Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90467.t1 | KEH31485.1 | 88.3 | 537 | 60 | 2 | 1 | 535 | 1 | 536 | 8.90E-201 | 709.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90467.t1 | Q9FE83 | 42.7 | 492 | 269 | 4 | 39 | 527 | 41 | 522 | 9.5e-103 | 375.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90467.t1 | A0A072V054 | 88.3 | 537 | 60 | 2 | 1 | 535 | 1 | 536 | 6.4e-201 | 709.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene90467 | 0.802439 | 5.61E-49 | -1.69E-46 |
| MS.gene04998 | MS.gene90467 | 0.854449 | 1.22E-61 | -1.69E-46 |
| MS.gene050414 | MS.gene90467 | 0.837341 | 5.50E-57 | -1.69E-46 |
| MS.gene051043 | MS.gene90467 | 0.801825 | 7.50E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene90467 | 0.844551 | 7.05E-59 | -1.69E-46 |
| MS.gene051819 | MS.gene90467 | 0.816043 | 6.78E-52 | -1.69E-46 |
| MS.gene052016 | MS.gene90467 | 0.817501 | 3.19E-52 | -1.69E-46 |
| MS.gene054178 | MS.gene90467 | 0.810356 | 1.20E-50 | -1.69E-46 |
| MS.gene054617 | MS.gene90467 | 0.809672 | 1.69E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene90467 | 0.802925 | 4.45E-49 | -1.69E-46 |
| MS.gene054893 | MS.gene90467 | 0.80744 | 5.05E-50 | -1.69E-46 |
| MS.gene057093 | MS.gene90467 | 0.834623 | 2.69E-56 | -1.69E-46 |
| MS.gene060563 | MS.gene90467 | 0.809336 | 1.99E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene90467 | 0.805865 | 1.09E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90467.t1 | MTR_4g094932 | 96.462 | 537 | 16 | 2 | 1 | 535 | 1 | 536 | 0.0 | 1038 |
| MS.gene90467.t1 | MTR_1g018790 | 52.381 | 525 | 230 | 5 | 6 | 523 | 27 | 538 | 0.0 | 520 |
| MS.gene90467.t1 | MTR_2g021030 | 28.043 | 460 | 253 | 12 | 44 | 432 | 11 | 463 | 1.78e-26 | 114 |
| MS.gene90467.t1 | MTR_2g021030 | 27.434 | 339 | 192 | 11 | 113 | 426 | 199 | 508 | 2.92e-17 | 85.5 |
| MS.gene90467.t1 | MTR_2g021030 | 25.140 | 358 | 247 | 11 | 78 | 428 | 245 | 588 | 1.44e-16 | 83.2 |
| MS.gene90467.t1 | MTR_2g021030 | 24.891 | 458 | 244 | 11 | 44 | 432 | 11 | 437 | 6.13e-22 | 99.0 |
| MS.gene90467.t1 | MTR_4g013255 | 28.231 | 294 | 185 | 10 | 114 | 398 | 342 | 618 | 6.25e-16 | 81.3 |
| MS.gene90467.t1 | MTR_4g013255 | 27.610 | 431 | 239 | 13 | 39 | 436 | 12 | 402 | 3.22e-14 | 75.9 |
| MS.gene90467.t1 | MTR_4g013255 | 26.220 | 328 | 199 | 9 | 130 | 426 | 256 | 571 | 3.73e-14 | 75.9 |
| MS.gene90467.t1 | MTR_5g088767 | 61.765 | 68 | 24 | 1 | 200 | 267 | 27 | 92 | 8.51e-16 | 73.6 |
| MS.gene90467.t1 | MTR_1g114610 | 26.442 | 416 | 259 | 14 | 30 | 433 | 6 | 386 | 3.17e-14 | 75.1 |
| MS.gene90467.t1 | MTR_1g012520 | 24.665 | 373 | 215 | 15 | 114 | 456 | 224 | 560 | 1.12e-13 | 74.3 |
| MS.gene90467.t1 | MTR_1g012520 | 24.070 | 457 | 281 | 17 | 42 | 448 | 66 | 506 | 2.47e-12 | 70.1 |
| MS.gene90467.t1 | MTR_1g012520 | 31.390 | 223 | 115 | 10 | 267 | 457 | 191 | 407 | 1.88e-11 | 67.0 |
| MS.gene90467.t1 | MTR_7g083610 | 25.837 | 418 | 261 | 20 | 38 | 426 | 1 | 398 | 3.17e-13 | 72.8 |
| MS.gene90467.t1 | MTR_7g112570 | 25.414 | 362 | 211 | 11 | 114 | 449 | 193 | 521 | 4.56e-13 | 72.4 |
| MS.gene90467.t1 | MTR_7g112570 | 27.500 | 320 | 216 | 11 | 118 | 432 | 273 | 581 | 9.51e-12 | 68.2 |
| MS.gene90467.t1 | MTR_7g112570 | 25.414 | 362 | 211 | 11 | 114 | 449 | 193 | 521 | 5.64e-13 | 72.0 |
| MS.gene90467.t1 | MTR_7g112570 | 27.500 | 320 | 216 | 11 | 118 | 432 | 273 | 581 | 1.27e-11 | 67.8 |
| MS.gene90467.t1 | MTR_7g112570 | 25.414 | 362 | 211 | 11 | 114 | 449 | 193 | 521 | 6.96e-13 | 71.6 |
| MS.gene90467.t1 | MTR_7g112570 | 27.500 | 320 | 216 | 11 | 118 | 432 | 273 | 581 | 1.52e-11 | 67.4 |
| MS.gene90467.t1 | MTR_4g091290 | 21.600 | 500 | 273 | 18 | 36 | 427 | 18 | 506 | 1.41e-11 | 67.4 |
| MS.gene90467.t1 | MTR_3g107280 | 30.058 | 173 | 113 | 4 | 317 | 481 | 160 | 332 | 4.98e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90467.t1 | AT5G67250 | 53.889 | 540 | 220 | 6 | 1 | 528 | 1 | 523 | 0.0 | 538 |
| MS.gene90467.t1 | AT1G47056 | 51.633 | 490 | 224 | 4 | 35 | 521 | 34 | 513 | 1.19e-174 | 503 |
| MS.gene90467.t1 | AT3G50080 | 49.338 | 529 | 257 | 4 | 1 | 528 | 1 | 519 | 9.73e-169 | 488 |
| MS.gene90467.t1 | AT4G07400 | 48.084 | 522 | 249 | 4 | 1 | 518 | 38 | 541 | 1.02e-165 | 481 |
| MS.gene90467.t1 | AT4G15475 | 26.087 | 460 | 270 | 10 | 44 | 436 | 11 | 467 | 3.46e-26 | 113 |
| MS.gene90467.t1 | AT4G15475 | 24.219 | 384 | 246 | 12 | 77 | 428 | 218 | 588 | 1.96e-16 | 82.8 |
| MS.gene90467.t1 | AT4G15475 | 24.024 | 333 | 209 | 8 | 107 | 408 | 274 | 593 | 1.31e-14 | 77.0 |
| MS.gene90467.t1 | AT2G25490 | 24.625 | 467 | 258 | 17 | 44 | 451 | 67 | 498 | 1.57e-15 | 80.1 |
| MS.gene90467.t1 | AT2G25490 | 28.011 | 357 | 211 | 15 | 109 | 429 | 208 | 554 | 1.21e-14 | 77.4 |
| MS.gene90467.t1 | AT3G26810 | 23.611 | 432 | 254 | 16 | 38 | 426 | 1 | 399 | 1.54e-13 | 73.6 |
| MS.gene90467.t1 | AT5G23340 | 25.243 | 412 | 258 | 16 | 35 | 433 | 1 | 375 | 4.32e-13 | 71.6 |
| MS.gene90467.t1 | AT5G27920 | 28.912 | 294 | 123 | 9 | 138 | 429 | 332 | 541 | 4.48e-13 | 72.4 |
| MS.gene90467.t1 | AT5G27920 | 25.208 | 361 | 224 | 9 | 114 | 441 | 155 | 502 | 4.60e-13 | 72.4 |
| MS.gene90467.t1 | AT5G01720 | 28.689 | 244 | 149 | 8 | 114 | 348 | 338 | 565 | 1.16e-12 | 71.2 |
| MS.gene90467.t1 | AT1G21410 | 32.911 | 158 | 96 | 6 | 285 | 436 | 86 | 239 | 5.16e-12 | 67.8 |
| MS.gene90467.t1 | AT1G21410 | 32.911 | 158 | 96 | 6 | 285 | 436 | 86 | 239 | 5.16e-12 | 67.8 |
| MS.gene90467.t1 | AT5G25350 | 30.882 | 204 | 106 | 7 | 277 | 449 | 180 | 379 | 1.05e-11 | 67.8 |
| MS.gene90467.t1 | AT1G77000 | 31.818 | 154 | 99 | 5 | 287 | 436 | 88 | 239 | 1.24e-11 | 65.5 |
| MS.gene90467.t1 | AT1G77000 | 31.818 | 154 | 99 | 5 | 287 | 436 | 88 | 239 | 1.24e-11 | 65.5 |
| MS.gene90467.t1 | AT1G77000 | 31.818 | 154 | 99 | 5 | 287 | 436 | 88 | 239 | 4.01e-11 | 65.1 |
| MS.gene90467.t1 | AT1G77000 | 31.818 | 154 | 99 | 5 | 287 | 436 | 88 | 239 | 4.01e-11 | 65.1 |
| MS.gene90467.t1 | AT5G57900 | 30.270 | 185 | 112 | 6 | 254 | 434 | 77 | 248 | 5.46e-11 | 64.3 |
Find 128 sgRNAs with CRISPR-Local
Find 139 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTCTGGGTGTCCTAATT+TGG | 0.160295 | 8.3:+23234585 | MS.gene90467:CDS |
| ACTTAAGAAATTGTGCATTA+AGG | 0.210448 | 8.3:+23234528 | MS.gene90467:CDS |
| TCCTTTGCTCCGCTGATAAT+TGG | 0.220301 | 8.3:+23234031 | MS.gene90467:CDS |
| GATTTCAAAGTGTTTGAAAT+TGG | 0.228739 | 8.3:+23234195 | MS.gene90467:CDS |
| ACTAAACGCGGAGGCGAAAT+TGG | 0.243805 | 8.3:+23233592 | MS.gene90467:CDS |
| GTTACTGAGATTGGGATGTT+AGG | 0.255411 | 8.3:+23233773 | MS.gene90467:CDS |
| CTTAAGAAATTGTGCATTAA+GGG | 0.276369 | 8.3:+23234529 | MS.gene90467:CDS |
| ACCTTGAGTTTAACCAAATT+AGG | 0.303407 | 8.3:-23234598 | None:intergenic |
| TTGTTATTGCTGAGCGTTGC+AGG | 0.308812 | 8.3:+23234266 | MS.gene90467:CDS |
| TTCCGTTGCCGTAACGGAAT+CGG | 0.315769 | 8.3:-23233394 | None:intergenic |
| GTATCCTACGTCGTTGAGTT+TGG | 0.321368 | 8.3:+23234402 | MS.gene90467:CDS |
| TTGGAAAGGTTTGCTCTTTG+TGG | 0.330772 | 8.3:+23234451 | MS.gene90467:CDS |
| ACTGTGGGGAAGATGAATTC+TGG | 0.344822 | 8.3:+23234118 | MS.gene90467:CDS |
| GCGGTTAAAGAGGAGTTCTT+TGG | 0.346706 | 8.3:+23234663 | MS.gene90467:CDS |
| CAGAATTGGTGATGAGGGTT+TGG | 0.348847 | 8.3:+23234327 | MS.gene90467:CDS |
| TGGTGATGAGGGTTTGGTTT+CGG | 0.355191 | 8.3:+23234333 | MS.gene90467:CDS |
| TCGTGTTTGTTTGGTGTTAA+GGG | 0.357584 | 8.3:+23233839 | MS.gene90467:CDS |
| GTTGAGATCGATTTGTTTGA+AGG | 0.361908 | 8.3:+23233991 | MS.gene90467:CDS |
| TCGATGTTCAGGACCAAGTT+TGG | 0.368185 | 8.3:+23234841 | MS.gene90467:CDS |
| GTTGAATCGGTTTGTGGTAA+TGG | 0.374184 | 8.3:+23233959 | MS.gene90467:CDS |
| TAAGAATTTGAAGAAGCTTT+CGG | 0.379689 | 8.3:+23233811 | MS.gene90467:CDS |
| TTCGTGTTTGTTTGGTGTTA+AGG | 0.380448 | 8.3:+23233838 | MS.gene90467:CDS |
| TGGAAAGGTTTGCTCTTTGT+GGG | 0.380935 | 8.3:+23234452 | MS.gene90467:CDS |
| TTCAAACTTTGAAGCTGATT+CGG | 0.382144 | 8.3:+23234065 | MS.gene90467:CDS |
| AACACAAGCTCTTGCAAGTT+AGG | 0.382811 | 8.3:-23234370 | None:intergenic |
| TATCCTACGTCGTTGAGTTT+GGG | 0.388016 | 8.3:+23234403 | MS.gene90467:CDS |
| TGCTTCGTGTTGTCTGAGTT+TGG | 0.393428 | 8.3:+23234432 | MS.gene90467:CDS |
| GTTAGTGATGTGGGTCTTGT+TGG | 0.395867 | 8.3:+23234172 | MS.gene90467:CDS |
| TCGGTTGTTTCGTGTTTGTT+TGG | 0.400969 | 8.3:+23233830 | MS.gene90467:CDS |
| TCCGTTGCCGTAACGGAATC+GGG | 0.402578 | 8.3:-23233393 | None:intergenic |
| TGTAGGGAAGTTACTGAGAT+TGG | 0.403010 | 8.3:+23233764 | MS.gene90467:CDS |
| GGTGATTGGGATTCGATGCT+TGG | 0.409148 | 8.3:+23234094 | MS.gene90467:CDS |
| TTACTGAGATTGGGATGTTA+GGG | 0.411147 | 8.3:+23233774 | MS.gene90467:CDS |
| AGGTTTGCTCTTTGTGGGAT+TGG | 0.411999 | 8.3:+23234457 | MS.gene90467:CDS |
| TTGAATCGGTTTGTGGTAAT+GGG | 0.413048 | 8.3:+23233960 | MS.gene90467:CDS |
| AGAAAGTGTGTGAAAGTTAC+TGG | 0.421944 | 8.3:+23234622 | MS.gene90467:CDS |
| GTCGAGGAAGTGAAGTATTC+CGG | 0.426679 | 8.3:-23233492 | None:intergenic |
| TTGAGGAACTTTCTGTTAAG+AGG | 0.443817 | 8.3:+23233894 | MS.gene90467:CDS |
| ATAACCGATTGTCGATGTTC+AGG | 0.443985 | 8.3:+23234830 | MS.gene90467:CDS |
| TGATTCGGTGTATTGGTGAT+TGG | 0.449636 | 8.3:+23234080 | MS.gene90467:CDS |
| GATTCGGTGTATTGGTGATT+GGG | 0.453256 | 8.3:+23234081 | MS.gene90467:CDS |
| GTGATTGGGATTCGATGCTT+GGG | 0.455550 | 8.3:+23234095 | MS.gene90467:CDS |
| TAACGGAATCGGGTTACATC+TGG | 0.459880 | 8.3:-23233383 | None:intergenic |
| TTGAAGCTGATTCGGTGTAT+TGG | 0.461688 | 8.3:+23234073 | MS.gene90467:CDS |
| TTCCGTTACGGCAACGGAAA+CGG | 0.461833 | 8.3:+23233398 | MS.gene90467:CDS |
| TACCGTTTCCGTTGCCGTAA+CGG | 0.461895 | 8.3:-23233400 | None:intergenic |
| CTTGAAGCTTCGCGCGTGTA+GGG | 0.462348 | 8.3:+23233748 | MS.gene90467:CDS |
| GTGAAGCAGCGGGGTGGCTG+CGG | 0.464487 | 8.3:+23234644 | MS.gene90467:CDS |
| CTGTGGGGAAGATGAATTCT+GGG | 0.467634 | 8.3:+23234119 | MS.gene90467:CDS |
| TTAGTGATGTGGGTCTTGTT+GGG | 0.468421 | 8.3:+23234173 | MS.gene90467:CDS |
| CGAAGGAGAATCATCGATAA+GGG | 0.469027 | 8.3:-23234791 | None:intergenic |
| CGATGTTAACGAGGTTAATG+AGG | 0.469138 | 8.3:+23233931 | MS.gene90467:CDS |
| ACCCGATTCCGTTACGGCAA+CGG | 0.472699 | 8.3:+23233392 | MS.gene90467:CDS |
| TGCCGTGTGCGTTCAGAAGG+TGG | 0.472963 | 8.3:+23234887 | MS.gene90467:CDS |
| TGTTTGAAGGAGCTTGTTAA+CGG | 0.474705 | 8.3:+23234004 | MS.gene90467:CDS |
| GGATTCGATGCTTGGGACTG+TGG | 0.479553 | 8.3:+23234102 | MS.gene90467:CDS |
| GATTCGATGCTTGGGACTGT+GGG | 0.480469 | 8.3:+23234103 | MS.gene90467:CDS |
| ATTCCGGCTAAGCATTCATC+AGG | 0.481371 | 8.3:-23233476 | None:intergenic |
| CGGGGTGGCTGCGGTTAAAG+AGG | 0.481372 | 8.3:+23234653 | MS.gene90467:CDS |
| GTTTGAAGGAGCTTGTTAAC+GGG | 0.482003 | 8.3:+23234005 | MS.gene90467:CDS |
| TCGAAGGAGAATCATCGATA+AGG | 0.483432 | 8.3:-23234792 | None:intergenic |
| CTTTCTGTTAAGAGGCTTCG+TGG | 0.487559 | 8.3:+23233902 | MS.gene90467:CDS |
| ATTGCTCCGCCGTGTGCAAA+CGG | 0.487943 | 8.3:+23233531 | MS.gene90467:CDS |
| TCCTAATTTGGTTAAACTCA+AGG | 0.488244 | 8.3:+23234597 | MS.gene90467:CDS |
| TAATGAGGAAGGTGTTGAAT+CGG | 0.493143 | 8.3:+23233946 | MS.gene90467:CDS |
| GTAGGGAAGTTACTGAGATT+GGG | 0.496163 | 8.3:+23233765 | MS.gene90467:CDS |
| CGTGTTGTCTGAGTTTGGAA+AGG | 0.499057 | 8.3:+23234437 | MS.gene90467:CDS |
| ACCAATTATCAGCGGAGCAA+AGG | 0.500159 | 8.3:-23234032 | None:intergenic |
| CATCAACGGTGTGAATTACT+CGG | 0.507077 | 8.3:+23233445 | MS.gene90467:CDS |
| GATGGAAGTGGTAGTGATGT+TGG | 0.507736 | 8.3:+23234721 | MS.gene90467:CDS |
| TCGTGCCGTGTGCGTTCAGA+AGG | 0.508031 | 8.3:+23234884 | MS.gene90467:CDS |
| GCCGTGTGCGTTCAGAAGGT+GGG | 0.508617 | 8.3:+23234888 | MS.gene90467:CDS |
| ACTTCCTCGACGCCGGCGAT+CGG | 0.511869 | 8.3:+23233504 | MS.gene90467:CDS |
| GGTTGAGTTTCATCTTGAGA+AGG | 0.513025 | 8.3:+23234144 | MS.gene90467:CDS |
| GCTTGAAGCTTCGCGCGTGT+AGG | 0.518064 | 8.3:+23233747 | MS.gene90467:CDS |
| GGGGAAGATGAATTCTGGGT+TGG | 0.523082 | 8.3:+23234123 | MS.gene90467:CDS |
| TCTTCCGATGATTTCATCAA+CGG | 0.525072 | 8.3:+23233431 | MS.gene90467:CDS |
| ACACCCGAGTGTTCAGATCA+TGG | 0.528481 | 8.3:+23234238 | MS.gene90467:CDS |
| ATTCCTGATGAATGCTTAGC+CGG | 0.533855 | 8.3:+23233473 | MS.gene90467:CDS |
| AAAGTTACTGGTGAAGCAGC+GGG | 0.535196 | 8.3:+23234634 | MS.gene90467:CDS |
| CGTTTAGTGAAAGCCGCTGT+CGG | 0.538176 | 8.3:-23233577 | None:intergenic |
| AGCTTCACATTGATGGGTGG+AGG | 0.538707 | 8.3:+23234299 | MS.gene90467:CDS |
| ATCTGGTGACGGTGATGGAA+CGG | 0.540576 | 8.3:-23233366 | None:intergenic |
| GTTACATCTGGTGACGGTGA+TGG | 0.542980 | 8.3:-23233371 | None:intergenic |
| GCCCACCTTCTGAACGCACA+CGG | 0.545127 | 8.3:-23234889 | None:intergenic |
| TGGTCCTGAACATCGACAAT+CGG | 0.547994 | 8.3:-23234834 | None:intergenic |
| GAAGGTGTTGAATCGGTTTG+TGG | 0.549675 | 8.3:+23233953 | MS.gene90467:CDS |
| TGATAATAGTAATGTTCTTG+AGG | 0.553275 | 8.3:+23233877 | MS.gene90467:CDS |
| GGTGATGGAACGGCGGTTGA+CGG | 0.553450 | 8.3:-23233356 | None:intergenic |
| GATGTAACCCGATTCCGTTA+CGG | 0.558766 | 8.3:+23233386 | MS.gene90467:CDS |
| GGTTGTCCTGTGACTAATGT+CGG | 0.559138 | 8.3:+23234550 | MS.gene90467:CDS |
| AAGACCATGATCTGAACACT+CGG | 0.568218 | 8.3:-23234242 | None:intergenic |
| AAGGTTCAAGTTAGTGATGT+GGG | 0.569208 | 8.3:+23234163 | MS.gene90467:CDS |
| ACTAACAGAATTGGTGATGA+GGG | 0.572704 | 8.3:+23234322 | MS.gene90467:CDS |
| AGTCAAGTTGAAGCAATGGA+TGG | 0.579018 | 8.3:+23234703 | MS.gene90467:CDS |
| GTTGAAGCAATGGATGGAAG+TGG | 0.579481 | 8.3:+23234709 | MS.gene90467:CDS |
| TCATAGTCAAGTTGAAGCAA+TGG | 0.585147 | 8.3:+23234699 | MS.gene90467:CDS |
| GGGTGGAGGACTAACAGAAT+TGG | 0.585691 | 8.3:+23234313 | MS.gene90467:CDS |
| TTGTTGTTGTTACTACTCGA+AGG | 0.592454 | 8.3:-23234808 | None:intergenic |
| GTTAACGAGGTTAATGAGGA+AGG | 0.608011 | 8.3:+23233935 | MS.gene90467:CDS |
| GACTAACAGAATTGGTGATG+AGG | 0.609807 | 8.3:+23234321 | MS.gene90467:CDS |
| GTCGGCTTTCACCTTCCACA+CGG | 0.614416 | 8.3:-23233559 | None:intergenic |
| GAAGGTTCAAGTTAGTGATG+TGG | 0.615255 | 8.3:+23234162 | MS.gene90467:CDS |
| ACGGAACCACCGTTTGCACA+CGG | 0.617375 | 8.3:-23233540 | None:intergenic |
| AAACGGTGGTTCCGTGTGGA+AGG | 0.620068 | 8.3:+23233548 | MS.gene90467:CDS |
| CACACCGTTGATGAAATCAT+CGG | 0.627853 | 8.3:-23233435 | None:intergenic |
| GAAAGTTACTGGTGAAGCAG+CGG | 0.628253 | 8.3:+23234633 | MS.gene90467:CDS |
| GCTCCGCCGTGTGCAAACGG+TGG | 0.640624 | 8.3:+23233534 | MS.gene90467:CDS |
| ATTCGATGCTTGGGACTGTG+GGG | 0.642720 | 8.3:+23234104 | MS.gene90467:CDS |
| AAAAGCTTCACATTGATGGG+TGG | 0.648601 | 8.3:+23234296 | MS.gene90467:CDS |
| GCAATGCCGACATTAGTCAC+AGG | 0.649753 | 8.3:-23234556 | None:intergenic |
| ATACTTCACTTCCTCGACGC+CGG | 0.658405 | 8.3:+23233497 | MS.gene90467:CDS |
| TGGTGTTAACGATGTTAACG+AGG | 0.658813 | 8.3:+23233922 | MS.gene90467:CDS |
| TCGTAACGATTGAACAACGA+AGG | 0.658946 | 8.3:-23233626 | None:intergenic |
| GTGCAAACGGTGGTTCCGTG+TGG | 0.673997 | 8.3:+23233544 | MS.gene90467:CDS |
| TGGAAGGTGAAAGCCGACAG+CGG | 0.678069 | 8.3:+23233564 | MS.gene90467:CDS |
| TTTCTCGAACCAATTATCAG+CGG | 0.683028 | 8.3:-23234040 | None:intergenic |
| AGACCATGATCTGAACACTC+GGG | 0.683312 | 8.3:-23234241 | None:intergenic |
| AATCGGGTTACATCTGGTGA+CGG | 0.684512 | 8.3:-23233377 | None:intergenic |
| ACAGCGGCTTTCACTAAACG+CGG | 0.700210 | 8.3:+23233580 | MS.gene90467:CDS |
| TGGAGTTCAAGAAACTACCA+TGG | 0.701774 | 8.3:+23234741 | MS.gene90467:CDS |
| TTACTGGTGAAGCAGCGGGG+TGG | 0.704340 | 8.3:+23234638 | MS.gene90467:CDS |
| GCGGCTTTCACTAAACGCGG+AGG | 0.706709 | 8.3:+23233583 | MS.gene90467:CDS |
| GCTCCCAAACTCAACGACGT+AGG | 0.707891 | 8.3:-23234406 | None:intergenic |
| AAGTTACTGGTGAAGCAGCG+GGG | 0.729945 | 8.3:+23234635 | MS.gene90467:CDS |
| CAACGGTGTGAATTACTCGG+AGG | 0.735967 | 8.3:+23233448 | MS.gene90467:CDS |
| TGGTGACGGTGATGGAACGG+CGG | 0.753287 | 8.3:-23233363 | None:intergenic |
| GAACCACCGTTTGCACACGG+CGG | 0.791657 | 8.3:-23233537 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTTAAGAAATTGTGCATTA+AGG | + | chr8.3:23234528-23234547 | MS.gene90467:CDS | 25.0% |
| ! | CTTAAGAAATTGTGCATTAA+GGG | + | chr8.3:23234529-23234548 | MS.gene90467:CDS | 25.0% |
| ! | TAAGAATTTGAAGAAGCTTT+CGG | + | chr8.3:23233811-23233830 | MS.gene90467:CDS | 25.0% |
| ! | TGATAATAGTAATGTTCTTG+AGG | + | chr8.3:23233877-23233896 | MS.gene90467:CDS | 25.0% |
| !! | GATTTCAAAGTGTTTGAAAT+TGG | + | chr8.3:23234195-23234214 | MS.gene90467:CDS | 25.0% |
| ACCTTGAGTTTAACCAAATT+AGG | - | chr8.3:23234601-23234620 | None:intergenic | 30.0% | |
| TCCTAATTTGGTTAAACTCA+AGG | + | chr8.3:23234597-23234616 | MS.gene90467:CDS | 30.0% | |
| TGAAAAAGCTTCACATTGAT+GGG | + | chr8.3:23234293-23234312 | MS.gene90467:CDS | 30.0% | |
| TTGAAAAAGCTTCACATTGA+TGG | + | chr8.3:23234292-23234311 | MS.gene90467:CDS | 30.0% | |
| !! | TTCAAACTTTGAAGCTGATT+CGG | + | chr8.3:23234065-23234084 | MS.gene90467:CDS | 30.0% |
| TCTTCCGATGATTTCATCAA+CGG | + | chr8.3:23233431-23233450 | MS.gene90467:CDS | 35.0% | |
| TTACTGAGATTGGGATGTTA+GGG | + | chr8.3:23233774-23233793 | MS.gene90467:CDS | 35.0% | |
| TTGAGGAACTTTCTGTTAAG+AGG | + | chr8.3:23233894-23233913 | MS.gene90467:CDS | 35.0% | |
| TTTCTCGAACCAATTATCAG+CGG | - | chr8.3:23234043-23234062 | None:intergenic | 35.0% | |
| ! | AAGGTTCAAGTTAGTGATGT+GGG | + | chr8.3:23234163-23234182 | MS.gene90467:CDS | 35.0% |
| ! | AGAAAGTGTGTGAAAGTTAC+TGG | + | chr8.3:23234622-23234641 | MS.gene90467:CDS | 35.0% |
| ! | TTGTTGTTGTTACTACTCGA+AGG | - | chr8.3:23234811-23234830 | None:intergenic | 35.0% |
| !! | ACTAACAGAATTGGTGATGA+GGG | + | chr8.3:23234322-23234341 | MS.gene90467:CDS | 35.0% |
| !! | GTTGAGATCGATTTGTTTGA+AGG | + | chr8.3:23233991-23234010 | MS.gene90467:CDS | 35.0% |
| !! | TAATGAGGAAGGTGTTGAAT+CGG | + | chr8.3:23233946-23233965 | MS.gene90467:CDS | 35.0% |
| !! | TCATAGTCAAGTTGAAGCAA+TGG | + | chr8.3:23234699-23234718 | MS.gene90467:CDS | 35.0% |
| !! | TCGTGTTTGTTTGGTGTTAA+GGG | + | chr8.3:23233839-23233858 | MS.gene90467:CDS | 35.0% |
| !! | TGTTTGAAGGAGCTTGTTAA+CGG | + | chr8.3:23234004-23234023 | MS.gene90467:CDS | 35.0% |
| !! | TTCGTGTTTGTTTGGTGTTA+AGG | + | chr8.3:23233838-23233857 | MS.gene90467:CDS | 35.0% |
| !! | TTGAATCGGTTTGTGGTAAT+GGG | + | chr8.3:23233960-23233979 | MS.gene90467:CDS | 35.0% |
| AAAAGCTTCACATTGATGGG+TGG | + | chr8.3:23234296-23234315 | MS.gene90467:CDS | 40.0% | |
| AACACAAGCTCTTGCAAGTT+AGG | - | chr8.3:23234373-23234392 | None:intergenic | 40.0% | |
| AAGACCATGATCTGAACACT+CGG | - | chr8.3:23234245-23234264 | None:intergenic | 40.0% | |
| ACCAGCCAAAAATCCAAACT+TGG | - | chr8.3:23234857-23234876 | None:intergenic | 40.0% | |
| ATAACCGATTGTCGATGTTC+AGG | + | chr8.3:23234830-23234849 | MS.gene90467:CDS | 40.0% | |
| ATTCCTGATGAATGCTTAGC+CGG | + | chr8.3:23233473-23233492 | MS.gene90467:CDS | 40.0% | |
| CATCAACGGTGTGAATTACT+CGG | + | chr8.3:23233445-23233464 | MS.gene90467:CDS | 40.0% | |
| CGAAGGAGAATCATCGATAA+GGG | - | chr8.3:23234794-23234813 | None:intergenic | 40.0% | |
| CGATGTTAACGAGGTTAATG+AGG | + | chr8.3:23233931-23233950 | MS.gene90467:CDS | 40.0% | |
| GGTTGAGTTTCATCTTGAGA+AGG | + | chr8.3:23234144-23234163 | MS.gene90467:CDS | 40.0% | |
| GTAGGGAAGTTACTGAGATT+GGG | + | chr8.3:23233765-23233784 | MS.gene90467:CDS | 40.0% | |
| GTTAACGAGGTTAATGAGGA+AGG | + | chr8.3:23233935-23233954 | MS.gene90467:CDS | 40.0% | |
| GTTACTGAGATTGGGATGTT+AGG | + | chr8.3:23233773-23233792 | MS.gene90467:CDS | 40.0% | |
| TATCCTACGTCGTTGAGTTT+GGG | + | chr8.3:23234403-23234422 | MS.gene90467:CDS | 40.0% | |
| TCGAAGGAGAATCATCGATA+AGG | - | chr8.3:23234795-23234814 | None:intergenic | 40.0% | |
| TCGTAACGATTGAACAACGA+AGG | - | chr8.3:23233629-23233648 | None:intergenic | 40.0% | |
| TGGAGTTCAAGAAACTACCA+TGG | + | chr8.3:23234741-23234760 | MS.gene90467:CDS | 40.0% | |
| TGTAGGGAAGTTACTGAGAT+TGG | + | chr8.3:23233764-23233783 | MS.gene90467:CDS | 40.0% | |
| ! | CACACCGTTGATGAAATCAT+CGG | - | chr8.3:23233438-23233457 | None:intergenic | 40.0% |
| ! | CAGGACCAAGTTTGGATTTT+TGG | + | chr8.3:23234849-23234868 | MS.gene90467:CDS | 40.0% |
| ! | GAAGGTTCAAGTTAGTGATG+TGG | + | chr8.3:23234162-23234181 | MS.gene90467:CDS | 40.0% |
| ! | TCGGTTGTTTCGTGTTTGTT+TGG | + | chr8.3:23233830-23233849 | MS.gene90467:CDS | 40.0% |
| ! | TGGAAAGGTTTGCTCTTTGT+GGG | + | chr8.3:23234452-23234471 | MS.gene90467:CDS | 40.0% |
| ! | TGGTGTTAACGATGTTAACG+AGG | + | chr8.3:23233922-23233941 | MS.gene90467:CDS | 40.0% |
| ! | TTGGAAAGGTTTGCTCTTTG+TGG | + | chr8.3:23234451-23234470 | MS.gene90467:CDS | 40.0% |
| !! | AGTCAAGTTGAAGCAATGGA+TGG | + | chr8.3:23234703-23234722 | MS.gene90467:CDS | 40.0% |
| !! | GACTAACAGAATTGGTGATG+AGG | + | chr8.3:23234321-23234340 | MS.gene90467:CDS | 40.0% |
| !! | GATTCGGTGTATTGGTGATT+GGG | + | chr8.3:23234081-23234100 | MS.gene90467:CDS | 40.0% |
| !! | GTCATTTTGCAGAAAAGCCA+TGG | - | chr8.3:23234761-23234780 | None:intergenic | 40.0% |
| !! | GTTGAATCGGTTTGTGGTAA+TGG | + | chr8.3:23233959-23233978 | MS.gene90467:CDS | 40.0% |
| !! | GTTTGAAGGAGCTTGTTAAC+GGG | + | chr8.3:23234005-23234024 | MS.gene90467:CDS | 40.0% |
| !! | TGATTCGGTGTATTGGTGAT+TGG | + | chr8.3:23234080-23234099 | MS.gene90467:CDS | 40.0% |
| !! | TTAGTGATGTGGGTCTTGTT+GGG | + | chr8.3:23234173-23234192 | MS.gene90467:CDS | 40.0% |
| !! | TTGAAGCTGATTCGGTGTAT+TGG | + | chr8.3:23234073-23234092 | MS.gene90467:CDS | 40.0% |
| !!! | ACCAAGTTTGGATTTTTGGC+TGG | + | chr8.3:23234853-23234872 | MS.gene90467:CDS | 40.0% |
| !!! | AGTTTGGATTTTTGGCTGGT+AGG | + | chr8.3:23234857-23234876 | MS.gene90467:CDS | 40.0% |
| AAAGTTACTGGTGAAGCAGC+GGG | + | chr8.3:23234634-23234653 | MS.gene90467:CDS | 45.0% | |
| AATCGGGTTACATCTGGTGA+CGG | - | chr8.3:23233380-23233399 | None:intergenic | 45.0% | |
| ACCAATTATCAGCGGAGCAA+AGG | - | chr8.3:23234035-23234054 | None:intergenic | 45.0% | |
| ACTGTGGGGAAGATGAATTC+TGG | + | chr8.3:23234118-23234137 | MS.gene90467:CDS | 45.0% | |
| AGACCATGATCTGAACACTC+GGG | - | chr8.3:23234244-23234263 | None:intergenic | 45.0% | |
| ATTCCGGCTAAGCATTCATC+AGG | - | chr8.3:23233479-23233498 | None:intergenic | 45.0% | |
| CGTGTTGTCTGAGTTTGGAA+AGG | + | chr8.3:23234437-23234456 | MS.gene90467:CDS | 45.0% | |
| CTGTGGGGAAGATGAATTCT+GGG | + | chr8.3:23234119-23234138 | MS.gene90467:CDS | 45.0% | |
| CTTTCTGTTAAGAGGCTTCG+TGG | + | chr8.3:23233902-23233921 | MS.gene90467:CDS | 45.0% | |
| GAAAGTTACTGGTGAAGCAG+CGG | + | chr8.3:23234633-23234652 | MS.gene90467:CDS | 45.0% | |
| GATGTAACCCGATTCCGTTA+CGG | + | chr8.3:23233386-23233405 | MS.gene90467:CDS | 45.0% | |
| GCGGTTAAAGAGGAGTTCTT+TGG | + | chr8.3:23234663-23234682 | MS.gene90467:CDS | 45.0% | |
| GGTTGTCCTGTGACTAATGT+CGG | + | chr8.3:23234550-23234569 | MS.gene90467:CDS | 45.0% | |
| GTATCCTACGTCGTTGAGTT+TGG | + | chr8.3:23234402-23234421 | MS.gene90467:CDS | 45.0% | |
| GTGATTGGGATTCGATGCTT+GGG | + | chr8.3:23234095-23234114 | MS.gene90467:CDS | 45.0% | |
| GTTGAAGCAATGGATGGAAG+TGG | + | chr8.3:23234709-23234728 | MS.gene90467:CDS | 45.0% | |
| TAACGGAATCGGGTTACATC+TGG | - | chr8.3:23233386-23233405 | None:intergenic | 45.0% | |
| TCCTTTGCTCCGCTGATAAT+TGG | + | chr8.3:23234031-23234050 | MS.gene90467:CDS | 45.0% | |
| TCGATGTTCAGGACCAAGTT+TGG | + | chr8.3:23234841-23234860 | MS.gene90467:CDS | 45.0% | |
| TGCTTCGTGTTGTCTGAGTT+TGG | + | chr8.3:23234432-23234451 | MS.gene90467:CDS | 45.0% | |
| TGCTTCTGGGTGTCCTAATT+TGG | + | chr8.3:23234585-23234604 | MS.gene90467:CDS | 45.0% | |
| TGGTCCTGAACATCGACAAT+CGG | - | chr8.3:23234837-23234856 | None:intergenic | 45.0% | |
| ! | AGGTTTGCTCTTTGTGGGAT+TGG | + | chr8.3:23234457-23234476 | MS.gene90467:CDS | 45.0% |
| ! | GATGGAAGTGGTAGTGATGT+TGG | + | chr8.3:23234721-23234740 | MS.gene90467:CDS | 45.0% |
| ! | GTCGAGGAAGTGAAGTATTC+CGG | - | chr8.3:23233495-23233514 | None:intergenic | 45.0% |
| ! | GTTAGTGATGTGGGTCTTGT+TGG | + | chr8.3:23234172-23234191 | MS.gene90467:CDS | 45.0% |
| ! | TGGTGATGAGGGTTTGGTTT+CGG | + | chr8.3:23234333-23234352 | MS.gene90467:CDS | 45.0% |
| ! | TTGTTATTGCTGAGCGTTGC+AGG | + | chr8.3:23234266-23234285 | MS.gene90467:CDS | 45.0% |
| !! | CAGAATTGGTGATGAGGGTT+TGG | + | chr8.3:23234327-23234346 | MS.gene90467:CDS | 45.0% |
| !! | GAAGGTGTTGAATCGGTTTG+TGG | + | chr8.3:23233953-23233972 | MS.gene90467:CDS | 45.0% |
| !!! | GCATTGCTGCTTTTGCTTCT+GGG | + | chr8.3:23234572-23234591 | MS.gene90467:CDS | 45.0% |
| AAGTTACTGGTGAAGCAGCG+GGG | + | chr8.3:23234635-23234654 | MS.gene90467:CDS | 50.0% | |
| ACACCCGAGTGTTCAGATCA+TGG | + | chr8.3:23234238-23234257 | MS.gene90467:CDS | 50.0% | |
| ACAGCGGCTTTCACTAAACG+CGG | + | chr8.3:23233580-23233599 | MS.gene90467:CDS | 50.0% | |
| ACTAAACGCGGAGGCGAAAT+TGG | + | chr8.3:23233592-23233611 | MS.gene90467:CDS | 50.0% | |
| ATACTTCACTTCCTCGACGC+CGG | + | chr8.3:23233497-23233516 | MS.gene90467:CDS | 50.0% | |
| ATTCGATGCTTGGGACTGTG+GGG | + | chr8.3:23234104-23234123 | MS.gene90467:CDS | 50.0% | |
| CAACGGTGTGAATTACTCGG+AGG | + | chr8.3:23233448-23233467 | MS.gene90467:CDS | 50.0% | |
| CGTTTAGTGAAAGCCGCTGT+CGG | - | chr8.3:23233580-23233599 | None:intergenic | 50.0% | |
| GATTCGATGCTTGGGACTGT+GGG | + | chr8.3:23234103-23234122 | MS.gene90467:CDS | 50.0% | |
| GCAATGCCGACATTAGTCAC+AGG | - | chr8.3:23234559-23234578 | None:intergenic | 50.0% | |
| GGGGAAGATGAATTCTGGGT+TGG | + | chr8.3:23234123-23234142 | MS.gene90467:CDS | 50.0% | |
| GGGTGGAGGACTAACAGAAT+TGG | + | chr8.3:23234313-23234332 | MS.gene90467:CDS | 50.0% | |
| GGTGATTGGGATTCGATGCT+TGG | + | chr8.3:23234094-23234113 | MS.gene90467:CDS | 50.0% | |
| TACCGTTTCCGTTGCCGTAA+CGG | - | chr8.3:23233403-23233422 | None:intergenic | 50.0% | |
| TTCCGTTACGGCAACGGAAA+CGG | + | chr8.3:23233398-23233417 | MS.gene90467:CDS | 50.0% | |
| ! | AGCTTCACATTGATGGGTGG+AGG | + | chr8.3:23234299-23234318 | MS.gene90467:CDS | 50.0% |
| ! | GGAGCAATTTTTCCGATCGC+CGG | - | chr8.3:23233519-23233538 | None:intergenic | 50.0% |
| ! | TTCCGTTGCCGTAACGGAAT+CGG | - | chr8.3:23233397-23233416 | None:intergenic | 50.0% |
| !! | ATCTGGTGACGGTGATGGAA+CGG | - | chr8.3:23233369-23233388 | None:intergenic | 50.0% |
| !! | GTTACATCTGGTGACGGTGA+TGG | - | chr8.3:23233374-23233393 | None:intergenic | 50.0% |
| !!! | GGCATTGCTGCTTTTGCTTC+TGG | + | chr8.3:23234571-23234590 | MS.gene90467:CDS | 50.0% |
| AAACGGTGGTTCCGTGTGGA+AGG | + | chr8.3:23233548-23233567 | MS.gene90467:CDS | 55.0% | |
| ACCCGATTCCGTTACGGCAA+CGG | + | chr8.3:23233392-23233411 | MS.gene90467:CDS | 55.0% | |
| ACGGAACCACCGTTTGCACA+CGG | - | chr8.3:23233543-23233562 | None:intergenic | 55.0% | |
| ATTGCTCCGCCGTGTGCAAA+CGG | + | chr8.3:23233531-23233550 | MS.gene90467:CDS | 55.0% | |
| CTTGAAGCTTCGCGCGTGTA+GGG | + | chr8.3:23233748-23233767 | MS.gene90467:CDS | 55.0% | |
| GCTCCCAAACTCAACGACGT+AGG | - | chr8.3:23234409-23234428 | None:intergenic | 55.0% | |
| GGATTCGATGCTTGGGACTG+TGG | + | chr8.3:23234102-23234121 | MS.gene90467:CDS | 55.0% | |
| TGGAAGGTGAAAGCCGACAG+CGG | + | chr8.3:23233564-23233583 | MS.gene90467:CDS | 55.0% | |
| ! | GTCGGCTTTCACCTTCCACA+CGG | - | chr8.3:23233562-23233581 | None:intergenic | 55.0% |
| ! | TCCGTTGCCGTAACGGAATC+GGG | - | chr8.3:23233396-23233415 | None:intergenic | 55.0% |
| GAACCACCGTTTGCACACGG+CGG | - | chr8.3:23233540-23233559 | None:intergenic | 60.0% | |
| GCCCACCTTCTGAACGCACA+CGG | - | chr8.3:23234892-23234911 | None:intergenic | 60.0% | |
| GCCGTGTGCGTTCAGAAGGT+GGG | + | chr8.3:23234888-23234907 | MS.gene90467:CDS | 60.0% | |
| GCGGCTTTCACTAAACGCGG+AGG | + | chr8.3:23233583-23233602 | MS.gene90467:CDS | 60.0% | |
| GCTTGAAGCTTCGCGCGTGT+AGG | + | chr8.3:23233747-23233766 | MS.gene90467:CDS | 60.0% | |
| GTGCAAACGGTGGTTCCGTG+TGG | + | chr8.3:23233544-23233563 | MS.gene90467:CDS | 60.0% | |
| TCGTGCCGTGTGCGTTCAGA+AGG | + | chr8.3:23234884-23234903 | MS.gene90467:CDS | 60.0% | |
| TGCCGTGTGCGTTCAGAAGG+TGG | + | chr8.3:23234887-23234906 | MS.gene90467:CDS | 60.0% | |
| TTACTGGTGAAGCAGCGGGG+TGG | + | chr8.3:23234638-23234657 | MS.gene90467:CDS | 60.0% | |
| !! | GGTGATGGAACGGCGGTTGA+CGG | - | chr8.3:23233359-23233378 | None:intergenic | 60.0% |
| !! | TGGTGACGGTGATGGAACGG+CGG | - | chr8.3:23233366-23233385 | None:intergenic | 60.0% |
| ACTTCCTCGACGCCGGCGAT+CGG | + | chr8.3:23233504-23233523 | MS.gene90467:CDS | 65.0% | |
| CGGGGTGGCTGCGGTTAAAG+AGG | + | chr8.3:23234653-23234672 | MS.gene90467:CDS | 65.0% | |
| ! | TTTTCCGATCGCCGGCGTCG+AGG | - | chr8.3:23233511-23233530 | None:intergenic | 65.0% |
| GCTCCGCCGTGTGCAAACGG+TGG | + | chr8.3:23233534-23233553 | MS.gene90467:CDS | 70.0% | |
| GTGAAGCAGCGGGGTGGCTG+CGG | + | chr8.3:23234644-23234663 | MS.gene90467:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 23233344 | 23234951 | 23233344 | ID=MS.gene90467 |
| chr8.3 | mRNA | 23233344 | 23234951 | 23233344 | ID=MS.gene90467.t1;Parent=MS.gene90467 |
| chr8.3 | exon | 23233344 | 23234951 | 23233344 | ID=MS.gene90467.t1.exon1;Parent=MS.gene90467.t1 |
| chr8.3 | CDS | 23233344 | 23234951 | 23233344 | ID=cds.MS.gene90467.t1;Parent=MS.gene90467.t1 |
| Gene Sequence |
| Protein sequence |
