Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86822.t1 | XP_013468867.1 | 99.8 | 419 | 1 | 0 | 1 | 419 | 102 | 520 | 6.30E-202 | 713.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86822.t1 | Q9FHY8 | 69.4 | 421 | 125 | 3 | 1 | 419 | 147 | 565 | 2.3e-128 | 460.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86822.t1 | A0A072VLP9 | 99.8 | 419 | 1 | 0 | 1 | 419 | 102 | 520 | 4.5e-202 | 713.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050838 | MS.gene86822 | 0.881153 | 3.04E-70 | -1.69E-46 |
| MS.gene051003 | MS.gene86822 | 0.828083 | 1.09E-54 | -1.69E-46 |
| MS.gene051234 | MS.gene86822 | 0.849596 | 2.92E-60 | -1.69E-46 |
| MS.gene051343 | MS.gene86822 | 0.80634 | 8.63E-50 | -1.69E-46 |
| MS.gene051381 | MS.gene86822 | 0.814472 | 1.51E-51 | -1.69E-46 |
| MS.gene051642 | MS.gene86822 | 0.813535 | 2.44E-51 | -1.69E-46 |
| MS.gene051885 | MS.gene86822 | 0.814816 | 1.27E-51 | -1.69E-46 |
| MS.gene052072 | MS.gene86822 | 0.80839 | 3.17E-50 | -1.69E-46 |
| MS.gene05224 | MS.gene86822 | 0.803199 | 3.91E-49 | -1.69E-46 |
| MS.gene052307 | MS.gene86822 | 0.81361 | 2.35E-51 | -1.69E-46 |
| MS.gene052609 | MS.gene86822 | 0.81433 | 1.63E-51 | -1.69E-46 |
| MS.gene053143 | MS.gene86822 | 0.82853 | 8.54E-55 | -1.69E-46 |
| MS.gene055513 | MS.gene86822 | 0.801505 | 8.73E-49 | -1.69E-46 |
| MS.gene056181 | MS.gene86822 | 0.806965 | 6.37E-50 | -1.69E-46 |
| MS.gene05880 | MS.gene86822 | 0.820908 | 5.35E-53 | -1.69E-46 |
| MS.gene059171 | MS.gene86822 | 0.809036 | 2.31E-50 | -1.69E-46 |
| MS.gene059227 | MS.gene86822 | 0.812585 | 3.94E-51 | -1.69E-46 |
| MS.gene059778 | MS.gene86822 | 0.813173 | 2.93E-51 | -1.69E-46 |
| MS.gene061006 | MS.gene86822 | 0.817762 | 2.79E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene86822 | 0.845014 | 5.29E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86822.t1 | MTR_1g077850 | 99.523 | 419 | 2 | 0 | 1 | 419 | 102 | 520 | 0.0 | 865 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86822.t1 | AT5G41950 | 75.414 | 423 | 96 | 4 | 1 | 419 | 147 | 565 | 0.0 | 657 |
Find 105 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTTCAGGCGCTTAATAAT+TGG | 0.142305 | 1.1:-58289014 | MS.gene86822:intron |
| GAGCTAACATCTTCTCTTTC+GGG | 0.200894 | 1.1:+58293128 | None:intergenic |
| CGGTTAAATATCCAACTTTA+AGG | 0.206145 | 1.1:+58286059 | None:intergenic |
| TGGCTTCTTACTGAATAATT+TGG | 0.228506 | 1.1:+58286736 | None:intergenic |
| CACCGGGCAATTTACAATCT+TGG | 0.286151 | 1.1:-58287007 | MS.gene86822:CDS |
| TACAGTGCATCATAATCTTC+AGG | 0.287071 | 1.1:+58291989 | None:intergenic |
| AAGATGTTAGCTCTCCTTAC+AGG | 0.291498 | 1.1:-58293118 | MS.gene86822:intron |
| ATTTCAGGCGCTTAATAATT+GGG | 0.322345 | 1.1:-58289013 | MS.gene86822:intron |
| AGAGCTAACATCTTCTCTTT+CGG | 0.335566 | 1.1:+58293127 | None:intergenic |
| TAATTGGGGACTTGCGCTTC+AGG | 0.337641 | 1.1:-58288998 | MS.gene86822:intron |
| TGCTGACTCTTGGGAATCAT+TGG | 0.349651 | 1.1:-58284989 | MS.gene86822:CDS |
| TTACAATCTTGGAACTGTTC+TGG | 0.353539 | 1.1:-58286996 | MS.gene86822:intron |
| ATTATGATGCACTGTACAAT+TGG | 0.360131 | 1.1:-58291982 | MS.gene86822:CDS |
| CTGACTTTGCCACCTGGTGC+TGG | 0.360992 | 1.1:-58285691 | MS.gene86822:CDS |
| GATTGGCTGTACAATTCATT+TGG | 0.366257 | 1.1:+58286793 | None:intergenic |
| CAATTGGGCATTGGTACTTC+AGG | 0.369935 | 1.1:-58291966 | MS.gene86822:intron |
| GCTGTTGCTGTTGACTATCT+TGG | 0.373402 | 1.1:+58292314 | None:intergenic |
| TTAGAAGGTGAAGTGGAATC+TGG | 0.383082 | 1.1:+58290452 | None:intergenic |
| ACCATGAGAGAATTGCTTCA+TGG | 0.392969 | 1.1:-58293170 | MS.gene86822:CDS |
| GCAGAGGACACTTTAAGAAC+AGG | 0.403120 | 1.1:-58286844 | MS.gene86822:CDS |
| TGCTTGCCTCTGTTCAACTC+TGG | 0.404309 | 1.1:+58285816 | None:intergenic |
| TTATCAAGGTTGCTGACTCT+TGG | 0.405651 | 1.1:-58284999 | MS.gene86822:intron |
| CTTGCGGTTGGTACGATCTA+TGG | 0.407275 | 1.1:-58286518 | MS.gene86822:intron |
| TTAAAGCAGTCCAACTCAAC+TGG | 0.408233 | 1.1:-58289250 | MS.gene86822:CDS |
| GTATCCAGGCTTACTACAAT+TGG | 0.408555 | 1.1:-58289586 | MS.gene86822:intron |
| ATTCATGGCATCTCCTGATC+TGG | 0.410632 | 1.1:+58285777 | None:intergenic |
| TCAAGCAGAGCATCTTTAGA+AGG | 0.411824 | 1.1:+58290437 | None:intergenic |
| CAAAGTCTCTCAACCAGATC+AGG | 0.413385 | 1.1:-58285790 | MS.gene86822:CDS |
| TATCAAGGTTGCTGACTCTT+GGG | 0.414988 | 1.1:-58284998 | MS.gene86822:intron |
| CTCTGCATTGATACAGTTCA+TGG | 0.422436 | 1.1:-58285667 | MS.gene86822:CDS |
| AGCTGAAGAGCTATGGAAAC+AGG | 0.425760 | 1.1:-58289516 | MS.gene86822:intron |
| GTATTGGCGGGTATTATAAC+AGG | 0.435968 | 1.1:-58284907 | MS.gene86822:CDS |
| CCGCTACCACACCTTAAAGT+TGG | 0.441526 | 1.1:-58286070 | MS.gene86822:CDS |
| GATCAGGAGATGCCATGAAT+GGG | 0.443681 | 1.1:-58285774 | MS.gene86822:CDS |
| GGAATCATTGGATGGATGGC+TGG | 0.453718 | 1.1:-58284977 | MS.gene86822:CDS |
| AGATCAGGAGATGCCATGAA+TGG | 0.456962 | 1.1:-58285775 | MS.gene86822:CDS |
| TATTTAACCGCACCTCCTGC+GGG | 0.458286 | 1.1:-58286046 | MS.gene86822:CDS |
| GATCGTTTCCAGTCATTATG+AGG | 0.464366 | 1.1:+58286010 | None:intergenic |
| AGTTCATGGATCAGTTTACT+TGG | 0.466595 | 1.1:-58285653 | MS.gene86822:intron |
| GAGTTGATAAACAGCGTTGT+AGG | 0.474591 | 1.1:-58292265 | MS.gene86822:CDS |
| ACTCAACTGGAACAGTCCTC+AGG | 0.484214 | 1.1:-58289237 | MS.gene86822:intron |
| AGTTGCAATTTGATTTCCAC+CGG | 0.493039 | 1.1:-58287024 | MS.gene86822:CDS |
| ATATTTAACCGCACCTCCTG+CGG | 0.501162 | 1.1:-58286047 | MS.gene86822:CDS |
| GCTTGCCTCTGTTCAACTCT+GGG | 0.504813 | 1.1:+58285817 | None:intergenic |
| CAAAGGAAGCTGAAGAGCTA+TGG | 0.504826 | 1.1:-58289523 | MS.gene86822:CDS |
| GGATCACGAAAAGCTTCAGC+AGG | 0.507858 | 1.1:-58285975 | MS.gene86822:intron |
| CTTGGGAATCATTGGATGGA+TGG | 0.512023 | 1.1:-58284981 | MS.gene86822:CDS |
| CTGCAATATATATTGCAGAT+TGG | 0.512331 | 1.1:+58286776 | None:intergenic |
| ACAGTGCATCATAATCTTCA+GGG | 0.517083 | 1.1:+58291990 | None:intergenic |
| TTCCAAGATTGTAAATTGCC+CGG | 0.521530 | 1.1:+58287005 | None:intergenic |
| GCTGATCTGACTTTGCCACC+TGG | 0.523003 | 1.1:-58285697 | MS.gene86822:CDS |
| GAGGACTGTTCCAGTTGAGT+TGG | 0.523242 | 1.1:+58289240 | None:intergenic |
| GAAATCAAATTGCAACTGTA+TGG | 0.525917 | 1.1:+58287030 | None:intergenic |
| TATCCAGGCTTACTACAATT+GGG | 0.527161 | 1.1:-58289585 | MS.gene86822:intron |
| AGAACAGAACAATGCTGCTA+TGG | 0.535089 | 1.1:-58292287 | MS.gene86822:CDS |
| TTGATGAGGAGGGACGGTCT+AGG | 0.539601 | 1.1:-58292240 | MS.gene86822:CDS |
| TTATGATGCACTGTACAATT+GGG | 0.539658 | 1.1:-58291981 | MS.gene86822:CDS |
| TTTCAGGCGCTTAATAATTG+GGG | 0.541226 | 1.1:-58289012 | MS.gene86822:intron |
| GACTCTTGGGAATCATTGGA+TGG | 0.547060 | 1.1:-58284985 | MS.gene86822:CDS |
| TAGTTGCACCTCATAATGAC+TGG | 0.549693 | 1.1:-58286018 | MS.gene86822:CDS |
| CAGAGGACACTTTAAGAACA+GGG | 0.561148 | 1.1:-58286843 | MS.gene86822:CDS |
| GCGAGGCAAAAGTGATGTAT+TGG | 0.562011 | 1.1:-58284923 | MS.gene86822:CDS |
| GGCAAAAGTGATGTATTGGC+GGG | 0.563534 | 1.1:-58284919 | MS.gene86822:CDS |
| TTCTAAAGATGCTCTGCTTG+AGG | 0.564390 | 1.1:-58290435 | MS.gene86822:CDS |
| ATTGCAACTGTATGGCTGAA+CGG | 0.567248 | 1.1:+58287038 | None:intergenic |
| AGGCAAAAGTGATGTATTGG+CGG | 0.568848 | 1.1:-58284920 | MS.gene86822:CDS |
| GCTCTTCAGCTTCCTTTGTG+CGG | 0.573547 | 1.1:+58289528 | None:intergenic |
| AGCATCTTTAGAAGGTGAAG+TGG | 0.579092 | 1.1:+58290445 | None:intergenic |
| CGTTGTAGGTGTTGATGAGG+AGG | 0.579367 | 1.1:-58292251 | MS.gene86822:CDS |
| GTCTACACTATTTATGTGCG+AGG | 0.583038 | 1.1:-58284940 | MS.gene86822:CDS |
| GGTTGAGCGGAAAACCTGTA+AGG | 0.583881 | 1.1:+58293104 | None:intergenic |
| AGGAGGGACGGTCTAGGCAA+CGG | 0.587494 | 1.1:-58292234 | MS.gene86822:CDS |
| CATCATGTAGTGTGGGACAA+AGG | 0.588356 | 1.1:+58290381 | None:intergenic |
| TCCATGAAGCAATTCTCTCA+TGG | 0.592727 | 1.1:+58293169 | None:intergenic |
| CTTACATACATCATGTAGTG+TGG | 0.595760 | 1.1:+58290373 | None:intergenic |
| AAATATCCAACTTTAAGGTG+TGG | 0.603114 | 1.1:+58286064 | None:intergenic |
| TCAATGCAGAGGCCAGCACC+AGG | 0.604478 | 1.1:+58285679 | None:intergenic |
| CAGCGTTGTAGGTGTTGATG+AGG | 0.612108 | 1.1:-58292254 | MS.gene86822:CDS |
| TGTTTGCATGCAGGAAAGTG+CGG | 0.614003 | 1.1:-58290486 | MS.gene86822:intron |
| TTTAACCGCACCTCCTGCGG+GGG | 0.615781 | 1.1:-58286044 | MS.gene86822:CDS |
| TTCTTACATTTGCTGCCAAG+AGG | 0.617598 | 1.1:-58292210 | MS.gene86822:intron |
| CCAACTTTAAGGTGTGGTAG+CGG | 0.619422 | 1.1:+58286070 | None:intergenic |
| ATATAGCAGTGCCTTGCGGT+TGG | 0.626613 | 1.1:-58286530 | MS.gene86822:CDS |
| TTACATACATCATGTAGTGT+GGG | 0.629806 | 1.1:+58290374 | None:intergenic |
| TAGGTGTTGATGAGGAGGGA+CGG | 0.640085 | 1.1:-58292246 | MS.gene86822:CDS |
| GGTATGCTGCTGCTATTGAG+AGG | 0.640973 | 1.1:-58292015 | MS.gene86822:CDS |
| TGCACTGTACAATTGGGCAT+TGG | 0.643214 | 1.1:-58291975 | MS.gene86822:CDS |
| ATCAGGAGATGCCATGAATG+GGG | 0.644427 | 1.1:-58285773 | MS.gene86822:CDS |
| ATTTAACCGCACCTCCTGCG+GGG | 0.650150 | 1.1:-58286045 | MS.gene86822:CDS |
| GAGGACACTTTAAGAACAGG+GGG | 0.652005 | 1.1:-58286841 | MS.gene86822:CDS |
| CATGAACTGTATCAATGCAG+AGG | 0.654472 | 1.1:+58285668 | None:intergenic |
| TGTACGAACTGCTATCAGCA+AGG | 0.656315 | 1.1:-58287177 | MS.gene86822:intron |
| GTTGTAGGTGTTGATGAGGA+GGG | 0.661388 | 1.1:-58292250 | MS.gene86822:CDS |
| CATAGATCGTACCAACCGCA+AGG | 0.665616 | 1.1:+58286519 | None:intergenic |
| GTTGCAATTTGATTTCCACC+GGG | 0.682764 | 1.1:-58287023 | MS.gene86822:CDS |
| TCTGATCGTGCAAAGATGCG+AGG | 0.685281 | 1.1:-58289551 | MS.gene86822:CDS |
| AAAGATGCGAGGCCGCACAA+AGG | 0.686655 | 1.1:-58289540 | MS.gene86822:CDS |
| TTTACAGTACGGATTAGCAG+AGG | 0.686728 | 1.1:-58286860 | MS.gene86822:intron |
| ATGCAGAGGCCAGCACCAGG+TGG | 0.687236 | 1.1:+58285682 | None:intergenic |
| AGGTATATAGCAGTGCCTTG+CGG | 0.692086 | 1.1:-58286534 | MS.gene86822:CDS |
| TAGTGTGGGACAAAGGCGAG+TGG | 0.693067 | 1.1:+58290388 | None:intergenic |
| CAAGATTGTAAATTGCCCGG+TGG | 0.696463 | 1.1:+58287008 | None:intergenic |
| AGAGGACACTTTAAGAACAG+GGG | 0.707286 | 1.1:-58286842 | MS.gene86822:CDS |
| AGGTACCCAGAGTTGAACAG+AGG | 0.741985 | 1.1:-58285822 | MS.gene86822:CDS |
| AGGACACTTTAAGAACAGGG+GGG | 0.806890 | 1.1:-58286840 | MS.gene86822:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 58284903 | 58293189 | 58284903 | ID=MS.gene86822 |
| chr1.1 | mRNA | 58284903 | 58293189 | 58284903 | ID=MS.gene86822.t1;Parent=MS.gene86822 |
| chr1.1 | exon | 58293119 | 58293189 | 58293119 | ID=MS.gene86822.t1.exon1;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58293119 | 58293189 | 58293119 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| chr1.1 | exon | 58292211 | 58292336 | 58292211 | ID=MS.gene86822.t1.exon2;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58292211 | 58292336 | 58292211 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| chr1.1 | exon | 58291967 | 58292036 | 58291967 | ID=MS.gene86822.t1.exon3;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58291967 | 58292036 | 58291967 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| chr1.1 | exon | 58290382 | 58290495 | 58290382 | ID=MS.gene86822.t1.exon4;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58290382 | 58290495 | 58290382 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| chr1.1 | exon | 58289517 | 58289600 | 58289517 | ID=MS.gene86822.t1.exon5;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58289517 | 58289600 | 58289517 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| chr1.1 | exon | 58289238 | 58289288 | 58289238 | ID=MS.gene86822.t1.exon6;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58289238 | 58289288 | 58289238 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| chr1.1 | exon | 58288999 | 58289028 | 58288999 | ID=MS.gene86822.t1.exon7;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58288999 | 58289028 | 58288999 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| chr1.1 | exon | 58287178 | 58287240 | 58287178 | ID=MS.gene86822.t1.exon8;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58287178 | 58287240 | 58287178 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| chr1.1 | exon | 58286997 | 58287062 | 58286997 | ID=MS.gene86822.t1.exon9;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58286997 | 58287062 | 58286997 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| chr1.1 | exon | 58286747 | 58286875 | 58286747 | ID=MS.gene86822.t1.exon10;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58286747 | 58286875 | 58286747 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| chr1.1 | exon | 58286519 | 58286554 | 58286519 | ID=MS.gene86822.t1.exon11;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58286519 | 58286554 | 58286519 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| chr1.1 | exon | 58285976 | 58286095 | 58285976 | ID=MS.gene86822.t1.exon12;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58285976 | 58286095 | 58285976 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| chr1.1 | exon | 58285654 | 58285842 | 58285654 | ID=MS.gene86822.t1.exon13;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58285654 | 58285842 | 58285654 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| chr1.1 | exon | 58284903 | 58285013 | 58284903 | ID=MS.gene86822.t1.exon14;Parent=MS.gene86822.t1 |
| chr1.1 | CDS | 58284903 | 58285013 | 58284903 | ID=cds.MS.gene86822.t1;Parent=MS.gene86822.t1 |
| Gene Sequence |
| Protein sequence |
