AlfalfaGEDB | MS.gene84309

M. sativa cultivar XinJiangDaYe / MS.gene84309

KEGG: K12581
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF04857.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84309.t1	XP_024629048.1	62.1	272	95	2	12	283	7	270	1.40E-91	346.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84309.t1	Q9FMS6	54.9	273	113	4	14	283	12	277	2.9e-74	280.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84309.t1	I3SR57	62.1	272	95	2	12	283	7	270	9.9e-92	346.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene065708	MS.gene84309	PPI
MS.gene053225	MS.gene84309	PPI
MS.gene034816	MS.gene84309	PPI
MS.gene72978	MS.gene84309	PPI
MS.gene034815	MS.gene84309	PPI
MS.gene025629	MS.gene84309	PPI
MS.gene21541	MS.gene84309	PPI
MS.gene034250	MS.gene84309	PPI
MS.gene72977	MS.gene84309	PPI
MS.gene025628	MS.gene84309	PPI
MS.gene065709	MS.gene84309	PPI
MS.gene007154	MS.gene84309	PPI
MS.gene000609	MS.gene84309	PPI
MS.gene41100	MS.gene84309	PPI
MS.gene065707	MS.gene84309	PPI
MS.gene41101	MS.gene84309	PPI
MS.gene41097	MS.gene84309	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84309.t1	AT5G22250	54.945	273	113	4	14	283	12	277	3.39e-96	285
MS.gene84309.t1	AT3G44260	52.265	287	118	6	4	283	5	279	9.63e-94	278
MS.gene84309.t1	AT1G15920	45.936	283	145	5	1	282	1	276	3.29e-78	239
MS.gene84309.t1	AT1G15920	45.936	283	145	5	1	282	1	276	3.29e-78	239
MS.gene84309.t1	AT2G32070	47.601	271	125	6	14	281	10	266	1.74e-77	237
MS.gene84309.t1	AT1G80780	46.296	270	129	5	14	281	10	265	6.62e-75	230
MS.gene84309.t1	AT1G80780	46.296	270	129	5	14	281	10	265	6.62e-75	230
MS.gene84309.t1	AT1G80780	46.296	270	129	5	14	281	10	265	1.02e-74	230
MS.gene84309.t1	AT5G10960	48.134	268	127	5	14	281	10	265	1.27e-74	229
MS.gene84309.t1	AT1G61470	34.818	247	136	6	18	260	4	229	1.01e-33	124
MS.gene84309.t1	AT1G06450	34.524	252	136	8	15	259	8	237	1.63e-33	125
MS.gene84309.t1	AT1G27820	34.387	253	143	7	12	260	2	235	2.87e-32	121
MS.gene84309.t1	AT3G44240	34.615	234	132	7	29	260	2	216	4.73e-32	119
MS.gene84309.t1	AT1G27890	34.008	247	140	7	18	260	3	230	1.97e-30	116

Find 59 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGGCAATCTTCCCGATTT+TGG	0.132015	2.2:+12006042	MS.gene84309:CDS
CTCGTATGATTTCGGATATT+TGG	0.195704	2.2:+12006278	MS.gene84309:CDS
AAATATGCTGGAGTGCTCTT+TGG	0.291631	2.2:+12006573	MS.gene84309:CDS
TTTCATAGCTCGTATGATTT+CGG	0.312575	2.2:+12006270	MS.gene84309:CDS
TTAGTTGAAGACGGCTGATT+TGG	0.325349	2.2:-12005769	None:intergenic
CGTCTCTTGACACAATTGTT+TGG	0.329196	2.2:+12006351	MS.gene84309:CDS
GCTGAATTAATGTTCTCTTC+AGG	0.331405	2.2:+12006213	MS.gene84309:CDS
TCAATGTTTACAAAATTTCC+TGG	0.365989	2.2:+12005880	MS.gene84309:CDS
CAAACTTGACGGATCACAAT+AGG	0.371885	2.2:-12005793	None:intergenic
CTATTGTGATCCGTCAAGTT+TGG	0.384439	2.2:+12005794	MS.gene84309:CDS
TTTCAACGACCAAGTTGTTT+GGG	0.390947	2.2:+12006242	MS.gene84309:CDS
CGGTTAATGTAAGGCCGACT+TGG	0.392203	2.2:-12006014	None:intergenic
GGAGTTATCTGTTCCAAAAT+CGG	0.393551	2.2:-12006055	None:intergenic
CAGAGTAGATTAACTGAGTC+TGG	0.394312	2.2:-12006132	None:intergenic
GTTCAAAGTCGATCCCTTGA+TGG	0.401109	2.2:-12006155	None:intergenic
TAATGAGTTGTACGACAGTC+TGG	0.414224	2.2:+12006413	MS.gene84309:CDS
GAACAGATAACTCCTGCATA+TGG	0.414348	2.2:+12006064	MS.gene84309:CDS
CCTTGTCGTCGGTTAATGTA+AGG	0.416041	2.2:-12006023	None:intergenic
TGCTGGAGTGCTCTTTGGAT+TGG	0.446608	2.2:+12006578	MS.gene84309:CDS
AAACAACTTGGTCGTTGAAA+AGG	0.451021	2.2:-12006239	None:intergenic
AGGTATAGTGACATCGACTT+TGG	0.458606	2.2:-12005917	None:intergenic
GTTAATCTACTCTGCCATCA+AGG	0.460897	2.2:+12006141	MS.gene84309:CDS
GAAGTTACCATCTCGCTTAG+AGG	0.462416	2.2:+12006320	MS.gene84309:CDS
ATCAACATCAACAAATATGC+TGG	0.464781	2.2:+12006561	MS.gene84309:CDS
CTGGTTAGCTTTCGATAAGA+TGG	0.469314	2.2:+12006512	MS.gene84309:CDS
GGTATAGTGACATCGACTTT+GGG	0.469620	2.2:-12005916	None:intergenic
CATCTTATCGAAAGCTAACC+AGG	0.475919	2.2:-12006511	None:intergenic
CGAGCAATACTCATATTTGA+AGG	0.482644	2.2:+12005966	MS.gene84309:CDS
ACGAATCCATGTGGCGGAGG+TGG	0.498503	2.2:-12005942	None:intergenic
GCTCGGATAGTTTGTTGACC+TGG	0.498750	2.2:+12006493	MS.gene84309:CDS
AATTGTCAGACCAAACTTGA+CGG	0.499910	2.2:-12005804	None:intergenic
TCCATGTGGCGGAGGTGGTA+AGG	0.503759	2.2:-12005937	None:intergenic
TTTGAACGCAATCGTTTATG+TGG	0.507147	2.2:+12006171	MS.gene84309:CDS
TACAAGTGAGTCGTGCAGTC+GGG	0.512427	2.2:+12006454	MS.gene84309:CDS
GAGTTATCTGTTCCAAAATC+GGG	0.519850	2.2:-12006054	None:intergenic
TTCAGCAAACTGAGAAGAGC+TGG	0.522652	2.2:-12006196	None:intergenic
AACAGATAACTCCTGCATAT+GGG	0.530322	2.2:+12006065	MS.gene84309:CDS
GAAATTGAACTCCCATATGC+AGG	0.536122	2.2:-12006076	None:intergenic
GAAAAGTCACCCAAACAACT+TGG	0.541604	2.2:-12006251	None:intergenic
GGGAAGATTGCCCTTGTCGT+CGG	0.546043	2.2:-12006034	None:intergenic
TGACATCGACTTTGGGTGCA+TGG	0.548830	2.2:-12005909	None:intergenic
GTTGTACGACAGTCTGGAGC+AGG	0.550195	2.2:+12006419	MS.gene84309:CDS
ACCTTACCACCTCCGCCACA+TGG	0.552072	2.2:+12005936	MS.gene84309:CDS
CGTACGAATCCATGTGGCGG+AGG	0.557071	2.2:-12005945	None:intergenic
ACAATAGGTTTAGTTGAAGA+CGG	0.574619	2.2:-12005778	None:intergenic
CCTTACATTAACCGACGACA+AGG	0.579664	2.2:+12006023	MS.gene84309:CDS
GTCGGGAATTCTCATCAAGC+CGG	0.579736	2.2:+12006471	MS.gene84309:CDS
GAATTCTCATCAAGCCGGCT+CGG	0.580731	2.2:+12006476	MS.gene84309:CDS
TTAATCTACTCTGCCATCAA+GGG	0.584976	2.2:+12006142	MS.gene84309:CDS
CTACAAGTGAGTCGTGCAGT+CGG	0.588735	2.2:+12006453	MS.gene84309:CDS
GGTCAACAAACTATCCGAGC+CGG	0.615610	2.2:-12006490	None:intergenic
GATTTGAAACTTATCCAAGT+CGG	0.617501	2.2:+12006000	MS.gene84309:CDS
GAAACTTGCGAAAGATGCTA+CGG	0.634561	2.2:-12005849	None:intergenic
GTACGACAGTCTGGAGCAGG+TGG	0.639741	2.2:+12006422	MS.gene84309:CDS
TTGGGTGCATGGATGACACC+AGG	0.647634	2.2:-12005898	None:intergenic
AGAAACTCCTCTAAGCGAGA+TGG	0.649937	2.2:-12006327	None:intergenic
CTTACATTAACCGACGACAA+GGG	0.672450	2.2:+12006024	MS.gene84309:CDS
GCTCGTACGAATCCATGTGG+CGG	0.707562	2.2:-12005948	None:intergenic
ATTGCTCGTACGAATCCATG+TGG	0.744442	2.2:-12005951	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCAATGTTTACAAAATTTCC+TGG	+	chr2.2:12005880-12005899	MS.gene84309:CDS	25.0%
	ACAATAGGTTTAGTTGAAGA+CGG	-	chr2.2:12005781-12005800	None:intergenic	30.0%
	ATCAACATCAACAAATATGC+TGG	+	chr2.2:12006561-12006580	MS.gene84309:CDS	30.0%
	TTTCATAGCTCGTATGATTT+CGG	+	chr2.2:12006270-12006289	MS.gene84309:CDS	30.0%
!	GATTTGAAACTTATCCAAGT+CGG	+	chr2.2:12006000-12006019	MS.gene84309:CDS	30.0%
!!	ATTTGGTGAAAGCTTTTACT+GGG	+	chr2.2:12006295-12006314	MS.gene84309:CDS	30.0%
!!	TATTTGGTGAAAGCTTTTAC+TGG	+	chr2.2:12006294-12006313	MS.gene84309:CDS	30.0%
	AAACAACTTGGTCGTTGAAA+AGG	-	chr2.2:12006242-12006261	None:intergenic	35.0%
	AACAGATAACTCCTGCATAT+GGG	+	chr2.2:12006065-12006084	MS.gene84309:CDS	35.0%
	AATTGTCAGACCAAACTTGA+CGG	-	chr2.2:12005807-12005826	None:intergenic	35.0%
	CGAGCAATACTCATATTTGA+AGG	+	chr2.2:12005966-12005985	MS.gene84309:CDS	35.0%
	CTCGTATGATTTCGGATATT+TGG	+	chr2.2:12006278-12006297	MS.gene84309:CDS	35.0%
	GAGTTATCTGTTCCAAAATC+GGG	-	chr2.2:12006057-12006076	None:intergenic	35.0%
	GCTGAATTAATGTTCTCTTC+AGG	+	chr2.2:12006213-12006232	MS.gene84309:CDS	35.0%
	GGAGTTATCTGTTCCAAAAT+CGG	-	chr2.2:12006058-12006077	None:intergenic	35.0%
	TTAATCTACTCTGCCATCAA+GGG	+	chr2.2:12006142-12006161	MS.gene84309:CDS	35.0%
	TTTCAACGACCAAGTTGTTT+GGG	+	chr2.2:12006242-12006261	MS.gene84309:CDS	35.0%
	TTTGAACGCAATCGTTTATG+TGG	+	chr2.2:12006171-12006190	MS.gene84309:CDS	35.0%
!	TTTTCAACGACCAAGTTGTT+TGG	+	chr2.2:12006241-12006260	MS.gene84309:CDS	35.0%
	CAGAGTAGATTAACTGAGTC+TGG	-	chr2.2:12006135-12006154	None:intergenic	40.0%
	CATCTTATCGAAAGCTAACC+AGG	-	chr2.2:12006514-12006533	None:intergenic	40.0%
	CGTCTCTTGACACAATTGTT+TGG	+	chr2.2:12006351-12006370	MS.gene84309:CDS	40.0%
	CTATTGTGATCCGTCAAGTT+TGG	+	chr2.2:12005794-12005813	MS.gene84309:CDS	40.0%
	CTTACATTAACCGACGACAA+GGG	+	chr2.2:12006024-12006043	MS.gene84309:CDS	40.0%
	GAAAAGTCACCCAAACAACT+TGG	-	chr2.2:12006254-12006273	None:intergenic	40.0%
	GAAACTTGCGAAAGATGCTA+CGG	-	chr2.2:12005852-12005871	None:intergenic	40.0%
	GAAATTGAACTCCCATATGC+AGG	-	chr2.2:12006079-12006098	None:intergenic	40.0%
	GAACAGATAACTCCTGCATA+TGG	+	chr2.2:12006064-12006083	MS.gene84309:CDS	40.0%
	GTTAATCTACTCTGCCATCA+AGG	+	chr2.2:12006141-12006160	MS.gene84309:CDS	40.0%
	TAATGAGTTGTACGACAGTC+TGG	+	chr2.2:12006413-12006432	MS.gene84309:CDS	40.0%
	TTAGTTGAAGACGGCTGATT+TGG	-	chr2.2:12005772-12005791	None:intergenic	40.0%
!	AAATATGCTGGAGTGCTCTT+TGG	+	chr2.2:12006573-12006592	MS.gene84309:CDS	40.0%
!	CAAACTTGACGGATCACAAT+AGG	-	chr2.2:12005796-12005815	None:intergenic	40.0%
!!	AGGTATAGTGACATCGACTT+TGG	-	chr2.2:12005920-12005939	None:intergenic	40.0%
!!	CTGGTTAGCTTTCGATAAGA+TGG	+	chr2.2:12006512-12006531	MS.gene84309:CDS	40.0%
!!	GGTATAGTGACATCGACTTT+GGG	-	chr2.2:12005919-12005938	None:intergenic	40.0%
	AGAAACTCCTCTAAGCGAGA+TGG	-	chr2.2:12006330-12006349	None:intergenic	45.0%
	ATTGCTCGTACGAATCCATG+TGG	-	chr2.2:12005954-12005973	None:intergenic	45.0%
	CCTTACATTAACCGACGACA+AGG	+	chr2.2:12006023-12006042	MS.gene84309:CDS	45.0%
	GAAGTTACCATCTCGCTTAG+AGG	+	chr2.2:12006320-12006339	MS.gene84309:CDS	45.0%
	GTTCAAAGTCGATCCCTTGA+TGG	-	chr2.2:12006158-12006177	None:intergenic	45.0%
	TTCAGCAAACTGAGAAGAGC+TGG	-	chr2.2:12006199-12006218	None:intergenic	45.0%
!	AAGGGCAATCTTCCCGATTT+TGG	+	chr2.2:12006042-12006061	MS.gene84309:CDS	45.0%
!	CCTTGTCGTCGGTTAATGTA+AGG	-	chr2.2:12006026-12006045	None:intergenic	45.0%
!	TGGTGAAAGCTTTTACTGGG+AGG	+	chr2.2:12006298-12006317	MS.gene84309:CDS	45.0%
	CGGTTAATGTAAGGCCGACT+TGG	-	chr2.2:12006017-12006036	None:intergenic	50.0%
	CTACAAGTGAGTCGTGCAGT+CGG	+	chr2.2:12006453-12006472	MS.gene84309:CDS	50.0%
	GAATTCTCATCAAGCCGGCT+CGG	+	chr2.2:12006476-12006495	MS.gene84309:CDS	50.0%
	GGTCAACAAACTATCCGAGC+CGG	-	chr2.2:12006493-12006512	None:intergenic	50.0%
	GTCGGGAATTCTCATCAAGC+CGG	+	chr2.2:12006471-12006490	MS.gene84309:CDS	50.0%
	TACAAGTGAGTCGTGCAGTC+GGG	+	chr2.2:12006454-12006473	MS.gene84309:CDS	50.0%
!	TGCTGGAGTGCTCTTTGGAT+TGG	+	chr2.2:12006578-12006597	MS.gene84309:CDS	50.0%
!!	GCTCGGATAGTTTGTTGACC+TGG	+	chr2.2:12006493-12006512	MS.gene84309:CDS	50.0%
!!	TGACATCGACTTTGGGTGCA+TGG	-	chr2.2:12005912-12005931	None:intergenic	50.0%
	GCTCGTACGAATCCATGTGG+CGG	-	chr2.2:12005951-12005970	None:intergenic	55.0%
	GGGAAGATTGCCCTTGTCGT+CGG	-	chr2.2:12006037-12006056	None:intergenic	55.0%
	TTGGGTGCATGGATGACACC+AGG	-	chr2.2:12005901-12005920	None:intergenic	55.0%
!	GTTGTACGACAGTCTGGAGC+AGG	+	chr2.2:12006419-12006438	MS.gene84309:CDS	55.0%
	ACCTTACCACCTCCGCCACA+TGG	+	chr2.2:12005936-12005955	MS.gene84309:CDS	60.0%
	CGTACGAATCCATGTGGCGG+AGG	-	chr2.2:12005948-12005967	None:intergenic	60.0%
!	ACGAATCCATGTGGCGGAGG+TGG	-	chr2.2:12005945-12005964	None:intergenic	60.0%
!	GTACGACAGTCTGGAGCAGG+TGG	+	chr2.2:12006422-12006441	MS.gene84309:CDS	60.0%
!	TCCATGTGGCGGAGGTGGTA+AGG	-	chr2.2:12005940-12005959	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	12005757	12006617	12005757	ID=MS.gene84309
chr2.2	mRNA	12005757	12006617	12005757	ID=MS.gene84309.t1;Parent=MS.gene84309
chr2.2	exon	12005757	12006617	12005757	ID=MS.gene84309.t1.exon1;Parent=MS.gene84309.t1
chr2.2	CDS	12005757	12006617	12005757	ID=cds.MS.gene84309.t1;Parent=MS.gene84309.t1

Gene Sequence

>MS.gene84309

ATGAGCAGCAAGCCAAATCAGCCGTCTTCAACTAAACCTATTGTGATCCGTCAAGTTTGGTCTGACAATTTAGATTTAGAATTTGCTTATATCCGTAGCATCTTTCGCAAGTTTCGTTACATATCAATGTTTACAAAATTTCCTGGTGTCATCCATGCACCCAAAGTCGATGTCACTATACCTTACCACCTCCGCCACATGGATTCGTACGAGCAATACTCATATTTGAAGGCTAACGTCTATGATTTGAAACTTATCCAAGTCGGCCTTACATTAACCGACGACAAGGGCAATCTTCCCGATTTTGGAACAGATAACTCCTGCATATGGGAGTTCAATTTCAATGATTTTGATTTTGAACATGATCTTCAAAATCCAGACTCAGTTAATCTACTCTGCCATCAAGGGATCGACTTTGAACGCAATCGTTTATGTGGTGCCAGCTCTTCTCAGTTTGCTGAATTAATGTTCTCTTCAGGAATCCTTTTCAACGACCAAGTTGTTTGGGTGACTTTTCATAGCTCGTATGATTTCGGATATTTGGTGAAAGCTTTTACTGGGAGGAAGTTACCATCTCGCTTAGAGGAGTTTCTACGTCTCTTGACACAATTGTTTGGAAAAAATGTATACGACATCAAGTATATGATCAAGCATTGTAATGAGTTGTACGACAGTCTGGAGCAGGTGGCTAAATCACTACAAGTGAGTCGTGCAGTCGGGAATTCTCATCAAGCCGGCTCGGATAGTTTGTTGACCTGGTTAGCTTTCGATAAGATGGTCGACACTCATTTCATCAACAACAAAATCAACATCAACAAATATGCTGGAGTGCTCTTTGGATTGGAATTAACAGCAGCTTAA

Protein sequence

>MS.gene84309.t1

MSSKPNQPSSTKPIVIRQVWSDNLDLEFAYIRSIFRKFRYISMFTKFPGVIHAPKVDVTIPYHLRHMDSYEQYSYLKANVYDLKLIQVGLTLTDDKGNLPDFGTDNSCIWEFNFNDFDFEHDLQNPDSVNLLCHQGIDFERNRLCGASSSQFAELMFSSGILFNDQVVWVTFHSSYDFGYLVKAFTGRKLPSRLEEFLRLLTQLFGKNVYDIKYMIKHCNELYDSLEQVAKSLQVSRAVGNSHQAGSDSLLTWLAFDKMVDTHFINNKININKYAGVLFGLELTAA