Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84225.t1 | XP_003623274.1 | 86.9 | 130 | 7 | 2 | 1 | 126 | 1 | 124 | 4.40E-50 | 207.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84225.t1 | G7KRF0 | 86.9 | 130 | 7 | 2 | 1 | 126 | 1 | 124 | 3.2e-50 | 207.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene84225 | 0.819045 | 1.43E-52 | -1.69E-46 |
| MS.gene050096 | MS.gene84225 | 0.807244 | 5.56E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene84225 | 0.80591 | 1.06E-49 | -1.69E-46 |
| MS.gene05240 | MS.gene84225 | 0.809105 | 2.23E-50 | -1.69E-46 |
| MS.gene053030 | MS.gene84225 | 0.83153 | 1.58E-55 | -1.69E-46 |
| MS.gene053031 | MS.gene84225 | 0.853086 | 3.01E-61 | -1.69E-46 |
| MS.gene053033 | MS.gene84225 | 0.827866 | 1.23E-54 | -1.69E-46 |
| MS.gene053420 | MS.gene84225 | 0.804087 | 2.56E-49 | -1.69E-46 |
| MS.gene054146 | MS.gene84225 | 0.809672 | 1.69E-50 | -1.69E-46 |
| MS.gene054181 | MS.gene84225 | 0.803322 | 3.68E-49 | -1.69E-46 |
| MS.gene055240 | MS.gene84225 | 0.810679 | 1.02E-50 | -1.69E-46 |
| MS.gene057261 | MS.gene84225 | 0.802395 | 5.72E-49 | -1.69E-46 |
| MS.gene057547 | MS.gene84225 | 0.824693 | 7.03E-54 | -1.69E-46 |
| MS.gene058603 | MS.gene84225 | 0.808758 | 2.65E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84225.t1 | MTR_7g068050 | 85.470 | 117 | 7 | 2 | 1 | 113 | 1 | 111 | 6.31e-66 | 196 |
| MS.gene84225.t1 | MTR_7g067950 | 51.961 | 102 | 43 | 3 | 30 | 126 | 27 | 127 | 5.32e-22 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTGAATCGTCAGCAATTC+GGG | 0.244167 | 7.1:-33758869 | MS.gene84225:CDS |
| ACCCGAATTGCTGACGATTC+AGG | 0.254329 | 7.1:+33758868 | None:intergenic |
| TAAGAGGGGATTCTCTTATA+AGG | 0.293421 | 7.1:-33760394 | MS.gene84225:CDS |
| GGAGATCGCGGTTTCTTGAT+TGG | 0.347991 | 7.1:+33760272 | None:intergenic |
| ACCCTGAATCGTCAGCAATT+CGG | 0.349585 | 7.1:-33758870 | MS.gene84225:CDS |
| CCCGAATTGCTGACGATTCA+GGG | 0.374699 | 7.1:+33758869 | None:intergenic |
| ATAACTTGGAAAGCAATTTC+AGG | 0.384541 | 7.1:+33758716 | None:intergenic |
| CGCGGTTTCTTGATTGGAGG+GGG | 0.395599 | 7.1:+33760278 | None:intergenic |
| GGAGAACTCCTTCGCAAACT+TGG | 0.406549 | 7.1:-33758739 | MS.gene84225:CDS |
| GTCAGCAATTCGGGTAAGTC+TGG | 0.408786 | 7.1:-33758860 | MS.gene84225:CDS |
| TAGATATCAGTTTCATAACT+TGG | 0.426133 | 7.1:+33758702 | None:intergenic |
| AAGAGGGGATTCTCTTATAA+GGG | 0.477382 | 7.1:-33760393 | MS.gene84225:CDS |
| ATCTCCGATCTTACAAATCA+AGG | 0.478783 | 7.1:-33760255 | MS.gene84225:CDS |
| TAAGGGAAATAGTTATCCTT+CGG | 0.485583 | 7.1:-33760376 | MS.gene84225:CDS |
| TCTTCCATCGCGTTGTCTGT+TGG | 0.511505 | 7.1:+33758796 | None:intergenic |
| ATCGCGGTTTCTTGATTGGA+GGG | 0.519025 | 7.1:+33760276 | None:intergenic |
| TGATCCTTGATTTGTAAGAT+CGG | 0.527760 | 7.1:+33760251 | None:intergenic |
| GGTAAGTCTGGAGGTGAACA+AGG | 0.534232 | 7.1:-33758848 | MS.gene84225:CDS |
| TACAATGAGTTGCACAAACA+CGG | 0.536112 | 7.1:-33760324 | MS.gene84225:CDS |
| GATCGCGGTTTCTTGATTGG+AGG | 0.552553 | 7.1:+33760275 | None:intergenic |
| ATCAAGTAAGTTATGGGTGA+AGG | 0.558905 | 7.1:-33760423 | None:intergenic |
| TGGGTGAAGGCGATCATAAG+AGG | 0.561952 | 7.1:-33760410 | MS.gene84225:CDS |
| GCAGCTGTGTTGGTTTCTCA+AGG | 0.564887 | 7.1:-33758760 | MS.gene84225:CDS |
| TGCACACACCGCAGCTGTGT+TGG | 0.571415 | 7.1:-33758770 | MS.gene84225:CDS |
| GGGTGAAGGCGATCATAAGA+GGG | 0.583008 | 7.1:-33760409 | MS.gene84225:CDS |
| ATTTGTAAGATCGGAGATCG+CGG | 0.611829 | 7.1:+33760260 | None:intergenic |
| TCGCGGTTTCTTGATTGGAG+GGG | 0.613086 | 7.1:+33760277 | None:intergenic |
| TATCCTTCGGATGATGAGCA+TGG | 0.617244 | 7.1:-33760363 | MS.gene84225:CDS |
| ACAATGAGTTGCACAAACAC+GGG | 0.619558 | 7.1:-33760323 | MS.gene84225:CDS |
| ATTTCAGGCCAAGTTTGCGA+AGG | 0.621581 | 7.1:+33758731 | None:intergenic |
| ACTCCATGCTCATCATCCGA+AGG | 0.646422 | 7.1:+33760360 | None:intergenic |
| GCGGTTTCTTGATTGGAGGG+GGG | 0.647692 | 7.1:+33760279 | None:intergenic |
| TTGAGAAACCAACACAGCTG+CGG | 0.651919 | 7.1:+33758762 | None:intergenic |
| TGTTCCAACAGACAACGCGA+TGG | 0.657088 | 7.1:-33758800 | MS.gene84225:CDS |
| AGCAATTCGGGTAAGTCTGG+AGG | 0.657891 | 7.1:-33758857 | MS.gene84225:CDS |
| GTAAGTCTGGAGGTGAACAA+GGG | 0.696458 | 7.1:-33758847 | MS.gene84225:CDS |
| GGTGAAGGCGATCATAAGAG+GGG | 0.710139 | 7.1:-33760408 | MS.gene84225:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAAATTAATAATATTTTA+AGG | + | chr7.1:33759690-33759709 | None:intergenic | 0.0% |
| !!! | TTATTTTTTTTAATAAAAAT+TGG | - | chr7.1:33759617-33759636 | MS.gene84225:intron | 0.0% |
| !! | AAAAATTGATATATTTAGAT+GGG | + | chr7.1:33759524-33759543 | None:intergenic | 10.0% |
| !! | GATAAATTAATAAAGATTAA+AGG | - | chr7.1:33759748-33759767 | MS.gene84225:intron | 10.0% |
| !! | TAAAAATTGATATATTTAGA+TGG | + | chr7.1:33759525-33759544 | None:intergenic | 10.0% |
| !!! | AAAAATAGATAAAATGTTTT+AGG | + | chr7.1:33760025-33760044 | None:intergenic | 10.0% |
| !!! | TAAAAATTTCATTCAAATTT+TGG | + | chr7.1:33759949-33759968 | None:intergenic | 10.0% |
| !! | AAAAGAACAAACATAAAAAT+TGG | - | chr7.1:33759903-33759922 | MS.gene84225:intron | 15.0% |
| !! | AAAGTAAAATAAATAGTATG+AGG | - | chr7.1:33760050-33760069 | MS.gene84225:intron | 15.0% |
| !! | AACTCTATTTATTATTTGTT+GGG | - | chr7.1:33759482-33759501 | MS.gene84225:intron | 15.0% |
| !!! | AAACTAAAACTTTCTTTTTT+TGG | - | chr7.1:33759182-33759201 | MS.gene84225:intron | 15.0% |
| !!! | ACTATAAATTTGTTTCTTTA+AGG | - | chr7.1:33759383-33759402 | MS.gene84225:intron | 15.0% |
| !!! | ATTTCCCATATTTTTTTTAA+GGG | + | chr7.1:33759058-33759077 | None:intergenic | 15.0% |
| !!! | CTAAATAATTCTTTGTATTT+TGG | - | chr7.1:33759557-33759576 | MS.gene84225:intron | 15.0% |
| !!! | TAATCTTTAATTTGAAGTTA+TGG | - | chr7.1:33759726-33759745 | MS.gene84225:intron | 15.0% |
| !! | AAATTAAAGATGGAAATGAA+TGG | + | chr7.1:33758987-33759006 | None:intergenic | 20.0% |
| !! | AGATCCCTTAAAAAAAATAT+GGG | - | chr7.1:33759051-33759070 | MS.gene84225:intron | 20.0% |
| !! | ATATCAGAAAATAAGTCATT+TGG | - | chr7.1:33759858-33759877 | MS.gene84225:intron | 20.0% |
| !! | ATTAGTAACCGAAAATTTAT+AGG | + | chr7.1:33759584-33759603 | None:intergenic | 20.0% |
| !! | CAAACATAAAAATTGGTAAA+AGG | - | chr7.1:33759910-33759929 | MS.gene84225:intron | 20.0% |
| !! | CAACTCTATTTATTATTTGT+TGG | - | chr7.1:33759481-33759500 | MS.gene84225:intron | 20.0% |
| !! | CATATATAGTAATAATGCAT+TGG | - | chr7.1:33759113-33759132 | MS.gene84225:intron | 20.0% |
| !! | TAATCCATTGAAATTAAAGA+TGG | + | chr7.1:33758997-33759016 | None:intergenic | 20.0% |
| !! | TAGATCCCTTAAAAAAAATA+TGG | - | chr7.1:33759050-33759069 | MS.gene84225:intron | 20.0% |
| !! | TTCATAGATGTCAAAATTAA+AGG | + | chr7.1:33758932-33758951 | None:intergenic | 20.0% |
| !!! | AAAAAGCGACAAATATTTTT+TGG | + | chr7.1:33759248-33759267 | None:intergenic | 20.0% |
| !!! | AAAAGCGACAAATATTTTTT+GGG | + | chr7.1:33759247-33759266 | None:intergenic | 20.0% |
| !!! | ATTTCCATCTTTAATTTCAA+TGG | - | chr7.1:33758990-33759009 | MS.gene84225:intron | 20.0% |
| !!! | ATTTTGGTCCTATAAATTTT+CGG | - | chr7.1:33759573-33759592 | MS.gene84225:intron | 20.0% |
| !!! | CATTTCCCATATTTTTTTTA+AGG | + | chr7.1:33759059-33759078 | None:intergenic | 20.0% |
| ! | ATCAATAATAACAAGTGAGT+TGG | - | chr7.1:33758893-33758912 | MS.gene84225:intron | 25.0% |
| !! | ATTATTTGTTGGGTTAACTA+TGG | - | chr7.1:33759492-33759511 | MS.gene84225:intron | 25.0% |
| !!! | TTTTAAGGGATCTATGTTAA+AGG | + | chr7.1:33759044-33759063 | None:intergenic | 25.0% |
| ATAACTTGGAAAGCAATTTC+AGG | + | chr7.1:33760412-33760431 | None:intergenic | 30.0% | |
| CATGCAGAAAGTTGAAAATA+GGG | + | chr7.1:33759315-33759334 | None:intergenic | 30.0% | |
| TAAGGGAAATAGTTATCCTT+CGG | - | chr7.1:33758749-33758768 | MS.gene84225:CDS | 30.0% | |
| TCATGCAGAAAGTTGAAAAT+AGG | + | chr7.1:33759316-33759335 | None:intergenic | 30.0% | |
| TTGTGAAGTCAACTCTTTAA+AGG | - | chr7.1:33759139-33759158 | MS.gene84225:intron | 30.0% | |
| !! | TCTTTAAGGTGTATAAGAGA+GGG | - | chr7.1:33759397-33759416 | MS.gene84225:intron | 30.0% |
| !! | TGATCCTTGATTTGTAAGAT+CGG | + | chr7.1:33758877-33758896 | None:intergenic | 30.0% |
| !! | TTCTTTAAGGTGTATAAGAG+AGG | - | chr7.1:33759396-33759415 | MS.gene84225:intron | 30.0% |
| !!! | ATTTTTTGGGTTGTGTTAAC+CGG | + | chr7.1:33759234-33759253 | None:intergenic | 30.0% |
| AAGAGGGGATTCTCTTATAA+GGG | - | chr7.1:33758732-33758751 | MS.gene84225:CDS | 35.0% | |
| ATCTCCGATCTTACAAATCA+AGG | - | chr7.1:33758870-33758889 | MS.gene84225:CDS | 35.0% | |
| TAAGAGGGGATTCTCTTATA+AGG | - | chr7.1:33758731-33758750 | MS.gene84225:CDS | 35.0% | |
| TACAATGAGTTGCACAAACA+CGG | - | chr7.1:33758801-33758820 | MS.gene84225:CDS | 35.0% | |
| TTTAGTCAGTGAGCTTAACT+CGG | + | chr7.1:33759456-33759475 | None:intergenic | 35.0% | |
| TTTCAACTTTCTGCATGAGT+CGG | - | chr7.1:33759318-33759337 | MS.gene84225:intron | 35.0% | |
| ACAATGAGTTGCACAAACAC+GGG | - | chr7.1:33758802-33758821 | MS.gene84225:CDS | 40.0% | |
| ATTTGTAAGATCGGAGATCG+CGG | + | chr7.1:33758868-33758887 | None:intergenic | 40.0% | |
| ACCCTGAATCGTCAGCAATT+CGG | - | chr7.1:33760255-33760274 | MS.gene84225:CDS | 45.0% | |
| ATCGCGGTTTCTTGATTGGA+GGG | + | chr7.1:33758852-33758871 | None:intergenic | 45.0% | |
| ATTTCAGGCCAAGTTTGCGA+AGG | + | chr7.1:33760397-33760416 | None:intergenic | 45.0% | |
| GTAAGTCTGGAGGTGAACAA+GGG | - | chr7.1:33760278-33760297 | MS.gene84225:CDS | 45.0% | |
| TATCCTTCGGATGATGAGCA+TGG | - | chr7.1:33758762-33758781 | MS.gene84225:CDS | 45.0% | |
| TTGAGAAACCAACACAGCTG+CGG | + | chr7.1:33760366-33760385 | None:intergenic | 45.0% | |
| ! | TCGGTTGGTATGCATGAGTT+GGG | + | chr7.1:33759437-33759456 | None:intergenic | 45.0% |
| ! | TTTTCTTAACCAGTGCCCCA+GGG | - | chr7.1:33759205-33759224 | MS.gene84225:intron | 45.0% |
| AAATGTTAACCAGTGCCCCC+GGG | - | chr7.1:33759074-33759093 | MS.gene84225:intron | 50.0% | |
| ACCCGAATTGCTGACGATTC+AGG | + | chr7.1:33760260-33760279 | None:intergenic | 50.0% | |
| ACTCCATGCTCATCATCCGA+AGG | + | chr7.1:33758768-33758787 | None:intergenic | 50.0% | |
| AGCAATTCGGGTAAGTCTGG+AGG | - | chr7.1:33760268-33760287 | MS.gene84225:CDS | 50.0% | |
| CCCGAATTGCTGACGATTCA+GGG | + | chr7.1:33760259-33760278 | None:intergenic | 50.0% | |
| CCCTGAATCGTCAGCAATTC+GGG | - | chr7.1:33760256-33760275 | MS.gene84225:CDS | 50.0% | |
| GAAATGTTAACCAGTGCCCC+CGG | - | chr7.1:33759073-33759092 | MS.gene84225:intron | 50.0% | |
| GATCGCGGTTTCTTGATTGG+AGG | + | chr7.1:33758853-33758872 | None:intergenic | 50.0% | |
| GGAGAACTCCTTCGCAAACT+TGG | - | chr7.1:33760386-33760405 | MS.gene84225:CDS | 50.0% | |
| GGAGATCGCGGTTTCTTGAT+TGG | + | chr7.1:33758856-33758875 | None:intergenic | 50.0% | |
| GGGTGAAGGCGATCATAAGA+GGG | - | chr7.1:33758716-33758735 | MS.gene84225:CDS | 50.0% | |
| GGTAAGTCTGGAGGTGAACA+AGG | - | chr7.1:33760277-33760296 | MS.gene84225:CDS | 50.0% | |
| GGTGAAGGCGATCATAAGAG+GGG | - | chr7.1:33758717-33758736 | MS.gene84225:CDS | 50.0% | |
| GTCAGCAATTCGGGTAAGTC+TGG | - | chr7.1:33760265-33760284 | MS.gene84225:CDS | 50.0% | |
| GTCAGTGAGCTTAACTCGGT+TGG | + | chr7.1:33759452-33759471 | None:intergenic | 50.0% | |
| TCGCGGTTTCTTGATTGGAG+GGG | + | chr7.1:33758851-33758870 | None:intergenic | 50.0% | |
| TCTTCCATCGCGTTGTCTGT+TGG | + | chr7.1:33760332-33760351 | None:intergenic | 50.0% | |
| TGGGTGAAGGCGATCATAAG+AGG | - | chr7.1:33758715-33758734 | MS.gene84225:CDS | 50.0% | |
| TGTTCCAACAGACAACGCGA+TGG | - | chr7.1:33760325-33760344 | MS.gene84225:CDS | 50.0% | |
| TTGATTGGAGGGGGGAGAAT+TGG | + | chr7.1:33758841-33758860 | None:intergenic | 50.0% | |
| TTTCTTAACCAGTGCCCCAG+GGG | - | chr7.1:33759206-33759225 | MS.gene84225:intron | 50.0% | |
| ! | CGGTTGGTATGCATGAGTTG+GGG | + | chr7.1:33759436-33759455 | None:intergenic | 50.0% |
| ! | CTCGGTTGGTATGCATGAGT+TGG | + | chr7.1:33759438-33759457 | None:intergenic | 50.0% |
| ! | GTTTTCTTAACCAGTGCCCC+AGG | - | chr7.1:33759204-33759223 | MS.gene84225:intron | 50.0% |
| !! | GCAGCTGTGTTGGTTTCTCA+AGG | - | chr7.1:33760365-33760384 | MS.gene84225:CDS | 50.0% |
| AATGTTAACCAGTGCCCCCG+GGG | - | chr7.1:33759075-33759094 | MS.gene84225:intron | 55.0% | |
| CGCGGTTTCTTGATTGGAGG+GGG | + | chr7.1:33758850-33758869 | None:intergenic | 55.0% | |
| GAGGGGGGAGAATTGGATTC+TGG | + | chr7.1:33758834-33758853 | None:intergenic | 55.0% | |
| GCGGTTTCTTGATTGGAGGG+GGG | + | chr7.1:33758849-33758868 | None:intergenic | 55.0% | |
| TGCTTCCTTAACCAGTGCCC+CGG | + | chr7.1:33759095-33759114 | None:intergenic | 55.0% | |
| TTGTGTTAACCGGTGCCCCT+GGG | + | chr7.1:33759224-33759243 | None:intergenic | 55.0% | |
| CTTCCTTAACCAGTGCCCCG+GGG | + | chr7.1:33759093-33759112 | None:intergenic | 60.0% | |
| GCTTCCTTAACCAGTGCCCC+GGG | + | chr7.1:33759094-33759113 | None:intergenic | 60.0% | |
| GTTGTGTTAACCGGTGCCCC+TGG | + | chr7.1:33759225-33759244 | None:intergenic | 60.0% | |
| TGCACACACCGCAGCTGTGT+TGG | - | chr7.1:33760355-33760374 | MS.gene84225:CDS | 60.0% | |
| TGTGTTAACCGGTGCCCCTG+GGG | + | chr7.1:33759223-33759242 | None:intergenic | 60.0% | |
| TTCCTTAACCAGTGCCCCGG+GGG | + | chr7.1:33759092-33759111 | None:intergenic | 60.0% | |
| ! | AACCAGTGCCCCAGGGGCAC+CGG | - | chr7.1:33759212-33759231 | MS.gene84225:intron | 70.0% |
| !! | TGCCCCCGGGGCACTGGTTA+AGG | - | chr7.1:33759087-33759106 | MS.gene84225:intron | 70.0% |
| AACCAGTGCCCCCGGGGCAC+TGG | - | chr7.1:33759081-33759100 | MS.gene84225:intron | 75.0% | |
| AACCGGTGCCCCTGGGGCAC+TGG | + | chr7.1:33759217-33759236 | None:intergenic | 75.0% | |
| ! | AACCAGTGCCCCGGGGGCAC+TGG | + | chr7.1:33759086-33759105 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 33758714 | 33760433 | 33758714 | ID=MS.gene84225 |
| chr7.1 | mRNA | 33758714 | 33760433 | 33758714 | ID=MS.gene84225.t1;Parent=MS.gene84225 |
| chr7.1 | exon | 33760241 | 33760433 | 33760241 | ID=MS.gene84225.t1.exon1;Parent=MS.gene84225.t1 |
| chr7.1 | CDS | 33760241 | 33760433 | 33760241 | ID=cds.MS.gene84225.t1;Parent=MS.gene84225.t1 |
| chr7.1 | exon | 33758714 | 33758901 | 33758714 | ID=MS.gene84225.t1.exon2;Parent=MS.gene84225.t1 |
| chr7.1 | CDS | 33758714 | 33758901 | 33758714 | ID=cds.MS.gene84225.t1;Parent=MS.gene84225.t1 |
| Gene Sequence |
| Protein sequence |
