AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83521

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83521.t1 MTR_2g105390 83.465 381 14 7 1 352 1 361 0.0 600
MS.gene83521.t1 MTR_1g022370 28.102 274 130 12 131 350 161 421 2.28e-14 74.3
MS.gene83521.t1 MTR_2g020280 53.226 62 22 2 296 350 232 293 8.50e-13 68.6
MS.gene83521.t1 MTR_2g020280 53.226 62 22 2 296 350 331 392 1.21e-12 68.9
MS.gene83521.t1 MTR_1g088960 50.000 62 24 1 296 350 340 401 1.84e-12 68.2
MS.gene83521.t1 MTR_2g007930 40.909 110 44 5 256 350 323 426 4.09e-12 67.4
MS.gene83521.t1 MTR_5g020580 38.947 95 44 3 265 350 326 415 7.49e-12 66.6
MS.gene83521.t1 MTR_8g077195 36.697 109 54 4 251 350 309 411 1.82e-11 65.5
MS.gene83521.t1 MTR_1g098790 36.207 116 47 4 256 350 321 430 2.50e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83521.t1 AT1G16070 54.407 329 119 7 50 352 74 397 3.63e-118 347
MS.gene83521.t1 AT1G16070 51.957 281 104 7 50 304 74 349 2.91e-88 270
MS.gene83521.t1 AT1G16070 50.450 222 79 7 50 245 74 290 2.47e-63 204
MS.gene83521.t1 AT2G18280 27.132 258 134 8 138 350 144 392 2.32e-15 77.0
MS.gene83521.t1 AT2G18280 27.132 258 134 8 138 350 144 392 2.32e-15 77.0
MS.gene83521.t1 AT1G47270 26.792 265 137 11 131 350 160 412 1.46e-12 68.6
MS.gene83521.t1 AT1G47270 26.792 265 137 11 131 350 135 387 1.51e-12 68.6
MS.gene83521.t1 AT1G47270 26.792 265 137 11 131 350 159 411 1.59e-12 68.6
MS.gene83521.t1 AT2G47900 53.226 62 22 2 296 350 345 406 1.78e-12 68.6
MS.gene83521.t1 AT2G47900 53.226 62 22 2 296 350 344 405 1.83e-12 68.6
MS.gene83521.t1 AT2G47900 53.226 62 22 2 296 350 344 405 1.83e-12 68.6
MS.gene83521.t1 AT1G25280 44.156 77 40 2 275 350 192 266 3.19e-12 66.6
MS.gene83521.t1 AT1G25280 44.156 77 40 2 275 350 192 266 3.19e-12 66.6
MS.gene83521.t1 AT1G25280 31.884 138 69 3 233 350 312 444 1.16e-11 66.2
MS.gene83521.t1 AT3G06380 45.902 61 27 1 296 350 227 287 5.00e-11 63.2

Find 99 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCAGCTCTTTGAAAATT+TGG 0.061965 2.2:+325063 MS.gene83521:CDS
GTTGAAAATGAGTTGGATTT+TGG 0.248838 2.2:-324957 None:intergenic
GTTTACTACTGCGGGGCTTA+TGG 0.274791 2.2:-325558 None:intergenic
TTCCAGCTCTTTGAAAATTT+GGG 0.299500 2.2:+325064 MS.gene83521:CDS
CCTGTACATGTTGCTATTGT+AGG 0.305231 2.2:-325683 None:intergenic
AAACTTTCAACTCACGTTAG+AGG 0.308085 2.2:+326169 MS.gene83521:CDS
TACGACCAAGGGTTTGGTTT+TGG 0.321955 2.2:-324833 None:intergenic
CCGGTTCGAAATACCACATT+TGG 0.324387 2.2:+325518 MS.gene83521:CDS
GAGCTTGACTATAGAGATAA+AGG 0.329596 2.2:+326113 MS.gene83521:CDS
GGTTCAGGTACAACACATTA+AGG 0.334401 2.2:+325901 MS.gene83521:intron
AGACCATTGCCTATAGATAT+TGG 0.366407 2.2:+325158 MS.gene83521:CDS
TTAAGGGACTGCCAATGAAT+TGG 0.380340 2.2:+325918 MS.gene83521:CDS
GCAACAAAGCTTTGCATCAA+TGG 0.384089 2.2:-326495 None:intergenic
TCCCACTCCGGCGCTTGTGG+TGG 0.398163 2.2:-325399 None:intergenic
GTGTACTTGTGTTATTGTTA+AGG 0.401096 2.2:+325184 MS.gene83521:CDS
ACCTCCCACTCCGGCGCTTG+TGG 0.404570 2.2:-325402 None:intergenic
GAAATACCACATTTGGGATC+AGG 0.408006 2.2:+325525 MS.gene83521:CDS
AAAGACATAGATTGATGATT+GGG 0.409134 2.2:-325734 None:intergenic
AAAACATACGACCAAGGGTT+TGG 0.409386 2.2:-324839 None:intergenic
ATATCCTTTGACTGGTTACC+AGG 0.411071 2.2:+326451 MS.gene83521:CDS
TTCCTACCCTCCCTAGCTGC+AGG 0.422680 2.2:-326309 None:intergenic
CTGCTGCTCCTGCTTCCTCC+TGG 0.428096 2.2:+325105 MS.gene83521:CDS
TGTTAAGGAACCAACTCCTC+AGG 0.436248 2.2:+325199 MS.gene83521:CDS
GAAAGACATAGATTGATGAT+TGG 0.438091 2.2:-325735 None:intergenic
TCTACATATCAAAGACTTGT+TGG 0.438291 2.2:-325138 None:intergenic
GTACACCTTCCAATATCTAT+AGG 0.439094 2.2:-325167 None:intergenic
TAAGGGACTGCCAATGAATT+GGG 0.444518 2.2:+325919 MS.gene83521:CDS
CGGTTCGAAATACCACATTT+GGG 0.453880 2.2:+325519 MS.gene83521:CDS
GTTTGCTATGATTAGGAGAA+TGG 0.459646 2.2:+326274 MS.gene83521:intron
GATAAAGGAAGGCCAGGACT+TGG 0.466820 2.2:+326128 MS.gene83521:CDS
AAGAGGTGGTTTACTACTGC+GGG 0.474800 2.2:-325566 None:intergenic
GGGACGGCAGAATAGGAAAC+TGG 0.475585 2.2:+325369 MS.gene83521:CDS
AAGGTCAGGGACGGCAGAAT+AGG 0.479149 2.2:+325362 MS.gene83521:intron
CCCACTCCGGCGCTTGTGGT+GGG 0.479209 2.2:-325398 None:intergenic
GAAGAATCAGAAGAAGGAAT+AGG 0.486222 2.2:-325035 None:intergenic
CTACATATCAAAGACTTGTT+GGG 0.489447 2.2:-325137 None:intergenic
CGTGTTTGTTGAAAATGAGT+TGG 0.490098 2.2:-324964 None:intergenic
ATCTGCAGATATCCTTTGAC+TGG 0.494012 2.2:+326443 MS.gene83521:intron
CCAAATGTGGTATTTCGAAC+CGG 0.504282 2.2:-325518 None:intergenic
CCGGAGAGACCCTGAGGAGT+TGG 0.514963 2.2:-325209 None:intergenic
TTTCGAACCGGTGAGGTTAG+CGG 0.516957 2.2:-325506 None:intergenic
AAATACCACATTTGGGATCA+GGG 0.518347 2.2:+325526 MS.gene83521:CDS
AAAGTAGACCAGGAGGAAGC+AGG 0.520011 2.2:-325113 None:intergenic
AGCAGACAATCCTGCAGCTA+GGG 0.521878 2.2:+326299 MS.gene83521:CDS
GGAAGCCAAACATATGCAAA+AGG 0.524760 2.2:-326474 None:intergenic
TTTCAAAGAGCTGGAAAAGT+GGG 0.527227 2.2:-325057 None:intergenic
CCTCCTACCTTGTTATAGAG+TGG 0.527819 2.2:-325983 None:intergenic
TATAGAGATAAAGGAAGGCC+AGG 0.527875 2.2:+326122 MS.gene83521:CDS
AAGCAGACAATCCTGCAGCT+AGG 0.533935 2.2:+326298 MS.gene83521:CDS
CTTGTTGGGTAAAGTAGACC+AGG 0.539180 2.2:-325123 None:intergenic
TTTGCTATGATTAGGAGAAT+GGG 0.539684 2.2:+326275 MS.gene83521:intron
AAGTGGGAAGAATCAGAAGA+AGG 0.539717 2.2:-325041 None:intergenic
CTGTGGCCCACCACAAGCGC+CGG 0.547264 2.2:+325392 MS.gene83521:CDS
AAGTGCATCAACTATTCTCA+AGG 0.551216 2.2:+325957 MS.gene83521:CDS
GTTCAGGTACAACACATTAA+GGG 0.551587 2.2:+325902 MS.gene83521:intron
GATCTCTGGATTCCAAGTCC+TGG 0.553307 2.2:-326140 None:intergenic
AGACAATCCTGCAGCTAGGG+AGG 0.560928 2.2:+326302 MS.gene83521:CDS
GTCTTTCAAGAACACCAACC+AGG 0.561985 2.2:+325751 MS.gene83521:CDS
CCTACAATAGCAACATGTAC+AGG 0.562677 2.2:+325683 MS.gene83521:CDS
ACCACAAGCGCCGGAGTGGG+AGG 0.564951 2.2:+325401 MS.gene83521:CDS
CCCACCACAAGCGCCGGAGT+GGG 0.568859 2.2:+325398 MS.gene83521:CDS
GTTGGTGTCAATCTCGCTGA+GGG 0.574343 2.2:-324868 None:intergenic
GACAATCCTGCAGCTAGGGA+GGG 0.574505 2.2:+326303 MS.gene83521:CDS
CCAAACATATGCAAAAGGCC+TGG 0.577415 2.2:-326469 None:intergenic
AGCTTTGTTGCATAGTGTAG+TGG 0.581324 2.2:+326507 MS.gene83521:CDS
GCCCACCACAAGCGCCGGAG+TGG 0.582189 2.2:+325397 MS.gene83521:CDS
ACAGTTACCGCTAACCTCAC+CGG 0.586784 2.2:+325499 MS.gene83521:CDS
AGGAGCAGCAGCTTCAGAGT+CGG 0.586966 2.2:-325093 None:intergenic
ATTTGAAAACATACGACCAA+GGG 0.596408 2.2:-324844 None:intergenic
GTTAAGGAACCAACTCCTCA+GGG 0.602181 2.2:+325200 MS.gene83521:CDS
CATTGCCTATAGATATTGGA+AGG 0.608050 2.2:+325162 MS.gene83521:CDS
TGAAGTGAGACCTCCCACTC+CGG 0.610091 2.2:-325411 None:intergenic
ACAGTAGCTCAGAATCTCAA+AGG 0.612108 2.2:+325433 MS.gene83521:CDS
TAGCTGCAGCAACAAGAACA+AGG 0.612300 2.2:+324911 MS.gene83521:CDS
AAGGCCTGGTAACCAGTCAA+AGG 0.614442 2.2:-326455 None:intergenic
TACTCAACAACAGCAAGAGG+TGG 0.614584 2.2:-325580 None:intergenic
CTTCCAATATCTATAGGCAA+TGG 0.616470 2.2:-325161 None:intergenic
ATGAATCTAACTAACCTGGT+TGG 0.618468 2.2:-325765 None:intergenic
GCAGAATAGGAAACTGGCTG+TGG 0.618843 2.2:+325375 MS.gene83521:CDS
ATCCTGCAGCTAGGGAGGGT+AGG 0.625245 2.2:+326307 MS.gene83521:CDS
ATGCAGAACTGTAATGAGGA+AGG 0.628521 2.2:-324999 None:intergenic
TTGACTATAGAGATAAAGGA+AGG 0.630332 2.2:+326117 MS.gene83521:CDS
GCTTACTCAACAACAGCAAG+AGG 0.633778 2.2:-325583 None:intergenic
GGACTGCCAATGAATTGGGA+AGG 0.634626 2.2:+325923 MS.gene83521:CDS
CAAGAGGTGGTTTACTACTG+CGG 0.635758 2.2:-325567 None:intergenic
AGTGCATCAACTATTCTCAA+GGG 0.640841 2.2:+325958 MS.gene83521:CDS
GATATCCCTGATCCCAAATG+TGG 0.643558 2.2:-325531 None:intergenic
CAGCAGCTTCAGAGTCGGAG+TGG 0.644616 2.2:-325088 None:intergenic
TATTGTAGGAAGGTCAGGGA+CGG 0.654971 2.2:+325353 MS.gene83521:intron
TGTGGTATTTCGAACCGGTG+AGG 0.659341 2.2:-325513 None:intergenic
CGTTGGTGTCAATCTCGCTG+AGG 0.660418 2.2:-324869 None:intergenic
TTGACACCAACGTTCAGCTG+AGG 0.660689 2.2:+324880 MS.gene83521:CDS
TTGCATGCAGAACTGTAATG+AGG 0.667955 2.2:-325003 None:intergenic
GATTTGAAAACATACGACCA+AGG 0.673412 2.2:-324845 None:intergenic
AAGGGATCCACTCTATAACA+AGG 0.674607 2.2:+325976 MS.gene83521:CDS
ACTATGCCTCAGCTGAACGT+TGG 0.687057 2.2:-324886 None:intergenic
AGAGGTGGTTTACTACTGCG+GGG 0.697959 2.2:-325565 None:intergenic
TTGGTGTCAATCTCGCTGAG+GGG 0.726946 2.2:-324867 None:intergenic
GTTGGGTAAAGTAGACCAGG+AGG 0.744497 2.2:-325120 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATATTATTATTATTATTGT+AGG + chr2.2:325339-325358 MS.gene83521:intron 10.0%
!! TTATTACTAACAATAAAATT+GGG + chr2.2:325878-325897 MS.gene83521:intron 10.0%
!! TTTATTACTAACAATAAAAT+TGG + chr2.2:325877-325896 MS.gene83521:intron 10.0%
!! ATATAATACATTACATACAT+AGG - chr2.2:325258-325277 None:intergenic 15.0%
!! TATTACTAACAATAAAATTG+GGG + chr2.2:325879-325898 MS.gene83521:intron 15.0%
!! TTAATGAAAATCAATATCAA+TGG - chr2.2:326054-326073 None:intergenic 15.0%
!! TTATTATTATTATTGTAGGA+AGG + chr2.2:325343-325362 MS.gene83521:intron 15.0%
!! ATACATAGAATAGAATAGAA+TGG - chr2.2:325290-325309 None:intergenic 20.0%
!! ATTACTAACAATAAAATTGG+GGG + chr2.2:325880-325899 MS.gene83521:intron 20.0%
!!! AGGAAAATCTAATTTTGTTA+TGG + chr2.2:326327-326346 MS.gene83521:CDS 20.0%
!!! GTTTTTATGTTATAACAACT+CGG + chr2.2:326221-326240 MS.gene83521:intron 20.0%
! GTATAAAGAGAGAAGAAATT+GGG - chr2.2:325236-325255 None:intergenic 25.0%
! TTATAACATAAAAACCCTTC+CGG - chr2.2:326216-326235 None:intergenic 25.0%
!! AAAGACATAGATTGATGATT+GGG - chr2.2:325737-325756 None:intergenic 25.0%
AATGAATTGGGAAGGAAAAA+TGG + chr2.2:325931-325950 MS.gene83521:CDS 30.0%
ATCATCATCATCATCAGAAT+TGG - chr2.2:325467-325486 None:intergenic 30.0%
ATTATTATTGTAGGAAGGTC+AGG + chr2.2:325348-325367 MS.gene83521:intron 30.0%
ATTTGAAAACATACGACCAA+GGG - chr2.2:324847-324866 None:intergenic 30.0%
GAAAATCAATATCAATGGCT+TGG - chr2.2:326049-326068 None:intergenic 30.0%
GAAAATGAATCTAACTAACC+TGG - chr2.2:325772-325791 None:intergenic 30.0%
GGTATAAAGAGAGAAGAAAT+TGG - chr2.2:325237-325256 None:intergenic 30.0%
TATAACATAAAAACCCTTCC+GGG - chr2.2:326215-326234 None:intergenic 30.0%
TCTACATATCAAAGACTTGT+TGG - chr2.2:325141-325160 None:intergenic 30.0%
TTATTATTGTAGGAAGGTCA+GGG + chr2.2:325349-325368 MS.gene83521:intron 30.0%
TTGACTATAGAGATAAAGGA+AGG + chr2.2:326117-326136 MS.gene83521:CDS 30.0%
! ATTTTTCCTTCCCAATTCAT+TGG - chr2.2:325932-325951 None:intergenic 30.0%
! CTACATATCAAAGACTTGTT+GGG - chr2.2:325140-325159 None:intergenic 30.0%
! GAAAGACATAGATTGATGAT+TGG - chr2.2:325738-325757 None:intergenic 30.0%
! GTGTACTTGTGTTATTGTTA+AGG + chr2.2:325184-325203 MS.gene83521:CDS 30.0%
! TAACTAGTACATCATTTTGC+AGG + chr2.2:325654-325673 MS.gene83521:intron 30.0%
! TTCCAGCTCTTTGAAAATTT+GGG + chr2.2:325064-325083 MS.gene83521:CDS 30.0%
! TTTCCAGCTCTTTGAAAATT+TGG + chr2.2:325063-325082 MS.gene83521:CDS 30.0%
! TTTGCTATGATTAGGAGAAT+GGG + chr2.2:326275-326294 MS.gene83521:intron 30.0%
!! CGTGAGTTGAAAGTTTTTAA+CGG - chr2.2:326165-326184 None:intergenic 30.0%
!! CTAATTTTGTTATGGACTAC+AGG + chr2.2:326335-326354 MS.gene83521:CDS 30.0%
!! GATGATGATGATACTTTTCT+CGG + chr2.2:325475-325494 MS.gene83521:CDS 30.0%
!! GATTTGTGTTTGCTATGATT+AGG + chr2.2:326267-326286 MS.gene83521:intron 30.0%
!! GTTGAAAATGAGTTGGATTT+TGG - chr2.2:324960-324979 None:intergenic 30.0%
!! TTTTGGAAGAGAAACAACAT+TGG - chr2.2:324943-324962 None:intergenic 30.0%
AAAAACCAAAACCAAACCCT+TGG + chr2.2:324828-324847 MS.gene83521:CDS 35.0%
AAACTTTCAACTCACGTTAG+AGG + chr2.2:326169-326188 MS.gene83521:CDS 35.0%
AACAATAAAATTGGGGGTTC+AGG + chr2.2:325886-325905 MS.gene83521:intron 35.0%
AAGTGCATCAACTATTCTCA+AGG + chr2.2:325957-325976 MS.gene83521:CDS 35.0%
AGACCATTGCCTATAGATAT+TGG + chr2.2:325158-325177 MS.gene83521:CDS 35.0%
AGTGCATCAACTATTCTCAA+GGG + chr2.2:325958-325977 MS.gene83521:CDS 35.0%
ATGAATCTAACTAACCTGGT+TGG - chr2.2:325768-325787 None:intergenic 35.0%
CATTGCCTATAGATATTGGA+AGG + chr2.2:325162-325181 MS.gene83521:CDS 35.0%
CTTCCAATATCTATAGGCAA+TGG - chr2.2:325164-325183 None:intergenic 35.0%
GAAGAATCAGAAGAAGGAAT+AGG - chr2.2:325038-325057 None:intergenic 35.0%
GAGCTTGACTATAGAGATAA+AGG + chr2.2:326113-326132 MS.gene83521:CDS 35.0%
GATTTGAAAACATACGACCA+AGG - chr2.2:324848-324867 None:intergenic 35.0%
GTACACCTTCCAATATCTAT+AGG - chr2.2:325170-325189 None:intergenic 35.0%
GTTCAGGTACAACACATTAA+GGG + chr2.2:325902-325921 MS.gene83521:intron 35.0%
TTGCTTTGTACTCTTGATGA+AGG - chr2.2:326090-326109 None:intergenic 35.0%
TTTCAAAGAGCTGGAAAAGT+GGG - chr2.2:325060-325079 None:intergenic 35.0%
! AAATACCACATTTGGGATCA+GGG + chr2.2:325526-325545 MS.gene83521:CDS 35.0%
! ACTCATTTTCAACAAACACG+AGG + chr2.2:324967-324986 MS.gene83521:CDS 35.0%
! CGTGTTTGTTGAAAATGAGT+TGG - chr2.2:324967-324986 None:intergenic 35.0%
! TTTTCAAAGAGCTGGAAAAG+TGG - chr2.2:325061-325080 None:intergenic 35.0%
!! GAAAGTTTTTAACGGATCTC+TGG - chr2.2:326157-326176 None:intergenic 35.0%
!! GTTTGCTATGATTAGGAGAA+TGG + chr2.2:326274-326293 MS.gene83521:intron 35.0%
!!! CTTAATGTGTTTTGAAGAGG+CGG + chr2.2:326389-326408 MS.gene83521:intron 35.0%
!!! GACCTTAATGTGTTTTGAAG+AGG + chr2.2:326386-326405 MS.gene83521:intron 35.0%
!!! TTAATGTGTTTTGAAGAGGC+GGG + chr2.2:326390-326409 MS.gene83521:intron 35.0%
AAAACATACGACCAAGGGTT+TGG - chr2.2:324842-324861 None:intergenic 40.0%
AAAGAAACTTCCCCTAAGTG+AGG - chr2.2:326011-326030 None:intergenic 40.0%
AAGAGAGAAGAAATTGGGAC+CGG - chr2.2:325231-325250 None:intergenic 40.0%
AAGGGATCCACTCTATAACA+AGG + chr2.2:325976-325995 MS.gene83521:CDS 40.0%
AAGTGGGAAGAATCAGAAGA+AGG - chr2.2:325044-325063 None:intergenic 40.0%
ACAGTAGCTCAGAATCTCAA+AGG + chr2.2:325433-325452 MS.gene83521:CDS 40.0%
AGGTGGAGATTGAGAGATTT+AGG - chr2.2:325608-325627 None:intergenic 40.0%
ATCTGCAGATATCCTTTGAC+TGG + chr2.2:326443-326462 MS.gene83521:intron 40.0%
ATGCAGAACTGTAATGAGGA+AGG - chr2.2:325002-325021 None:intergenic 40.0%
ATTGACAGAGGAAATGGTAC+TGG - chr2.2:326425-326444 None:intergenic 40.0%
CCAAATGTGGTATTTCGAAC+CGG - chr2.2:325521-325540 None:intergenic 40.0%
CCTACAATAGCAACATGTAC+AGG + chr2.2:325683-325702 MS.gene83521:CDS 40.0%
CGCCTCTTCAAAACACATTA+AGG - chr2.2:326391-326410 None:intergenic 40.0%
CGGTTCGAAATACCACATTT+GGG + chr2.2:325519-325538 MS.gene83521:CDS 40.0%
CTAGTTAGCAGAGAAGAATG+AGG - chr2.2:325641-325660 None:intergenic 40.0%
GATCCACTCTATAACAAGGT+AGG + chr2.2:325980-325999 MS.gene83521:intron 40.0%
GCAACAAAGCTTTGCATCAA+TGG - chr2.2:326498-326517 None:intergenic 40.0%
GGAAGCCAAACATATGCAAA+AGG - chr2.2:326477-326496 None:intergenic 40.0%
GGTTCAGGTACAACACATTA+AGG + chr2.2:325901-325920 MS.gene83521:intron 40.0%
TAAATCTCTCAATCTCCACC+TGG + chr2.2:325607-325626 MS.gene83521:intron 40.0%
TAAGGGACTGCCAATGAATT+GGG + chr2.2:325919-325938 MS.gene83521:CDS 40.0%
TAGAGGTAACATCATTGACG+CGG + chr2.2:326186-326205 MS.gene83521:intron 40.0%
TATAGAGATAAAGGAAGGCC+AGG + chr2.2:326122-326141 MS.gene83521:CDS 40.0%
TTAAGGGACTGCCAATGAAT+TGG + chr2.2:325918-325937 MS.gene83521:CDS 40.0%
TTGCATGCAGAACTGTAATG+AGG - chr2.2:325006-325025 None:intergenic 40.0%
! ATATCCTTTGACTGGTTACC+AGG + chr2.2:326451-326470 MS.gene83521:CDS 40.0%
! CCTGTACATGTTGCTATTGT+AGG - chr2.2:325686-325705 None:intergenic 40.0%
! GAAATACCACATTTGGGATC+AGG + chr2.2:325525-325544 MS.gene83521:CDS 40.0%
! GTCCCAAATTTTCAAAGAGC+TGG - chr2.2:325069-325088 None:intergenic 40.0%
!!! TAATGTGTTTTGAAGAGGCG+GGG + chr2.2:326391-326410 MS.gene83521:intron 40.0%
AAGAGGTGGTTTACTACTGC+GGG - chr2.2:325569-325588 None:intergenic 45.0%
AGAGGTAACATCATTGACGC+GGG + chr2.2:326187-326206 MS.gene83521:intron 45.0%
CAAGAGGTGGTTTACTACTG+CGG - chr2.2:325570-325589 None:intergenic 45.0%
CCAAACATATGCAAAAGGCC+TGG - chr2.2:326472-326491 None:intergenic 45.0%
CCACTCTATAACAAGGTAGG+AGG + chr2.2:325983-326002 MS.gene83521:intron 45.0%
CTGCAGATTGACAGAGGAAA+TGG - chr2.2:326431-326450 None:intergenic 45.0%
CTTGTTGGGTAAAGTAGACC+AGG - chr2.2:325126-325145 None:intergenic 45.0%
GATATCCCTGATCCCAAATG+TGG - chr2.2:325534-325553 None:intergenic 45.0%
GCTTACTCAACAACAGCAAG+AGG - chr2.2:325586-325605 None:intergenic 45.0%
GGATATCTGCAGATTGACAG+AGG - chr2.2:326437-326456 None:intergenic 45.0%
GTCTTTCAAGAACACCAACC+AGG + chr2.2:325751-325770 MS.gene83521:CDS 45.0%
GTTAAGGAACCAACTCCTCA+GGG + chr2.2:325200-325219 MS.gene83521:CDS 45.0%
GTTAGCAGAGAAGAATGAGG+CGG - chr2.2:325638-325657 None:intergenic 45.0%
TACTCAACAACAGCAAGAGG+TGG - chr2.2:325583-325602 None:intergenic 45.0%
TAGCTGCAGCAACAAGAACA+AGG + chr2.2:324911-324930 MS.gene83521:CDS 45.0%
TATTGTAGGAAGGTCAGGGA+CGG + chr2.2:325353-325372 MS.gene83521:intron 45.0%
TGTTAAGGAACCAACTCCTC+AGG + chr2.2:325199-325218 MS.gene83521:CDS 45.0%
! CCAGGCCTTTTGCATATGTT+TGG + chr2.2:326469-326488 MS.gene83521:CDS 45.0%
! CCGGTTCGAAATACCACATT+TGG + chr2.2:325518-325537 MS.gene83521:CDS 45.0%
! CCTCCTACCTTGTTATAGAG+TGG - chr2.2:325986-326005 None:intergenic 45.0%
!! TACGACCAAGGGTTTGGTTT+TGG - chr2.2:324836-324855 None:intergenic 45.0%
AAAGTAGACCAGGAGGAAGC+AGG - chr2.2:325116-325135 None:intergenic 50.0%
AAGCAGACAATCCTGCAGCT+AGG + chr2.2:326298-326317 MS.gene83521:CDS 50.0%
AAGGCCTGGTAACCAGTCAA+AGG - chr2.2:326458-326477 None:intergenic 50.0%
ACAGTTACCGCTAACCTCAC+CGG + chr2.2:325499-325518 MS.gene83521:CDS 50.0%
ACTATGCCTCAGCTGAACGT+TGG - chr2.2:324889-324908 None:intergenic 50.0%
AGAGGTGGTTTACTACTGCG+GGG - chr2.2:325568-325587 None:intergenic 50.0%
AGCAGACAATCCTGCAGCTA+GGG + chr2.2:326299-326318 MS.gene83521:CDS 50.0%
GATAAAGGAAGGCCAGGACT+TGG + chr2.2:326128-326147 MS.gene83521:CDS 50.0%
GATCTCTGGATTCCAAGTCC+TGG - chr2.2:326143-326162 None:intergenic 50.0%
GCAGAATAGGAAACTGGCTG+TGG + chr2.2:325375-325394 MS.gene83521:CDS 50.0%
GGACTGCCAATGAATTGGGA+AGG + chr2.2:325923-325942 MS.gene83521:CDS 50.0%
GTTGGGTAAAGTAGACCAGG+AGG - chr2.2:325123-325142 None:intergenic 50.0%
GTTTACTACTGCGGGGCTTA+TGG - chr2.2:325561-325580 None:intergenic 50.0%
TGTGGTATTTCGAACCGGTG+AGG - chr2.2:325516-325535 None:intergenic 50.0%
TTGACACCAACGTTCAGCTG+AGG + chr2.2:324880-324899 MS.gene83521:CDS 50.0%
TTTCGAACCGGTGAGGTTAG+CGG - chr2.2:325509-325528 None:intergenic 50.0%
!! GTTGGTGTCAATCTCGCTGA+GGG - chr2.2:324871-324890 None:intergenic 50.0%
!! TTGGTGTCAATCTCGCTGAG+GGG - chr2.2:324870-324889 None:intergenic 50.0%
AAGGTCAGGGACGGCAGAAT+AGG + chr2.2:325362-325381 MS.gene83521:intron 55.0%
ACATCATTGACGCGGGAACC+CGG + chr2.2:326194-326213 MS.gene83521:intron 55.0%
AGACAATCCTGCAGCTAGGG+AGG + chr2.2:326302-326321 MS.gene83521:CDS 55.0%
AGGAGCAGCAGCTTCAGAGT+CGG - chr2.2:325096-325115 None:intergenic 55.0%
AGGTAGGAGGAGCCTCACTT+AGG + chr2.2:325996-326015 MS.gene83521:intron 55.0%
GACAATCCTGCAGCTAGGGA+GGG + chr2.2:326303-326322 MS.gene83521:CDS 55.0%
GGGACGGCAGAATAGGAAAC+TGG + chr2.2:325369-325388 MS.gene83521:CDS 55.0%
GGTAGGAGGAGCCTCACTTA+GGG + chr2.2:325997-326016 MS.gene83521:intron 55.0%
GTAGGAGGAGCCTCACTTAG+GGG + chr2.2:325998-326017 MS.gene83521:intron 55.0%
TGAAGTGAGACCTCCCACTC+CGG - chr2.2:325414-325433 None:intergenic 55.0%
!! CGTTGGTGTCAATCTCGCTG+AGG - chr2.2:324872-324891 None:intergenic 55.0%
ATCCTGCAGCTAGGGAGGGT+AGG + chr2.2:326307-326326 MS.gene83521:CDS 60.0%
ATTGACGCGGGAACCCGGAA+GGG + chr2.2:326199-326218 MS.gene83521:intron 60.0%
CAGAGAAGAATGAGGCGGCC+AGG - chr2.2:325633-325652 None:intergenic 60.0%
CAGCAGCTTCAGAGTCGGAG+TGG - chr2.2:325091-325110 None:intergenic 60.0%
CCAACTCCTCAGGGTCTCTC+CGG + chr2.2:325209-325228 MS.gene83521:intron 60.0%
CTCTCAATCTCCACCTGGCC+TGG + chr2.2:325612-325631 MS.gene83521:intron 60.0%
TTCCTACCCTCCCTAGCTGC+AGG - chr2.2:326312-326331 None:intergenic 60.0%
AAGAATGAGGCGGCCAGGCC+AGG - chr2.2:325628-325647 None:intergenic 65.0%
CATTGACGCGGGAACCCGGA+AGG + chr2.2:326198-326217 MS.gene83521:intron 65.0%
CCGGAGAGACCCTGAGGAGT+TGG - chr2.2:325212-325231 None:intergenic 65.0%
CTGCTGCTCCTGCTTCCTCC+TGG + chr2.2:325105-325124 MS.gene83521:CDS 65.0%
TTGGGACCGGAGAGACCCTG+AGG - chr2.2:325218-325237 None:intergenic 65.0%
AATGAGGCGGCCAGGCCAGG+TGG - chr2.2:325625-325644 None:intergenic 70.0%
ACCTCCCACTCCGGCGCTTG+TGG - chr2.2:325405-325424 None:intergenic 70.0%
CCCACTCCGGCGCTTGTGGT+GGG - chr2.2:325401-325420 None:intergenic 70.0%
CTGTGGCCCACCACAAGCGC+CGG + chr2.2:325392-325411 MS.gene83521:CDS 70.0%
TCCCACTCCGGCGCTTGTGG+TGG - chr2.2:325402-325421 None:intergenic 70.0%
!! ACCACAAGCGCCGGAGTGGG+AGG + chr2.2:325401-325420 MS.gene83521:CDS 70.0%
!! CCCACCACAAGCGCCGGAGT+GGG + chr2.2:325398-325417 MS.gene83521:CDS 70.0%
!! GCCCACCACAAGCGCCGGAG+TGG + chr2.2:325397-325416 MS.gene83521:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 324822 326526 324822 ID=MS.gene83521
chr2.2 mRNA 324822 326526 324822 ID=MS.gene83521.t1;Parent=MS.gene83521
chr2.2 exon 324822 325221 324822 ID=MS.gene83521.t1.exon1;Parent=MS.gene83521.t1
chr2.2 CDS 324822 325221 324822 ID=cds.MS.gene83521.t1;Parent=MS.gene83521.t1
chr2.2 exon 325365 325599 325365 ID=MS.gene83521.t1.exon2;Parent=MS.gene83521.t1
chr2.2 CDS 325365 325599 325365 ID=cds.MS.gene83521.t1;Parent=MS.gene83521.t1
chr2.2 exon 325676 325772 325676 ID=MS.gene83521.t1.exon3;Parent=MS.gene83521.t1
chr2.2 CDS 325676 325772 325676 ID=cds.MS.gene83521.t1;Parent=MS.gene83521.t1
chr2.2 exon 325908 325997 325908 ID=MS.gene83521.t1.exon4;Parent=MS.gene83521.t1
chr2.2 CDS 325908 325997 325908 ID=cds.MS.gene83521.t1;Parent=MS.gene83521.t1
chr2.2 exon 326098 326190 326098 ID=MS.gene83521.t1.exon5;Parent=MS.gene83521.t1
chr2.2 CDS 326098 326190 326098 ID=cds.MS.gene83521.t1;Parent=MS.gene83521.t1
chr2.2 exon 326289 326356 326289 ID=MS.gene83521.t1.exon6;Parent=MS.gene83521.t1
chr2.2 CDS 326289 326356 326289 ID=cds.MS.gene83521.t1;Parent=MS.gene83521.t1
chr2.2 exon 326451 326526 326451 ID=MS.gene83521.t1.exon7;Parent=MS.gene83521.t1
chr2.2 CDS 326451 326526 326451 ID=cds.MS.gene83521.t1;Parent=MS.gene83521.t1
Gene Sequence

>MS.gene83521

ATGAAGAAAAACCAAAACCAAACCCTTGGTCGTATGTTTTCAAATCCCCTCAGCGAGATTGACACCAACGTTCAGCTGAGGCATAGTCGTAGCTGCAGCAACAAGAACAAGGAAAATGCCAATGTTGTTTCTCTTCCAAAATCCAACTCATTTTCAACAAACACGAGGATTCTTAAGCCTTCCTCATTACAGTTCTGCATGCAAATCAACGATCCTATTCCTTCTTCTGATTCTTCCCACTTTTCCAGCTCTTTGAAAATTTGGGACCACTCCGACTCTGAAGCTGCTGCTCCTGCTTCCTCCTGGTCTACTTTACCCAACAAGTCTTTGATATGTAGACCATTGCCTATAGATATTGGAAGGTGTACTTGTGTTATTGTTAAGGAACCAACTCCTCAGGGTCTCTCCGGTCCCAATTTCTTCTCTCTTTATACCTATGTATGTAATGTATTATATTATTCACTACCATTCTATTCTATTCTATGTATAATATAATATCACTTTGCTATTTAATCAACATATTATTATTATTATTGTAGGAAGGTCAGGGACGGCAGAATAGGAAACTGGCTGTGGCCCACCACAAGCGCCGGAGTGGGAGGTCTCACTTCACAGTAGCTCAGAATCTCAAAGGACTACTCTCCAATTCTGATGATGATGATGATACTTTTCTCGGTACAGTTACCGCTAACCTCACCGGTTCGAAATACCACATTTGGGATCAGGGATATCATCGCCATAAGCCCCGCAGTAGTAAACCACCTCTTGCTGTTGTTGAGTAAGCCTAAATCTCTCAATCTCCACCTGGCCTGGCCGCCTCATTCTTCTCTGCTAACTAGTACATCATTTTGCAGGTATGTACCTACAATAGCAACATGTACAGGAACTCACAGAAGCATCAAAGCTTATATACCCAATCATCAATCTATGTCTTTCAAGAACACCAACCAGGTTAGTTAGATTCATTTTCAATAATATCATGAATCATGATCACATCTACTGTGTCGTCAGTCTGCATACTCACATTGTTTACAGTTCAATTACAACAATATCTCTTTATTACTAACAATAAAATTGGGGGTTCAGGTACAACACATTAAGGGACTGCCAATGAATTGGGAAGGAAAAATGGACAAAGTGCATCAACTATTCTCAAGGGATCCACTCTATAACAAGGTAGGAGGAGCCTCACTTAGGGGAAGTTTCTTTGTCATTTATAGCTAGCCAAGCCATTGATATTGATTTTCATTAATTTGTATTGTCTTCCTTCATCAAGAGTACAAAGCAATTTGAGCTTGACTATAGAGATAAAGGAAGGCCAGGACTTGGAATCCAGAGATCCGTTAAAAACTTTCAACTCACGTTAGAGGTAACATCATTGACGCGGGAACCCGGAAGGGTTTTTATGTTATAACAACTCGGTTAGTTATGTTGTCTACTTAGCTGATTTGTGTTTGCTATGATTAGGAGAATGGGAAGCAGACAATCCTGCAGCTAGGGAGGGTAGGAAAATCTAATTTTGTTATGGACTACAGGTATCAATTCAAATTTTTCTTGTTTTATTGACCTTAATGTGTTTTGAAGAGGCGGGGTTTCATAGCCAGTACCATTTCCTCTGTCAATCTGCAGATATCCTTTGACTGGTTACCAGGCCTTTTGCATATGTTTGGCTTCCATTGATGCAAAGCTTTGTTGCATAGTGTAG
Protein sequence

>MS.gene83521.t1

MKKNQNQTLGRMFSNPLSEIDTNVQLRHSRSCSNKNKENANVVSLPKSNSFSTNTRILKPSSLQFCMQINDPIPSSDSSHFSSSLKIWDHSDSEAAAPASSWSTLPNKSLICRPLPIDIGRCTCVIVKEPTPQGQGRQNRKLAVAHHKRRSGRSHFTVAQNLKGLLSNSDDDDDTFLGTVTANLTGSKYHIWDQGYHRHKPRSSKPPLAVVEYVPTIATCTGTHRSIKAYIPNHQSMSFKNTNQVQHIKGLPMNWEGKMDKVHQLFSRDPLYNKSTKQFELDYRDKGRPGLGIQRSVKNFQLTLEENGKQTILQLGRVGKSNFVMDYRYPLTGYQAFCICLASIDAKLCCIV