AlfalfaGEDB | MS.gene76515

M. sativa cultivar XinJiangDaYe / MS.gene76515

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76515.t1	XP_024633177.1	97.3	111	3	0	1	111	1	111	4.40E-49	203.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76515.t1	Q9M817	50.5	97	48	0	15	111	9	105	2.2e-20	99.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76515.t1	Q2HU41	97.3	111	3	0	1	111	1	111	3.2e-49	203.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04991	MS.gene76515	-0.808204	3.47E-50	-1.69E-46
MS.gene051122	MS.gene76515	0.815968	7.04E-52	-1.69E-46
MS.gene051184	MS.gene76515	0.812957	3.26E-51	-1.69E-46
MS.gene051987	MS.gene76515	0.822811	1.94E-53	-1.69E-46
MS.gene052580	MS.gene76515	0.800437	1.44E-48	-1.69E-46
MS.gene055441	MS.gene76515	0.810689	1.02E-50	-1.69E-46
MS.gene055442	MS.gene76515	0.804442	2.16E-49	-1.69E-46
MS.gene056849	MS.gene76515	0.866721	2.32E-65	-1.69E-46
MS.gene060084	MS.gene76515	0.815167	1.06E-51	-1.69E-46
MS.gene06010	MS.gene76515	0.814428	1.55E-51	-1.69E-46
MS.gene060297	MS.gene76515	0.804698	1.91E-49	-1.69E-46
MS.gene060851	MS.gene76515	0.811551	6.62E-51	-1.69E-46
MS.gene060852	MS.gene76515	0.81238	4.36E-51	-1.69E-46
MS.gene060870	MS.gene76515	0.810985	8.78E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76515.t1	MTR_2g101560	97.297	111	3	0	1	111	1	111	2.20e-70	221
MS.gene76515.t1	MTR_2g101610	87.387	111	14	0	1	111	1	111	3.75e-62	200
MS.gene76515.t1	MTR_2g101590	83.784	111	17	1	1	111	1	110	1.01e-57	188
MS.gene76515.t1	MTR_2g101640	81.982	111	16	2	1	111	1	107	5.99e-55	181
MS.gene76515.t1	MTR_1g057460	68.468	111	29	2	1	111	24	128	2.68e-43	149
MS.gene76515.t1	MTR_4g011280	57.627	118	41	4	1	111	1	116	3.72e-37	132
MS.gene76515.t1	MTR_2g101650	55.752	113	43	3	1	111	1	108	1.38e-34	125
MS.gene76515.t1	MTR_1g026740	47.959	98	48	1	14	111	7	101	1.43e-25	99.8
MS.gene76515.t1	MTR_1g026750	51.087	92	42	1	14	105	8	96	4.96e-25	98.2
MS.gene76515.t1	MTR_1g057460	73.684	57	15	0	55	111	1	57	7.55e-22	89.4
MS.gene76515.t1	MTR_1g009200	43.243	111	59	1	5	111	1	111	8.06e-22	89.4
MS.gene76515.t1	MTR_1g026770	48.235	85	41	1	15	99	6	87	1.93e-21	88.2
MS.gene76515.t1	MTR_0236s0050	71.930	57	16	0	55	111	1	57	2.45e-20	85.1
MS.gene76515.t1	MTR_8g103233	41.667	96	56	0	16	111	6	101	3.23e-20	84.7
MS.gene76515.t1	MTR_7g088820	40.000	95	57	0	14	108	12	106	1.54e-18	80.1
MS.gene76515.t1	MTR_7g088825	41.304	92	54	0	17	108	12	103	3.07e-18	79.0
MS.gene76515.t1	MTR_5g038190	42.105	95	52	2	14	106	10	103	8.56e-18	77.8
MS.gene76515.t1	MTR_8g469470	35.000	100	63	1	14	111	17	116	2.14e-17	76.6
MS.gene76515.t1	MTR_7g064160	32.143	112	66	1	11	112	3	114	3.51e-17	71.6
MS.gene76515.t1	MTR_5g038060	37.113	97	61	0	15	111	19	115	7.97e-17	75.1
MS.gene76515.t1	MTR_7g088790	38.298	94	58	0	15	108	17	110	2.76e-16	73.6
MS.gene76515.t1	MTR_5g055000	41.667	84	49	0	25	108	16	99	5.87e-16	72.4
MS.gene76515.t1	MTR_7g064800	36.782	87	55	0	25	111	24	110	1.68e-15	71.2
MS.gene76515.t1	MTR_4g107580	32.039	103	69	1	9	111	7	108	8.76e-15	69.3
MS.gene76515.t1	MTR_7g064780	31.481	108	64	1	14	111	3	110	2.81e-14	67.8
MS.gene76515.t1	MTR_4g107510	32.673	101	68	0	11	111	20	120	6.17e-14	66.6
MS.gene76515.t1	MTR_8g469480	39.024	82	50	0	30	111	41	122	1.14e-13	65.9
MS.gene76515.t1	MTR_4g107610	32.653	98	66	0	14	111	18	115	1.47e-13	65.9
MS.gene76515.t1	MTR_2g093180	34.343	99	59	2	1	99	1	93	2.18e-12	62.4
MS.gene76515.t1	MTR_4g107530	30.097	103	67	1	14	111	15	117	5.34e-12	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76515.t1	AT1G52190	50.515	97	48	0	15	111	9	105	8.27e-29	108
MS.gene76515.t1	AT3G16180	50.000	102	50	1	11	111	6	107	1.75e-25	99.8
MS.gene76515.t1	AT5G11570	54.878	82	37	0	28	109	14	95	1.39e-20	85.5
MS.gene76515.t1	AT1G68570	43.000	100	57	0	9	108	5	104	1.29e-19	83.2
MS.gene76515.t1	AT1G69860	40.230	87	52	0	25	111	16	102	1.57e-15	71.2
MS.gene76515.t1	AT1G69870	34.043	94	62	0	18	111	42	135	2.95e-14	67.8
MS.gene76515.t1	AT5G62680	39.252	107	63	2	9	114	25	130	1.51e-13	65.9
MS.gene76515.t1	AT5G28470	36.782	87	55	0	25	111	15	101	6.35e-13	63.9
MS.gene76515.t1	AT5G28470	36.782	87	55	0	25	111	15	101	6.67e-13	63.9
MS.gene76515.t1	AT1G18880	36.585	82	52	0	30	111	22	103	6.90e-13	63.9
MS.gene76515.t1	AT1G27080	32.955	88	59	0	24	111	11	98	7.89e-13	63.5
MS.gene76515.t1	AT3G47960	35.238	105	62	2	10	114	50	148	1.50e-11	60.1

Find 23 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTATTGCAGATTCTTATAT+TGG	0.292916	2.3:-2532877	MS.gene76515:CDS
GATAAGATACCAATGATAAA+AGG	0.324542	2.3:+2533144	None:intergenic
AGGTTCTATTATCAGTTTCC+TGG	0.349967	2.3:-2532833	MS.gene76515:intron
CAACATGATATTGTATTTGA+TGG	0.361317	2.3:-2532986	MS.gene76515:CDS
AGACCACCCTTGCTTCTTTG+TGG	0.395960	2.3:+2533177	None:intergenic
CGATTCCTAGGTGTTGGAAT+AGG	0.398985	2.3:-2532853	MS.gene76515:CDS
TCTTATATTGGTCGATTCCT+AGG	0.404111	2.3:-2532865	MS.gene76515:CDS
ATCAAATACAATATCATGTT+GGG	0.422265	2.3:+2532988	None:intergenic
TGCTGCTGATGAAGAAGAAA+TGG	0.464327	2.3:-2533214	MS.gene76515:CDS
GCAATAAAAGCAGCAACAAC+AGG	0.471001	2.3:+2532892	None:intergenic
ATTGGTCGATTCCTAGGTGT+TGG	0.484458	2.3:-2532859	MS.gene76515:CDS
AGGATGGCAAGTGCTGGACT+TGG	0.485050	2.3:-2533012	MS.gene76515:CDS
CTTGCTAGGATGGCAAGTGC+TGG	0.493484	2.3:-2533018	MS.gene76515:CDS
TAATGAGGCACTTGCTAGGA+TGG	0.505197	2.3:-2533028	MS.gene76515:CDS
CTTCTCATATCCTCTGCAGC+TGG	0.510191	2.3:-2532925	MS.gene76515:CDS
CATCAAATACAATATCATGT+TGG	0.518744	2.3:+2532987	None:intergenic
AACATGATATTGTATTTGAT+GGG	0.530924	2.3:-2532985	MS.gene76515:CDS
CAGCTAATGAGGCACTTGCT+AGG	0.560867	2.3:-2533032	MS.gene76515:CDS
GAGTAGCTGTAGCAAGATGA+AGG	0.565218	2.3:+2532953	None:intergenic
TGTGAAATTTCCAGCTGCAG+AGG	0.589353	2.3:+2532915	None:intergenic
GATACCAATGATAAAAGGCA+TGG	0.603181	2.3:+2533149	None:intergenic
AAACCACAAAGAAGCAAGGG+TGG	0.667497	2.3:-2533180	MS.gene76515:CDS
TAGAACCTATTCCAACACCT+AGG	0.705760	2.3:+2532848	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGATTAATTAAAGATATATT+AGG	-	chr2.3:2532974-2532993	MS.gene76515:CDS	10.0%
!!	TTCTTTCAAAAAAAAAAAAA+AGG	+	chr2.3:2532886-2532905	None:intergenic	10.0%
!!	AATGAATATATAATATTACC+AGG	+	chr2.3:2532575-2532594	None:intergenic	15.0%
!!	ATATTATTAAGTCCAATTAT+GGG	-	chr2.3:2532657-2532676	MS.gene76515:intron	15.0%
!!	CTCAAATATTTAATTGATAT+AGG	+	chr2.3:2533189-2533208	None:intergenic	15.0%
!!	AACATGATATTGTATTTGAT+GGG	-	chr2.3:2532402-2532421	MS.gene76515:intron	20.0%
!!	ATCAAATACAATATCATGTT+GGG	+	chr2.3:2532402-2532421	None:intergenic	20.0%
!!	CATATTATTAAGTCCAATTA+TGG	-	chr2.3:2532656-2532675	MS.gene76515:intron	20.0%
!!	TTTATTGCAGATTCTTATAT+TGG	-	chr2.3:2532510-2532529	MS.gene76515:intron	20.0%
!!!	AAAAGATAAGAAAAGTATCA+TGG	+	chr2.3:2532624-2532643	None:intergenic	20.0%
!!!	CTTTATTTTTACTTTGAATG+AGG	-	chr2.3:2532292-2532311	MS.gene76515:intron	20.0%
!	ATACAATTTACTCCCATAAT+TGG	+	chr2.3:2532672-2532691	None:intergenic	25.0%
!	CAACATGATATTGTATTTGA+TGG	-	chr2.3:2532401-2532420	MS.gene76515:intron	25.0%
!	CATCAAATACAATATCATGT+TGG	+	chr2.3:2532403-2532422	None:intergenic	25.0%
!	GATAAGATACCAATGATAAA+AGG	+	chr2.3:2532246-2532265	None:intergenic	25.0%
!	GTATCATGGAATGAAAAAAA+AGG	+	chr2.3:2532610-2532629	None:intergenic	25.0%
!	TCAAAATGTGACAATTCAAA+AGG	+	chr2.3:2532751-2532770	None:intergenic	25.0%
!	TGCAAAAGTGACAATTATAA+AGG	-	chr2.3:2532790-2532809	MS.gene76515:intron	25.0%
	AATCAATATGTTGTAGATCC+AGG	+	chr2.3:2532923-2532942	None:intergenic	30.0%
	AGAAAGAATCACAAATCAAG+GGG	+	chr2.3:2533085-2533104	None:intergenic	30.0%
	GAACACTTGAATTGAATCTA+TGG	-	chr2.3:2532996-2533015	MS.gene76515:CDS	30.0%
	GACAATTATAAAGGAACTGA+GGG	-	chr2.3:2532799-2532818	MS.gene76515:intron	30.0%
	GAGAAAGAATCACAAATCAA+GGG	+	chr2.3:2533086-2533105	None:intergenic	30.0%
	TAATCATACTTCAATGCAGA+AGG	-	chr2.3:2532831-2532850	MS.gene76515:intron	30.0%
	TGACAATTATAAAGGAACTG+AGG	-	chr2.3:2532798-2532817	MS.gene76515:intron	30.0%
	TGAGAAAGAATCACAAATCA+AGG	+	chr2.3:2533087-2533106	None:intergenic	30.0%
	TTTGTGATTCTTTCTCAACT+AGG	-	chr2.3:2533090-2533109	MS.gene76515:intron	30.0%
!	AGAAGCATATATGTATAACC+TGG	-	chr2.3:2532902-2532921	MS.gene76515:CDS	30.0%
!	ATCTGAAACATAAAGAGAGA+CGG	-	chr2.3:2533041-2533060	MS.gene76515:intron	30.0%
	AATCTATGGCTAAAGTCTGT+GGG	-	chr2.3:2533010-2533029	MS.gene76515:CDS	35.0%
	ACAAAAACCACAAAGAAGCA+AGG	-	chr2.3:2532203-2532222	MS.gene76515:intron	35.0%
	AGGTTCTATTATCAGTTTCC+TGG	-	chr2.3:2532554-2532573	MS.gene76515:intron	35.0%
	ATTGTCTTGCCTAAGACATA+TGG	+	chr2.3:2533119-2533138	None:intergenic	35.0%
	CAAAAACCACAAAGAAGCAA+GGG	-	chr2.3:2532204-2532223	MS.gene76515:intron	35.0%
	GACAATTCAAAAGGAAGAGA+GGG	+	chr2.3:2532742-2532761	None:intergenic	35.0%
	GATACCAATGATAAAAGGCA+TGG	+	chr2.3:2532241-2532260	None:intergenic	35.0%
	TGACAATTCAAAAGGAAGAG+AGG	+	chr2.3:2532743-2532762	None:intergenic	35.0%
!	TCTTATATTGGTCGATTCCT+AGG	-	chr2.3:2532522-2532541	MS.gene76515:intron	35.0%
!!!	TTTTGTTGTTGCAGCTAATG+AGG	-	chr2.3:2532344-2532363	MS.gene76515:intron	35.0%
	ACTAGGACTCCATATGTCTT+AGG	-	chr2.3:2533107-2533126	MS.gene76515:intron	40.0%
	GAATCTATGGCTAAAGTCTG+TGG	-	chr2.3:2533009-2533028	MS.gene76515:CDS	40.0%
	GCAATAAAAGCAGCAACAAC+AGG	+	chr2.3:2532498-2532517	None:intergenic	40.0%
	TAGAACCTATTCCAACACCT+AGG	+	chr2.3:2532542-2532561	None:intergenic	40.0%
	TGCTGCTGATGAAGAAGAAA+TGG	-	chr2.3:2532173-2532192	MS.gene76515:intron	40.0%
!	GTCACCATGCCTTTTATCAT+TGG	-	chr2.3:2532234-2532253	MS.gene76515:intron	40.0%
	AAACCACAAAGAAGCAAGGG+TGG	-	chr2.3:2532207-2532226	MS.gene76515:intron	45.0%
	GAGTAGCTGTAGCAAGATGA+AGG	+	chr2.3:2532437-2532456	None:intergenic	45.0%
	TGTGAAATTTCCAGCTGCAG+AGG	+	chr2.3:2532475-2532494	None:intergenic	45.0%
!	ATTGGTCGATTCCTAGGTGT+TGG	-	chr2.3:2532528-2532547	MS.gene76515:intron	45.0%
!	TAATGAGGCACTTGCTAGGA+TGG	-	chr2.3:2532359-2532378	MS.gene76515:intron	45.0%
!!	CGATTCCTAGGTGTTGGAAT+AGG	-	chr2.3:2532534-2532553	MS.gene76515:intron	45.0%
!!!	TAATATTATATATTCATTTT+TGG	-	chr2.3:2532577-2532596	MS.gene76515:intron	5.0%
	AGACCACCCTTGCTTCTTTG+TGG	+	chr2.3:2532213-2532232	None:intergenic	50.0%
	CTTCTCATATCCTCTGCAGC+TGG	-	chr2.3:2532462-2532481	MS.gene76515:intron	50.0%
!	CAGCTAATGAGGCACTTGCT+AGG	-	chr2.3:2532355-2532374	MS.gene76515:intron	50.0%
	CTTGCTAGGATGGCAAGTGC+TGG	-	chr2.3:2532369-2532388	MS.gene76515:intron	55.0%
!!	AGGATGGCAAGTGCTGGACT+TGG	-	chr2.3:2532375-2532394	MS.gene76515:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	2532141	2533268	2532141	ID=MS.gene76515
chr2.3	mRNA	2532141	2533268	2532141	ID=MS.gene76515.t1;Parent=MS.gene76515
chr2.3	exon	2533154	2533268	2533154	ID=MS.gene76515.t1.exon1;Parent=MS.gene76515.t1
chr2.3	CDS	2533154	2533268	2533154	ID=cds.MS.gene76515.t1;Parent=MS.gene76515.t1
chr2.3	exon	2532834	2533051	2532834	ID=MS.gene76515.t1.exon2;Parent=MS.gene76515.t1
chr2.3	CDS	2532834	2533051	2532834	ID=cds.MS.gene76515.t1;Parent=MS.gene76515.t1
chr2.3	exon	2532141	2532152	2532141	ID=MS.gene76515.t1.exon3;Parent=MS.gene76515.t1
chr2.3	CDS	2532141	2532152	2532141	ID=cds.MS.gene76515.t1;Parent=MS.gene76515.t1

Gene Sequence

>MS.gene76515

ATGGATAAGGAACTTCAACTTTGTTCAGTTGATGCTGCTGATGAAGAAGAAATGGTTTCTCAACAAAAACCACAAAGAAGCAAGGGTGGTCTTGTCACCATGCCTTTTATCATTGGTATCTTATCTATAACTTGCATGCATTTTTATTTTACTTTATTTTTACTTTGAATGAGGTTGAATTTTGATGTATAATATTAATATGATTTTGTTGTTGCAGCTAATGAGGCACTTGCTAGGATGGCAAGTGCTGGACTTGGACCCAACATGATATTGTATTTGATGGGATCTTACAACCTTCATCTTGCTACAGCTACTCAGATACTTCTCATATCCTCTGCAGCTGGAAATTTCACACCTGTTGTTGCTGCTTTTATTGCAGATTCTTATATTGGTCGATTCCTAGGTGTTGGAATAGGTTCTATTATCAGTTTCCTGGTAATATTATATATTCATTTTTGGTTTACTTCCTTTTTTTTCATTCCATGATACTTTTCTTATCTTTTGACTTGAATATGCATATTATTAAGTCCAATTATGGGAGTAAATTGTATTTGAATTAATCACATACATAGAAGACATGCTCAATATAAATATTACACCCTCTCTTCCTTTTGAATTGTCACATTTTGACTTAGAAACAAAAATTTTATGCAAAAGTGACAATTATAAAGGAACTGAGGGAGTACACTTTAATCATACTTCAATGCAGAAGGAAAATAAAAAGATAAGCATATATGTATAACCTTTTTTTTTTTTTTGAAAGAAGCATATATGTATAACCTGGATCTACAACATATTGATTTTTTTGTAATCAACAACAACATACAACATATTGATTAATTAAAGATATATTAGGAACACTTGAATTGAATCTATGGCTAAAGTCTGTGGGCGACAATGATCTGAAACATAAAGAGAGACGGATAATGTCATAACGAATGCCCCTTGATTTGTGATTCTTTCTCAACTAGGACTCCATATGTCTTAGGCAAGACAATACGCAGTGTGTCTGATATATTGAAGATCATTGTCTACATATATTGAACCTATATCAATTAAATATTTGAGTAGTTAAATAAAATATTCAACTATGATATTCTGATTAATTCTCCTCAGATTCCTTTTTAA

Protein sequence

>MS.gene76515.t1

MDKELQLCSVDAADEEEMVSQQKPQRSKGGLVTMPFIIANEALARMASAGLGPNMILYLMGSYNLHLATATQILLISSAAGNFTPVVAAFIADSYIGRFLGVGIGSIISFLIPF