AlfalfaGEDB | MS.gene86921

M. sativa cultivar XinJiangDaYe / MS.gene86921

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86921.t1	XP_013469983.1	93.4	76	5	0	1	76	38	113	3.60E-31	143.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86921.t1	A0A072VQS7	93.4	76	5	0	1	76	38	113	2.6e-31	143.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049573	MS.gene86921	0.821171	4.65E-53	-1.69E-46
MS.gene050363	MS.gene86921	0.803197	3.91E-49	-1.69E-46
MS.gene050883	MS.gene86921	0.809875	1.52E-50	-1.69E-46
MS.gene05142	MS.gene86921	0.843134	1.69E-58	-1.69E-46
MS.gene051946	MS.gene86921	0.848475	5.99E-60	-1.69E-46
MS.gene053826	MS.gene86921	0.805452	1.33E-49	-1.69E-46
MS.gene055401	MS.gene86921	0.850437	1.70E-60	-1.69E-46
MS.gene056310	MS.gene86921	0.861806	7.92E-64	-1.69E-46
MS.gene056311	MS.gene86921	0.838909	2.17E-57	-1.69E-46
MS.gene056313	MS.gene86921	0.847105	1.43E-59	-1.69E-46
MS.gene056408	MS.gene86921	0.809861	1.53E-50	-1.69E-46
MS.gene057014	MS.gene86921	0.830965	2.18E-55	-1.69E-46
MS.gene059236	MS.gene86921	0.804687	1.92E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86921.t1	MTR_1g106215	93.421	76	5	0	1	76	38	113	3.71e-48	148

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGTAGCTACCACATGTTAT+TGG	0.297025	1.2:-79189749	None:intergenic
ATTTGGTAATGATGGAAGTT+TGG	0.326402	1.2:-79189644	MS.gene86921:CDS
TTGGAATCTGACTCATTAAC+AGG	0.333936	1.2:-79189730	MS.gene86921:CDS
GAGCACAGATTTGGTAATGA+TGG	0.406744	1.2:-79189652	MS.gene86921:CDS
AGCATAAGAAGCAGCAGCAC+TGG	0.414803	1.2:+79189576	None:intergenic
AGCTGTCACAATGATGCCTC+TGG	0.415269	1.2:+79189615	None:intergenic
GAGTTCTTAGAGCACAGATT+TGG	0.435020	1.2:-79189661	MS.gene86921:CDS
TGAAATTCTTTGAGGGTGGT+TGG	0.465990	1.2:+79189551	None:intergenic
TGCTCTAAGAACTCAGTTGT+GGG	0.467329	1.2:+79189670	None:intergenic
GAAATTCTTTGAGGGTGGTT+GGG	0.475402	1.2:+79189552	None:intergenic
ATGTTGTTGAAATTCTTTGA+GGG	0.507140	1.2:+79189544	None:intergenic
GCTGTCACAATGATGCCTCT+GGG	0.532008	1.2:+79189616	None:intergenic
GCAGCACTGGCAGTGGTCGG+AGG	0.539102	1.2:+79189589	None:intergenic
GTGCTCTAAGAACTCAGTTG+TGG	0.553458	1.2:+79189669	None:intergenic
GCAGCAGCACTGGCAGTGGT+CGG	0.562924	1.2:+79189586	None:intergenic
AATGTTGTTGAAATTCTTTG+AGG	0.567996	1.2:+79189543	None:intergenic
AGAAGCAGCAGCACTGGCAG+TGG	0.577609	1.2:+79189582	None:intergenic
TTGTTGAAATTCTTTGAGGG+TGG	0.597370	1.2:+79189547	None:intergenic
AGACCTGGTTCTTCTATGAG+AGG	0.610651	1.2:+79189705	None:intergenic
GGAAGTTTGGTTAGCCCCAG+AGG	0.621319	1.2:-79189631	MS.gene86921:CDS
CTGTCACAATGATGCCTCTG+GGG	0.650846	1.2:+79189617	None:intergenic
GATCCTCTCATAGAAGAACC+AGG	0.662887	1.2:-79189708	MS.gene86921:CDS
GGGTTGCTGAAAATCAGACC+TGG	0.666499	1.2:+79189690	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATGTTGTTGAAATTCTTTG+AGG	+	chr1.2:79189724-79189743	None:intergenic	25.0%
!	ATGTTGTTGAAATTCTTTGA+GGG	+	chr1.2:79189723-79189742	None:intergenic	25.0%
!!	ATTTGGTAATGATGGAAGTT+TGG	-	chr1.2:79189620-79189639	MS.gene86921:CDS	30.0%
	TTGGAATCTGACTCATTAAC+AGG	-	chr1.2:79189534-79189553	MS.gene86921:CDS	35.0%
!	TTGTTGAAATTCTTTGAGGG+TGG	+	chr1.2:79189720-79189739	None:intergenic	35.0%
	GAGTTCTTAGAGCACAGATT+TGG	-	chr1.2:79189603-79189622	MS.gene86921:CDS	40.0%
!	GAAATTCTTTGAGGGTGGTT+GGG	+	chr1.2:79189715-79189734	None:intergenic	40.0%
!	TGAAATTCTTTGAGGGTGGT+TGG	+	chr1.2:79189716-79189735	None:intergenic	40.0%
!!	GAGCACAGATTTGGTAATGA+TGG	-	chr1.2:79189612-79189631	MS.gene86921:CDS	40.0%
!!	TGCTCTAAGAACTCAGTTGT+GGG	+	chr1.2:79189597-79189616	None:intergenic	40.0%
	GATCCTCTCATAGAAGAACC+AGG	-	chr1.2:79189556-79189575	MS.gene86921:CDS	45.0%
!	AGACCTGGTTCTTCTATGAG+AGG	+	chr1.2:79189562-79189581	None:intergenic	45.0%
!!	GTGCTCTAAGAACTCAGTTG+TGG	+	chr1.2:79189598-79189617	None:intergenic	45.0%
	AGCATAAGAAGCAGCAGCAC+TGG	+	chr1.2:79189691-79189710	None:intergenic	50.0%
	AGCTGTCACAATGATGCCTC+TGG	+	chr1.2:79189652-79189671	None:intergenic	50.0%
	CTGTCACAATGATGCCTCTG+GGG	+	chr1.2:79189650-79189669	None:intergenic	50.0%
	GCTGTCACAATGATGCCTCT+GGG	+	chr1.2:79189651-79189670	None:intergenic	50.0%
	GGGTTGCTGAAAATCAGACC+TGG	+	chr1.2:79189577-79189596	None:intergenic	50.0%
!	GGAAGTTTGGTTAGCCCCAG+AGG	-	chr1.2:79189633-79189652	MS.gene86921:CDS	55.0%
!!	AGAAGCAGCAGCACTGGCAG+TGG	+	chr1.2:79189685-79189704	None:intergenic	60.0%
!!	GCAGCAGCACTGGCAGTGGT+CGG	+	chr1.2:79189681-79189700	None:intergenic	65.0%
!!	GCAGCACTGGCAGTGGTCGG+AGG	+	chr1.2:79189678-79189697	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	79189528	79189758	79189528	ID=MS.gene86921
chr1.2	mRNA	79189528	79189758	79189528	ID=MS.gene86921.t1;Parent=MS.gene86921
chr1.2	exon	79189528	79189758	79189528	ID=MS.gene86921.t1.exon1;Parent=MS.gene86921.t1
chr1.2	CDS	79189528	79189758	79189528	ID=cds.MS.gene86921.t1;Parent=MS.gene86921.t1

Gene Sequence	>MS.gene86921 ATGTTATTGGAATCTGACTCATTAACAGGATCCTCTCATAGAAGAACCAGGTCTGATTTTCAGCAACCCACAACTGAGTTCTTAGAGCACAGATTTGGTAATGATGGAAGTTTGGTTAGCCCCAGAGGCATCATTGTGACAGCTCTGCCTCCGACCACTGCCAGTGCTGCTGCTTCTTATGCTACCCAACCACCCTCAAAGAATTTCAACAACATTCGCAAACACAAATGA
Protein sequence	>MS.gene86921.t1 MLLESDSLTGSSHRRTRSDFQQPTTEFLEHRFGNDGSLVSPRGIIVTALPPTTASAAASYATQPPSKNFNNIRKHK