Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75434.t1 | XP_003602028.1 | 93.9 | 115 | 7 | 0 | 25 | 139 | 106 | 220 | 4.70E-53 | 217.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75434.t1 | G7J4F6 | 93.9 | 115 | 7 | 0 | 25 | 139 | 106 | 220 | 3.4e-53 | 217.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049178 | MS.gene75434 | 0.809467 | 1.87E-50 | -1.69E-46 |
| MS.gene049378 | MS.gene75434 | 0.804208 | 2.41E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene75434 | 0.800057 | 1.72E-48 | -1.69E-46 |
| MS.gene050719 | MS.gene75434 | 0.809969 | 1.45E-50 | -1.69E-46 |
| MS.gene050721 | MS.gene75434 | 0.809498 | 1.84E-50 | -1.69E-46 |
| MS.gene051063 | MS.gene75434 | 0.808565 | 2.91E-50 | -1.69E-46 |
| MS.gene051757 | MS.gene75434 | 0.801524 | 8.64E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene75434 | 0.827499 | 1.51E-54 | -1.69E-46 |
| MS.gene052302 | MS.gene75434 | 0.837956 | 3.83E-57 | -1.69E-46 |
| MS.gene052309 | MS.gene75434 | -0.80971 | 1.65E-50 | -1.69E-46 |
| MS.gene052358 | MS.gene75434 | -0.800614 | 1.33E-48 | -1.69E-46 |
| MS.gene052376 | MS.gene75434 | 0.849704 | 2.73E-60 | -1.69E-46 |
| MS.gene054930 | MS.gene75434 | 0.825442 | 4.68E-54 | -1.69E-46 |
| MS.gene056069 | MS.gene75434 | -0.81443 | 1.55E-51 | -1.69E-46 |
| MS.gene057967 | MS.gene75434 | 0.800711 | 1.27E-48 | -1.69E-46 |
| MS.gene058084 | MS.gene75434 | 0.819049 | 1.42E-52 | -1.69E-46 |
| MS.gene058182 | MS.gene75434 | 0.815534 | 8.80E-52 | -1.69E-46 |
| MS.gene058200 | MS.gene75434 | 0.806925 | 6.49E-50 | -1.69E-46 |
| MS.gene058906 | MS.gene75434 | 0.818717 | 1.69E-52 | -1.69E-46 |
| MS.gene059249 | MS.gene75434 | 0.802045 | 6.76E-49 | -1.69E-46 |
| MS.gene06001 | MS.gene75434 | 0.820666 | 6.08E-53 | -1.69E-46 |
| MS.gene060869 | MS.gene75434 | 0.822778 | 1.97E-53 | -1.69E-46 |
| MS.gene061040 | MS.gene75434 | -0.817368 | 3.42E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75434.t1 | MTR_3g088010 | 93.913 | 115 | 7 | 0 | 25 | 139 | 106 | 220 | 5.63e-66 | 199 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75434.t1 | AT1G21830 | 44.000 | 125 | 56 | 7 | 25 | 136 | 79 | 202 | 1.72e-22 | 88.6 |
Find 37 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATCAAGTTTGAAGGAAA+TGG | 0.311026 | 3.3:+73293721 | MS.gene75434:CDS |
| CAAAGTCACGTGACACATTC+AGG | 0.325911 | 3.3:+73293851 | MS.gene75434:CDS |
| CAATCAAGTTTGAAGGAAAT+GGG | 0.363983 | 3.3:+73293722 | MS.gene75434:CDS |
| CTCATAAGGTGGAAGAAAAC+TGG | 0.371691 | 3.3:+73293904 | MS.gene75434:CDS |
| GGTTCTTCTTGCAATTCTCT+AGG | 0.383703 | 3.3:-73293789 | None:intergenic |
| TGGTCTCTAATTTGTCCTCA+TGG | 0.391766 | 3.3:-73293810 | None:intergenic |
| TTCATATCAATCAAGTTTGA+AGG | 0.415927 | 3.3:+73293715 | MS.gene75434:CDS |
| GTCACATGTTCCAGCTCATA+AGG | 0.455731 | 3.3:+73293890 | MS.gene75434:CDS |
| CATTCTCATTTCTCATTCCA+AGG | 0.456313 | 3.3:-73293663 | None:intergenic |
| TGCATGCTCTATCACACTCT+TGG | 0.456919 | 3.3:-73293830 | None:intergenic |
| GTTCTTCTTGCAATTCTCTA+GGG | 0.470567 | 3.3:-73293788 | None:intergenic |
| CACGTAGGCAACAAGGTTGA+AGG | 0.495184 | 3.3:+73293937 | MS.gene75434:CDS |
| GTCTACTATACTTTATTCCA+TGG | 0.505676 | 3.3:-73294019 | None:intergenic |
| TCATTTCTCATTCCAAGGGT+AGG | 0.532456 | 3.3:-73293658 | None:intergenic |
| TTTGTGTGGGAACCTACCCT+TGG | 0.535888 | 3.3:+73293646 | MS.gene75434:CDS |
| CTTCTTGCAATTCTCTAGGG+AGG | 0.538057 | 3.3:-73293785 | None:intergenic |
| ACACATTCAGGTGGAGAAAG+AGG | 0.548112 | 3.3:+73293863 | MS.gene75434:CDS |
| CAGTGAAAGTCAGAAAAGGC+TGG | 0.555530 | 3.3:+73293965 | MS.gene75434:CDS |
| GGAGCAGTGAAAGTCAGAAA+AGG | 0.558648 | 3.3:+73293961 | MS.gene75434:CDS |
| CTTGCAATTCTCTAGGGAGG+AGG | 0.560179 | 3.3:-73293782 | None:intergenic |
| CTTCAACCTTGTTGCCTACG+TGG | 0.576338 | 3.3:-73293936 | None:intergenic |
| AAGTCAGAAAAGGCTGGATG+AGG | 0.577043 | 3.3:+73293971 | MS.gene75434:CDS |
| TTCAGGTGGAGAAAGAGGAT+TGG | 0.577306 | 3.3:+73293868 | MS.gene75434:CDS |
| GGAAAAGAGCACGGTCTCAA+AGG | 0.587379 | 3.3:+73293756 | MS.gene75434:CDS |
| ATTCTCATTTCTCATTCCAA+GGG | 0.589136 | 3.3:-73293662 | None:intergenic |
| AGGAAATGGGGTAGCCTCGT+GGG | 0.614477 | 3.3:+73293735 | MS.gene75434:CDS |
| TGTATGCCACGTAGGCAACA+AGG | 0.624880 | 3.3:+73293930 | MS.gene75434:CDS |
| AAGGAAATGGGGTAGCCTCG+TGG | 0.628547 | 3.3:+73293734 | MS.gene75434:CDS |
| ACATGTTCCAGCTCATAAGG+TGG | 0.637291 | 3.3:+73293893 | MS.gene75434:CDS |
| AATCAAGTTTGAAGGAAATG+GGG | 0.642471 | 3.3:+73293723 | MS.gene75434:CDS |
| AGCCTCGTGGGAAAAGAGCA+CGG | 0.642699 | 3.3:+73293747 | MS.gene75434:CDS |
| AAAGAGAAAGAAGAAGACCA+TGG | 0.647060 | 3.3:+73294002 | MS.gene75434:CDS |
| GTAGGCAACAAGGTTGAAGG+AGG | 0.670514 | 3.3:+73293940 | MS.gene75434:CDS |
| ACTGGAGGTGTATGCCACGT+AGG | 0.690562 | 3.3:+73293922 | MS.gene75434:CDS |
| ATAAGGTGGAAGAAAACTGG+AGG | 0.709203 | 3.3:+73293907 | MS.gene75434:CDS |
| AGTCACGTGACACATTCAGG+TGG | 0.713866 | 3.3:+73293854 | MS.gene75434:CDS |
| GAATTGCAAGAAGAACCATG+AGG | 0.821093 | 3.3:+73293795 | MS.gene75434:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCATATCAATCAAGTTTGA+AGG | + | chr3.3:73293715-73293734 | MS.gene75434:CDS | 25.0% |
| !!! | CTTGTCTATGTATTTTTACT+TGG | + | chr3.3:73293604-73293623 | MS.gene75434:intron | 25.0% |
| AATCAAGTTTGAAGGAAATG+GGG | + | chr3.3:73293723-73293742 | MS.gene75434:CDS | 30.0% | |
| ATTCTCATTTCTCATTCCAA+GGG | - | chr3.3:73293665-73293684 | None:intergenic | 30.0% | |
| CAATCAAGTTTGAAGGAAAT+GGG | + | chr3.3:73293722-73293741 | MS.gene75434:CDS | 30.0% | |
| TCAATCAAGTTTGAAGGAAA+TGG | + | chr3.3:73293721-73293740 | MS.gene75434:CDS | 30.0% | |
| ! | CATTAGATTTTGCTTTGTGT+GGG | + | chr3.3:73293633-73293652 | MS.gene75434:intron | 30.0% |
| ! | TCATTAGATTTTGCTTTGTG+TGG | + | chr3.3:73293632-73293651 | MS.gene75434:intron | 30.0% |
| AAAGAGAAAGAAGAAGACCA+TGG | + | chr3.3:73294002-73294021 | MS.gene75434:CDS | 35.0% | |
| CATTCTCATTTCTCATTCCA+AGG | - | chr3.3:73293666-73293685 | None:intergenic | 35.0% | |
| GTTCTTCTTGCAATTCTCTA+GGG | - | chr3.3:73293791-73293810 | None:intergenic | 35.0% | |
| ATAAGGTGGAAGAAAACTGG+AGG | + | chr3.3:73293907-73293926 | MS.gene75434:CDS | 40.0% | |
| CTCATAAGGTGGAAGAAAAC+TGG | + | chr3.3:73293904-73293923 | MS.gene75434:CDS | 40.0% | |
| GAATTGCAAGAAGAACCATG+AGG | + | chr3.3:73293795-73293814 | MS.gene75434:CDS | 40.0% | |
| GGTTCTTCTTGCAATTCTCT+AGG | - | chr3.3:73293792-73293811 | None:intergenic | 40.0% | |
| TCATTTCTCATTCCAAGGGT+AGG | - | chr3.3:73293661-73293680 | None:intergenic | 40.0% | |
| ! | TGGTCTCTAATTTGTCCTCA+TGG | - | chr3.3:73293813-73293832 | None:intergenic | 40.0% |
| ! | TTTTCTTCCACCTTATGAGC+TGG | - | chr3.3:73293903-73293922 | None:intergenic | 40.0% |
| AAGTCAGAAAAGGCTGGATG+AGG | + | chr3.3:73293971-73293990 | MS.gene75434:CDS | 45.0% | |
| ACACATTCAGGTGGAGAAAG+AGG | + | chr3.3:73293863-73293882 | MS.gene75434:CDS | 45.0% | |
| ACATGTTCCAGCTCATAAGG+TGG | + | chr3.3:73293893-73293912 | MS.gene75434:CDS | 45.0% | |
| CAAAGTCACGTGACACATTC+AGG | + | chr3.3:73293851-73293870 | MS.gene75434:CDS | 45.0% | |
| CAGTGAAAGTCAGAAAAGGC+TGG | + | chr3.3:73293965-73293984 | MS.gene75434:CDS | 45.0% | |
| GGAGCAGTGAAAGTCAGAAA+AGG | + | chr3.3:73293961-73293980 | MS.gene75434:CDS | 45.0% | |
| GTCACATGTTCCAGCTCATA+AGG | + | chr3.3:73293890-73293909 | MS.gene75434:CDS | 45.0% | |
| TGCATGCTCTATCACACTCT+TGG | - | chr3.3:73293833-73293852 | None:intergenic | 45.0% | |
| TTCAGGTGGAGAAAGAGGAT+TGG | + | chr3.3:73293868-73293887 | MS.gene75434:CDS | 45.0% | |
| ! | CTTCTTGCAATTCTCTAGGG+AGG | - | chr3.3:73293788-73293807 | None:intergenic | 45.0% |
| !!! | TAATTTTAATTTATACTTTT+TGG | - | chr3.3:73293575-73293594 | None:intergenic | 5.0% |
| AGTCACGTGACACATTCAGG+TGG | + | chr3.3:73293854-73293873 | MS.gene75434:CDS | 50.0% | |
| CACGTAGGCAACAAGGTTGA+AGG | + | chr3.3:73293937-73293956 | MS.gene75434:CDS | 50.0% | |
| CTTCAACCTTGTTGCCTACG+TGG | - | chr3.3:73293939-73293958 | None:intergenic | 50.0% | |
| GTAGGCAACAAGGTTGAAGG+AGG | + | chr3.3:73293940-73293959 | MS.gene75434:CDS | 50.0% | |
| TGTATGCCACGTAGGCAACA+AGG | + | chr3.3:73293930-73293949 | MS.gene75434:CDS | 50.0% | |
| TTTGTGTGGGAACCTACCCT+TGG | + | chr3.3:73293646-73293665 | MS.gene75434:CDS | 50.0% | |
| ! | CTTGCAATTCTCTAGGGAGG+AGG | - | chr3.3:73293785-73293804 | None:intergenic | 50.0% |
| ! | GGAAAAGAGCACGGTCTCAA+AGG | + | chr3.3:73293756-73293775 | MS.gene75434:CDS | 50.0% |
| AAGGAAATGGGGTAGCCTCG+TGG | + | chr3.3:73293734-73293753 | MS.gene75434:CDS | 55.0% | |
| AGCCTCGTGGGAAAAGAGCA+CGG | + | chr3.3:73293747-73293766 | MS.gene75434:CDS | 55.0% | |
| AGGAAATGGGGTAGCCTCGT+GGG | + | chr3.3:73293735-73293754 | MS.gene75434:CDS | 55.0% | |
| ! | ACTGGAGGTGTATGCCACGT+AGG | + | chr3.3:73293922-73293941 | MS.gene75434:CDS | 55.0% |
| ! | GACCGTGCTCTTTTCCCACG+AGG | - | chr3.3:73293752-73293771 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 73293550 | 73294029 | 73293550 | ID=MS.gene75434 |
| chr3.3 | mRNA | 73293550 | 73294029 | 73293550 | ID=MS.gene75434.t1;Parent=MS.gene75434 |
| chr3.3 | exon | 73293550 | 73293578 | 73293550 | ID=MS.gene75434.t1.exon1;Parent=MS.gene75434.t1 |
| chr3.3 | CDS | 73293550 | 73293578 | 73293550 | ID=cds.MS.gene75434.t1;Parent=MS.gene75434.t1 |
| chr3.3 | exon | 73293639 | 73294029 | 73293639 | ID=MS.gene75434.t1.exon2;Parent=MS.gene75434.t1 |
| chr3.3 | CDS | 73293639 | 73294029 | 73293639 | ID=cds.MS.gene75434.t1;Parent=MS.gene75434.t1 |
| Gene Sequence |
| Protein sequence |
