Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059051.t1 | XP_003594839.1 | 73.2 | 157 | 7 | 1 | 1 | 122 | 1 | 157 | 2.30E-51 | 211.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059051.t1 | Q06931 | 65.0 | 157 | 20 | 1 | 1 | 122 | 1 | 157 | 2.4e-46 | 186.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059051.t1 | G7INA7 | 73.2 | 157 | 7 | 1 | 1 | 122 | 1 | 157 | 1.6e-51 | 211.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053963 | MS.gene059051 | 0.820439 | 6.85E-53 | -1.69E-46 |
| MS.gene059051 | MS.gene066513 | 0.844645 | 6.65E-59 | -1.69E-46 |
| MS.gene059051 | MS.gene068565 | 0.80546 | 1.32E-49 | -1.69E-46 |
| MS.gene059051 | MS.gene071846 | 0.821793 | 3.34E-53 | -1.69E-46 |
| MS.gene059051 | MS.gene073239 | 0.808467 | 3.05E-50 | -1.69E-46 |
| MS.gene059051 | MS.gene073760 | 0.800381 | 1.48E-48 | -1.69E-46 |
| MS.gene059051 | MS.gene21776 | 0.813721 | 2.22E-51 | -1.69E-46 |
| MS.gene059051 | MS.gene30085 | 0.801523 | 8.65E-49 | -1.69E-46 |
| MS.gene059051 | MS.gene31855 | 0.808464 | 3.06E-50 | -1.69E-46 |
| MS.gene059051 | MS.gene32801 | 0.846527 | 2.05E-59 | -1.69E-46 |
| MS.gene059051 | MS.gene34444 | 0.82685 | 2.16E-54 | -1.69E-46 |
| MS.gene059051 | MS.gene51427 | 0.823172 | 1.60E-53 | -1.69E-46 |
| MS.gene059051 | MS.gene51428 | 0.805384 | 1.37E-49 | -1.69E-46 |
| MS.gene059051 | MS.gene53537 | 0.805048 | 1.61E-49 | -1.69E-46 |
| MS.gene059051 | MS.gene58653 | 0.801225 | 9.95E-49 | -1.69E-46 |
| MS.gene059051 | MS.gene62676 | 0.822103 | 2.83E-53 | -1.69E-46 |
| MS.gene059051 | MS.gene62677 | 0.836913 | 7.08E-57 | -1.69E-46 |
| MS.gene059051 | MS.gene70739 | 0.835272 | 1.85E-56 | -1.69E-46 |
| MS.gene059051 | MS.gene71735 | 0.800569 | 1.36E-48 | -1.69E-46 |
| MS.gene059051 | MS.gene72620 | 0.802385 | 5.75E-49 | -1.69E-46 |
| MS.gene059051 | MS.gene74530 | 0.806762 | 7.03E-50 | -1.69E-46 |
| MS.gene059051 | MS.gene82269 | 0.820457 | 6.79E-53 | -1.69E-46 |
| MS.gene059051 | MS.gene84484 | 0.850966 | 1.21E-60 | -1.69E-46 |
| MS.gene059051 | MS.gene86311 | 0.804926 | 1.71E-49 | -1.69E-46 |
| MS.gene059051 | MS.gene88351 | 0.831424 | 1.68E-55 | -1.69E-46 |
| MS.gene059051 | MS.gene95655 | 0.803855 | 2.86E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059051.t1 | MTR_2g035220 | 73.248 | 157 | 7 | 1 | 1 | 122 | 1 | 157 | 1.49e-72 | 213 |
| MS.gene059051.t1 | MTR_2g035210 | 70.701 | 157 | 11 | 1 | 1 | 122 | 1 | 157 | 6.19e-70 | 207 |
| MS.gene059051.t1 | MTR_2g035190 | 70.701 | 157 | 11 | 1 | 1 | 122 | 1 | 157 | 2.87e-69 | 205 |
| MS.gene059051.t1 | MTR_2g035320 | 66.879 | 157 | 17 | 1 | 1 | 122 | 1 | 157 | 2.57e-66 | 197 |
| MS.gene059051.t1 | MTR_2g035320 | 86.458 | 96 | 13 | 0 | 27 | 122 | 27 | 122 | 1.19e-56 | 172 |
| MS.gene059051.t1 | MTR_6g033450 | 51.592 | 157 | 41 | 1 | 1 | 122 | 1 | 157 | 3.58e-44 | 141 |
| MS.gene059051.t1 | MTR_4g120970 | 51.592 | 157 | 41 | 1 | 1 | 122 | 1 | 157 | 3.58e-44 | 141 |
| MS.gene059051.t1 | MTR_2g035150 | 48.408 | 157 | 46 | 1 | 1 | 122 | 1 | 157 | 9.60e-40 | 130 |
| MS.gene059051.t1 | MTR_2g035100 | 46.497 | 157 | 49 | 1 | 1 | 122 | 1 | 157 | 4.84e-39 | 128 |
| MS.gene059051.t1 | MTR_2g035105 | 46.497 | 157 | 49 | 1 | 1 | 122 | 1 | 157 | 4.95e-39 | 128 |
| MS.gene059051.t1 | MTR_2g035130 | 45.860 | 157 | 50 | 1 | 1 | 122 | 1 | 157 | 4.94e-38 | 126 |
| MS.gene059051.t1 | MTR_2g035120 | 44.586 | 157 | 52 | 2 | 1 | 122 | 1 | 157 | 1.83e-34 | 117 |
| MS.gene059051.t1 | MTR_2g035170 | 52.083 | 96 | 46 | 0 | 27 | 122 | 62 | 157 | 3.11e-29 | 103 |
| MS.gene059051.t1 | MTR_4g120940 | 65.753 | 73 | 25 | 0 | 50 | 122 | 47 | 119 | 1.11e-24 | 91.3 |
| MS.gene059051.t1 | MTR_4g120950 | 40.659 | 91 | 52 | 2 | 34 | 122 | 68 | 158 | 1.32e-14 | 66.2 |
| MS.gene059051.t1 | MTR_2g435310 | 23.718 | 156 | 83 | 1 | 1 | 120 | 1 | 156 | 8.01e-13 | 61.6 |
| MS.gene059051.t1 | MTR_4g120760 | 24.841 | 157 | 82 | 1 | 1 | 121 | 1 | 157 | 4.69e-12 | 59.7 |
| MS.gene059051.t1 | MTR_4g120760 | 24.841 | 157 | 82 | 1 | 1 | 121 | 1 | 157 | 5.00e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATACATTTACTTAATAATT+AGG | 0.132764 | 2.4:+55924722 | None:intergenic |
| GGCCATTGAGGGTTACGTTT+TGG | 0.315356 | 2.4:-55924750 | MS.gene059051:CDS |
| CTTGATAAGTCCAGTTCCTT+TGG | 0.329858 | 2.4:+55924772 | None:intergenic |
| ATACATTTACTTAATAATTA+GGG | 0.345110 | 2.4:+55924723 | None:intergenic |
| AGAGCTTTGTAGAGTTTAGC+TGG | 0.395172 | 2.4:+55925365 | None:intergenic |
| AGCAGTGGATGAGGCAAACT+TGG | 0.402684 | 2.4:-55924969 | MS.gene059051:CDS |
| TATAGCATAGTAGGAGGAAC+AGG | 0.403927 | 2.4:-55924938 | MS.gene059051:CDS |
| AGATAGAACTGCATCACCTT+TGG | 0.408990 | 2.4:+55924820 | None:intergenic |
| GAGACTAAGGCCAAAGGAAC+TGG | 0.415366 | 2.4:-55924782 | MS.gene059051:CDS |
| AGTGTTGTGGCTGGCTCTGA+TGG | 0.429803 | 2.4:-55924878 | MS.gene059051:CDS |
| GGATACAACTATAGCATAGT+AGG | 0.470749 | 2.4:-55924947 | MS.gene059051:CDS |
| GGACTTATCAAGGCCATTGA+GGG | 0.495298 | 2.4:-55924761 | MS.gene059051:CDS |
| TGCCAAAACGTAACCCTCAA+TGG | 0.499044 | 2.4:+55924748 | None:intergenic |
| CAAAGGAACTGGACTTATCA+AGG | 0.516806 | 2.4:-55924771 | MS.gene059051:CDS |
| CAAATTAGAAGCAGTGGATG+AGG | 0.520372 | 2.4:-55924978 | MS.gene059051:CDS |
| AGCAGTACGTGAGGAGACTA+AGG | 0.536209 | 2.4:-55924795 | MS.gene059051:CDS |
| TGGACTTATCAAGGCCATTG+AGG | 0.566375 | 2.4:-55924762 | MS.gene059051:CDS |
| TTTCACTGAAATCTTAACAA+TGG | 0.569031 | 2.4:+55924850 | None:intergenic |
| TAATTTGTGTAGCACAAAGT+CGG | 0.587472 | 2.4:+55924994 | None:intergenic |
| TTCGAGACAAGTGTTGTGGC+TGG | 0.589865 | 2.4:-55924887 | MS.gene059051:CDS |
| GTTGTGGCTGGCTCTGATGG+TGG | 0.598616 | 2.4:-55924875 | MS.gene059051:CDS |
| GCTACACAAATTAGAAGCAG+TGG | 0.603203 | 2.4:-55924984 | MS.gene059051:CDS |
| CAGTTGAGACATGTTCATCA+AGG | 0.603838 | 2.4:+55925393 | None:intergenic |
| CATAGTAGGAGGAACAGGGT+TGG | 0.607807 | 2.4:-55924933 | MS.gene059051:CDS |
| GCAGTGGATGAGGCAAACTT+GGG | 0.627060 | 2.4:-55924968 | MS.gene059051:CDS |
| TACAACTATAGCATAGTAGG+AGG | 0.648903 | 2.4:-55924944 | MS.gene059051:CDS |
| TGAATTCGAGACAAGTGTTG+TGG | 0.660471 | 2.4:-55924891 | MS.gene059051:CDS |
| TCAGTGAAATACCACACCAA+AGG | 0.661287 | 2.4:-55924836 | MS.gene059051:CDS |
| CGTGAGGAGACTAAGGCCAA+AGG | 0.670663 | 2.4:-55924788 | MS.gene059051:CDS |
| GAACTGCATCACCTTTGGTG+TGG | 0.674778 | 2.4:+55924825 | None:intergenic |
| TCTATCTGAAGCAGTACGTG+AGG | 0.678507 | 2.4:-55924804 | MS.gene059051:CDS |
| TGATGAACATGTCTCAACTG+TGG | 0.702962 | 2.4:-55925390 | MS.gene059051:CDS |
| ATAGCATAGTAGGAGGAACA+GGG | 0.716365 | 2.4:-55924937 | MS.gene059051:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAACTAAAACATTTACA+CGG | - | chr2.4:55924939-55924958 | MS.gene059051:CDS | 15.0% |
| !!! | TCAAACTTTATTTACATTTA+AGG | - | chr2.4:55925078-55925097 | MS.gene059051:intron | 15.0% |
| !! | CTAAAAATTATTTCAGTCAT+CGG | + | chr2.4:55925058-55925077 | None:intergenic | 20.0% |
| ! | ATTTATGATGTGTGTATTGT+AGG | - | chr2.4:55925118-55925137 | MS.gene059051:intron | 25.0% |
| ! | TTTCACTGAAATCTTAACAA+TGG | + | chr2.4:55925296-55925315 | None:intergenic | 25.0% |
| !!! | AACTGTTGTTTATGGTATTT+TGG | - | chr2.4:55924843-55924862 | MS.gene059051:CDS | 25.0% |
| !!! | ATGGTATTTTGGTATTTTTG+TGG | - | chr2.4:55924854-55924873 | MS.gene059051:CDS | 25.0% |
| !!! | TTACACGGTTGATTTTTTTT+AGG | - | chr2.4:55924954-55924973 | MS.gene059051:CDS | 25.0% |
| ! | AATGTTAACTGTTGTTGTTG+TGG | - | chr2.4:55924806-55924825 | MS.gene059051:CDS | 30.0% |
| ! | TAATTTGTGTAGCACAAAGT+CGG | + | chr2.4:55925152-55925171 | None:intergenic | 30.0% |
| GGATACAACTATAGCATAGT+AGG | - | chr2.4:55925196-55925215 | MS.gene059051:intron | 35.0% | |
| TACAACTATAGCATAGTAGG+AGG | - | chr2.4:55925199-55925218 | MS.gene059051:intron | 35.0% | |
| TATGATGTGTGTATTGTAGG+TGG | - | chr2.4:55925121-55925140 | MS.gene059051:intron | 35.0% | |
| ! | AACGATATCCAGTTACTTTC+AGG | - | chr2.4:55925028-55925047 | MS.gene059051:intron | 35.0% |
| ! | CGACAGTTAACTGTTGTTTA+TGG | - | chr2.4:55924835-55924854 | MS.gene059051:CDS | 35.0% |
| ! | TTAGGACTAGAGTTGATTGT+TGG | - | chr2.4:55924972-55924991 | MS.gene059051:CDS | 35.0% |
| AGATAGAACTGCATCACCTT+TGG | + | chr2.4:55925326-55925345 | None:intergenic | 40.0% | |
| ATAGCATAGTAGGAGGAACA+GGG | - | chr2.4:55925206-55925225 | MS.gene059051:intron | 40.0% | |
| CAAAGGAACTGGACTTATCA+AGG | - | chr2.4:55925372-55925391 | MS.gene059051:CDS | 40.0% | |
| CAAATTAGAAGCAGTGGATG+AGG | - | chr2.4:55925165-55925184 | MS.gene059051:intron | 40.0% | |
| CAGTTGAGACATGTTCATCA+AGG | + | chr2.4:55924753-55924772 | None:intergenic | 40.0% | |
| CTTGATAAGTCCAGTTCCTT+TGG | + | chr2.4:55925374-55925393 | None:intergenic | 40.0% | |
| GCTACACAAATTAGAAGCAG+TGG | - | chr2.4:55925159-55925178 | MS.gene059051:intron | 40.0% | |
| TATAGCATAGTAGGAGGAAC+AGG | - | chr2.4:55925205-55925224 | MS.gene059051:intron | 40.0% | |
| TCAGTGAAATACCACACCAA+AGG | - | chr2.4:55925307-55925326 | MS.gene059051:intron | 40.0% | |
| TGATGAACATGTCTCAACTG+TGG | - | chr2.4:55924753-55924772 | MS.gene059051:CDS | 40.0% | |
| ! | AGAGCTTTGTAGAGTTTAGC+TGG | + | chr2.4:55924781-55924800 | None:intergenic | 40.0% |
| ! | TGAATTCGAGACAAGTGTTG+TGG | - | chr2.4:55925252-55925271 | MS.gene059051:intron | 40.0% |
| GGACTTATCAAGGCCATTGA+GGG | - | chr2.4:55925382-55925401 | MS.gene059051:CDS | 45.0% | |
| TCGGTTGACCTGAAAGTAAC+TGG | + | chr2.4:55925039-55925058 | None:intergenic | 45.0% | |
| TGCCAAAACGTAACCCTCAA+TGG | + | chr2.4:55925398-55925417 | None:intergenic | 45.0% | |
| TGGACTTATCAAGGCCATTG+AGG | - | chr2.4:55925381-55925400 | MS.gene059051:CDS | 45.0% | |
| ! | TCTATCTGAAGCAGTACGTG+AGG | - | chr2.4:55925339-55925358 | MS.gene059051:intron | 45.0% |
| AGCAGTACGTGAGGAGACTA+AGG | - | chr2.4:55925348-55925367 | MS.gene059051:intron | 50.0% | |
| AGCAGTGGATGAGGCAAACT+TGG | - | chr2.4:55925174-55925193 | MS.gene059051:intron | 50.0% | |
| CATAGTAGGAGGAACAGGGT+TGG | - | chr2.4:55925210-55925229 | MS.gene059051:intron | 50.0% | |
| GAACTGCATCACCTTTGGTG+TGG | + | chr2.4:55925321-55925340 | None:intergenic | 50.0% | |
| GAGACTAAGGCCAAAGGAAC+TGG | - | chr2.4:55925361-55925380 | MS.gene059051:CDS | 50.0% | |
| GCAGTGGATGAGGCAAACTT+GGG | - | chr2.4:55925175-55925194 | MS.gene059051:intron | 50.0% | |
| ! | GGCCATTGAGGGTTACGTTT+TGG | - | chr2.4:55925393-55925412 | MS.gene059051:CDS | 50.0% |
| ! | TTCGAGACAAGTGTTGTGGC+TGG | - | chr2.4:55925256-55925275 | MS.gene059051:intron | 50.0% |
| CGTGAGGAGACTAAGGCCAA+AGG | - | chr2.4:55925355-55925374 | MS.gene059051:intron | 55.0% | |
| !! | AGTGTTGTGGCTGGCTCTGA+TGG | - | chr2.4:55925265-55925284 | MS.gene059051:intron | 55.0% |
| !! | GTTGTGGCTGGCTCTGATGG+TGG | - | chr2.4:55925268-55925287 | MS.gene059051:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 55924733 | 55925432 | 55924733 | ID=MS.gene059051 |
| chr2.4 | mRNA | 55924733 | 55925432 | 55924733 | ID=MS.gene059051.t1;Parent=MS.gene059051 |
| chr2.4 | exon | 55925357 | 55925432 | 55925357 | ID=MS.gene059051.t1.exon1;Parent=MS.gene059051.t1 |
| chr2.4 | CDS | 55925357 | 55925432 | 55925357 | ID=cds.MS.gene059051.t1;Parent=MS.gene059051.t1 |
| chr2.4 | exon | 55924733 | 55925025 | 55924733 | ID=MS.gene059051.t1.exon2;Parent=MS.gene059051.t1 |
| chr2.4 | CDS | 55924733 | 55925025 | 55924733 | ID=cds.MS.gene059051.t1;Parent=MS.gene059051.t1 |
| Gene Sequence |
| Protein sequence |
