AlfalfaGEDB | MS.gene92507

M. sativa cultivar XinJiangDaYe / MS.gene92507

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92507.t1	XP_024636682.1	94.6	295	16	0	1	295	16	310	5.00E-145	523.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92507.t1	O80942	30.6	294	184	6	13	291	10	298	3.0e-21	104.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92507.t1	A0A396I621	94.6	295	16	0	1	295	16	310	3.6e-145	523.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04932	MS.gene92507	0.802456	5.56E-49	-1.69E-46
MS.gene049549	MS.gene92507	0.832629	8.48E-56	-1.69E-46
MS.gene051131	MS.gene92507	0.807885	4.06E-50	-1.69E-46
MS.gene052211	MS.gene92507	0.859119	5.16E-63	-1.69E-46
MS.gene052212	MS.gene92507	0.802104	6.57E-49	-1.69E-46
MS.gene05240	MS.gene92507	0.80063	1.32E-48	-1.69E-46
MS.gene05244	MS.gene92507	0.800409	1.46E-48	-1.69E-46
MS.gene052606	MS.gene92507	0.832753	7.90E-56	-1.69E-46
MS.gene053403	MS.gene92507	0.806937	6.45E-50	-1.69E-46
MS.gene053406	MS.gene92507	0.82981	4.18E-55	-1.69E-46
MS.gene053887	MS.gene92507	0.803027	4.24E-49	-1.69E-46
MS.gene054146	MS.gene92507	0.835024	2.13E-56	-1.69E-46
MS.gene054742	MS.gene92507	0.802534	5.36E-49	-1.69E-46
MS.gene055240	MS.gene92507	0.854345	1.31E-61	-1.69E-46
MS.gene056590	MS.gene92507	0.820402	6.99E-53	-1.69E-46
MS.gene056591	MS.gene92507	0.81911	1.38E-52	-1.69E-46
MS.gene056748	MS.gene92507	0.818893	1.55E-52	-1.69E-46
MS.gene058603	MS.gene92507	0.81655	5.22E-52	-1.69E-46
MS.gene059955	MS.gene92507	0.820927	5.30E-53	-1.69E-46
MS.gene060087	MS.gene92507	0.810581	1.07E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92507.t1	MTR_4g021270	96.259	294	11	0	1	294	16	309	0.0	591
MS.gene92507.t1	MTR_3g068095	34.495	287	106	11	21	290	33	254	1.87e-32	120
MS.gene92507.t1	MTR_5g080660	55.789	95	35	2	20	107	23	117	1.70e-28	109
MS.gene92507.t1	MTR_5g014400	40.113	177	68	9	20	190	58	202	4.62e-26	103
MS.gene92507.t1	MTR_2g076060	79.070	43	8	1	36	77	41	83	3.50e-19	84.7
MS.gene92507.t1	MTR_1g070265	82.051	39	7	0	38	76	29	67	3.88e-17	78.2
MS.gene92507.t1	MTR_4g064873	70.000	40	12	0	43	82	20	59	5.70e-14	68.9
MS.gene92507.t1	MTR_7g063220	66.667	42	14	0	44	85	24	65	7.27e-14	67.8
MS.gene92507.t1	MTR_1g075430	69.444	36	11	0	44	79	18	53	5.79e-12	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92507.t1	AT2G37740	70.270	74	16	2	13	86	10	77	6.66e-27	107
MS.gene92507.t1	AT5G06070	67.606	71	21	1	10	78	17	87	1.44e-25	101
MS.gene92507.t1	AT4G17810	70.370	54	15	1	20	73	21	73	1.54e-21	89.7
MS.gene92507.t1	AT4G17810	70.370	54	15	1	20	73	21	73	1.77e-21	90.5
MS.gene92507.t1	AT2G42410	67.273	55	15	1	37	91	40	91	2.03e-19	85.1
MS.gene92507.t1	AT2G42410	67.273	55	15	1	37	91	40	91	2.03e-19	85.1
MS.gene92507.t1	AT2G42410	67.273	55	15	1	37	91	66	117	2.40e-19	85.5
MS.gene92507.t1	AT3G23130	81.395	43	8	0	36	78	37	79	1.37e-18	82.4
MS.gene92507.t1	AT3G09290	71.429	35	10	0	44	78	33	67	9.94e-12	62.8

Find 57 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAGTTGAAAGGAGAAATT+AGG	0.144993	4.2:-81340913	None:intergenic
TCTACCTCCTCAATTGAATT+AGG	0.148497	4.2:-81341265	None:intergenic
AAACCACAAGTAGGAGAATT+AGG	0.187380	4.2:-81340791	None:intergenic
AAATGATCAAGATGAGAATT+AGG	0.200848	4.2:-81340833	None:intergenic
ATCACCTTCGTCGGCTTCTA+AGG	0.266590	4.2:+81340871	MS.gene92507:CDS
AACCCTTCTTCTTCTTGTTA+TGG	0.304635	4.2:+81340470	MS.gene92507:CDS
CCAAAGGTATAATAGATTCT+AGG	0.312053	4.2:+81341023	MS.gene92507:CDS
GATGCAGCTGCTGGGTCACT+TGG	0.326814	4.2:+81340524	MS.gene92507:CDS
AGAGTTGAAAGGAGAAATTA+GGG	0.331194	4.2:-81340912	None:intergenic
TAATTCTCCTACTTGTGGTT+TGG	0.335006	4.2:+81340793	MS.gene92507:CDS
CTTCTTGTTATGGTGATTCA+TGG	0.338824	4.2:+81340480	MS.gene92507:CDS
CTACCTCCTCAATTGAATTA+GGG	0.346783	4.2:-81341264	None:intergenic
TTTGCTGAAGATGCAGCTGC+TGG	0.353393	4.2:+81340515	MS.gene92507:CDS
TGTAGCTATGAGCTTGAATT+TGG	0.353912	4.2:+81341081	MS.gene92507:CDS
TTTAGGTCAGCACAGGCATT+AGG	0.354033	4.2:+81340599	MS.gene92507:CDS
CCTAGAATCTATTATACCTT+TGG	0.378017	4.2:-81341023	None:intergenic
TCGGCTTCTAAGGCTTTATT+CGG	0.401138	4.2:+81340881	MS.gene92507:CDS
CTTTCAACTCTTCTGAATCA+TGG	0.401376	4.2:+81340924	MS.gene92507:CDS
TTCAATTGAGGAGGTAGATC+TGG	0.403141	4.2:+81341270	MS.gene92507:CDS
GAACATAATATATCATTTCC+AGG	0.405382	4.2:-81340956	None:intergenic
AATGATCAAGATGAGAATTA+GGG	0.405455	4.2:-81340832	None:intergenic
GATTCTAGGTTTCGAGGTAA+AGG	0.408364	4.2:+81341037	MS.gene92507:CDS
ATTCTAGGTTTCGAGGTAAA+GGG	0.409155	4.2:+81341038	MS.gene92507:CDS
AGTTAGTGAACAAGCAAGAT+TGG	0.412450	4.2:-81340447	None:intergenic
AACCACAAGTAGGAGAATTA+GGG	0.432833	4.2:-81340790	None:intergenic
AAGTAGGAGAATTAGGGAAT+AGG	0.457191	4.2:-81340784	None:intergenic
AAGCTACAAGAATATGATCT+TGG	0.459400	4.2:-81340563	None:intergenic
TTCCCTAATTCTCCTACTTG+TGG	0.462438	4.2:+81340788	MS.gene92507:CDS
AGGAGGTAGATCTGGAGTTA+AGG	0.476987	4.2:+81341278	MS.gene92507:CDS
ATGCAGCTGCTGGGTCACTT+GGG	0.477889	4.2:+81340525	MS.gene92507:CDS
GTTCAACCCTAATTCAATTG+AGG	0.483460	4.2:+81341258	MS.gene92507:CDS
TTCTTGTTATGGTGATTCAT+GGG	0.486889	4.2:+81340481	MS.gene92507:CDS
TTGCTGAAGATGCAGCTGCT+GGG	0.487756	4.2:+81340516	MS.gene92507:CDS
AAGATTTCAATTGCAAGAAG+AGG	0.493658	4.2:+81341152	MS.gene92507:CDS
CGATTTCAAGCTCGTGATGA+TGG	0.497652	4.2:-81340712	None:intergenic
GAATCATGGCTGAATTTACC+TGG	0.500114	4.2:+81340938	MS.gene92507:CDS
TTCAACTTCATCACCTTCGT+CGG	0.511632	4.2:+81340862	MS.gene92507:CDS
TGCAGCTGCTGGGTCACTTG+GGG	0.517099	4.2:+81340526	MS.gene92507:CDS
TCACCATAACAAGAAGAAGA+AGG	0.520396	4.2:-81340473	None:intergenic
TATGCTACTTAACTATACCT+TGG	0.528071	4.2:-81341314	None:intergenic
GCAGCTGCTGGGTCACTTGG+GGG	0.531991	4.2:+81340527	MS.gene92507:CDS
CTACAAGAATATGATCTTGG+TGG	0.544295	4.2:-81340560	None:intergenic
AAGAGAGTTTAGGTCAGCAC+AGG	0.547028	4.2:+81340592	MS.gene92507:CDS
TGGAGTTAAGGCTTGGTACA+AGG	0.549906	4.2:+81341290	MS.gene92507:CDS
CATGATTCAGAAGAGTTGAA+AGG	0.568556	4.2:-81340923	None:intergenic
GTAGATCTGGAGTTAAGGCT+TGG	0.574970	4.2:+81341283	MS.gene92507:CDS
TTTGATCATAAAGTCTCCAA+AGG	0.581337	4.2:+81341007	MS.gene92507:CDS
ATAATAGATTCTAGGTTTCG+AGG	0.582395	4.2:+81341031	MS.gene92507:CDS
CACCATAACAAGAAGAAGAA+GGG	0.582469	4.2:-81340472	None:intergenic
TTCTAGGTTTCGAGGTAAAG+GGG	0.591482	4.2:+81341039	MS.gene92507:CDS
CAACCCTAATTCAATTGAGG+AGG	0.592265	4.2:+81341261	MS.gene92507:CDS
TTATAAACCAAACCACAAGT+AGG	0.601660	4.2:-81340800	None:intergenic
AGGTCAGCACAGGCATTAGG+TGG	0.610335	4.2:+81340602	MS.gene92507:CDS
TCTAGGTTTCGAGGTAAAGG+GGG	0.621509	4.2:+81341040	MS.gene92507:CDS
AAGGCTTGGTACAAGGTCCA+AGG	0.631211	4.2:+81341297	MS.gene92507:CDS
GTACTCTCAAATTCAACACG+TGG	0.655276	4.2:-81341121	None:intergenic
AAAGCCTTAGAAGCCGACGA+AGG	0.711061	4.2:-81340875	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CATATTTTGTTGTTTATCAA+AGG	-	chr4.2:81341221-81341240	None:intergenic	20.0%
!!!	TAAAGAATCTGATTATTTTG+TGG	-	chr4.2:81340674-81340693	None:intergenic	20.0%
!!!	TTATAAACATTTTGCATAAG+TGG	-	chr4.2:81340758-81340777	None:intergenic	20.0%
!	AAATGATCAAGATGAGAATT+AGG	-	chr4.2:81340836-81340855	None:intergenic	25.0%
!	AATGATCAAGATGAGAATTA+GGG	-	chr4.2:81340835-81340854	None:intergenic	25.0%
!	GAACATAATATATCATTTCC+AGG	-	chr4.2:81340959-81340978	None:intergenic	25.0%
	AAGAGTTGAAAGGAGAAATT+AGG	-	chr4.2:81340916-81340935	None:intergenic	30.0%
	AAGATTTCAATTGCAAGAAG+AGG	+	chr4.2:81341152-81341171	MS.gene92507:CDS	30.0%
	AAGCTACAAGAATATGATCT+TGG	-	chr4.2:81340566-81340585	None:intergenic	30.0%
	AGAGTTGAAAGGAGAAATTA+GGG	-	chr4.2:81340915-81340934	None:intergenic	30.0%
	ATAATAGATTCTAGGTTTCG+AGG	+	chr4.2:81341031-81341050	MS.gene92507:CDS	30.0%
	CCTAGAATCTATTATACCTT+TGG	-	chr4.2:81341026-81341045	None:intergenic	30.0%
	TTATAAACCAAACCACAAGT+AGG	-	chr4.2:81340803-81340822	None:intergenic	30.0%
	TTTGATCATAAAGTCTCCAA+AGG	+	chr4.2:81341007-81341026	MS.gene92507:CDS	30.0%
!	CCAAAGGTATAATAGATTCT+AGG	+	chr4.2:81341023-81341042	MS.gene92507:CDS	30.0%
!	TTCTTGTTATGGTGATTCAT+GGG	+	chr4.2:81340481-81340500	MS.gene92507:CDS	30.0%
	AAACCACAAGTAGGAGAATT+AGG	-	chr4.2:81340794-81340813	None:intergenic	35.0%
	AACCACAAGTAGGAGAATTA+GGG	-	chr4.2:81340793-81340812	None:intergenic	35.0%
	AACCCTTCTTCTTCTTGTTA+TGG	+	chr4.2:81340470-81340489	MS.gene92507:CDS	35.0%
	AAGTAGGAGAATTAGGGAAT+AGG	-	chr4.2:81340787-81340806	None:intergenic	35.0%
	AGTTAGTGAACAAGCAAGAT+TGG	-	chr4.2:81340450-81340469	None:intergenic	35.0%
	ATTCTAGGTTTCGAGGTAAA+GGG	+	chr4.2:81341038-81341057	MS.gene92507:CDS	35.0%
	CACCATAACAAGAAGAAGAA+GGG	-	chr4.2:81340475-81340494	None:intergenic	35.0%
	CATGATTCAGAAGAGTTGAA+AGG	-	chr4.2:81340926-81340945	None:intergenic	35.0%
	CTACAAGAATATGATCTTGG+TGG	-	chr4.2:81340563-81340582	None:intergenic	35.0%
	CTACCTCCTCAATTGAATTA+GGG	-	chr4.2:81341267-81341286	None:intergenic	35.0%
	CTTTCAACTCTTCTGAATCA+TGG	+	chr4.2:81340924-81340943	MS.gene92507:CDS	35.0%
	GTTCAACCCTAATTCAATTG+AGG	+	chr4.2:81341258-81341277	MS.gene92507:CDS	35.0%
	TAATTCTCCTACTTGTGGTT+TGG	+	chr4.2:81340793-81340812	MS.gene92507:CDS	35.0%
	TCACCATAACAAGAAGAAGA+AGG	-	chr4.2:81340476-81340495	None:intergenic	35.0%
	TCTACCTCCTCAATTGAATT+AGG	-	chr4.2:81341268-81341287	None:intergenic	35.0%
	TGTAGCTATGAGCTTGAATT+TGG	+	chr4.2:81341081-81341100	MS.gene92507:CDS	35.0%
!	CTTCTTGTTATGGTGATTCA+TGG	+	chr4.2:81340480-81340499	MS.gene92507:CDS	35.0%
!	GCTTTTGTAGAAGAGAGTTT+AGG	+	chr4.2:81340582-81340601	MS.gene92507:CDS	35.0%
	CAACCCTAATTCAATTGAGG+AGG	+	chr4.2:81341261-81341280	MS.gene92507:CDS	40.0%
	GAATCATGGCTGAATTTACC+TGG	+	chr4.2:81340938-81340957	MS.gene92507:CDS	40.0%
	GATTCTAGGTTTCGAGGTAA+AGG	+	chr4.2:81341037-81341056	MS.gene92507:CDS	40.0%
	GTACTCTCAAATTCAACACG+TGG	-	chr4.2:81341124-81341143	None:intergenic	40.0%
	TTCAACTTCATCACCTTCGT+CGG	+	chr4.2:81340862-81340881	MS.gene92507:CDS	40.0%
	TTCAATTGAGGAGGTAGATC+TGG	+	chr4.2:81341270-81341289	MS.gene92507:CDS	40.0%
	TTCCCTAATTCTCCTACTTG+TGG	+	chr4.2:81340788-81340807	MS.gene92507:CDS	40.0%
	TTCTAGGTTTCGAGGTAAAG+GGG	+	chr4.2:81341039-81341058	MS.gene92507:CDS	40.0%
!!	TCGGCTTCTAAGGCTTTATT+CGG	+	chr4.2:81340881-81340900	MS.gene92507:CDS	40.0%
	AAGAGAGTTTAGGTCAGCAC+AGG	+	chr4.2:81340592-81340611	MS.gene92507:CDS	45.0%
	CGATTTCAAGCTCGTGATGA+TGG	-	chr4.2:81340715-81340734	None:intergenic	45.0%
	TCTAGGTTTCGAGGTAAAGG+GGG	+	chr4.2:81341040-81341059	MS.gene92507:CDS	45.0%
	TTTAGGTCAGCACAGGCATT+AGG	+	chr4.2:81340599-81340618	MS.gene92507:CDS	45.0%
!	AGGAGGTAGATCTGGAGTTA+AGG	+	chr4.2:81341278-81341297	MS.gene92507:CDS	45.0%
!	GTAGATCTGGAGTTAAGGCT+TGG	+	chr4.2:81341283-81341302	MS.gene92507:CDS	45.0%
!	TGGAGTTAAGGCTTGGTACA+AGG	+	chr4.2:81341290-81341309	MS.gene92507:CDS	45.0%
	AAAGCCTTAGAAGCCGACGA+AGG	-	chr4.2:81340878-81340897	None:intergenic	50.0%
	GGTCACTTGGGGGATGTATA+TGG	+	chr4.2:81340537-81340556	MS.gene92507:CDS	50.0%
	TTGCTGAAGATGCAGCTGCT+GGG	+	chr4.2:81340516-81340535	MS.gene92507:CDS	50.0%
	TTTGCTGAAGATGCAGCTGC+TGG	+	chr4.2:81340515-81340534	MS.gene92507:CDS	50.0%
!	AAGGCTTGGTACAAGGTCCA+AGG	+	chr4.2:81341297-81341316	MS.gene92507:CDS	50.0%
!!	ATCACCTTCGTCGGCTTCTA+AGG	+	chr4.2:81340871-81340890	MS.gene92507:CDS	50.0%
	AGGTCAGCACAGGCATTAGG+TGG	+	chr4.2:81340602-81340621	MS.gene92507:CDS	55.0%
	ATGCAGCTGCTGGGTCACTT+GGG	+	chr4.2:81340525-81340544	MS.gene92507:CDS	55.0%
	GATGCAGCTGCTGGGTCACT+TGG	+	chr4.2:81340524-81340543	MS.gene92507:CDS	60.0%
	TGCAGCTGCTGGGTCACTTG+GGG	+	chr4.2:81340526-81340545	MS.gene92507:CDS	60.0%
	GCAGCTGCTGGGTCACTTGG+GGG	+	chr4.2:81340527-81340546	MS.gene92507:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	81340437	81341324	81340437	ID=MS.gene92507
chr4.2	mRNA	81340437	81341324	81340437	ID=MS.gene92507.t1;Parent=MS.gene92507
chr4.2	exon	81340437	81341324	81340437	ID=MS.gene92507.t1.exon1;Parent=MS.gene92507.t1
chr4.2	CDS	81340437	81341324	81340437	ID=cds.MS.gene92507.t1;Parent=MS.gene92507.t1

Gene Sequence

>MS.gene92507

ATGAGTAATTCCAATCTTGCTTGTTCACTAACTAACCCTTCTTCTTCTTGTTATGGTGATTCATGGGAAGAACAAGCTTTTGCTGAAGATGCAGCTGCTGGGTCACTTGGGGGATGTATATGGCCACCAAGATCATATTCTTGTAGCTTTTGTAGAAGAGAGTTTAGGTCAGCACAGGCATTAGGTGGACATATGAATGTTCATAGAAAAGATAGAGCAAGACTCAAACAACAACCACAAAATAATCAGATTCTTTATCATAATCATCATCATCACCATCATCACGAGCTTGAAATCGCAACTCATCATGTTCAAAAGCCACTTATGCAAAATGTTTATAATAATAACCTATTCCCTAATTCTCCTACTTGTGGTTTGGTTTATAATAAAACTAACCCTAATTCTCATCTTGATCATTTTGTATCTTCAACTTCATCACCTTCGTCGGCTTCTAAGGCTTTATTCGGAGATGAAACCCTAATTTCTCCTTTCAACTCTTCTGAATCATGGCTGAATTTACCTGGAAATGATATATTATGTTCAACAAAGTTTCAACTTGCTGTTGATAATTTTGATCATAAAGTCTCCAAAGGTATAATAGATTCTAGGTTTCGAGGTAAAGGGGGAAACGACAACGAAAGTGATGTAGCTATGAGCTTGAATTTGGTTTTGTGTAGAACTCATCCACGTGTTGAATTTGAGAGTACTAAAGAAGAAGATTTCAATTGCAAGAAGAGGAAAAAAGATTCTTCATCAAATCAATTATTCTCTAACTCAAGCACCTTTGATAAACAACAAAATATGCAAGCAAAGATGTTTGAGTTCAACCCTAATTCAATTGAGGAGGTAGATCTGGAGTTAAGGCTTGGTACAAGGTCCAAGGTATAG

Protein sequence

>MS.gene92507.t1

MSNSNLACSLTNPSSSCYGDSWEEQAFAEDAAAGSLGGCIWPPRSYSCSFCRREFRSAQALGGHMNVHRKDRARLKQQPQNNQILYHNHHHHHHHELEIATHHVQKPLMQNVYNNNLFPNSPTCGLVYNKTNPNSHLDHFVSSTSSPSSASKALFGDETLISPFNSSESWLNLPGNDILCSTKFQLAVDNFDHKVSKGIIDSRFRGKGGNDNESDVAMSLNLVLCRTHPRVEFESTKEEDFNCKKRKKDSSSNQLFSNSSTFDKQQNMQAKMFEFNPNSIEEVDLELRLGTRSKV