Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85905.t1 | XP_024639118.1 | 91.1 | 282 | 25 | 0 | 1 | 282 | 1 | 282 | 1.10E-149 | 539.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85905.t1 | Q9FMS6 | 57.3 | 274 | 111 | 3 | 10 | 280 | 7 | 277 | 1.1e-84 | 314.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85905.t1 | A0A396HQ65 | 91.1 | 282 | 25 | 0 | 1 | 282 | 1 | 282 | 7.9e-150 | 539.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene034815 | MS.gene85905 | PPI |
| MS.gene025629 | MS.gene85905 | PPI |
| MS.gene034250 | MS.gene85905 | PPI |
| MS.gene034816 | MS.gene85905 | PPI |
| MS.gene72977 | MS.gene85905 | PPI |
| MS.gene85905 | MS.gene065707 | PPI |
| MS.gene065709 | MS.gene85905 | PPI |
| MS.gene72978 | MS.gene85905 | PPI |
| MS.gene85905 | MS.gene21541 | PPI |
| MS.gene85905 | MS.gene41097 | PPI |
| MS.gene41100 | MS.gene85905 | PPI |
| MS.gene000609 | MS.gene85905 | PPI |
| MS.gene85905 | MS.gene41101 | PPI |
| MS.gene065708 | MS.gene85905 | PPI |
| MS.gene053225 | MS.gene85905 | PPI |
| MS.gene85905 | MS.gene007154 | PPI |
| MS.gene85905 | MS.gene025628 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85905.t1 | AT5G22250 | 57.299 | 274 | 111 | 3 | 10 | 280 | 7 | 277 | 8.47e-108 | 314 |
| MS.gene85905.t1 | AT3G44260 | 55.762 | 269 | 110 | 5 | 15 | 280 | 17 | 279 | 5.59e-100 | 294 |
| MS.gene85905.t1 | AT2G32070 | 48.507 | 268 | 129 | 6 | 14 | 280 | 9 | 268 | 2.07e-81 | 247 |
| MS.gene85905.t1 | AT5G10960 | 46.545 | 275 | 138 | 6 | 7 | 280 | 1 | 267 | 2.23e-81 | 247 |
| MS.gene85905.t1 | AT1G80780 | 46.617 | 266 | 134 | 5 | 15 | 280 | 10 | 267 | 8.69e-77 | 235 |
| MS.gene85905.t1 | AT1G80780 | 46.617 | 266 | 134 | 5 | 15 | 280 | 10 | 267 | 8.69e-77 | 235 |
| MS.gene85905.t1 | AT1G80780 | 46.617 | 266 | 134 | 5 | 15 | 280 | 10 | 267 | 1.35e-76 | 235 |
| MS.gene85905.t1 | AT1G15920 | 45.324 | 278 | 142 | 6 | 9 | 282 | 8 | 279 | 1.17e-72 | 225 |
| MS.gene85905.t1 | AT1G15920 | 45.324 | 278 | 142 | 6 | 9 | 282 | 8 | 279 | 1.17e-72 | 225 |
| MS.gene85905.t1 | AT1G61470 | 38.618 | 246 | 126 | 8 | 19 | 259 | 4 | 229 | 3.17e-42 | 146 |
| MS.gene85905.t1 | AT1G06450 | 34.572 | 269 | 148 | 10 | 16 | 278 | 8 | 254 | 4.04e-38 | 137 |
| MS.gene85905.t1 | AT1G27820 | 36.220 | 254 | 135 | 9 | 13 | 259 | 2 | 235 | 3.40e-36 | 131 |
| MS.gene85905.t1 | AT1G27890 | 37.247 | 247 | 130 | 9 | 19 | 259 | 3 | 230 | 1.10e-34 | 127 |
| MS.gene85905.t1 | AT3G44240 | 35.193 | 233 | 130 | 8 | 31 | 260 | 3 | 217 | 1.49e-32 | 120 |
Find 58 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATGGCGCTTATGATTT+TGG | 0.117389 | 5.3:-30796947 | MS.gene85905:CDS |
| AAATACGCTGGAGTAGTATT+TGG | 0.224745 | 5.3:-30796650 | MS.gene85905:CDS |
| AAGAAATTTGCCTGATAGTT+TGG | 0.274713 | 5.3:-30796900 | MS.gene85905:CDS |
| TGAATATGGTGAATATTCTT+TGG | 0.278766 | 5.3:-30797347 | MS.gene85905:CDS |
| CGTGATGTTCTTCCTGAATA+TGG | 0.278966 | 5.3:-30797361 | MS.gene85905:CDS |
| CAATCATTGTCCGAGAAGTT+TGG | 0.285300 | 5.3:-30797417 | MS.gene85905:CDS |
| CTTGGACTCACTTTAATAGA+TGG | 0.314324 | 5.3:-30797199 | MS.gene85905:CDS |
| CAAACTTCTCGGACAATGAT+TGG | 0.324520 | 5.3:+30797418 | None:intergenic |
| GCTGAACTAATGTTGACTTC+TGG | 0.360897 | 5.3:-30797004 | MS.gene85905:CDS |
| TCGATAGACACCGAGTTTCC+TGG | 0.362514 | 5.3:-30797322 | MS.gene85905:CDS |
| AATCATTGTCCGAGAAGTTT+GGG | 0.374317 | 5.3:-30797416 | MS.gene85905:CDS |
| AGATGGTTGCGGTCACCTTC+CGG | 0.382019 | 5.3:-30797182 | MS.gene85905:CDS |
| AGTTTAAGATCATCAACATT+TGG | 0.398324 | 5.3:+30797229 | None:intergenic |
| ACACCGATAATCGCTACATT+TGG | 0.399135 | 5.3:-30797153 | MS.gene85905:CDS |
| CTCAATGTGGATCGGGTTGT+TGG | 0.408633 | 5.3:-30796764 | MS.gene85905:CDS |
| TTGCCAAATGTAGCGATTAT+CGG | 0.410093 | 5.3:+30797150 | None:intergenic |
| AACTATCAGGCAAATTTCTT+CGG | 0.413248 | 5.3:+30796903 | None:intergenic |
| AAAGAATATTCACCATATTC+AGG | 0.416250 | 5.3:+30797349 | None:intergenic |
| AGGTGGCGATGGTCGAGTTT+TGG | 0.428542 | 5.3:+30797286 | None:intergenic |
| CATTGTATGGTGGTCTTGAT+CGG | 0.438293 | 5.3:-30796799 | MS.gene85905:CDS |
| CGATTATCGGTGTCAAAATC+CGG | 0.449608 | 5.3:+30797163 | None:intergenic |
| TGTCAACAAGCTGTCGGATC+CGG | 0.457649 | 5.3:+30796727 | None:intergenic |
| AGTTGTGTGCCCAAACTTCT+CGG | 0.466420 | 5.3:+30797407 | None:intergenic |
| TATTCAAGAAATCAATGACT+TGG | 0.469568 | 5.3:-30796976 | MS.gene85905:CDS |
| CGCTACTGCAATGCATTGTA+TGG | 0.477422 | 5.3:-30796812 | MS.gene85905:CDS |
| ATGGCAGACGTTTCATAAGT+TGG | 0.479969 | 5.3:-30796705 | MS.gene85905:CDS |
| TGTAGTAATCAGAGGGTTGA+AGG | 0.480186 | 5.3:+30797266 | None:intergenic |
| GTTGACTTGCTTCGTCGACA+AGG | 0.507118 | 5.3:-30797076 | MS.gene85905:CDS |
| ATTGTATGGTGGTCTTGATC+GGG | 0.514058 | 5.3:-30796798 | MS.gene85905:CDS |
| AAGTACACTCAATGTGGATC+GGG | 0.524067 | 5.3:-30796771 | MS.gene85905:CDS |
| TTGACTTGCTTCGTCGACAA+GGG | 0.527074 | 5.3:-30797075 | MS.gene85905:CDS |
| TTTGGTGTATGGATAACTCC+AGG | 0.533788 | 5.3:+30797304 | None:intergenic |
| TTCAATCGTTGTGTCTCTCA+AGG | 0.540052 | 5.3:-30797046 | MS.gene85905:CDS |
| GTTGGAAGGTCTCATCAATC+CGG | 0.540408 | 5.3:-30796746 | MS.gene85905:CDS |
| ATGTGGATCGGGTTGTTGGA+AGG | 0.540595 | 5.3:-30796760 | MS.gene85905:CDS |
| ATTCAAGAAATCAATGACTT+GGG | 0.542864 | 5.3:-30796975 | MS.gene85905:CDS |
| GATCTTAAACTCATCCAACT+TGG | 0.545194 | 5.3:-30797217 | MS.gene85905:CDS |
| CAAGTACACTCAATGTGGAT+CGG | 0.548190 | 5.3:-30796772 | MS.gene85905:CDS |
| CACGCTCAACGTTGAAGTCA+CGG | 0.550608 | 5.3:+30797119 | None:intergenic |
| CTATTAAAGTGAGTCCAAGT+TGG | 0.558411 | 5.3:+30797203 | None:intergenic |
| CAGAGGGTTGAAGGTGGCGA+TGG | 0.559485 | 5.3:+30797275 | None:intergenic |
| TCAACATTAGTTCAGCAAAA+CGG | 0.564358 | 5.3:+30797011 | None:intergenic |
| GAAGAACATCACGAATGAGA+TGG | 0.565749 | 5.3:+30797371 | None:intergenic |
| GATCCGACAGCTTGTTGACA+TGG | 0.577163 | 5.3:-30796724 | MS.gene85905:CDS |
| CATACAATGCATTGCAGTAG+CGG | 0.583860 | 5.3:+30796813 | None:intergenic |
| GACGTATCTGTAGTAATCAG+AGG | 0.593946 | 5.3:+30797258 | None:intergenic |
| TACTGCAATGCATTGTATGG+TGG | 0.597106 | 5.3:-30796809 | MS.gene85905:CDS |
| CAACGGAGTCCTTGTTGTGA+GGG | 0.597538 | 5.3:+30797095 | None:intergenic |
| ATGGATAACTCCAGGAAACT+CGG | 0.598400 | 5.3:+30797312 | None:intergenic |
| ACGTATCTGTAGTAATCAGA+GGG | 0.599199 | 5.3:+30797259 | None:intergenic |
| TCAACGGAGTCCTTGTTGTG+AGG | 0.600831 | 5.3:+30797094 | None:intergenic |
| CTCACTTTAATAGATGGTTG+CGG | 0.607202 | 5.3:-30797193 | MS.gene85905:CDS |
| CTGCCATGTCAACAAGCTGT+CGG | 0.613501 | 5.3:+30796721 | None:intergenic |
| TTGTCGACGAAGCAAGTCAA+CGG | 0.630986 | 5.3:+30797078 | None:intergenic |
| GGTTGCAAGTACACTCAATG+TGG | 0.665808 | 5.3:-30796777 | MS.gene85905:CDS |
| TGAGCGTGACCCTCACAACA+AGG | 0.670125 | 5.3:-30797104 | MS.gene85905:CDS |
| TATCGGTGTCAAAATCCGGA+AGG | 0.680978 | 5.3:+30797167 | None:intergenic |
| AGTAATCAGAGGGTTGAAGG+TGG | 0.690515 | 5.3:+30797269 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACATTTTGGAAATGTTATT+CGG | - | chr5.3:30797222-30797241 | MS.gene85905:CDS | 20.0% |
| ! | AAAGAATATTCACCATATTC+AGG | + | chr5.3:30796742-30796761 | None:intergenic | 25.0% |
| ! | AAAGAATTTGAAAAATACGC+TGG | - | chr5.3:30797426-30797445 | MS.gene85905:CDS | 25.0% |
| ! | ATTCAAGAAATCAATGACTT+GGG | - | chr5.3:30797113-30797132 | MS.gene85905:CDS | 25.0% |
| ! | TATTCAAGAAATCAATGACT+TGG | - | chr5.3:30797112-30797131 | MS.gene85905:CDS | 25.0% |
| ! | TGAATATGGTGAATATTCTT+TGG | - | chr5.3:30796741-30796760 | MS.gene85905:CDS | 25.0% |
| !! | AGTTTAAGATCATCAACATT+TGG | + | chr5.3:30796862-30796881 | None:intergenic | 25.0% |
| AAAAACTCTTCCAAACTATC+AGG | + | chr5.3:30797201-30797220 | None:intergenic | 30.0% | |
| AACTATCAGGCAAATTTCTT+CGG | + | chr5.3:30797188-30797207 | None:intergenic | 30.0% | |
| AAGAAATTTGCCTGATAGTT+TGG | - | chr5.3:30797188-30797207 | MS.gene85905:CDS | 30.0% | |
| TCAACATTAGTTCAGCAAAA+CGG | + | chr5.3:30797080-30797099 | None:intergenic | 30.0% | |
| ! | GGAAGAGTTTTTGAACATTT+TGG | - | chr5.3:30797209-30797228 | MS.gene85905:CDS | 30.0% |
| !! | TTTTGGAAATGTTATTCGGA+AGG | - | chr5.3:30797226-30797245 | MS.gene85905:CDS | 30.0% |
| AAATACGCTGGAGTAGTATT+TGG | - | chr5.3:30797438-30797457 | MS.gene85905:CDS | 35.0% | |
| AATCATTGTCCGAGAAGTTT+GGG | - | chr5.3:30796672-30796691 | MS.gene85905:CDS | 35.0% | |
| ACGTATCTGTAGTAATCAGA+GGG | + | chr5.3:30796832-30796851 | None:intergenic | 35.0% | |
| CTATTAAAGTGAGTCCAAGT+TGG | + | chr5.3:30796888-30796907 | None:intergenic | 35.0% | |
| CTCACTTTAATAGATGGTTG+CGG | - | chr5.3:30796895-30796914 | MS.gene85905:CDS | 35.0% | |
| CTTGGACTCACTTTAATAGA+TGG | - | chr5.3:30796889-30796908 | MS.gene85905:CDS | 35.0% | |
| GATCTTAAACTCATCCAACT+TGG | - | chr5.3:30796871-30796890 | MS.gene85905:CDS | 35.0% | |
| TTGCCAAATGTAGCGATTAT+CGG | + | chr5.3:30796941-30796960 | None:intergenic | 35.0% | |
| ! | CGCTTATGATTTTGGATACT+TGG | - | chr5.3:30797149-30797168 | MS.gene85905:CDS | 35.0% |
| ! | TTTCATGGCGCTTATGATTT+TGG | - | chr5.3:30797141-30797160 | MS.gene85905:CDS | 35.0% |
| AAGTACACTCAATGTGGATC+GGG | - | chr5.3:30797317-30797336 | MS.gene85905:CDS | 40.0% | |
| ACACCGATAATCGCTACATT+TGG | - | chr5.3:30796935-30796954 | MS.gene85905:CDS | 40.0% | |
| ATGGATAACTCCAGGAAACT+CGG | + | chr5.3:30796779-30796798 | None:intergenic | 40.0% | |
| ATGGCAGACGTTTCATAAGT+TGG | - | chr5.3:30797383-30797402 | MS.gene85905:CDS | 40.0% | |
| CAAACTTCTCGGACAATGAT+TGG | + | chr5.3:30796673-30796692 | None:intergenic | 40.0% | |
| CAAGTACACTCAATGTGGAT+CGG | - | chr5.3:30797316-30797335 | MS.gene85905:CDS | 40.0% | |
| CAATCATTGTCCGAGAAGTT+TGG | - | chr5.3:30796671-30796690 | MS.gene85905:CDS | 40.0% | |
| CATACAATGCATTGCAGTAG+CGG | + | chr5.3:30797278-30797297 | None:intergenic | 40.0% | |
| CGTGATGTTCTTCCTGAATA+TGG | - | chr5.3:30796727-30796746 | MS.gene85905:CDS | 40.0% | |
| GAAGAACATCACGAATGAGA+TGG | + | chr5.3:30796720-30796739 | None:intergenic | 40.0% | |
| GACGTATCTGTAGTAATCAG+AGG | + | chr5.3:30796833-30796852 | None:intergenic | 40.0% | |
| TACTGCAATGCATTGTATGG+TGG | - | chr5.3:30797279-30797298 | MS.gene85905:CDS | 40.0% | |
| TGTAGTAATCAGAGGGTTGA+AGG | + | chr5.3:30796825-30796844 | None:intergenic | 40.0% | |
| TTCAATCGTTGTGTCTCTCA+AGG | - | chr5.3:30797042-30797061 | MS.gene85905:CDS | 40.0% | |
| ! | ATTGTATGGTGGTCTTGATC+GGG | - | chr5.3:30797290-30797309 | MS.gene85905:CDS | 40.0% |
| ! | CATTGTATGGTGGTCTTGAT+CGG | - | chr5.3:30797289-30797308 | MS.gene85905:CDS | 40.0% |
| ! | TTTGGTGTATGGATAACTCC+AGG | + | chr5.3:30796787-30796806 | None:intergenic | 40.0% |
| !! | ATGACTTGGGTGACTTTTCA+TGG | - | chr5.3:30797126-30797145 | MS.gene85905:CDS | 40.0% |
| !! | CGATTATCGGTGTCAAAATC+CGG | + | chr5.3:30796928-30796947 | None:intergenic | 40.0% |
| !! | GCTGAACTAATGTTGACTTC+TGG | - | chr5.3:30797084-30797103 | MS.gene85905:CDS | 40.0% |
| AGTAATCAGAGGGTTGAAGG+TGG | + | chr5.3:30796822-30796841 | None:intergenic | 45.0% | |
| AGTTGTGTGCCCAAACTTCT+CGG | + | chr5.3:30796684-30796703 | None:intergenic | 45.0% | |
| CGCTACTGCAATGCATTGTA+TGG | - | chr5.3:30797276-30797295 | MS.gene85905:CDS | 45.0% | |
| GGTTGCAAGTACACTCAATG+TGG | - | chr5.3:30797311-30797330 | MS.gene85905:CDS | 45.0% | |
| GTTGGAAGGTCTCATCAATC+CGG | - | chr5.3:30797342-30797361 | MS.gene85905:CDS | 45.0% | |
| TTGTCGACGAAGCAAGTCAA+CGG | + | chr5.3:30797013-30797032 | None:intergenic | 45.0% | |
| !! | GATGGTCGAGTTTTGGTGTA+TGG | + | chr5.3:30796798-30796817 | None:intergenic | 45.0% |
| !! | TATCGGTGTCAAAATCCGGA+AGG | + | chr5.3:30796924-30796943 | None:intergenic | 45.0% |
| !! | TTGACTTGCTTCGTCGACAA+GGG | - | chr5.3:30797013-30797032 | MS.gene85905:CDS | 45.0% |
| CACGCTCAACGTTGAAGTCA+CGG | + | chr5.3:30796972-30796991 | None:intergenic | 50.0% | |
| CTGCCATGTCAACAAGCTGT+CGG | + | chr5.3:30797370-30797389 | None:intergenic | 50.0% | |
| TCGATAGACACCGAGTTTCC+TGG | - | chr5.3:30796766-30796785 | MS.gene85905:CDS | 50.0% | |
| TGTCAACAAGCTGTCGGATC+CGG | + | chr5.3:30797364-30797383 | None:intergenic | 50.0% | |
| ! | ATGTGGATCGGGTTGTTGGA+AGG | - | chr5.3:30797328-30797347 | MS.gene85905:CDS | 50.0% |
| ! | CAACGGAGTCCTTGTTGTGA+GGG | + | chr5.3:30796996-30797015 | None:intergenic | 50.0% |
| ! | CTCAATGTGGATCGGGTTGT+TGG | - | chr5.3:30797324-30797343 | MS.gene85905:CDS | 50.0% |
| ! | TCAACGGAGTCCTTGTTGTG+AGG | + | chr5.3:30796997-30797016 | None:intergenic | 50.0% |
| !! | GATCCGACAGCTTGTTGACA+TGG | - | chr5.3:30797364-30797383 | MS.gene85905:CDS | 50.0% |
| !! | GTTGACTTGCTTCGTCGACA+AGG | - | chr5.3:30797012-30797031 | MS.gene85905:CDS | 50.0% |
| AGATGGTTGCGGTCACCTTC+CGG | - | chr5.3:30796906-30796925 | MS.gene85905:CDS | 55.0% | |
| TGAGCGTGACCCTCACAACA+AGG | - | chr5.3:30796984-30797003 | MS.gene85905:CDS | 55.0% | |
| ! | AGGTGGCGATGGTCGAGTTT+TGG | + | chr5.3:30796805-30796824 | None:intergenic | 55.0% |
| CAGAGGGTTGAAGGTGGCGA+TGG | + | chr5.3:30796816-30796835 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 30796631 | 30797479 | 30796631 | ID=MS.gene85905 |
| chr5.3 | mRNA | 30796631 | 30797479 | 30796631 | ID=MS.gene85905.t1;Parent=MS.gene85905 |
| chr5.3 | exon | 30796631 | 30797479 | 30796631 | ID=MS.gene85905.t1.exon1;Parent=MS.gene85905.t1 |
| chr5.3 | CDS | 30796631 | 30797479 | 30796631 | ID=cds.MS.gene85905.t1;Parent=MS.gene85905.t1 |
| Gene Sequence |
| Protein sequence |
