Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83166.t1 | XP_003620613.1 | 88.1 | 84 | 10 | 0 | 80 | 163 | 554 | 637 | 5.90E-31 | 144.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83166.t1 | G7KPE2 | 88.1 | 84 | 10 | 0 | 80 | 163 | 554 | 637 | 4.3e-31 | 144.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049701 | MS.gene83166 | 0.804783 | 1.83E-49 | -1.69E-46 |
| MS.gene049853 | MS.gene83166 | 0.834956 | 2.22E-56 | -1.69E-46 |
| MS.gene052447 | MS.gene83166 | 0.808325 | 3.27E-50 | -1.69E-46 |
| MS.gene054174 | MS.gene83166 | 0.832503 | 9.11E-56 | -1.69E-46 |
| MS.gene054175 | MS.gene83166 | 0.832605 | 8.59E-56 | -1.69E-46 |
| MS.gene054484 | MS.gene83166 | 0.833158 | 6.26E-56 | -1.69E-46 |
| MS.gene055017 | MS.gene83166 | 0.800601 | 1.34E-48 | -1.69E-46 |
| MS.gene055058 | MS.gene83166 | 0.80742 | 5.10E-50 | -1.69E-46 |
| MS.gene058515 | MS.gene83166 | 0.846273 | 2.41E-59 | -1.69E-46 |
| MS.gene059276 | MS.gene83166 | 0.800922 | 1.15E-48 | -1.69E-46 |
| MS.gene059459 | MS.gene83166 | 0.802177 | 6.35E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83166.t1 | MTR_6g087760 | 88.095 | 84 | 10 | 0 | 80 | 163 | 554 | 637 | 1.81e-41 | 147 |
| MS.gene83166.t1 | MTR_6g034670 | 63.542 | 96 | 29 | 2 | 1 | 96 | 1 | 90 | 3.96e-31 | 108 |
| MS.gene83166.t1 | MTR_2g066850 | 73.333 | 60 | 16 | 0 | 5 | 64 | 130 | 189 | 1.32e-20 | 88.2 |
| MS.gene83166.t1 | MTR_1g036590 | 61.905 | 63 | 20 | 1 | 1 | 59 | 34 | 96 | 2.89e-13 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTTTGTGATGGAGAAAAT+AGG | 0.299950 | 8.2:-57670896 | MS.gene83166:CDS |
| ACTTTGTGATGGAGAAAATA+GGG | 0.355897 | 8.2:-57670895 | MS.gene83166:CDS |
| CATCATCAACTAAAGAATCT+TGG | 0.429784 | 8.2:+57670712 | None:intergenic |
| AGGTCACAAGCTTCCTCAGC+AGG | 0.444090 | 8.2:+57670651 | None:intergenic |
| AATTCTTATGCCGTTGAAAG+AGG | 0.471956 | 8.2:-57671033 | MS.gene83166:CDS |
| AAGAGGAGATCGACGCTGCT+TGG | 0.474561 | 8.2:-57671016 | MS.gene83166:CDS |
| CCACAGACGTCGAAAACGCT+TGG | 0.482999 | 8.2:+57670988 | None:intergenic |
| GTCTTCTCAATACTAACAAC+AGG | 0.493869 | 8.2:+57670936 | None:intergenic |
| CTCAATACTAACAACAGGTT+CGG | 0.502695 | 8.2:+57670941 | None:intergenic |
| GGCAGATAACTCGCGTAAGA+CGG | 0.522278 | 8.2:-57671102 | MS.gene83166:CDS |
| CACAGACGTCGAAAACGCTT+GGG | 0.530092 | 8.2:+57670989 | None:intergenic |
| ACTAGACATAATTGCGAAGA+GGG | 0.530232 | 8.2:-57670735 | MS.gene83166:CDS |
| ATGTCTAGTCTTATTTGCAT+AGG | 0.531421 | 8.2:+57670749 | None:intergenic |
| CACATGGTTTCACTTTGTGA+TGG | 0.540604 | 8.2:-57670906 | MS.gene83166:CDS |
| ACAAGCTTCCTCAGCAGGGA+TGG | 0.565314 | 8.2:+57670656 | None:intergenic |
| TGAGAAGACTGGTGAGCACA+TGG | 0.589881 | 8.2:-57670922 | MS.gene83166:CDS |
| GTTGTTAGTATTGAGAAGAC+TGG | 0.592091 | 8.2:-57670933 | MS.gene83166:CDS |
| GAGGAAGCTTGTGACCTCAC+CGG | 0.594168 | 8.2:-57670645 | MS.gene83166:CDS |
| GAAACAAGTTTAACTGACAC+CGG | 0.639197 | 8.2:+57670626 | None:intergenic |
| GACTAGACATAATTGCGAAG+AGG | 0.643960 | 8.2:-57670736 | MS.gene83166:CDS |
| AATTGATACCATCCCTGCTG+AGG | 0.644195 | 8.2:-57670664 | MS.gene83166:CDS |
| GCGTCGATCTCCTCTTTCAA+CGG | 0.670207 | 8.2:+57671023 | None:intergenic |
| GGTCACAAGCTTCCTCAGCA+GGG | 0.670761 | 8.2:+57670652 | None:intergenic |
| AACAGAAGATCTCATCGTGG+AGG | 0.674719 | 8.2:-57670784 | MS.gene83166:CDS |
| AAGTTTAACTGACACCGGTG+AGG | 0.700035 | 8.2:+57670631 | None:intergenic |
| AGTAACAGAAGATCTCATCG+TGG | 0.778770 | 8.2:-57670787 | MS.gene83166:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATTAAAGCAAGAAGAA+GGG | - | chr8.2:57670873-57670892 | MS.gene83166:CDS | 20.0% |
| ! | GAAAAATTAAAGCAAGAAGA+AGG | - | chr8.2:57670872-57670891 | MS.gene83166:CDS | 25.0% |
| CATCATCAACTAAAGAATCT+TGG | + | chr8.2:57671030-57671049 | None:intergenic | 30.0% | |
| ! | ACTTTGTGATGGAGAAAATA+GGG | - | chr8.2:57670844-57670863 | MS.gene83166:CDS | 30.0% |
| !! | ATGTCTAGTCTTATTTGCAT+AGG | + | chr8.2:57670993-57671012 | None:intergenic | 30.0% |
| AATTCTTATGCCGTTGAAAG+AGG | - | chr8.2:57670706-57670725 | MS.gene83166:CDS | 35.0% | |
| ACTAGACATAATTGCGAAGA+GGG | - | chr8.2:57671004-57671023 | MS.gene83166:CDS | 35.0% | |
| CTCAATACTAACAACAGGTT+CGG | + | chr8.2:57670801-57670820 | None:intergenic | 35.0% | |
| GTCTTCTCAATACTAACAAC+AGG | + | chr8.2:57670806-57670825 | None:intergenic | 35.0% | |
| ! | CACTTTGTGATGGAGAAAAT+AGG | - | chr8.2:57670843-57670862 | MS.gene83166:CDS | 35.0% |
| ! | GTTGTTAGTATTGAGAAGAC+TGG | - | chr8.2:57670806-57670825 | MS.gene83166:CDS | 35.0% |
| AGTAACAGAAGATCTCATCG+TGG | - | chr8.2:57670952-57670971 | MS.gene83166:CDS | 40.0% | |
| CACATGGTTTCACTTTGTGA+TGG | - | chr8.2:57670833-57670852 | MS.gene83166:CDS | 40.0% | |
| GACTAGACATAATTGCGAAG+AGG | - | chr8.2:57671003-57671022 | MS.gene83166:CDS | 40.0% | |
| AACAGAAGATCTCATCGTGG+AGG | - | chr8.2:57670955-57670974 | MS.gene83166:CDS | 45.0% | |
| AATTGATACCATCCCTGCTG+AGG | - | chr8.2:57671075-57671094 | MS.gene83166:CDS | 45.0% | |
| ! | GTTTTCGACGTCTGTGGAAT+CGG | - | chr8.2:57670757-57670776 | MS.gene83166:CDS | 45.0% |
| CACAGACGTCGAAAACGCTT+GGG | + | chr8.2:57670753-57670772 | None:intergenic | 50.0% | |
| GGCAGATAACTCGCGTAAGA+CGG | - | chr8.2:57670637-57670656 | MS.gene83166:CDS | 50.0% | |
| TGAGAAGACTGGTGAGCACA+TGG | - | chr8.2:57670817-57670836 | MS.gene83166:CDS | 50.0% | |
| ! | GCGTCGATCTCCTCTTTCAA+CGG | + | chr8.2:57670719-57670738 | None:intergenic | 50.0% |
| AAGAGGAGATCGACGCTGCT+TGG | - | chr8.2:57670723-57670742 | MS.gene83166:CDS | 55.0% | |
| ACAAGCTTCCTCAGCAGGGA+TGG | + | chr8.2:57671086-57671105 | None:intergenic | 55.0% | |
| AGGTCACAAGCTTCCTCAGC+AGG | + | chr8.2:57671091-57671110 | None:intergenic | 55.0% | |
| CCACAGACGTCGAAAACGCT+TGG | + | chr8.2:57670754-57670773 | None:intergenic | 55.0% | |
| GAGGAAGCTTGTGACCTCAC+CGG | - | chr8.2:57671094-57671113 | MS.gene83166:CDS | 55.0% | |
| GGTCACAAGCTTCCTCAGCA+GGG | + | chr8.2:57671090-57671109 | None:intergenic | 55.0% | |
| ! | CCAAGCGTTTTCGACGTCTG+TGG | - | chr8.2:57670751-57670770 | MS.gene83166:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 57670635 | 57671126 | 57670635 | ID=MS.gene83166 |
| chr8.2 | mRNA | 57670635 | 57671126 | 57670635 | ID=MS.gene83166.t1;Parent=MS.gene83166 |
| chr8.2 | exon | 57670635 | 57671126 | 57670635 | ID=MS.gene83166.t1.exon1;Parent=MS.gene83166.t1 |
| chr8.2 | CDS | 57670635 | 57671126 | 57670635 | ID=cds.MS.gene83166.t1;Parent=MS.gene83166.t1 |
| Gene Sequence |
| Protein sequence |
