Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86311.t1 | KEH39790.1 | 98.4 | 125 | 2 | 0 | 1 | 125 | 1 | 125 | 1.50E-63 | 251.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86311.t1 | A2X0N9 | 44.6 | 83 | 44 | 1 | 42 | 124 | 62 | 142 | 9.6e-14 | 77.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86311.t1 | A0A072VNS6 | 98.4 | 125 | 2 | 0 | 1 | 125 | 1 | 125 | 1.1e-63 | 251.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene86311 | 0.817534 | 3.14E-52 | -1.69E-46 |
| MS.gene049680 | MS.gene86311 | 0.80361 | 3.21E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene86311 | 0.818628 | 1.77E-52 | -1.69E-46 |
| MS.gene053094 | MS.gene86311 | 0.80129 | 9.66E-49 | -1.69E-46 |
| MS.gene053963 | MS.gene86311 | 0.874916 | 4.64E-68 | -1.69E-46 |
| MS.gene054222 | MS.gene86311 | 0.860149 | 2.53E-63 | -1.69E-46 |
| MS.gene054957 | MS.gene86311 | 0.803876 | 2.83E-49 | -1.69E-46 |
| MS.gene055150 | MS.gene86311 | 0.823551 | 1.30E-53 | -1.69E-46 |
| MS.gene055226 | MS.gene86311 | 0.803328 | 3.67E-49 | -1.69E-46 |
| MS.gene05624 | MS.gene86311 | 0.815195 | 1.05E-51 | -1.69E-46 |
| MS.gene058592 | MS.gene86311 | 0.832835 | 7.53E-56 | -1.69E-46 |
| MS.gene05878 | MS.gene86311 | 0.806297 | 8.81E-50 | -1.69E-46 |
| MS.gene059051 | MS.gene86311 | 0.804926 | 1.71E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene86311 | 0.854341 | 1.31E-61 | -1.69E-46 |
| MS.gene05985 | MS.gene86311 | -0.808587 | 2.88E-50 | -1.69E-46 |
| MS.gene060012 | MS.gene86311 | 0.836867 | 7.28E-57 | -1.69E-46 |
| MS.gene06018 | MS.gene86311 | 0.843236 | 1.59E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86311.t1 | MTR_1g010050 | 98.400 | 125 | 2 | 0 | 1 | 125 | 1 | 125 | 3.51e-89 | 254 |
| MS.gene86311.t1 | MTR_1g010050 | 98.969 | 97 | 1 | 0 | 1 | 97 | 1 | 97 | 1.40e-66 | 196 |
| MS.gene86311.t1 | MTR_1g010050 | 97.561 | 82 | 2 | 0 | 1 | 82 | 1 | 82 | 7.40e-54 | 164 |
| MS.gene86311.t1 | MTR_1g071100 | 34.951 | 103 | 62 | 2 | 23 | 125 | 11 | 108 | 3.05e-17 | 72.0 |
| MS.gene86311.t1 | MTR_1g076640 | 38.393 | 112 | 61 | 2 | 14 | 125 | 4 | 107 | 1.84e-13 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86311.t1 | AT3G63420 | 36.170 | 94 | 56 | 2 | 32 | 125 | 8 | 97 | 4.17e-16 | 68.6 |
| MS.gene86311.t1 | AT3G63420 | 36.170 | 94 | 56 | 2 | 32 | 125 | 8 | 97 | 4.17e-16 | 68.6 |
| MS.gene86311.t1 | AT3G63420 | 39.759 | 83 | 46 | 2 | 32 | 114 | 48 | 126 | 4.44e-16 | 69.7 |
| MS.gene86311.t1 | AT3G22942 | 44.000 | 75 | 40 | 1 | 45 | 119 | 21 | 93 | 1.89e-11 | 57.0 |
Find 27 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTCTTTCCCTGGCTTCTA+TGG | 0.223765 | 1.3:+10163856 | MS.gene86311:CDS |
| CTGGTTCTGGTTGGGATAGA+TGG | 0.293101 | 1.3:+10164746 | MS.gene86311:CDS |
| TAACATGCTTGCAGGATTAA+AGG | 0.337522 | 1.3:+10164712 | MS.gene86311:intron |
| TCTGGTTGGGATAGATGGTT+TGG | 0.341402 | 1.3:+10164751 | MS.gene86311:CDS |
| GGTCCAGTTGATGCTGGTTC+TGG | 0.360151 | 1.3:+10164733 | MS.gene86311:CDS |
| TGACCTGGAGAATATTGATT+TGG | 0.373161 | 1.3:-10163918 | None:intergenic |
| GTTGGAACTGTTTCTTTCCC+TGG | 0.413466 | 1.3:+10163847 | MS.gene86311:CDS |
| GAATATTGATTTGGTTATTG+AGG | 0.423057 | 1.3:-10163909 | None:intergenic |
| TTGGTTATTGAGGTTAGATA+TGG | 0.427698 | 1.3:-10163899 | None:intergenic |
| CAACCAGAACCAGCATCAAC+TGG | 0.434691 | 1.3:-10164736 | None:intergenic |
| CCTACTTCATTTGCACAAGT+TGG | 0.435219 | 1.3:+10163829 | MS.gene86311:CDS |
| GAAGTAACAGATTCAGAAGA+TGG | 0.451442 | 1.3:+10163766 | MS.gene86311:CDS |
| TACCATGGAAGCATAGGATC+TGG | 0.459697 | 1.3:-10164392 | None:intergenic |
| GGTTGGGATAGATGGTTTGG+AGG | 0.467119 | 1.3:+10164754 | MS.gene86311:CDS |
| AGTTGATGCTGGTTCTGGTT+GGG | 0.472706 | 1.3:+10164738 | MS.gene86311:CDS |
| TATGATAATGCAGGAAGAGT+TGG | 0.480511 | 1.3:+10164201 | MS.gene86311:intron |
| ATTCATTGAAAAGAAAGAAA+AGG | 0.487861 | 1.3:+10163804 | MS.gene86311:CDS |
| TATTGATTGCTGTCTATCCA+TGG | 0.496767 | 1.3:-10163743 | None:intergenic |
| CAGTTGATGCTGGTTCTGGT+TGG | 0.505214 | 1.3:+10164737 | MS.gene86311:CDS |
| ATTAAAGGTCCAGTTGATGC+TGG | 0.509550 | 1.3:+10164727 | MS.gene86311:CDS |
| CCAACTTGTGCAAATGAAGT+AGG | 0.510578 | 1.3:-10163829 | None:intergenic |
| TAACCAAATCAATATTCTCC+AGG | 0.514948 | 1.3:+10163915 | MS.gene86311:CDS |
| TGCCAGATCCTATGCTTCCA+TGG | 0.550978 | 1.3:+10164390 | MS.gene86311:CDS |
| ACTCTCGAAATCATAAGCGC+TGG | 0.564148 | 1.3:+10164785 | MS.gene86311:CDS |
| TGGAAGCATAGGATCTGGCA+TGG | 0.601609 | 1.3:-10164387 | None:intergenic |
| TGCTTACTCATTGCAGACCG+TGG | 0.621212 | 1.3:-10164251 | None:intergenic |
| AACAATAGGTGAATCTTCCA+CGG | 0.624066 | 1.3:+10164234 | MS.gene86311:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTTACATGTTATATATA+TGG | + | chr1.3:10164138-10164157 | MS.gene86311:intron | 10.0% |
| !!! | AAAACAAATACTTTTTAAAT+TGG | + | chr1.3:10164285-10164304 | MS.gene86311:intron | 10.0% |
| !!! | AAATTAGATATAGTTTTTTT+TGG | - | chr1.3:10164081-10164100 | None:intergenic | 10.0% |
| !!! | AATTAGATATAGTTTTTTTT+GGG | - | chr1.3:10164080-10164099 | None:intergenic | 10.0% |
| !!! | AAATACTTTTTAAATTGGAT+TGG | + | chr1.3:10164290-10164309 | MS.gene86311:intron | 15.0% |
| !!! | ATTGTTGTGTATTTTTTAAA+CGG | + | chr1.3:10164470-10164489 | MS.gene86311:intron | 15.0% |
| !! | ATTCATTGAAAAGAAAGAAA+AGG | + | chr1.3:10163804-10163823 | MS.gene86311:CDS | 20.0% |
| !! | CAAAAACTTATATATCTTCT+TGG | + | chr1.3:10164000-10164019 | MS.gene86311:intron | 20.0% |
| !!! | ACTCACAATATTACATTTTA+AGG | + | chr1.3:10164441-10164460 | MS.gene86311:intron | 20.0% |
| !!! | TTAATTAAAAGCACAATGAA+TGG | - | chr1.3:10164340-10164359 | None:intergenic | 20.0% |
| !!! | TTGTTGTGTATTTTTTAAAC+GGG | + | chr1.3:10164471-10164490 | MS.gene86311:intron | 20.0% |
| ! | TTACATGTTATCAACTCATA+TGG | - | chr1.3:10164695-10164714 | None:intergenic | 25.0% |
| ! | TTGGAAAAACTTGAAACAAT+AGG | + | chr1.3:10164220-10164239 | MS.gene86311:CDS | 25.0% |
| !! | AGATGATCTTAATTTTACCA+TGG | - | chr1.3:10164410-10164429 | None:intergenic | 25.0% |
| !! | ATTTGAACTTATGATAATGC+AGG | + | chr1.3:10164192-10164211 | MS.gene86311:intron | 25.0% |
| !!! | GAATATTGATTTGGTTATTG+AGG | - | chr1.3:10163912-10163931 | None:intergenic | 25.0% |
| !!! | TGCTATTTTTCATCATTGAA+TGG | + | chr1.3:10164313-10164332 | MS.gene86311:intron | 25.0% |
| TAACCAAATCAATATTCTCC+AGG | + | chr1.3:10163915-10163934 | MS.gene86311:CDS | 30.0% | |
| TGCAGAACTATATATCTTCT+TGG | + | chr1.3:10164109-10164128 | MS.gene86311:intron | 30.0% | |
| TGTTATCAACTCATATGGTT+AGG | - | chr1.3:10164690-10164709 | None:intergenic | 30.0% | |
| TTGGTTATTGAGGTTAGATA+TGG | - | chr1.3:10163902-10163921 | None:intergenic | 30.0% | |
| !! | TAATTTTACCATGGAAGCAT+AGG | - | chr1.3:10164401-10164420 | None:intergenic | 30.0% |
| !! | TTAAAAAGGGTCTTGTTAAC+CGG | - | chr1.3:10164626-10164645 | None:intergenic | 30.0% |
| AACAATAGGTGAATCTTCCA+CGG | + | chr1.3:10164234-10164253 | MS.gene86311:CDS | 35.0% | |
| ATAACATGTAACATGCTTGC+AGG | + | chr1.3:10164704-10164723 | MS.gene86311:intron | 35.0% | |
| GAAGTAACAGATTCAGAAGA+TGG | + | chr1.3:10163766-10163785 | MS.gene86311:CDS | 35.0% | |
| TAACATGCTTGCAGGATTAA+AGG | + | chr1.3:10164712-10164731 | MS.gene86311:intron | 35.0% | |
| ! | TATGATAATGCAGGAAGAGT+TGG | + | chr1.3:10164201-10164220 | MS.gene86311:intron | 35.0% |
| ! | TATGTTTTCCATAGAAGCCA+GGG | - | chr1.3:10163867-10163886 | None:intergenic | 35.0% |
| ! | TGACCTGGAGAATATTGATT+TGG | - | chr1.3:10163921-10163940 | None:intergenic | 35.0% |
| AAATGAGATGAGAGTTGACC+TGG | - | chr1.3:10163936-10163955 | None:intergenic | 40.0% | |
| CCAACTTGTGCAAATGAAGT+AGG | - | chr1.3:10163832-10163851 | None:intergenic | 40.0% | |
| CCTACTTCATTTGCACAAGT+TGG | + | chr1.3:10163829-10163848 | MS.gene86311:CDS | 40.0% | |
| ! | ATTAAAGGTCCAGTTGATGC+TGG | + | chr1.3:10164727-10164746 | MS.gene86311:CDS | 40.0% |
| ! | CTATGTTTTCCATAGAAGCC+AGG | - | chr1.3:10163868-10163887 | None:intergenic | 40.0% |
| AACTAGTGTCGCGTAAACAC+CGG | + | chr1.3:10164604-10164623 | MS.gene86311:intron | 45.0% | |
| ACTCTCGAAATCATAAGCGC+TGG | + | chr1.3:10164785-10164804 | MS.gene86311:CDS | 45.0% | |
| GTTTCTTTCCCTGGCTTCTA+TGG | + | chr1.3:10163856-10163875 | MS.gene86311:CDS | 45.0% | |
| TCTGGTTGGGATAGATGGTT+TGG | + | chr1.3:10164751-10164770 | MS.gene86311:CDS | 45.0% | |
| ! | GTTGGAACTGTTTCTTTCCC+TGG | + | chr1.3:10163847-10163866 | MS.gene86311:CDS | 45.0% |
| ! | TACCATGGAAGCATAGGATC+TGG | - | chr1.3:10164395-10164414 | None:intergenic | 45.0% |
| !! | AGTTGATGCTGGTTCTGGTT+GGG | + | chr1.3:10164738-10164757 | MS.gene86311:CDS | 45.0% |
| !!! | ATTATTTGTTATATTTAAAA+AGG | - | chr1.3:10164640-10164659 | None:intergenic | 5.0% |
| !!! | TTATTTATATTTTAGTATTA+TGG | + | chr1.3:10163959-10163978 | MS.gene86311:intron | 5.0% |
| !!! | TTATTTGTTATATTTAAAAA+GGG | - | chr1.3:10164639-10164658 | None:intergenic | 5.0% |
| CAACCAGAACCAGCATCAAC+TGG | - | chr1.3:10164739-10164758 | None:intergenic | 50.0% | |
| TGCCAGATCCTATGCTTCCA+TGG | + | chr1.3:10164390-10164409 | MS.gene86311:CDS | 50.0% | |
| TGCTTACTCATTGCAGACCG+TGG | - | chr1.3:10164254-10164273 | None:intergenic | 50.0% | |
| ! | GGTTGGGATAGATGGTTTGG+AGG | + | chr1.3:10164754-10164773 | MS.gene86311:CDS | 50.0% |
| ! | TGGAAGCATAGGATCTGGCA+TGG | - | chr1.3:10164390-10164409 | None:intergenic | 50.0% |
| !! | CAGTTGATGCTGGTTCTGGT+TGG | + | chr1.3:10164737-10164756 | MS.gene86311:CDS | 50.0% |
| !! | CTGGTTCTGGTTGGGATAGA+TGG | + | chr1.3:10164746-10164765 | MS.gene86311:CDS | 50.0% |
| !! | GGTCCAGTTGATGCTGGTTC+TGG | + | chr1.3:10164733-10164752 | MS.gene86311:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 10163745 | 10164813 | 10163745 | ID=MS.gene86311 |
| chr1.3 | mRNA | 10163745 | 10164813 | 10163745 | ID=MS.gene86311.t1;Parent=MS.gene86311 |
| chr1.3 | exon | 10163745 | 10163936 | 10163745 | ID=MS.gene86311.t1.exon1;Parent=MS.gene86311.t1 |
| chr1.3 | CDS | 10163745 | 10163936 | 10163745 | ID=cds.MS.gene86311.t1;Parent=MS.gene86311.t1 |
| chr1.3 | exon | 10164214 | 10164266 | 10164214 | ID=MS.gene86311.t1.exon2;Parent=MS.gene86311.t1 |
| chr1.3 | CDS | 10164214 | 10164266 | 10164214 | ID=cds.MS.gene86311.t1;Parent=MS.gene86311.t1 |
| chr1.3 | exon | 10164367 | 10164411 | 10164367 | ID=MS.gene86311.t1.exon3;Parent=MS.gene86311.t1 |
| chr1.3 | CDS | 10164367 | 10164411 | 10164367 | ID=cds.MS.gene86311.t1;Parent=MS.gene86311.t1 |
| chr1.3 | exon | 10164726 | 10164813 | 10164726 | ID=MS.gene86311.t1.exon4;Parent=MS.gene86311.t1 |
| chr1.3 | CDS | 10164726 | 10164813 | 10164726 | ID=cds.MS.gene86311.t1;Parent=MS.gene86311.t1 |
| Gene Sequence |
| Protein sequence |
