MTR_4g006630 36.548 197 105 7 32 220 1 185 1.28e-26 103
MS.gene021393.t1 MTR_4g006730 36.548 197 105 7 32 220 1 185 1.35e-26 103
MS.gene021393.t1 MTR_3g008920 37.327 217 127 7 18 232 25 234 1.90e-26 103
MS.gene021393.t1 MTR_8g098375 32.900 231 128 7 19 229 52 275 4.16e-26 103
MS.gene021393.t1 MTR_2g017660 33.621 232 138 9 18 244 38 258 1.79e-25 101
MS.gene021393.t1 MTR_5g082070 35.135 222 125 7 19 224 55 273 2.74e-25 101
MS.gene021393.t1 MTR_3g070210 32.895 228 138 6 11 224 47 273 4.35e-25 100
MS.gene021393.t1 MTR_1g095070 34.737 190 100 6 62 235 24 205 3.05e-24 97.1
MS.gene021393.t1 MTR_7g010650 34.906 212 130 5 19 228 37 242 2.43e-23 95.9
MS.gene021393.t1 MTR_8g087710 33.023 215 135 6 18 229 38 246 3.54e-23 95.5
MS.gene021393.t1 MTR_4g084880 33.182 220 130 6 18 229 34 244 4.39e-23 95.1
MS.gene021393.t1 MTR_8g087720 32.558 215 136 6 18 229 37 245 9.65e-23 94.0
MS.gene021393.t1 MTR_2g017610 32.143 224 136 7 7 220 19 236 2.59e-22 92.8
MS.gene021393.t1 MTR_2g017620 35.359 181 111 4 69 247 6 182 6.30e-22 90.1
MS.gene021393.t1 MTR_1g070385 30.769 221 144 6 19 237 62 275 6.47e-22 94.7
MS.gene021393.t1 MTR_2g017570 31.696 224 137 7 7 220 19 236 1.38e-21 90.9
MS.gene021393.t1 MTR_2g094270 32.735 223 123 8 11 210 32 250 8.43e-21 88.6
MS.gene021393.t1 MTR_4g006730 36.471 170 88 7 24 185 80 237 4.27e-17 78.6
MS.gene021393.t1 MTR_4g075570 27.481 262 157 8 10 247 42 294 5.52e-17 79.3
MS.gene021393.t1 MTR_4g075590 30.000 260 150 9 10 245 28 279 8.61e-17 78.6
MS.gene021393.t1 MTR_3g070210 30.622 209 130 6 19 213 55 262 8.64e-17 78.2
MS.gene021393.t1 MTR_1g095070 32.727 165 89 5 62 210 24 182 3.91e-15 72.0
MS.gene021393.t1 MTR_1g097840 34.483 174 96 6 19 185 74 236 8.32e-15 72.4
MS.gene021393.t1 MTR_4g415300 34.615 156 87 5 19 164 56 206 5.58e-14 69.3
MS.gene021393.t1 MTR_5g010150 33.333 159 86 5 19 164 52 203 1.24e-13 68.2
MS.gene021393.t1 MTR_4g059390 33.523 176 86 9 10 164 30 195 3.35e-13 66.6
MS.gene021393.t1 MTR_8g098375 30.409 171 99 5 19 176 52 215 1.88e-12 65.1
MS.gene021393.t1 MTR_3g070210 30.719 153 98 4 19 164 55 206 1.81e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021393.t1 AT2G25810 78.776 245 52 0 1 245 1 245 8.06e-133 375
MS.gene021393.t1 AT3G16240 56.098 246 101 4 1 240 1 245 6.13e-90 266
MS.gene021393.t1 AT2G36830 55.328 244 105 2 1 240 3 246 1.67e-89 265
MS.gene021393.t1 AT3G26520 55.128 234 101 2 7 236 10 243 2.56e-84 252
MS.gene021393.t1 AT5G47450 53.975 239 106 2 1 235 1 239 6.69e-83 248
MS.gene021393.t1 AT4G01470 51.417 247 115 3 3 244 5 251 2.21e-80 242
MS.gene021393.t1 AT4G17340 54.583 240 103 2 1 235 1 239 6.36e-79 238
MS.gene021393.t1 AT1G73190 48.638 257 114 3 6 244 11 267 6.82e-74 226
MS.gene021393.t1 AT1G17810 49.383 243 114 2 6 239 11 253 2.74e-70 217
MS.gene021393.t1 AT3G47440 42.009 219 122 3 16 230 20 237 1.04e-49 164
MS.gene021393.t1 AT2G37170 33.745 243 131 8 11 229 29 265 6.75e-31 115
MS.gene021393.t1 AT5G60660 34.746 236 132 8 19 235 39 271 7.32e-31 116
MS.gene021393.t1 AT2G37170 33.745 243 131 8 11 229 72 308 7.43e-31 117
MS.gene021393.t1 AT4G35100 35.776 232 128 9 19 234 38 264 7.82e-31 115
MS.gene021393.t1 AT4G35100 35.776 232 128 9 19 234 38 264 7.82e-31 115
MS.gene021393.t1 AT2G37180 34.440 241 134 8 10 229 28 265 1.03e-30 115
MS.gene021393.t1 AT3G54820 33.913 230 132 6 19 229 38 266 5.82e-30 113
MS.gene021393.t1 AT3G53420 34.348 230 131 7 19 229 39 267 2.37e-29 112
MS.gene021393.t1 AT3G53420 34.348 230 131 7 19 229 39 267 2.37e-29 112
MS.gene021393.t1 AT1G80760 36.364 209 115 6 19 220 80 277 2.81e-29 112
MS.gene021393.t1 AT2G39010 33.473 239 139 7 10 229 29 266 4.30e-29 111
MS.gene021393.t1 AT2G16850 34.483 232 131 9 19 234 36 262 8.14e-29 110
MS.gene021393.t1 AT4G00430 35.683 227 126 8 19 229 53 275 6.96e-28 108
MS.gene021393.t1 AT4G19030 36.199 221 127 7 18 229 54 269 1.57e-27 107
MS.gene021393.t1 AT4G10380 33.913 230 132 7 14 234 71 289 7.41e-27 105
MS.gene021393.t1 AT1G01620 34.802 227 128 8 19 229 52 274 7.52e-27 105
MS.gene021393.t1 AT4G23400 34.375 224 133 6 19 229 53 275 1.43e-26 104
MS.gene021393.t1 AT2G45960 36.000 225 118 8 19 224 52 269 3.98e-26 103
MS.gene021393.t1 AT3G61430 36.000 225 118 8 19 224 52 269 4.41e-26 103
MS.gene021393.t1 AT3G61430 36.000 225 118 8 19 224 52 269 4.41e-26 103
MS.gene021393.t1 AT5G37820 37.004 227 123 9 19 237 43 257 6.88e-26 102
MS.gene021393.t1 AT5G37820 36.522 230 125 9 19 240 79 295 9.36e-26 103
MS.gene021393.t1 AT4G18910 36.036 222 126 7 18 229 51 266 1.13e-25 102
MS.gene021393.t1 AT1G31885 36.321 212 121 7 18 220 42 248 1.07e-24 100
MS.gene021393.t1 AT5G37810 35.371 229 126 8 12 229 33 250 1.21e-24 99.4
MS.gene021393.t1 AT1G52180 51.163 86 42 0 125 210 39 124 3.64e-23 91.3
MS.gene021393.t1 AT1G01620 34.066 182 102 6 62 229 25 202 2.02e-22 92.0
MS.gene021393.t1 AT1G31885 35.176 199 115 7 31 220 1 194 1.12e-19 85.9
MS.gene021393.t1 AT2G45960 33.649 211 114 8 19 210 52 255 7.29e-18 80.9
MS.gene021393.t1 AT3G06100 30.374 214 144 5 18 230 45 254 9.70e-18 80.5
MS.gene021393.t1 AT2G45960 33.649 211 114 8 19 210 52 255 1.15e-17 80.9
MS.gene021393.t1 AT2G34390 30.769 221 138 7 18 229 47 261 1.75e-17 80.1
MS.gene021393.t1 AT3G06100 31.250 208 138 5 24 230 3 206 2.72e-17 78.6
MS.gene021393.t1 AT2G34390 30.734 218 136 7 21 229 8 219 1.56e-16 76.6
MS.gene021393.t1 AT4G00430 35.484 155 87 6 19 164 53 203 6.51e-14 68.9
MS.gene021393.t1 AT2G39010 32.335 167 100 5 10 164 29 194 9.25e-14 68.9
MS.gene021393.t1 AT4G19030 34.483 174 100 7 18 182 54 222 1.84e-13 67.8
MS.gene021393.t1 AT4G19030 33.553 152 91 5 18 161 54 203 4.07e-12 64.3
MS.gene021393.t1 AT4G18910 34.831 178 100 7 18 185 51 222 6.09e-12 63.5

Find 82 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGCAAGTGCTCCTTTCTT+TGG 0.194627 1.2:+12498721 None:intergenic
ACCTTTCTCTTTGTCTTTGC+TGG 0.204008 1.2:-12499871 MS.gene021393:CDS
GGACTGATCATTGGGTTTAT+TGG 0.222150 1.2:-12498579 MS.gene021393:CDS
ATGAACCCTGCAAGATCTTT+TGG 0.224227 1.2:-12498625 MS.gene021393:CDS
CTGCTTTGGTTAGTGGAAAT+TGG 0.234876 1.2:-12498600 MS.gene021393:CDS
GGTTGTTCCTAACGCAATTT+TGG 0.235450 1.2:+12499948 None:intergenic
TATTGGGTTGGACCACTTAT+TGG 0.235959 1.2:-12498562 MS.gene021393:CDS
GGCCTTGGCCCAACCTTAGT+TGG 0.243998 1.2:-12498700 MS.gene021393:CDS
CTCTTTGTCTTTGCTGGTGT+TGG 0.290840 1.2:-12499865 MS.gene021393:CDS
TTGAATCCCGCCGTGACTTT+GGG 0.304849 1.2:-12499329 MS.gene021393:CDS
TTTGAATCCCGCCGTGACTT+TGG 0.311719 1.2:-12499330 MS.gene021393:CDS
GACTGATCATTGGGTTTATT+GGG 0.313313 1.2:-12498578 MS.gene021393:CDS
CTTATTGGTGGTGGACTTGC+TGG 0.315006 1.2:-12498547 MS.gene021393:CDS
TTTGGGCCTGCTTTGGTTAG+TGG 0.316883 1.2:-12498607 MS.gene021393:CDS
CTTAGTTGGGTTTGTGGTGG+GGG 0.319883 1.2:-12498686 MS.gene021393:CDS
ACCTTAGTTGGGTTTGTGGT+GGG 0.325499 1.2:-12498688 MS.gene021393:CDS
ATCTCCGCCGCTCATATTTC+CGG 0.326476 1.2:-12499359 MS.gene021393:CDS
GGGGCTAACATCCTTGCTGG+TGG 0.334049 1.2:-12498667 MS.gene021393:CDS
TCGTTCGTTCGATCCTATAT+TGG 0.337543 1.2:-12499280 MS.gene021393:CDS
TATCTTCTTCATTATCTCTC+TGG 0.355133 1.2:-12499224 MS.gene021393:CDS
GCCTTGGCCCAACCTTAGTT+GGG 0.359318 1.2:-12498699 MS.gene021393:CDS
GGGCTAACATCCTTGCTGGT+GGG 0.361838 1.2:-12498666 MS.gene021393:CDS
CCCAACCTTAGTTGGGTTTG+TGG 0.373101 1.2:-12498692 MS.gene021393:CDS
GGTACACTCAAGGAGTAGTT+TGG 0.375382 1.2:-12498798 MS.gene021393:CDS
GTGGAAATTGGACTGATCAT+TGG 0.389374 1.2:-12498588 MS.gene021393:CDS
TCAATTGGTCAATCCAATAT+AGG 0.391166 1.2:+12499267 None:intergenic
AAAGGAGCACTTGCTGGCCT+TGG 0.398295 1.2:-12498715 MS.gene021393:CDS
TGGAAATTGGACTGATCATT+GGG 0.400779 1.2:-12498587 MS.gene021393:CDS
ATCAGGCTGAGTGGCCTCTC+TGG 0.400862 1.2:+12499927 None:intergenic
TCATTATCTCTCTGGTGGAT+TGG 0.401572 1.2:-12499216 MS.gene021393:intron
CCAAAGAAAGGAGCACTTGC+TGG 0.403446 1.2:-12498721 MS.gene021393:CDS
GTGACTTTGGGCCTCCTTGT+CGG 0.411926 1.2:-12499317 MS.gene021393:CDS
CCTTAGTTGGGTTTGTGGTG+GGG 0.413230 1.2:-12498687 MS.gene021393:CDS
GGCTAACATCCTTGCTGGTG+GGG 0.419242 1.2:-12498665 MS.gene021393:CDS
AACCTTAGTTGGGTTTGTGG+TGG 0.423963 1.2:-12498689 MS.gene021393:CDS
GCTAAGTGGAGATGCCTTAG+TGG 0.435457 1.2:-12499429 MS.gene021393:CDS
GCAGGCCCAAAAGATCTTGC+AGG 0.436765 1.2:+12498619 None:intergenic
GATCATTGGGTTTATTGGGT+TGG 0.446033 1.2:-12498574 MS.gene021393:CDS
GTTGGACCACTTATTGGTGG+TGG 0.458421 1.2:-12498556 MS.gene021393:CDS
CTTCTTCATTATCTCTCTGG+TGG 0.466813 1.2:-12499221 MS.gene021393:CDS
CATACACTTGCAAGTGGAGT+AGG 0.467366 1.2:-12498820 MS.gene021393:CDS
GTTGGTTCTGCAATGACTGC+TGG 0.491784 1.2:-12499847 MS.gene021393:intron
AAGTCACGGCGGGATTCAAA+TGG 0.491922 1.2:+12499333 None:intergenic
GTACACTCAAGGAGTAGTTT+GGG 0.493061 1.2:-12498797 MS.gene021393:CDS
TGGGTTGGACCACTTATTGG+TGG 0.496312 1.2:-12498559 MS.gene021393:CDS
ACCACAAACCCAACTAAGGT+TGG 0.497042 1.2:+12498691 None:intergenic
GGCTATTGCACATGCTCTTG+TGG 0.499860 1.2:-12499393 MS.gene021393:CDS
CAGCTGCAGATGCTATCAAT+TGG 0.500751 1.2:+12499252 None:intergenic
ATTAGAGCTTGAATGCAATC+AGG 0.503619 1.2:+12499910 None:intergenic
CCACAAACCCAACTAAGGTT+GGG 0.515362 1.2:+12498692 None:intergenic
CCCCACCACAAACCCAACTA+AGG 0.515431 1.2:+12498687 None:intergenic
TCCTTTCTTTGGATCAACCA+TGG 0.517654 1.2:+12498732 None:intergenic
GACAAGGAGGCCCAAAGTCA+CGG 0.542123 1.2:+12499319 None:intergenic
ATTTCAATAGATAAGCTAAG+TGG 0.542575 1.2:-12499443 MS.gene021393:intron
ACCATGGTTGATCCAAAGAA+AGG 0.549142 1.2:-12498733 MS.gene021393:CDS
GCAAGTCCACCACCAATAAG+TGG 0.551655 1.2:+12498550 None:intergenic
CAATTTCCACTAACCAAAGC+AGG 0.552637 1.2:+12498601 None:intergenic
AGGAGGCCCAAAGTCACGGC+GGG 0.557990 1.2:+12499323 None:intergenic
ACCATGGCCAAAATTGCGTT+AGG 0.559940 1.2:-12499955 MS.gene021393:CDS
ACCAGCAAAGACAAAGAGAA+AGG 0.561490 1.2:+12499870 None:intergenic
CCACTTGCAAGTGTATGAGC+TGG 0.565802 1.2:+12498826 None:intergenic
CCAGCTCATACACTTGCAAG+TGG 0.572996 1.2:-12498826 MS.gene021393:CDS
CTTTCTTCATCTACAACTAG+AGG 0.584705 1.2:+12498484 None:intergenic
CTAAGTGGAGATGCCTTAGT+GGG 0.595922 1.2:-12499428 MS.gene021393:CDS
GGCGGGATTCAAATGGCCAC+CGG 0.596177 1.2:+12499340 None:intergenic
ACTTTGGGCCTCCTTGTCGG+AGG 0.600598 1.2:-12499314 MS.gene021393:CDS
ATACACTTGCAAGTGGAGTA+GGG 0.603588 1.2:-12498819 MS.gene021393:CDS
TCTTGCAGGGTTCATTGAAG+CGG 0.607315 1.2:+12498633 None:intergenic
GTTCACTGTGTATGCAACCA+TGG 0.609878 1.2:-12498749 MS.gene021393:CDS
CAGGCCCAAAAGATCTTGCA+GGG 0.619708 1.2:+12498620 None:intergenic
ACCCAACTAAGGTTGGGCCA+AGG 0.626848 1.2:+12498698 None:intergenic
TATTGCACATGCTCTTGTGG+TGG 0.628052 1.2:-12499390 MS.gene021393:CDS
TCCGCCGCTCATATTTCCGG+TGG 0.641948 1.2:-12499356 MS.gene021393:CDS
TTGAATGCAATCAGGCTGAG+TGG 0.647396 1.2:+12499918 None:intergenic
AGCAAAGACAAAGAGAAAGG+TGG 0.654285 1.2:+12499873 None:intergenic
TAGTTATGTGACCTCCGACA+AGG 0.657530 1.2:+12499303 None:intergenic
AGTGGAGTAGGGTACACTCA+AGG 0.668556 1.2:-12498808 MS.gene021393:CDS
GCCACCGGAAATATGAGCGG+CGG 0.678011 1.2:+12499355 None:intergenic
TGCGTTAGGAACAACCAGAG+AGG 0.706593 1.2:-12499941 MS.gene021393:CDS
ATGGCCACCGGAAATATGAG+CGG 0.716960 1.2:+12499352 None:intergenic
TTATGTGACCTCCGACAAGG+AGG 0.729011 1.2:+12499306 None:intergenic
AAGGAGGCCCAAAGTCACGG+CGG 0.732640 1.2:+12499322 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATAAATTTAATTTATTT+TGG + chr1.2:12499409-12499428 None:intergenic 0.0%
!!! TATTTTTCAATTTTATTGTA+TGG + chr1.2:12499456-12499475 None:intergenic 10.0%
!! ATCTATTGAAATAAATTTCA+TGG + chr1.2:12498978-12498997 None:intergenic 15.0%
!! TCTTATAAATCAAATGTATT+TGG - chr1.2:12498899-12498918 MS.gene021393:intron 15.0%
!!! AAATATTCTTGCTTTTTAAA+GGG - chr1.2:12499487-12499506 MS.gene021393:intron 15.0%
!!! ATTTTTAAGGTACAAAAATT+TGG - chr1.2:12499301-12499320 MS.gene021393:CDS 15.0%
!!! CAAAACAATTTGATTTTAAA+AGG - chr1.2:12499363-12499382 MS.gene021393:CDS 15.0%
!!! CTTTTAAAATCAAATTGTTT+TGG + chr1.2:12499365-12499384 None:intergenic 15.0%
!!! GTTATAAATCATCATTTTTA+AGG - chr1.2:12499288-12499307 MS.gene021393:CDS 15.0%
!!! TAAAAATTACCACTTTTTTT+AGG - chr1.2:12499266-12499285 MS.gene021393:CDS 15.0%
!!! TTTTTAAGGTACAAAAATTT+GGG - chr1.2:12499302-12499321 MS.gene021393:CDS 15.0%
!!! TTTTTTTTTCAATTAACTAG+TGG - chr1.2:12498639-12498658 MS.gene021393:CDS 15.0%
!! ACATTTCAATTCAAATAACA+TGG + chr1.2:12498791-12498810 None:intergenic 20.0%
!! CAACAATAAAAGACAAAAAA+AGG + chr1.2:12499526-12499545 None:intergenic 20.0%
!! CCTAATGAAATATAAGAAAT+GGG - chr1.2:12498875-12498894 MS.gene021393:intron 20.0%
!! CTAATGAAATATAAGAAATG+GGG - chr1.2:12498876-12498895 MS.gene021393:intron 20.0%
!! GATTTATAACCTAAAAAAAG+TGG + chr1.2:12499278-12499297 None:intergenic 20.0%
!! TCCTAATGAAATATAAGAAA+TGG - chr1.2:12498874-12498893 MS.gene021393:intron 20.0%
!!! AACAATTTGATTTTAAAAGG+AGG - chr1.2:12499366-12499385 MS.gene021393:CDS 20.0%
!!! AGTCAGTTAAATTAATCTTT+TGG - chr1.2:12498927-12498946 MS.gene021393:intron 20.0%
!!! CAAATATTCTTGCTTTTTAA+AGG - chr1.2:12499486-12499505 MS.gene021393:intron 20.0%
!!! CTTTTTTTGTCTTTTATTGT+TGG - chr1.2:12499524-12499543 MS.gene021393:intron 20.0%
!!! TCAATTTTATTGTATGGATA+AGG + chr1.2:12499450-12499469 None:intergenic 20.0%
! ATTTCAATAGATAAGCTAAG+TGG - chr1.2:12498986-12499005 MS.gene021393:intron 25.0%
! CCCATTTCTTATATTTCATT+AGG + chr1.2:12498878-12498897 None:intergenic 25.0%
! TCAACTCTTCATATATTACT+TGG - chr1.2:12499243-12499262 MS.gene021393:CDS 25.0%
!! TAAAAGGAGGAAACTATTTT+TGG - chr1.2:12499379-12499398 MS.gene021393:CDS 25.0%
!!! TTAATCTTTTGGAGAAAGTA+TGG - chr1.2:12498938-12498957 MS.gene021393:intron 25.0%
TATCTTCTTCATTATCTCTC+TGG - chr1.2:12499205-12499224 MS.gene021393:intron 30.0%
TCAATTGGTCAATCCAATAT+AGG + chr1.2:12499165-12499184 None:intergenic 30.0%
!!! CTTTTTCAAGGTGAATAAGT+GGG - chr1.2:12498673-12498692 MS.gene021393:CDS 30.0%
ATTAGAGCTTGAATGCAATC+AGG + chr1.2:12498522-12498541 None:intergenic 35.0%
CACAAAAAACAATCCCACTA+AGG + chr1.2:12499017-12499036 None:intergenic 35.0%
CCACTTATTCACCTTGAAAA+AGG + chr1.2:12498675-12498694 None:intergenic 35.0%
CTTTCTTCATCTACAACTAG+AGG + chr1.2:12499948-12499967 None:intergenic 35.0%
GACATGCATTGTTATATGCA+GGG + chr1.2:12498720-12498739 None:intergenic 35.0%
GACTGATCATTGGGTTTATT+GGG - chr1.2:12499851-12499870 MS.gene021393:CDS 35.0%
TGGAAATTGGACTGATCATT+GGG - chr1.2:12499842-12499861 MS.gene021393:intron 35.0%
!! GCTAGAATTCACCTTTTTCA+AGG - chr1.2:12498661-12498680 MS.gene021393:CDS 35.0%
!!! CCTTTTTCAAGGTGAATAAG+TGG - chr1.2:12498672-12498691 MS.gene021393:CDS 35.0%
!!! CTTAGTGGGATTGTTTTTTG+TGG - chr1.2:12499015-12499034 MS.gene021393:intron 35.0%
ACCAGCAAAGACAAAGAGAA+AGG + chr1.2:12498562-12498581 None:intergenic 40.0%
ACCATGGTTGATCCAAAGAA+AGG - chr1.2:12499696-12499715 MS.gene021393:intron 40.0%
ACCTTTCTCTTTGTCTTTGC+TGG - chr1.2:12498558-12498577 MS.gene021393:CDS 40.0%
AGCAAAGACAAAGAGAAAGG+TGG + chr1.2:12498559-12498578 None:intergenic 40.0%
ATACACTTGCAAGTGGAGTA+GGG - chr1.2:12499610-12499629 MS.gene021393:intron 40.0%
CAATTTCCACTAACCAAAGC+AGG + chr1.2:12499831-12499850 None:intergenic 40.0%
CTGCTTTGGTTAGTGGAAAT+TGG - chr1.2:12499829-12499848 MS.gene021393:intron 40.0%
CTTCTTCATTATCTCTCTGG+TGG - chr1.2:12499208-12499227 MS.gene021393:intron 40.0%
GATCATTGGGTTTATTGGGT+TGG - chr1.2:12499855-12499874 MS.gene021393:CDS 40.0%
GGACATGCATTGTTATATGC+AGG + chr1.2:12498721-12498740 None:intergenic 40.0%
GGACTGATCATTGGGTTTAT+TGG - chr1.2:12499850-12499869 MS.gene021393:CDS 40.0%
GTACACTCAAGGAGTAGTTT+GGG - chr1.2:12499632-12499651 MS.gene021393:intron 40.0%
GTGGAAATTGGACTGATCAT+TGG - chr1.2:12499841-12499860 MS.gene021393:intron 40.0%
TCCTTTCTTTGGATCAACCA+TGG + chr1.2:12499700-12499719 None:intergenic 40.0%
TCGTTCGTTCGATCCTATAT+TGG - chr1.2:12499149-12499168 MS.gene021393:intron 40.0%
TGAGCATAACTCAGTTGACA+GGG + chr1.2:12498742-12498761 None:intergenic 40.0%
TGCATTGTTATATGCAGGGT+CGG + chr1.2:12498716-12498735 None:intergenic 40.0%
! ATGAACCCTGCAAGATCTTT+TGG - chr1.2:12499804-12499823 MS.gene021393:intron 40.0%
! GGTTGTTCCTAACGCAATTT+TGG + chr1.2:12498484-12498503 None:intergenic 40.0%
! TCATTATCTCTCTGGTGGAT+TGG - chr1.2:12499213-12499232 MS.gene021393:intron 40.0%
! TGAACCCTGCAAGATCTTTT+GGG - chr1.2:12499805-12499824 MS.gene021393:intron 40.0%
!! TATTGGGTTGGACCACTTAT+TGG - chr1.2:12499867-12499886 MS.gene021393:CDS 40.0%
AACCTTAGTTGGGTTTGTGG+TGG - chr1.2:12499740-12499759 MS.gene021393:intron 45.0%
ACCACAAACCCAACTAAGGT+TGG + chr1.2:12499741-12499760 None:intergenic 45.0%
ACCTTAGTTGGGTTTGTGGT+GGG - chr1.2:12499741-12499760 MS.gene021393:intron 45.0%
CAGCTGCAGATGCTATCAAT+TGG + chr1.2:12499180-12499199 None:intergenic 45.0%
CATACACTTGCAAGTGGAGT+AGG - chr1.2:12499609-12499628 MS.gene021393:intron 45.0%
CCACAAACCCAACTAAGGTT+GGG + chr1.2:12499740-12499759 None:intergenic 45.0%
CTAAGTGGAGATGCCTTAGT+GGG - chr1.2:12499001-12499020 MS.gene021393:intron 45.0%
CTCTTTGTCTTTGCTGGTGT+TGG - chr1.2:12498564-12498583 MS.gene021393:CDS 45.0%
GCATTGTTATATGCAGGGTC+GGG + chr1.2:12498715-12498734 None:intergenic 45.0%
GGTACACTCAAGGAGTAGTT+TGG - chr1.2:12499631-12499650 MS.gene021393:intron 45.0%
GTGAGCATAACTCAGTTGAC+AGG + chr1.2:12498743-12498762 None:intergenic 45.0%
GTTCACTGTGTATGCAACCA+TGG - chr1.2:12499680-12499699 MS.gene021393:intron 45.0%
TAGTTATGTGACCTCCGACA+AGG + chr1.2:12499129-12499148 None:intergenic 45.0%
TATTGCACATGCTCTTGTGG+TGG - chr1.2:12499039-12499058 MS.gene021393:intron 45.0%
TCTTGCAGGGTTCATTGAAG+CGG + chr1.2:12499799-12499818 None:intergenic 45.0%
TTGAATGCAATCAGGCTGAG+TGG + chr1.2:12498514-12498533 None:intergenic 45.0%
! AAGATCTTTTGGGCCTGCTT+TGG - chr1.2:12499815-12499834 MS.gene021393:intron 45.0%
!! GTGAATAAGTGGGTTGTCCA+GGG - chr1.2:12498683-12498702 MS.gene021393:CDS 45.0%
!! AAATTTATTATTATTTATGT+TGG - chr1.2:12499418-12499437 MS.gene021393:CDS 5.0%
AAGTCACGGCGGGATTCAAA+TGG + chr1.2:12499099-12499118 None:intergenic 50.0%
AGTGGAGTAGGGTACACTCA+AGG - chr1.2:12499621-12499640 MS.gene021393:intron 50.0%
ATCTCCGCCGCTCATATTTC+CGG - chr1.2:12499070-12499089 MS.gene021393:intron 50.0%
ATGGCCACCGGAAATATGAG+CGG + chr1.2:12499080-12499099 None:intergenic 50.0%
CAGGCCCAAAAGATCTTGCA+GGG + chr1.2:12499812-12499831 None:intergenic 50.0%
CCACTTGCAAGTGTATGAGC+TGG + chr1.2:12499606-12499625 None:intergenic 50.0%
CCAGCAAGTGCTCCTTTCTT+TGG + chr1.2:12499711-12499730 None:intergenic 50.0%
CCAGCTCATACACTTGCAAG+TGG - chr1.2:12499603-12499622 MS.gene021393:intron 50.0%
CCCAACCTTAGTTGGGTTTG+TGG - chr1.2:12499737-12499756 MS.gene021393:intron 50.0%
CCTTAGTTGGGTTTGTGGTG+GGG - chr1.2:12499742-12499761 MS.gene021393:intron 50.0%
CTTAGTTGGGTTTGTGGTGG+GGG - chr1.2:12499743-12499762 MS.gene021393:intron 50.0%
GCAAGTCCACCACCAATAAG+TGG + chr1.2:12499882-12499901 None:intergenic 50.0%
GCTAAGTGGAGATGCCTTAG+TGG - chr1.2:12499000-12499019 MS.gene021393:intron 50.0%
GGCTATTGCACATGCTCTTG+TGG - chr1.2:12499036-12499055 MS.gene021393:intron 50.0%
GTTGGACCACTTATTGGTGG+TGG - chr1.2:12499873-12499892 MS.gene021393:CDS 50.0%
TGCGTTAGGAACAACCAGAG+AGG - chr1.2:12498488-12498507 MS.gene021393:CDS 50.0%
TTATGTGACCTCCGACAAGG+AGG + chr1.2:12499126-12499145 None:intergenic 50.0%
TTGAATCCCGCCGTGACTTT+GGG - chr1.2:12499100-12499119 MS.gene021393:intron 50.0%
TTTGAATCCCGCCGTGACTT+TGG - chr1.2:12499099-12499118 MS.gene021393:intron 50.0%
TTTGGGCCTGCTTTGGTTAG+TGG - chr1.2:12499822-12499841 MS.gene021393:intron 50.0%
! TGGGTTGGACCACTTATTGG+TGG - chr1.2:12499870-12499889 MS.gene021393:CDS 50.0%
!! CCAAAGAAAGGAGCACTTGC+TGG - chr1.2:12499708-12499727 MS.gene021393:intron 50.0%
!! CTTATTGGTGGTGGACTTGC+TGG - chr1.2:12499882-12499901 MS.gene021393:CDS 50.0%
!! GGTGAATAAGTGGGTTGTCC+AGG - chr1.2:12498682-12498701 MS.gene021393:CDS 50.0%
!! GTTGGTTCTGCAATGACTGC+TGG - chr1.2:12498582-12498601 MS.gene021393:CDS 50.0%
ACCCAACTAAGGTTGGGCCA+AGG + chr1.2:12499734-12499753 None:intergenic 55.0%
CCCCACCACAAACCCAACTA+AGG + chr1.2:12499745-12499764 None:intergenic 55.0%
GACAAGGAGGCCCAAAGTCA+CGG + chr1.2:12499113-12499132 None:intergenic 55.0%
GCAGGCCCAAAAGATCTTGC+AGG + chr1.2:12499813-12499832 None:intergenic 55.0%
GCCTTGGCCCAACCTTAGTT+GGG - chr1.2:12499730-12499749 MS.gene021393:intron 55.0%
GGCTAACATCCTTGCTGGTG+GGG - chr1.2:12499764-12499783 MS.gene021393:intron 55.0%
GGGCTAACATCCTTGCTGGT+GGG - chr1.2:12499763-12499782 MS.gene021393:intron 55.0%
GTGACTTTGGGCCTCCTTGT+CGG - chr1.2:12499112-12499131 MS.gene021393:intron 55.0%
! CCTTGCTGGTGGGGCTTTTT+CGG - chr1.2:12499773-12499792 MS.gene021393:intron 55.0%
!! AAAGGAGCACTTGCTGGCCT+TGG - chr1.2:12499714-12499733 MS.gene021393:intron 55.0%
AAGGAGGCCCAAAGTCACGG+CGG + chr1.2:12499110-12499129 None:intergenic 60.0%
ACTTTGGGCCTCCTTGTCGG+AGG - chr1.2:12499115-12499134 MS.gene021393:intron 60.0%
ATCAGGCTGAGTGGCCTCTC+TGG + chr1.2:12498505-12498524 None:intergenic 60.0%
CCGAAAAAGCCCCACCAGCA+AGG + chr1.2:12499776-12499795 None:intergenic 60.0%
GCCACCGGAAATATGAGCGG+CGG + chr1.2:12499077-12499096 None:intergenic 60.0%
GGCCTTGGCCCAACCTTAGT+TGG - chr1.2:12499729-12499748 MS.gene021393:intron 60.0%
GGCGGGATTCAAATGGCCAC+CGG + chr1.2:12499092-12499111 None:intergenic 60.0%
GGGGCTAACATCCTTGCTGG+TGG - chr1.2:12499762-12499781 MS.gene021393:intron 60.0%
GTGGGGGCTAACATCCTTGC+TGG - chr1.2:12499759-12499778 MS.gene021393:intron 60.0%
TCCGCCGCTCATATTTCCGG+TGG - chr1.2:12499073-12499092 MS.gene021393:intron 60.0%
AGGAGGCCCAAAGTCACGGC+GGG + chr1.2:12499109-12499128 None:intergenic 65.0%
GCAGGGTCGGGTTCGAACCC+TGG + chr1.2:12498703-12498722 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 12498477 12499974 12498477 ID=MS.gene021393
chr1.2 mRNA 12498477 12499974 12498477 ID=MS.gene021393.t1;Parent=MS.gene021393
chr1.2 exon 12499848 12499974 12499848 ID=MS.gene021393.t1.exon1;Parent=MS.gene021393.t1
chr1.2 CDS 12499848 12499974 12499848 ID=cds.MS.gene021393.t1;Parent=MS.gene021393.t1
chr1.2 exon 12499217 12499455 12499217 ID=MS.gene021393.t1.exon2;Parent=MS.gene021393.t1
chr1.2 CDS 12499217 12499455 12499217 ID=cds.MS.gene021393.t1;Parent=MS.gene021393.t1
chr1.2 exon 12498477 12498854 12498477 ID=MS.gene021393.t1.exon3;Parent=MS.gene021393.t1
chr1.2 CDS 12498477 12498854 12498477 ID=cds.MS.gene021393.t1;Parent=MS.gene021393.t1
Gene Sequence

>MS.gene021393

ATGGCCAAAATTGCGTTAGGAACAACCAGAGAGGCCACTCAGCCTGATTGCATTCAAGCTCTAATTGTTGAATTCATTGCCACCTTTCTCTTTGTCTTTGCTGGTGTTGGTTCTGCAATGACTGCTGGTATATATATCTATACTATAACACTTATTTTCAGTTTTTTTTTTCAATTAACTAGTGGCTAGAATTCACCTTTTTCAAGGTGAATAAGTGGGTTGTCCAGGGTTCGAACCCGACCCTGCATATAACAATGCATGTCCCTGTCAACTGAGTTATGCTCACAGAGACTTATTTTCAGTTCTTTAAGCCATGTTATTTGAATTGAAATGTCATTCAATTTTCAATGTAATTTTACTAAATGTTCATAATTCAAACTCTGACTACTATGAATATTCCTAATGAAATATAAGAAATGGGGTCTTATAAATCAAATGTATTTGGTCTAGAGTCAGTTAAATTAATCTTTTGGAGAAAGTATGGATCTAAGAATAACTCCATGAAATTTATTTCAATAGATAAGCTAAGTGGAGATGCCTTAGTGGGATTGTTTTTTGTGGCTATTGCACATGCTCTTGTGGTGGCTGTAATGATCTCCGCCGCTCATATTTCCGGTGGCCATTTGAATCCCGCCGTGACTTTGGGCCTCCTTGTCGGAGGTCACATAACTATCGTTCGTTCGATCCTATATTGGATTGACCAATTGATAGCATCTGCAGCTGCTTGTTATCTTCTTCATTATCTCTCTGGTGGATTGGTAAGCTCTCAACTCTTCATATATTACTTGGTAAAAATTACCACTTTTTTTAGGTTATAAATCATCATTTTTAAGGTACAAAAATTTGGGTTGAGACTAAAATTGTAATAAATGTAATATTTGAAGACCAAAACAATTTGATTTTAAAAGGAGGAAACTATTTTTGGAAGACCAAAATAAATTAAATTTATTATTATTTATGTTGGAAATATCCTTATCCATACAATAAAATTGAAAAATACTTATTGATACAAATATTCTTGCTTTTTAAAGGGAAAAAGATAATAACCTTTTTTTGTCTTTTATTGTTGGAAAGATATAACTAAAAAAATATTGTACATTGAATTTTGCATGAATTACAGACAACTCCAGCTCATACACTTGCAAGTGGAGTAGGGTACACTCAAGGAGTAGTTTGGGAGATTGTGTTGACATTCTCTTTGTTGTTCACTGTGTATGCAACCATGGTTGATCCAAAGAAAGGAGCACTTGCTGGCCTTGGCCCAACCTTAGTTGGGTTTGTGGTGGGGGCTAACATCCTTGCTGGTGGGGCTTTTTCGGCCGCTTCAATGAACCCTGCAAGATCTTTTGGGCCTGCTTTGGTTAGTGGAAATTGGACTGATCATTGGGTTTATTGGGTTGGACCACTTATTGGTGGTGGACTTGCTGGTTTCATTTATGAGAATTTCTTCATTAATAGAGATCATGTTCCTCTAGTTGTAGATGAAGAAAGCTACTAA
Protein sequence

>MS.gene021393.t1

MAKIALGTTREATQPDCIQALIVEFIATFLFVFAGVGSAMTADKLSGDALVGLFFVAIAHALVVAVMISAAHISGGHLNPAVTLGLLVGGHITIVRSILYWIDQLIASAAACYLLHYLSGGLTTPAHTLASGVGYTQGVVWEIVLTFSLLFTVYATMVDPKKGALAGLGPTLVGFVVGANILAGGAFSAASMNPARSFGPALVSGNWTDHWVYWVGPLIGGGLAGFIYENFFINRDHVPLVVDEESY