Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91127.t1 | XP_024640266.1 | 99.2 | 362 | 3 | 0 | 63 | 424 | 66 | 427 | 6.10E-205 | 723.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91127.t1 | Q08A71 | 78.4 | 361 | 74 | 2 | 68 | 424 | 75 | 435 | 9.1e-165 | 581.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91127.t1 | A0A2Z6MTP5 | 93.9 | 360 | 16 | 1 | 65 | 424 | 74 | 427 | 5.1e-193 | 683.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene067338 | MS.gene91127 | PPI |
| MS.gene064036 | MS.gene91127 | PPI |
| MS.gene91127 | MS.gene030293 | PPI |
| MS.gene66341 | MS.gene91127 | PPI |
| MS.gene038866 | MS.gene91127 | PPI |
| MS.gene75060 | MS.gene91127 | PPI |
| MS.gene75062 | MS.gene91127 | PPI |
| MS.gene68888 | MS.gene91127 | PPI |
| MS.gene91127 | MS.gene64396 | PPI |
| MS.gene030293 | MS.gene91127 | PPI |
| MS.gene83327 | MS.gene91127 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91127.t1 | MTR_1g113940 | 52.793 | 358 | 124 | 6 | 75 | 419 | 11 | 336 | 3.41e-120 | 353 |
| MS.gene91127.t1 | MTR_6g091770 | 38.636 | 352 | 177 | 6 | 65 | 415 | 57 | 370 | 6.30e-79 | 249 |
| MS.gene91127.t1 | MTR_2g054880 | 37.709 | 358 | 171 | 8 | 76 | 410 | 26 | 354 | 3.25e-63 | 208 |
| MS.gene91127.t1 | MTR_3g013960 | 38.393 | 336 | 149 | 8 | 75 | 382 | 255 | 560 | 7.31e-60 | 206 |
| MS.gene91127.t1 | MTR_4g119900 | 36.364 | 341 | 174 | 8 | 76 | 408 | 142 | 447 | 2.39e-56 | 194 |
| MS.gene91127.t1 | MTR_4g119900 | 36.364 | 341 | 174 | 8 | 76 | 408 | 142 | 447 | 2.59e-56 | 194 |
| MS.gene91127.t1 | MTR_2g436780 | 35.692 | 325 | 172 | 6 | 76 | 394 | 144 | 437 | 8.85e-55 | 190 |
| MS.gene91127.t1 | MTR_2g436780 | 35.692 | 325 | 172 | 6 | 76 | 394 | 144 | 437 | 8.98e-55 | 190 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91127.t1 | AT3G20020 | 69.284 | 433 | 107 | 7 | 12 | 424 | 9 | 435 | 0.0 | 608 |
| MS.gene91127.t1 | AT3G20020 | 68.310 | 426 | 109 | 7 | 6 | 411 | 3 | 422 | 0.0 | 588 |
| MS.gene91127.t1 | AT3G20020 | 64.896 | 433 | 104 | 8 | 12 | 424 | 9 | 413 | 0.0 | 559 |
| MS.gene91127.t1 | AT2G19670 | 39.466 | 337 | 168 | 5 | 76 | 411 | 48 | 349 | 1.71e-82 | 258 |
| MS.gene91127.t1 | AT4G29510 | 37.892 | 351 | 179 | 6 | 62 | 411 | 61 | 373 | 4.81e-81 | 255 |
| MS.gene91127.t1 | AT3G12270 | 38.356 | 365 | 178 | 11 | 41 | 390 | 212 | 544 | 1.04e-66 | 223 |
| MS.gene91127.t1 | AT1G04870 | 37.079 | 356 | 172 | 10 | 76 | 408 | 35 | 361 | 5.47e-66 | 216 |
| MS.gene91127.t1 | AT3G06930 | 35.692 | 325 | 172 | 6 | 76 | 394 | 148 | 441 | 6.31e-56 | 193 |
| MS.gene91127.t1 | AT3G06930 | 35.692 | 325 | 172 | 6 | 76 | 394 | 148 | 441 | 6.36e-56 | 193 |
| MS.gene91127.t1 | AT5G49020 | 33.923 | 339 | 187 | 6 | 76 | 408 | 151 | 458 | 1.29e-54 | 189 |
| MS.gene91127.t1 | AT5G49020 | 33.923 | 339 | 187 | 6 | 76 | 408 | 149 | 456 | 1.40e-54 | 189 |
| MS.gene91127.t1 | AT1G04870 | 34.843 | 287 | 135 | 10 | 145 | 408 | 1 | 258 | 8.16e-41 | 147 |
Find 95 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTCTTCTTCCATTGTT+AGG | 0.141991 | 1.3:+22190525 | MS.gene91127:CDS |
| GTGTGCGGACTGAAACTTAT+AGG | 0.254061 | 1.3:+22191908 | MS.gene91127:CDS |
| CGGTGTTGATGAATGATTAT+TGG | 0.306970 | 1.3:-22202046 | None:intergenic |
| TGATCTTGTTCCAGCTCAAT+AGG | 0.331644 | 1.3:-22202358 | None:intergenic |
| ACCGGCCTGAGCACAAAATA+TGG | 0.332954 | 1.3:-22192557 | None:intergenic |
| TACAGTTTCTAACTCATTGA+TGG | 0.342313 | 1.3:-22201637 | None:intergenic |
| TTCTTCTTCTTCCATTGTTA+GGG | 0.346521 | 1.3:+22190526 | MS.gene91127:CDS |
| ATTTGCTTTGACAACTTCAT+TGG | 0.373402 | 1.3:-22195721 | None:intergenic |
| TCCTTGCACTGATTACGATA+TGG | 0.382128 | 1.3:+22190601 | MS.gene91127:CDS |
| ACAGTGACAGCGTTGACTTT+TGG | 0.397498 | 1.3:+22198673 | MS.gene91127:CDS |
| ACCTGATGATTCTCAATAAC+CGG | 0.408242 | 1.3:-22202066 | None:intergenic |
| CAATGTGTTGGAGGGTCCTC+AGG | 0.428562 | 1.3:-22202150 | None:intergenic |
| CCATTCCTGACGGTGGTTGA+TGG | 0.433661 | 1.3:-22190448 | None:intergenic |
| GGTCCTCAGGAGCAGTAGAT+AGG | 0.435974 | 1.3:-22202137 | None:intergenic |
| ACCTGCTGCCAATGTGTTGG+AGG | 0.436915 | 1.3:-22202159 | None:intergenic |
| TATCTATCGGTATGTGTGAC+GGG | 0.443340 | 1.3:-22198651 | None:intergenic |
| AGGTTTGAGCCAGCGATCTC+TGG | 0.443984 | 1.3:-22198486 | None:intergenic |
| ATTATTGGTTGGTGCTATCA+TGG | 0.447582 | 1.3:-22202031 | None:intergenic |
| GTTGATGTTGGATGCGGTAC+AGG | 0.448555 | 1.3:+22192526 | MS.gene91127:CDS |
| TGCTCAGGCCGGTGCAAAGC+GGG | 0.449385 | 1.3:+22192567 | MS.gene91127:CDS |
| TGTGCGGACTGAAACTTATA+GGG | 0.452856 | 1.3:+22191909 | MS.gene91127:CDS |
| CCTGCCGTGGAGACGATAAC+TGG | 0.456845 | 1.3:+22199741 | MS.gene91127:CDS |
| GGGCTATATGCTTCTATATG+AGG | 0.459298 | 1.3:+22197025 | MS.gene91127:CDS |
| TGTTATAATTTCAGAATGGA+TGG | 0.463270 | 1.3:+22197004 | MS.gene91127:CDS |
| AGAAATACCGTTGCACTTGA+AGG | 0.467588 | 1.3:-22198524 | None:intergenic |
| CTTCAGGTCGTAGTTGATGT+TGG | 0.480894 | 1.3:+22192514 | MS.gene91127:intron |
| GAACAAGATCAAGTTATTGA+CGG | 0.493723 | 1.3:+22202370 | MS.gene91127:CDS |
| CCAGTTATCGTCTCCACGGC+AGG | 0.494216 | 1.3:-22199741 | None:intergenic |
| CCCTCCAACACATTGGCAGC+AGG | 0.494539 | 1.3:+22202158 | MS.gene91127:CDS |
| TTGCGGCGGTTGTTCTGTAT+CGG | 0.495312 | 1.3:-22190552 | None:intergenic |
| TGCACCTGCTGCCAATGTGT+TGG | 0.509483 | 1.3:-22202162 | None:intergenic |
| AGAGATCGCTGGCTCAAACC+TGG | 0.509562 | 1.3:+22198488 | MS.gene91127:CDS |
| GTATCTATCGGTATGTGTGA+CGG | 0.511759 | 1.3:-22198652 | None:intergenic |
| CACCACCATCAACCACCGTC+AGG | 0.512165 | 1.3:+22190443 | MS.gene91127:CDS |
| CTTCTATGATCCTATTGAGC+TGG | 0.512472 | 1.3:+22202348 | MS.gene91127:CDS |
| TTTCATTCCTATGCTCATCT+CGG | 0.515727 | 1.3:+22190629 | MS.gene91127:CDS |
| AGAGCATACCCGCTTTGCAC+CGG | 0.516717 | 1.3:-22192575 | None:intergenic |
| TACCTGCAGTGCAATGTCAC+TGG | 0.520846 | 1.3:-22193184 | None:intergenic |
| ACCGGTTATTGAGAATCATC+AGG | 0.527282 | 1.3:+22202065 | MS.gene91127:CDS |
| TGCCAGTGACATTGCACTGC+AGG | 0.527330 | 1.3:+22193182 | MS.gene91127:CDS |
| AAGAAGCGGAAGCGCGTGAA+CGG | 0.529510 | 1.3:-22190502 | None:intergenic |
| AGCGAATCCAAGTGAAGCAC+TGG | 0.531944 | 1.3:+22202113 | MS.gene91127:CDS |
| GATAGGACCAGTGCTTCACT+TGG | 0.532083 | 1.3:-22202120 | None:intergenic |
| TATTCATCAAGAAATGATCA+AGG | 0.539327 | 1.3:+22190652 | MS.gene91127:CDS |
| TTATTACTGCCAGAGATCGC+TGG | 0.544782 | 1.3:+22198477 | MS.gene91127:CDS |
| ATTGAGAATCATCAGGTGAA+TGG | 0.545325 | 1.3:+22202072 | MS.gene91127:CDS |
| TTCTAACTCATTGATGGTGA+CGG | 0.545702 | 1.3:-22201631 | None:intergenic |
| TATTCTTCCTTCAAGTGCAA+CGG | 0.546213 | 1.3:+22198517 | MS.gene91127:CDS |
| TGGATGTTATAATTTCAGAA+TGG | 0.561126 | 1.3:+22197000 | MS.gene91127:CDS |
| TGTATTGCATGGACGAGTTG+AGG | 0.564415 | 1.3:+22195764 | MS.gene91127:CDS |
| GTTATAATTTCAGAATGGAT+GGG | 0.566137 | 1.3:+22197005 | MS.gene91127:CDS |
| TCAGTGCAAGGAGATCGATT+AGG | 0.566907 | 1.3:-22190590 | None:intergenic |
| GTCGTAGTTGATGTTGGATG+CGG | 0.571602 | 1.3:+22192520 | MS.gene91127:CDS |
| CTGACGGTGGTTGATGGTGG+TGG | 0.572074 | 1.3:-22190442 | None:intergenic |
| CCATCAACCACCGTCAGGAA+TGG | 0.572345 | 1.3:+22190448 | MS.gene91127:CDS |
| CCTGCTGCCAATGTGTTGGA+GGG | 0.574057 | 1.3:-22202158 | None:intergenic |
| TGAACGGCGAGTGCCACGAC+GGG | 0.577599 | 1.3:-22190486 | None:intergenic |
| AACGCTGTCACTGTATCTAT+CGG | 0.578612 | 1.3:-22198664 | None:intergenic |
| GGTAGTTGTTGCTGCTGTTG+CGG | 0.581774 | 1.3:-22190569 | None:intergenic |
| ATCTATCGGTATGTGTGACG+GGG | 0.584626 | 1.3:-22198650 | None:intergenic |
| GGCGAGTGCCACGACGGGCA+CGG | 0.587048 | 1.3:-22190481 | None:intergenic |
| GGTCCTATCTACTGCTCCTG+AGG | 0.587466 | 1.3:+22202134 | MS.gene91127:CDS |
| GTTGATGAATGATTATTGGT+TGG | 0.587903 | 1.3:-22202042 | None:intergenic |
| CAAACTCACCGTGCCCGTCG+TGG | 0.590673 | 1.3:+22190473 | MS.gene91127:CDS |
| GATGTCGTCATTGTATTGCA+TGG | 0.590963 | 1.3:+22195753 | MS.gene91127:CDS |
| ATGAATACCGAGATGAGCAT+AGG | 0.591199 | 1.3:-22190636 | None:intergenic |
| CTGAGGACCCTCCAACACAT+TGG | 0.592000 | 1.3:+22202151 | MS.gene91127:CDS |
| TTCACCAGTTATCGTCTCCA+CGG | 0.592315 | 1.3:-22199745 | None:intergenic |
| GTGCTCAGGCCGGTGCAAAG+CGG | 0.594460 | 1.3:+22192566 | MS.gene91127:CDS |
| TTATTGGTTGGTGCTATCAT+GGG | 0.600178 | 1.3:-22202030 | None:intergenic |
| AAGGATAGTAACAGTAGTAA+CGG | 0.602378 | 1.3:+22190410 | MS.gene91127:CDS |
| ATCCGACTCACGTATACTCA+AGG | 0.615155 | 1.3:-22202444 | None:intergenic |
| TGTATCGGAAACCCTAACAA+TGG | 0.618088 | 1.3:-22190537 | None:intergenic |
| CCTGAGCACAAAATATGGAA+AGG | 0.619755 | 1.3:-22192552 | None:intergenic |
| TGCATTTGAAGAACCTGCCG+TGG | 0.621282 | 1.3:+22199728 | MS.gene91127:CDS |
| GCCATATCGTAATCAGTGCA+AGG | 0.625085 | 1.3:-22190602 | None:intergenic |
| CAATAATCATTCATCAACAC+CGG | 0.627958 | 1.3:+22202047 | MS.gene91127:CDS |
| CAAAATGCAAAACCATGCAG+TGG | 0.630371 | 1.3:-22201985 | None:intergenic |
| TGAGTTTGCACCATTCCTGA+CGG | 0.633140 | 1.3:-22190458 | None:intergenic |
| ATACAATGACGACATCAGAA+AGG | 0.634777 | 1.3:-22195746 | None:intergenic |
| GTTTGGTACAGGATCGTGTG+CGG | 0.635116 | 1.3:+22191893 | MS.gene91127:intron |
| GAAGGAAGAATAAGACCACC+AGG | 0.640805 | 1.3:-22198506 | None:intergenic |
| AGTTGTTGCTGCTGTTGCGG+CGG | 0.643125 | 1.3:-22190566 | None:intergenic |
| AGTAACAGTAGTAACGGTAA+CGG | 0.650759 | 1.3:+22190416 | MS.gene91127:CDS |
| GGATGTTGAAATCGATGAAG+AGG | 0.655942 | 1.3:+22196977 | MS.gene91127:intron |
| GTGAACGGCGAGTGCCACGA+CGG | 0.656629 | 1.3:-22190487 | None:intergenic |
| CGACTCACGTATACTCAAGG+TGG | 0.658948 | 1.3:-22202441 | None:intergenic |
| AAGTTCAACTCAATGATGCG+AGG | 0.662822 | 1.3:+22201672 | MS.gene91127:CDS |
| TTCCTGACGGTGGTTGATGG+TGG | 0.667258 | 1.3:-22190445 | None:intergenic |
| TGTTGAAATCGATGAAGAGG+TGG | 0.668496 | 1.3:+22196980 | MS.gene91127:CDS |
| ACTAGCATACTCACCACGTG+TGG | 0.671522 | 1.3:-22199783 | None:intergenic |
| GTTTGCACCATTCCTGACGG+TGG | 0.675193 | 1.3:-22190455 | None:intergenic |
| GATCGCTGGCTCAAACCTGG+TGG | 0.682355 | 1.3:+22198491 | MS.gene91127:CDS |
| TCTATCGGTATGTGTGACGG+GGG | 0.691322 | 1.3:-22198649 | None:intergenic |
| CTTATAGGGAAGCAATCATG+CGG | 0.705648 | 1.3:+22191923 | MS.gene91127:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 22190401 | 22202584 | 22190401 | ID=MS.gene91127 |
| chr1.3 | mRNA | 22190401 | 22202584 | 22190401 | ID=MS.gene91127.t1;Parent=MS.gene91127 |
| chr1.3 | exon | 22190401 | 22190673 | 22190401 | ID=MS.gene91127.t1.exon1;Parent=MS.gene91127.t1 |
| chr1.3 | CDS | 22190401 | 22190673 | 22190401 | ID=cds.MS.gene91127.t1;Parent=MS.gene91127.t1 |
| chr1.3 | exon | 22191904 | 22191969 | 22191904 | ID=MS.gene91127.t1.exon2;Parent=MS.gene91127.t1 |
| chr1.3 | CDS | 22191904 | 22191969 | 22191904 | ID=cds.MS.gene91127.t1;Parent=MS.gene91127.t1 |
| chr1.3 | exon | 22192520 | 22192588 | 22192520 | ID=MS.gene91127.t1.exon3;Parent=MS.gene91127.t1 |
| chr1.3 | CDS | 22192520 | 22192588 | 22192520 | ID=cds.MS.gene91127.t1;Parent=MS.gene91127.t1 |
| chr1.3 | exon | 22193168 | 22193203 | 22193168 | ID=MS.gene91127.t1.exon4;Parent=MS.gene91127.t1 |
| chr1.3 | CDS | 22193168 | 22193203 | 22193168 | ID=cds.MS.gene91127.t1;Parent=MS.gene91127.t1 |
| chr1.3 | exon | 22195720 | 22195785 | 22195720 | ID=MS.gene91127.t1.exon5;Parent=MS.gene91127.t1 |
| chr1.3 | CDS | 22195720 | 22195785 | 22195720 | ID=cds.MS.gene91127.t1;Parent=MS.gene91127.t1 |
| chr1.3 | exon | 22196978 | 22197046 | 22196978 | ID=MS.gene91127.t1.exon6;Parent=MS.gene91127.t1 |
| chr1.3 | CDS | 22196978 | 22197046 | 22196978 | ID=cds.MS.gene91127.t1;Parent=MS.gene91127.t1 |
| chr1.3 | exon | 22198461 | 22198538 | 22198461 | ID=MS.gene91127.t1.exon7;Parent=MS.gene91127.t1 |
| chr1.3 | CDS | 22198461 | 22198538 | 22198461 | ID=cds.MS.gene91127.t1;Parent=MS.gene91127.t1 |
| chr1.3 | exon | 22198639 | 22198717 | 22198639 | ID=MS.gene91127.t1.exon8;Parent=MS.gene91127.t1 |
| chr1.3 | CDS | 22198639 | 22198717 | 22198639 | ID=cds.MS.gene91127.t1;Parent=MS.gene91127.t1 |
| chr1.3 | exon | 22199697 | 22199791 | 22199697 | ID=MS.gene91127.t1.exon9;Parent=MS.gene91127.t1 |
| chr1.3 | CDS | 22199697 | 22199791 | 22199697 | ID=cds.MS.gene91127.t1;Parent=MS.gene91127.t1 |
| chr1.3 | exon | 22201609 | 22201693 | 22201609 | ID=MS.gene91127.t1.exon10;Parent=MS.gene91127.t1 |
| chr1.3 | CDS | 22201609 | 22201693 | 22201609 | ID=cds.MS.gene91127.t1;Parent=MS.gene91127.t1 |
| chr1.3 | exon | 22201983 | 22202179 | 22201983 | ID=MS.gene91127.t1.exon11;Parent=MS.gene91127.t1 |
| chr1.3 | CDS | 22201983 | 22202179 | 22201983 | ID=cds.MS.gene91127.t1;Parent=MS.gene91127.t1 |
| chr1.3 | exon | 22202337 | 22202455 | 22202337 | ID=MS.gene91127.t1.exon12;Parent=MS.gene91127.t1 |
| chr1.3 | CDS | 22202337 | 22202455 | 22202337 | ID=cds.MS.gene91127.t1;Parent=MS.gene91127.t1 |
| chr1.3 | exon | 22202542 | 22202584 | 22202542 | ID=MS.gene91127.t1.exon13;Parent=MS.gene91127.t1 |
| chr1.3 | CDS | 22202542 | 22202584 | 22202542 | ID=cds.MS.gene91127.t1;Parent=MS.gene91127.t1 |
| Gene Sequence |
| Protein sequence |
