Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75471.t1 | XP_003601959.2 | 78.4 | 199 | 43 | 0 | 1 | 199 | 1 | 199 | 4.20E-87 | 330.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75471.t1 | G7J3S5 | 78.4 | 199 | 43 | 0 | 1 | 199 | 1 | 199 | 3.0e-87 | 330.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene75471 | 0.839238 | 1.79E-57 | -1.69E-46 |
| MS.gene049813 | MS.gene75471 | 0.80949 | 1.84E-50 | -1.69E-46 |
| MS.gene051844 | MS.gene75471 | 0.831021 | 2.11E-55 | -1.69E-46 |
| MS.gene051996 | MS.gene75471 | 0.801958 | 7.04E-49 | -1.69E-46 |
| MS.gene052211 | MS.gene75471 | 0.884947 | 1.24E-71 | -1.69E-46 |
| MS.gene052212 | MS.gene75471 | 0.894071 | 3.48E-75 | -1.69E-46 |
| MS.gene05240 | MS.gene75471 | 0.819349 | 1.22E-52 | -1.69E-46 |
| MS.gene053406 | MS.gene75471 | 0.83833 | 3.07E-57 | -1.69E-46 |
| MS.gene053887 | MS.gene75471 | 0.803042 | 4.21E-49 | -1.69E-46 |
| MS.gene054146 | MS.gene75471 | 0.853352 | 2.53E-61 | -1.69E-46 |
| MS.gene054181 | MS.gene75471 | 0.806586 | 7.66E-50 | -1.69E-46 |
| MS.gene054277 | MS.gene75471 | 0.803478 | 3.42E-49 | -1.69E-46 |
| MS.gene054742 | MS.gene75471 | 0.801247 | 9.85E-49 | -1.69E-46 |
| MS.gene055240 | MS.gene75471 | 0.843732 | 1.17E-58 | -1.69E-46 |
| MS.gene056218 | MS.gene75471 | 0.830653 | 2.60E-55 | -1.69E-46 |
| MS.gene056748 | MS.gene75471 | 0.801649 | 8.15E-49 | -1.69E-46 |
| MS.gene056920 | MS.gene75471 | 0.808318 | 3.29E-50 | -1.69E-46 |
| MS.gene056922 | MS.gene75471 | 0.801072 | 1.07E-48 | -1.69E-46 |
| MS.gene058603 | MS.gene75471 | 0.823845 | 1.11E-53 | -1.69E-46 |
| MS.gene059953 | MS.gene75471 | 0.812952 | 3.27E-51 | -1.69E-46 |
| MS.gene059955 | MS.gene75471 | 0.847948 | 8.37E-60 | -1.69E-46 |
| MS.gene060087 | MS.gene75471 | 0.8073 | 5.41E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75471.t1 | MTR_3g087280 | 86.935 | 199 | 26 | 0 | 1 | 199 | 1 | 199 | 3.70e-132 | 369 |
| MS.gene75471.t1 | MTR_3g087278 | 85.427 | 199 | 29 | 0 | 1 | 199 | 1 | 199 | 2.50e-129 | 362 |
| MS.gene75471.t1 | MTR_3g087325 | 86.935 | 199 | 26 | 0 | 1 | 199 | 1 | 199 | 2.70e-129 | 362 |
| MS.gene75471.t1 | MTR_3g087275 | 86.935 | 199 | 25 | 1 | 1 | 199 | 1 | 198 | 1.76e-127 | 357 |
| MS.gene75471.t1 | MTR_3g087270 | 83.920 | 199 | 32 | 0 | 1 | 199 | 1 | 199 | 1.55e-125 | 352 |
| MS.gene75471.t1 | MTR_3g087330 | 82.412 | 199 | 35 | 0 | 1 | 199 | 1 | 199 | 1.42e-124 | 350 |
| MS.gene75471.t1 | MTR_3g087300 | 68.020 | 197 | 19 | 3 | 1 | 197 | 1 | 153 | 7.59e-88 | 257 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75471.t1 | AT5G65030 | 31.472 | 197 | 110 | 7 | 11 | 187 | 10 | 201 | 8.33e-25 | 96.7 |
| MS.gene75471.t1 | AT5G10210 | 30.808 | 198 | 111 | 8 | 15 | 199 | 13 | 197 | 4.57e-24 | 94.4 |
Find 38 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGCCAAATCATTGCCCTTT+TGG | 0.190841 | 3.3:-72770458 | MS.gene75471:CDS |
| ATATGGGGCTCCAAACTAAT+TGG | 0.223958 | 3.3:-72770342 | MS.gene75471:CDS |
| ACACTAGGCTTTGTTGATTA+AGG | 0.234215 | 3.3:+72770385 | None:intergenic |
| CTCACCTTTCCCAATTAGTT+TGG | 0.258127 | 3.3:+72770332 | None:intergenic |
| TTAATCTCCACTTCCACTTC+TGG | 0.281665 | 3.3:+72770204 | None:intergenic |
| AGCTAAGGCAAAGGAAGATA+TGG | 0.333930 | 3.3:-72770359 | MS.gene75471:CDS |
| TGCACTTGGCGCAGCTTTGG+AGG | 0.390376 | 3.3:-72770052 | MS.gene75471:CDS |
| GTCCCAAAAGGGCAATGATT+TGG | 0.392085 | 3.3:+72770455 | None:intergenic |
| ATTTGCACTTGGCGCAGCTT+TGG | 0.400082 | 3.3:-72770055 | MS.gene75471:CDS |
| GATTCAAGAATCACTTTCCA+TGG | 0.437928 | 3.3:+72770306 | None:intergenic |
| TATGGGGCTCCAAACTAATT+GGG | 0.456932 | 3.3:-72770341 | MS.gene75471:CDS |
| GCTAAGGCAAAGGAAGATAT+GGG | 0.457270 | 3.3:-72770358 | MS.gene75471:CDS |
| AAAATGGTTGAAGATGGATT+TGG | 0.470551 | 3.3:-72770265 | MS.gene75471:CDS |
| AGTAGCTTCGGTTGCTGAGA+TGG | 0.471131 | 3.3:-72770175 | MS.gene75471:CDS |
| GATTAAAATAAAAGTAGCTT+CGG | 0.474275 | 3.3:-72770187 | MS.gene75471:CDS |
| ACTAAATGACTCGTCCCAAA+AGG | 0.477554 | 3.3:+72770443 | None:intergenic |
| CTGTGAATTGAGAATAATAC+AGG | 0.480110 | 3.3:-72770583 | MS.gene75471:CDS |
| TTGGGAAAGGTGAGATTCCA+TGG | 0.490587 | 3.3:-72770323 | MS.gene75471:CDS |
| AAAGCCTAGTGTTGGAGCTA+AGG | 0.499139 | 3.3:-72770374 | MS.gene75471:CDS |
| TTTGCCTTAGCTCCAACACT+AGG | 0.507447 | 3.3:+72770370 | None:intergenic |
| AGATGGAGAAGCAAAATAAG+AGG | 0.509817 | 3.3:-72770158 | MS.gene75471:CDS |
| CTAAATGACTCGTCCCAAAA+GGG | 0.528554 | 3.3:+72770444 | None:intergenic |
| ATGAATAATTTGAATGAGTG+TGG | 0.533229 | 3.3:-72770132 | MS.gene75471:CDS |
| ACGGCCATGACCATCGTGCT+TGG | 0.538014 | 3.3:-72770101 | MS.gene75471:CDS |
| CCAAAAGGGCAATGATTTGG+CGG | 0.551334 | 3.3:+72770458 | None:intergenic |
| TAATCAACAAAGCCTAGTGT+TGG | 0.564525 | 3.3:-72770382 | MS.gene75471:CDS |
| AGCACCAAGCACGATGGTCA+TGG | 0.566327 | 3.3:+72770097 | None:intergenic |
| AGTCGAAGTGATTGCAAAGA+GGG | 0.568276 | 3.3:-72770240 | MS.gene75471:CDS |
| CATAACGCCAGAAGTGGAAG+TGG | 0.570407 | 3.3:-72770211 | MS.gene75471:CDS |
| GATTCTTGAATCACAAAACA+TGG | 0.583125 | 3.3:-72770295 | MS.gene75471:CDS |
| TATGTTCATAACGCCAGAAG+TGG | 0.583659 | 3.3:-72770217 | MS.gene75471:CDS |
| ACATATGAAGATACATGAGA+TGG | 0.585374 | 3.3:+72770615 | None:intergenic |
| TAGTGTTGGAGCTAAGGCAA+AGG | 0.590191 | 3.3:-72770368 | MS.gene75471:CDS |
| GATTGTGATATATTTGCACT+TGG | 0.591421 | 3.3:-72770066 | MS.gene75471:CDS |
| GGCTCCAAACTAATTGGGAA+AGG | 0.604038 | 3.3:-72770336 | MS.gene75471:CDS |
| GAGTCGAAGTGATTGCAAAG+AGG | 0.661989 | 3.3:-72770241 | MS.gene75471:CDS |
| CAGCGCAGCACCAAGCACGA+TGG | 0.681591 | 3.3:+72770091 | None:intergenic |
| CTAAGGCAAAGGAAGATATG+GGG | 0.687073 | 3.3:-72770357 | MS.gene75471:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTAAAATAAAAGTAGCTT+CGG | - | chr3.3:72770478-72770497 | MS.gene75471:CDS | 20.0% |
| ! | AAAACATGGAGCTAAAAAAA+TGG | - | chr3.3:72770384-72770403 | MS.gene75471:CDS | 25.0% |
| ! | ATGAATAATTTGAATGAGTG+TGG | - | chr3.3:72770533-72770552 | MS.gene75471:CDS | 25.0% |
| ACATATGAAGATACATGAGA+TGG | + | chr3.3:72770053-72770072 | None:intergenic | 30.0% | |
| CTGTGAATTGAGAATAATAC+AGG | - | chr3.3:72770082-72770101 | MS.gene75471:CDS | 30.0% | |
| GATTCTTGAATCACAAAACA+TGG | - | chr3.3:72770370-72770389 | MS.gene75471:CDS | 30.0% | |
| GATTGTGATATATTTGCACT+TGG | - | chr3.3:72770599-72770618 | MS.gene75471:CDS | 30.0% | |
| GCTAAAAAAATGGTTGAAGA+TGG | - | chr3.3:72770394-72770413 | MS.gene75471:CDS | 30.0% | |
| ! | AAAATGGTTGAAGATGGATT+TGG | - | chr3.3:72770400-72770419 | MS.gene75471:CDS | 30.0% |
| ! | AAGGTTTTCCATGAAATCTT+GGG | + | chr3.3:72770264-72770283 | None:intergenic | 30.0% |
| ! | TAAGGTTTTCCATGAAATCT+TGG | + | chr3.3:72770265-72770284 | None:intergenic | 30.0% |
| !!! | ATTCTTTTGTTGTTCTCTGT+TGG | + | chr3.3:72770164-72770183 | None:intergenic | 30.0% |
| AATGAGTGTGGATGCAAAAA+CGG | - | chr3.3:72770545-72770564 | MS.gene75471:CDS | 35.0% | |
| AGATGGAGAAGCAAAATAAG+AGG | - | chr3.3:72770507-72770526 | MS.gene75471:CDS | 35.0% | |
| GATTCAAGAATCACTTTCCA+TGG | + | chr3.3:72770362-72770381 | None:intergenic | 35.0% | |
| TAATCAACAAAGCCTAGTGT+TGG | - | chr3.3:72770283-72770302 | MS.gene75471:CDS | 35.0% | |
| ! | AGGTTTTCCATGAAATCTTG+GGG | + | chr3.3:72770263-72770282 | None:intergenic | 35.0% |
| !! | ACACTAGGCTTTGTTGATTA+AGG | + | chr3.3:72770283-72770302 | None:intergenic | 35.0% |
| ACTAAATGACTCGTCCCAAA+AGG | + | chr3.3:72770225-72770244 | None:intergenic | 40.0% | |
| AGCTAAGGCAAAGGAAGATA+TGG | - | chr3.3:72770306-72770325 | MS.gene75471:CDS | 40.0% | |
| AGTCGAAGTGATTGCAAAGA+GGG | - | chr3.3:72770425-72770444 | MS.gene75471:CDS | 40.0% | |
| ATATGGGGCTCCAAACTAAT+TGG | - | chr3.3:72770323-72770342 | MS.gene75471:CDS | 40.0% | |
| CTAAATGACTCGTCCCAAAA+GGG | + | chr3.3:72770224-72770243 | None:intergenic | 40.0% | |
| CTAAGGCAAAGGAAGATATG+GGG | - | chr3.3:72770308-72770327 | MS.gene75471:CDS | 40.0% | |
| CTCACCTTTCCCAATTAGTT+TGG | + | chr3.3:72770336-72770355 | None:intergenic | 40.0% | |
| GCTAAGGCAAAGGAAGATAT+GGG | - | chr3.3:72770307-72770326 | MS.gene75471:CDS | 40.0% | |
| TATGGGGCTCCAAACTAATT+GGG | - | chr3.3:72770324-72770343 | MS.gene75471:CDS | 40.0% | |
| TATGTTCATAACGCCAGAAG+TGG | - | chr3.3:72770448-72770467 | MS.gene75471:CDS | 40.0% | |
| TTAATCTCCACTTCCACTTC+TGG | + | chr3.3:72770464-72770483 | None:intergenic | 40.0% | |
| ! | CGACGAAAAACTTGTTTGCA+AGG | - | chr3.3:72770129-72770148 | MS.gene75471:CDS | 40.0% |
| ! | GGTTTTCCATGAAATCTTGG+GGG | + | chr3.3:72770262-72770281 | None:intergenic | 40.0% |
| GAGTCGAAGTGATTGCAAAG+AGG | - | chr3.3:72770424-72770443 | MS.gene75471:CDS | 45.0% | |
| GGCTCCAAACTAATTGGGAA+AGG | - | chr3.3:72770329-72770348 | MS.gene75471:CDS | 45.0% | |
| GTCCCAAAAGGGCAATGATT+TGG | + | chr3.3:72770213-72770232 | None:intergenic | 45.0% | |
| TTCTTGCCCCCAAGATTTCA+TGG | - | chr3.3:72770253-72770272 | MS.gene75471:CDS | 45.0% | |
| TTGGGAAAGGTGAGATTCCA+TGG | - | chr3.3:72770342-72770361 | MS.gene75471:CDS | 45.0% | |
| TTTGCCTTAGCTCCAACACT+AGG | + | chr3.3:72770298-72770317 | None:intergenic | 45.0% | |
| ! | AAAGCCTAGTGTTGGAGCTA+AGG | - | chr3.3:72770291-72770310 | MS.gene75471:CDS | 45.0% |
| ! | CCAAAAGGGCAATGATTTGG+CGG | + | chr3.3:72770210-72770229 | None:intergenic | 45.0% |
| ! | CGCCAAATCATTGCCCTTTT+GGG | - | chr3.3:72770208-72770227 | MS.gene75471:CDS | 45.0% |
| ! | TAGTGTTGGAGCTAAGGCAA+AGG | - | chr3.3:72770297-72770316 | MS.gene75471:CDS | 45.0% |
| AGTAGCTTCGGTTGCTGAGA+TGG | - | chr3.3:72770490-72770509 | MS.gene75471:CDS | 50.0% | |
| ATTTGCACTTGGCGCAGCTT+TGG | - | chr3.3:72770610-72770629 | MS.gene75471:CDS | 50.0% | |
| CATAACGCCAGAAGTGGAAG+TGG | - | chr3.3:72770454-72770473 | MS.gene75471:CDS | 50.0% | |
| ! | CCGCCAAATCATTGCCCTTT+TGG | - | chr3.3:72770207-72770226 | MS.gene75471:CDS | 50.0% |
| AGCACCAAGCACGATGGTCA+TGG | + | chr3.3:72770571-72770590 | None:intergenic | 55.0% | |
| ACGGCCATGACCATCGTGCT+TGG | - | chr3.3:72770564-72770583 | MS.gene75471:CDS | 60.0% | |
| ! | TGCACTTGGCGCAGCTTTGG+AGG | - | chr3.3:72770613-72770632 | MS.gene75471:CDS | 60.0% |
| CAGCGCAGCACCAAGCACGA+TGG | + | chr3.3:72770577-72770596 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 72770044 | 72770643 | 72770044 | ID=MS.gene75471 |
| chr3.3 | mRNA | 72770044 | 72770643 | 72770044 | ID=MS.gene75471.t1;Parent=MS.gene75471 |
| chr3.3 | exon | 72770044 | 72770643 | 72770044 | ID=MS.gene75471.t1.exon1;Parent=MS.gene75471.t1 |
| chr3.3 | CDS | 72770044 | 72770643 | 72770044 | ID=cds.MS.gene75471.t1;Parent=MS.gene75471.t1 |
| Gene Sequence |
| Protein sequence |
