Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85904.t1 | XP_024639118.1 | 91.5 | 282 | 24 | 0 | 1 | 282 | 1 | 282 | 1.20E-148 | 535.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85904.t1 | Q9FMS6 | 55.8 | 274 | 115 | 3 | 10 | 280 | 7 | 277 | 3.7e-82 | 306.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85904.t1 | A0A396HQ65 | 91.5 | 282 | 24 | 0 | 1 | 282 | 1 | 282 | 8.7e-149 | 535.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene053225 | MS.gene85904 | PPI |
| MS.gene85904 | MS.gene21541 | PPI |
| MS.gene034816 | MS.gene85904 | PPI |
| MS.gene72977 | MS.gene85904 | PPI |
| MS.gene72978 | MS.gene85904 | PPI |
| MS.gene034815 | MS.gene85904 | PPI |
| MS.gene025629 | MS.gene85904 | PPI |
| MS.gene034250 | MS.gene85904 | PPI |
| MS.gene41100 | MS.gene85904 | PPI |
| MS.gene065709 | MS.gene85904 | PPI |
| MS.gene85904 | MS.gene025628 | PPI |
| MS.gene007154 | MS.gene85904 | PPI |
| MS.gene000609 | MS.gene85904 | PPI |
| MS.gene85904 | MS.gene41097 | PPI |
| MS.gene065707 | MS.gene85904 | PPI |
| MS.gene41101 | MS.gene85904 | PPI |
| MS.gene065708 | MS.gene85904 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85904.t1 | AT5G22250 | 55.839 | 274 | 115 | 3 | 10 | 280 | 7 | 277 | 1.29e-104 | 306 |
| MS.gene85904.t1 | AT3G44260 | 55.019 | 269 | 112 | 5 | 15 | 280 | 17 | 279 | 2.14e-98 | 290 |
| MS.gene85904.t1 | AT5G10960 | 46.909 | 275 | 137 | 6 | 7 | 280 | 1 | 267 | 5.41e-82 | 248 |
| MS.gene85904.t1 | AT2G32070 | 48.315 | 267 | 129 | 6 | 15 | 280 | 10 | 268 | 3.14e-80 | 244 |
| MS.gene85904.t1 | AT1G80780 | 46.241 | 266 | 135 | 5 | 15 | 280 | 10 | 267 | 3.07e-76 | 233 |
| MS.gene85904.t1 | AT1G80780 | 46.241 | 266 | 135 | 5 | 15 | 280 | 10 | 267 | 3.07e-76 | 233 |
| MS.gene85904.t1 | AT1G80780 | 46.241 | 266 | 135 | 5 | 15 | 280 | 10 | 267 | 4.18e-76 | 233 |
| MS.gene85904.t1 | AT1G15920 | 45.324 | 278 | 142 | 6 | 9 | 282 | 8 | 279 | 2.22e-72 | 224 |
| MS.gene85904.t1 | AT1G15920 | 45.324 | 278 | 142 | 6 | 9 | 282 | 8 | 279 | 2.22e-72 | 224 |
| MS.gene85904.t1 | AT1G61470 | 38.866 | 247 | 124 | 9 | 19 | 259 | 4 | 229 | 1.41e-40 | 142 |
| MS.gene85904.t1 | AT1G06450 | 34.173 | 278 | 154 | 11 | 10 | 281 | 3 | 257 | 4.32e-37 | 135 |
| MS.gene85904.t1 | AT1G27820 | 36.471 | 255 | 133 | 10 | 13 | 259 | 2 | 235 | 2.14e-34 | 127 |
| MS.gene85904.t1 | AT1G27890 | 37.500 | 248 | 128 | 10 | 19 | 259 | 3 | 230 | 4.92e-33 | 123 |
| MS.gene85904.t1 | AT3G44240 | 36.052 | 233 | 128 | 9 | 31 | 260 | 3 | 217 | 1.94e-31 | 117 |
Find 56 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATGGCGCTTATGATTT+TGG | 0.117389 | 5.3:-30791810 | MS.gene85904:CDS |
| AAATACGCTGGAGTAGTATT+TGG | 0.224745 | 5.3:-30791513 | MS.gene85904:CDS |
| TGAATACGGTGAATATTCTT+TGG | 0.284182 | 5.3:-30792210 | MS.gene85904:CDS |
| CAAACTTCTCTGACAATGAT+TGG | 0.307379 | 5.3:+30792281 | None:intergenic |
| CTTGGACTCACTTTAATAGA+TGG | 0.314324 | 5.3:-30792062 | MS.gene85904:CDS |
| CAATCATTGTCAGAGAAGTT+TGG | 0.315172 | 5.3:-30792280 | MS.gene85904:CDS |
| AAGAAATCTGCCAGATAGTT+TGG | 0.326893 | 5.3:-30791763 | MS.gene85904:CDS |
| AATCATTGTCAGAGAAGTTT+GGG | 0.392755 | 5.3:-30792279 | MS.gene85904:CDS |
| AAAGAATATTCACCGTATTC+AGG | 0.394726 | 5.3:+30792212 | None:intergenic |
| CGATTATCGGTGTCAAAATC+AGG | 0.397384 | 5.3:+30792026 | None:intergenic |
| AGTTTAAGATCATCAACATT+TGG | 0.398324 | 5.3:+30792092 | None:intergenic |
| ACACCGATAATCGCTACATT+TGG | 0.399135 | 5.3:-30792016 | MS.gene85904:CDS |
| AACTATCTGGCAGATTTCTT+CGG | 0.406991 | 5.3:+30791766 | None:intergenic |
| TTGCCAAATGTAGCGATTAT+CGG | 0.410093 | 5.3:+30792013 | None:intergenic |
| CTCAGTGTGGATCGGGTTGT+TGG | 0.420495 | 5.3:-30791627 | MS.gene85904:CDS |
| CGTGATGTTCTTCCTGAATA+CGG | 0.423867 | 5.3:-30792224 | MS.gene85904:CDS |
| TCGATAGACACTGAATTTCC+TGG | 0.427087 | 5.3:-30792185 | MS.gene85904:CDS |
| AGGTGGCGATGGTTGACTTT+TGG | 0.431272 | 5.3:+30792149 | None:intergenic |
| CATTGTATGGTGGTCTTGAT+CGG | 0.438293 | 5.3:-30791662 | MS.gene85904:CDS |
| CGCTACAGCAATGCATTGTA+TGG | 0.451428 | 5.3:-30791675 | MS.gene85904:CDS |
| TGTCAACAAGCTGTCGGATC+CGG | 0.457649 | 5.3:+30791590 | None:intergenic |
| TATTCAAGAAATCAATGACT+TGG | 0.469568 | 5.3:-30791839 | MS.gene85904:CDS |
| TGATTTCTTGAATAGAAGTC+CGG | 0.475089 | 5.3:+30791848 | None:intergenic |
| ATGGCAGACGTTTCATAAGT+TGG | 0.479969 | 5.3:-30791568 | MS.gene85904:CDS |
| TGTAGTAATCAGAGGGTTGA+AGG | 0.480186 | 5.3:+30792129 | None:intergenic |
| AAGTACACTCAGTGTGGATC+GGG | 0.482322 | 5.3:-30791634 | MS.gene85904:CDS |
| ATTGTATGGTGGTCTTGATC+GGG | 0.514058 | 5.3:-30791661 | MS.gene85904:CDS |
| CAATGGACTCTTTGTTGTGA+GGG | 0.524178 | 5.3:+30791958 | None:intergenic |
| CAAGTACACTCAGTGTGGAT+CGG | 0.525306 | 5.3:-30791635 | MS.gene85904:CDS |
| TTGTCGACGAAGCAAATCAA+TGG | 0.528289 | 5.3:+30791941 | None:intergenic |
| GCTGAACTAATGTTGACGTC+CGG | 0.531198 | 5.3:-30791867 | MS.gene85904:CDS |
| GTGTGGATCGGGTTGTTGGA+AGG | 0.531402 | 5.3:-30791623 | MS.gene85904:CDS |
| TTCAATCGTTGTGTCTCTCA+AGG | 0.540052 | 5.3:-30791909 | MS.gene85904:CDS |
| GTTGGAAGGTCTCATCAATC+CGG | 0.540408 | 5.3:-30791609 | MS.gene85904:CDS |
| ATTCAAGAAATCAATGACTT+GGG | 0.542864 | 5.3:-30791838 | MS.gene85904:CDS |
| GATCTTAAACTCATCCAACT+TGG | 0.545194 | 5.3:-30792080 | MS.gene85904:CDS |
| CAGAGGGTTGAAGGTGGCGA+TGG | 0.554259 | 5.3:+30792138 | None:intergenic |
| CTATTAAAGTGAGTCCAAGT+TGG | 0.558411 | 5.3:+30792066 | None:intergenic |
| TTTGGTGTATGGATAACTCC+AGG | 0.559457 | 5.3:+30792167 | None:intergenic |
| CATACAATGCATTGCTGTAG+CGG | 0.560379 | 5.3:+30791676 | None:intergenic |
| CACGCTCGACGTTGAAGTCA+CGG | 0.560577 | 5.3:+30791982 | None:intergenic |
| TTCGGATCAATATCTTCACC+AGG | 0.564436 | 5.3:+30791784 | None:intergenic |
| GAAGAACATCACGAATGAGA+TGG | 0.565749 | 5.3:+30792234 | None:intergenic |
| ATTGATTTGCTTCGTCGACA+AGG | 0.569126 | 5.3:-30791939 | MS.gene85904:CDS |
| TCAACATTAGTTCAGCAAAA+CGG | 0.571611 | 5.3:+30791874 | None:intergenic |
| GATCCGACAGCTTGTTGACA+TGG | 0.577163 | 5.3:-30791587 | MS.gene85904:CDS |
| AACGTATCTGTAGTAATCAG+AGG | 0.584888 | 5.3:+30792121 | None:intergenic |
| TTGATTTGCTTCGTCGACAA+GGG | 0.585778 | 5.3:-30791938 | MS.gene85904:CDS |
| TCAATGGACTCTTTGTTGTG+AGG | 0.592655 | 5.3:+30791957 | None:intergenic |
| CTCACTTTAATAGATGGTTG+CGG | 0.607202 | 5.3:-30792056 | MS.gene85904:CDS |
| CTGCCATGTCAACAAGCTGT+CGG | 0.613501 | 5.3:+30791584 | None:intergenic |
| ACGTATCTGTAGTAATCAGA+GGG | 0.616993 | 5.3:+30792122 | None:intergenic |
| TACAGCAATGCATTGTATGG+TGG | 0.620242 | 5.3:-30791672 | MS.gene85904:CDS |
| TATCGGTGTCAAAATCAGGA+AGG | 0.683593 | 5.3:+30792030 | None:intergenic |
| AGTAATCAGAGGGTTGAAGG+TGG | 0.690515 | 5.3:+30792132 | None:intergenic |
| GGTTGCAAGTACACTCAGTG+TGG | 0.697230 | 5.3:-30791640 | MS.gene85904:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACATTTTGGAAATGTTATT+CGG | - | chr5.3:30792085-30792104 | MS.gene85904:CDS | 20.0% |
| ! | AATGAATTTGAAAAATACGC+TGG | - | chr5.3:30792289-30792308 | MS.gene85904:CDS | 25.0% |
| ! | ATTCAAGAAATCAATGACTT+GGG | - | chr5.3:30791976-30791995 | MS.gene85904:CDS | 25.0% |
| ! | TATTCAAGAAATCAATGACT+TGG | - | chr5.3:30791975-30791994 | MS.gene85904:CDS | 25.0% |
| !! | AGTTTAAGATCATCAACATT+TGG | + | chr5.3:30791725-30791744 | None:intergenic | 25.0% |
| AAAAACTCTTCCAAACTATC+TGG | + | chr5.3:30792064-30792083 | None:intergenic | 30.0% | |
| AAAGAATATTCACCGTATTC+AGG | + | chr5.3:30791605-30791624 | None:intergenic | 30.0% | |
| AATCATTGTCAGAGAAGTTT+GGG | - | chr5.3:30791535-30791554 | MS.gene85904:CDS | 30.0% | |
| TCAACATTAGTTCAGCAAAA+CGG | + | chr5.3:30791943-30791962 | None:intergenic | 30.0% | |
| TGAATACGGTGAATATTCTT+TGG | - | chr5.3:30791604-30791623 | MS.gene85904:CDS | 30.0% | |
| TGATTTCTTGAATAGAAGTC+CGG | + | chr5.3:30791969-30791988 | None:intergenic | 30.0% | |
| ! | GGAAGAGTTTTTGAACATTT+TGG | - | chr5.3:30792072-30792091 | MS.gene85904:CDS | 30.0% |
| !! | TTTTGGAAATGTTATTCGGA+AGG | - | chr5.3:30792089-30792108 | MS.gene85904:CDS | 30.0% |
| AAATACGCTGGAGTAGTATT+TGG | - | chr5.3:30792301-30792320 | MS.gene85904:CDS | 35.0% | |
| AACGTATCTGTAGTAATCAG+AGG | + | chr5.3:30791696-30791715 | None:intergenic | 35.0% | |
| AACTATCTGGCAGATTTCTT+CGG | + | chr5.3:30792051-30792070 | None:intergenic | 35.0% | |
| AAGAAATCTGCCAGATAGTT+TGG | - | chr5.3:30792051-30792070 | MS.gene85904:CDS | 35.0% | |
| ACGTATCTGTAGTAATCAGA+GGG | + | chr5.3:30791695-30791714 | None:intergenic | 35.0% | |
| CAAACTTCTCTGACAATGAT+TGG | + | chr5.3:30791536-30791555 | None:intergenic | 35.0% | |
| CAATCATTGTCAGAGAAGTT+TGG | - | chr5.3:30791534-30791553 | MS.gene85904:CDS | 35.0% | |
| CTATTAAAGTGAGTCCAAGT+TGG | + | chr5.3:30791751-30791770 | None:intergenic | 35.0% | |
| CTCACTTTAATAGATGGTTG+CGG | - | chr5.3:30791758-30791777 | MS.gene85904:CDS | 35.0% | |
| CTTGGACTCACTTTAATAGA+TGG | - | chr5.3:30791752-30791771 | MS.gene85904:CDS | 35.0% | |
| GATCTTAAACTCATCCAACT+TGG | - | chr5.3:30791734-30791753 | MS.gene85904:CDS | 35.0% | |
| TTGCCAAATGTAGCGATTAT+CGG | + | chr5.3:30791804-30791823 | None:intergenic | 35.0% | |
| ! | TTTCATGGCGCTTATGATTT+TGG | - | chr5.3:30792004-30792023 | MS.gene85904:CDS | 35.0% |
| ACACCGATAATCGCTACATT+TGG | - | chr5.3:30791798-30791817 | MS.gene85904:CDS | 40.0% | |
| ATGGCAGACGTTTCATAAGT+TGG | - | chr5.3:30792246-30792265 | MS.gene85904:CDS | 40.0% | |
| CATACAATGCATTGCTGTAG+CGG | + | chr5.3:30792141-30792160 | None:intergenic | 40.0% | |
| CGTGATGTTCTTCCTGAATA+CGG | - | chr5.3:30791590-30791609 | MS.gene85904:CDS | 40.0% | |
| GAAGAACATCACGAATGAGA+TGG | + | chr5.3:30791583-30791602 | None:intergenic | 40.0% | |
| TACAGCAATGCATTGTATGG+TGG | - | chr5.3:30792142-30792161 | MS.gene85904:CDS | 40.0% | |
| TCGATAGACACTGAATTTCC+TGG | - | chr5.3:30791629-30791648 | MS.gene85904:CDS | 40.0% | |
| TGTAGTAATCAGAGGGTTGA+AGG | + | chr5.3:30791688-30791707 | None:intergenic | 40.0% | |
| TTCAATCGTTGTGTCTCTCA+AGG | - | chr5.3:30791905-30791924 | MS.gene85904:CDS | 40.0% | |
| TTCGGATCAATATCTTCACC+AGG | + | chr5.3:30792033-30792052 | None:intergenic | 40.0% | |
| TTGTCGACGAAGCAAATCAA+TGG | + | chr5.3:30791876-30791895 | None:intergenic | 40.0% | |
| ! | ATTGTATGGTGGTCTTGATC+GGG | - | chr5.3:30792153-30792172 | MS.gene85904:CDS | 40.0% |
| ! | CAATGGACTCTTTGTTGTGA+GGG | + | chr5.3:30791859-30791878 | None:intergenic | 40.0% |
| ! | CATTGTATGGTGGTCTTGAT+CGG | - | chr5.3:30792152-30792171 | MS.gene85904:CDS | 40.0% |
| ! | CGCTTATGATTTTGGATACC+TGG | - | chr5.3:30792012-30792031 | MS.gene85904:CDS | 40.0% |
| ! | TCAATGGACTCTTTGTTGTG+AGG | + | chr5.3:30791860-30791879 | None:intergenic | 40.0% |
| ! | TTTGGTGTATGGATAACTCC+AGG | + | chr5.3:30791650-30791669 | None:intergenic | 40.0% |
| !! | ATGACTTGGGTGACTTTTCA+TGG | - | chr5.3:30791989-30792008 | MS.gene85904:CDS | 40.0% |
| !! | ATTGATTTGCTTCGTCGACA+AGG | - | chr5.3:30791875-30791894 | MS.gene85904:CDS | 40.0% |
| !! | CGATTATCGGTGTCAAAATC+AGG | + | chr5.3:30791791-30791810 | None:intergenic | 40.0% |
| !! | GATGGTTGACTTTTGGTGTA+TGG | + | chr5.3:30791661-30791680 | None:intergenic | 40.0% |
| !! | TATCGGTGTCAAAATCAGGA+AGG | + | chr5.3:30791787-30791806 | None:intergenic | 40.0% |
| !! | TTGATTTGCTTCGTCGACAA+GGG | - | chr5.3:30791876-30791895 | MS.gene85904:CDS | 40.0% |
| AAGTACACTCAGTGTGGATC+GGG | - | chr5.3:30792180-30792199 | MS.gene85904:CDS | 45.0% | |
| AGTAATCAGAGGGTTGAAGG+TGG | + | chr5.3:30791685-30791704 | None:intergenic | 45.0% | |
| CAAGTACACTCAGTGTGGAT+CGG | - | chr5.3:30792179-30792198 | MS.gene85904:CDS | 45.0% | |
| CGCTACAGCAATGCATTGTA+TGG | - | chr5.3:30792139-30792158 | MS.gene85904:CDS | 45.0% | |
| GCTGAACTAATGTTGACGTC+CGG | - | chr5.3:30791947-30791966 | MS.gene85904:CDS | 45.0% | |
| GTTGGAAGGTCTCATCAATC+CGG | - | chr5.3:30792205-30792224 | MS.gene85904:CDS | 45.0% | |
| CTGCCATGTCAACAAGCTGT+CGG | + | chr5.3:30792233-30792252 | None:intergenic | 50.0% | |
| GGTTGCAAGTACACTCAGTG+TGG | - | chr5.3:30792174-30792193 | MS.gene85904:CDS | 50.0% | |
| TGTCAACAAGCTGTCGGATC+CGG | + | chr5.3:30792227-30792246 | None:intergenic | 50.0% | |
| !! | AGGTGGCGATGGTTGACTTT+TGG | + | chr5.3:30791668-30791687 | None:intergenic | 50.0% |
| !! | GATCCGACAGCTTGTTGACA+TGG | - | chr5.3:30792227-30792246 | MS.gene85904:CDS | 50.0% |
| CACGCTCGACGTTGAAGTCA+CGG | + | chr5.3:30791835-30791854 | None:intergenic | 55.0% | |
| ! | CTCAGTGTGGATCGGGTTGT+TGG | - | chr5.3:30792187-30792206 | MS.gene85904:CDS | 55.0% |
| ! | GTGTGGATCGGGTTGTTGGA+AGG | - | chr5.3:30792191-30792210 | MS.gene85904:CDS | 55.0% |
| CAGAGGGTTGAAGGTGGCGA+TGG | + | chr5.3:30791679-30791698 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 30791494 | 30792342 | 30791494 | ID=MS.gene85904 |
| chr5.3 | mRNA | 30791494 | 30792342 | 30791494 | ID=MS.gene85904.t1;Parent=MS.gene85904 |
| chr5.3 | exon | 30791494 | 30792342 | 30791494 | ID=MS.gene85904.t1.exon1;Parent=MS.gene85904.t1 |
| chr5.3 | CDS | 30791494 | 30792342 | 30791494 | ID=cds.MS.gene85904.t1;Parent=MS.gene85904.t1 |
| Gene Sequence |
| Protein sequence |
