AlfalfaGEDB | MS.gene91655

M. sativa cultivar XinJiangDaYe / MS.gene91655

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016757 GO:0016758
PFAM: PF00201.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91655.t1	XP_003621425.2	89.6	270	26	2	1	270	183	450	6.10E-142	513.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91655.t1	Q9SGA8	39.2	278	151	4	1	268	189	458	1.7e-60	234.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91655.t1	G7L593	89.6	270	26	2	1	270	173	440	4.4e-142	513.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene058781	MS.gene91655	0.811059	8.46E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene68301	MS.gene91655	PPI
MS.gene91655	MS.gene68300	PPI
MS.gene91655	MS.gene027547	PPI
MS.gene91655	MS.gene69282	PPI
MS.gene91655	MS.gene027549	PPI
MS.gene91655	MS.gene057369	PPI
MS.gene91655	MS.gene032032	PPI
MS.gene91655	MS.gene44914	PPI
MS.gene91655	MS.gene008107	PPI
MS.gene91655	MS.gene027548	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91655.t1	MTR_7g013200	89.630	270	26	2	1	270	173	440	1.41e-178	499
MS.gene91655.t1	MTR_7g013220	78.067	269	57	2	1	269	184	450	2.39e-156	443
MS.gene91655.t1	MTR_7g013240	70.260	269	78	2	1	269	173	439	1.09e-142	408
MS.gene91655.t1	MTR_7g013260	70.000	270	78	3	1	269	173	440	2.52e-141	404
MS.gene91655.t1	MTR_7g013180	57.249	269	112	1	1	269	179	444	2.98e-113	333
MS.gene91655.t1	MTR_6g038230	34.737	285	165	5	1	266	169	451	1.05e-48	167
MS.gene91655.t1	MTR_7g011470	39.149	235	125	6	46	266	228	458	2.18e-48	167
MS.gene91655.t1	MTR_7g011470	39.149	235	125	6	46	266	232	462	2.99e-48	166
MS.gene91655.t1	MTR_3g008010	35.484	279	155	7	9	266	200	474	9.25e-48	165
MS.gene91655.t1	MTR_1g107380	32.584	267	164	4	17	267	204	470	1.28e-47	165

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91655.t1	AT3G02100	39.568	278	150	5	1	268	189	458	5.91e-74	233
MS.gene91655.t1	AT5G59590	40.639	219	117	5	57	267	234	447	2.46e-50	171
MS.gene91655.t1	AT3G55700	36.842	285	158	8	1	270	174	451	3.49e-49	169
MS.gene91655.t1	AT5G05880	38.158	228	127	5	49	268	226	447	2.65e-48	166
MS.gene91655.t1	AT5G59580	36.471	255	144	6	26	267	196	445	1.53e-47	164
MS.gene91655.t1	AT3G46670	35.223	247	138	7	37	268	208	447	6.95e-47	162
MS.gene91655.t1	AT3G11340	34.875	281	171	5	1	270	166	445	1.08e-46	162
MS.gene91655.t1	AT1G22340	33.562	292	165	10	1	267	192	479	1.20e-45	160
MS.gene91655.t1	AT3G46680	33.929	280	156	9	4	267	181	447	1.75e-45	159
MS.gene91655.t1	AT3G21560	37.069	232	133	6	47	266	239	469	6.59e-45	158

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Find 57 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGAAGAGATAAAGAAGA+AGG	0.488960	7.4:+85992942	MS.gene91655:CDS
AAGAAGGTGGAACAATTGCT+TGG	0.525033	7.4:+85992958	MS.gene91655:CDS
AAGACATGACTTCCGTAGAT+TGG	0.464686	7.4:+85992506	MS.gene91655:CDS
AATTAGACAAGGATGAAAAT+GGG	0.477251	7.4:+85992911	MS.gene91655:CDS
AATTTAAAGAATTAACATTA+GGG	0.385494	7.4:+85992599	MS.gene91655:CDS
ACAGCAACAAAAGTTCATTC+TGG	0.367462	7.4:+85992479	MS.gene91655:CDS
ACATATACGACAGATTGAGG+CGG	0.741046	7.4:-85992541	None:intergenic
ACCAATTGGCCCATTGATGA+TGG	0.475756	7.4:+85992450	MS.gene91655:CDS
ACTATAGAAGGTGTATATTC+TGG	0.386931	7.4:+85992805	MS.gene91655:CDS
AGAAAGATCATTGAAACTAA+AGG	0.497391	7.4:+85992996	MS.gene91655:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGGAAGAGATAAAGAAGA+AGG	+	chr7.4:85992942-85992961	MS.gene91655:CDS	30.0%
AGTCATATATTTGTGATGTG+TGG	+	chr7.4:85992875-85992894	MS.gene91655:CDS	30.0%
ATATGTTTCCTTCGATAGTT+TGG	+	chr7.4:85992558-85992577	MS.gene91655:CDS	30.0%
ATCAAATACGATCTTGTCAA+AGG	-	chr7.4:85992326-85992345	None:intergenic	30.0%
GAATTAGACAAGGATGAAAA+TGG	+	chr7.4:85992910-85992929	MS.gene91655:CDS	30.0%
TAAAGGCAAGATTGTTAGTT+GGG	+	chr7.4:85992714-85992733	MS.gene91655:CDS	30.0%
TTGAATAACATAGAGGAAGA+TGG	+	chr7.4:85993027-85993046	MS.gene91655:CDS	30.0%
ACAGCAACAAAAGTTCATTC+TGG	+	chr7.4:85992479-85992498	MS.gene91655:CDS	35.0%
CTAAAGGCAAGATTGTTAGT+TGG	+	chr7.4:85992713-85992732	MS.gene91655:CDS	35.0%
CTAATGAAACAAGCTATAGC+AGG	-	chr7.4:85992766-85992785	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	85992283	85993095	85992283	ID=MS.gene91655
chr7.4	mRNA	85992283	85993095	85992283	ID=MS.gene91655.t1;Parent=MS.gene91655
chr7.4	exon	85992283	85993095	85992283	ID=MS.gene91655.t1.exon1;Parent=MS.gene91655.t1
chr7.4	CDS	85992283	85993095	85992283	ID=cds.MS.gene91655.t1;Parent=MS.gene91655.t1

Gene Sequence

>MS.gene91655

ATGCCAAAGCTGGACACTAAAAACGTTCCATGGCGGCGGACCTTTGACAAGATCGTATTTGATCATCTTGCACAAGAGATGCAGACCATGAAGTTAGGACATTGGTGGCTTTGCAACACAACTTATAATCTAGAGCATTCAACATTCTCCATCCCACCAAAATTCCTACCAATTGGCCCATTGATGATGGAAAATTACAGCAACAAAAGTTCATTCTGGCAAGAAGACATGACTTCCGTAGATTGGCTAGATAAGCAACCGCCTCAATCTGTCGTATATGTTTCCTTCGATAGTTTGGCAGTAATGGACCAAAACCAATTTAAAGAATTAACATTAGGGCTTGATCTCTTTAACAAGCCTTTTCTTTGGATTGTTCGTCCGAGTAATGACAACAAGGTAAACTATGCATACCCTGATGAGTTCCTTGGAACTAAAGGCAAGATTGTTAGTTGGGCACCGCAGAAAAAGATATTGAACCATCCTGCTATAGCTTGTTTCATTAGTCATTTTGGTTGGAATTCTACTATAGAAGGTGTATATTCTGGTATACCTTTCTTGTGTTGGCCATTTGCAACTGATCAATTTATGAACAAGTCATATATTTGTGATGTGTGGCAGCTTGGATTTGAATTAGACAAGGATGAAAATGGGCTAGTACTAAAGGAAGAGATAAAGAAGAAGGTGGAACAATTGCTTGGAGATGAAGATATAAAAGAAAGATCATTGAAACTAAAGGAGTTAACATTGAATAACATAGAGGAAGATGGTCAATCTTCAAAGAATCTCCAAAACTTTATCAATTGGGCGATGTAA

Protein sequence

>MS.gene91655.t1

MPKLDTKNVPWRRTFDKIVFDHLAQEMQTMKLGHWWLCNTTYNLEHSTFSIPPKFLPIGPLMMENYSNKSSFWQEDMTSVDWLDKQPPQSVVYVSFDSLAVMDQNQFKELTLGLDLFNKPFLWIVRPSNDNKVNYAYPDEFLGTKGKIVSWAPQKKILNHPAIACFISHFGWNSTIEGVYSGIPFLCWPFATDQFMNKSYICDVWQLGFELDKDENGLVLKEEIKKKVEQLLGDEDIKERSLKLKELTLNNIEEDGQSSKNLQNFINWAM