Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86584.t1 | XP_013452555.1 | 95.9 | 242 | 10 | 0 | 1 | 242 | 1 | 242 | 3.00E-124 | 454.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86584.t1 | G7ZX37 | 95.9 | 242 | 10 | 0 | 1 | 242 | 1 | 242 | 2.2e-124 | 454.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene86584 | 0.801468 | 8.88E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene86584 | 0.818294 | 2.11E-52 | -1.69E-46 |
| MS.gene05105 | MS.gene86584 | 0.80183 | 7.48E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene86584 | 0.826115 | 3.24E-54 | -1.69E-46 |
| MS.gene052253 | MS.gene86584 | 0.808848 | 2.53E-50 | -1.69E-46 |
| MS.gene052310 | MS.gene86584 | 0.810622 | 1.05E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene86584 | 0.826469 | 2.67E-54 | -1.69E-46 |
| MS.gene053209 | MS.gene86584 | 0.800862 | 1.18E-48 | -1.69E-46 |
| MS.gene053557 | MS.gene86584 | 0.805374 | 1.38E-49 | -1.69E-46 |
| MS.gene055116 | MS.gene86584 | 0.813623 | 2.33E-51 | -1.69E-46 |
| MS.gene056659 | MS.gene86584 | 0.804962 | 1.68E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene86584 | 0.801378 | 9.26E-49 | -1.69E-46 |
| MS.gene058082 | MS.gene86584 | 0.80287 | 4.57E-49 | -1.69E-46 |
| MS.gene05811 | MS.gene86584 | 0.803118 | 4.06E-49 | -1.69E-46 |
| MS.gene059285 | MS.gene86584 | 0.825527 | 4.47E-54 | -1.69E-46 |
| MS.gene059366 | MS.gene86584 | 0.805101 | 1.57E-49 | -1.69E-46 |
| MS.gene059688 | MS.gene86584 | 0.805127 | 1.55E-49 | -1.69E-46 |
| MS.gene059692 | MS.gene86584 | 0.813802 | 2.13E-51 | -1.69E-46 |
| MS.gene059695 | MS.gene86584 | 0.813224 | 2.85E-51 | -1.69E-46 |
| MS.gene059807 | MS.gene86584 | 0.80248 | 5.50E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene86584 | 0.808207 | 3.47E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene86584 | 0.803025 | 4.24E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene86584 | 0.817797 | 2.74E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86584.t1 | MTR_6g465290 | 95.868 | 242 | 10 | 0 | 1 | 242 | 1 | 242 | 9.86e-170 | 468 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86584.t1 | AT5G62140 | 58.768 | 211 | 79 | 3 | 37 | 239 | 27 | 237 | 2.32e-79 | 239 |
Find 40 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATGGAGTTTCAATTGTTT+TGG | 0.254521 | 6.1:+51367578 | None:intergenic |
| ATACTTCCTTGTAAAGCTTC+TGG | 0.265557 | 6.1:+51367300 | None:intergenic |
| AGCTCTACAACAAAGTTTCT+TGG | 0.268474 | 6.1:-51367360 | MS.gene86584:CDS |
| GGAGGAATAGGAACTGGTTT+TGG | 0.356740 | 6.1:-51367465 | MS.gene86584:CDS |
| GTGTAAGCATTATCATTTGA+TGG | 0.361969 | 6.1:+51367561 | None:intergenic |
| CTCTTATGATGAAGAAGAAA+TGG | 0.372803 | 6.1:+51365988 | None:intergenic |
| GGAAGTATCAATCAAGAATC+TGG | 0.380544 | 6.1:-51367285 | MS.gene86584:CDS |
| TTCTGCCGGTAGTATCTATA+AGG | 0.385179 | 6.1:-51366212 | MS.gene86584:CDS |
| TTCCTAATGAATTCCTTCCT+TGG | 0.410690 | 6.1:-51366052 | MS.gene86584:CDS |
| TATGATGCTGAAGGAGGAAT+AGG | 0.412723 | 6.1:-51367477 | MS.gene86584:CDS |
| CAAGGTTGATCTTGAGTTCA+AGG | 0.421322 | 6.1:-51366248 | MS.gene86584:intron |
| GACCAAGGAAGGAATTCATT+AGG | 0.428876 | 6.1:+51366050 | None:intergenic |
| TCAGCATCATACTCAAAATC+AGG | 0.441137 | 6.1:+51367489 | None:intergenic |
| ATATGAAATTTGCATTGTAT+TGG | 0.443194 | 6.1:+51367681 | None:intergenic |
| TGAAATTTGCATTGTATTGG+TGG | 0.454546 | 6.1:+51367684 | None:intergenic |
| GAGTGGAAGAGGCATGAGAT+TGG | 0.458016 | 6.1:-51366131 | MS.gene86584:CDS |
| GTTGTAGAGCTTGTTAATGA+TGG | 0.460648 | 6.1:+51367372 | None:intergenic |
| AGGCATGAGATTGGATGAAG+AGG | 0.462908 | 6.1:-51366122 | MS.gene86584:CDS |
| TTCATTAGGAAATCATCAAT+AGG | 0.476929 | 6.1:+51366064 | None:intergenic |
| GCTGAAGGAGGAATAGGAAC+TGG | 0.519626 | 6.1:-51367471 | MS.gene86584:CDS |
| GGCATGAGATTGGATGAAGA+GGG | 0.522269 | 6.1:-51366121 | MS.gene86584:CDS |
| ACCGGCAGAAAACAAGAACT+TGG | 0.533026 | 6.1:+51366225 | None:intergenic |
| TTGACATCTGAAGAATCAAA+GGG | 0.535919 | 6.1:-51366163 | MS.gene86584:CDS |
| GGGACTATGAAGAGTGGAAG+AGG | 0.554975 | 6.1:-51366142 | MS.gene86584:CDS |
| GTTGACATCTGAAGAATCAA+AGG | 0.561822 | 6.1:-51366164 | MS.gene86584:CDS |
| CTCAGTAGGTAGACCAAGGA+AGG | 0.566579 | 6.1:+51366039 | None:intergenic |
| ATTGTTCCAGAAGCTTTACA+AGG | 0.575593 | 6.1:-51367306 | MS.gene86584:CDS |
| AACACTGTCTTCACAAGTAG+TGG | 0.578508 | 6.1:+51366184 | None:intergenic |
| AAAGTGCAGGCTGGTTGGTG+TGG | 0.587134 | 6.1:-51366098 | MS.gene86584:CDS |
| AGTTCAGCAAGACACTCAGT+AGG | 0.604330 | 6.1:+51366025 | None:intergenic |
| GAGGGAAAGTGCAGGCTGGT+TGG | 0.614866 | 6.1:-51366103 | MS.gene86584:CDS |
| TTTGAGTATGATGCTGAAGG+AGG | 0.619744 | 6.1:-51367483 | MS.gene86584:CDS |
| TGGAGCCTTATAGATACTAC+CGG | 0.628902 | 6.1:+51366207 | None:intergenic |
| TGAAGAGGGAAAGTGCAGGC+TGG | 0.630215 | 6.1:-51366107 | MS.gene86584:CDS |
| TCAAAGGGGACTATGAAGAG+TGG | 0.635845 | 6.1:-51366148 | MS.gene86584:CDS |
| GACACTCAGTAGGTAGACCA+AGG | 0.644039 | 6.1:+51366035 | None:intergenic |
| ACTGTCTTCACAAGTAGTGG+TGG | 0.644274 | 6.1:+51366187 | None:intergenic |
| TATCAATCAAGAATCTGGCA+AGG | 0.648241 | 6.1:-51367280 | MS.gene86584:intron |
| TGGATGAAGAGGGAAAGTGC+AGG | 0.678599 | 6.1:-51366111 | MS.gene86584:CDS |
| TGACATCTGAAGAATCAAAG+GGG | 0.752025 | 6.1:-51366162 | MS.gene86584:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACTAAGAAAAGATAAAATT+AGG | + | chr6.1:51367059-51367078 | None:intergenic | 15.0% |
| !!! | TTTTTCCTTTAATTATTACT+CGG | - | chr6.1:51366690-51366709 | MS.gene86584:intron | 15.0% |
| !! | AAAATATTTAAGGGATACAA+TGG | + | chr6.1:51366540-51366559 | None:intergenic | 20.0% |
| !! | ATATGAAATTTGCATTGTAT+TGG | + | chr6.1:51366024-51366043 | None:intergenic | 20.0% |
| !!! | AGCATTTTCCAAAATATTTA+AGG | + | chr6.1:51366550-51366569 | None:intergenic | 20.0% |
| !!! | ATTGTATCCCTTAAATATTT+TGG | - | chr6.1:51366539-51366558 | MS.gene86584:intron | 20.0% |
| !!! | GCATTTTCCAAAATATTTAA+GGG | + | chr6.1:51366549-51366568 | None:intergenic | 20.0% |
| !!! | TCATTTTCACAAAATTTTAC+CGG | - | chr6.1:51367346-51367365 | MS.gene86584:CDS | 20.0% |
| !!! | TGTTTTGTAATTTTGATATG+AGG | - | chr6.1:51367188-51367207 | MS.gene86584:intron | 20.0% |
| ! | ACAATATCAATCCTCAAAAA+TGG | + | chr6.1:51366381-51366400 | None:intergenic | 25.0% |
| ! | CTGACACAACATATATATAT+CGG | + | chr6.1:51367280-51367299 | None:intergenic | 25.0% |
| ! | GTTACTATAATATCAAGAGA+CGG | - | chr6.1:51366862-51366881 | MS.gene86584:intron | 25.0% |
| ! | TTCATTAGGAAATCATCAAT+AGG | + | chr6.1:51367641-51367660 | None:intergenic | 25.0% |
| !! | AAACACAAAGTGTTACTAAA+AGG | + | chr6.1:51366506-51366525 | None:intergenic | 25.0% |
| !!! | AGTTTCAATTGTTTTGGAAA+TGG | + | chr6.1:51366121-51366140 | None:intergenic | 25.0% |
| ATATCAATCCTCAAAAATGG+TGG | + | chr6.1:51366378-51366397 | None:intergenic | 30.0% | |
| ATGAAGAAAAGAACGTTTAG+GGG | - | chr6.1:51367165-51367184 | MS.gene86584:intron | 30.0% | |
| ATTTAAGGGATACAATGGTT+AGG | + | chr6.1:51366535-51366554 | None:intergenic | 30.0% | |
| CATGAAGAAAAGAACGTTTA+GGG | - | chr6.1:51367164-51367183 | MS.gene86584:intron | 30.0% | |
| GAGTCAAATCCAATAGTTAA+TGG | - | chr6.1:51367210-51367229 | MS.gene86584:intron | 30.0% | |
| GTGTAAGCATTATCATTTGA+TGG | + | chr6.1:51366144-51366163 | None:intergenic | 30.0% | |
| TCATGAAGAAAAGAACGTTT+AGG | - | chr6.1:51367163-51367182 | MS.gene86584:intron | 30.0% | |
| TGAAATTTGCATTGTATTGG+TGG | + | chr6.1:51366021-51366040 | None:intergenic | 30.0% | |
| TTCCTTTAATTATTACTCGG+AGG | - | chr6.1:51366693-51366712 | MS.gene86584:intron | 30.0% | |
| TTCTTAAAACCAATAACCAG+AGG | + | chr6.1:51366953-51366972 | None:intergenic | 30.0% | |
| TTGACATCTGAAGAATCAAA+GGG | - | chr6.1:51367539-51367558 | MS.gene86584:CDS | 30.0% | |
| ! | TGATGGAGTTTCAATTGTTT+TGG | + | chr6.1:51366127-51366146 | None:intergenic | 30.0% |
| !!! | TTGTGTTGTTTTTCACTTGA+GGG | + | chr6.1:51366096-51366115 | None:intergenic | 30.0% |
| !!! | TTTGTGTTGTTTTTCACTTG+AGG | + | chr6.1:51366097-51366116 | None:intergenic | 30.0% |
| AAATGCTAACTAGAACTCCA+AGG | - | chr6.1:51366563-51366582 | MS.gene86584:intron | 35.0% | |
| AATGCTAACTAGAACTCCAA+GGG | - | chr6.1:51366564-51366583 | MS.gene86584:intron | 35.0% | |
| ACATATATATATCGGACACC+TGG | + | chr6.1:51367272-51367291 | None:intergenic | 35.0% | |
| AGCTCTACAACAAAGTTTCT+TGG | - | chr6.1:51366342-51366361 | MS.gene86584:intron | 35.0% | |
| AGTCAAAATCCTCTGGTTAT+TGG | - | chr6.1:51366941-51366960 | MS.gene86584:intron | 35.0% | |
| ATACTTCCTTGTAAAGCTTC+TGG | + | chr6.1:51366405-51366424 | None:intergenic | 35.0% | |
| ATTGTTCCAGAAGCTTTACA+AGG | - | chr6.1:51366396-51366415 | MS.gene86584:intron | 35.0% | |
| CAACAGAGTCCATTAACTAT+TGG | + | chr6.1:51367222-51367241 | None:intergenic | 35.0% | |
| GACTAAACTAACGAACGAAA+GGG | - | chr6.1:51367123-51367142 | MS.gene86584:intron | 35.0% | |
| GAGTTTGACCCTGTAATATT+AGG | - | chr6.1:51366912-51366931 | MS.gene86584:intron | 35.0% | |
| GCAACGATATCTCAATTTGA+TGG | + | chr6.1:51366753-51366772 | None:intergenic | 35.0% | |
| GTCCTCCGAGTAATAATTAA+AGG | + | chr6.1:51366698-51366717 | None:intergenic | 35.0% | |
| GTTGACATCTGAAGAATCAA+AGG | - | chr6.1:51367538-51367557 | MS.gene86584:CDS | 35.0% | |
| TACCACTGATACTGGAAATT+TGG | + | chr6.1:51366891-51366910 | None:intergenic | 35.0% | |
| TATCAATCAAGAATCTGGCA+AGG | - | chr6.1:51366422-51366441 | MS.gene86584:intron | 35.0% | |
| TCAGCATCATACTCAAAATC+AGG | + | chr6.1:51366216-51366235 | None:intergenic | 35.0% | |
| TGACATCTGAAGAATCAAAG+GGG | - | chr6.1:51367540-51367559 | MS.gene86584:CDS | 35.0% | |
| TGACTAAACTAACGAACGAA+AGG | - | chr6.1:51367122-51367141 | MS.gene86584:intron | 35.0% | |
| TGTTCGAGCTTCATAGATAA+TGG | - | chr6.1:51367407-51367426 | MS.gene86584:CDS | 35.0% | |
| TTCCTAATGAATTCCTTCCT+TGG | - | chr6.1:51367650-51367669 | MS.gene86584:CDS | 35.0% | |
| !! | GATTTTGAGTATGATGCTGA+AGG | - | chr6.1:51366216-51366235 | MS.gene86584:CDS | 35.0% |
| !! | GGAAGTATCAATCAAGAATC+TGG | - | chr6.1:51366417-51366436 | MS.gene86584:intron | 35.0% |
| !! | GTTGTAGAGCTTGTTAATGA+TGG | + | chr6.1:51366333-51366352 | None:intergenic | 35.0% |
| AACACTGTCTTCACAAGTAG+TGG | + | chr6.1:51367521-51367540 | None:intergenic | 40.0% | |
| AACTCCAAGGGAATCAGTTA+AGG | - | chr6.1:51366576-51366595 | MS.gene86584:intron | 40.0% | |
| ACTCCCTCTCCTAATATTAC+AGG | + | chr6.1:51366924-51366943 | None:intergenic | 40.0% | |
| CAAACTCTTACCACTGATAC+TGG | + | chr6.1:51366899-51366918 | None:intergenic | 40.0% | |
| CTCCCTCTCCTAATATTACA+GGG | + | chr6.1:51366923-51366942 | None:intergenic | 40.0% | |
| GACCAAGGAAGGAATTCATT+AGG | + | chr6.1:51367655-51367674 | None:intergenic | 40.0% | |
| GACCCTGTAATATTAGGAGA+GGG | - | chr6.1:51366918-51366937 | MS.gene86584:intron | 40.0% | |
| GTCCAAATTTCCAGTATCAG+TGG | - | chr6.1:51366886-51366905 | MS.gene86584:intron | 40.0% | |
| TATGATGCTGAAGGAGGAAT+AGG | - | chr6.1:51366225-51366244 | MS.gene86584:CDS | 40.0% | |
| TGACCCTGTAATATTAGGAG+AGG | - | chr6.1:51366917-51366936 | MS.gene86584:intron | 40.0% | |
| TTCTGATTGAAAACGTGTCC+AGG | - | chr6.1:51367251-51367270 | MS.gene86584:intron | 40.0% | |
| TTCTGCCGGTAGTATCTATA+AGG | - | chr6.1:51367490-51367509 | MS.gene86584:CDS | 40.0% | |
| TTTGAGTATGATGCTGAAGG+AGG | - | chr6.1:51366219-51366238 | MS.gene86584:CDS | 40.0% | |
| ! | CAAGGTTGATCTTGAGTTCA+AGG | - | chr6.1:51367454-51367473 | MS.gene86584:CDS | 40.0% |
| ! | GTTTCCTTAACTGATTCCCT+TGG | + | chr6.1:51366583-51366602 | None:intergenic | 40.0% |
| ! | TGGAGCCTTATAGATACTAC+CGG | + | chr6.1:51367498-51367517 | None:intergenic | 40.0% |
| !! | ACAAAAAGTGCTTGCAAGCT+TGG | - | chr6.1:51366177-51366196 | MS.gene86584:CDS | 40.0% |
| !! | AGTTAATGGACTCTGTTGCA+CGG | - | chr6.1:51367224-51367243 | MS.gene86584:intron | 40.0% |
| !! | TGTCATTGCCACCATTTTTG+AGG | - | chr6.1:51366367-51366386 | MS.gene86584:intron | 40.0% |
| ACAGACATCGAAACACGACA+CGG | + | chr6.1:51367382-51367401 | None:intergenic | 45.0% | |
| ACCGGCAGAAAACAAGAACT+TGG | + | chr6.1:51367480-51367499 | None:intergenic | 45.0% | |
| ACTGTCTTCACAAGTAGTGG+TGG | + | chr6.1:51367518-51367537 | None:intergenic | 45.0% | |
| AGGCATGAGATTGGATGAAG+AGG | - | chr6.1:51367580-51367599 | MS.gene86584:CDS | 45.0% | |
| AGTTCAGCAAGACACTCAGT+AGG | + | chr6.1:51367680-51367699 | None:intergenic | 45.0% | |
| CACAAGTGTCAGTATCGTGT+CGG | + | chr6.1:51367306-51367325 | None:intergenic | 45.0% | |
| GGCATGAGATTGGATGAAGA+GGG | - | chr6.1:51367581-51367600 | MS.gene86584:CDS | 45.0% | |
| TCAAAGGGGACTATGAAGAG+TGG | - | chr6.1:51367554-51367573 | MS.gene86584:CDS | 45.0% | |
| !! | GGAGGAATAGGAACTGGTTT+TGG | - | chr6.1:51366237-51366256 | MS.gene86584:CDS | 45.0% |
| !!! | GCCAAGTTCTTGTTTTCTGC+CGG | - | chr6.1:51367476-51367495 | MS.gene86584:CDS | 45.0% |
| !!! | AATTTTTATTTTTTATTACA+AGG | - | chr6.1:51367436-51367455 | MS.gene86584:CDS | 5.0% |
| ACGACACGGACATTACACAC+CGG | + | chr6.1:51367368-51367387 | None:intergenic | 50.0% | |
| CTCAGTAGGTAGACCAAGGA+AGG | + | chr6.1:51367666-51367685 | None:intergenic | 50.0% | |
| GACACTCAGTAGGTAGACCA+AGG | + | chr6.1:51367670-51367689 | None:intergenic | 50.0% | |
| GAGAGGGAGTCAAAATCCTC+TGG | - | chr6.1:51366934-51366953 | MS.gene86584:intron | 50.0% | |
| GCTGAAGGAGGAATAGGAAC+TGG | - | chr6.1:51366231-51366250 | MS.gene86584:CDS | 50.0% | |
| GGGACTATGAAGAGTGGAAG+AGG | - | chr6.1:51367560-51367579 | MS.gene86584:CDS | 50.0% | |
| ! | GAGTGGAAGAGGCATGAGAT+TGG | - | chr6.1:51367571-51367590 | MS.gene86584:CDS | 50.0% |
| ! | TGGATGAAGAGGGAAAGTGC+AGG | - | chr6.1:51367591-51367610 | MS.gene86584:CDS | 50.0% |
| ! | AAAGTGCAGGCTGGTTGGTG+TGG | - | chr6.1:51367604-51367623 | MS.gene86584:CDS | 55.0% |
| ! | TGAAGAGGGAAAGTGCAGGC+TGG | - | chr6.1:51367595-51367614 | MS.gene86584:CDS | 55.0% |
| ! | GAGGGAAAGTGCAGGCTGGT+TGG | - | chr6.1:51367599-51367618 | MS.gene86584:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 51365991 | 51367733 | 51365991 | ID=MS.gene86584 |
| chr6.1 | mRNA | 51365991 | 51367733 | 51365991 | ID=MS.gene86584.t1;Parent=MS.gene86584 |
| chr6.1 | exon | 51367281 | 51367733 | 51367281 | ID=MS.gene86584.t1.exon1;Parent=MS.gene86584.t1 |
| chr6.1 | CDS | 51367281 | 51367733 | 51367281 | ID=cds.MS.gene86584.t1;Parent=MS.gene86584.t1 |
| chr6.1 | exon | 51365991 | 51366266 | 51365991 | ID=MS.gene86584.t1.exon2;Parent=MS.gene86584.t1 |
| chr6.1 | CDS | 51365991 | 51366266 | 51365991 | ID=cds.MS.gene86584.t1;Parent=MS.gene86584.t1 |
| Gene Sequence |
| Protein sequence |
