Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80182.t1 | AFK40772.1 | 87.2 | 187 | 7 | 1 | 1 | 170 | 1 | 187 | 1.50E-74 | 289.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80182.t1 | G7J9T9 | 87.2 | 187 | 7 | 1 | 1 | 170 | 1 | 187 | 1.1e-74 | 289.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene80182 | -0.81139 | 7.17E-51 | -1.69E-46 |
| MS.gene04917 | MS.gene80182 | -0.800756 | 1.24E-48 | -1.69E-46 |
| MS.gene04991 | MS.gene80182 | 0.862025 | 6.78E-64 | -1.69E-46 |
| MS.gene050488 | MS.gene80182 | 0.819298 | 1.25E-52 | -1.69E-46 |
| MS.gene050623 | MS.gene80182 | 0.80614 | 9.51E-50 | -1.69E-46 |
| MS.gene051122 | MS.gene80182 | -0.847977 | 8.22E-60 | -1.69E-46 |
| MS.gene05344 | MS.gene80182 | 0.845089 | 5.05E-59 | -1.69E-46 |
| MS.gene055060 | MS.gene80182 | 0.81515 | 1.07E-51 | -1.69E-46 |
| MS.gene055505 | MS.gene80182 | -0.812406 | 4.31E-51 | -1.69E-46 |
| MS.gene056849 | MS.gene80182 | -0.844567 | 6.98E-59 | -1.69E-46 |
| MS.gene057452 | MS.gene80182 | 0.804687 | 1.92E-49 | -1.69E-46 |
| MS.gene059872 | MS.gene80182 | 0.868234 | 7.60E-66 | -1.69E-46 |
| MS.gene060851 | MS.gene80182 | -0.825137 | 5.52E-54 | -1.69E-46 |
| MS.gene060852 | MS.gene80182 | -0.824438 | 8.07E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80182.t1 | MTR_3g105925 | 74.779 | 226 | 40 | 1 | 1 | 209 | 1 | 226 | 2.59e-116 | 330 |
| MS.gene80182.t1 | MTR_1g014130 | 33.918 | 171 | 60 | 5 | 1 | 152 | 1 | 137 | 7.41e-16 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80182.t1 | AT5G25080 | 47.191 | 178 | 75 | 2 | 13 | 173 | 16 | 191 | 6.87e-53 | 169 |
Find 47 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTGCTGCTCAGGAGTTTC+TGG | 0.118592 | 3.1:+83844897 | MS.gene80182:CDS |
| TGGTAATAATGGCGGTATTA+AGG | 0.185771 | 3.1:+83844944 | MS.gene80182:CDS |
| ACGCCTTCTAGATTTGAATA+AGG | 0.277390 | 3.1:+83844242 | MS.gene80182:CDS |
| CGCCTTCTAGATTTGAATAA+GGG | 0.349424 | 3.1:+83844243 | MS.gene80182:CDS |
| GGTAATAATGGCGGTATTAA+GGG | 0.353390 | 3.1:+83844945 | MS.gene80182:CDS |
| TTCTGTTCAAGCTGCTGCTC+AGG | 0.368572 | 3.1:+83844888 | MS.gene80182:CDS |
| GACTGAATCCATCACCGCTT+CGG | 0.381291 | 3.1:-83843794 | None:intergenic |
| GGAAGTTGGGAGAGAGTTTG+AGG | 0.381337 | 3.1:-83843883 | None:intergenic |
| AGAGTTTGAGGGTTCATAAG+AGG | 0.391223 | 3.1:-83843871 | None:intergenic |
| ACTGAATCCATCACCGCTTC+GGG | 0.404917 | 3.1:-83843793 | None:intergenic |
| GATTTGACAGGGTTATCATC+TGG | 0.415518 | 3.1:-83844097 | None:intergenic |
| CACGTTCAAGAGGTGGAAGT+TGG | 0.422478 | 3.1:-83843897 | None:intergenic |
| TGAACGGAATTCACGAAGAT+TGG | 0.426985 | 3.1:-83843830 | None:intergenic |
| TACCCTTATTCAAATCTAGA+AGG | 0.427243 | 3.1:-83844245 | None:intergenic |
| TGAGAATTCAACATAGTAGA+AGG | 0.433685 | 3.1:-83844603 | None:intergenic |
| TCTTTGCCTGACCTCACTCC+AGG | 0.468606 | 3.1:+83844651 | MS.gene80182:CDS |
| ACGTTCAAGAGGTGGAAGTT+GGG | 0.491157 | 3.1:-83843896 | None:intergenic |
| GCAGCTTGAACAGAAATAGA+TGG | 0.491461 | 3.1:-83844880 | None:intergenic |
| GGGAAAGAAGAGGAAACATC+AGG | 0.491988 | 3.1:+83844816 | MS.gene80182:CDS |
| CTCACAAGCTCTGATTTGAC+AGG | 0.494155 | 3.1:-83844109 | None:intergenic |
| GAAGTTGGGAGAGAGTTTGA+GGG | 0.500143 | 3.1:-83843882 | None:intergenic |
| AGGAGTACCCGAAGCGGTGA+TGG | 0.509427 | 3.1:+83843786 | MS.gene80182:CDS |
| AACCCACCTGGAGTGAGGTC+AGG | 0.514003 | 3.1:-83844657 | None:intergenic |
| AGGAGCAGGCTGGTCAAAAG+AGG | 0.529385 | 3.1:+83844836 | MS.gene80182:CDS |
| AGTGAAAGTGAAAGCTGTGG+AGG | 0.534576 | 3.1:+83843766 | MS.gene80182:CDS |
| GAAGAGGAAACATCAGGAGC+AGG | 0.536755 | 3.1:+83844822 | MS.gene80182:CDS |
| CAGAACAACGGCAGAGAATG+AGG | 0.541135 | 3.1:+83844776 | MS.gene80182:intron |
| AGTGGGGCACGTTCAAGAGG+TGG | 0.562243 | 3.1:-83843904 | None:intergenic |
| GAGAGTGGGGCACGTTCAAG+AGG | 0.566536 | 3.1:-83843907 | None:intergenic |
| GACATGTTGTCAATTTGAAG+TGG | 0.576081 | 3.1:-83844969 | None:intergenic |
| TTCACGAAGATTGGAGAGAG+TGG | 0.578299 | 3.1:-83843821 | None:intergenic |
| GTAATAATGGCGGTATTAAG+GGG | 0.582541 | 3.1:+83844946 | MS.gene80182:CDS |
| AGGAAACATCAGGAGCAGGC+TGG | 0.583685 | 3.1:+83844826 | MS.gene80182:CDS |
| AAAACTGTTGAAAATGTGAA+CGG | 0.603460 | 3.1:-83843846 | None:intergenic |
| GACAATCTTTCAGAAGATGA+CGG | 0.608692 | 3.1:+83845014 | MS.gene80182:CDS |
| AGGAACTTAAGTAGCGGCGA+GGG | 0.610045 | 3.1:+83844796 | MS.gene80182:CDS |
| GCAGTGAAATTGAGGTGCAG+TGG | 0.625638 | 3.1:+83844067 | MS.gene80182:intron |
| GGAAGTGAAAGTGAAAGCTG+TGG | 0.627299 | 3.1:+83843763 | MS.gene80182:CDS |
| AACATAGTAGAAGGTCGCAA+TGG | 0.635548 | 3.1:-83844594 | None:intergenic |
| TTAACAACCCACCTGGAGTG+AGG | 0.652502 | 3.1:-83844662 | None:intergenic |
| GAGGAACTTAAGTAGCGGCG+AGG | 0.667273 | 3.1:+83844795 | MS.gene80182:CDS |
| TCACAAGCTCTGATTTGACA+GGG | 0.674644 | 3.1:-83844108 | None:intergenic |
| CTGTGGAGGAGTACCCGAAG+CGG | 0.685892 | 3.1:+83843780 | MS.gene80182:CDS |
| GTAGCGGCGAGGGAAAGAAG+AGG | 0.690376 | 3.1:+83844806 | MS.gene80182:CDS |
| TTATTTCATATGCAGAACAA+CGG | 0.698855 | 3.1:+83844764 | MS.gene80182:intron |
| AGAATGAGGAACTTAAGTAG+CGG | 0.704922 | 3.1:+83844790 | MS.gene80182:CDS |
| AGTTTCTGGAGAAAGCACAG+AGG | 0.743666 | 3.1:+83844911 | MS.gene80182:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTTCAAAATATAAGATGA+TGG | + | chr3.1:83844337-83844356 | MS.gene80182:intron | 20.0% |
| !! | ATTGAACAAGAAAATTAAGT+TGG | - | chr3.1:83844028-83844047 | None:intergenic | 20.0% |
| ! | AAAACTGTTGAAAATGTGAA+CGG | - | chr3.1:83843849-83843868 | None:intergenic | 25.0% |
| ! | ACAAGTCTAGCTAATAAAAA+GGG | - | chr3.1:83844521-83844540 | None:intergenic | 25.0% |
| ! | ACACTGATGATAATTTAAGA+AGG | + | chr3.1:83844430-83844449 | MS.gene80182:intron | 25.0% |
| ! | TACAAGTCTAGCTAATAAAA+AGG | - | chr3.1:83844522-83844541 | None:intergenic | 25.0% |
| ! | TTATTTCATATGCAGAACAA+CGG | + | chr3.1:83844764-83844783 | MS.gene80182:intron | 25.0% |
| !! | ATGATAATTTAAGAAGGTGT+TGG | + | chr3.1:83844436-83844455 | MS.gene80182:intron | 25.0% |
| !!! | AAGATGATGGATATTTGAAT+TGG | + | chr3.1:83844350-83844369 | MS.gene80182:intron | 25.0% |
| !!! | ATTTTTTTGCAGTGAAATTG+AGG | + | chr3.1:83844059-83844078 | MS.gene80182:intron | 25.0% |
| !!! | GATTTTTGTTGTGTATTTTC+AGG | + | chr3.1:83844188-83844207 | MS.gene80182:intron | 25.0% |
| !!! | TGATAATTTAAGAAGGTGTT+GGG | + | chr3.1:83844437-83844456 | MS.gene80182:intron | 25.0% |
| AACTTGGTTTATTGCTTCTA+GGG | + | chr3.1:83844683-83844702 | MS.gene80182:intron | 30.0% | |
| ACATGTTAACCTTCAATATG+AGG | + | chr3.1:83844474-83844493 | MS.gene80182:intron | 30.0% | |
| ATTAGCTAGACTTGTAGTAT+TGG | + | chr3.1:83844526-83844545 | MS.gene80182:intron | 30.0% | |
| ATTGCATGTATGTAAAGAAG+TGG | + | chr3.1:83844741-83844760 | MS.gene80182:intron | 30.0% | |
| CAAGTCTAGCTAATAAAAAG+GGG | - | chr3.1:83844520-83844539 | None:intergenic | 30.0% | |
| TAACTTGGTTTATTGCTTCT+AGG | + | chr3.1:83844682-83844701 | MS.gene80182:intron | 30.0% | |
| TACATGCAATGTGATCAAAA+TGG | - | chr3.1:83844731-83844750 | None:intergenic | 30.0% | |
| TACCCTTATTCAAATCTAGA+AGG | - | chr3.1:83844248-83844267 | None:intergenic | 30.0% | |
| TGAGAATTCAACATAGTAGA+AGG | - | chr3.1:83844606-83844625 | None:intergenic | 30.0% | |
| ! | TGATTGTGTTTTCGTGATTT+TGG | + | chr3.1:83844555-83844574 | MS.gene80182:intron | 30.0% |
| !! | GAACTTTTTGGTGGTAATAA+TGG | + | chr3.1:83844933-83844952 | MS.gene80182:CDS | 30.0% |
| ACGCCTTCTAGATTTGAATA+AGG | + | chr3.1:83844242-83844261 | MS.gene80182:CDS | 35.0% | |
| AGAATGAGGAACTTAAGTAG+CGG | + | chr3.1:83844790-83844809 | MS.gene80182:CDS | 35.0% | |
| ATCAAACAGAGTAAGCAACA+AGG | - | chr3.1:83844171-83844190 | None:intergenic | 35.0% | |
| CGCCTTCTAGATTTGAATAA+GGG | + | chr3.1:83844243-83844262 | MS.gene80182:CDS | 35.0% | |
| GACAATCTTTCAGAAGATGA+CGG | + | chr3.1:83845014-83845033 | MS.gene80182:CDS | 35.0% | |
| GACATGTTGTCAATTTGAAG+TGG | - | chr3.1:83844972-83844991 | None:intergenic | 35.0% | |
| GGTAATAATGGCGGTATTAA+GGG | + | chr3.1:83844945-83844964 | MS.gene80182:CDS | 35.0% | |
| GTAATAATGGCGGTATTAAG+GGG | + | chr3.1:83844946-83844965 | MS.gene80182:CDS | 35.0% | |
| TAACCTTCAATATGAGGTGT+TGG | + | chr3.1:83844480-83844499 | MS.gene80182:intron | 35.0% | |
| TCAAACAGAGTAAGCAACAA+GGG | - | chr3.1:83844170-83844189 | None:intergenic | 35.0% | |
| TGGTAATAATGGCGGTATTA+AGG | + | chr3.1:83844944-83844963 | MS.gene80182:CDS | 35.0% | |
| !! | CTTTTTGGTGGTAATAATGG+CGG | + | chr3.1:83844936-83844955 | MS.gene80182:CDS | 35.0% |
| AACATAGTAGAAGGTCGCAA+TGG | - | chr3.1:83844597-83844616 | None:intergenic | 40.0% | |
| AGAGTTTGAGGGTTCATAAG+AGG | - | chr3.1:83843874-83843893 | None:intergenic | 40.0% | |
| ATCATCAGTGTCTATGTGTC+AGG | - | chr3.1:83844421-83844440 | None:intergenic | 40.0% | |
| ATGATGATTACGTGTGAGGA+GGG | - | chr3.1:83843960-83843979 | None:intergenic | 40.0% | |
| GAAGATGATGATTACGTGTG+AGG | - | chr3.1:83843964-83843983 | None:intergenic | 40.0% | |
| GATTTGACAGGGTTATCATC+TGG | - | chr3.1:83844100-83844119 | None:intergenic | 40.0% | |
| GCAGCTTGAACAGAAATAGA+TGG | - | chr3.1:83844883-83844902 | None:intergenic | 40.0% | |
| TGAACGGAATTCACGAAGAT+TGG | - | chr3.1:83843833-83843852 | None:intergenic | 40.0% | |
| ! | TCACAAGCTCTGATTTGACA+GGG | - | chr3.1:83844111-83844130 | None:intergenic | 40.0% |
| ! | TTGGTTTATTGCTTCTAGGG+TGG | + | chr3.1:83844686-83844705 | MS.gene80182:intron | 40.0% |
| !! | GAAAGCACAGAGGAACTTTT+TGG | + | chr3.1:83844921-83844940 | MS.gene80182:CDS | 40.0% |
| AACCAAGTTAACAACCCACC+TGG | - | chr3.1:83844672-83844691 | None:intergenic | 45.0% | |
| ACGTTCAAGAGGTGGAAGTT+GGG | - | chr3.1:83843899-83843918 | None:intergenic | 45.0% | |
| AGTGAAAGTGAAAGCTGTGG+AGG | + | chr3.1:83843766-83843785 | MS.gene80182:CDS | 45.0% | |
| CAGAGTAAGCAACAAGGGTT+AGG | - | chr3.1:83844165-83844184 | None:intergenic | 45.0% | |
| GAAGTTGGGAGAGAGTTTGA+GGG | - | chr3.1:83843885-83843904 | None:intergenic | 45.0% | |
| GATGATGATTACGTGTGAGG+AGG | - | chr3.1:83843961-83843980 | None:intergenic | 45.0% | |
| GCACCAACACCTCATATTGA+AGG | - | chr3.1:83844486-83844505 | None:intergenic | 45.0% | |
| GGAAGTGAAAGTGAAAGCTG+TGG | + | chr3.1:83843763-83843782 | MS.gene80182:CDS | 45.0% | |
| GGGAAAGAAGAGGAAACATC+AGG | + | chr3.1:83844816-83844835 | MS.gene80182:CDS | 45.0% | |
| TTCACGAAGATTGGAGAGAG+TGG | - | chr3.1:83843824-83843843 | None:intergenic | 45.0% | |
| ! | AGCACAGAGGAACTTTTTGG+TGG | + | chr3.1:83844924-83844943 | MS.gene80182:CDS | 45.0% |
| ! | AGTTTCTGGAGAAAGCACAG+AGG | + | chr3.1:83844911-83844930 | MS.gene80182:CDS | 45.0% |
| ! | CTCACAAGCTCTGATTTGAC+AGG | - | chr3.1:83844112-83844131 | None:intergenic | 45.0% |
| ! | GAGGAGGGTAGAAGTGATTT+TGG | - | chr3.1:83843945-83843964 | None:intergenic | 45.0% |
| !! | GGGTAGAAGTGATTTTGGAG+AGG | - | chr3.1:83843940-83843959 | None:intergenic | 45.0% |
| ACTGAATCCATCACCGCTTC+GGG | - | chr3.1:83843796-83843815 | None:intergenic | 50.0% | |
| AGGAACTTAAGTAGCGGCGA+GGG | + | chr3.1:83844796-83844815 | MS.gene80182:CDS | 50.0% | |
| CACGTTCAAGAGGTGGAAGT+TGG | - | chr3.1:83843900-83843919 | None:intergenic | 50.0% | |
| CAGAACAACGGCAGAGAATG+AGG | + | chr3.1:83844776-83844795 | MS.gene80182:intron | 50.0% | |
| GAAGAGGAAACATCAGGAGC+AGG | + | chr3.1:83844822-83844841 | MS.gene80182:CDS | 50.0% | |
| GACTGAATCCATCACCGCTT+CGG | - | chr3.1:83843797-83843816 | None:intergenic | 50.0% | |
| GAGAGGAGAAAGAGAGAGTG+GGG | - | chr3.1:83843923-83843942 | None:intergenic | 50.0% | |
| GCAGTGAAATTGAGGTGCAG+TGG | + | chr3.1:83844067-83844086 | MS.gene80182:intron | 50.0% | |
| GGAAGTTGGGAGAGAGTTTG+AGG | - | chr3.1:83843886-83843905 | None:intergenic | 50.0% | |
| GGAGAGGAGAAAGAGAGAGT+GGG | - | chr3.1:83843924-83843943 | None:intergenic | 50.0% | |
| TGGAGAGGAGAAAGAGAGAG+TGG | - | chr3.1:83843925-83843944 | None:intergenic | 50.0% | |
| TTAACAACCCACCTGGAGTG+AGG | - | chr3.1:83844665-83844684 | None:intergenic | 50.0% | |
| TTCTGTTCAAGCTGCTGCTC+AGG | + | chr3.1:83844888-83844907 | MS.gene80182:CDS | 50.0% | |
| !! | CTCCAGGTGGGTTGTTAACT+TGG | + | chr3.1:83844667-83844686 | MS.gene80182:intron | 50.0% |
| AGCTGCTGCTCAGGAGTTTC+TGG | + | chr3.1:83844897-83844916 | MS.gene80182:CDS | 55.0% | |
| AGGAAACATCAGGAGCAGGC+TGG | + | chr3.1:83844826-83844845 | MS.gene80182:CDS | 55.0% | |
| GAGGAACTTAAGTAGCGGCG+AGG | + | chr3.1:83844795-83844814 | MS.gene80182:CDS | 55.0% | |
| TCTTTGCCTGACCTCACTCC+AGG | + | chr3.1:83844651-83844670 | MS.gene80182:CDS | 55.0% | |
| ! | AGGAGCAGGCTGGTCAAAAG+AGG | + | chr3.1:83844836-83844855 | MS.gene80182:CDS | 55.0% |
| AACCCACCTGGAGTGAGGTC+AGG | - | chr3.1:83844660-83844679 | None:intergenic | 60.0% | |
| AGTGGGGCACGTTCAAGAGG+TGG | - | chr3.1:83843907-83843926 | None:intergenic | 60.0% | |
| GAGAGTGGGGCACGTTCAAG+AGG | - | chr3.1:83843910-83843929 | None:intergenic | 60.0% | |
| GTAGCGGCGAGGGAAAGAAG+AGG | + | chr3.1:83844806-83844825 | MS.gene80182:CDS | 60.0% | |
| TGCCTGACCTCACTCCAGGT+GGG | + | chr3.1:83844655-83844674 | MS.gene80182:intron | 60.0% | |
| TTGCCTGACCTCACTCCAGG+TGG | + | chr3.1:83844654-83844673 | MS.gene80182:intron | 60.0% | |
| !! | AGGAGTACCCGAAGCGGTGA+TGG | + | chr3.1:83843786-83843805 | MS.gene80182:CDS | 60.0% |
| !! | CTGTGGAGGAGTACCCGAAG+CGG | + | chr3.1:83843780-83843799 | MS.gene80182:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 83843754 | 83845053 | 83843754 | ID=MS.gene80182 |
| chr3.1 | mRNA | 83843754 | 83845053 | 83843754 | ID=MS.gene80182.t1;Parent=MS.gene80182 |
| chr3.1 | exon | 83843754 | 83843916 | 83843754 | ID=MS.gene80182.t1.exon1;Parent=MS.gene80182.t1 |
| chr3.1 | CDS | 83843754 | 83843916 | 83843754 | ID=cds.MS.gene80182.t1;Parent=MS.gene80182.t1 |
| chr3.1 | exon | 83844071 | 83844126 | 83844071 | ID=MS.gene80182.t1.exon2;Parent=MS.gene80182.t1 |
| chr3.1 | CDS | 83844071 | 83844126 | 83844071 | ID=cds.MS.gene80182.t1;Parent=MS.gene80182.t1 |
| chr3.1 | exon | 83844210 | 83844264 | 83844210 | ID=MS.gene80182.t1.exon3;Parent=MS.gene80182.t1 |
| chr3.1 | CDS | 83844210 | 83844264 | 83844210 | ID=cds.MS.gene80182.t1;Parent=MS.gene80182.t1 |
| chr3.1 | exon | 83844592 | 83844672 | 83844592 | ID=MS.gene80182.t1.exon4;Parent=MS.gene80182.t1 |
| chr3.1 | CDS | 83844592 | 83844672 | 83844592 | ID=cds.MS.gene80182.t1;Parent=MS.gene80182.t1 |
| chr3.1 | exon | 83844779 | 83845053 | 83844779 | ID=MS.gene80182.t1.exon5;Parent=MS.gene80182.t1 |
| chr3.1 | CDS | 83844779 | 83845053 | 83844779 | ID=cds.MS.gene80182.t1;Parent=MS.gene80182.t1 |
| Gene Sequence |
| Protein sequence |
