AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76296

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76296.t1 MTR_1g111010 96.857 350 11 0 13 362 1 350 0.0 714
MS.gene76296.t1 MTR_2g073500 64.000 350 114 5 13 354 1 346 1.55e-162 459
MS.gene76296.t1 MTR_2g073510 47.207 358 165 9 15 353 1 353 4.51e-110 325
MS.gene76296.t1 MTR_3g049330 48.138 349 158 10 22 354 10 351 5.67e-107 318
MS.gene76296.t1 MTR_2g015720 48.780 328 149 10 39 353 37 358 2.58e-100 301
MS.gene76296.t1 MTR_4g088030 45.584 351 170 11 17 354 9 351 3.15e-99 298
MS.gene76296.t1 MTR_4g087730 46.429 336 162 10 32 354 33 363 8.18e-97 292
MS.gene76296.t1 MTR_3g021060 45.045 333 163 9 35 353 37 363 5.26e-95 288
MS.gene76296.t1 MTR_5g034810 45.783 332 160 10 39 356 30 355 9.03e-94 284
MS.gene76296.t1 MTR_2g091175 46.526 331 152 11 41 355 47 368 1.76e-93 284
MS.gene76296.t1 MTR_3g035830 44.510 337 167 9 31 353 18 348 2.60e-93 283
MS.gene76296.t1 MTR_3g021090 45.210 334 161 10 31 349 18 344 4.45e-92 281
MS.gene76296.t1 MTR_4g079700 45.732 328 155 10 41 353 44 363 1.95e-91 278
MS.gene76296.t1 MTR_3g464570 41.329 346 169 11 39 354 36 377 6.12e-85 262
MS.gene76296.t1 MTR_5g071350 41.341 358 169 11 34 354 47 400 2.11e-83 259
MS.gene76296.t1 MTR_4g121560 39.884 346 170 11 43 354 113 454 1.97e-78 248
MS.gene76296.t1 MTR_1g050448 39.759 332 175 10 41 353 50 375 1.16e-77 243
MS.gene76296.t1 MTR_7g093450 38.682 349 178 12 43 358 247 592 1.60e-77 249
MS.gene76296.t1 MTR_1g112210 40.361 332 172 12 42 353 57 382 5.77e-77 242
MS.gene76296.t1 MTR_0136s0010 39.420 345 173 12 43 354 169 510 6.26e-77 246
MS.gene76296.t1 MTR_1g112210 39.759 332 161 12 42 353 57 369 3.32e-75 237
MS.gene76296.t1 MTR_7g109550 39.759 332 171 11 44 350 137 464 4.97e-75 239
MS.gene76296.t1 MTR_4g016940 36.127 346 185 10 42 353 84 427 1.32e-70 227
MS.gene76296.t1 MTR_5g033800 36.311 347 182 11 42 354 143 484 2.41e-70 228
MS.gene76296.t1 MTR_8g069790 36.416 346 181 11 43 354 177 517 1.60e-69 227
MS.gene76296.t1 MTR_4g126950 36.888 347 185 12 42 357 107 450 4.69e-69 224
MS.gene76296.t1 MTR_5g059400 38.261 345 176 11 44 354 175 516 1.78e-68 224
MS.gene76296.t1 MTR_1g116130 38.261 345 176 11 44 354 175 516 1.78e-68 224
MS.gene76296.t1 MTR_8g463790 33.333 345 195 9 44 356 71 412 8.62e-67 217
MS.gene76296.t1 MTR_3g090120 36.364 341 188 12 39 354 124 460 2.88e-66 217
MS.gene76296.t1 MTR_7g094750 37.006 354 185 13 41 359 88 438 5.35e-65 213
MS.gene76296.t1 MTR_3g070900 35.429 350 188 10 42 355 104 451 1.12e-64 212
MS.gene76296.t1 MTR_4g014430 34.682 346 193 8 39 353 103 446 2.95e-64 211
MS.gene76296.t1 MTR_7g068920 34.670 349 188 10 41 353 77 421 3.21e-64 210
MS.gene76296.t1 MTR_2g022560 38.150 346 175 12 44 354 97 438 1.33e-61 204
MS.gene76296.t1 MTR_3g071650 35.143 350 181 11 44 354 89 431 7.98e-61 201
MS.gene76296.t1 MTR_1g058380 34.043 376 205 14 17 358 33 399 1.17e-60 201
MS.gene76296.t1 MTR_8g093530 36.391 338 182 13 42 354 88 417 2.06e-60 201
MS.gene76296.t1 MTR_4g014440 31.868 364 206 10 30 354 48 408 2.57e-60 199
MS.gene76296.t1 MTR_5g082880 33.994 353 192 11 42 355 163 513 3.06e-58 197
MS.gene76296.t1 MTR_3g464570 38.971 272 136 8 111 354 4 273 1.08e-57 189
MS.gene76296.t1 MTR_5g083260 33.333 345 194 10 44 354 83 425 2.50e-56 190
MS.gene76296.t1 MTR_7g070060 30.899 356 204 10 39 355 59 411 1.45e-55 187
MS.gene76296.t1 MTR_5g033800 36.515 241 132 6 42 266 143 378 8.93e-51 174
MS.gene76296.t1 MTR_7g094750 35.537 242 137 7 41 266 88 326 9.30e-44 154
MS.gene76296.t1 MTR_7g070060 31.535 241 154 5 41 271 61 300 1.86e-39 142
MS.gene76296.t1 MTR_1g076690 31.044 364 181 15 44 353 223 570 5.12e-37 140
MS.gene76296.t1 MTR_7g070060 28.065 310 181 10 86 355 2 309 3.90e-36 133
MS.gene76296.t1 MTR_6g061710 27.793 367 189 14 44 353 167 514 3.69e-34 132
MS.gene76296.t1 MTR_4g092830 28.011 357 200 15 44 354 48 393 8.89e-33 127
MS.gene76296.t1 MTR_6g016875 27.528 356 211 12 39 353 59 408 1.54e-31 123
MS.gene76296.t1 MTR_5g035090 28.409 352 211 12 40 353 79 427 2.13e-31 123
MS.gene76296.t1 MTR_2g016060 29.444 360 189 18 45 353 56 401 9.17e-31 121
MS.gene76296.t1 MTR_6g016880 27.966 354 203 13 42 353 76 419 1.16e-30 121
MS.gene76296.t1 MTR_8g093870 28.490 351 203 13 42 353 131 472 4.94e-30 120
MS.gene76296.t1 MTR_5g035150 28.142 366 212 15 32 353 73 431 5.06e-28 114
MS.gene76296.t1 MTR_6g007777 27.374 358 218 13 33 353 105 457 6.18e-28 114
MS.gene76296.t1 MTR_5g069320 32.090 268 123 10 101 346 156 386 9.49e-28 112
MS.gene76296.t1 MTR_7g019120 27.733 375 215 15 23 353 58 420 6.07e-27 111
MS.gene76296.t1 MTR_6g016240 25.648 347 214 11 43 349 70 412 8.23e-27 110
MS.gene76296.t1 MTR_3g109840 27.778 360 209 15 44 361 73 423 1.19e-26 110
MS.gene76296.t1 MTR_8g027900 27.321 377 217 17 25 354 190 556 2.73e-26 110
MS.gene76296.t1 MTR_7g033230 28.333 360 210 12 42 359 262 615 5.60e-25 106
MS.gene76296.t1 MTR_7g033230 28.333 360 210 12 42 359 332 685 1.36e-24 105
MS.gene76296.t1 MTR_4g125060 27.066 351 208 12 42 353 130 471 6.32e-24 102
MS.gene76296.t1 MTR_5g035070 27.401 354 206 15 44 353 87 433 1.05e-23 102
MS.gene76296.t1 MTR_4g125060 27.066 351 208 12 42 353 169 510 1.30e-23 102
MS.gene76296.t1 MTR_7g019140 42.478 113 63 2 23 135 58 168 1.66e-23 95.9
MS.gene76296.t1 MTR_4g125060 27.066 351 208 12 42 353 198 539 1.78e-23 102
MS.gene76296.t1 MTR_8g104970 27.654 358 207 13 40 353 116 465 1.96e-23 101
MS.gene76296.t1 MTR_8g099300 25.281 356 216 14 43 355 73 421 4.04e-22 97.1
MS.gene76296.t1 MTR_5g035080 42.727 110 55 3 31 134 56 163 2.10e-20 92.4
MS.gene76296.t1 MTR_3g021150 40.952 105 51 2 229 322 1 105 2.04e-19 83.2
MS.gene76296.t1 MTR_5g035120 44.211 95 51 2 40 134 71 163 2.63e-19 84.7
MS.gene76296.t1 MTR_2g022580 34.188 117 64 5 106 209 2 118 3.09e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76296.t1 AT1G78710 51.205 332 141 9 38 354 32 357 4.30e-116 341
MS.gene76296.t1 AT2G30900 47.706 327 152 8 44 354 43 366 9.69e-103 307
MS.gene76296.t1 AT1G29050 46.341 328 156 11 40 353 57 378 7.84e-96 290
MS.gene76296.t1 AT2G34070 46.769 325 152 10 44 353 65 383 1.29e-95 290
MS.gene76296.t1 AT3G14850 45.921 331 156 10 41 354 31 355 1.74e-94 286
MS.gene76296.t1 AT2G42570 46.246 333 157 10 39 356 42 367 9.42e-94 285
MS.gene76296.t1 AT2G31110 45.399 326 156 11 43 353 44 362 7.50e-91 277
MS.gene76296.t1 AT1G78710 44.582 323 123 9 38 354 25 297 9.43e-89 269
MS.gene76296.t1 AT3G54260 43.544 333 162 11 44 356 51 377 2.43e-82 256
MS.gene76296.t1 AT5G58600 42.105 342 161 12 44 354 66 401 1.17e-80 252
MS.gene76296.t1 AT2G30010 41.737 357 163 13 36 354 48 397 2.47e-80 251
MS.gene76296.t1 AT2G30010 41.737 357 163 13 36 354 91 440 8.34e-80 251
MS.gene76296.t1 AT3G62390 41.471 340 169 12 42 354 136 472 4.75e-79 250
MS.gene76296.t1 AT5G49340 38.506 348 172 10 44 354 100 442 1.88e-78 248
MS.gene76296.t1 AT2G30900 52.252 222 99 6 44 261 43 261 2.72e-74 231
MS.gene76296.t1 AT3G12060 38.150 346 178 11 42 354 194 536 2.53e-72 235
MS.gene76296.t1 AT5G20590 39.003 341 177 14 44 354 141 480 1.58e-71 231
MS.gene76296.t1 AT5G06700 36.994 346 182 13 42 354 253 595 9.23e-71 232
MS.gene76296.t1 AT5G01360 35.159 347 188 9 42 353 34 378 2.19e-70 225
MS.gene76296.t1 AT5G01360 35.159 347 188 9 42 353 90 434 1.11e-69 224
MS.gene76296.t1 AT1G60790 36.752 351 179 11 42 354 188 533 2.55e-69 227
MS.gene76296.t1 AT2G31110 47.845 232 111 8 43 271 44 268 3.04e-68 216
MS.gene76296.t1 AT1G73140 33.623 345 194 9 42 354 65 406 2.89e-67 218
MS.gene76296.t1 AT1G48880 37.685 337 183 11 39 354 110 440 2.20e-66 216
MS.gene76296.t1 AT2G34070 44.664 253 121 8 116 353 1 249 8.95e-65 206
MS.gene76296.t1 AT3G55990 35.143 350 189 9 42 355 138 485 1.13e-63 211
MS.gene76296.t1 AT5G06230 35.754 358 184 12 41 358 18 369 3.81e-62 203
MS.gene76296.t1 AT5G19160 37.535 357 177 16 44 359 99 450 3.90e-62 206
MS.gene76296.t1 AT3G14850 44.048 252 120 8 116 350 1 248 4.02e-62 199
MS.gene76296.t1 AT5G06230 35.754 358 184 12 41 358 59 410 8.13e-62 204
MS.gene76296.t1 AT3G06080 37.536 349 181 14 44 357 103 449 6.05e-61 203
MS.gene76296.t1 AT2G40160 34.819 359 175 12 44 354 77 424 2.22e-60 201
MS.gene76296.t1 AT2G40160 34.746 354 172 12 44 349 77 419 9.56e-60 199
MS.gene76296.t1 AT5G01620 32.394 355 200 11 34 353 100 449 1.38e-59 199
MS.gene76296.t1 AT5G01620 32.394 355 200 11 34 353 100 449 1.38e-59 199
MS.gene76296.t1 AT5G01620 32.394 355 200 11 34 353 108 457 2.08e-59 199
MS.gene76296.t1 AT2G40320 33.524 349 192 11 42 354 79 423 2.77e-57 192
MS.gene76296.t1 AT3G11570 36.420 324 174 11 66 358 102 424 3.58e-57 192
MS.gene76296.t1 AT2G40150 32.773 357 196 11 43 357 70 424 4.26e-57 192
MS.gene76296.t1 AT2G38320 32.955 352 196 12 41 354 56 405 6.47e-56 188
MS.gene76296.t1 AT3G11030 33.621 348 194 11 42 354 104 449 1.53e-55 188
MS.gene76296.t1 AT5G58600 44.298 228 118 8 44 266 66 289 1.71e-55 184
MS.gene76296.t1 AT3G62390 41.057 246 128 7 42 273 136 378 2.39e-55 187
MS.gene76296.t1 AT5G20590 39.241 237 129 8 44 266 141 376 1.40e-51 176
MS.gene76296.t1 AT2G38320 32.177 317 176 11 76 354 7 322 3.77e-46 160
MS.gene76296.t1 AT2G40160 36.475 244 124 6 44 266 77 310 2.20e-45 158
MS.gene76296.t1 AT5G19160 39.076 238 128 8 44 266 99 334 3.05e-45 159
MS.gene76296.t1 AT2G31110 41.905 210 103 7 159 353 9 214 3.38e-44 152
MS.gene76296.t1 AT3G06080 39.496 238 127 9 44 266 103 338 1.66e-43 154
MS.gene76296.t1 AT5G06230 35.060 251 132 8 41 266 59 303 1.05e-40 145
MS.gene76296.t1 AT5G64470 28.857 350 205 12 44 356 55 397 1.20e-35 134
MS.gene76296.t1 AT3G28150 30.286 350 197 11 43 353 69 410 2.08e-35 134
MS.gene76296.t1 AT5G64470 28.652 356 204 13 44 356 55 403 4.94e-35 132
MS.gene76296.t1 AT4G25360 29.640 361 194 14 43 353 174 524 1.10e-34 134
MS.gene76296.t1 AT4G25360 29.640 361 194 14 43 353 174 524 1.10e-34 134
MS.gene76296.t1 AT5G51640 28.809 361 198 13 43 353 137 488 1.19e-34 133
MS.gene76296.t1 AT2G14530 28.652 356 203 11 41 353 59 406 2.12e-34 131
MS.gene76296.t1 AT1G70230 29.155 343 200 16 44 353 79 411 8.53e-32 124
MS.gene76296.t1 AT1G01430 28.689 366 210 14 41 361 96 455 6.23e-31 122
MS.gene76296.t1 AT4G01080 28.011 357 211 14 39 357 86 434 5.71e-29 117
MS.gene76296.t1 AT3G02440 28.531 354 197 15 44 353 127 468 8.37e-28 114
MS.gene76296.t1 AT3G02440 28.531 354 197 15 44 353 135 476 9.96e-28 114
MS.gene76296.t1 AT2G37720 27.171 357 216 13 34 353 133 482 3.59e-27 112
MS.gene76296.t1 AT5G15900 28.771 358 192 16 44 353 86 428 1.81e-26 109
MS.gene76296.t1 AT5G15900 28.771 358 192 16 44 353 67 409 1.86e-26 109
MS.gene76296.t1 AT5G20680 26.404 356 204 14 42 353 116 457 1.40e-25 107
MS.gene76296.t1 AT5G20680 26.816 358 200 15 42 353 210 551 1.55e-25 108
MS.gene76296.t1 AT5G20680 26.816 358 200 15 42 353 210 551 1.55e-25 108
MS.gene76296.t1 AT5G20680 26.816 358 200 15 42 353 210 551 1.55e-25 108
MS.gene76296.t1 AT5G20680 26.816 358 200 15 42 353 210 551 1.55e-25 108
MS.gene76296.t1 AT5G20680 26.816 358 200 15 42 353 192 533 1.64e-25 107
MS.gene76296.t1 AT5G64020 27.528 356 208 13 44 353 57 408 4.50e-25 105
MS.gene76296.t1 AT5G15890 27.841 352 200 13 42 353 185 522 5.07e-25 106
MS.gene76296.t1 AT4G11090 24.722 360 226 11 39 357 74 429 1.08e-23 102
MS.gene76296.t1 AT4G23790 26.593 361 216 12 39 355 74 429 5.92e-23 99.8
MS.gene76296.t1 AT5G64470 28.395 243 149 9 44 266 55 292 9.99e-21 92.0
MS.gene76296.t1 AT5G64470 28.395 243 149 9 44 266 55 292 1.13e-20 92.0

Find 76 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTCTATGTTCAGATTTC+TGG 0.137068 1.1:+78358747 MS.gene76296:CDS
CACACACATTCGTTCTTCTA+TGG 0.191029 1.1:+78358055 MS.gene76296:CDS
GGTTAACCACTTGGTCTAAT+TGG 0.192861 1.1:+78359401 MS.gene76296:CDS
ACATGAAAAGTCATGTTTAT+TGG 0.227466 1.1:+78360083 MS.gene76296:CDS
TCAAATCCCTGCACTATAAA+AGG 0.249740 1.1:-78358189 None:intergenic
GGAATTGCTGCTGCACATTC+AGG 0.255467 1.1:+78359469 MS.gene76296:CDS
GTAAAGGTGTAATTTGATTC+TGG 0.257863 1.1:-78358709 None:intergenic
CAAACAATCATGGTTTGGTT+TGG 0.329748 1.1:+78357979 None:intergenic
AACATTGAAGCTTACAAGAT+TGG 0.344348 1.1:+78359379 MS.gene76296:CDS
AAGACTACTGTTATCTTTCA+AGG 0.351212 1.1:+78359448 MS.gene76296:CDS
CCTCGACCAGTGAATATAGA+AGG 0.368131 1.1:-78360148 None:intergenic
GTTTGATGGTGTGAAATTCT+TGG 0.369119 1.1:+78358594 MS.gene76296:CDS
GCATCATAAAGAGGGTAGTA+TGG 0.369410 1.1:-78358153 None:intergenic
AAGATTATCTCAAATATAGA+TGG 0.394741 1.1:+78358238 MS.gene76296:CDS
GCTGGCTTTGATCCTTCCTC+TGG 0.400656 1.1:-78360022 None:intergenic
AATGGCAAACAATCATGGTT+TGG 0.409245 1.1:+78357974 None:intergenic
TGGTTAATTAATAGGTTTGA+TGG 0.412253 1.1:+78358580 MS.gene76296:intron
ACATTGAAGCTTACAAGATT+GGG 0.421501 1.1:+78359380 MS.gene76296:CDS
AAAGATAACAGTAGTCTTTG+AGG 0.421554 1.1:-78359443 None:intergenic
TGCAGTCACTGGTGTCTAGC+TGG 0.427585 1.1:+78360187 MS.gene76296:CDS
CAAACACAACTGCGGATAGA+TGG 0.442377 1.1:+78360121 MS.gene76296:CDS
GTGATCTATCTAAAGGAGAA+TGG 0.447934 1.1:+78358118 MS.gene76296:CDS
GGATTTGATTGTCTCAGAAA+TGG 0.453425 1.1:+78358204 MS.gene76296:CDS
AGCATTTCATTCCAAGTGTC+GGG 0.455839 1.1:-78360214 None:intergenic
TCAATAGTGTGGGTGAAAGA+TGG 0.461620 1.1:+78358884 MS.gene76296:CDS
TAGCTGGTGCTCCCGACACT+TGG 0.461664 1.1:+78360203 MS.gene76296:CDS
TATTTGAGATAATCTTGATC+TGG 0.461942 1.1:-78358231 None:intergenic
TACGGTTCGTGATAGAGAAA+AGG 0.466498 1.1:+78358919 MS.gene76296:CDS
CTTGGAAGATCACATGCAAA+AGG 0.466690 1.1:-78358264 None:intergenic
TGATCTATCTAAAGGAGAAT+GGG 0.480088 1.1:+78358119 MS.gene76296:CDS
AGTATGAAGTTTCAATAGTG+TGG 0.498044 1.1:+78358873 MS.gene76296:CDS
GAATTGCTGCTGCACATTCA+GGG 0.500253 1.1:+78359470 MS.gene76296:CDS
ACACACATTCGTTCTTCTAT+GGG 0.503042 1.1:+78358056 MS.gene76296:CDS
CAAGAATGTGATCTATCTAA+AGG 0.504816 1.1:+78358111 MS.gene76296:CDS
GGATCAAAGCCAGCATATCC+TGG 0.505064 1.1:+78360031 MS.gene76296:CDS
ACAAGATTGGGTTAACCACT+TGG 0.507566 1.1:+78359392 MS.gene76296:CDS
CAATAGTGTGGGTGAAAGAT+GGG 0.508616 1.1:+78358885 MS.gene76296:CDS
AAGATAACAGTAGTCTTTGA+GGG 0.513061 1.1:-78359442 None:intergenic
CAGCATTTCATTCCAAGTGT+CGG 0.523240 1.1:-78360215 None:intergenic
AAGACAATATCAAATGACAT+TGG 0.532659 1.1:+78358011 MS.gene76296:CDS
ACTCTATAAGCGACAATATG+TGG 0.533074 1.1:+78358653 MS.gene76296:CDS
CCACTTGTACCGAGATGAAA+CGG 0.534358 1.1:-78358963 None:intergenic
TCCCTTGAGGCATCATAAAG+AGG 0.534577 1.1:-78358162 None:intergenic
ATGTCTACACCAGGATATGC+TGG 0.536979 1.1:-78360040 None:intergenic
TGGTCGAGGAACTTCTTATG+AGG 0.538598 1.1:+78360162 MS.gene76296:CDS
ACGGTTCGTGATAGAGAAAA+GGG 0.539431 1.1:+78358920 MS.gene76296:CDS
AACTTGACTCCGTTTCATCT+CGG 0.548645 1.1:+78358954 MS.gene76296:CDS
CCCTCTTTATGATGCCTCAA+GGG 0.550562 1.1:+78358161 MS.gene76296:CDS
TGTGTGAAACCACCAATGAT+AGG 0.556830 1.1:-78359011 None:intergenic
CTTATGAGGACTGCAGTCAC+TGG 0.559215 1.1:+78360176 MS.gene76296:CDS
ATCAATCCAATTAGACCAAG+TGG 0.559355 1.1:-78359407 None:intergenic
AGATGGGTACCTAGTGGATA+CGG 0.563975 1.1:+78358901 MS.gene76296:CDS
GGTCACCCTTCTATATTCAC+TGG 0.564907 1.1:+78360142 MS.gene76296:CDS
CCGTTTCATCTCGGTACAAG+TGG 0.575787 1.1:+78358963 MS.gene76296:CDS
ACCCTCTTTATGATGCCTCA+AGG 0.586390 1.1:+78358160 MS.gene76296:CDS
CCTTCTATATTCACTGGTCG+AGG 0.587820 1.1:+78360148 MS.gene76296:CDS
TCATCTCGGTACAAGTGGAA+TGG 0.589411 1.1:+78358968 MS.gene76296:CDS
GTATGAAGTTTCAATAGTGT+GGG 0.591688 1.1:+78358874 MS.gene76296:CDS
ATTCGTTCTTCTATGGGCAG+TGG 0.593313 1.1:+78358062 MS.gene76296:CDS
AAATGAGATAATGAAAGACA+TGG 0.606612 1.1:+78359354 MS.gene76296:CDS
CCCTTGAGGCATCATAAAGA+GGG 0.607432 1.1:-78358161 None:intergenic
GAACAAACTAAACCAGAGGA+AGG 0.620219 1.1:+78360010 MS.gene76296:CDS
CTCGACCAGTGAATATAGAA+GGG 0.620624 1.1:-78360147 None:intergenic
TTCTGAACAATGTCTACACC+AGG 0.627751 1.1:-78360049 None:intergenic
GCATGCACATAGTACAGTGT+AGG 0.634677 1.1:-78358087 None:intergenic
TATCACGAACCGTATCCACT+AGG 0.636942 1.1:-78358910 None:intergenic
CATCCACAGACACTTTCAAG+TGG 0.640996 1.1:+78359330 MS.gene76296:intron
TATCATTGGTGGTTTCACAC+AGG 0.651059 1.1:+78359013 MS.gene76296:CDS
ATCCACAGACACTTTCAAGT+GGG 0.658544 1.1:+78359331 MS.gene76296:intron
TCTGGAACAAACTAAACCAG+AGG 0.676454 1.1:+78360006 MS.gene76296:CDS
TTACTTTACAAACACAACTG+CGG 0.678864 1.1:+78360113 MS.gene76296:CDS
TATAAAAGGACAGTCCCTTG+AGG 0.699934 1.1:-78358175 None:intergenic
AAATTCTTGGAGACATACCG+AGG 0.705651 1.1:+78358607 MS.gene76296:CDS
CATAGAAGAACGAATGTGTG+TGG 0.724411 1.1:-78358054 None:intergenic
AATTCTTGGAGACATACCGA+GGG 0.724500 1.1:+78358608 MS.gene76296:CDS
GGTGAAAGATGGGTACCTAG+TGG 0.748513 1.1:+78358895 MS.gene76296:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTCATTTTAAAAAAAAAA+TGG - chr1.1:78359620-78359639 None:intergenic 10.0%
!! AAAAAAATGGTATATTTACA+AGG - chr1.1:78359607-78359626 None:intergenic 15.0%
!!! AAATTTGTTCATATTGAAAT+TGG - chr1.1:78358502-78358521 None:intergenic 15.0%
!!! AGTTAGTTTTTTTTTCTTAT+TGG + chr1.1:78359172-78359191 MS.gene76296:intron 15.0%
!!! ATTTTCCTTTAATTTAAGAT+TGG - chr1.1:78358788-78358807 None:intergenic 15.0%
!!! CAAACATTTTACATATTATA+AGG - chr1.1:78358437-78358456 None:intergenic 15.0%
!!! GATGCAATATTTATTTTTAT+TGG + chr1.1:78358404-78358423 MS.gene76296:intron 15.0%
!!! GTGAATTTTATAGTTAAAAT+GGG - chr1.1:78359942-78359961 None:intergenic 15.0%
!!! TGTGAATTTTATAGTTAAAA+TGG - chr1.1:78359943-78359962 None:intergenic 15.0%
!!! TTATTTTAGTCTTAATTTAG+TGG - chr1.1:78358469-78358488 None:intergenic 15.0%
!! AAGATTATCTCAAATATAGA+TGG + chr1.1:78358238-78358257 MS.gene76296:CDS 20.0%
!! AATTGTTTGATAAGTTATCT+TGG + chr1.1:78358820-78358839 MS.gene76296:intron 20.0%
!! ATGAAACATTATTATTACCT+TGG - chr1.1:78358285-78358304 None:intergenic 20.0%
!! TAACTTATCAAACAATTATC+AGG - chr1.1:78358817-78358836 None:intergenic 20.0%
!! TCAACTAATACAAAATAACA+AGG - chr1.1:78358386-78358405 None:intergenic 20.0%
!!! AATGCTTCATAAATTTTCAA+GGG + chr1.1:78359973-78359992 MS.gene76296:intron 20.0%
!!! AATTTTAATCCTAACTTTGA+TGG + chr1.1:78359717-78359736 MS.gene76296:intron 20.0%
!!! TAATGCTTCATAAATTTTCA+AGG + chr1.1:78359972-78359991 MS.gene76296:intron 20.0%
!!! TATACAATTAGTTTTGTCTT+AGG - chr1.1:78358546-78358565 None:intergenic 20.0%
!!! TATTTAAAATTAGGAAAGAG+TGG - chr1.1:78358320-78358339 None:intergenic 20.0%
!!! TTTTAGTCTTTGCAAATATA+CGG - chr1.1:78359830-78359849 None:intergenic 20.0%
! AAATGAGATAATGAAAGACA+TGG + chr1.1:78359354-78359373 MS.gene76296:CDS 25.0%
! AAGACAATATCAAATGACAT+TGG + chr1.1:78358011-78358030 MS.gene76296:CDS 25.0%
! ACATGAAAAGTCATGTTTAT+TGG + chr1.1:78360083-78360102 MS.gene76296:CDS 25.0%
! CAACTCCAATCTTAAATTAA+AGG + chr1.1:78358780-78358799 MS.gene76296:intron 25.0%
! GAGAGAGTTATTAGAATATA+AGG + chr1.1:78359138-78359157 MS.gene76296:intron 25.0%
! GCTAAAAAACTTTAGTACAT+TGG - chr1.1:78359046-78359065 None:intergenic 25.0%
! TGGTTAATTAATAGGTTTGA+TGG + chr1.1:78358580-78358599 MS.gene76296:intron 25.0%
!! AGTGATGTTGGTTAATTAAT+AGG + chr1.1:78358572-78358591 MS.gene76296:intron 25.0%
!! AGTTTGTTCTTCTTATCATT+CGG - chr1.1:78359215-78359234 None:intergenic 25.0%
!! ATAAGAACAAATTTTGCAAG+AGG + chr1.1:78359770-78359789 MS.gene76296:intron 25.0%
!! TATTTGAGATAATCTTGATC+TGG - chr1.1:78358234-78358253 None:intergenic 25.0%
!! TGATTTTCAATACCTATCAT+TGG + chr1.1:78358999-78359018 MS.gene76296:CDS 25.0%
!!! ATGTTTTGTTAGTCTTGTAA+AGG - chr1.1:78358728-78358747 None:intergenic 25.0%
!!! CTACGTTATGATTTTGATTT+TGG + chr1.1:78358359-78358378 MS.gene76296:intron 25.0%
!!! GATTTTAATCCATCAAAGTT+AGG - chr1.1:78359729-78359748 None:intergenic 25.0%
AAAGATAACAGTAGTCTTTG+AGG - chr1.1:78359446-78359465 None:intergenic 30.0%
AACATTGAAGCTTACAAGAT+TGG + chr1.1:78359379-78359398 MS.gene76296:CDS 30.0%
AAGACTACTGTTATCTTTCA+AGG + chr1.1:78359448-78359467 MS.gene76296:CDS 30.0%
AAGATAACAGTAGTCTTTGA+GGG - chr1.1:78359445-78359464 None:intergenic 30.0%
AATAAGAGAATTGAGGTTTG+TGG + chr1.1:78359281-78359300 MS.gene76296:intron 30.0%
ACATTGAAGCTTACAAGATT+GGG + chr1.1:78359380-78359399 MS.gene76296:CDS 30.0%
AGTATGAAGTTTCAATAGTG+TGG + chr1.1:78358873-78358892 MS.gene76296:CDS 30.0%
CAACTTCAATAAGAGAATTG+AGG + chr1.1:78359274-78359293 MS.gene76296:intron 30.0%
CAAGAATGTGATCTATCTAA+AGG + chr1.1:78358111-78358130 MS.gene76296:CDS 30.0%
GGAAAAAGATCATGTTTGTT+GGG + chr1.1:78358629-78358648 MS.gene76296:CDS 30.0%
GTATGAAGTTTCAATAGTGT+GGG + chr1.1:78358874-78358893 MS.gene76296:CDS 30.0%
GTCGCATTATTATGTGTTAT+AGG + chr1.1:78358850-78358869 MS.gene76296:intron 30.0%
TCTTTCTATGTTCAGATTTC+TGG + chr1.1:78358747-78358766 MS.gene76296:CDS 30.0%
TGATCTATCTAAAGGAGAAT+GGG + chr1.1:78358119-78358138 MS.gene76296:CDS 30.0%
TGTATATTTGCAAGTGATGT+TGG + chr1.1:78358560-78358579 MS.gene76296:intron 30.0%
TTACTTTACAAACACAACTG+CGG + chr1.1:78360113-78360132 MS.gene76296:CDS 30.0%
! GAAAAAGATCATGTTTGTTG+GGG + chr1.1:78358630-78358649 MS.gene76296:CDS 30.0%
! GTAAAGGTGTAATTTGATTC+TGG - chr1.1:78358712-78358731 None:intergenic 30.0%
! TTTTCAATACCTATCATTGG+TGG + chr1.1:78359002-78359021 MS.gene76296:CDS 30.0%
AACTAACCAAAAGCAAACAG+TGG - chr1.1:78359647-78359666 None:intergenic 35.0%
ACACACATTCGTTCTTCTAT+GGG + chr1.1:78358056-78358075 MS.gene76296:CDS 35.0%
ACTCTATAAGCGACAATATG+TGG + chr1.1:78358653-78358672 MS.gene76296:CDS 35.0%
ATCAATCCAATTAGACCAAG+TGG - chr1.1:78359410-78359429 None:intergenic 35.0%
GAATTGAGGTTTGTGGATAT+TGG + chr1.1:78359288-78359307 MS.gene76296:intron 35.0%
GGATTTGATTGTCTCAGAAA+TGG + chr1.1:78358204-78358223 MS.gene76296:CDS 35.0%
GGGAAAAAGATCATGTTTGT+TGG + chr1.1:78358628-78358647 MS.gene76296:CDS 35.0%
GTGATCTATCTAAAGGAGAA+TGG + chr1.1:78358118-78358137 MS.gene76296:CDS 35.0%
TCAAATCCCTGCACTATAAA+AGG - chr1.1:78358192-78358211 None:intergenic 35.0%
! AAGAAACCACTGTTTGCTTT+TGG + chr1.1:78359638-78359657 MS.gene76296:intron 35.0%
! CAAACATGATCTTTTTCCCT+CGG - chr1.1:78358627-78358646 None:intergenic 35.0%
! CAATATGACCAACTTTTGCA+TGG - chr1.1:78359683-78359702 None:intergenic 35.0%
!! GTTTGATGGTGTGAAATTCT+TGG + chr1.1:78358594-78358613 MS.gene76296:CDS 35.0%
!! GTTTTAGTCCATGCAAAAGT+TGG + chr1.1:78359672-78359691 MS.gene76296:intron 35.0%
!!! ATGCTGTATGCTGTTTTACT+AGG + chr1.1:78360232-78360251 MS.gene76296:CDS 35.0%
AAATTCTTGGAGACATACCG+AGG + chr1.1:78358607-78358626 MS.gene76296:CDS 40.0%
AACTTGACTCCGTTTCATCT+CGG + chr1.1:78358954-78358973 MS.gene76296:CDS 40.0%
AATTAGGAAAGAGTGGAGAG+TGG - chr1.1:78358313-78358332 None:intergenic 40.0%
AATTCTTGGAGACATACCGA+GGG + chr1.1:78358608-78358627 MS.gene76296:CDS 40.0%
ACAAGATTGGGTTAACCACT+TGG + chr1.1:78359392-78359411 MS.gene76296:CDS 40.0%
ACGGTTCGTGATAGAGAAAA+GGG + chr1.1:78358920-78358939 MS.gene76296:CDS 40.0%
AGCATTTCATTCCAAGTGTC+GGG - chr1.1:78360217-78360236 None:intergenic 40.0%
CAATAGTGTGGGTGAAAGAT+GGG + chr1.1:78358885-78358904 MS.gene76296:CDS 40.0%
CACACACATTCGTTCTTCTA+TGG + chr1.1:78358055-78358074 MS.gene76296:CDS 40.0%
CAGCATTTCATTCCAAGTGT+CGG - chr1.1:78360218-78360237 None:intergenic 40.0%
CATAGAAGAACGAATGTGTG+TGG - chr1.1:78358057-78358076 None:intergenic 40.0%
CTCGACCAGTGAATATAGAA+GGG - chr1.1:78360150-78360169 None:intergenic 40.0%
CTTGGAAGATCACATGCAAA+AGG - chr1.1:78358267-78358286 None:intergenic 40.0%
GAACAAACTAAACCAGAGGA+AGG + chr1.1:78360010-78360029 MS.gene76296:CDS 40.0%
GCATCATAAAGAGGGTAGTA+TGG - chr1.1:78358156-78358175 None:intergenic 40.0%
GGTTAACCACTTGGTCTAAT+TGG + chr1.1:78359401-78359420 MS.gene76296:CDS 40.0%
TACGGTTCGTGATAGAGAAA+AGG + chr1.1:78358919-78358938 MS.gene76296:CDS 40.0%
TATAAAAGGACAGTCCCTTG+AGG - chr1.1:78358178-78358197 None:intergenic 40.0%
TCAATAGTGTGGGTGAAAGA+TGG + chr1.1:78358884-78358903 MS.gene76296:CDS 40.0%
TGTGTGAAACCACCAATGAT+AGG - chr1.1:78359014-78359033 None:intergenic 40.0%
TTCAAGGGAAAAACACTGTC+TGG + chr1.1:78359988-78360007 MS.gene76296:intron 40.0%
TTCTGAACAATGTCTACACC+AGG - chr1.1:78360052-78360071 None:intergenic 40.0%
! ATCCACAGACACTTTCAAGT+GGG + chr1.1:78359331-78359350 MS.gene76296:intron 40.0%
! CTTTTGCATGTGATCTTCCA+AGG + chr1.1:78358265-78358284 MS.gene76296:CDS 40.0%
! GACTGTCCTTTTATAGTGCA+GGG + chr1.1:78358183-78358202 MS.gene76296:CDS 40.0%
! TATCATTGGTGGTTTCACAC+AGG + chr1.1:78359013-78359032 MS.gene76296:CDS 40.0%
! TCTGGAACAAACTAAACCAG+AGG + chr1.1:78360006-78360025 MS.gene76296:CDS 40.0%
ACCCTCTTTATGATGCCTCA+AGG + chr1.1:78358160-78358179 MS.gene76296:CDS 45.0%
ATGTCTACACCAGGATATGC+TGG - chr1.1:78360043-78360062 None:intergenic 45.0%
ATTCGTTCTTCTATGGGCAG+TGG + chr1.1:78358062-78358081 MS.gene76296:CDS 45.0%
CAAACACAACTGCGGATAGA+TGG + chr1.1:78360121-78360140 MS.gene76296:CDS 45.0%
CATCCACAGACACTTTCAAG+TGG + chr1.1:78359330-78359349 MS.gene76296:intron 45.0%
CCACTTGTACCGAGATGAAA+CGG - chr1.1:78358966-78358985 None:intergenic 45.0%
CCCTCTTTATGATGCCTCAA+GGG + chr1.1:78358161-78358180 MS.gene76296:CDS 45.0%
CCTCGACCAGTGAATATAGA+AGG - chr1.1:78360151-78360170 None:intergenic 45.0%
CCTTCTATATTCACTGGTCG+AGG + chr1.1:78360148-78360167 MS.gene76296:CDS 45.0%
GAATTGCTGCTGCACATTCA+GGG + chr1.1:78359470-78359489 MS.gene76296:CDS 45.0%
GCATGCACATAGTACAGTGT+AGG - chr1.1:78358090-78358109 None:intergenic 45.0%
GGTCACCCTTCTATATTCAC+TGG + chr1.1:78360142-78360161 MS.gene76296:CDS 45.0%
TATCACGAACCGTATCCACT+AGG - chr1.1:78358913-78358932 None:intergenic 45.0%
TCATCTCGGTACAAGTGGAA+TGG + chr1.1:78358968-78358987 MS.gene76296:CDS 45.0%
TGGTCGAGGAACTTCTTATG+AGG + chr1.1:78360162-78360181 MS.gene76296:CDS 45.0%
! AGATGGGTACCTAGTGGATA+CGG + chr1.1:78358901-78358920 MS.gene76296:CDS 45.0%
! CCCTTGAGGCATCATAAAGA+GGG - chr1.1:78358164-78358183 None:intergenic 45.0%
! GGACTGTCCTTTTATAGTGC+AGG + chr1.1:78358182-78358201 MS.gene76296:CDS 45.0%
! TCCCTTGAGGCATCATAAAG+AGG - chr1.1:78358165-78358184 None:intergenic 45.0%
!! TTCCCACTTGAAAGTGTCTG+TGG - chr1.1:78359336-78359355 None:intergenic 45.0%
!! AATAAAAAATGATATATTTA+TGG - chr1.1:78359530-78359549 None:intergenic 5.0%
!! ATAAAAAATGATATATTTAT+GGG - chr1.1:78359529-78359548 None:intergenic 5.0%
!!! TAAAAATCATATTTAAAATT+AGG - chr1.1:78358329-78358348 None:intergenic 5.0%
CCGTTTCATCTCGGTACAAG+TGG + chr1.1:78358963-78358982 MS.gene76296:CDS 50.0%
CTTATGAGGACTGCAGTCAC+TGG + chr1.1:78360176-78360195 MS.gene76296:CDS 50.0%
GGAATTGCTGCTGCACATTC+AGG + chr1.1:78359469-78359488 MS.gene76296:CDS 50.0%
GGATCAAAGCCAGCATATCC+TGG + chr1.1:78360031-78360050 MS.gene76296:CDS 50.0%
GGTGAAAGATGGGTACCTAG+TGG + chr1.1:78358895-78358914 MS.gene76296:CDS 50.0%
TGCAGTCACTGGTGTCTAGC+TGG + chr1.1:78360187-78360206 MS.gene76296:CDS 55.0%
!! GCTGGCTTTGATCCTTCCTC+TGG - chr1.1:78360025-78360044 None:intergenic 55.0%
TAGCTGGTGCTCCCGACACT+TGG + chr1.1:78360203-78360222 MS.gene76296:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 78357988 78360279 78357988 ID=MS.gene76296
chr1.1 mRNA 78357988 78360279 78357988 ID=MS.gene76296.t1;Parent=MS.gene76296
chr1.1 exon 78357988 78358286 78357988 ID=MS.gene76296.t1.exon1;Parent=MS.gene76296.t1
chr1.1 CDS 78357988 78358286 78357988 ID=cds.MS.gene76296.t1;Parent=MS.gene76296.t1
chr1.1 exon 78358594 78358768 78358594 ID=MS.gene76296.t1.exon2;Parent=MS.gene76296.t1
chr1.1 CDS 78358594 78358768 78358594 ID=cds.MS.gene76296.t1;Parent=MS.gene76296.t1
chr1.1 exon 78358872 78359047 78358872 ID=MS.gene76296.t1.exon3;Parent=MS.gene76296.t1
chr1.1 CDS 78358872 78359047 78358872 ID=cds.MS.gene76296.t1;Parent=MS.gene76296.t1
chr1.1 exon 78359339 78359491 78359339 ID=MS.gene76296.t1.exon4;Parent=MS.gene76296.t1
chr1.1 CDS 78359339 78359491 78359339 ID=cds.MS.gene76296.t1;Parent=MS.gene76296.t1
chr1.1 exon 78359994 78360279 78359994 ID=MS.gene76296.t1.exon5;Parent=MS.gene76296.t1
chr1.1 CDS 78359994 78360279 78359994 ID=cds.MS.gene76296.t1;Parent=MS.gene76296.t1
Gene Sequence

>MS.gene76296

ATGGTTTGGTTTGGAAAGATTTTAAGACAATATCAAATGACATTGGATTTCTTGTTACATCTTCTGCCACACACATTCGTTCTTCTATGGGCAGTGGCACCTACACTGTACTATGTGCATGCTCAAGAATGTGATCTATCTAAAGGAGAATGGGTTGTTGATGATCCATACTACCCTCTTTATGATGCCTCAAGGGACTGTCCTTTTATAGTGCAGGGATTTGATTGTCTCAGAAATGGTAGACCAGATCAAGATTATCTCAAATATAGATGGAAACCTTTTGCATGTGATCTTCCAAGGTAATAATAATGTTTCATTGTTTCCACTCTCCACTCTTTCCTAATTTTAAATATGATTTTTAGTCATGTATACTACGTTATGATTTTGATTTTGGTCCTTGTTATTTTGTATTAGTTGATGCAATATTTATTTTTATTGGAATTGATCCTTATAATATGTAAAATGTTTGATTTTAGTACCACTAAATTAAGACTAAAATAAATATTATCGACCAATTTCAATATGAACAAATTTTGCAAGAACTAAAACAAAATACCTAAGACAAAACTAATTGTATATTTGCAAGTGATGTTGGTTAATTAATAGGTTTGATGGTGTGAAATTCTTGGAGACATACCGAGGGAAAAAGATCATGTTTGTTGGGGACTCTATAAGCGACAATATGTGGCAGTCACTTGCTTGTTTGCTTCACATTGCAGTGCCAGAATCAAATTACACCTTTACAAGACTAACAAAACATCTTTCTATGTTCAGATTTCTGGTAGTTTTCTTCAACTCCAATCTTAAATTAAAGGAAAATTTTGATCCTGATAATTGTTTGATAAGTTATCTTGGTAACATTGTCGCATTATTATGTGTTATAGGAGTATGAAGTTTCAATAGTGTGGGTGAAAGATGGGTACCTAGTGGATACGGTTCGTGATAGAGAAAAGGGAAGAATTATAAAACTTGACTCCGTTTCATCTCGGTACAAGTGGAATGGAGATGTTTTGATTTTCAATACCTATCATTGGTGGTTTCACACAGGACAGACTCCAATGTACTAAAGTTTTTTAGCTATATATTTAATAAAATGAAATAGATTAGTAACACTTTCTTCGAGACTCGTTTTGTGATTTAGTGAGTATTAGAGAGAGTTATTAGAATATAAGGTTAGTTATACTAGTTAGTTTTTTTTTCTTATTGGCTATAAGTCTTTATGTACCGAATGATAAGAAGAACAAACTAGTGTAACTAGCTTTTAAGTATCAAATAAGAGTGCTCAACAACTTCAATAAGAGAATTGAGGTTTGTGGATATTGGCTAATGTTTGTATGTTAATCATCCACAGACACTTTCAAGTGGGAAATGAGATAATGAAAGACATGGATAACATTGAAGCTTACAAGATTGGGTTAACCACTTGGTCTAATTGGATTGATTCTAACATTGATCCCTCAAAGACTACTGTTATCTTTCAAGGAATTGCTGCTGCACATTCAGGGTAAGTTTAATTTAGATTTAAATATGTTTAATTTCCCATAAATATATCATTTTTTATTTTTTATTTCTATTAAAAAAAATTAGATAAGCTCTTTTAAAAAAATTATTTTAATCCTTGTAAATATACCATTTTTTTTTTAAAATGAGAAGAAACCACTGTTTGCTTTTGGTTAGTTATTTTGTTTTAGTCCATGCAAAAGTTGGTCATATTGAGATCAGTAATATTAATTTTAATCCTAACTTTGATGGATTAAAATCAAATATTCTATATCAATTTAGATAAGAACAAATTTTGCAAGAGGTATACTATAAAACAACAAGAATCAAATGTAAAAACCGTATATTTGCAAAGACTAAAATCAAAACAATATTTATACAAAGACTAAATTACAAAAATGACACTATTTGAAGAGATAAAAAAAACATATTTAAATCTTTAATTTATAATCCCATTTTAACTATAAAATTCACACTAAACTGATAATGCTTCATAAATTTTCAAGGGAAAAACACTGTCTGGAACAAACTAAACCAGAGGAAGGATCAAAGCCAGCATATCCTGGTGTAGACATTGTTCAGAATGTTTTGAGTAACATGAAAAGTCATGTTTATTGGCTTGACATTACTTTACAAACACAACTGCGGATAGATGGTCACCCTTCTATATTCACTGGTCGAGGAACTTCTTATGAGGACTGCAGTCACTGGTGTCTAGCTGGTGCTCCCGACACTTGGAATGAAATGCTGTATGCTGTTTTACTAGGAAATCAATATTATCGTCATTTGTAA
Protein sequence

>MS.gene76296.t1

MVWFGKILRQYQMTLDFLLHLLPHTFVLLWAVAPTLYYVHAQECDLSKGEWVVDDPYYPLYDASRDCPFIVQGFDCLRNGRPDQDYLKYRWKPFACDLPRFDGVKFLETYRGKKIMFVGDSISDNMWQSLACLLHIAVPESNYTFTRLTKHLSMFRFLEYEVSIVWVKDGYLVDTVRDREKGRIIKLDSVSSRYKWNGDVLIFNTYHWWFHTGQTPIHFQVGNEIMKDMDNIEAYKIGLTTWSNWIDSNIDPSKTTVIFQGIAAAHSGEKHCLEQTKPEEGSKPAYPGVDIVQNVLSNMKSHVYWLDITLQTQLRIDGHPSIFTGRGTSYEDCSHWCLAGAPDTWNEMLYAVLLGNQYYRHL