Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89594.t1 | PNY09673.1 | 91.4 | 220 | 19 | 0 | 12 | 231 | 88 | 307 | 4.10E-102 | 380.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89594.t1 | Q94A21 | 65.6 | 215 | 68 | 2 | 12 | 226 | 107 | 315 | 9.9e-65 | 248.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89594.t1 | A0A2K3P363 | 91.4 | 220 | 19 | 0 | 12 | 231 | 88 | 307 | 2.9e-102 | 380.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene89594 | 0.8082 | 3.48E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene89594 | 0.821351 | 4.23E-53 | -1.69E-46 |
| MS.gene050941 | MS.gene89594 | 0.802763 | 4.81E-49 | -1.69E-46 |
| MS.gene052108 | MS.gene89594 | 0.81416 | 1.77E-51 | -1.69E-46 |
| MS.gene052954 | MS.gene89594 | 0.830343 | 3.10E-55 | -1.69E-46 |
| MS.gene053006 | MS.gene89594 | 0.800933 | 1.14E-48 | -1.69E-46 |
| MS.gene053963 | MS.gene89594 | 0.818624 | 1.78E-52 | -1.69E-46 |
| MS.gene054479 | MS.gene89594 | 0.807882 | 4.07E-50 | -1.69E-46 |
| MS.gene05517 | MS.gene89594 | 0.843959 | 1.02E-58 | -1.69E-46 |
| MS.gene055623 | MS.gene89594 | 0.802492 | 5.47E-49 | -1.69E-46 |
| MS.gene055841 | MS.gene89594 | 0.819239 | 1.29E-52 | -1.69E-46 |
| MS.gene056776 | MS.gene89594 | 0.814633 | 1.40E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene89594 | 0.814965 | 1.18E-51 | -1.69E-46 |
| MS.gene058008 | MS.gene89594 | 0.813441 | 2.56E-51 | -1.69E-46 |
| MS.gene058009 | MS.gene89594 | 0.810226 | 1.28E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene89594 | 0.831905 | 1.28E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene89594 | 0.825443 | 4.68E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89594.t1 | MTR_8g077020 | 97.273 | 220 | 6 | 0 | 12 | 231 | 88 | 307 | 6.30e-156 | 434 |
| MS.gene89594.t1 | MTR_4g114550 | 44.560 | 193 | 94 | 4 | 11 | 197 | 83 | 268 | 4.03e-42 | 144 |
| MS.gene89594.t1 | MTR_4g027390 | 42.077 | 183 | 87 | 2 | 12 | 194 | 78 | 241 | 3.16e-37 | 131 |
| MS.gene89594.t1 | MTR_1g012440 | 37.629 | 194 | 107 | 3 | 11 | 196 | 81 | 268 | 4.05e-32 | 118 |
| MS.gene89594.t1 | MTR_3g107393 | 38.919 | 185 | 87 | 5 | 11 | 185 | 68 | 236 | 3.83e-29 | 110 |
| MS.gene89594.t1 | MTR_0262s0060 | 41.905 | 105 | 54 | 1 | 12 | 116 | 67 | 164 | 4.13e-20 | 85.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89594.t1 | AT5G15330 | 65.581 | 215 | 68 | 2 | 12 | 226 | 107 | 315 | 2.70e-89 | 266 |
| MS.gene89594.t1 | AT5G15330 | 65.581 | 215 | 68 | 2 | 12 | 226 | 176 | 384 | 5.50e-88 | 265 |
| MS.gene89594.t1 | AT5G20150 | 40.642 | 187 | 81 | 4 | 11 | 194 | 77 | 236 | 1.78e-36 | 129 |
| MS.gene89594.t1 | AT2G26660 | 39.409 | 203 | 101 | 5 | 11 | 203 | 84 | 274 | 4.55e-36 | 129 |
| MS.gene89594.t1 | AT2G45130 | 39.344 | 183 | 89 | 4 | 12 | 194 | 64 | 224 | 3.70e-33 | 120 |
Find 42 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGTACCTGCAAAGTTTA+AGG | 0.146732 | 8.3:+27113203 | None:intergenic |
| TACAGTTCCTTAAACTTTGC+AGG | 0.292364 | 8.3:-27113210 | MS.gene89594:intron |
| AAAAGAGAAGAAAATGAAAA+TGG | 0.299936 | 8.3:+27112451 | None:intergenic |
| CGAAGAACGATTTGTGTAAA+AGG | 0.308457 | 8.3:+27112739 | None:intergenic |
| TTAGTCATTGTCCTCTTTAC+CGG | 0.330019 | 8.3:+27112339 | None:intergenic |
| AGAAAATGAAAATGGATTGC+AGG | 0.353750 | 8.3:+27112459 | None:intergenic |
| TGCTGCAATGAGAGCCATAA+AGG | 0.361294 | 8.3:-27112503 | MS.gene89594:CDS |
| GGTGGTCTAGACTCGTGTTC+TGG | 0.366418 | 8.3:+27112607 | None:intergenic |
| GTGAAGAAATGATGGACATC+AGG | 0.455962 | 8.3:-27113278 | MS.gene89594:CDS |
| CCAAGAGTTGACGATGTCTC+TGG | 0.455990 | 8.3:+27112559 | None:intergenic |
| TATAATGTTGTTTGTTGACT+TGG | 0.466657 | 8.3:-27113382 | MS.gene89594:CDS |
| TGAATTTAGTGAAGAAATGA+TGG | 0.467079 | 8.3:-27113286 | MS.gene89594:CDS |
| CCAGAGACATCGTCAACTCT+TGG | 0.470324 | 8.3:-27112559 | MS.gene89594:CDS |
| CTAGACTCGTGTTCTGGATG+AGG | 0.479440 | 8.3:+27112613 | None:intergenic |
| GTTGTTTGTTGACTTGGAAC+AGG | 0.479560 | 8.3:-27113376 | MS.gene89594:CDS |
| AAGAAGTATGATAAAAGAAC+CGG | 0.483802 | 8.3:-27112778 | MS.gene89594:CDS |
| TCCAGATACCTTGCAAAATG+AGG | 0.505465 | 8.3:-27112368 | MS.gene89594:CDS |
| GCTGCAATGAGAGCCATAAA+GGG | 0.508490 | 8.3:-27112502 | MS.gene89594:CDS |
| AAGGACTTGGTTACCATCCA+TGG | 0.518555 | 8.3:-27113255 | MS.gene89594:CDS |
| TTTAGCAACCAAGACGAAGA+TGG | 0.520909 | 8.3:-27112430 | MS.gene89594:CDS |
| TGAGGAAGACACCGGTAAAG+AGG | 0.528351 | 8.3:-27112350 | MS.gene89594:CDS |
| TGAATTACTTCTTCTTCTAG+AGG | 0.528730 | 8.3:+27112643 | None:intergenic |
| GATGGACATCAGGAAGGACT+TGG | 0.539190 | 8.3:-27113268 | MS.gene89594:CDS |
| CACCAGTACCATCTTCGTCT+TGG | 0.542694 | 8.3:+27112422 | None:intergenic |
| GTATTCGTTGTGCTATCTAC+CGG | 0.564756 | 8.3:+27112586 | None:intergenic |
| AACCAAGACGAAGATGGTAC+TGG | 0.568449 | 8.3:-27112424 | MS.gene89594:CDS |
| TTAGGAGCACCATCTCTCCA+TGG | 0.586091 | 8.3:+27113238 | None:intergenic |
| TCACTACTGAGCCTCTGACT+AGG | 0.595158 | 8.3:-27112705 | MS.gene89594:CDS |
| GGTTACCATCCATGGAGAGA+TGG | 0.599706 | 8.3:-27113247 | MS.gene89594:CDS |
| GAGCACCATCTCTCCATGGA+TGG | 0.602744 | 8.3:+27113242 | None:intergenic |
| AAATGAAAATGGATTGCAGG+TGG | 0.623783 | 8.3:+27112462 | None:intergenic |
| TTCGTTGTGCTATCTACCGG+TGG | 0.626489 | 8.3:+27112589 | None:intergenic |
| AGAAATGATGGACATCAGGA+AGG | 0.632825 | 8.3:-27113274 | MS.gene89594:CDS |
| AAGTATGATAAAAGAACCGG+CGG | 0.635105 | 8.3:-27112775 | MS.gene89594:CDS |
| TAGGCTAGTTCATGAATGTG+AGG | 0.641042 | 8.3:-27112686 | MS.gene89594:CDS |
| GACATCGTCAACTCTTGGAG+AGG | 0.650383 | 8.3:-27112554 | MS.gene89594:CDS |
| AGAACACGAGTCTAGACCAC+CGG | 0.650727 | 8.3:-27112605 | MS.gene89594:CDS |
| AGGTAGTTGCAACAACCCGC+CGG | 0.651351 | 8.3:+27112759 | None:intergenic |
| TTGCAAAATGAGGAAGACAC+CGG | 0.699666 | 8.3:-27112358 | MS.gene89594:CDS |
| TCATGAACTAGCCTAGTCAG+AGG | 0.707862 | 8.3:+27112694 | None:intergenic |
| AGTATGATAAAAGAACCGGC+GGG | 0.751372 | 8.3:-27112774 | MS.gene89594:CDS |
| TGTAACTGCTGAAAACTCTG+CGG | 0.777311 | 8.3:-27112398 | MS.gene89594:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAGAAGAAAATGAAAA+TGG | + | chr8.3:27113296-27113315 | None:intergenic | 20.0% |
| !! | ATCTGTATGACAAAATATTA+AGG | + | chr8.3:27112824-27112843 | None:intergenic | 20.0% |
| ! | TGAATTTAGTGAAGAAATGA+TGG | - | chr8.3:27112458-27112477 | MS.gene89594:CDS | 25.0% |
| !! | AAGAAGTATGATAAAAGAAC+CGG | - | chr8.3:27112966-27112985 | MS.gene89594:intron | 25.0% |
| !! | ATGACACTTTTACATTAAAG+TGG | + | chr8.3:27112789-27112808 | None:intergenic | 25.0% |
| !! | TATAATGTTGTTTGTTGACT+TGG | - | chr8.3:27112362-27112381 | MS.gene89594:CDS | 25.0% |
| !!! | GTAAAAGTGTCATTTTTGTA+AGG | - | chr8.3:27112796-27112815 | MS.gene89594:CDS | 25.0% |
| !!! | TTAAGGAACTGTAGTTTTTT+AGG | + | chr8.3:27112527-27112546 | None:intergenic | 25.0% |
| GTATCTACACTTCATATTGA+AGG | - | chr8.3:27112607-27112626 | MS.gene89594:CDS | 30.0% | |
| TAACATATTTCCACTACAAC+AGG | - | chr8.3:27112927-27112946 | MS.gene89594:intron | 30.0% | |
| TGAATTACTTCTTCTTCTAG+AGG | + | chr8.3:27113104-27113123 | None:intergenic | 30.0% | |
| TTTAATTAGTCCTGTTGTAG+TGG | + | chr8.3:27112940-27112959 | None:intergenic | 30.0% | |
| !! | AGAAAATGAAAATGGATTGC+AGG | + | chr8.3:27113288-27113307 | None:intergenic | 30.0% |
| AAGTATGATAAAAGAACCGG+CGG | - | chr8.3:27112969-27112988 | MS.gene89594:intron | 35.0% | |
| CGAAGAACGATTTGTGTAAA+AGG | + | chr8.3:27113008-27113027 | None:intergenic | 35.0% | |
| TACAGTTCCTTAAACTTTGC+AGG | - | chr8.3:27112534-27112553 | MS.gene89594:CDS | 35.0% | |
| TATGTTGCATATGTGTGATG+AGG | - | chr8.3:27112871-27112890 | MS.gene89594:intron | 35.0% | |
| TGTTGTACCTGCAAAGTTTA+AGG | + | chr8.3:27112544-27112563 | None:intergenic | 35.0% | |
| TTAGTCATTGTCCTCTTTAC+CGG | + | chr8.3:27113408-27113427 | None:intergenic | 35.0% | |
| ! | ACACTTCATATTGAAGGTGT+AGG | - | chr8.3:27112613-27112632 | MS.gene89594:CDS | 35.0% |
| !! | AAATGAAAATGGATTGCAGG+TGG | + | chr8.3:27113285-27113304 | None:intergenic | 35.0% |
| !! | CGCTTTTTGTAAACCCTTTA+TGG | + | chr8.3:27113258-27113277 | None:intergenic | 35.0% |
| !! | TGCTTTAGAGTATGACTATG+TGG | - | chr8.3:27112757-27112776 | MS.gene89594:CDS | 35.0% |
| ACATGTAACAGTGTTCGACA+TGG | + | chr8.3:27112666-27112685 | None:intergenic | 40.0% | |
| AGAGGCTCAGTAGTGAAAAA+AGG | + | chr8.3:27113035-27113054 | None:intergenic | 40.0% | |
| AGTATGATAAAAGAACCGGC+GGG | - | chr8.3:27112970-27112989 | MS.gene89594:intron | 40.0% | |
| ATGTCGAACACTGTTACATG+TGG | - | chr8.3:27112665-27112684 | MS.gene89594:CDS | 40.0% | |
| GTATTCGTTGTGCTATCTAC+CGG | + | chr8.3:27113161-27113180 | None:intergenic | 40.0% | |
| TCCAGATACCTTGCAAAATG+AGG | - | chr8.3:27113376-27113395 | MS.gene89594:CDS | 40.0% | |
| TGTAACTGCTGAAAACTCTG+CGG | - | chr8.3:27113346-27113365 | MS.gene89594:CDS | 40.0% | |
| TTGCAAAATGAGGAAGACAC+CGG | - | chr8.3:27113386-27113405 | MS.gene89594:CDS | 40.0% | |
| TTTAGCAACCAAGACGAAGA+TGG | - | chr8.3:27113314-27113333 | MS.gene89594:CDS | 40.0% | |
| ! | TAGGCTAGTTCATGAATGTG+AGG | - | chr8.3:27113058-27113077 | MS.gene89594:intron | 40.0% |
| !! | AGAAATGATGGACATCAGGA+AGG | - | chr8.3:27112470-27112489 | MS.gene89594:CDS | 40.0% |
| !! | CAGAAGTGTACTTTTCACTC+TGG | + | chr8.3:27112434-27112453 | None:intergenic | 40.0% |
| !! | GTGAAGAAATGATGGACATC+AGG | - | chr8.3:27112466-27112485 | MS.gene89594:CDS | 40.0% |
| !! | GTTGTTTGTTGACTTGGAAC+AGG | - | chr8.3:27112368-27112387 | MS.gene89594:CDS | 40.0% |
| !! | TCCTCATTTTGCAAGGTATC+TGG | + | chr8.3:27113380-27113399 | None:intergenic | 40.0% |
| AACCAAGACGAAGATGGTAC+TGG | - | chr8.3:27113320-27113339 | MS.gene89594:CDS | 45.0% | |
| GCTGCAATGAGAGCCATAAA+GGG | - | chr8.3:27113242-27113261 | MS.gene89594:CDS | 45.0% | |
| TAGGCAGTGTCTAACATGTG+TGG | - | chr8.3:27112632-27112651 | MS.gene89594:CDS | 45.0% | |
| TGCTGCAATGAGAGCCATAA+AGG | - | chr8.3:27113241-27113260 | MS.gene89594:CDS | 45.0% | |
| ! | AAGGACTTGGTTACCATCCA+TGG | - | chr8.3:27112489-27112508 | MS.gene89594:CDS | 45.0% |
| ! | GGTGTCTTCCTCATTTTGCA+AGG | + | chr8.3:27113387-27113406 | None:intergenic | 45.0% |
| ! | TCATGAACTAGCCTAGTCAG+AGG | + | chr8.3:27113053-27113072 | None:intergenic | 45.0% |
| AGAACACGAGTCTAGACCAC+CGG | - | chr8.3:27113139-27113158 | MS.gene89594:intron | 50.0% | |
| CACCAGTACCATCTTCGTCT+TGG | + | chr8.3:27113325-27113344 | None:intergenic | 50.0% | |
| CCAAGAGTTGACGATGTCTC+TGG | + | chr8.3:27113188-27113207 | None:intergenic | 50.0% | |
| CCAGAGACATCGTCAACTCT+TGG | - | chr8.3:27113185-27113204 | MS.gene89594:intron | 50.0% | |
| GACATCGTCAACTCTTGGAG+AGG | - | chr8.3:27113190-27113209 | MS.gene89594:intron | 50.0% | |
| GATGGACATCAGGAAGGACT+TGG | - | chr8.3:27112476-27112495 | MS.gene89594:CDS | 50.0% | |
| GGTTACCATCCATGGAGAGA+TGG | - | chr8.3:27112497-27112516 | MS.gene89594:CDS | 50.0% | |
| GTGTCTAACATGTGTGGTGC+TGG | - | chr8.3:27112638-27112657 | MS.gene89594:CDS | 50.0% | |
| TCACTACTGAGCCTCTGACT+AGG | - | chr8.3:27113039-27113058 | MS.gene89594:intron | 50.0% | |
| TGAGGAAGACACCGGTAAAG+AGG | - | chr8.3:27113394-27113413 | MS.gene89594:CDS | 50.0% | |
| TTCGTTGTGCTATCTACCGG+TGG | + | chr8.3:27113158-27113177 | None:intergenic | 50.0% | |
| ! | CTAGACTCGTGTTCTGGATG+AGG | + | chr8.3:27113134-27113153 | None:intergenic | 50.0% |
| !! | TTAGGAGCACCATCTCTCCA+TGG | + | chr8.3:27112509-27112528 | None:intergenic | 50.0% |
| AGGTAGTTGCAACAACCCGC+CGG | + | chr8.3:27112988-27113007 | None:intergenic | 55.0% | |
| GAGCACCATCTCTCCATGGA+TGG | + | chr8.3:27112505-27112524 | None:intergenic | 55.0% | |
| GGTGGTCTAGACTCGTGTTC+TGG | + | chr8.3:27113140-27113159 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 27112339 | 27113427 | 27112339 | ID=MS.gene89594 |
| chr8.3 | mRNA | 27112339 | 27113427 | 27112339 | ID=MS.gene89594.t1;Parent=MS.gene89594 |
| chr8.3 | exon | 27113211 | 27113427 | 27113211 | ID=MS.gene89594.t1.exon1;Parent=MS.gene89594.t1 |
| chr8.3 | CDS | 27113211 | 27113427 | 27113211 | ID=cds.MS.gene89594.t1;Parent=MS.gene89594.t1 |
| chr8.3 | exon | 27112339 | 27112817 | 27112339 | ID=MS.gene89594.t1.exon2;Parent=MS.gene89594.t1 |
| chr8.3 | CDS | 27112339 | 27112817 | 27112339 | ID=cds.MS.gene89594.t1;Parent=MS.gene89594.t1 |
| Gene Sequence |
| Protein sequence |
