Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78337.t1 | XP_003626325.2 | 90.3 | 145 | 10 | 2 | 1 | 145 | 24 | 164 | 7.00E-68 | 266.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78337.t1 | G7KZQ0 | 90.3 | 145 | 10 | 2 | 1 | 145 | 24 | 164 | 5.1e-68 | 266.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049341 | MS.gene78337 | 0.810722 | 1.00E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene78337 | 0.807975 | 3.89E-50 | -1.69E-46 |
| MS.gene050020 | MS.gene78337 | 0.832373 | 9.81E-56 | -1.69E-46 |
| MS.gene050438 | MS.gene78337 | 0.813533 | 2.44E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene78337 | 0.810837 | 9.45E-51 | -1.69E-46 |
| MS.gene050815 | MS.gene78337 | 0.819158 | 1.35E-52 | -1.69E-46 |
| MS.gene050959 | MS.gene78337 | 0.805774 | 1.13E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene78337 | 0.818508 | 1.89E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene78337 | 0.809648 | 1.71E-50 | -1.69E-46 |
| MS.gene052020 | MS.gene78337 | 0.816544 | 5.24E-52 | -1.69E-46 |
| MS.gene052133 | MS.gene78337 | 0.808064 | 3.72E-50 | -1.69E-46 |
| MS.gene052613 | MS.gene78337 | 0.806147 | 9.47E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene78337 | 0.830369 | 3.05E-55 | -1.69E-46 |
| MS.gene053221 | MS.gene78337 | 0.806939 | 6.45E-50 | -1.69E-46 |
| MS.gene053630 | MS.gene78337 | 0.800794 | 1.22E-48 | -1.69E-46 |
| MS.gene055512 | MS.gene78337 | 0.80279 | 4.75E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene78337 | 0.808404 | 3.15E-50 | -1.69E-46 |
| MS.gene058636 | MS.gene78337 | 0.820529 | 6.54E-53 | -1.69E-46 |
| MS.gene058906 | MS.gene78337 | 0.806015 | 1.01E-49 | -1.69E-46 |
| MS.gene059937 | MS.gene78337 | 0.817551 | 3.11E-52 | -1.69E-46 |
| MS.gene060587 | MS.gene78337 | 0.819673 | 1.03E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78337.t1 | MTR_7g113900 | 90.345 | 145 | 10 | 2 | 1 | 145 | 24 | 164 | 3.34e-86 | 249 |
| MS.gene78337.t1 | MTR_2g024090 | 40.132 | 152 | 81 | 3 | 1 | 142 | 16 | 167 | 1.38e-29 | 106 |
| MS.gene78337.t1 | MTR_4g050050 | 35.625 | 160 | 86 | 2 | 1 | 143 | 18 | 177 | 1.74e-28 | 103 |
| MS.gene78337.t1 | MTR_4g050050 | 36.364 | 143 | 75 | 2 | 1 | 143 | 18 | 144 | 4.62e-27 | 99.4 |
| MS.gene78337.t1 | MTR_2g450100 | 42.982 | 114 | 62 | 1 | 33 | 143 | 63 | 176 | 1.42e-23 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78337.t1 | AT1G05070 | 41.060 | 151 | 70 | 5 | 8 | 143 | 22 | 168 | 1.23e-31 | 111 |
| MS.gene78337.t1 | AT2G32580 | 37.662 | 154 | 84 | 3 | 1 | 143 | 15 | 167 | 4.85e-30 | 107 |
| MS.gene78337.t1 | AT4G04360 | 41.935 | 155 | 76 | 4 | 1 | 143 | 16 | 168 | 1.45e-28 | 103 |
| MS.gene78337.t1 | AT2G24290 | 39.873 | 158 | 80 | 3 | 1 | 143 | 15 | 172 | 2.74e-28 | 102 |
| MS.gene78337.t1 | AT4G30996 | 50.515 | 97 | 48 | 0 | 45 | 141 | 73 | 169 | 1.41e-27 | 100 |
| MS.gene78337.t1 | AT2G32580 | 43.000 | 100 | 57 | 0 | 44 | 143 | 6 | 105 | 1.58e-24 | 91.7 |
Find 40 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAATATCCCGTTGCTTCTT+TGG | 0.212587 | 7.1:+2627674 | MS.gene78337:CDS |
| TGCATTTATTACTAATCTTT+TGG | 0.212992 | 7.1:-2628484 | None:intergenic |
| GAAGAACGAGCTCGTGATTA+TGG | 0.234749 | 7.1:+2628633 | MS.gene78337:CDS |
| TTCATTTCCTCCTTCATTGT+TGG | 0.237361 | 7.1:-2628387 | None:intergenic |
| AGCGCAGGTTGACGGCATTA+TGG | 0.273292 | 7.1:+2628610 | MS.gene78337:CDS |
| CTGTTCCTCACTTGCCTCAC+TGG | 0.367272 | 7.1:+2627653 | MS.gene78337:CDS |
| TGTTACCTTGTGTTCACAAT+TGG | 0.371180 | 7.1:+2627573 | MS.gene78337:CDS |
| GAATATCCCGTTGCTTCTTT+GGG | 0.376906 | 7.1:+2627675 | MS.gene78337:CDS |
| GCGCAGGTTGACGGCATTAT+GGG | 0.391190 | 7.1:+2628611 | MS.gene78337:CDS |
| TTACTATATGTACAGATTGT+GGG | 0.418392 | 7.1:+2628355 | MS.gene78337:intron |
| GAAGTAGAAAAGTGCAGCAT+AGG | 0.426789 | 7.1:+2628534 | MS.gene78337:CDS |
| CAGAGCTTGTTGAAGAGCGC+AGG | 0.429709 | 7.1:+2628595 | MS.gene78337:CDS |
| AAGAACGAGCTCGTGATTAT+GGG | 0.441049 | 7.1:+2628634 | MS.gene78337:CDS |
| GGTCTGTTCCAAAGTCTCAT+TGG | 0.443502 | 7.1:-2628463 | None:intergenic |
| AATTGTAGCCAATGAGACTT+TGG | 0.447499 | 7.1:+2628455 | MS.gene78337:CDS |
| GTGATTATGGGTGGAAGGAC+AGG | 0.457853 | 7.1:+2628646 | MS.gene78337:CDS |
| AACTTACCCAAAGAAGCAAC+GGG | 0.499220 | 7.1:-2627681 | None:intergenic |
| TGTTGAAGAGCGCAGGTTGA+CGG | 0.500440 | 7.1:+2628602 | MS.gene78337:CDS |
| AAGTAGAAAAGTGCAGCATA+GGG | 0.518644 | 7.1:+2628535 | MS.gene78337:CDS |
| TTCTTCAAACGCCAGCGAGA+TGG | 0.541960 | 7.1:-2627600 | None:intergenic |
| AGAAAAGTGCAGCATAGGGA+TGG | 0.556983 | 7.1:+2628539 | MS.gene78337:CDS |
| CAATCATGACATTGAGGACA+AGG | 0.561176 | 7.1:-2627627 | None:intergenic |
| CAATTGGACCACCATCTCGC+TGG | 0.563845 | 7.1:+2627589 | MS.gene78337:CDS |
| TGGAAACTTGTGAAGAAGCA+AGG | 0.573605 | 7.1:+2628559 | MS.gene78337:CDS |
| TTCAAACGCCAGCGAGATGG+TGG | 0.580007 | 7.1:-2627597 | None:intergenic |
| AGAAGCAAGGGAGAGAGCAG+AGG | 0.585238 | 7.1:+2628572 | MS.gene78337:CDS |
| GAGACATGATCCAACAATGA+AGG | 0.590741 | 7.1:+2628377 | MS.gene78337:CDS |
| AGCTCGTGATTATGGGTGGA+AGG | 0.596678 | 7.1:+2628641 | MS.gene78337:CDS |
| AAAGATTAGTAATAAATGCA+AGG | 0.596837 | 7.1:+2628487 | MS.gene78337:CDS |
| TCCTCTGATAGCATTGTCAG+TGG | 0.601328 | 7.1:-2628417 | None:intergenic |
| AAACTTACCCAAAGAAGCAA+CGG | 0.604283 | 7.1:-2627682 | None:intergenic |
| AACGAGCTCGTGATTATGGG+TGG | 0.612321 | 7.1:+2628637 | MS.gene78337:CDS |
| GGAAACTTGTGAAGAAGCAA+GGG | 0.615984 | 7.1:+2628560 | MS.gene78337:CDS |
| CAGTAACAATCATGACATTG+AGG | 0.634830 | 7.1:-2627633 | None:intergenic |
| TCCACTGACAATGCTATCAG+AGG | 0.642154 | 7.1:+2628416 | MS.gene78337:CDS |
| ATATTCCAGTGAGGCAAGTG+AGG | 0.642772 | 7.1:-2627658 | None:intergenic |
| ACATGATCCAACAATGAAGG+AGG | 0.647322 | 7.1:+2628380 | MS.gene78337:CDS |
| GTGGTCCAATTGTGAACACA+AGG | 0.658093 | 7.1:-2627578 | None:intergenic |
| AATGAAGGAGGAAATGAACA+AGG | 0.667487 | 7.1:+2628392 | MS.gene78337:CDS |
| AGCAACGGGATATTCCAGTG+AGG | 0.784097 | 7.1:-2627667 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATAAATTTTACTTAAAG+TGG | - | chr7.1:2628213-2628232 | None:intergenic | 10.0% |
| !! | AAAGATTAGTAATAAATGCA+AGG | + | chr7.1:2628487-2628506 | MS.gene78337:CDS | 20.0% |
| !! | AGAAATGCAAAACATTTAAA+TGG | - | chr7.1:2628124-2628143 | None:intergenic | 20.0% |
| !! | CAATTCAATTCATATGTATT+AGG | + | chr7.1:2627904-2627923 | MS.gene78337:intron | 20.0% |
| !! | TAGGTTTATATATTAATTCG+TGG | + | chr7.1:2627923-2627942 | MS.gene78337:intron | 20.0% |
| !! | TAGTAATTAGAAAAATTCAC+TGG | + | chr7.1:2627817-2627836 | MS.gene78337:intron | 20.0% |
| !!! | TGCATTTATTACTAATCTTT+TGG | - | chr7.1:2628487-2628506 | None:intergenic | 20.0% |
| ! | ATTGAATAACCAAAACATTC+TGG | - | chr7.1:2627888-2627907 | None:intergenic | 25.0% |
| ! | ATTTGTAGAGTAAGTGAAAA+AGG | - | chr7.1:2627709-2627728 | None:intergenic | 25.0% |
| ! | TTACTATATGTACAGATTGT+GGG | + | chr7.1:2628355-2628374 | MS.gene78337:intron | 25.0% |
| ! | TTTACTATATGTACAGATTG+TGG | + | chr7.1:2628354-2628373 | MS.gene78337:intron | 25.0% |
| ! | TTTGTAGAGTAAGTGAAAAA+GGG | - | chr7.1:2627708-2627727 | None:intergenic | 25.0% |
| !!! | CATCATTTTTCAAACACAAA+GGG | - | chr7.1:2627794-2627813 | None:intergenic | 25.0% |
| ACAAGTTTGATTAGATGTAC+AGG | + | chr7.1:2627757-2627776 | MS.gene78337:intron | 30.0% | |
| ACTCTACAAATTAACAGACT+TGG | + | chr7.1:2627718-2627737 | MS.gene78337:intron | 30.0% | |
| ATAATGTCCAAAAGACAACA+TGG | - | chr7.1:2628316-2628335 | None:intergenic | 30.0% | |
| CTAAAAATGAGAAGAGAATG+AGG | - | chr7.1:2628273-2628292 | None:intergenic | 30.0% | |
| CTTTGTTCTGATTCTCAAAA+AGG | - | chr7.1:2627846-2627865 | None:intergenic | 30.0% | |
| TGATACAAAACATACATCGA+AGG | + | chr7.1:2628017-2628036 | MS.gene78337:intron | 30.0% | |
| ! | ATGTATGTTTTGTATCACAG+TGG | - | chr7.1:2628014-2628033 | None:intergenic | 30.0% |
| !! | GCATCATTTTTCAAACACAA+AGG | - | chr7.1:2627795-2627814 | None:intergenic | 30.0% |
| AAACTTACCCAAAGAAGCAA+CGG | - | chr7.1:2627685-2627704 | None:intergenic | 35.0% | |
| AATGAAGGAGGAAATGAACA+AGG | + | chr7.1:2628392-2628411 | MS.gene78337:CDS | 35.0% | |
| AATTGTAGCCAATGAGACTT+TGG | + | chr7.1:2628455-2628474 | MS.gene78337:CDS | 35.0% | |
| CAGTAACAATCATGACATTG+AGG | - | chr7.1:2627636-2627655 | None:intergenic | 35.0% | |
| TGTTACCTTGTGTTCACAAT+TGG | + | chr7.1:2627573-2627592 | MS.gene78337:CDS | 35.0% | |
| TTCATTTCCTCCTTCATTGT+TGG | - | chr7.1:2628390-2628409 | None:intergenic | 35.0% | |
| ! | AAGTAGAAAAGTGCAGCATA+GGG | + | chr7.1:2628535-2628554 | MS.gene78337:CDS | 35.0% |
| ! | TCTATCTAGCCAGAATGTTT+TGG | + | chr7.1:2627876-2627895 | MS.gene78337:intron | 35.0% |
| !! | CATAGCTAGCTTTGTTTATG+TGG | + | chr7.1:2628149-2628168 | MS.gene78337:intron | 35.0% |
| AACTTACCCAAAGAAGCAAC+GGG | - | chr7.1:2627684-2627703 | None:intergenic | 40.0% | |
| AAGAACGAGCTCGTGATTAT+GGG | + | chr7.1:2628634-2628653 | MS.gene78337:CDS | 40.0% | |
| ACATGATCCAACAATGAAGG+AGG | + | chr7.1:2628380-2628399 | MS.gene78337:CDS | 40.0% | |
| ATGTCCAAAAGACAACATGG+TGG | - | chr7.1:2628313-2628332 | None:intergenic | 40.0% | |
| CAATCATGACATTGAGGACA+AGG | - | chr7.1:2627630-2627649 | None:intergenic | 40.0% | |
| GAATATCCCGTTGCTTCTTT+GGG | + | chr7.1:2627675-2627694 | MS.gene78337:CDS | 40.0% | |
| GAGACATGATCCAACAATGA+AGG | + | chr7.1:2628377-2628396 | MS.gene78337:CDS | 40.0% | |
| GGAAACTTGTGAAGAAGCAA+GGG | + | chr7.1:2628560-2628579 | MS.gene78337:CDS | 40.0% | |
| TGGAAACTTGTGAAGAAGCA+AGG | + | chr7.1:2628559-2628578 | MS.gene78337:CDS | 40.0% | |
| ! | GAAGTAGAAAAGTGCAGCAT+AGG | + | chr7.1:2628534-2628553 | MS.gene78337:CDS | 40.0% |
| ! | TTCACCACCATGTTGTCTTT+TGG | + | chr7.1:2628306-2628325 | MS.gene78337:intron | 40.0% |
| ! | TTGTTTAGCTCAACACGGTA+CGG | + | chr7.1:2628047-2628066 | MS.gene78337:intron | 40.0% |
| !! | TTGCCTTGTTTAGCTCAACA+CGG | + | chr7.1:2628042-2628061 | MS.gene78337:intron | 40.0% |
| ATATTCCAGTGAGGCAAGTG+AGG | - | chr7.1:2627661-2627680 | None:intergenic | 45.0% | |
| GAAGAACGAGCTCGTGATTA+TGG | + | chr7.1:2628633-2628652 | MS.gene78337:CDS | 45.0% | |
| GGAATATCCCGTTGCTTCTT+TGG | + | chr7.1:2627674-2627693 | MS.gene78337:CDS | 45.0% | |
| GGTCTGTTCCAAAGTCTCAT+TGG | - | chr7.1:2628466-2628485 | None:intergenic | 45.0% | |
| GTACCGTGTTGAGCTAAACA+AGG | - | chr7.1:2628048-2628067 | None:intergenic | 45.0% | |
| GTGGTCCAATTGTGAACACA+AGG | - | chr7.1:2627581-2627600 | None:intergenic | 45.0% | |
| TCCACTGACAATGCTATCAG+AGG | + | chr7.1:2628416-2628435 | MS.gene78337:CDS | 45.0% | |
| TCCTCTGATAGCATTGTCAG+TGG | - | chr7.1:2628420-2628439 | None:intergenic | 45.0% | |
| ! | AGAAAAGTGCAGCATAGGGA+TGG | + | chr7.1:2628539-2628558 | MS.gene78337:CDS | 45.0% |
| ! | TGTTTAGCTCAACACGGTAC+GGG | + | chr7.1:2628048-2628067 | MS.gene78337:intron | 45.0% |
| AACGAGCTCGTGATTATGGG+TGG | + | chr7.1:2628637-2628656 | MS.gene78337:CDS | 50.0% | |
| AGCAACGGGATATTCCAGTG+AGG | - | chr7.1:2627670-2627689 | None:intergenic | 50.0% | |
| AGCTCGTGATTATGGGTGGA+AGG | + | chr7.1:2628641-2628660 | MS.gene78337:CDS | 50.0% | |
| GTGATTATGGGTGGAAGGAC+AGG | + | chr7.1:2628646-2628665 | MS.gene78337:CDS | 50.0% | |
| TTCTTCAAACGCCAGCGAGA+TGG | - | chr7.1:2627603-2627622 | None:intergenic | 50.0% | |
| ! | GTTTAGCTCAACACGGTACG+GGG | + | chr7.1:2628049-2628068 | MS.gene78337:intron | 50.0% |
| ! | TGTTGAAGAGCGCAGGTTGA+CGG | + | chr7.1:2628602-2628621 | MS.gene78337:CDS | 50.0% |
| AGAAGCAAGGGAGAGAGCAG+AGG | + | chr7.1:2628572-2628591 | MS.gene78337:CDS | 55.0% | |
| CAATTGGACCACCATCTCGC+TGG | + | chr7.1:2627589-2627608 | MS.gene78337:CDS | 55.0% | |
| CTGTTCCTCACTTGCCTCAC+TGG | + | chr7.1:2627653-2627672 | MS.gene78337:CDS | 55.0% | |
| TTCAAACGCCAGCGAGATGG+TGG | - | chr7.1:2627600-2627619 | None:intergenic | 55.0% | |
| ! | AGCGCAGGTTGACGGCATTA+TGG | + | chr7.1:2628610-2628629 | MS.gene78337:CDS | 55.0% |
| ! | GCGCAGGTTGACGGCATTAT+GGG | + | chr7.1:2628611-2628630 | MS.gene78337:CDS | 55.0% |
| !! | CAGAGCTTGTTGAAGAGCGC+AGG | + | chr7.1:2628595-2628614 | MS.gene78337:CDS | 55.0% |
| GCTCAACACGGTACGGGGAT+TGG | + | chr7.1:2628054-2628073 | MS.gene78337:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 2627564 | 2628674 | 2627564 | ID=MS.gene78337 |
| chr7.1 | mRNA | 2627564 | 2628674 | 2627564 | ID=MS.gene78337.t1;Parent=MS.gene78337 |
| chr7.1 | exon | 2627564 | 2627696 | 2627564 | ID=MS.gene78337.t1.exon1;Parent=MS.gene78337.t1 |
| chr7.1 | CDS | 2627564 | 2627696 | 2627564 | ID=cds.MS.gene78337.t1;Parent=MS.gene78337.t1 |
| chr7.1 | exon | 2628370 | 2628674 | 2628370 | ID=MS.gene78337.t1.exon2;Parent=MS.gene78337.t1 |
| chr7.1 | CDS | 2628370 | 2628674 | 2628370 | ID=cds.MS.gene78337.t1;Parent=MS.gene78337.t1 |
| Gene Sequence |
| Protein sequence |
