AlfalfaGEDB | MS.gene83291

M. sativa cultivar XinJiangDaYe / MS.gene83291

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00197.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83291.t1	XP_013452627.1	80.6	217	39	1	4	217	1	217	8.50E-94	353.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83291.t1	Q41015	54.6	207	87	3	4	205	1	205	6.7e-55	215.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83291.t1	B7FN38	80.6	217	39	1	4	217	1	217	6.2e-94	353.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050338	MS.gene83291	0.817979	2.49E-52	-1.69E-46
MS.gene051439	MS.gene83291	0.829728	4.38E-55	-1.69E-46
MS.gene051517	MS.gene83291	0.800032	1.74E-48	-1.69E-46
MS.gene052129	MS.gene83291	0.800609	1.33E-48	-1.69E-46
MS.gene05470	MS.gene83291	-0.80489	1.74E-49	-1.69E-46
MS.gene055616	MS.gene83291	0.800796	1.22E-48	-1.69E-46
MS.gene05589	MS.gene83291	-0.808815	2.57E-50	-1.69E-46
MS.gene056694	MS.gene83291	0.825193	5.36E-54	-1.69E-46
MS.gene056697	MS.gene83291	0.82648	2.65E-54	-1.69E-46
MS.gene057717	MS.gene83291	0.816058	6.73E-52	-1.69E-46
MS.gene05926	MS.gene83291	0.823669	1.22E-53	-1.69E-46
MS.gene059304	MS.gene83291	0.804854	1.77E-49	-1.69E-46
MS.gene060087	MS.gene83291	0.809056	2.28E-50	-1.69E-46
MS.gene061044	MS.gene83291	0.847059	1.47E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83291.t1	MTR_6g066190	80.645	217	39	1	4	217	1	217	1.31e-125	354
MS.gene83291.t1	MTR_6g066210	79.167	216	43	1	4	217	1	216	3.58e-125	353
MS.gene83291.t1	MTR_6g009650	78.505	214	42	1	4	217	1	210	5.12e-122	344
MS.gene83291.t1	MTR_6g009563	77.833	203	44	1	4	205	1	203	1.50e-115	328
MS.gene83291.t1	MTR_6g059730	56.872	211	85	3	4	210	1	209	4.80e-68	208
MS.gene83291.t1	MTR_6g059680	57.009	214	86	3	4	211	1	214	1.40e-60	189
MS.gene83291.t1	MTR_6g017225	51.835	218	91	8	4	211	1	214	2.87e-59	191
MS.gene83291.t1	MTR_1g035770	44.444	216	108	7	4	209	1	214	4.51e-49	160
MS.gene83291.t1	MTR_6g059810	44.907	216	107	6	1	207	1	213	8.49e-47	154
MS.gene83291.t1	MTR_6g065460	46.667	210	98	7	4	205	1	204	2.71e-39	134
MS.gene83291.t1	MTR_6g059650	41.848	184	94	6	30	206	26	203	2.55e-32	116
MS.gene83291.t1	MTR_6g059530	43.750	208	98	9	4	205	1	195	2.90e-32	116
MS.gene83291.t1	MTR_6g059410	42.927	205	99	8	8	205	1	194	1.19e-30	112
MS.gene83291.t1	MTR_6g065460	41.546	207	89	6	4	205	1	180	6.11e-29	107
MS.gene83291.t1	MTR_6g059760	40.271	221	114	10	4	215	1	212	8.02e-29	107
MS.gene83291.t1	MTR_3g463590	38.421	190	100	7	32	212	29	210	1.89e-22	91.3
MS.gene83291.t1	MTR_6g044780	36.444	225	118	12	4	212	1	216	4.53e-22	90.5
MS.gene83291.t1	MTR_6g044810	37.391	230	109	13	4	212	1	216	8.29e-22	89.7
MS.gene83291.t1	MTR_6g045097	36.889	225	117	12	4	212	1	216	8.66e-22	89.7
MS.gene83291.t1	MTR_6g045433	36.752	234	110	11	4	212	1	221	1.09e-21	89.4
MS.gene83291.t1	MTR_6g045403	36.239	218	115	9	4	208	1	207	1.25e-21	89.4
MS.gene83291.t1	MTR_1g057110	35.238	210	108	13	4	207	1	188	2.66e-16	74.3
MS.gene83291.t1	MTR_2g072980	33.175	211	109	12	4	203	5	194	6.38e-13	65.5
MS.gene83291.t1	MTR_6g078070	31.863	204	125	9	10	205	6	203	1.24e-12	64.7
MS.gene83291.t1	MTR_6g065570	38.750	80	40	1	22	92	17	96	3.60e-12	60.8
MS.gene83291.t1	MTR_2g073010	32.394	213	116	11	3	205	4	198	9.47e-12	62.0
MS.gene83291.t1	MTR_6g059755	38.462	130	70	6	91	215	5	129	7.40e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83291.t1	AT1G17860	32.020	203	122	8	9	205	3	195	1.21e-13	67.4

Find 55 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATACTAGGGTTATCACTTTC+TGG	0.161357	6.1:+62581171	None:intergenic
CTGAATCACCAACTTTGTTT+AGG	0.216525	6.1:+62581136	None:intergenic
ATGGATCTGGTAATGTTTAT+AGG	0.227385	6.1:-62580855	MS.gene83291:CDS
ATACAAGATATTGCTGTAAT+AGG	0.268557	6.1:-62581096	MS.gene83291:CDS
CCTCTGATTGTCTCTATTCC+AGG	0.274561	6.1:+62580898	None:intergenic
ACTGGAGATTGTGCTTATAT+TGG	0.278706	6.1:-62580811	MS.gene83291:CDS
TCTAGTATGGTGAATTTAAC+TGG	0.302951	6.1:+62581069	None:intergenic
TGAATCACCAACTTTGTTTA+GGG	0.308935	6.1:+62581137	None:intergenic
AGCATTGTTTGGTGAAATAT+TGG	0.343828	6.1:+62581257	None:intergenic
GAGGGATAAAATAGGTTTCA+TGG	0.353544	6.1:+62581311	None:intergenic
GAGCAAGTATTGGACATAAA+TGG	0.360089	6.1:-62581231	MS.gene83291:CDS
GGACTGCAACTGTTTAGTTC+TGG	0.363850	6.1:-62580790	MS.gene83291:CDS
TACTAGAACTCATACTAAAT+AGG	0.365223	6.1:+62580673	None:intergenic
TGGATCTGGTAATGTTTATA+GGG	0.365536	6.1:-62580854	MS.gene83291:CDS
GATGCTGCATCTATTAAATC+TGG	0.371349	6.1:-62580709	MS.gene83291:CDS
TCTATTCCAGGATAGTTTGC+AGG	0.388966	6.1:+62580910	None:intergenic
TATTGGTGATAAAAGCAAAT+AGG	0.390605	6.1:+62581274	None:intergenic
TTTGATGATTCAGCACAATC+TGG	0.398835	6.1:+62580988	None:intergenic
ACTTGCTCAACAGCATTGTT+TGG	0.401563	6.1:+62581246	None:intergenic
TCTGGTAATGTTTATAGGGT+TGG	0.416063	6.1:-62580850	MS.gene83291:CDS
GAAAGGGTGAGGGATAAAAT+AGG	0.419184	6.1:+62581303	None:intergenic
CAACTAGCTTGTGTTGGTAT+TGG	0.427835	6.1:-62580937	MS.gene83291:CDS
CTAGCTTGTGTTGGTATTGG+TGG	0.441922	6.1:-62580934	MS.gene83291:CDS
TATTGGCCACCAGGGGTAAG+GGG	0.451454	6.1:+62581204	None:intergenic
GGTGGTCCTGCAAACTATCC+TGG	0.456364	6.1:-62580916	MS.gene83291:CDS
TTAATAGTGAAGCATGGATC+TGG	0.460803	6.1:-62580868	MS.gene83291:CDS
AGTATTGGCCACCAGGGGTA+AGG	0.463701	6.1:+62581202	None:intergenic
AGGGTTAGTCCTCCAATACT+AGG	0.474991	6.1:+62581156	None:intergenic
ATTGGCCACCAGGGGTAAGG+GGG	0.476496	6.1:+62581205	None:intergenic
AGCAAATAGGAAGAAGGAAA+GGG	0.481208	6.1:+62581287	None:intergenic
CAATGCTGTTGAGCAAGTAT+TGG	0.481709	6.1:-62581241	MS.gene83291:CDS
TTTCTGGTAAAATGTAGTAT+TGG	0.484403	6.1:+62581187	None:intergenic
AAGCAAATAGGAAGAAGGAA+AGG	0.491844	6.1:+62581286	None:intergenic
GAAAGTGATAACCCTAGTAT+TGG	0.495230	6.1:-62581168	MS.gene83291:CDS
AAAATGTAGTATTGGCCACC+AGG	0.496116	6.1:+62581195	None:intergenic
ATTGTGCTGAATCATCAAAA+TGG	0.497723	6.1:-62580984	MS.gene83291:CDS
GTATTGGCCACCAGGGGTAA+GGG	0.502071	6.1:+62581203	None:intergenic
AGTGATAACCCTAGTATTGG+AGG	0.519798	6.1:-62581165	MS.gene83291:CDS
GATAAAAGCAAATAGGAAGA+AGG	0.526672	6.1:+62581281	None:intergenic
ATATCTTGTATGACAGTGAC+TGG	0.527567	6.1:+62581108	None:intergenic
CTGTTTAGTTCTGGTGAAGG+AGG	0.540270	6.1:-62580781	MS.gene83291:CDS
ATAGGAAGAAGGAAAGGGTG+AGG	0.540395	6.1:+62581292	None:intergenic
GGACTAACCCTAAACAAAGT+TGG	0.540688	6.1:-62581144	MS.gene83291:CDS
GGGTTAGTCCTCCAATACTA+GGG	0.545234	6.1:+62581157	None:intergenic
ATCACTAGTAACATCGTGAC+AGG	0.548264	6.1:-62581042	MS.gene83291:CDS
ATACAACAGAATAAGCATCA+AGG	0.559870	6.1:+62580737	None:intergenic
TAGGAAGAAGGAAAGGGTGA+GGG	0.562566	6.1:+62581293	None:intergenic
TACAACAGAATAAGCATCAA+GGG	0.562914	6.1:+62580738	None:intergenic
TTGGCCACCAGGGGTAAGGG+GGG	0.568615	6.1:+62581206	None:intergenic
AGTGAACTCGATCTCAAGAT+CGG	0.599342	6.1:+62581017	None:intergenic
AATACTCAACTAGCTTGTGT+TGG	0.603192	6.1:-62580943	MS.gene83291:CDS
CAACTGTTTAGTTCTGGTGA+AGG	0.626732	6.1:-62580784	MS.gene83291:CDS
AAATGTAGTATTGGCCACCA+GGG	0.645099	6.1:+62581196	None:intergenic
CCTGGAATAGAGACAATCAG+AGG	0.673236	6.1:-62580898	MS.gene83291:CDS
AATGTAGTATTGGCCACCAG+GGG	0.700291	6.1:+62581197	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATACAAGATATTGCTGTAAT+AGG	-	chr6.1:62580909-62580928	MS.gene83291:CDS	25.0%
!!	AAGTTTTTAATAGTGAAGCA+TGG	-	chr6.1:62581131-62581150	MS.gene83291:CDS	25.0%
!!	TATTGGTGATAAAAGCAAAT+AGG	+	chr6.1:62580734-62580753	None:intergenic	25.0%
!!	TTTCTGGTAAAATGTAGTAT+TGG	+	chr6.1:62580821-62580840	None:intergenic	25.0%
	ATACAACAGAATAAGCATCA+AGG	+	chr6.1:62581271-62581290	None:intergenic	30.0%
	ATTGTGCTGAATCATCAAAA+TGG	-	chr6.1:62581021-62581040	MS.gene83291:CDS	30.0%
	GATAAAAGCAAATAGGAAGA+AGG	+	chr6.1:62580727-62580746	None:intergenic	30.0%
	TACAACAGAATAAGCATCAA+GGG	+	chr6.1:62581270-62581289	None:intergenic	30.0%
	TCTAGTATGGTGAATTTAAC+TGG	+	chr6.1:62580939-62580958	None:intergenic	30.0%
!	AGCATTGTTTGGTGAAATAT+TGG	+	chr6.1:62580751-62580770	None:intergenic	30.0%
!	ATGGATCTGGTAATGTTTAT+AGG	-	chr6.1:62581150-62581169	MS.gene83291:CDS	30.0%
!	TGAATCACCAACTTTGTTTA+GGG	+	chr6.1:62580871-62580890	None:intergenic	30.0%
!	TGGATCTGGTAATGTTTATA+GGG	-	chr6.1:62581151-62581170	MS.gene83291:CDS	30.0%
!	TTATAGGGTTGGATTTTGTT+TGG	-	chr6.1:62581166-62581185	MS.gene83291:CDS	30.0%
!!	ACTAGTGATGTTTTCTAGTA+TGG	+	chr6.1:62580952-62580971	None:intergenic	30.0%
	AAGCAAATAGGAAGAAGGAA+AGG	+	chr6.1:62580722-62580741	None:intergenic	35.0%
	ACTGGAGATTGTGCTTATAT+TGG	-	chr6.1:62581194-62581213	MS.gene83291:CDS	35.0%
	AGCAAATAGGAAGAAGGAAA+GGG	+	chr6.1:62580721-62580740	None:intergenic	35.0%
	ATACTAGGGTTATCACTTTC+TGG	+	chr6.1:62580837-62580856	None:intergenic	35.0%
	ATATCTTGTATGACAGTGAC+TGG	+	chr6.1:62580900-62580919	None:intergenic	35.0%
	GAAAGTGATAACCCTAGTAT+TGG	-	chr6.1:62580837-62580856	MS.gene83291:CDS	35.0%
	GAGGGATAAAATAGGTTTCA+TGG	+	chr6.1:62580697-62580716	None:intergenic	35.0%
	GATGCTGCATCTATTAAATC+TGG	-	chr6.1:62581296-62581315	MS.gene83291:CDS	35.0%
	TTAATAGTGAAGCATGGATC+TGG	-	chr6.1:62581137-62581156	MS.gene83291:CDS	35.0%
!	AATACTCAACTAGCTTGTGT+TGG	-	chr6.1:62581062-62581081	MS.gene83291:CDS	35.0%
!	CTGAATCACCAACTTTGTTT+AGG	+	chr6.1:62580872-62580891	None:intergenic	35.0%
!	TCTGGTAATGTTTATAGGGT+TGG	-	chr6.1:62581155-62581174	MS.gene83291:CDS	35.0%
!	TTTGATGATTCAGCACAATC+TGG	+	chr6.1:62581020-62581039	None:intergenic	35.0%
!!	GAGCAAGTATTGGACATAAA+TGG	-	chr6.1:62580774-62580793	MS.gene83291:CDS	35.0%
!!	GGATTTTGTTTGGATACAAC+TGG	-	chr6.1:62581176-62581195	MS.gene83291:CDS	35.0%
	ACTTGCTCAACAGCATTGTT+TGG	+	chr6.1:62580762-62580781	None:intergenic	40.0%
	AGTGAACTCGATCTCAAGAT+CGG	+	chr6.1:62580991-62581010	None:intergenic	40.0%
	ATCACTAGTAACATCGTGAC+AGG	-	chr6.1:62580963-62580982	MS.gene83291:CDS	40.0%
	CAATGCTGTTGAGCAAGTAT+TGG	-	chr6.1:62580764-62580783	MS.gene83291:CDS	40.0%
	GAAAGGGTGAGGGATAAAAT+AGG	+	chr6.1:62580705-62580724	None:intergenic	40.0%
	GGACTAACCCTAAACAAAGT+TGG	-	chr6.1:62580861-62580880	MS.gene83291:CDS	40.0%
!	AGTGATAACCCTAGTATTGG+AGG	-	chr6.1:62580840-62580859	MS.gene83291:CDS	40.0%
!	CAACTAGCTTGTGTTGGTAT+TGG	-	chr6.1:62581068-62581087	MS.gene83291:CDS	40.0%
!	TCTATTCCAGGATAGTTTGC+AGG	+	chr6.1:62581098-62581117	None:intergenic	40.0%
!!	AAAATGTAGTATTGGCCACC+AGG	+	chr6.1:62580813-62580832	None:intergenic	40.0%
!!	AAATGTAGTATTGGCCACCA+GGG	+	chr6.1:62580812-62580831	None:intergenic	40.0%
!!	CAACTGTTTAGTTCTGGTGA+AGG	-	chr6.1:62581221-62581240	MS.gene83291:CDS	40.0%
	ATAGGAAGAAGGAAAGGGTG+AGG	+	chr6.1:62580716-62580735	None:intergenic	45.0%
	CCTCTGATTGTCTCTATTCC+AGG	+	chr6.1:62581110-62581129	None:intergenic	45.0%
	CCTGGAATAGAGACAATCAG+AGG	-	chr6.1:62581107-62581126	MS.gene83291:CDS	45.0%
	TAGGAAGAAGGAAAGGGTGA+GGG	+	chr6.1:62580715-62580734	None:intergenic	45.0%
!	CTAGCTTGTGTTGGTATTGG+TGG	-	chr6.1:62581071-62581090	MS.gene83291:CDS	45.0%
!	GGACTGCAACTGTTTAGTTC+TGG	-	chr6.1:62581215-62581234	MS.gene83291:CDS	45.0%
!!	AATGTAGTATTGGCCACCAG+GGG	+	chr6.1:62580811-62580830	None:intergenic	45.0%
!!	AGGGTTAGTCCTCCAATACT+AGG	+	chr6.1:62580852-62580871	None:intergenic	45.0%
!!	CTGTTTAGTTCTGGTGAAGG+AGG	-	chr6.1:62581224-62581243	MS.gene83291:CDS	45.0%
!!	GGGTTAGTCCTCCAATACTA+GGG	+	chr6.1:62580851-62580870	None:intergenic	45.0%
	GGTGGTCCTGCAAACTATCC+TGG	-	chr6.1:62581089-62581108	MS.gene83291:CDS	55.0%
	TATTGGCCACCAGGGGTAAG+GGG	+	chr6.1:62580804-62580823	None:intergenic	55.0%
!	GTATTGGCCACCAGGGGTAA+GGG	+	chr6.1:62580805-62580824	None:intergenic	55.0%
!!	AGTATTGGCCACCAGGGGTA+AGG	+	chr6.1:62580806-62580825	None:intergenic	55.0%
	AATGGTACCCCCCTTACCCC+TGG	-	chr6.1:62580792-62580811	MS.gene83291:CDS	60.0%
	ATTGGCCACCAGGGGTAAGG+GGG	+	chr6.1:62580803-62580822	None:intergenic	60.0%
	TTGGCCACCAGGGGTAAGGG+GGG	+	chr6.1:62580802-62580821	None:intergenic	65.0%
	GGTACCCCCCTTACCCCTGG+TGG	-	chr6.1:62580795-62580814	MS.gene83291:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	62580687	62581340	62580687	ID=MS.gene83291
chr6.1	mRNA	62580687	62581340	62580687	ID=MS.gene83291.t1;Parent=MS.gene83291
chr6.1	exon	62580687	62581340	62580687	ID=MS.gene83291.t1.exon1;Parent=MS.gene83291.t1
chr6.1	CDS	62580687	62581340	62580687	ID=cds.MS.gene83291.t1;Parent=MS.gene83291.t1

Gene Sequence

>MS.gene83291

ATGGAAACCATGAAACCTATTTTATCCCTCACCCTTTCCTTCTTCCTATTTGCTTTTATCACCAATATTTCACCAAACAATGCTGTTGAGCAAGTATTGGACATAAATGGTACCCCCCTTACCCCTGGTGGCCAATACTACATTTTACCAGAAAGTGATAACCCTAGTATTGGAGGACTAACCCTAAACAAAGTTGGTGATTCAGAGTGTCCAGTCACTGTCATACAAGATATTGCTGTAATAGGTTTACCAGTTAAATTCACCATACTAGAAAACATCACTAGTAACATCGTGACAGGTACCGATCTTGAGATCGAGTTCACTAAGAAGCCAGATTGTGCTGAATCATCAAAATGGTTGATGTTTGTTGATCATAATACTCAACTAGCTTGTGTTGGTATTGGTGGTCCTGCAAACTATCCTGGAATAGAGACAATCAGAGGAAAGTTTTTAATAGTGAAGCATGGATCTGGTAATGTTTATAGGGTTGGATTTTGTTTGGATACAACTGGAGATTGTGCTTATATTGGACTGCAACTGTTTAGTTCTGGTGAAGGAGGATCACGTTTAATTTTGACTGCCCTTGATGCTTATTCTGTTGTATTTGTTGATGCTGCATCTATTAAATCTGGATCACGTGCATTGCCTATTTAG

Protein sequence

>MS.gene83291.t1

METMKPILSLTLSFFLFAFITNISPNNAVEQVLDINGTPLTPGGQYYILPESDNPSIGGLTLNKVGDSECPVTVIQDIAVIGLPVKFTILENITSNIVTGTDLEIEFTKKPDCAESSKWLMFVDHNTQLACVGIGGPANYPGIETIRGKFLIVKHGSGNVYRVGFCLDTTGDCAYIGLQLFSSGEGGSRLILTALDAYSVVFVDAASIKSGSRALPI