AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92450.t1 MTR_7g023350 98.413 252 4 0 1 252 1 252 3.70e-177 487
MS.gene92450.t1 MTR_1g115405 84.921 252 38 0 1 252 1 252 3.61e-154 429
MS.gene92450.t1 MTR_4g063090 77.381 252 57 0 1 252 1 252 3.12e-132 374
MS.gene92450.t1 MTR_7g103030 69.048 252 76 2 1 252 1 250 2.20e-108 313
MS.gene92450.t1 MTR_1g077540 53.101 258 115 1 1 252 1 258 2.92e-97 285
MS.gene92450.t1 MTR_2g101370 60.324 247 96 2 6 252 5 249 4.77e-95 279
MS.gene92450.t1 MTR_8g013680 59.109 247 99 2 6 252 4 248 1.14e-93 276
MS.gene92450.t1 MTR_6g069040 56.078 255 109 1 1 252 1 255 4.21e-93 275
MS.gene92450.t1 MTR_1g006490 51.822 247 114 3 5 251 3 244 3.27e-81 244
MS.gene92450.t1 MTR_5g012810 54.032 248 111 2 5 252 3 247 5.59e-78 236
MS.gene92450.t1 MTR_1g006590 51.029 243 114 3 5 247 3 240 6.78e-77 233
MS.gene92450.t1 MTR_2g060360 39.749 239 137 2 13 251 11 242 2.35e-57 183
MS.gene92450.t1 MTR_2g094270 39.583 240 120 7 21 240 40 274 5.88e-39 137
MS.gene92450.t1 MTR_7g101190 39.831 236 124 7 13 232 31 264 7.03e-36 129
MS.gene92450.t1 MTR_3g118010 38.288 222 123 5 21 231 37 255 7.00e-35 126
MS.gene92450.t1 MTR_4g059390 36.820 239 127 7 21 240 39 272 1.21e-31 118
MS.gene92450.t1 MTR_8g098375 34.894 235 138 5 10 232 41 272 1.71e-31 117
MS.gene92450.t1 MTR_5g010150 35.443 237 134 6 10 232 41 272 3.32e-31 117
MS.gene92450.t1 MTR_1g095070 42.529 174 88 4 69 231 25 197 6.09e-30 112
MS.gene92450.t1 MTR_5g063930 34.454 238 139 6 17 248 33 259 6.54e-30 112
MS.gene92450.t1 MTR_4g415300 36.404 228 121 8 21 232 56 275 3.76e-29 111
MS.gene92450.t1 MTR_5g082070 34.855 241 130 7 10 232 44 275 5.93e-28 108
MS.gene92450.t1 MTR_3g070210 33.473 239 136 6 10 232 44 275 8.35e-28 108
MS.gene92450.t1 MTR_2g094270 37.864 206 106 6 21 209 40 240 1.40e-27 107
MS.gene92450.t1 MTR_4g084880 34.274 248 151 4 9 251 20 260 8.51e-26 102
MS.gene92450.t1 MTR_3g008920 34.615 234 136 7 20 250 25 244 1.30e-22 93.6
MS.gene92450.t1 MTR_5g079570 32.877 219 133 3 20 235 39 246 1.22e-21 91.3
MS.gene92450.t1 MTR_1g097840 33.036 224 138 7 25 246 78 291 1.66e-21 91.3
MS.gene92450.t1 MTR_2g017620 32.906 234 144 6 20 250 23 246 2.98e-21 89.7
MS.gene92450.t1 MTR_2g017620 32.906 234 144 6 20 250 37 260 4.33e-21 89.7
MS.gene92450.t1 MTR_1g070385 28.959 221 149 4 21 241 62 274 6.57e-21 92.0
MS.gene92450.t1 MTR_2g017590 33.654 208 131 3 20 227 35 235 1.56e-20 88.2
MS.gene92450.t1 MTR_2g017620 34.286 175 111 3 78 251 9 180 5.64e-20 84.7
MS.gene92450.t1 MTR_1g095070 38.365 159 86 4 69 216 25 182 1.91e-19 83.6
MS.gene92450.t1 MTR_2g104940 29.515 227 152 4 25 251 84 302 5.18e-19 84.7
MS.gene92450.t1 MTR_4g006730 29.358 218 146 3 25 242 79 288 5.50e-19 84.7
MS.gene92450.t1 MTR_2g017660 31.364 220 141 4 13 229 28 240 8.74e-19 83.6
MS.gene92450.t1 MTR_8g087710 29.870 231 155 3 20 250 38 261 4.72e-18 81.6
MS.gene92450.t1 MTR_7g010650 31.278 227 142 6 21 245 37 251 4.93e-18 81.6
MS.gene92450.t1 MTR_3g070210 30.088 226 135 6 10 219 44 262 2.36e-17 79.7
MS.gene92450.t1 MTR_2g017610 33.175 211 128 5 20 227 37 237 3.50e-17 79.0
MS.gene92450.t1 MTR_8g087720 29.741 232 156 3 20 251 37 261 3.90e-17 79.0
MS.gene92450.t1 MTR_4g006730 28.708 209 141 3 34 242 1 201 1.09e-16 76.6
MS.gene92450.t1 MTR_2g017570 32.701 211 129 5 20 227 37 237 1.11e-16 77.8
MS.gene92450.t1 MTR_4g006630 29.897 194 128 3 34 227 1 186 1.26e-16 76.6
MS.gene92450.t1 MTR_4g059390 33.918 171 96 5 21 179 39 204 2.26e-16 75.5
MS.gene92450.t1 MTR_5g010150 32.203 177 108 4 10 179 41 212 7.17e-16 74.3
MS.gene92450.t1 MTR_4g075590 27.848 237 146 6 13 229 29 260 1.66e-15 75.1
MS.gene92450.t1 MTR_4g075570 30.055 183 102 6 69 229 96 274 5.32e-15 73.6
MS.gene92450.t1 MTR_8g098375 30.857 175 113 3 10 179 41 212 6.43e-15 72.4
MS.gene92450.t1 MTR_4g415300 33.333 168 95 6 21 179 56 215 1.95e-14 70.9
MS.gene92450.t1 MTR_3g070210 31.210 157 92 4 21 168 55 204 2.11e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92450.t1 AT4G01470 83.333 252 42 0 1 252 1 252 5.49e-153 426
MS.gene92450.t1 AT2G36830 72.222 252 69 1 1 252 1 251 1.56e-127 362
MS.gene92450.t1 AT3G26520 70.356 253 74 1 1 252 1 253 5.09e-122 348
MS.gene92450.t1 AT3G16240 55.061 247 111 0 6 252 4 250 8.20e-93 273
MS.gene92450.t1 AT1G17810 54.688 256 104 2 9 252 12 267 2.07e-89 266
MS.gene92450.t1 AT5G47450 54.251 247 111 1 5 251 3 247 3.63e-89 264
MS.gene92450.t1 AT1G73190 54.086 257 105 2 9 252 12 268 4.20e-89 265
MS.gene92450.t1 AT4G17340 53.659 246 114 0 5 250 3 248 1.66e-80 242
MS.gene92450.t1 AT2G25810 51.004 249 117 3 3 251 1 244 2.11e-76 232
MS.gene92450.t1 AT3G47440 38.889 234 142 1 18 251 20 252 2.33e-51 168
MS.gene92450.t1 AT3G54820 37.179 234 131 6 13 231 30 262 5.24e-36 129
MS.gene92450.t1 AT3G53420 39.823 226 120 6 21 231 39 263 4.91e-35 127
MS.gene92450.t1 AT3G53420 39.823 226 120 6 21 231 39 263 4.91e-35 127
MS.gene92450.t1 AT2G37170 38.496 226 123 6 21 231 37 261 2.09e-34 125
MS.gene92450.t1 AT2G37170 38.496 226 123 6 21 231 80 304 3.97e-34 125
MS.gene92450.t1 AT2G37180 38.053 226 124 6 21 231 37 261 2.05e-33 122
MS.gene92450.t1 AT4G35100 37.838 222 124 5 21 231 38 256 2.31e-33 122
MS.gene92450.t1 AT4G35100 37.838 222 124 5 21 231 38 256 2.31e-33 122
MS.gene92450.t1 AT2G16850 37.387 222 125 5 21 231 36 254 6.43e-33 121
MS.gene92450.t1 AT5G60660 37.611 226 125 6 21 231 39 263 2.42e-32 120
MS.gene92450.t1 AT2G39010 37.118 229 122 6 21 231 38 262 2.23e-31 117
MS.gene92450.t1 AT4G00430 35.417 240 129 7 10 232 42 272 2.68e-29 112
MS.gene92450.t1 AT4G23400 35.556 225 127 6 21 232 53 272 9.70e-29 110
MS.gene92450.t1 AT2G45960 35.983 239 129 7 10 232 41 271 2.13e-28 109
MS.gene92450.t1 AT3G61430 35.965 228 122 7 21 232 52 271 7.77e-28 108
MS.gene92450.t1 AT3G61430 35.965 228 122 7 21 232 52 271 7.77e-28 108
MS.gene92450.t1 AT1G01620 35.371 229 122 7 21 232 52 271 1.19e-27 107
MS.gene92450.t1 AT1G01620 39.130 184 90 6 66 232 21 199 2.36e-26 102
MS.gene92450.t1 AT1G80760 29.958 237 148 5 21 252 80 303 8.32e-21 89.7
MS.gene92450.t1 AT5G37810 31.034 232 149 4 21 250 43 265 1.47e-20 88.6
MS.gene92450.t1 AT5G37820 33.514 185 119 2 68 250 83 265 4.55e-20 87.4
MS.gene92450.t1 AT5G37820 33.514 185 119 2 68 250 119 301 5.11e-20 87.8
MS.gene92450.t1 AT3G06100 29.545 220 148 4 18 237 43 255 2.93e-19 84.7
MS.gene92450.t1 AT2G45960 32.340 235 125 8 10 218 41 267 1.16e-18 83.6
MS.gene92450.t1 AT1G52180 52.703 74 35 0 143 216 51 124 3.12e-18 78.6
MS.gene92450.t1 AT3G06100 30.939 181 123 2 57 237 29 207 4.96e-18 80.5
MS.gene92450.t1 AT2G45960 32.735 223 126 7 10 216 41 255 7.97e-18 80.9
MS.gene92450.t1 AT4G19030 28.452 239 155 5 20 250 54 284 8.56e-18 81.3
MS.gene92450.t1 AT4G10380 29.614 233 154 5 16 246 71 295 1.56e-17 80.5
MS.gene92450.t1 AT4G18910 30.387 181 115 3 78 250 104 281 6.41e-17 78.6
MS.gene92450.t1 AT2G39010 36.025 161 84 6 21 168 38 192 7.30e-16 74.7
MS.gene92450.t1 AT1G31885 31.193 218 130 7 20 227 42 249 1.43e-15 75.5
MS.gene92450.t1 AT1G31885 32.812 192 107 5 48 227 14 195 1.03e-14 72.4
MS.gene92450.t1 AT3G06100 33.083 133 87 2 105 237 44 174 1.06e-13 68.2
MS.gene92450.t1 AT4G00430 33.136 169 94 5 10 168 42 201 2.10e-13 67.8

Find 61 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAATCCAGCTGTTTCTTT+TGG 0.180792 7.3:-76230170 MS.gene92450:CDS
TATTGGGTTGGCCCGTTTAT+TGG 0.192712 7.3:-76230107 MS.gene92450:CDS
ATTCCTATATTTCCTTTCTT+TGG 0.200086 7.3:+76230266 None:intergenic
GGACTCATCATTGGGTCTAT+TGG 0.245534 7.3:-76230124 MS.gene92450:CDS
GAGATCCAATTGCAATTCTA+TGG 0.275529 7.3:+76232163 None:intergenic
GACTCATCATTGGGTCTATT+GGG 0.324641 7.3:-76230123 MS.gene92450:CDS
ATCTTTGATAATATCTTCAT+TGG 0.338371 7.3:-76230065 MS.gene92450:CDS
ATGAGGCCAGCAGGTGTTAC+AGG 0.338897 7.3:+76230970 None:intergenic
GTCACATTTGGTGCCTTCAT+TGG 0.342791 7.3:-76230862 MS.gene92450:CDS
AGGTGTTACAGGGCCATTAT+TGG 0.343459 7.3:+76230981 None:intergenic
GCAATTGCAGCACCAATAAA+CGG 0.354859 7.3:+76230095 None:intergenic
GCAGGCAAACTCACCAATAA+TGG 0.361659 7.3:-76230994 MS.gene92450:intron
CTATTTGTTGCTGTTTCTGT+TGG 0.374459 7.3:-76230919 MS.gene92450:CDS
TATTGGATTGCACAGTTACT+TGG 0.387123 7.3:-76230808 MS.gene92450:CDS
TTGGGCCTGCTGTTGTTAGT+TGG 0.390659 7.3:-76230151 MS.gene92450:CDS
AGAATTGCAATTGGATCTCC+TGG 0.392229 7.3:-76232159 MS.gene92450:CDS
TTTGAAATTGTGATGACATT+CGG 0.394242 7.3:-76230323 MS.gene92450:CDS
GATCCAAAGAAAGGAAATAT+AGG 0.396401 7.3:-76230269 MS.gene92450:CDS
GTTGGACATGGACTCATCAT+TGG 0.396977 7.3:-76230133 MS.gene92450:CDS
AAAAGAAACAGCTGGATTCA+TGG 0.403695 7.3:+76230172 None:intergenic
ATTATTGCTCCAATTGCAAT+TGG 0.414156 7.3:-76230245 MS.gene92450:CDS
TCTGTTGGAGCAAACATCTC+TGG 0.415262 7.3:-76230904 MS.gene92450:CDS
TTAGCTGGTGGAGCATTTGA+TGG 0.419968 7.3:-76230200 MS.gene92450:CDS
CGCTGGTCAAGGCTCTGGCA+TGG 0.424673 7.3:-76232067 MS.gene92450:CDS
GCATCACTATCTCATGCATT+TGG 0.425431 7.3:-76230943 MS.gene92450:CDS
ATTGCAATTGGTGTTATTGT+TGG 0.426769 7.3:-76230233 MS.gene92450:CDS
ACAATAACACCAATTGCAAT+TGG 0.431013 7.3:+76230236 None:intergenic
GCAATCCATAGAATTGCAAT+TGG 0.439495 7.3:-76232168 MS.gene92450:CDS
TATCCTCAGATATATCAGTA+TGG 0.456437 7.3:-76230358 MS.gene92450:CDS
ATTCTTCTCAAGTCTGCTAC+TGG 0.457363 7.3:-76230769 MS.gene92450:CDS
CATCATTGGGTCTATTGGGT+TGG 0.461096 7.3:-76230119 MS.gene92450:CDS
CAATTGCAGCACCAATAAAC+GGG 0.465240 7.3:+76230096 None:intergenic
CTGCTGTTGTTAGTTGGACA+TGG 0.469672 7.3:-76230145 MS.gene92450:CDS
ATTGGATCTCCTGGAGAGGC+TGG 0.474353 7.3:-76232150 MS.gene92450:CDS
TTGGACATGGACTCATCATT+GGG 0.484496 7.3:-76230132 MS.gene92450:CDS
AATATCTTCATTGGTGATGA+TGG 0.487502 7.3:-76230056 MS.gene92450:CDS
GTGAACCCTGCAGTCACATT+TGG 0.490396 7.3:-76230874 MS.gene92450:CDS
ACATTTGGTGCCTTCATTGG+AGG 0.511728 7.3:-76230859 MS.gene92450:CDS
AATGCAGCTCTAATTGCATC+AGG 0.511913 7.3:+76232123 None:intergenic
TCAGGTTGACCAGCCTCTCC+AGG 0.514269 7.3:+76232141 None:intergenic
GTTGGTGCAAATATCTTAGC+TGG 0.517744 7.3:-76230215 MS.gene92450:CDS
CTAGAAATCACTGTTTGAGA+GGG 0.520248 7.3:+76230025 None:intergenic
AAGGCACCAAATGTGACTGC+AGG 0.524218 7.3:+76230868 None:intergenic
TGTAATGTTTCCTCCAATGA+AGG 0.525031 7.3:+76230849 None:intergenic
CAAGTCTGCTACTGGTGGAA+TGG 0.532529 7.3:-76230761 MS.gene92450:intron
GCAGGCCCAAAAGAAACAGC+TGG 0.539419 7.3:+76230164 None:intergenic
AATGGCCCTGTAACACCTGC+TGG 0.541324 7.3:-76230976 MS.gene92450:CDS
CATGTCCAACTAACAACAGC+AGG 0.542428 7.3:+76230146 None:intergenic
GATGCAGCTATGAGGCCAGC+AGG 0.554925 7.3:+76230961 None:intergenic
GAGATAGTGATGCAGCTATG+AGG 0.568245 7.3:+76230953 None:intergenic
ACAGCAGTAGATCCAAAGAA+AGG 0.572893 7.3:-76230278 MS.gene92450:CDS
AATTGTGATGACATTCGGAT+TGG 0.575891 7.3:-76230318 MS.gene92450:CDS
TCTAGAAATCACTGTTTGAG+AGG 0.582825 7.3:+76230024 None:intergenic
CTTCTCAAGTCTGCTACTGG+TGG 0.595244 7.3:-76230766 MS.gene92450:CDS
GTTGGAGCAAACATCTCTGG+TGG 0.599963 7.3:-76230901 MS.gene92450:CDS
TGAGGCCAGCAGGTGTTACA+GGG 0.617532 7.3:+76230971 None:intergenic
TAGAAATCACTGTTTGAGAG+GGG 0.619749 7.3:+76230026 None:intergenic
TGCAATTGGATCTCCTGGAG+AGG 0.626286 7.3:-76232154 MS.gene92450:CDS
AGGCACCAAATGTGACTGCA+GGG 0.650741 7.3:+76230869 None:intergenic
ATTCCATACTGATATATCTG+AGG 0.654480 7.3:+76230355 None:intergenic
GGTGCAAATATCTTAGCTGG+TGG 0.673366 7.3:-76230212 MS.gene92450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTATTAACAAATAAAAT+TGG - chr7.3:76230933-76230952 MS.gene92450:CDS 10.0%
!! TCAATTTCTCAAATTATTAT+CGG - chr7.3:76230336-76230355 MS.gene92450:CDS 15.0%
!!! AAATTTTAGCATAAAAGTTA+TGG + chr7.3:76231093-76231112 None:intergenic 15.0%
!!! ATACTTTCTTGAAATATTTT+CGG + chr7.3:76231557-76231576 None:intergenic 15.0%
!!! TTTTGAGAAGTATTTTATAT+TGG - chr7.3:76231371-76231390 MS.gene92450:intron 15.0%
!! AAAAGAATAAATAGAATGGA+TGG + chr7.3:76230223-76230242 None:intergenic 20.0%
!! AAAGAATAAATAGAATGGAT+GGG + chr7.3:76230222-76230241 None:intergenic 20.0%
!! ACCTAAAAGAATAAATAGAA+TGG + chr7.3:76230227-76230246 None:intergenic 20.0%
!! ATCTTAATAATGCATCAAAA+TGG + chr7.3:76231151-76231170 None:intergenic 20.0%
!! GAAGACATCTATAATAATTT+AGG - chr7.3:76231678-76231697 MS.gene92450:intron 20.0%
!! TAACAAATAAAATTGGAGAA+TGG - chr7.3:76230940-76230959 MS.gene92450:CDS 20.0%
!!! AAAGAAAGTGTAAGAATATA+GGG + chr7.3:76230621-76230640 None:intergenic 20.0%
!!! ATCTTTGATAATATCTTCAT+TGG - chr7.3:76232131-76232150 MS.gene92450:CDS 20.0%
!!! TAAAGAAAGTGTAAGAATAT+AGG + chr7.3:76230622-76230641 None:intergenic 20.0%
!!! TCCATTCTATTTATTCTTTT+AGG - chr7.3:76230223-76230242 MS.gene92450:CDS 20.0%
!!! TTATTTTACTAACACCTTTA+AGG - chr7.3:76230998-76231017 MS.gene92450:intron 20.0%
! AACCATTAAGTAACAAACAA+AGG - chr7.3:76230513-76230532 MS.gene92450:intron 25.0%
! AAGAATAAATAGAATGGATG+GGG + chr7.3:76230221-76230240 None:intergenic 25.0%
! AGGTAATAAGGAAATGTTTA+TGG + chr7.3:76230430-76230449 None:intergenic 25.0%
! ATTACTTATGAACTCTATGA+AGG - chr7.3:76231486-76231505 MS.gene92450:intron 25.0%
! ATTAGACACAAACACAATAT+AGG - chr7.3:76231617-76231636 MS.gene92450:intron 25.0%
! ATTCCTATATTTCCTTTCTT+TGG + chr7.3:76231933-76231952 None:intergenic 25.0%
! CTTACACTTTCTTTAAATGA+AGG - chr7.3:76230627-76230646 MS.gene92450:intron 25.0%
! GAAGTTTATCTTTAAACTAG+TGG + chr7.3:76230797-76230816 None:intergenic 25.0%
! TAATAAGGAAATGTTTATGG+TGG + chr7.3:76230427-76230446 None:intergenic 25.0%
! TCACTGTAAATACTTAATTC+TGG - chr7.3:76230889-76230908 MS.gene92450:CDS 25.0%
!! AAGTGAATTGTTTAGTTTAG+AGG + chr7.3:76230470-76230489 None:intergenic 25.0%
!! TGTGTCAAGTATACTTTTAT+CGG + chr7.3:76231653-76231672 None:intergenic 25.0%
!! TTCCTTTGTTTGTTACTTAA+TGG + chr7.3:76230518-76230537 None:intergenic 25.0%
!! TTTGAAATTGTGATGACATT+CGG - chr7.3:76231873-76231892 MS.gene92450:intron 25.0%
!!! ACTCTTTATTTTCACTTTAG+AGG - chr7.3:76230585-76230604 MS.gene92450:intron 25.0%
!!! ATGACTATTGTTGGTTATTT+TGG - chr7.3:76231180-76231199 MS.gene92450:intron 25.0%
!!! GCTAAAATTTTGAGACTATA+TGG - chr7.3:76231103-76231122 MS.gene92450:intron 25.0%
!!! TTTTGTAATTTGCATATTGC+AGG - chr7.3:76231797-76231816 MS.gene92450:intron 25.0%
ACAATAACACCAATTGCAAT+TGG + chr7.3:76231963-76231982 None:intergenic 30.0%
ATTATTGCTCCAATTGCAAT+TGG - chr7.3:76231951-76231970 MS.gene92450:intron 30.0%
ATTCCATACTGATATATCTG+AGG + chr7.3:76231844-76231863 None:intergenic 30.0%
GAATCGACATTTCAAATTGT+AGG - chr7.3:76230248-76230267 MS.gene92450:CDS 30.0%
GATCCAAAGAAAGGAAATAT+AGG - chr7.3:76231927-76231946 MS.gene92450:intron 30.0%
GCTAGTAAAATGACTATTGT+TGG - chr7.3:76231171-76231190 MS.gene92450:intron 30.0%
GTGTCTAATGTTTGTATCTA+TGG + chr7.3:76231516-76231535 None:intergenic 30.0%
GTTTATCTTTAAACTAGTGG+CGG + chr7.3:76230794-76230813 None:intergenic 30.0%
TATCCTCAGATATATCAGTA+TGG - chr7.3:76231838-76231857 MS.gene92450:intron 30.0%
TTTCCTTATTACCTAAGTTC+CGG - chr7.3:76230436-76230455 MS.gene92450:intron 30.0%
TTTGACCTTGTAACTTTAAG+AGG + chr7.3:76231039-76231058 None:intergenic 30.0%
! ATTGTTTAGTTTAGAGGAAC+CGG + chr7.3:76230464-76230483 None:intergenic 30.0%
! TTGACCTTGTAACTTTAAGA+GGG + chr7.3:76231038-76231057 None:intergenic 30.0%
!! AATATCTTCATTGGTGATGA+TGG - chr7.3:76232140-76232159 MS.gene92450:CDS 30.0%
!! ATTGCAATTGGTGTTATTGT+TGG - chr7.3:76231963-76231982 MS.gene92450:intron 30.0%
AAAAGAAACAGCTGGATTCA+TGG + chr7.3:76232027-76232046 None:intergenic 35.0%
AATTGTGATGACATTCGGAT+TGG - chr7.3:76231878-76231897 MS.gene92450:intron 35.0%
AGCGAAAACAAAAATGAGCA+TGG + chr7.3:76230113-76230132 None:intergenic 35.0%
CAAACAAAGGAAGATATCGT+AGG - chr7.3:76230526-76230545 MS.gene92450:intron 35.0%
CAAGAACATAGATGTTCATG+AGG + chr7.3:76230765-76230784 None:intergenic 35.0%
CATGTGTCAGTATCTTACAA+TGG + chr7.3:76231736-76231755 None:intergenic 35.0%
CTAGAAATCACTGTTTGAGA+GGG + chr7.3:76232174-76232193 None:intergenic 35.0%
GAGATCCAATTGCAATTCTA+TGG + chr7.3:76230036-76230055 None:intergenic 35.0%
GCAATCCATAGAATTGCAAT+TGG - chr7.3:76230028-76230047 MS.gene92450:CDS 35.0%
GGATGGGGAAAATTAAGATA+AGG + chr7.3:76230206-76230225 None:intergenic 35.0%
TAAAAGTTATGGAGTCCGTT+AGG + chr7.3:76231082-76231101 None:intergenic 35.0%
TAGAAATCACTGTTTGAGAG+GGG + chr7.3:76232173-76232192 None:intergenic 35.0%
TATTGGATTGCACAGTTACT+TGG - chr7.3:76231388-76231407 MS.gene92450:intron 35.0%
TGTAATGTTTCCTCCAATGA+AGG + chr7.3:76231350-76231369 None:intergenic 35.0%
!! CTATTTGTTGCTGTTTCTGT+TGG - chr7.3:76231277-76231296 MS.gene92450:intron 35.0%
!!! ATGAATCCAGCTGTTTCTTT+TGG - chr7.3:76232026-76232045 MS.gene92450:intron 35.0%
!!! ATGCTCATTTTTGTTTTCGC+TGG - chr7.3:76230112-76230131 MS.gene92450:CDS 35.0%
!!! ATTTTTGTTTTCGCTGGTCA+AGG - chr7.3:76230118-76230137 MS.gene92450:CDS 35.0%
!!! CTATTGTTGGTTATTTTGGC+AGG - chr7.3:76231184-76231203 MS.gene92450:intron 35.0%
!!! TGAATCCAGCTGTTTCTTTT+GGG - chr7.3:76232027-76232046 MS.gene92450:intron 35.0%
ACAGCAGTAGATCCAAAGAA+AGG - chr7.3:76231918-76231937 MS.gene92450:intron 40.0%
ACCAAAGTTTAAATCCCCTG+AGG + chr7.3:76230700-76230719 None:intergenic 40.0%
AGAATTGCAATTGGATCTCC+TGG - chr7.3:76230037-76230056 MS.gene92450:CDS 40.0%
AGTTTAAATCCCCTGAGGAA+CGG + chr7.3:76230695-76230714 None:intergenic 40.0%
ATTCTTCTCAAGTCTGCTAC+TGG - chr7.3:76231427-76231446 MS.gene92450:intron 40.0%
CAATTGCAGCACCAATAAAC+GGG + chr7.3:76232103-76232122 None:intergenic 40.0%
CGGAGTTCAAATCTCCTTAA+AGG + chr7.3:76231015-76231034 None:intergenic 40.0%
GACTCATCATTGGGTCTATT+GGG - chr7.3:76232073-76232092 MS.gene92450:CDS 40.0%
GATCCGGAACTTAGGTAATA+AGG + chr7.3:76230442-76230461 None:intergenic 40.0%
GCAATTGCAGCACCAATAAA+CGG + chr7.3:76232104-76232123 None:intergenic 40.0%
GCATCACTATCTCATGCATT+TGG - chr7.3:76231253-76231272 MS.gene92450:intron 40.0%
GCTAACACATGTTTGACACT+CGG + chr7.3:76230279-76230298 None:intergenic 40.0%
GTTGGTGCAAATATCTTAGC+TGG - chr7.3:76231981-76232000 MS.gene92450:intron 40.0%
TAGACACGACACTGAAACAT+AGG - chr7.3:76231529-76231548 MS.gene92450:intron 40.0%
TATTACCTAAGTTCCGGATC+CGG - chr7.3:76230442-76230461 MS.gene92450:intron 40.0%
TCCTCAGGGGATTTAAACTT+TGG - chr7.3:76230696-76230715 MS.gene92450:intron 40.0%
TTCATGAAACAGTGACACAG+AGG + chr7.3:76230374-76230393 None:intergenic 40.0%
TTGGACATGGACTCATCATT+GGG - chr7.3:76232064-76232083 MS.gene92450:CDS 40.0%
! AATGCAGCTCTAATTGCATC+AGG + chr7.3:76230076-76230095 None:intergenic 40.0%
! ACCTTGTAACTTTAAGAGGG+CGG + chr7.3:76231035-76231054 None:intergenic 40.0%
! CTGTGTCACTGTTTCATGAA+TGG - chr7.3:76230374-76230393 MS.gene92450:CDS 40.0%
!!! ATTTTAGAACCGTTCCTCAG+GGG - chr7.3:76230683-76230702 MS.gene92450:intron 40.0%
!!! CATTTTAGAACCGTTCCTCA+GGG - chr7.3:76230682-76230701 MS.gene92450:intron 40.0%
!!! TCATTTTAGAACCGTTCCTC+AGG - chr7.3:76230681-76230700 MS.gene92450:intron 40.0%
CATCATTGGGTCTATTGGGT+TGG - chr7.3:76232077-76232096 MS.gene92450:CDS 45.0%
CATGTCCAACTAACAACAGC+AGG + chr7.3:76232053-76232072 None:intergenic 45.0%
CTGCTGTTGTTAGTTGGACA+TGG - chr7.3:76232051-76232070 MS.gene92450:intron 45.0%
GAGATAGTGATGCAGCTATG+AGG + chr7.3:76231246-76231265 None:intergenic 45.0%
GCAGGCAAACTCACCAATAA+TGG - chr7.3:76231202-76231221 MS.gene92450:intron 45.0%
GGACTCATCATTGGGTCTAT+TGG - chr7.3:76232072-76232091 MS.gene92450:CDS 45.0%
GGTGCAAATATCTTAGCTGG+TGG - chr7.3:76231984-76232003 MS.gene92450:intron 45.0%
GTTGGACATGGACTCATCAT+TGG - chr7.3:76232063-76232082 MS.gene92450:CDS 45.0%
TAGTTTAGAGGAACCGGATC+CGG + chr7.3:76230458-76230477 None:intergenic 45.0%
TCCGCCCTCTTAAAGTTACA+AGG - chr7.3:76231031-76231050 MS.gene92450:intron 45.0%
TCTGTTGGAGCAAACATCTC+TGG - chr7.3:76231292-76231311 MS.gene92450:intron 45.0%
! AGGTGTTACAGGGCCATTAT+TGG + chr7.3:76231218-76231237 None:intergenic 45.0%
! TTAGCTGGTGGAGCATTTGA+TGG - chr7.3:76231996-76232015 MS.gene92450:intron 45.0%
! TTCATGAGGTGTTTGCTCAG+TGG + chr7.3:76230751-76230770 None:intergenic 45.0%
!! ACATTTGGTGCCTTCATTGG+AGG - chr7.3:76231337-76231356 MS.gene92450:intron 45.0%
!! GTCACATTTGGTGCCTTCAT+TGG - chr7.3:76231334-76231353 MS.gene92450:intron 45.0%
!! TATTGGGTTGGCCCGTTTAT+TGG - chr7.3:76232089-76232108 MS.gene92450:CDS 45.0%
AGGCACCAAATGTGACTGCA+GGG + chr7.3:76231330-76231349 None:intergenic 50.0%
CAAGTCTGCTACTGGTGGAA+TGG - chr7.3:76231435-76231454 MS.gene92450:intron 50.0%
CTTCTCAAGTCTGCTACTGG+TGG - chr7.3:76231430-76231449 MS.gene92450:intron 50.0%
GTGAACCCTGCAGTCACATT+TGG - chr7.3:76231322-76231341 MS.gene92450:intron 50.0%
GTTGGAGCAAACATCTCTGG+TGG - chr7.3:76231295-76231314 MS.gene92450:intron 50.0%
TGCAATTGGATCTCCTGGAG+AGG - chr7.3:76230042-76230061 MS.gene92450:CDS 50.0%
TTGGGCCTGCTGTTGTTAGT+TGG - chr7.3:76232045-76232064 MS.gene92450:intron 50.0%
! AAGGCACCAAATGTGACTGC+AGG + chr7.3:76231331-76231350 None:intergenic 50.0%
AATGGCCCTGTAACACCTGC+TGG - chr7.3:76231220-76231239 MS.gene92450:intron 55.0%
ACCATTGAGCTCGCGCCTAA+CGG - chr7.3:76231064-76231083 MS.gene92450:intron 55.0%
AGGAACCGGATCCGGAACTT+AGG + chr7.3:76230450-76230469 None:intergenic 55.0%
ATTGGATCTCCTGGAGAGGC+TGG - chr7.3:76230046-76230065 MS.gene92450:CDS 55.0%
GCAGGCCCAAAAGAAACAGC+TGG + chr7.3:76232035-76232054 None:intergenic 55.0%
! ATGAGGCCAGCAGGTGTTAC+AGG + chr7.3:76231229-76231248 None:intergenic 55.0%
! GTTTTCGCTGGTCAAGGCTC+TGG - chr7.3:76230124-76230143 MS.gene92450:CDS 55.0%
! TGAGGCCAGCAGGTGTTACA+GGG + chr7.3:76231228-76231247 None:intergenic 55.0%
GATGCAGCTATGAGGCCAGC+AGG + chr7.3:76231238-76231257 None:intergenic 60.0%
TCCGTTAGGCGCGAGCTCAA+TGG + chr7.3:76231068-76231087 None:intergenic 60.0%
!! TCAGGTTGACCAGCCTCTCC+AGG + chr7.3:76230058-76230077 None:intergenic 60.0%
! CGCTGGTCAAGGCTCTGGCA+TGG - chr7.3:76230129-76230148 MS.gene92450:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 76230025 76232193 76230025 ID=MS.gene92450
chr7.3 mRNA 76230025 76232193 76230025 ID=MS.gene92450.t1;Parent=MS.gene92450
chr7.3 exon 76232061 76232193 76232061 ID=MS.gene92450.t1.exon1;Parent=MS.gene92450.t1
chr7.3 CDS 76232061 76232193 76232061 ID=cds.MS.gene92450.t1;Parent=MS.gene92450.t1
chr7.3 exon 76230762 76231012 76230762 ID=MS.gene92450.t1.exon2;Parent=MS.gene92450.t1
chr7.3 CDS 76230762 76231012 76230762 ID=cds.MS.gene92450.t1;Parent=MS.gene92450.t1
chr7.3 exon 76230025 76230399 76230025 ID=MS.gene92450.t1.exon3;Parent=MS.gene92450.t1
chr7.3 CDS 76230025 76230399 76230025 ID=cds.MS.gene92450.t1;Parent=MS.gene92450.t1
Gene Sequence

>MS.gene92450

ATGGCAATCCATAGAATTGCAATTGGATCTCCTGGAGAGGCTGGTCAACCTGATGCAATTAGAGCTGCATTTGCTGAATTCTTTTCCATGCTCATTTTTGTTTTCGCTGGTCAAGGCTCTGGCATGGCTTACAGTGAGCAAGCACTCTACATATTCACTTAAAACTTGTGTTTTACTACCTTATCTTAATTTTCCCCATCCATTCTATTTATTCTTTTAGGTAGAATCGACATTTCAAATTGTAGGTGTTTCCGAGTGTCAAACATGTGTTAGCGTTCGACGCTAACAGATGTGTTTATATTGAATCACTTTCAATTTCTCAAATTATTATCGGTGTCTATGTCATCCTCTGTGTCACTGTTTCATGAATGGACATGTCATGTTTTTACAATTTTGTTTCCACCATAAACATTTCCTTATTACCTAAGTTCCGGATCCGGTTCCTCTAAACTAAACAATTCACTTTAGAGTGTAAAATGAGTAGTATCAACCATTAAGTAACAAACAAAGGAAGATATCGTAGGCAAGTCATGTCAAATCACGAGTTTGTTAAAATAACAACTCTTTATTTTCACTTTAGAGGATTTAAAAATCCCTATATTCTTACACTTTCTTTAAATGAAGGAAATTGTTATCAATTAAATTGATATTAATTGTCATTTTAGAACCGTTCCTCAGGGGATTTAAACTTTGGTTCTTAATGTTAAAAAATTCAAATGCTTACCACTGAGCAAACACCTCATGAACATCTATGTTCTTGATTTCACCGCCACTAGTTTAAAGATAAACTTCAGTTTTCAGTAATTTGAAGTGTTAAATTTTTTATAAGACAAAGTAGTAAAAATCTTTCACGCATTTAATAGTTCACTGTAAATACTTAATTCTGGCTACATAACATTTTTGTTTTTCTTTTATTAACAAATAAAATTGGAGAATGGAGATTTTATCATTAACTATAATGAGTACTGAATGATTATTTTACTAACACCTTTAAGGAGATTTGAACTCCGCCCTCTTAAAGTTACAAGGTCAAAATTTTACCATTGAGCTCGCGCCTAACGGACTCCATAACTTTTATGCTAAAATTTTGAGACTATATGGTTCAAGAATAAATTTTTTTCATCCATTTTGATGCATTATTAAGATGCTAGTAAAATGACTATTGTTGGTTATTTTGGCAGGCAAACTCACCAATAATGGCCCTGTAACACCTGCTGGCCTCATAGCTGCATCACTATCTCATGCATTTGGACTATTTGTTGCTGTTTCTGTTGGAGCAAACATCTCTGGTGGACATGTGAACCCTGCAGTCACATTTGGTGCCTTCATTGGAGGAAACATTACACTTTTGAGAAGTATTTTATATTGGATTGCACAGTTACTTGGTTCAGTTGTTGCTTGCATTCTTCTCAAGTCTGCTACTGGTGGAATGGTTACTATTAATCTTCAGCTTTAAGCACTATTACTTATGAACTCTATGAAGGAATGCCATAGATACAAACATTAGACACGACACTGAAACATAGGCACCGAAAATATTTCAAGAAAGTATAAGCGATTGAATGTGTCGTGTCGTGTCAAATATATTAGATATTAGACACAAACACAATATAGGCACATAGACGCCGATAAAAGTATACTTGACACATAAACGAAGACATCTATAATAATTTAGGAAAATAAAATTAATTAAATGTAATCACATGCACCATTGTAAGATACTGACACATGTCAGACACTCAAGACATTCTACTAATGATTAAATTGATTTTTTTTGTAATTTGCATATTGCAGGAGACATCAGCATTTGCAATATCCTCAGATATATCAGTATGGAATGCACTTCTTTTTGAAATTGTGATGACATTCGGATTGGTATACACAGTTTATGCAACAGCAGTAGATCCAAAGAAAGGAAATATAGGAATTATTGCTCCAATTGCAATTGGTGTTATTGTTGGTGCAAATATCTTAGCTGGTGGAGCATTTGATGGTGCATCCATGAATCCAGCTGTTTCTTTTGGGCCTGCTGTTGTTAGTTGGACATGGACTCATCATTGGGTCTATTGGGTTGGCCCGTTTATTGGTGCTGCAATTGCTGCTATTATCTTTGATAATATCTTCATTGGTGATGATGGTCATGAACCCCTCTCAAACAGTGATTTCTAG
Protein sequence

>MS.gene92450.t1

MAIHRIAIGSPGEAGQPDAIRAAFAEFFSMLIFVFAGQGSGMAYSKLTNNGPVTPAGLIAASLSHAFGLFVAVSVGANISGGHVNPAVTFGAFIGGNITLLRSILYWIAQLLGSVVACILLKSATGGMETSAFAISSDISVWNALLFEIVMTFGLVYTVYATAVDPKKGNIGIIAPIAIGVIVGANILAGGAFDGASMNPAVSFGPAVVSWTWTHHWVYWVGPFIGAAIAAIIFDNIFIGDDGHEPLSNSDF