Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89225.t1 | XP_003616383.1 | 96 | 126 | 4 | 1 | 1 | 125 | 1 | 126 | 8.80E-51 | 209.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89225.t1 | G7KBN8 | 96.0 | 126 | 4 | 1 | 1 | 125 | 1 | 126 | 6.3e-51 | 209.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050060 | MS.gene89225 | 0.817628 | 2.99E-52 | -1.69E-46 |
| MS.gene050133 | MS.gene89225 | -0.822371 | 2.45E-53 | -1.69E-46 |
| MS.gene050404 | MS.gene89225 | 0.813445 | 2.55E-51 | -1.69E-46 |
| MS.gene050406 | MS.gene89225 | 0.82639 | 2.78E-54 | -1.69E-46 |
| MS.gene050426 | MS.gene89225 | -0.825803 | 3.84E-54 | -1.69E-46 |
| MS.gene051171 | MS.gene89225 | 0.818713 | 1.70E-52 | -1.69E-46 |
| MS.gene051242 | MS.gene89225 | 0.804164 | 2.46E-49 | -1.69E-46 |
| MS.gene052134 | MS.gene89225 | 0.814735 | 1.32E-51 | -1.69E-46 |
| MS.gene052135 | MS.gene89225 | 0.808963 | 2.39E-50 | -1.69E-46 |
| MS.gene052589 | MS.gene89225 | 0.847694 | 9.84E-60 | -1.69E-46 |
| MS.gene052590 | MS.gene89225 | 0.850326 | 1.83E-60 | -1.69E-46 |
| MS.gene053330 | MS.gene89225 | 0.88815 | 7.62E-73 | -1.69E-46 |
| MS.gene054102 | MS.gene89225 | 0.823766 | 1.16E-53 | -1.69E-46 |
| MS.gene054110 | MS.gene89225 | 0.822759 | 1.99E-53 | -1.69E-46 |
| MS.gene055370 | MS.gene89225 | 0.823591 | 1.28E-53 | -1.69E-46 |
| MS.gene05553 | MS.gene89225 | 0.820451 | 6.81E-53 | -1.69E-46 |
| MS.gene056633 | MS.gene89225 | 0.891565 | 3.54E-74 | -1.69E-46 |
| MS.gene056664 | MS.gene89225 | 0.833284 | 5.83E-56 | -1.69E-46 |
| MS.gene058276 | MS.gene89225 | 0.803016 | 4.26E-49 | -1.69E-46 |
| MS.gene058746 | MS.gene89225 | 0.867395 | 1.41E-65 | -1.69E-46 |
| MS.gene059011 | MS.gene89225 | 0.806964 | 6.37E-50 | -1.69E-46 |
| MS.gene059512 | MS.gene89225 | 0.843835 | 1.10E-58 | -1.69E-46 |
| MS.gene061231 | MS.gene89225 | 0.807845 | 4.14E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89225.t1 | MTR_5g079600 | 96.825 | 126 | 3 | 1 | 1 | 125 | 1 | 126 | 1.05e-84 | 243 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTCCAAAGAACTTCTTCT+TGG | 0.271479 | 5.4:-55853596 | None:intergenic |
| GAAACCAAGAAGAAGTTCTT+TGG | 0.299217 | 5.4:+55853592 | MS.gene89225:CDS |
| CGAAATTAGCTTGGTTTCCT+TGG | 0.322975 | 5.4:-55853478 | None:intergenic |
| CAATGGTGAGGATGCTATCT+TGG | 0.372629 | 5.4:+55853535 | MS.gene89225:CDS |
| TTGGAGGAAGAACATCTTGA+TGG | 0.414261 | 5.4:+55853611 | MS.gene89225:CDS |
| AGAACAACAAATAGAATTGT+TGG | 0.440347 | 5.4:-55853354 | None:intergenic |
| GCTCCACACAGAGGCGGAGG+AGG | 0.452211 | 5.4:+55853437 | MS.gene89225:CDS |
| AGCAACGGTGTCTTCGACCA+AGG | 0.493021 | 5.4:+55853461 | MS.gene89225:CDS |
| ACAAATAGAATTGTTGGTGT+TGG | 0.499689 | 5.4:-55853348 | None:intergenic |
| GGGAGAAAAGTGTCGCCCTA+TGG | 0.499821 | 5.4:+55853632 | MS.gene89225:CDS |
| GTTTAGCTTCGAAATTAGCT+TGG | 0.508850 | 5.4:-55853487 | None:intergenic |
| ACCACATAGAAAAGTGATGA+TGG | 0.509265 | 5.4:-55853397 | None:intergenic |
| CCTCACCATTGAAATAGCTC+TGG | 0.520210 | 5.4:-55853523 | None:intergenic |
| AGTACAGGCTGAAGGAGGAT+TGG | 0.522930 | 5.4:+55853563 | MS.gene89225:CDS |
| GAAGCTCCACACAGAGGCGG+AGG | 0.525241 | 5.4:+55853434 | MS.gene89225:CDS |
| TTTAATTTGTTAACATGGCG+AGG | 0.537695 | 5.4:+55853277 | None:intergenic |
| TAGCACCAGAGCTATTTCAA+TGG | 0.550704 | 5.4:+55853518 | MS.gene89225:CDS |
| TGGAGGAAGAACATCTTGAT+GGG | 0.554104 | 5.4:+55853612 | MS.gene89225:CDS |
| ACCAAGAAGAAGTTCTTTGG+AGG | 0.557413 | 5.4:+55853595 | MS.gene89225:CDS |
| GCTATCTTGGAGAAAAGTAC+AGG | 0.569904 | 5.4:+55853548 | MS.gene89225:CDS |
| TAGAATTGTTGGTGTTGGCA+TGG | 0.575328 | 5.4:-55853343 | None:intergenic |
| GCTGAAGGAGGATTGGAAGA+AGG | 0.586228 | 5.4:+55853570 | MS.gene89225:CDS |
| CTTCCTCCTCCGCCTCTGTG+TGG | 0.594778 | 5.4:-55853440 | None:intergenic |
| AAAGTGTCGCCCTATGGACG+AGG | 0.600412 | 5.4:+55853638 | MS.gene89225:CDS |
| TGGAGAAAAGTACAGGCTGA+AGG | 0.610938 | 5.4:+55853555 | MS.gene89225:CDS |
| AGAGGCGGAGGAGGAAGCAA+CGG | 0.613507 | 5.4:+55853446 | MS.gene89225:CDS |
| TGCATGTTAGACACAAGAAG+AGG | 0.637424 | 5.4:-55853300 | None:intergenic |
| CCAGAGCTATTTCAATGGTG+AGG | 0.644609 | 5.4:+55853523 | MS.gene89225:CDS |
| GAAGAAGCTCCACACAGAGG+CGG | 0.672962 | 5.4:+55853431 | MS.gene89225:CDS |
| AGAAAAGTACAGGCTGAAGG+AGG | 0.674459 | 5.4:+55853558 | MS.gene89225:CDS |
| CATGAAGAAGCTCCACACAG+AGG | 0.745545 | 5.4:+55853428 | MS.gene89225:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAACAACAAATAGAATTGT+TGG | - | chr5.4:55853357-55853376 | None:intergenic | 25.0% |
| ACAAATAGAATTGTTGGTGT+TGG | - | chr5.4:55853351-55853370 | None:intergenic | 30.0% | |
| ACCACATAGAAAAGTGATGA+TGG | - | chr5.4:55853400-55853419 | None:intergenic | 35.0% | |
| GAAACCAAGAAGAAGTTCTT+TGG | + | chr5.4:55853592-55853611 | MS.gene89225:CDS | 35.0% | |
| ! | GTTTAGCTTCGAAATTAGCT+TGG | - | chr5.4:55853490-55853509 | None:intergenic | 35.0% |
| !! | TCCATCATCACTTTTCTATG+TGG | + | chr5.4:55853396-55853415 | MS.gene89225:CDS | 35.0% |
| ACCAAGAAGAAGTTCTTTGG+AGG | + | chr5.4:55853595-55853614 | MS.gene89225:CDS | 40.0% | |
| TCCTCCAAAGAACTTCTTCT+TGG | - | chr5.4:55853599-55853618 | None:intergenic | 40.0% | |
| TGCATGTTAGACACAAGAAG+AGG | - | chr5.4:55853303-55853322 | None:intergenic | 40.0% | |
| TGGAGGAAGAACATCTTGAT+GGG | + | chr5.4:55853612-55853631 | MS.gene89225:CDS | 40.0% | |
| TTGGAGGAAGAACATCTTGA+TGG | + | chr5.4:55853611-55853630 | MS.gene89225:CDS | 40.0% | |
| ! | CGAAATTAGCTTGGTTTCCT+TGG | - | chr5.4:55853481-55853500 | None:intergenic | 40.0% |
| ! | GCTATCTTGGAGAAAAGTAC+AGG | + | chr5.4:55853548-55853567 | MS.gene89225:CDS | 40.0% |
| ! | TAGCACCAGAGCTATTTCAA+TGG | + | chr5.4:55853518-55853537 | MS.gene89225:CDS | 40.0% |
| !! | TAGAATTGTTGGTGTTGGCA+TGG | - | chr5.4:55853346-55853365 | None:intergenic | 40.0% |
| CAATGGTGAGGATGCTATCT+TGG | + | chr5.4:55853535-55853554 | MS.gene89225:CDS | 45.0% | |
| CCTCACCATTGAAATAGCTC+TGG | - | chr5.4:55853526-55853545 | None:intergenic | 45.0% | |
| ! | AGAAAAGTACAGGCTGAAGG+AGG | + | chr5.4:55853558-55853577 | MS.gene89225:CDS | 45.0% |
| ! | CCAGAGCTATTTCAATGGTG+AGG | + | chr5.4:55853523-55853542 | MS.gene89225:CDS | 45.0% |
| ! | TGGAGAAAAGTACAGGCTGA+AGG | + | chr5.4:55853555-55853574 | MS.gene89225:CDS | 45.0% |
| AGTACAGGCTGAAGGAGGAT+TGG | + | chr5.4:55853563-55853582 | MS.gene89225:CDS | 50.0% | |
| CATGAAGAAGCTCCACACAG+AGG | + | chr5.4:55853428-55853447 | MS.gene89225:CDS | 50.0% | |
| GCTGAAGGAGGATTGGAAGA+AGG | + | chr5.4:55853570-55853589 | MS.gene89225:CDS | 50.0% | |
| AGCAACGGTGTCTTCGACCA+AGG | + | chr5.4:55853461-55853480 | MS.gene89225:CDS | 55.0% | |
| GAAGAAGCTCCACACAGAGG+CGG | + | chr5.4:55853431-55853450 | MS.gene89225:CDS | 55.0% | |
| !! | AAAGTGTCGCCCTATGGACG+AGG | + | chr5.4:55853638-55853657 | MS.gene89225:CDS | 55.0% |
| !! | GGGAGAAAAGTGTCGCCCTA+TGG | + | chr5.4:55853632-55853651 | MS.gene89225:CDS | 55.0% |
| AGAGGCGGAGGAGGAAGCAA+CGG | + | chr5.4:55853446-55853465 | MS.gene89225:CDS | 60.0% | |
| CTTCCTCCTCCGCCTCTGTG+TGG | - | chr5.4:55853443-55853462 | None:intergenic | 65.0% | |
| GAAGCTCCACACAGAGGCGG+AGG | + | chr5.4:55853434-55853453 | MS.gene89225:CDS | 65.0% | |
| GCTCCACACAGAGGCGGAGG+AGG | + | chr5.4:55853437-55853456 | MS.gene89225:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 55853291 | 55853668 | 55853291 | ID=MS.gene89225 |
| chr5.4 | mRNA | 55853291 | 55853668 | 55853291 | ID=MS.gene89225.t1;Parent=MS.gene89225 |
| chr5.4 | exon | 55853291 | 55853668 | 55853291 | ID=MS.gene89225.t1.exon1;Parent=MS.gene89225.t1 |
| chr5.4 | CDS | 55853291 | 55853668 | 55853291 | ID=cds.MS.gene89225.t1;Parent=MS.gene89225.t1 |
| Gene Sequence |
| Protein sequence |
