AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82993

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82993.t1 MTR_1g033420 96.646 328 11 0 1 328 1 328 0.0 670
MS.gene82993.t1 MTR_5g029100 57.292 288 118 2 27 309 36 323 3.65e-120 350
MS.gene82993.t1 MTR_5g029100 55.596 277 106 3 38 309 28 292 5.44e-107 315
MS.gene82993.t1 MTR_8g090100 50.000 286 131 5 21 299 9 289 2.10e-96 288
MS.gene82993.t1 MTR_8g064180 46.324 272 140 1 26 291 39 310 4.03e-85 259
MS.gene82993.t1 MTR_4g057450 43.478 276 141 5 19 291 41 304 7.83e-72 225
MS.gene82993.t1 MTR_2g095800 40.468 299 157 7 7 291 18 309 5.03e-67 213
MS.gene82993.t1 MTR_1g063020 39.394 297 159 7 8 291 6 294 1.42e-65 209
MS.gene82993.t1 MTR_7g102260 40.068 292 160 6 7 291 8 291 4.13e-64 205
MS.gene82993.t1 MTR_8g013055 40.520 269 142 6 31 292 29 286 9.48e-63 201
MS.gene82993.t1 MTR_6g033085 37.584 298 167 9 4 294 5 290 1.44e-59 193
MS.gene82993.t1 MTR_7g084750 37.770 278 160 6 22 294 21 290 1.96e-58 190
MS.gene82993.t1 MTR_6g033055 36.644 292 167 7 12 294 8 290 3.53e-57 187
MS.gene82993.t1 MTR_7g084760 36.395 294 174 7 8 295 5 291 1.49e-56 185
MS.gene82993.t1 MTR_4g097640 41.975 243 134 6 52 292 51 288 1.58e-56 185
MS.gene82993.t1 MTR_4g097670 41.975 243 134 6 52 292 51 288 1.58e-56 185
MS.gene82993.t1 MTR_4g097700 39.405 269 154 7 28 292 62 325 2.36e-56 186
MS.gene82993.t1 MTR_4g045853 37.037 297 164 8 7 292 5 289 2.98e-56 184
MS.gene82993.t1 MTR_7g084770 36.897 290 170 6 11 294 8 290 5.46e-56 184
MS.gene82993.t1 MTR_2g089140 36.755 302 166 8 1 292 1 287 5.80e-56 184
MS.gene82993.t1 MTR_5g091730 37.193 285 158 8 14 291 8 278 2.54e-55 182
MS.gene82993.t1 MTR_1g105495 34.892 278 148 4 33 293 35 296 1.94e-53 177
MS.gene82993.t1 MTR_4g128410 40.310 258 137 8 41 291 33 280 3.02e-53 176
MS.gene82993.t1 MTR_1g105900 36.842 266 157 7 33 293 34 293 3.46e-53 177
MS.gene82993.t1 MTR_4g126920 36.981 265 157 6 33 293 25 283 6.03e-53 176
MS.gene82993.t1 MTR_7g056090 35.034 294 170 8 7 293 4 283 1.53e-51 172
MS.gene82993.t1 MTR_7g093530 37.500 256 151 5 41 293 32 281 3.92e-51 171
MS.gene82993.t1 MTR_4g128400 37.500 256 151 5 41 293 36 285 6.83e-51 171
MS.gene82993.t1 MTR_4g128570 35.154 293 157 8 4 292 10 273 7.60e-51 170
MS.gene82993.t1 MTR_2g049130 33.818 275 167 8 7 278 6 268 1.92e-50 169
MS.gene82993.t1 MTR_2g049030 33.447 293 171 10 6 291 1 276 1.06e-49 167
MS.gene82993.t1 MTR_3g089540 36.466 266 159 6 33 296 30 287 2.50e-49 166
MS.gene82993.t1 MTR_2g089140 39.301 229 124 6 74 292 1 224 2.72e-49 164
MS.gene82993.t1 MTR_4g128580 37.828 267 153 7 33 292 26 286 3.70e-49 166
MS.gene82993.t1 MTR_3g084630 37.165 261 151 6 33 291 28 277 1.72e-48 164
MS.gene82993.t1 MTR_6g088320 32.993 294 168 10 10 291 7 283 1.74e-48 164
MS.gene82993.t1 MTR_3g084620 37.165 261 151 6 33 291 28 277 2.19e-48 164
MS.gene82993.t1 MTR_2g038860 36.398 261 150 6 41 292 34 287 1.30e-47 162
MS.gene82993.t1 MTR_2g038705 36.398 261 150 6 41 292 34 287 1.30e-47 162
MS.gene82993.t1 MTR_8g102910 35.632 261 154 7 37 291 27 279 4.94e-46 158
MS.gene82993.t1 MTR_8g102220 37.165 261 139 8 33 291 29 266 9.57e-46 157
MS.gene82993.t1 MTR_8g081040 35.632 261 156 5 33 291 31 281 2.25e-45 156
MS.gene82993.t1 MTR_4g128590 36.239 218 125 6 82 291 64 275 6.02e-40 142
MS.gene82993.t1 MTR_8g032640 36.788 193 108 6 37 228 19 198 2.20e-21 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82993.t1 AT1G14720 66.032 315 105 2 9 321 7 321 1.17e-158 447
MS.gene82993.t1 AT2G01850 63.190 326 116 3 1 324 1 324 1.89e-155 439
MS.gene82993.t1 AT1G32170 55.484 310 129 4 5 310 8 312 1.13e-121 353
MS.gene82993.t1 AT4G18990 53.618 304 126 3 27 316 34 336 1.58e-110 325
MS.gene82993.t1 AT1G10550 44.056 286 148 2 16 291 26 309 9.26e-82 251
MS.gene82993.t1 AT4G18990 52.586 232 95 3 99 316 32 262 1.99e-81 249
MS.gene82993.t1 AT4G13090 42.857 294 154 6 6 291 1 288 8.69e-72 224
MS.gene82993.t1 AT4G13080 39.661 295 167 5 1 291 4 291 8.64e-69 217
MS.gene82993.t1 AT3G25050 38.144 291 164 6 10 291 6 289 1.29e-64 206
MS.gene82993.t1 AT5G13870 37.584 298 174 6 1 294 1 290 4.57e-64 204
MS.gene82993.t1 AT5G13870 37.584 298 174 6 1 294 1 290 4.57e-64 204
MS.gene82993.t1 AT5G13870 37.584 298 174 6 1 294 1 290 4.57e-64 204
MS.gene82993.t1 AT3G44990 40.217 276 154 5 19 291 26 293 7.42e-64 204
MS.gene82993.t1 AT2G06850 40.476 294 159 8 6 294 11 293 1.09e-62 201
MS.gene82993.t1 AT2G36870 38.667 300 163 6 6 291 5 297 2.58e-62 200
MS.gene82993.t1 AT5G65730 40.278 288 158 7 8 291 13 290 7.83e-62 199
MS.gene82993.t1 AT4G37800 40.304 263 146 6 33 291 34 289 6.58e-61 196
MS.gene82993.t1 AT4G03210 40.152 264 145 6 33 291 29 284 2.47e-58 190
MS.gene82993.t1 AT1G11545 35.667 300 168 7 7 293 14 301 3.25e-56 185
MS.gene82993.t1 AT4G03210 41.126 231 127 4 65 291 31 256 4.06e-55 181
MS.gene82993.t1 AT2G06850 42.797 236 124 5 63 294 5 233 4.80e-55 179
MS.gene82993.t1 AT4G25810 38.996 259 143 8 41 292 34 284 2.36e-52 174
MS.gene82993.t1 AT5G57560 35.959 292 169 7 7 292 3 282 5.93e-52 173
MS.gene82993.t1 AT3G23730 36.604 265 157 5 33 291 27 286 5.88e-50 168
MS.gene82993.t1 AT5G57550 37.023 262 153 7 33 291 30 282 6.16e-50 168
MS.gene82993.t1 AT4G30270 35.294 289 159 7 10 295 6 269 1.24e-49 167
MS.gene82993.t1 AT4G14130 37.023 262 156 6 33 291 29 284 1.56e-48 164
MS.gene82993.t1 AT2G18800 35.664 286 156 7 33 299 28 304 1.62e-48 165
MS.gene82993.t1 AT5G48070 37.500 248 142 7 47 291 44 281 5.01e-48 163
MS.gene82993.t1 AT4G28850 33.333 291 169 9 14 291 9 287 1.09e-47 162
MS.gene82993.t1 AT2G14620 36.255 251 145 8 50 293 54 296 1.23e-47 162
MS.gene82993.t1 AT5G57530 37.405 262 145 9 41 294 35 285 2.63e-47 161
MS.gene82993.t1 AT4G30290 39.655 232 125 7 64 291 56 276 8.23e-47 159
MS.gene82993.t1 AT4G30280 38.528 231 129 6 64 291 61 281 9.31e-47 159
MS.gene82993.t1 AT5G57540 35.338 266 161 7 33 294 26 284 3.49e-46 158
MS.gene82993.t1 AT1G65310 38.281 256 144 7 41 292 37 282 1.72e-45 156
MS.gene82993.t1 AT1G65310 38.281 256 144 7 41 292 59 304 2.68e-45 156
MS.gene82993.t1 AT2G14620 36.287 237 136 8 64 293 18 246 1.53e-44 153
MS.gene82993.t1 AT2G14620 36.404 228 130 8 73 293 16 235 1.35e-42 147
MS.gene82993.t1 AT4G25820 34.091 264 165 5 33 293 30 287 1.25e-41 146
MS.gene82993.t1 AT3G48580 32.700 263 154 11 31 291 32 273 3.60e-35 129
MS.gene82993.t1 AT2G36870 40.909 176 88 4 6 167 5 178 9.62e-33 120
MS.gene82993.t1 AT3G48580 33.333 231 132 10 63 291 4 214 5.58e-31 116
MS.gene82993.t1 AT3G48580 29.658 263 142 12 31 291 32 253 4.33e-22 94.0

Find 63 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGATTCAAACAAATGTTTA+TGG 0.147337 1.4:+18203272 MS.gene82993:CDS
GAGAAGAAAGATATGGTTTA+TGG 0.194534 1.4:+18203318 MS.gene82993:CDS
TTCATCAGTATAGTATTCTT+TGG 0.249093 1.4:+18203360 MS.gene82993:CDS
TCTCATGATCTTTACCTTCA+TGG 0.260057 1.4:+18202336 MS.gene82993:CDS
TACAAAGTCATTGCCTTTGA+TGG 0.283691 1.4:-18204389 None:intergenic
CTCTTACTTGATGAGTCTTC+AGG 0.286306 1.4:+18202207 MS.gene82993:CDS
CAAACAAATGTTTATGGAAA+TGG 0.295488 1.4:+18203278 MS.gene82993:CDS
GAATCAAAATGGATAACTTT+AGG 0.296907 1.4:+18204609 MS.gene82993:CDS
CCTTCATCAAAAGGTATAAT+AGG 0.299209 1.4:-18202123 None:intergenic
AAAACGAAGTAACTTTGTTT+TGG 0.306141 1.4:-18204787 None:intergenic
ATAATCAGAAGGTAATTTAA+TGG 0.316688 1.4:-18202373 None:intergenic
ACAAGTGGAAAGAATCAAAA+TGG 0.328261 1.4:+18204598 MS.gene82993:CDS
GTCAGAGAATTCAGTAATAT+AGG 0.334740 1.4:-18204483 None:intergenic
CGGTAGAGGGACTTTGTATC+TGG 0.344053 1.4:-18204669 None:intergenic
GAAGGGTATACACCTTTGTT+TGG 0.357285 1.4:+18202141 MS.gene82993:CDS
TGACTTTGTATTCTACCATA+TGG 0.360868 1.4:+18204402 MS.gene82993:CDS
GGTATAATAGGAAAATTTCT+TGG 0.364767 1.4:-18202111 None:intergenic
CCATATGGGATGCATCTACT+TGG 0.374925 1.4:+18204417 MS.gene82993:CDS
GTTCTCTTTACTTCTCTGAT+AGG 0.375696 1.4:-18204344 None:intergenic
AAATTTGATCCCGTTACATT+TGG 0.384702 1.4:+18204731 MS.gene82993:CDS
GTCTCTTTCCACGGTGGTGC+CGG 0.385909 1.4:-18204760 None:intergenic
CACTTGGTCACATGCTCAAT+AGG 0.402506 1.4:-18204533 None:intergenic
TTCTCTGACTTTGTCCTACA+TGG 0.411675 1.4:+18204497 MS.gene82993:CDS
TCAGAGAATTCAGTAATATA+GGG 0.425050 1.4:-18204482 None:intergenic
ATTGGAAGAGAAGAAAGATA+TGG 0.430520 1.4:+18203311 MS.gene82993:CDS
TTACCTTCTGATTATACTGC+TGG 0.434184 1.4:+18202381 MS.gene82993:CDS
GACTTTGTATTCTACCATAT+GGG 0.438803 1.4:+18204403 MS.gene82993:CDS
GATCCCGTTACATTTGGTAA+TGG 0.449715 1.4:+18204737 MS.gene82993:CDS
TTTATTCTGCAGTTGTCAAA+TGG 0.450372 1.4:+18203179 MS.gene82993:intron
CAGAATGAATGATCAAGTTA+TGG 0.454056 1.4:-18202167 None:intergenic
GGTGTATACCCTTCATCAAA+AGG 0.456262 1.4:-18202132 None:intergenic
GGAACATAAGAGGCAAAGAT+TGG 0.477793 1.4:+18203249 MS.gene82993:CDS
CCAAGTAGATGCATCCCATA+TGG 0.478573 1.4:-18204417 None:intergenic
AAGTAAGAGATGAACAGCTT+TGG 0.482296 1.4:-18202193 None:intergenic
TCGATCACACACTCCGGTAG+AGG 0.485145 1.4:-18204683 None:intergenic
TCCTTCTGATGTTTCACAAG+TGG 0.485339 1.4:+18204583 MS.gene82993:CDS
ATTCAAAGTCTATTTCATCA+TGG 0.490439 1.4:-18203220 None:intergenic
CCGTTACATTTGGTAATGGC+CGG 0.501258 1.4:+18204741 MS.gene82993:CDS
CGGCCATTACCAAATGTAAC+GGG 0.507577 1.4:-18204740 None:intergenic
TGATTATACTGCTGGAGTTG+TGG 0.518642 1.4:+18202389 MS.gene82993:CDS
TTTGGCGGTGTCTCTTTCCA+CGG 0.520645 1.4:-18204769 None:intergenic
ACTTTGAGCACTGTCACACT+TGG 0.532982 1.4:-18204549 None:intergenic
GGAAATGGAAGTACAAACAT+TGG 0.549278 1.4:+18203293 MS.gene82993:CDS
AAGCACTAAAATAGCCATGA+AGG 0.555543 1.4:-18202350 None:intergenic
GCATCTACTTGGGCTACCAA+TGG 0.559060 1.4:+18204428 MS.gene82993:CDS
TCGATTCGCGACAAGCTGAA+AGG 0.568686 1.4:+18204702 MS.gene82993:CDS
TACTGATGAAATTCCTCAGA+AGG 0.576854 1.4:-18203347 None:intergenic
TCCACTTGTGAAACATCAGA+AGG 0.579841 1.4:-18204584 None:intergenic
CCGGCCATTACCAAATGTAA+CGG 0.580145 1.4:-18204741 None:intergenic
CGATCACACACTCCGGTAGA+GGG 0.581239 1.4:-18204682 None:intergenic
ACATAAGAGGCAAAGATTGG+AGG 0.581414 1.4:+18203252 MS.gene82993:CDS
CAGATACAAAGTCCCTCTAC+CGG 0.595007 1.4:+18204670 MS.gene82993:CDS
CTTGGTGAAACAGAGTAACA+AGG 0.597670 1.4:-18202093 None:intergenic
TCTACTTGGGCTACCAATGG+AGG 0.600953 1.4:+18204431 MS.gene82993:CDS
TTTATGGTTTGATCCTTCTG+AGG 0.601961 1.4:+18203334 MS.gene82993:CDS
CATATGGGATGCATCTACTT+GGG 0.612597 1.4:+18204418 MS.gene82993:CDS
GATCAACTGCACAACCATGT+AGG 0.614567 1.4:-18204511 None:intergenic
AAGAGAACTGAGTCAATGAG+AGG 0.626190 1.4:+18204359 MS.gene82993:CDS
ACTCCAGCAGTATAATCAGA+AGG 0.629305 1.4:-18202384 None:intergenic
GGCGGTGTCTCTTTCCACGG+TGG 0.643693 1.4:-18204766 None:intergenic
AAGTTATGGTCTCCAAACAA+AGG 0.651449 1.4:-18202153 None:intergenic
CGCGAATCGATCACACACTC+CGG 0.652371 1.4:-18204689 None:intergenic
GGTAATGGCCGGCACCACCG+TGG 0.687743 1.4:+18204752 MS.gene82993:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAATTAAAATATTAAAAA+TGG - chr1.4:18202772-18202791 None:intergenic 0.0%
!! AACTAAATATAGCATATTAA+GGG - chr1.4:18203424-18203443 None:intergenic 15.0%
!! AATTTAATGATTGAAAAACA+CGG + chr1.4:18204083-18204102 MS.gene82993:intron 15.0%
!! TAACTAAATATAGCATATTA+AGG - chr1.4:18203425-18203444 None:intergenic 15.0%
!! TATGTATATATATCCTTAAA+GGG + chr1.4:18203705-18203724 MS.gene82993:intron 15.0%
!! TTATGTATATATATCCTTAA+AGG + chr1.4:18203704-18203723 MS.gene82993:intron 15.0%
!! ACATAATTCACTATAACATT+TGG - chr1.4:18202515-18202534 None:intergenic 20.0%
!! ATAATCAGAAGGTAATTTAA+TGG - chr1.4:18202376-18202395 None:intergenic 20.0%
!! ATGTATATATATCCTTAAAG+GGG + chr1.4:18203706-18203725 MS.gene82993:intron 20.0%
!! GATTGCTAATTCATAAATTT+AGG - chr1.4:18203836-18203855 None:intergenic 20.0%
!! TAGTCTATAAAACACTAATA+TGG + chr1.4:18202596-18202615 MS.gene82993:intron 20.0%
!! TTAAAATACAACTCAAAACA+CGG - chr1.4:18204059-18204078 None:intergenic 20.0%
!! TTCTGATACATATTTCTTAT+AGG - chr1.4:18203990-18204009 None:intergenic 20.0%
!! TTCTTGATTAACTTTCATTA+TGG + chr1.4:18204216-18204235 MS.gene82993:intron 20.0%
!!! AAATAGACTTTGAATTTTTG+GGG + chr1.4:18203228-18203247 MS.gene82993:CDS 20.0%
!!! ATGTTAATGGTTAATTTGTT+AGG - chr1.4:18203964-18203983 None:intergenic 20.0%
!!! ATTTCTTTCTTCCATTTTTT+AGG - chr1.4:18203140-18203159 None:intergenic 20.0%
!!! CACTAATTTTACCTAAAAAA+TGG + chr1.4:18203126-18203145 MS.gene82993:intron 20.0%
!!! GAAATAGACTTTGAATTTTT+GGG + chr1.4:18203227-18203246 MS.gene82993:CDS 20.0%
!!! GTGTTTTTCAATCATTAAAT+TGG - chr1.4:18204084-18204103 None:intergenic 20.0%
!!! TGAAATAGACTTTGAATTTT+TGG + chr1.4:18203226-18203245 MS.gene82993:CDS 20.0%
! AGGATTCAAACAAATGTTTA+TGG + chr1.4:18203272-18203291 MS.gene82993:CDS 25.0%
! ATATACATTTGAGAGATAGA+TGG + chr1.4:18204187-18204206 MS.gene82993:intron 25.0%
! ATTCAAAGTCTATTTCATCA+TGG - chr1.4:18203223-18203242 None:intergenic 25.0%
! CAAACAAATGTTTATGGAAA+TGG + chr1.4:18203278-18203297 MS.gene82993:CDS 25.0%
! GAATCAAAATGGATAACTTT+AGG + chr1.4:18204609-18204628 MS.gene82993:CDS 25.0%
! GATGTTAATAATGAGGTTTA+GGG + chr1.4:18203932-18203951 MS.gene82993:intron 25.0%
! GGTATAATAGGAAAATTTCT+TGG - chr1.4:18202114-18202133 None:intergenic 25.0%
! TAGATCATTAGTATATGATG+AGG - chr1.4:18202436-18202455 None:intergenic 25.0%
! TCAGAGAATTCAGTAATATA+GGG - chr1.4:18204485-18204504 None:intergenic 25.0%
! TTAGAAATTCAACGTAATCA+TGG - chr1.4:18203043-18203062 None:intergenic 25.0%
! TTATAAAGATTCCAATACCT+AGG - chr1.4:18203782-18203801 None:intergenic 25.0%
! TTCATCAGTATAGTATTCTT+TGG + chr1.4:18203360-18203379 MS.gene82993:CDS 25.0%
! TTCTTATAGGCAAATGTTAA+TGG - chr1.4:18203977-18203996 None:intergenic 25.0%
!! ATGTACAATTTTTGCATTGA+TGG + chr1.4:18203630-18203649 MS.gene82993:intron 25.0%
!! ATTCTTTTGCAGATTTTATG+TGG + chr1.4:18204313-18204332 MS.gene82993:intron 25.0%
!! CTATTATACCTTTTGATGAA+GGG + chr1.4:18202124-18202143 MS.gene82993:CDS 25.0%
!! CTTTAGAAAATGATTTGAAC+CGG + chr1.4:18202649-18202668 MS.gene82993:intron 25.0%
!! TATAAAGTTCTGATGACATA+TGG - chr1.4:18203677-18203696 None:intergenic 25.0%
!! TTAGTCTTTATGATCAGTAA+AGG + chr1.4:18202836-18202855 MS.gene82993:intron 25.0%
!!! AAAACGAAGTAACTTTGTTT+TGG - chr1.4:18204790-18204809 None:intergenic 25.0%
!!! TTTTGAAAGAAACTGTCATT+TGG + chr1.4:18202936-18202955 MS.gene82993:intron 25.0%
AAATTTGATCCCGTTACATT+TGG + chr1.4:18204731-18204750 MS.gene82993:CDS 30.0%
AACCCACAAGATAAAAAAGT+TGG + chr1.4:18203732-18203751 MS.gene82993:intron 30.0%
ACAAGTGGAAAGAATCAAAA+TGG + chr1.4:18204598-18204617 MS.gene82993:CDS 30.0%
ATACCTAGGAATTTATCCAA+TGG - chr1.4:18203768-18203787 None:intergenic 30.0%
ATATGTATCAGAAGTAACTG+CGG + chr1.4:18203997-18204016 MS.gene82993:intron 30.0%
ATGAACGGATGTTAATAATG+AGG + chr1.4:18203925-18203944 MS.gene82993:intron 30.0%
ATTGGAAGAGAAGAAAGATA+TGG + chr1.4:18203311-18203330 MS.gene82993:CDS 30.0%
CAGAATGAATGATCAAGTTA+TGG - chr1.4:18202170-18202189 None:intergenic 30.0%
GACTTTGTATTCTACCATAT+GGG + chr1.4:18204403-18204422 MS.gene82993:CDS 30.0%
GAGAAGAAAGATATGGTTTA+TGG + chr1.4:18203318-18203337 MS.gene82993:CDS 30.0%
GGATGTTAATAATGAGGTTT+AGG + chr1.4:18203931-18203950 MS.gene82993:intron 30.0%
GGTAAAATTAGTGCAATTGA+TGG - chr1.4:18203119-18203138 None:intergenic 30.0%
GTCAGAGAATTCAGTAATAT+AGG - chr1.4:18204486-18204505 None:intergenic 30.0%
TATATATATCCTTAAAGGGG+AGG + chr1.4:18203709-18203728 MS.gene82993:intron 30.0%
TGACTTTGTATTCTACCATA+TGG + chr1.4:18204402-18204421 MS.gene82993:CDS 30.0%
TTGGATAAATTCCTAGGTAT+TGG + chr1.4:18203768-18203787 MS.gene82993:intron 30.0%
TTTATTCTGCAGTTGTCAAA+TGG + chr1.4:18203179-18203198 MS.gene82993:intron 30.0%
! AATATCAAACAGACTTTTGC+TGG - chr1.4:18202917-18202936 None:intergenic 30.0%
! CCTATTATACCTTTTGATGA+AGG + chr1.4:18202123-18202142 MS.gene82993:CDS 30.0%
! CCTTCATCAAAAGGTATAAT+AGG - chr1.4:18202126-18202145 None:intergenic 30.0%
! CGGTTCAAATCATTTTCTAA+AGG - chr1.4:18202651-18202670 None:intergenic 30.0%
! TACTTTGTATTTTCCTCCAT+TGG - chr1.4:18204447-18204466 None:intergenic 30.0%
! TGAACAAAAAGTACAAGCAA+GGG - chr1.4:18203524-18203543 None:intergenic 30.0%
! TTGAACAAAAAGTACAAGCA+AGG - chr1.4:18203525-18203544 None:intergenic 30.0%
!! CAAATCATTTTCTAAAGGTG+TGG - chr1.4:18202646-18202665 None:intergenic 30.0%
!! TTGTGTTTTGTTCTGTTTTC+AGG + chr1.4:18202300-18202319 MS.gene82993:intron 30.0%
!!! AACTGGTTTTTGCTTTTAGT+AGG + chr1.4:18203598-18203617 MS.gene82993:intron 30.0%
!!! CACCAACTTTTTTATCTTGT+GGG - chr1.4:18203737-18203756 None:intergenic 30.0%
AAAGACGTATGAAACCTACT+TGG - chr1.4:18203878-18203897 None:intergenic 35.0%
AAAGATTGACAAAATCGCGA+TGG + chr1.4:18203083-18203102 MS.gene82993:intron 35.0%
AAATCTGTGGATGTGGTAAA+TGG - chr1.4:18203573-18203592 None:intergenic 35.0%
AAGCACTAAAATAGCCATGA+AGG - chr1.4:18202353-18202372 None:intergenic 35.0%
AAGTAAGAGATGAACAGCTT+TGG - chr1.4:18202196-18202215 None:intergenic 35.0%
AAGTTATGGTCTCCAAACAA+AGG - chr1.4:18202156-18202175 None:intergenic 35.0%
ATCCACAGATTTGTGAAAAC+TGG + chr1.4:18203581-18203600 MS.gene82993:intron 35.0%
CCACAAGATAAAAAAGTTGG+TGG + chr1.4:18203735-18203754 MS.gene82993:intron 35.0%
GTTCTCTTTACTTCTCTGAT+AGG - chr1.4:18204347-18204366 None:intergenic 35.0%
TACAAAGTCATTGCCTTTGA+TGG - chr1.4:18204392-18204411 None:intergenic 35.0%
TACTGATGAAATTCCTCAGA+AGG - chr1.4:18203350-18203369 None:intergenic 35.0%
TCTCATGATCTTTACCTTCA+TGG + chr1.4:18202336-18202355 MS.gene82993:CDS 35.0%
TGACCATTGGATAAATTCCT+AGG + chr1.4:18203762-18203781 MS.gene82993:intron 35.0%
TGGATGTGGTAAATGGTAAA+AGG - chr1.4:18203566-18203585 None:intergenic 35.0%
TTACCTTCTGATTATACTGC+TGG + chr1.4:18202381-18202400 MS.gene82993:CDS 35.0%
TTGTTCCTTAGTTACCAAGT+AGG + chr1.4:18203861-18203880 MS.gene82993:intron 35.0%
TTTATGGTTTGATCCTTCTG+AGG + chr1.4:18203334-18203353 MS.gene82993:CDS 35.0%
! AAAAAGTACAAGCAAGGGAA+TGG - chr1.4:18203519-18203538 None:intergenic 35.0%
! AACCAGTTTTCACAAATCTG+TGG - chr1.4:18203586-18203605 None:intergenic 35.0%
! ACAGACTTTTGCTGGAAAAA+AGG - chr1.4:18202909-18202928 None:intergenic 35.0%
! CAGACTTTTGCTGGAAAAAA+GGG - chr1.4:18202908-18202927 None:intergenic 35.0%
! GGAAATGGAAGTACAAACAT+TGG + chr1.4:18203293-18203312 MS.gene82993:CDS 35.0%
! TGAAACCTACTTGGTAACTA+AGG - chr1.4:18203869-18203888 None:intergenic 35.0%
! TTTTCACAAATCTGTGGATG+TGG - chr1.4:18203580-18203599 None:intergenic 35.0%
!! GAATTTTTGGGGAACATAAG+AGG + chr1.4:18203239-18203258 MS.gene82993:CDS 35.0%
!!! ACGAAGTAACTTTGTTTTGG+CGG - chr1.4:18204787-18204806 None:intergenic 35.0%
!!! CCACCAACTTTTTTATCTTG+TGG - chr1.4:18203738-18203757 None:intergenic 35.0%
AAATGATTTGAACCGGTCGT+TGG + chr1.4:18202656-18202675 MS.gene82993:intron 40.0%
AAGAGAACTGAGTCAATGAG+AGG + chr1.4:18204359-18204378 MS.gene82993:CDS 40.0%
ACATAAGAGGCAAAGATTGG+AGG + chr1.4:18203252-18203271 MS.gene82993:CDS 40.0%
ACCATTGGATGAAGATCAGA+CGG - chr1.4:18204110-18204129 None:intergenic 40.0%
ACTCCAGCAGTATAATCAGA+AGG - chr1.4:18202387-18202406 None:intergenic 40.0%
ATCGTCTCAACTCTTACAAC+AGG - chr1.4:18203907-18203926 None:intergenic 40.0%
CATATGGGATGCATCTACTT+GGG + chr1.4:18204418-18204437 MS.gene82993:CDS 40.0%
CTCTTACTTGATGAGTCTTC+AGG + chr1.4:18202207-18202226 MS.gene82993:CDS 40.0%
CTTGGTGAAACAGAGTAACA+AGG - chr1.4:18202096-18202115 None:intergenic 40.0%
GATCCCGTTACATTTGGTAA+TGG + chr1.4:18204737-18204756 MS.gene82993:CDS 40.0%
GGAACATAAGAGGCAAAGAT+TGG + chr1.4:18203249-18203268 MS.gene82993:CDS 40.0%
GGTGTATACCCTTCATCAAA+AGG - chr1.4:18202135-18202154 None:intergenic 40.0%
GTAAGAGTTGAGACGATGAA+CGG + chr1.4:18203910-18203929 MS.gene82993:intron 40.0%
TAGACTATGAAACAGAGCCA+TGG - chr1.4:18202583-18202602 None:intergenic 40.0%
TAGTTGCAGATGTCAACCAT+TGG - chr1.4:18204125-18204144 None:intergenic 40.0%
TATGAAACACAGACACTCCT+CGG + chr1.4:18202461-18202480 MS.gene82993:intron 40.0%
TCAACTCTTACAACAGGGTT+CGG - chr1.4:18203901-18203920 None:intergenic 40.0%
TCCACTTGTGAAACATCAGA+AGG - chr1.4:18204587-18204606 None:intergenic 40.0%
TCCTTCTGATGTTTCACAAG+TGG + chr1.4:18204583-18204602 MS.gene82993:CDS 40.0%
TCGTCTCAACTCTTACAACA+GGG - chr1.4:18203906-18203925 None:intergenic 40.0%
TGATTATACTGCTGGAGTTG+TGG + chr1.4:18202389-18202408 MS.gene82993:CDS 40.0%
TTCTCTGACTTTGTCCTACA+TGG + chr1.4:18204497-18204516 MS.gene82993:CDS 40.0%
! GAAGGGTATACACCTTTGTT+TGG + chr1.4:18202141-18202160 MS.gene82993:CDS 40.0%
! GAGAGGAGATTTTCCATCAA+AGG + chr1.4:18204376-18204395 MS.gene82993:CDS 40.0%
! GTGTGATTTTGTCAAACTCG+CGG - chr1.4:18203015-18203034 None:intergenic 40.0%
!! TTTGTTCTGTTTTCAGGCTC+TGG + chr1.4:18202306-18202325 MS.gene82993:intron 40.0%
ACCGTCTGATCTTCATCCAA+TGG + chr1.4:18204106-18204125 MS.gene82993:intron 45.0%
CACTTGGTCACATGCTCAAT+AGG - chr1.4:18204536-18204555 None:intergenic 45.0%
CAGATACAAAGTCCCTCTAC+CGG + chr1.4:18204670-18204689 MS.gene82993:CDS 45.0%
CCAAGTAGATGCATCCCATA+TGG - chr1.4:18204420-18204439 None:intergenic 45.0%
CCATATGGGATGCATCTACT+TGG + chr1.4:18204417-18204436 MS.gene82993:CDS 45.0%
CCGGCCATTACCAAATGTAA+CGG - chr1.4:18204744-18204763 None:intergenic 45.0%
CGGCCATTACCAAATGTAAC+GGG - chr1.4:18204743-18204762 None:intergenic 45.0%
GATCAACTGCACAACCATGT+AGG - chr1.4:18204514-18204533 None:intergenic 45.0%
! ACTTTGAGCACTGTCACACT+TGG - chr1.4:18204552-18204571 None:intergenic 45.0%
!! CCGTTACATTTGGTAATGGC+CGG + chr1.4:18204741-18204760 MS.gene82993:CDS 45.0%
!! TAGTGCAATTGATGGCGCAT+TGG - chr1.4:18203111-18203130 None:intergenic 45.0%
!!! GGACTAGTCAGTGTCGTTTT+TGG - chr1.4:18202625-18202644 None:intergenic 45.0%
!!! TCATTTTTTTAAATTATTAT+CGG + chr1.4:18202534-18202553 MS.gene82993:intron 5.0%
ACACAGACACTCCTCGGATT+AGG + chr1.4:18202467-18202486 MS.gene82993:intron 50.0%
CGGTAGAGGGACTTTGTATC+TGG - chr1.4:18204672-18204691 None:intergenic 50.0%
GCATCTACTTGGGCTACCAA+TGG + chr1.4:18204428-18204447 MS.gene82993:CDS 50.0%
TCTACTTGGGCTACCAATGG+AGG + chr1.4:18204431-18204450 MS.gene82993:CDS 50.0%
TGTCAGACACACCTAATCCG+AGG - chr1.4:18202481-18202500 None:intergenic 50.0%
TTGTGGGTTCCTCCCCTTTA+AGG - chr1.4:18203721-18203740 None:intergenic 50.0%
TTTGGCGGTGTCTCTTTCCA+CGG - chr1.4:18204772-18204791 None:intergenic 50.0%
! TCGATTCGCGACAAGCTGAA+AGG + chr1.4:18204702-18204721 MS.gene82993:CDS 50.0%
!! AGTTGGTGGCATGTGACCAT+TGG + chr1.4:18203749-18203768 MS.gene82993:intron 50.0%
CAGCGACACCTCTGATCAAG+AGG - chr1.4:18202703-18202722 None:intergenic 55.0%
CGACACCTCTGATCAAGAGG+TGG - chr1.4:18202700-18202719 None:intergenic 55.0%
CGATCACACACTCCGGTAGA+GGG - chr1.4:18204685-18204704 None:intergenic 55.0%
CGCGAATCGATCACACACTC+CGG - chr1.4:18204692-18204711 None:intergenic 55.0%
GTCAGCCACCTCTTGATCAG+AGG + chr1.4:18202692-18202711 MS.gene82993:intron 55.0%
TCCAACACGACACCAACGAC+CGG - chr1.4:18202671-18202690 None:intergenic 55.0%
TCGATCACACACTCCGGTAG+AGG - chr1.4:18204686-18204705 None:intergenic 55.0%
!! AGGTGTCGCTGCTACAAAGC+TGG + chr1.4:18202712-18202731 MS.gene82993:intron 55.0%
!! CGGTGTTGACGTGTCGTGTT+TGG + chr1.4:18202554-18202573 MS.gene82993:intron 55.0%
!! CGTGTCGTGTTTGGTGTCCA+TGG + chr1.4:18202563-18202582 MS.gene82993:intron 55.0%
GTCTCTTTCCACGGTGGTGC+CGG - chr1.4:18204763-18204782 None:intergenic 60.0%
!! ACCGGTCGTTGGTGTCGTGT+TGG + chr1.4:18202667-18202686 MS.gene82993:intron 60.0%
GGCGGTGTCTCTTTCCACGG+TGG - chr1.4:18204769-18204788 None:intergenic 65.0%
! GGTAATGGCCGGCACCACCG+TGG + chr1.4:18204752-18204771 MS.gene82993:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 18202039 18204811 18202039 ID=MS.gene82993
chr1.4 mRNA 18202039 18204811 18202039 ID=MS.gene82993.t1;Parent=MS.gene82993
chr1.4 exon 18202039 18202228 18202039 ID=MS.gene82993.t1.exon1;Parent=MS.gene82993.t1
chr1.4 CDS 18202039 18202228 18202039 ID=cds.MS.gene82993.t1;Parent=MS.gene82993.t1
chr1.4 exon 18202322 18202422 18202322 ID=MS.gene82993.t1.exon2;Parent=MS.gene82993.t1
chr1.4 CDS 18202322 18202422 18202322 ID=cds.MS.gene82993.t1;Parent=MS.gene82993.t1
chr1.4 exon 18203191 18203399 18203191 ID=MS.gene82993.t1.exon3;Parent=MS.gene82993.t1
chr1.4 CDS 18203191 18203399 18203191 ID=cds.MS.gene82993.t1;Parent=MS.gene82993.t1
chr1.4 exon 18204325 18204811 18204325 ID=MS.gene82993.t1.exon4;Parent=MS.gene82993.t1
chr1.4 CDS 18204325 18204811 18204325 ID=cds.MS.gene82993.t1;Parent=MS.gene82993.t1
Gene Sequence

>MS.gene82993

ATGGAAAAGATTCATATGATGTTGTTCTTGTTCTGTTTTTTTCTTTTTGTTATGCCTTGTTACTCTGTTTCACCAAGAAATTTTCCTATTATACCTTTTGATGAAGGGTATACACCTTTGTTTGGAGACCATAACTTGATCATTCATTCTGATTCCAAAGCTGTTCATCTCTTACTTGATGAGTCTTCAGGTTCTTCATCTTCAATATTAATCTCTTAATTGATTTTAGTGTTAAAATTTAAGTTTTTTTTTTAATATATTTTGTGTTTTGTTCTGTTTTCAGGCTCTGGATTTGCTTCTCATGATCTTTACCTTCATGGCTATTTTAGTGCTTCCATTAAATTACCTTCTGATTATACTGCTGGAGTTGTGGTTGCATTTTATGTGAGTAACACCTCATCATATACTAATGATCTATGAGCTATGAAACACAGACACTCCTCGGATTAGGTGTGTCTGACACGACACTAACACCAAATGTTATAGTGAATTATGTCATTTTTTTAAATTATTATCGGTGTTGACGTGTCGTGTTTGGTGTCCATGGCTCTGTTTCATAGTCTATAAAACACTAATATGGACACCAAAAACGACACTGACTAGTCCACACCTTTAGAAAATGATTTGAACCGGTCGTTGGTGTCGTGTTGGACGTCAGCCACCTCTTGATCAGAGGTGTCGCTGCTACAAAGCTGGTGATGTCATTTTTTTTTTTTTTTAATTTTTGAGACCATTTTTAATATTTTAATTAAATTTTATTGTTTGCAATATTAATTTTTTTTACACTTTCAGTTTCTTTAGTCTTTATGATCAGTAAAGGAAGAACACAAAAAATTAATTATGATTATTATATTTTCTGCTTTTGACCCTTTTTTCCAGCAAAAGTCTGTTTGATATTTTTGAAAGAAACTGTCATTTGGTCTGTTTGTTTTGTCTTGTTCTTCATTTTCAACTTTGTGCATGCTCGAAATCACCGCGAGTTTGACAAAATCACACTGACACCATGATTACGTTGAATTTCTAAATTTTGTTGAATTTAAAAAAAAAGATTGACAAAATCGCGATGGCACCAATGCGCCATCAATTGCACTAATTTTACCTAAAAAATGGAAGAAAGAAATATTAATTTATTCTTAAAATTTTATTCTGCAGTTGTCAAATGGTGACATGTATGAGAAGAACCATGATGAAATAGACTTTGAATTTTTGGGGAACATAAGAGGCAAAGATTGGAGGATTCAAACAAATGTTTATGGAAATGGAAGTACAAACATTGGAAGAGAAGAAAGATATGGTTTATGGTTTGATCCTTCTGAGGAATTTCATCAGTATAGTATTCTTTGGACAGATTCTAAGATAATGTAAGTAAAATCTAATCTACACCCTTAATATGCTATATTTAGTTATTTGATTCTCTTTTTTACTTGTTATAGAAAAAAATTTCTTTATGAGTTGTTTTATTATCATTTATGTTTCACCATTCCCTTGCTTGTACTTTTTGTTCAAAGATGTCTCTGTTTTTCTCCTTTTACCATTTACCACATCCACAGATTTGTGAAAACTGGTTTTTGCTTTTAGTAGGACTATTACTATGTACAATTTTTGCATTGATGGCTTTCTGATAATGTGTAAAAACCATATGTCATCAGAACTTTATATTAAATTTTATGTATATATATCCTTAAAGGGGAGGAACCCACAAGATAAAAAAGTTGGTGGCATGTGACCATTGGATAAATTCCTAGGTATTGGAATCTTTATAAAATTTAGTAGTTGAATAACTATTTTGTCTAACCTAAATTTATGAATTAGCAATCATTATTTGTTCCTTAGTTACCAAGTAGGTTTCATACGTCTTTCCGAACCCTGTTGTAAGAGTTGAGACGATGAACGGATGTTAATAATGAGGTTTAGGGTTTGATCCTAACAAATTAACCATTAACATTTGCCTATAAGAAATATGTATCAGAAGTAACTGCGGCTGCAAATTTCGACTGTTAGATGAAAATCAGTCGAACCGTGTTTTGAGTTGTATTTTAAATCCAATTTAATGATTGAAAAACACGGACCGTCTGATCTTCATCCAATGGTTGACATCTGCAACTATTAGAACTTACGATTGCATATAATCTATTTCATATTTCTTTTATATACATTTGAGAGATAGATGGAAATTATTCTTGATTAACTTTCATTATGGTACTTGTTAGTGTCAAGTTTGATAATTATGTGTATTGTACTTTCTTTATTAATTAAATGATCATATATATTTTTATTCTTTTGCAGATTTTATGTGGATGATGTTCCTATCAGAGAAGTAAAGAGAACTGAGTCAATGAGAGGAGATTTTCCATCAAAGGCAATGACTTTGTATTCTACCATATGGGATGCATCTACTTGGGCTACCAATGGAGGAAAATACAAAGTAAACTACAAATATGCACCCTATATTACTGAATTCTCTGACTTTGTCCTACATGGTTGTGCAGTTGATCCTATTGAGCATGTGACCAAGTGTGACAGTGCTCAAAGTTCAGAAACAATTCCTTCTGATGTTTCACAAGTGGAAAGAATCAAAATGGATAACTTTAGGTTCAAGCACATGACATACTCTTACTGTTATGACAAAACCAGATACAAAGTCCCTCTACCGGAGTGTGTGATCGATTCGCGACAAGCTGAAAGGCTGAGAAAATTTGATCCCGTTACATTTGGTAATGGCCGGCACCACCGTGGAAAGAGACACCGCCAAAACAAAGTTACTTCGTTTTAG
Protein sequence

>MS.gene82993.t1

MEKIHMMLFLFCFFLFVMPCYSVSPRNFPIIPFDEGYTPLFGDHNLIIHSDSKAVHLLLDESSGSGFASHDLYLHGYFSASIKLPSDYTAGVVVAFYLSNGDMYEKNHDEIDFEFLGNIRGKDWRIQTNVYGNGSTNIGREERYGLWFDPSEEFHQYSILWTDSKIIFYVDDVPIREVKRTESMRGDFPSKAMTLYSTIWDASTWATNGGKYKVNYKYAPYITEFSDFVLHGCAVDPIEHVTKCDSAQSSETIPSDVSQVERIKMDNFRFKHMTYSYCYDKTRYKVPLPECVIDSRQAERLRKFDPVTFGNGRHHRGKRHRQNKVTSF