Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84737.t1 | GAU47189.1 | 99.1 | 106 | 1 | 0 | 1 | 106 | 1 | 106 | 1.50E-56 | 228.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84737.t1 | Q9T096 | 66.7 | 102 | 34 | 0 | 1 | 102 | 1 | 102 | 1.7e-35 | 149.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84737.t1 | A0A2Z6PL80 | 99.1 | 106 | 1 | 0 | 1 | 106 | 1 | 106 | 1.1e-56 | 228.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene84737 | 0.813985 | 1.94E-51 | -1.69E-46 |
| MS.gene049711 | MS.gene84737 | 0.833842 | 4.23E-56 | -1.69E-46 |
| MS.gene050559 | MS.gene84737 | 0.866233 | 3.31E-65 | -1.69E-46 |
| MS.gene050561 | MS.gene84737 | 0.849555 | 3.00E-60 | -1.69E-46 |
| MS.gene050562 | MS.gene84737 | 0.853589 | 2.16E-61 | -1.69E-46 |
| MS.gene051201 | MS.gene84737 | 0.81432 | 1.64E-51 | -1.69E-46 |
| MS.gene051279 | MS.gene84737 | 0.827631 | 1.41E-54 | -1.69E-46 |
| MS.gene051744 | MS.gene84737 | 0.825768 | 3.91E-54 | -1.69E-46 |
| MS.gene052533 | MS.gene84737 | 0.838077 | 3.56E-57 | -1.69E-46 |
| MS.gene05539 | MS.gene84737 | 0.800949 | 1.13E-48 | -1.69E-46 |
| MS.gene05686 | MS.gene84737 | 0.850863 | 1.29E-60 | -1.69E-46 |
| MS.gene057102 | MS.gene84737 | 0.827287 | 1.70E-54 | -1.69E-46 |
| MS.gene059634 | MS.gene84737 | 0.802387 | 5.75E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene84737 | -0.837171 | 6.08E-57 | -1.69E-46 |
| MS.gene060525 | MS.gene84737 | 0.880742 | 4.27E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84737.t1 | MTR_8g027255 | 99.057 | 106 | 1 | 0 | 1 | 106 | 1 | 106 | 9.92e-77 | 221 |
| MS.gene84737.t1 | MTR_3g091840 | 68.571 | 105 | 33 | 0 | 1 | 105 | 1 | 105 | 5.67e-51 | 156 |
| MS.gene84737.t1 | MTR_1g077750 | 64.835 | 91 | 32 | 0 | 1 | 91 | 1 | 91 | 2.82e-43 | 137 |
| MS.gene84737.t1 | MTR_5g082490 | 49.495 | 99 | 50 | 0 | 8 | 106 | 12 | 110 | 6.70e-33 | 111 |
| MS.gene84737.t1 | MTR_3g070330 | 51.064 | 94 | 46 | 0 | 8 | 101 | 35 | 128 | 1.16e-32 | 111 |
| MS.gene84737.t1 | MTR_3g070330 | 51.648 | 91 | 44 | 0 | 8 | 98 | 35 | 125 | 1.95e-31 | 107 |
| MS.gene84737.t1 | MTR_4g088505 | 49.000 | 100 | 50 | 1 | 3 | 102 | 8 | 106 | 3.30e-30 | 104 |
| MS.gene84737.t1 | MTR_7g068250 | 47.423 | 97 | 51 | 0 | 10 | 106 | 14 | 110 | 2.28e-29 | 102 |
| MS.gene84737.t1 | MTR_2g078670 | 47.368 | 95 | 50 | 0 | 8 | 102 | 12 | 106 | 3.34e-28 | 99.8 |
| MS.gene84737.t1 | MTR_8g058785 | 43.158 | 95 | 54 | 0 | 8 | 102 | 12 | 106 | 8.29e-26 | 93.6 |
| MS.gene84737.t1 | MTR_8g061270 | 43.158 | 95 | 54 | 0 | 8 | 102 | 12 | 106 | 8.29e-26 | 93.6 |
| MS.gene84737.t1 | MTR_1g077760 | 69.091 | 55 | 17 | 0 | 44 | 98 | 1 | 55 | 6.26e-21 | 81.3 |
| MS.gene84737.t1 | MTR_4g088505 | 46.835 | 79 | 41 | 1 | 3 | 81 | 8 | 85 | 1.13e-20 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84737.t1 | AT4G27745 | 66.667 | 102 | 34 | 0 | 1 | 102 | 1 | 102 | 1.53e-47 | 147 |
| MS.gene84737.t1 | AT4G27745 | 66.667 | 102 | 34 | 0 | 1 | 102 | 1 | 102 | 1.53e-47 | 147 |
| MS.gene84737.t1 | AT4G27745 | 66.667 | 102 | 34 | 0 | 1 | 102 | 1 | 102 | 1.53e-47 | 147 |
| MS.gene84737.t1 | AT4G27745 | 66.667 | 102 | 34 | 0 | 1 | 102 | 1 | 102 | 1.53e-47 | 147 |
| MS.gene84737.t1 | AT3G11230 | 51.515 | 99 | 48 | 0 | 8 | 106 | 12 | 110 | 4.92e-35 | 117 |
| MS.gene84737.t1 | AT3G11230 | 53.191 | 94 | 44 | 0 | 8 | 101 | 45 | 138 | 1.56e-34 | 116 |
| MS.gene84737.t1 | AT3G11230 | 53.261 | 92 | 43 | 0 | 8 | 99 | 12 | 103 | 6.83e-34 | 113 |
| MS.gene84737.t1 | AT3G11230 | 53.846 | 91 | 42 | 0 | 8 | 98 | 45 | 135 | 1.78e-33 | 113 |
| MS.gene84737.t1 | AT4G27740 | 51.064 | 94 | 46 | 0 | 9 | 102 | 9 | 102 | 3.39e-33 | 111 |
| MS.gene84737.t1 | AT3G55890 | 51.485 | 101 | 48 | 1 | 1 | 101 | 6 | 105 | 1.12e-32 | 110 |
| MS.gene84737.t1 | AT3G55890 | 51.485 | 101 | 48 | 1 | 1 | 101 | 6 | 105 | 1.12e-32 | 110 |
| MS.gene84737.t1 | AT5G53940 | 49.474 | 95 | 48 | 0 | 8 | 102 | 12 | 106 | 7.68e-32 | 108 |
| MS.gene84737.t1 | AT3G55890 | 51.546 | 97 | 46 | 1 | 1 | 97 | 6 | 101 | 1.49e-31 | 107 |
| MS.gene84737.t1 | AT3G55890 | 51.546 | 97 | 46 | 1 | 1 | 97 | 6 | 101 | 1.49e-31 | 107 |
| MS.gene84737.t1 | AT2G40110 | 48.485 | 99 | 51 | 0 | 8 | 106 | 12 | 110 | 1.70e-31 | 108 |
| MS.gene84737.t1 | AT3G08990 | 49.020 | 102 | 51 | 1 | 1 | 102 | 6 | 106 | 3.29e-31 | 107 |
| MS.gene84737.t1 | AT3G08990 | 49.020 | 102 | 51 | 1 | 1 | 102 | 6 | 106 | 3.29e-31 | 107 |
| MS.gene84737.t1 | AT3G08990 | 49.020 | 102 | 51 | 1 | 1 | 102 | 6 | 106 | 3.29e-31 | 107 |
| MS.gene84737.t1 | AT2G40110 | 48.913 | 92 | 47 | 0 | 8 | 99 | 12 | 103 | 3.56e-30 | 103 |
| MS.gene84737.t1 | AT2G40110 | 43.836 | 73 | 41 | 0 | 8 | 80 | 12 | 84 | 5.21e-21 | 80.5 |
| MS.gene84737.t1 | AT2G40110 | 43.836 | 73 | 41 | 0 | 8 | 80 | 12 | 84 | 5.21e-21 | 80.5 |
Find 20 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGTTTCCTCATAAGCTTTA+AGG | 0.102549 | 8.4:-69682738 | None:intergenic |
| TGAGAAGTTTAAGATTGTTT+TGG | 0.258269 | 8.4:+69682789 | MS.gene84737:CDS |
| AAAGTTCACCTGAAAATCTT+TGG | 0.258418 | 8.4:-69681834 | None:intergenic |
| ATAAGCTGTCTATCTTCTTT+AGG | 0.260634 | 8.4:-69682649 | None:intergenic |
| ATCATTAATGGCCGATTTGA+TGG | 0.329137 | 8.4:+69681748 | None:intergenic |
| CATGGAAAGCAACAAGGTTC+TGG | 0.376925 | 8.4:-69681799 | None:intergenic |
| TGAGGAAACACAGAAGTATA+AGG | 0.390368 | 8.4:+69682750 | MS.gene84737:CDS |
| GAAATACCTTAAAGCTTATG+AGG | 0.433394 | 8.4:+69682732 | MS.gene84737:CDS |
| GGCCGATTTGATGGGATCAC+GGG | 0.488237 | 8.4:+69681757 | MS.gene84737:CDS |
| TGCCCGTGATCCCATCAAAT+CGG | 0.500160 | 8.4:-69681759 | None:intergenic |
| GATGTTTATTGTTCTGATTG+TGG | 0.528378 | 8.4:+69682694 | MS.gene84737:CDS |
| TCATTAATGGCCGATTTGAT+GGG | 0.534012 | 8.4:+69681749 | None:intergenic |
| TGGCCGATTTGATGGGATCA+CGG | 0.537450 | 8.4:+69681756 | MS.gene84737:CDS |
| TATGTTTAACAGGCAAGCAA+TGG | 0.540111 | 8.4:+69682583 | MS.gene84737:intron |
| CTTTGGAAACAATATCATCA+TGG | 0.545782 | 8.4:-69681817 | None:intergenic |
| TCTGATTGTGGAGAAGAACT+TGG | 0.556503 | 8.4:+69682706 | MS.gene84737:CDS |
| ATTGTGGAGAAGAACTTGGT+TGG | 0.558548 | 8.4:+69682710 | MS.gene84737:CDS |
| GAAGATAGACAGCTTATGAC+TGG | 0.563935 | 8.4:+69682655 | MS.gene84737:CDS |
| GAAACACAGAAGTATAAGGA+AGG | 0.612715 | 8.4:+69682754 | MS.gene84737:CDS |
| TATCATCATGGAAAGCAACA+AGG | 0.636612 | 8.4:-69681805 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAATTTGTTTAAATCAAA+TGG | + | chr8.4:69681989-69682008 | MS.gene84737:intron | 10.0% |
| !!! | AATTTACAATATGATTTTTT+TGG | - | chr8.4:69682400-69682419 | None:intergenic | 10.0% |
| !!! | TTAATTTGTTGAATATTAAT+TGG | + | chr8.4:69682497-69682516 | MS.gene84737:intron | 10.0% |
| !!! | AATTTTTTCTCTAAATCTAA+CGG | - | chr8.4:69681976-69681995 | None:intergenic | 15.0% |
| !!! | ACTTTTCTTTCTTTCAATTT+TGG | + | chr8.4:69681852-69681871 | MS.gene84737:intron | 20.0% |
| ! | AGAAGAAATGAAACAATCAT+TGG | + | chr8.4:69682299-69682318 | MS.gene84737:intron | 25.0% |
| ! | AGAGAAGTGAAATTAATTTC+AGG | - | chr8.4:69682210-69682229 | None:intergenic | 25.0% |
| ! | ATTAATTTCACTTCTCTCAA+TGG | + | chr8.4:69682213-69682232 | MS.gene84737:intron | 25.0% |
| ! | TGAATATTAATTGGACAAGA+GGG | + | chr8.4:69682506-69682525 | MS.gene84737:intron | 25.0% |
| ! | TTCATTTCTTCTGATCAATT+CGG | - | chr8.4:69682291-69682310 | None:intergenic | 25.0% |
| ! | TTGAATATTAATTGGACAAG+AGG | + | chr8.4:69682505-69682524 | MS.gene84737:intron | 25.0% |
| ! | TTTCAAAGGTTATGTTTAAC+AGG | + | chr8.4:69682573-69682592 | MS.gene84737:intron | 25.0% |
| !! | TAACCTCAAAAGATTTTCAA+AGG | + | chr8.4:69682559-69682578 | MS.gene84737:intron | 25.0% |
| !! | TATTAGATGATCCATTTTCA+TGG | - | chr8.4:69681925-69681944 | None:intergenic | 25.0% |
| !! | TATTGTTTCCAAAGATTTTC+AGG | + | chr8.4:69681826-69681845 | MS.gene84737:CDS | 25.0% |
| !!! | GAGAAGTTTAAGATTGTTTT+GGG | + | chr8.4:69682790-69682809 | MS.gene84737:CDS | 25.0% |
| !!! | TAACCTTTGAAAATCTTTTG+AGG | - | chr8.4:69682565-69682584 | None:intergenic | 25.0% |
| !!! | TGAGAAGTTTAAGATTGTTT+TGG | + | chr8.4:69682789-69682808 | MS.gene84737:CDS | 25.0% |
| !!! | TTAAGATTGTTTTGGGAAAT+GGG | + | chr8.4:69682797-69682816 | MS.gene84737:CDS | 25.0% |
| !!! | TTTAAGATTGTTTTGGGAAA+TGG | + | chr8.4:69682796-69682815 | MS.gene84737:CDS | 25.0% |
| AAACGATTCATCCATGAAAA+TGG | + | chr8.4:69681911-69681930 | MS.gene84737:intron | 30.0% | |
| AAAGTTCACCTGAAAATCTT+TGG | - | chr8.4:69681837-69681856 | None:intergenic | 30.0% | |
| AAATGGATCATCTAATACTG+TGG | + | chr8.4:69681928-69681947 | MS.gene84737:intron | 30.0% | |
| AGCTTTCATCGTGATTATAT+TGG | - | chr8.4:69682174-69682193 | None:intergenic | 30.0% | |
| ATAAGCTGTCTATCTTCTTT+AGG | - | chr8.4:69682652-69682671 | None:intergenic | 30.0% | |
| GAAATACCTTAAAGCTTATG+AGG | + | chr8.4:69682732-69682751 | MS.gene84737:CDS | 30.0% | |
| GATGTTTATTGTTCTGATTG+TGG | + | chr8.4:69682694-69682713 | MS.gene84737:CDS | 30.0% | |
| GCTTTCATCGTGATTATATT+GGG | - | chr8.4:69682173-69682192 | None:intergenic | 30.0% | |
| TAAAGGAAATGGACAAAATC+TGG | - | chr8.4:69682354-69682373 | None:intergenic | 30.0% | |
| ! | AGATTTTGTCCATTTCCTTT+AGG | + | chr8.4:69682353-69682372 | MS.gene84737:intron | 30.0% |
| ! | CATGCCATGAACATATTTTT+GGG | + | chr8.4:69682625-69682644 | MS.gene84737:CDS | 30.0% |
| ! | CTTTGGAAACAATATCATCA+TGG | - | chr8.4:69681820-69681839 | None:intergenic | 30.0% |
| ! | TCATGCCATGAACATATTTT+TGG | + | chr8.4:69682624-69682643 | MS.gene84737:CDS | 30.0% |
| !!! | TTGGTTTTAATTTAACCCTC+TGG | - | chr8.4:69682381-69682400 | None:intergenic | 30.0% |
| ACCCAAAAAATGTCTTCTAG+TGG | - | chr8.4:69682072-69682091 | None:intergenic | 35.0% | |
| AGGTCCCAAAAATATGTTCA+TGG | - | chr8.4:69682632-69682651 | None:intergenic | 35.0% | |
| ATATTAATTGGACAAGAGGG+TGG | + | chr8.4:69682509-69682528 | MS.gene84737:intron | 35.0% | |
| CCCAAAAAATGTCTTCTAGT+GGG | - | chr8.4:69682071-69682090 | None:intergenic | 35.0% | |
| GTGTTTCCTCATAAGCTTTA+AGG | - | chr8.4:69682741-69682760 | None:intergenic | 35.0% | |
| TATCATCATGGAAAGCAACA+AGG | - | chr8.4:69681808-69681827 | None:intergenic | 35.0% | |
| TATGTTTAACAGGCAAGCAA+TGG | + | chr8.4:69682583-69682602 | MS.gene84737:intron | 35.0% | |
| TGCTTAACGAGCTTCAATTA+AGG | + | chr8.4:69682265-69682284 | MS.gene84737:intron | 35.0% | |
| ! | AAAATGTCTTCTAGTGGGTA+TGG | - | chr8.4:69682066-69682085 | None:intergenic | 35.0% |
| ! | GAAACACAGAAGTATAAGGA+AGG | + | chr8.4:69682754-69682773 | MS.gene84737:CDS | 35.0% |
| ! | TGAGGAAACACAGAAGTATA+AGG | + | chr8.4:69682750-69682769 | MS.gene84737:CDS | 35.0% |
| !!! | ACCCACTAGAAGACATTTTT+TGG | + | chr8.4:69682067-69682086 | MS.gene84737:intron | 35.0% |
| !!! | CCCACTAGAAGACATTTTTT+GGG | + | chr8.4:69682068-69682087 | MS.gene84737:intron | 35.0% |
| AATCAAATGGTTCCTACCAC+AGG | + | chr8.4:69682002-69682021 | MS.gene84737:intron | 40.0% | |
| ATCAAATGGTTCCTACCACA+GGG | + | chr8.4:69682003-69682022 | MS.gene84737:intron | 40.0% | |
| ATTGTGGAGAAGAACTTGGT+TGG | + | chr8.4:69682710-69682729 | MS.gene84737:CDS | 40.0% | |
| CAGGGATGTTCTTCTACTTA+TGG | + | chr8.4:69682021-69682040 | MS.gene84737:intron | 40.0% | |
| GAAGATAGACAGCTTATGAC+TGG | + | chr8.4:69682655-69682674 | MS.gene84737:CDS | 40.0% | |
| TAAGTAGAAGAACATCCCTG+TGG | - | chr8.4:69682021-69682040 | None:intergenic | 40.0% | |
| TCTGATTGTGGAGAAGAACT+TGG | + | chr8.4:69682706-69682725 | MS.gene84737:CDS | 40.0% | |
| TGGGTATGGATGAACGAAAT+TGG | - | chr8.4:69682052-69682071 | None:intergenic | 40.0% | |
| ! | CATTTCCTTTAGGAACCAGA+GGG | + | chr8.4:69682363-69682382 | MS.gene84737:intron | 40.0% |
| ! | TTTAACCCTCTGGTTCCTAA+AGG | - | chr8.4:69682371-69682390 | None:intergenic | 40.0% |
| CATGGAAAGCAACAAGGTTC+TGG | - | chr8.4:69681802-69681821 | None:intergenic | 45.0% | |
| TAATTGGACAAGAGGGTGGT+TGG | + | chr8.4:69682513-69682532 | MS.gene84737:intron | 45.0% | |
| TAGAAGAACATCCCTGTGGT+AGG | - | chr8.4:69682017-69682036 | None:intergenic | 45.0% | |
| ! | ACTGTGGAATAGTGTTGCTG+CGG | + | chr8.4:69681944-69681963 | MS.gene84737:intron | 45.0% |
| ! | CCATTTCCTTTAGGAACCAG+AGG | + | chr8.4:69682362-69682381 | MS.gene84737:intron | 45.0% |
| ! | CCTCTGGTTCCTAAAGGAAA+TGG | - | chr8.4:69682365-69682384 | None:intergenic | 45.0% |
| TGCCCGTGATCCCATCAAAT+CGG | - | chr8.4:69681762-69681781 | None:intergenic | 50.0% | |
| !! | TGGCCGATTTGATGGGATCA+CGG | + | chr8.4:69681756-69681775 | MS.gene84737:CDS | 50.0% |
| !! | GGCCGATTTGATGGGATCAC+GGG | + | chr8.4:69681757-69681776 | MS.gene84737:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 69681755 | 69682822 | 69681755 | ID=MS.gene84737 |
| chr8.4 | mRNA | 69681755 | 69682822 | 69681755 | ID=MS.gene84737.t1;Parent=MS.gene84737 |
| chr8.4 | exon | 69681755 | 69681847 | 69681755 | ID=MS.gene84737.t1.exon1;Parent=MS.gene84737.t1 |
| chr8.4 | CDS | 69681755 | 69681847 | 69681755 | ID=cds.MS.gene84737.t1;Parent=MS.gene84737.t1 |
| chr8.4 | exon | 69682595 | 69682822 | 69682595 | ID=MS.gene84737.t1.exon2;Parent=MS.gene84737.t1 |
| chr8.4 | CDS | 69682595 | 69682822 | 69682595 | ID=cds.MS.gene84737.t1;Parent=MS.gene84737.t1 |
| Gene Sequence |
| Protein sequence |
