AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94488

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94488.t1 MTR_2g073500 94.556 349 18 1 1 349 1 348 0.0 688
MS.gene94488.t1 MTR_1g111010 64.516 341 109 5 12 347 9 342 2.10e-164 463
MS.gene94488.t1 MTR_2g073510 52.121 330 140 5 28 346 31 353 5.09e-124 360
MS.gene94488.t1 MTR_3g049330 50.456 329 144 6 30 347 31 351 1.13e-119 349
MS.gene94488.t1 MTR_4g088030 46.389 360 171 9 1 347 1 351 1.74e-110 326
MS.gene94488.t1 MTR_2g015720 48.624 327 148 7 30 346 42 358 5.06e-110 325
MS.gene94488.t1 MTR_3g035830 47.590 332 149 8 28 346 29 348 2.95e-109 323
MS.gene94488.t1 MTR_5g034810 47.130 331 154 8 30 349 35 355 4.46e-109 323
MS.gene94488.t1 MTR_4g087730 49.085 328 148 7 30 347 45 363 1.54e-108 322
MS.gene94488.t1 MTR_4g079700 47.748 333 154 6 24 346 41 363 1.84e-107 319
MS.gene94488.t1 MTR_3g021060 46.108 334 155 8 28 348 44 365 3.66e-106 315
MS.gene94488.t1 MTR_3g021090 47.416 329 146 9 28 342 29 344 1.69e-104 313
MS.gene94488.t1 MTR_2g091175 45.897 329 156 8 30 347 50 367 9.68e-103 307
MS.gene94488.t1 MTR_5g071350 42.541 362 165 9 20 347 48 400 2.14e-94 287
MS.gene94488.t1 MTR_3g464570 41.329 346 170 8 27 347 40 377 1.13e-91 279
MS.gene94488.t1 MTR_1g050448 43.155 336 160 9 30 347 53 375 4.22e-89 272
MS.gene94488.t1 MTR_1g112210 43.155 336 164 9 28 346 57 382 5.22e-89 272
MS.gene94488.t1 MTR_1g112210 43.155 336 151 10 28 346 57 369 2.53e-87 268
MS.gene94488.t1 MTR_0136s0010 39.769 347 174 9 30 347 170 510 5.83e-82 259
MS.gene94488.t1 MTR_7g093450 39.769 347 174 10 30 347 248 588 6.24e-82 261
MS.gene94488.t1 MTR_4g121560 40.058 347 173 13 30 347 114 454 2.39e-79 250
MS.gene94488.t1 MTR_5g059400 38.136 354 179 10 26 347 171 516 2.52e-77 247
MS.gene94488.t1 MTR_1g116130 38.136 354 179 10 26 347 171 516 2.52e-77 247
MS.gene94488.t1 MTR_7g109550 39.474 342 171 12 30 346 137 467 1.91e-76 243
MS.gene94488.t1 MTR_3g090120 38.398 362 191 11 10 348 109 461 3.26e-75 239
MS.gene94488.t1 MTR_4g126950 36.494 348 186 12 28 347 107 447 1.65e-74 237
MS.gene94488.t1 MTR_5g033800 35.328 351 193 10 26 348 141 485 8.76e-74 236
MS.gene94488.t1 MTR_4g016940 35.429 350 189 9 28 346 84 427 7.68e-72 229
MS.gene94488.t1 MTR_1g058380 37.356 348 185 11 28 347 53 395 2.15e-70 225
MS.gene94488.t1 MTR_8g069790 34.943 352 195 10 25 348 173 518 2.66e-70 228
MS.gene94488.t1 MTR_3g464570 40.441 272 135 6 101 347 4 273 5.25e-68 215
MS.gene94488.t1 MTR_7g094750 36.415 357 176 12 30 348 91 434 1.15e-67 219
MS.gene94488.t1 MTR_3g070900 34.746 354 192 10 27 347 103 450 2.10e-67 219
MS.gene94488.t1 MTR_8g093530 36.232 345 190 10 25 349 85 419 1.51e-65 213
MS.gene94488.t1 MTR_3g071650 35.795 352 183 11 30 347 89 431 1.54e-65 213
MS.gene94488.t1 MTR_8g463790 32.493 357 197 10 26 349 67 412 1.91e-64 210
MS.gene94488.t1 MTR_4g014440 31.738 397 214 12 1 347 19 408 2.11e-64 210
MS.gene94488.t1 MTR_4g014430 34.670 349 190 10 28 346 106 446 1.16e-63 209
MS.gene94488.t1 MTR_2g022560 37.429 350 181 11 30 348 97 439 4.78e-63 207
MS.gene94488.t1 MTR_7g068920 33.807 352 192 10 28 346 78 421 5.07e-62 204
MS.gene94488.t1 MTR_5g083260 35.112 356 186 13 27 347 80 425 1.40e-60 200
MS.gene94488.t1 MTR_7g070060 31.921 354 198 9 30 347 64 410 2.86e-59 196
MS.gene94488.t1 MTR_5g082880 32.768 354 196 11 30 347 165 512 2.06e-57 194
MS.gene94488.t1 MTR_5g033800 34.836 244 143 6 26 259 141 378 1.50e-49 170
MS.gene94488.t1 MTR_7g094750 35.341 249 129 7 30 259 91 326 1.23e-44 156
MS.gene94488.t1 MTR_7g070060 29.773 309 178 8 76 347 2 308 1.26e-43 153
MS.gene94488.t1 MTR_7g070060 31.224 237 151 5 30 259 64 295 5.19e-39 140
MS.gene94488.t1 MTR_5g069320 34.686 271 133 10 82 339 147 386 2.34e-38 141
MS.gene94488.t1 MTR_5g035150 29.609 358 206 14 27 346 82 431 4.43e-34 130
MS.gene94488.t1 MTR_6g061710 28.418 373 198 14 24 346 161 514 3.95e-33 129
MS.gene94488.t1 MTR_4g092830 26.050 357 216 14 30 348 48 394 1.08e-32 126
MS.gene94488.t1 MTR_8g093870 28.080 349 216 11 27 346 130 472 1.11e-31 124
MS.gene94488.t1 MTR_1g076690 27.778 378 206 15 20 342 201 566 1.28e-30 122
MS.gene94488.t1 MTR_5g035090 26.891 357 215 12 27 346 80 427 1.08e-29 118
MS.gene94488.t1 MTR_2g016060 28.571 343 193 15 49 348 69 402 4.18e-28 113
MS.gene94488.t1 MTR_6g016880 28.650 363 208 13 22 346 70 419 4.24e-28 114
MS.gene94488.t1 MTR_5g035080 27.049 366 204 15 27 346 72 420 4.54e-28 114
MS.gene94488.t1 MTR_4g125060 27.874 348 216 10 28 346 130 471 5.49e-28 114
MS.gene94488.t1 MTR_6g016240 26.685 356 212 14 30 346 71 416 1.00e-27 112
MS.gene94488.t1 MTR_4g125060 27.874 348 216 10 28 346 198 539 1.29e-27 113
MS.gene94488.t1 MTR_4g125060 27.874 348 216 10 28 346 169 510 1.32e-27 113
MS.gene94488.t1 MTR_6g007777 25.348 359 223 12 23 346 109 457 2.06e-27 112
MS.gene94488.t1 MTR_8g104970 26.849 365 206 14 27 346 117 465 1.03e-26 110
MS.gene94488.t1 MTR_3g109840 28.212 358 193 16 30 342 73 411 1.82e-26 109
MS.gene94488.t1 MTR_7g033230 27.147 361 212 11 26 346 260 609 2.07e-26 110
MS.gene94488.t1 MTR_7g019120 27.019 359 219 10 22 346 71 420 3.77e-26 108
MS.gene94488.t1 MTR_7g033230 28.142 366 202 13 26 346 330 679 5.71e-26 109
MS.gene94488.t1 MTR_5g035070 28.451 355 208 14 30 346 87 433 1.46e-25 107
MS.gene94488.t1 MTR_8g027900 25.905 359 218 12 28 348 209 557 8.19e-25 105
MS.gene94488.t1 MTR_6g016875 26.629 353 215 13 30 346 64 408 1.22e-24 103
MS.gene94488.t1 MTR_3g021150 44.444 108 45 3 222 316 1 106 1.12e-23 94.7
MS.gene94488.t1 MTR_8g099300 25.000 356 220 10 30 347 74 420 3.29e-23 100
MS.gene94488.t1 MTR_5g035120 41.837 98 51 2 27 124 72 163 7.73e-19 83.2
MS.gene94488.t1 MTR_7g019140 38.835 103 57 1 22 124 71 167 1.32e-17 79.7
MS.gene94488.t1 MTR_2g022580 32.500 120 67 4 96 202 2 120 6.49e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94488.t1 AT1G78710 55.927 329 127 6 30 348 38 358 3.56e-136 392
MS.gene94488.t1 AT2G30900 50.904 332 143 7 30 348 43 367 2.80e-117 344
MS.gene94488.t1 AT1G29050 49.394 330 142 9 30 346 61 378 3.62e-112 332
MS.gene94488.t1 AT3G14850 50.149 335 143 8 28 348 32 356 3.87e-110 325
MS.gene94488.t1 AT2G42570 48.943 331 146 8 30 348 47 366 1.30e-108 322
MS.gene94488.t1 AT2G31110 48.024 329 148 8 30 346 45 362 4.91e-107 318
MS.gene94488.t1 AT2G34070 47.416 329 151 9 30 346 65 383 7.95e-105 313
MS.gene94488.t1 AT1G78710 48.758 322 108 8 30 348 31 298 3.82e-101 300
MS.gene94488.t1 AT2G30900 55.310 226 93 4 30 254 43 261 9.88e-86 260
MS.gene94488.t1 AT5G58600 42.319 345 163 10 30 347 66 401 1.50e-85 264
MS.gene94488.t1 AT3G54260 40.774 336 174 8 30 349 51 377 1.67e-85 263
MS.gene94488.t1 AT5G06700 39.193 347 176 10 30 347 255 595 8.18e-82 260
MS.gene94488.t1 AT3G14850 49.416 257 112 6 106 348 1 253 6.39e-81 247
MS.gene94488.t1 AT2G30010 40.171 351 168 10 30 347 56 397 6.65e-81 252
MS.gene94488.t1 AT2G30010 40.171 351 168 10 30 347 99 440 9.53e-81 253
MS.gene94488.t1 AT3G12060 38.395 349 180 9 28 347 194 536 4.94e-80 254
MS.gene94488.t1 AT3G62390 38.484 343 182 9 28 347 136 472 1.53e-79 251
MS.gene94488.t1 AT5G49340 38.095 378 182 13 14 347 73 442 1.70e-76 243
MS.gene94488.t1 AT2G34070 47.036 253 118 7 106 346 1 249 5.02e-76 234
MS.gene94488.t1 AT2G31110 48.889 225 107 4 30 254 45 261 1.38e-75 234
MS.gene94488.t1 AT1G60790 37.607 351 185 10 28 348 188 534 3.08e-73 236
MS.gene94488.t1 AT5G20590 36.232 345 188 11 30 347 141 480 2.84e-72 233
MS.gene94488.t1 AT1G48880 39.210 329 176 10 30 342 115 435 3.20e-72 231
MS.gene94488.t1 AT3G11030 37.500 352 178 12 30 347 106 449 3.28e-66 216
MS.gene94488.t1 AT3G06080 36.648 352 183 12 30 348 103 447 1.34e-65 214
MS.gene94488.t1 AT5G01620 32.578 353 201 9 24 346 104 449 8.05e-65 212
MS.gene94488.t1 AT5G01620 32.578 353 201 9 24 346 104 449 8.05e-65 212
MS.gene94488.t1 AT2G40160 34.930 355 187 10 30 347 77 424 9.93e-65 211
MS.gene94488.t1 AT2G40160 34.857 350 184 10 30 342 77 419 1.17e-64 211
MS.gene94488.t1 AT5G01620 32.578 353 201 9 24 346 112 457 1.29e-64 212
MS.gene94488.t1 AT5G01360 31.624 351 202 9 28 346 34 378 3.03e-64 208
MS.gene94488.t1 AT5G06230 35.043 351 199 7 25 347 16 365 8.13e-64 207
MS.gene94488.t1 AT5G01360 31.534 352 201 10 28 346 90 434 1.52e-63 208
MS.gene94488.t1 AT5G06230 35.043 351 199 7 25 347 57 406 1.61e-63 207
MS.gene94488.t1 AT3G55990 33.428 353 196 10 28 347 138 484 1.87e-63 209
MS.gene94488.t1 AT5G19160 35.311 354 188 11 30 348 99 446 3.08e-63 208
MS.gene94488.t1 AT2G31110 48.558 208 95 5 149 346 9 214 6.20e-63 200
MS.gene94488.t1 AT2G40320 34.181 354 192 10 27 347 78 423 2.72e-62 205
MS.gene94488.t1 AT1G73140 33.333 354 194 11 26 347 63 406 1.76e-61 202
MS.gene94488.t1 AT3G11570 36.250 320 177 9 55 347 101 420 1.25e-60 201
MS.gene94488.t1 AT5G58600 44.635 233 117 7 30 259 66 289 2.18e-59 193
MS.gene94488.t1 AT2G40150 32.773 357 195 10 30 347 71 421 4.27e-59 196
MS.gene94488.t1 AT3G62390 37.349 249 140 5 28 266 136 378 6.02e-57 191
MS.gene94488.t1 AT2G38320 29.577 355 211 9 28 348 57 406 3.03e-55 186
MS.gene94488.t1 AT5G20590 35.270 241 140 6 30 259 141 376 5.56e-50 171
MS.gene94488.t1 AT2G40160 38.174 241 133 6 30 260 77 311 8.92e-49 166
MS.gene94488.t1 AT2G38320 29.338 317 190 7 66 348 7 323 2.71e-48 166
MS.gene94488.t1 AT3G06080 38.272 243 130 7 30 259 103 338 2.28e-47 164
MS.gene94488.t1 AT5G19160 35.537 242 138 5 30 259 99 334 7.88e-45 157
MS.gene94488.t1 AT5G06230 33.871 248 150 4 25 259 57 303 1.53e-42 150
MS.gene94488.t1 AT5G64470 28.612 353 211 12 29 349 54 397 1.32e-38 142
MS.gene94488.t1 AT5G64470 28.134 359 211 13 29 349 54 403 1.66e-38 142
MS.gene94488.t1 AT1G70230 29.714 350 196 14 30 346 79 411 1.88e-37 139
MS.gene94488.t1 AT5G51640 28.261 368 210 12 26 349 134 491 9.88e-32 125
MS.gene94488.t1 AT4G25360 27.520 367 217 10 24 349 169 527 4.07e-30 120
MS.gene94488.t1 AT4G25360 27.520 367 217 10 24 349 169 527 4.07e-30 120
MS.gene94488.t1 AT3G28150 26.420 352 217 12 30 348 70 412 1.12e-29 118
MS.gene94488.t1 AT2G14530 26.902 368 207 13 24 346 56 406 2.20e-29 117
MS.gene94488.t1 AT5G64020 27.500 360 210 13 30 346 57 408 2.35e-28 114
MS.gene94488.t1 AT1G01430 27.224 371 216 13 20 347 89 448 6.15e-28 114
MS.gene94488.t1 AT5G20680 26.816 358 209 15 27 346 115 457 1.01e-27 113
MS.gene94488.t1 AT5G20680 26.816 358 209 15 27 346 191 533 1.35e-27 113
MS.gene94488.t1 AT5G20680 26.816 358 209 15 27 346 209 551 1.41e-27 113
MS.gene94488.t1 AT5G20680 26.816 358 209 15 27 346 209 551 1.41e-27 113
MS.gene94488.t1 AT5G20680 26.816 358 209 15 27 346 209 551 1.41e-27 113
MS.gene94488.t1 AT5G20680 26.816 358 209 15 27 346 209 551 1.41e-27 113
MS.gene94488.t1 AT2G37720 26.000 350 216 12 30 346 143 482 9.50e-26 107
MS.gene94488.t1 AT5G15900 28.415 366 190 13 30 346 67 409 8.75e-25 104
MS.gene94488.t1 AT5G15900 28.415 366 190 13 30 346 86 428 1.07e-24 104
MS.gene94488.t1 AT4G01080 26.124 356 212 11 30 348 91 432 1.44e-24 104
MS.gene94488.t1 AT5G15890 26.027 365 205 11 27 349 184 525 9.33e-24 102
MS.gene94488.t1 AT3G02440 28.249 354 205 14 30 346 127 468 1.36e-23 101
MS.gene94488.t1 AT3G02440 28.249 354 205 14 30 346 135 476 1.64e-23 101
MS.gene94488.t1 AT5G64470 27.888 251 155 9 29 262 54 295 2.19e-23 99.0
MS.gene94488.t1 AT5G64470 27.888 251 155 9 29 262 54 295 2.88e-23 99.0
MS.gene94488.t1 AT4G23790 25.946 370 221 15 21 347 69 428 1.65e-20 92.4
MS.gene94488.t1 AT4G11090 24.523 367 226 16 22 347 70 426 1.74e-17 83.6

Find 72 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAAGGTATAAGTTGAATT+TGG 0.153086 2.1:-25057616 None:intergenic
TTGCCACATGTTATTGCTTA+TGG 0.235427 2.1:-25057563 None:intergenic
AGTTGGGAATGAATTAATTA+AGG 0.268562 2.1:+25058069 MS.gene94488:CDS
AAATGGGTTCTTAGTGGATT+TGG 0.268839 2.1:+25057815 MS.gene94488:CDS
AAAGACTAGAGTCTTGTTTC+AGG 0.293533 2.1:+25058168 MS.gene94488:CDS
AACATTTCATTCCAAGTATC+AGG 0.294167 2.1:-25059283 None:intergenic
TTAAGGTATAAGTTGAATTT+GGG 0.319731 2.1:-25057615 None:intergenic
TACTAGCTTCAACATTTCTA+TGG 0.319740 2.1:+25056349 MS.gene94488:CDS
TTGCTGGTGTTCCTGATACT+TGG 0.354133 2.1:+25059272 MS.gene94488:CDS
TGTAGTCACTGGTGTCTTGC+TGG 0.365563 2.1:+25059256 MS.gene94488:CDS
AATTAAGGAGATGGATCATT+TGG 0.368405 2.1:+25058084 MS.gene94488:CDS
TCAAAGGACTGTCCTTTCAT+TGG 0.370728 2.1:+25056474 MS.gene94488:CDS
GGTGGCATTGGTCCTTGATC+TGG 0.377983 2.1:-25059091 None:intergenic
TATCATTGGTGGACTCATAC+TGG 0.389613 2.1:+25057930 MS.gene94488:CDS
CATGAAATTTCTAGTGGAAA+AGG 0.395525 2.1:+25059049 MS.gene94488:intron
GAGATTATATCAAGTATAGA+TGG 0.398174 2.1:+25056535 MS.gene94488:CDS
TTTGGTTCATGATAAAGAAA+AGG 0.409537 2.1:+25057833 MS.gene94488:CDS
AATGTTGGAATCAACCCATT+TGG 0.413535 2.1:-25058137 None:intergenic
GATTAAGCACTTGGGCCAAA+TGG 0.421751 2.1:+25058122 MS.gene94488:CDS
TTGGTTCATGATAAAGAAAA+GGG 0.424325 2.1:+25057834 MS.gene94488:CDS
AAACAAGACTCTAGTCTTTG+AGG 0.440333 2.1:-25058164 None:intergenic
ACTCACTGTGTTCTCAATTC+CGG 0.446901 2.1:+25057654 MS.gene94488:CDS
TCACTTTGCTAACACAGTTA+AGG 0.449047 2.1:+25059182 MS.gene94488:CDS
AGGAAAGAAGATAATGTTTG+TGG 0.452207 2.1:+25057534 MS.gene94488:CDS
CATGGTTGTTGTAGAGAAGT+TGG 0.454758 2.1:-25056381 None:intergenic
AACAAGACTCTAGTCTTTGA+GGG 0.459535 2.1:-25058163 None:intergenic
CAATTAGCTCAGGGAATCAA+TGG 0.459718 2.1:+25057877 MS.gene94488:CDS
AGGTTGGACACAATTAGCTC+AGG 0.471021 2.1:+25057867 MS.gene94488:CDS
ATTAAGCACTTGGGCCAAAT+GGG 0.476158 2.1:+25058123 MS.gene94488:CDS
ATGAAATTTCTAGTGGAAAA+GGG 0.480566 2.1:+25059050 MS.gene94488:intron
GTGACTACTCTCAAGGCAAC+TGG 0.482867 2.1:+25056403 MS.gene94488:CDS
GACTGTCCTTTCATTGGTCA+AGG 0.484445 2.1:+25056480 MS.gene94488:CDS
AAGACTAGAGTCTTGTTTCA+GGG 0.485326 2.1:+25058169 MS.gene94488:CDS
GACATAACTTTCGCCGCGGC+CGG 0.486529 2.1:-25059230 None:intergenic
CCGTCTATCTACACCGGCCG+CGG 0.493114 2.1:+25059217 MS.gene94488:CDS
CTAGTAGCAGAGAACATAGT+TGG 0.496126 2.1:-25056332 None:intergenic
ACAGTGTCTACACCAGGATA+AGG 0.496808 2.1:-25059112 None:intergenic
TTAAGATTGGATTAAGCACT+TGG 0.499090 2.1:+25058113 MS.gene94488:CDS
TTGTTAACAGGTTTGATGGT+GGG 0.501332 2.1:+25057491 MS.gene94488:intron
AGTCTTTGAGGGTTCAATGT+TGG 0.510727 2.1:-25058152 None:intergenic
GAATGAATTAATTAAGGAGA+TGG 0.511939 2.1:+25058075 MS.gene94488:CDS
GGAAAGAAGATAATGTTTGT+GGG 0.515523 2.1:+25057535 MS.gene94488:CDS
GGACCAATGCCACCTTATCC+TGG 0.516333 2.1:+25059100 MS.gene94488:CDS
CCCTCTCTATGATGCTTCAA+AGG 0.517294 2.1:+25056458 MS.gene94488:CDS
CCTTGAGTTGTAATCTTCCA+AGG 0.539053 2.1:+25056562 MS.gene94488:CDS
ACACCAGGATAAGGTGGCAT+TGG 0.545022 2.1:-25059103 None:intergenic
AGACAAACTAAACCAGATCA+AGG 0.552461 2.1:+25059079 MS.gene94488:CDS
CCTTGGAAGATTACAACTCA+AGG 0.555502 2.1:-25056562 None:intergenic
TTTGTTAACAGGTTTGATGG+TGG 0.560255 2.1:+25057490 MS.gene94488:intron
CTTTGCTAACACAGTTAAGG+AGG 0.560315 2.1:+25059185 MS.gene94488:CDS
GTGATGTCAAGCAATTGTAC+TGG 0.560712 2.1:-25059163 None:intergenic
ATCATAGAGAGGGTAGGAGA+AGG 0.576762 2.1:-25056448 None:intergenic
CCGCGGCCGGTGTAGATAGA+CGG 0.578901 2.1:-25059217 None:intergenic
AGCTCAGGGAATCAATGGAA+AGG 0.586442 2.1:+25057882 MS.gene94488:CDS
GTTATGTCGACTGTAGTCAC+TGG 0.597307 2.1:+25059245 MS.gene94488:CDS
CTTGGAAGATTACAACTCAA+GGG 0.597399 2.1:-25056561 None:intergenic
GGTTGGACACAATTAGCTCA+GGG 0.597836 2.1:+25057868 MS.gene94488:CDS
GTAGCAGAGAACATAGTTGG+TGG 0.598237 2.1:-25056329 None:intergenic
CTAACACAGTTAAGGAGGGA+TGG 0.604483 2.1:+25059190 MS.gene94488:CDS
TCAAAACCTTGACCAATGAA+AGG 0.604578 2.1:-25056486 None:intergenic
TCCTTTGAAGCATCATAGAG+AGG 0.605006 2.1:-25056459 None:intergenic
TAAGATTGGATTAAGCACTT+GGG 0.608859 2.1:+25058114 MS.gene94488:CDS
CCTTTGAAGCATCATAGAGA+GGG 0.612469 2.1:-25056458 None:intergenic
TGAAGCATCATAGAGAGGGT+AGG 0.612829 2.1:-25056454 None:intergenic
CAACCATGTGACTACTCTCA+AGG 0.614567 2.1:+25056396 MS.gene94488:CDS
AATATGAAGCTTCAATCATG+TGG 0.632309 2.1:+25057787 MS.gene94488:CDS
TTTGCTAACACAGTTAAGGA+GGG 0.649121 2.1:+25059186 MS.gene94488:CDS
ATTCCATAAGCAATAACATG+TGG 0.651581 2.1:+25057560 MS.gene94488:CDS
TTGCCTTGAGAGTAGTCACA+TGG 0.654201 2.1:-25056399 None:intergenic
GTGTCTACACCAGGATAAGG+TGG 0.672854 2.1:-25059109 None:intergenic
GGTCATCCGTCTATCTACAC+CGG 0.701961 2.1:+25059211 MS.gene94488:CDS
AGTCGACATAACTTTCGCCG+CGG 0.751099 2.1:-25059234 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTTAACTCAAATTT+TGG + chr2.1:25057971-25057990 MS.gene94488:intron 10.0%
!!! ATAATCAAGATTTTAACAAA+AGG - chr2.1:25058895-25058914 None:intergenic 15.0%
!!! TGAATTTTATTATGATGAAA+TGG + chr2.1:25058958-25058977 MS.gene94488:intron 15.0%
!!! TTTGTCTAATTTTGTTTTTT+GGG + chr2.1:25057751-25057770 MS.gene94488:intron 15.0%
!!! TTTTGTCTAATTTTGTTTTT+TGG + chr2.1:25057750-25057769 MS.gene94488:intron 15.0%
!! AAAAAAAAAAAAGGACAATC+CGG - chr2.1:25058411-25058430 None:intergenic 20.0%
!! AAAAACCATTCTAAGTAAAA+TGG + chr2.1:25059135-25059154 MS.gene94488:CDS 20.0%
!! ATTCAATGTTTATGTTTGAT+CGG + chr2.1:25057022-25057041 MS.gene94488:intron 20.0%
!! GAAAATAAAAACAAGAACTT+TGG - chr2.1:25056645-25056664 None:intergenic 20.0%
!! TATAATTTAATGAGTAGTAC+CGG - chr2.1:25057676-25057695 None:intergenic 20.0%
!! TTTGCATTACAAGAAAATAA+TGG + chr2.1:25057696-25057715 MS.gene94488:intron 20.0%
!!! AATTTTGGAATTTTTATGTG+AGG + chr2.1:25057986-25058005 MS.gene94488:intron 20.0%
!!! ACTGATAATTGTTTGAAATT+TGG + chr2.1:25058699-25058718 MS.gene94488:intron 20.0%
!!! TGAGTTAAAGTTTTGTTTTT+TGG + chr2.1:25056595-25056614 MS.gene94488:intron 20.0%
!!! TGATTTTCAATACATATCAT+TGG + chr2.1:25057916-25057935 MS.gene94488:CDS 20.0%
!!! TGATTTTTGTTTGAAACTAT+AGG + chr2.1:25056698-25056717 MS.gene94488:intron 20.0%
!!! TTAAGGTATAAGTTGAATTT+GGG - chr2.1:25057618-25057637 None:intergenic 20.0%
!!! TTATTTTTTAGCTTCTCTAA+TGG + chr2.1:25057465-25057484 MS.gene94488:intron 20.0%
!!! TTCTTTTGTTTGTTTTGTTA+AGG - chr2.1:25057635-25057654 None:intergenic 20.0%
!!! TTGTCTAATTTTGTTTTTTG+GGG + chr2.1:25057752-25057771 MS.gene94488:intron 20.0%
! AAATCCAAACATTCATTAAG+TGG - chr2.1:25058235-25058254 None:intergenic 25.0%
! AGTTGGGAATGAATTAATTA+AGG + chr2.1:25058069-25058088 MS.gene94488:CDS 25.0%
! CAATCATGTGGTTAAAAAAT+GGG + chr2.1:25057799-25057818 MS.gene94488:CDS 25.0%
! CATTCAAGAAAAGAAGAAAA+GGG - chr2.1:25059028-25059047 None:intergenic 25.0%
! CTCAAAAACATAGTTGAAAA+TGG - chr2.1:25056951-25056970 None:intergenic 25.0%
! GAATGAATTAATTAAGGAGA+TGG + chr2.1:25058075-25058094 MS.gene94488:CDS 25.0%
! GAGATTATATCAAGTATAGA+TGG + chr2.1:25056535-25056554 MS.gene94488:CDS 25.0%
! TAACTCAAATATAACTACCT+TGG - chr2.1:25056582-25056601 None:intergenic 25.0%
! TAGTAATTCAATGCTTATGT+TGG + chr2.1:25057157-25057176 MS.gene94488:intron 25.0%
! TATAATACACCAAAAATGGT+GGG + chr2.1:25058345-25058364 MS.gene94488:intron 25.0%
! TCAATCATGTGGTTAAAAAA+TGG + chr2.1:25057798-25057817 MS.gene94488:CDS 25.0%
! TCATTCAAGAAAAGAAGAAA+AGG - chr2.1:25059029-25059048 None:intergenic 25.0%
! TTAATCTTAGTTGTGCATAA+TGG + chr2.1:25058431-25058450 MS.gene94488:intron 25.0%
! TTGGTTCATGATAAAGAAAA+GGG + chr2.1:25057834-25057853 MS.gene94488:CDS 25.0%
!! AACCTCTTTTGTAAAAAACA+GGG + chr2.1:25058312-25058331 MS.gene94488:intron 25.0%
!! ATTTCAATGTTTTCACAGAT+GGG + chr2.1:25058036-25058055 MS.gene94488:intron 25.0%
!! CTTAAAAGCATGCATAAAAT+GGG + chr2.1:25058460-25058479 MS.gene94488:intron 25.0%
!! CTTAATGAATGTTTGGATTT+TGG + chr2.1:25058235-25058254 MS.gene94488:intron 25.0%
!! GTTAAGGTATAAGTTGAATT+TGG - chr2.1:25057619-25057638 None:intergenic 25.0%
!! TATTTCAATGTTTTCACAGA+TGG + chr2.1:25058035-25058054 MS.gene94488:intron 25.0%
!! TTTGGAATGTATCTTCAAAT+GGG + chr2.1:25056613-25056632 MS.gene94488:intron 25.0%
!! TTTGGTTCATGATAAAGAAA+AGG + chr2.1:25057833-25057852 MS.gene94488:CDS 25.0%
!! TTTTCAATACATATCATTGG+TGG + chr2.1:25057919-25057938 MS.gene94488:CDS 25.0%
!!! AAGTTTTTGGAAAGATACAA+AGG + chr2.1:25057514-25057533 MS.gene94488:CDS 25.0%
!!! ACATAGTTGACTATTTTTAG+TGG + chr2.1:25056920-25056939 MS.gene94488:intron 25.0%
!!! ATGAAATTTCTAGTGGAAAA+GGG + chr2.1:25059050-25059069 MS.gene94488:intron 25.0%
!!! CATAGTTGACTATTTTTAGT+GGG + chr2.1:25056921-25056940 MS.gene94488:intron 25.0%
!!! TGGATTTTGGTTATTAAAGT+TGG + chr2.1:25058248-25058267 MS.gene94488:intron 25.0%
!!! TTTGTTGTTGTTATTGTTGT+TGG + chr2.1:25056837-25056856 MS.gene94488:intron 25.0%
!!! TTTTGGAATGTATCTTCAAA+TGG + chr2.1:25056612-25056631 MS.gene94488:intron 25.0%
AACATTTCATTCCAAGTATC+AGG - chr2.1:25059286-25059305 None:intergenic 30.0%
AATATGAAGCTTCAATCATG+TGG + chr2.1:25057787-25057806 MS.gene94488:CDS 30.0%
AATTAAGGAGATGGATCATT+TGG + chr2.1:25058084-25058103 MS.gene94488:CDS 30.0%
AGACTTAGATGAATGTAATG+TGG + chr2.1:25057404-25057423 MS.gene94488:intron 30.0%
AGGAAAGAAGATAATGTTTG+TGG + chr2.1:25057534-25057553 MS.gene94488:CDS 30.0%
ATAGTCAATCATCCACAAAT+CGG - chr2.1:25056889-25056908 None:intergenic 30.0%
ATTCCATAAGCAATAACATG+TGG + chr2.1:25057560-25057579 MS.gene94488:CDS 30.0%
ATTCTCCTTAGCATCAATTT+CGG + chr2.1:25057228-25057247 MS.gene94488:intron 30.0%
GGAAAGAAGATAATGTTTGT+GGG + chr2.1:25057535-25057554 MS.gene94488:CDS 30.0%
GTATAATACACCAAAAATGG+TGG + chr2.1:25058344-25058363 MS.gene94488:intron 30.0%
TAAGATTGGATTAAGCACTT+GGG + chr2.1:25058114-25058133 MS.gene94488:CDS 30.0%
TATATGTGATAGTTTCGTGT+CGG + chr2.1:25058745-25058764 MS.gene94488:intron 30.0%
TCTTCCACTTAATGAATGTT+TGG + chr2.1:25058228-25058247 MS.gene94488:intron 30.0%
TGCGTATAATACACCAAAAA+TGG + chr2.1:25058341-25058360 MS.gene94488:intron 30.0%
TTAAGATTGGATTAAGCACT+TGG + chr2.1:25058113-25058132 MS.gene94488:CDS 30.0%
TTCTGCATGAATTATAAGCA+AGG + chr2.1:25058542-25058561 MS.gene94488:intron 30.0%
TTTAAACTCGACACATTGTA+AGG + chr2.1:25057099-25057118 MS.gene94488:intron 30.0%
! AAGAAAAGGGAAGAATTTTG+AGG + chr2.1:25057847-25057866 MS.gene94488:CDS 30.0%
! CAACCTCTTTTGTAAAAAAC+AGG + chr2.1:25058311-25058330 MS.gene94488:intron 30.0%
! CTCTAATGGTTTTTGTTAAC+AGG + chr2.1:25057479-25057498 MS.gene94488:intron 30.0%
! CTTCTCCATTTTACTTAGAA+TGG - chr2.1:25059143-25059162 None:intergenic 30.0%
! CTTTTGTAAAAAACAGGGTA+AGG + chr2.1:25058317-25058336 MS.gene94488:intron 30.0%
! GAATGACATGAAATTTCTAG+TGG + chr2.1:25059043-25059062 MS.gene94488:intron 30.0%
! GCTTAAAAGCATGCATAAAA+TGG + chr2.1:25058459-25058478 MS.gene94488:intron 30.0%
! GTTAAAAAATGGGTTCTTAG+TGG + chr2.1:25057809-25057828 MS.gene94488:CDS 30.0%
! GTTTTTGTTAACAGGTTTGA+TGG + chr2.1:25057487-25057506 MS.gene94488:intron 30.0%
! TACTAGCTTCAACATTTCTA+TGG + chr2.1:25056349-25056368 MS.gene94488:CDS 30.0%
! TTGTTGTTGGTGTATGATAT+TGG + chr2.1:25056850-25056869 MS.gene94488:intron 30.0%
!! AAGTTTTGTCTCACTAAATG+AGG - chr2.1:25056819-25056838 None:intergenic 30.0%
!! CATGAAATTTCTAGTGGAAA+AGG + chr2.1:25059049-25059068 MS.gene94488:intron 30.0%
!!! AGATGGGATTATTTTCAAGT+TGG + chr2.1:25058052-25058071 MS.gene94488:intron 30.0%
!!! CATTTGGAAGCTTTTAAGAT+TGG + chr2.1:25058100-25058119 MS.gene94488:CDS 30.0%
!!! GATGGGATTATTTTCAAGTT+GGG + chr2.1:25058053-25058072 MS.gene94488:intron 30.0%
!!! GTTTTTTGGGGTGTTTTTAT+AGG + chr2.1:25057764-25057783 MS.gene94488:intron 30.0%
!!! TACCCTGTTTTTTACAAAAG+AGG - chr2.1:25058317-25058336 None:intergenic 30.0%
!!! TTCAACTATGTTTTTGAGAC+CGG + chr2.1:25056953-25056972 MS.gene94488:intron 30.0%
AATGTTGGAATCAACCCATT+TGG - chr2.1:25058140-25058159 None:intergenic 35.0%
ACATTAAACCCTCAATTCCA+CGG + chr2.1:25058851-25058870 MS.gene94488:intron 35.0%
ACCACAATTGAGACTTCATT+TGG - chr2.1:25058626-25058645 None:intergenic 35.0%
AGACAAACTAAACCAGATCA+AGG + chr2.1:25059079-25059098 MS.gene94488:CDS 35.0%
ATCTTAGTTGTGCATAATGG+TGG + chr2.1:25058434-25058453 MS.gene94488:intron 35.0%
CTTCAAATTAAAGTCGTGTC+CGG - chr2.1:25058789-25058808 None:intergenic 35.0%
CTTGGAAGATTACAACTCAA+GGG - chr2.1:25056564-25056583 None:intergenic 35.0%
TCAAAACCTTGACCAATGAA+AGG - chr2.1:25056489-25056508 None:intergenic 35.0%
TCACTTTGCTAACACAGTTA+AGG + chr2.1:25059182-25059201 MS.gene94488:CDS 35.0%
TTGCCACATGTTATTGCTTA+TGG - chr2.1:25057566-25057585 None:intergenic 35.0%
TTTGCTAACACAGTTAAGGA+GGG + chr2.1:25059186-25059205 MS.gene94488:CDS 35.0%
TTTGTTAACAGGTTTGATGG+TGG + chr2.1:25057490-25057509 MS.gene94488:intron 35.0%
! AAACAAGACTCTAGTCTTTG+AGG - chr2.1:25058167-25058186 None:intergenic 35.0%
! AACAAGACTCTAGTCTTTGA+GGG - chr2.1:25058166-25058185 None:intergenic 35.0%
! CCTTGTGATGGAAAAAAATG+TGG - chr2.1:25057061-25057080 None:intergenic 35.0%
! TTGTTAACAGGTTTGATGGT+GGG + chr2.1:25057491-25057510 MS.gene94488:intron 35.0%
!! AAAGACTAGAGTCTTGTTTC+AGG + chr2.1:25058168-25058187 MS.gene94488:CDS 35.0%
!! AAATGGGTTCTTAGTGGATT+TGG + chr2.1:25057815-25057834 MS.gene94488:CDS 35.0%
!! AAGACTAGAGTCTTGTTTCA+GGG + chr2.1:25058169-25058188 MS.gene94488:CDS 35.0%
!! ATGAAGTTTCAACCGATTTG+TGG + chr2.1:25056874-25056893 MS.gene94488:intron 35.0%
!!! AAAGGGAAGAATTTTGAGGT+TGG + chr2.1:25057851-25057870 MS.gene94488:CDS 35.0%
!!! CCACATTTTTTTCCATCACA+AGG + chr2.1:25057058-25057077 MS.gene94488:intron 35.0%
!!! GGTTTTGATTGTCTTGGAAA+TGG + chr2.1:25056501-25056520 MS.gene94488:CDS 35.0%
!!! TCAACTATGTTTTTGAGACC+GGG + chr2.1:25056954-25056973 MS.gene94488:intron 35.0%
ACTCACTGTGTTCTCAATTC+CGG + chr2.1:25057654-25057673 MS.gene94488:CDS 40.0%
ATGTGAGGAATTTGAGCAAG+TGG + chr2.1:25058001-25058020 MS.gene94488:intron 40.0%
ATTAAGCACTTGGGCCAAAT+GGG + chr2.1:25058123-25058142 MS.gene94488:CDS 40.0%
CAATTAGCTCAGGGAATCAA+TGG + chr2.1:25057877-25057896 MS.gene94488:CDS 40.0%
CATGGTTGTTGTAGAGAAGT+TGG - chr2.1:25056384-25056403 None:intergenic 40.0%
CCTTGGAAGATTACAACTCA+AGG - chr2.1:25056565-25056584 None:intergenic 40.0%
CTAGTAGCAGAGAACATAGT+TGG - chr2.1:25056335-25056354 None:intergenic 40.0%
CTCGAGTTTGATTCTTGTGA+TGG - chr2.1:25057356-25057375 None:intergenic 40.0%
CTTTGCTAACACAGTTAAGG+AGG + chr2.1:25059185-25059204 MS.gene94488:CDS 40.0%
GAATTTGAGCAAGTGGCTAA+TGG + chr2.1:25058008-25058027 MS.gene94488:intron 40.0%
GATCTGAGTCTGATTACACT+TGG + chr2.1:25056976-25056995 MS.gene94488:intron 40.0%
GCCAAATGAAGTCTCAATTG+TGG + chr2.1:25058622-25058641 MS.gene94488:intron 40.0%
GTGATGTCAAGCAATTGTAC+TGG - chr2.1:25059166-25059185 None:intergenic 40.0%
TCAAAGGACTGTCCTTTCAT+TGG + chr2.1:25056474-25056493 MS.gene94488:CDS 40.0%
! AGTCTTTGAGGGTTCAATGT+TGG - chr2.1:25058155-25058174 None:intergenic 40.0%
! CCTTGAGTTGTAATCTTCCA+AGG + chr2.1:25056562-25056581 MS.gene94488:CDS 40.0%
! GTTTTATGCAGTCATGGATG+TGG + chr2.1:25058564-25058583 MS.gene94488:intron 40.0%
! GTTTTTACAGTGTCTACACC+AGG - chr2.1:25059121-25059140 None:intergenic 40.0%
! TTCTTGTGATGGAAACAACG+TGG - chr2.1:25057345-25057364 None:intergenic 40.0%
!! AGCAAGGTTTTATGCAGTCA+TGG + chr2.1:25058558-25058577 MS.gene94488:intron 40.0%
!! CCTTTGAAGCATCATAGAGA+GGG - chr2.1:25056461-25056480 None:intergenic 40.0%
!! TATCATTGGTGGACTCATAC+TGG + chr2.1:25057930-25057949 MS.gene94488:CDS 40.0%
!! TCCTTTGAAGCATCATAGAG+AGG - chr2.1:25056462-25056481 None:intergenic 40.0%
!!! GGTCAAGGTTTTGATTGTCT+TGG + chr2.1:25056495-25056514 MS.gene94488:CDS 40.0%
!!! GTTTGATGGTGGGAAGTTTT+TGG + chr2.1:25057501-25057520 MS.gene94488:CDS 40.0%
AATCTGAGCCGTGGAATTGA+GGG - chr2.1:25058862-25058881 None:intergenic 45.0%
ACAGTGTCTACACCAGGATA+AGG - chr2.1:25059115-25059134 None:intergenic 45.0%
AGCTCAGGGAATCAATGGAA+AGG + chr2.1:25057882-25057901 MS.gene94488:CDS 45.0%
AGGTTGGACACAATTAGCTC+AGG + chr2.1:25057867-25057886 MS.gene94488:CDS 45.0%
ATCATAGAGAGGGTAGGAGA+AGG - chr2.1:25056451-25056470 None:intergenic 45.0%
CAACCATGTGACTACTCTCA+AGG + chr2.1:25056396-25056415 MS.gene94488:CDS 45.0%
CCCTCTCTATGATGCTTCAA+AGG + chr2.1:25056458-25056477 MS.gene94488:CDS 45.0%
CTAACACAGTTAAGGAGGGA+TGG + chr2.1:25059190-25059209 MS.gene94488:CDS 45.0%
CTCCTTAGCATCAATTTCGG+CGG + chr2.1:25057231-25057250 MS.gene94488:intron 45.0%
GACTGTCCTTTCATTGGTCA+AGG + chr2.1:25056480-25056499 MS.gene94488:CDS 45.0%
GATTAAGCACTTGGGCCAAA+TGG + chr2.1:25058122-25058141 MS.gene94488:CDS 45.0%
GCTGCAATCTATGTAGCACT+GGG - chr2.1:25058813-25058832 None:intergenic 45.0%
GGTTGGACACAATTAGCTCA+GGG + chr2.1:25057868-25057887 MS.gene94488:CDS 45.0%
GTAGCAGAGAACATAGTTGG+TGG - chr2.1:25056332-25056351 None:intergenic 45.0%
GTCATGTGACTGAAAGGTCA+CGG + chr2.1:25058272-25058291 MS.gene94488:intron 45.0%
GTGTAATCAGACTCAGATCC+CGG - chr2.1:25056975-25056994 None:intergenic 45.0%
TCATGTGACTGAAAGGTCAC+GGG + chr2.1:25058273-25058292 MS.gene94488:intron 45.0%
TCCTTAGCATCAATTTCGGC+GGG + chr2.1:25057232-25057251 MS.gene94488:intron 45.0%
TTGCCTTGAGAGTAGTCACA+TGG - chr2.1:25056402-25056421 None:intergenic 45.0%
TTGCTGGTGTTCCTGATACT+TGG + chr2.1:25059272-25059291 MS.gene94488:CDS 45.0%
! AAGGTCACGGGTTAAAGTCA+TGG + chr2.1:25058285-25058304 MS.gene94488:intron 45.0%
! GTTATGTCGACTGTAGTCAC+TGG + chr2.1:25059245-25059264 MS.gene94488:CDS 45.0%
! TGAAGCATCATAGAGAGGGT+AGG - chr2.1:25056457-25056476 None:intergenic 45.0%
! TGTGCTACTCTCAACTCTCA+AGG + chr2.1:25057195-25057214 MS.gene94488:intron 45.0%
!! GTTGGTGTCATGTGACTGAA+AGG + chr2.1:25058266-25058285 MS.gene94488:intron 45.0%
!! TAAGATTAAAAAAAAAAAAA+AGG - chr2.1:25058420-25058439 None:intergenic 5.0%
ACACCAGGATAAGGTGGCAT+TGG - chr2.1:25059106-25059125 None:intergenic 50.0%
ACTAAAGCTCGCGCATATGC+AGG - chr2.1:25058385-25058404 None:intergenic 50.0%
AGTCGACATAACTTTCGCCG+CGG - chr2.1:25059237-25059256 None:intergenic 50.0%
ATGTAGCCACGATTCGCAAC+AGG - chr2.1:25058835-25058854 None:intergenic 50.0%
CACAACTAGAATCTGAGCCG+TGG - chr2.1:25058871-25058890 None:intergenic 50.0%
CTAAAGCTCGCGCATATGCA+GGG - chr2.1:25058384-25058403 None:intergenic 50.0%
GAATCTGAGCCGTGGAATTG+AGG - chr2.1:25058863-25058882 None:intergenic 50.0%
GAGAGTTGAGAGTAGCACAC+TGG - chr2.1:25057194-25057213 None:intergenic 50.0%
GGCTGCAATCTATGTAGCAC+TGG - chr2.1:25058814-25058833 None:intergenic 50.0%
GGTCATCCGTCTATCTACAC+CGG + chr2.1:25059211-25059230 MS.gene94488:CDS 50.0%
GTGACTACTCTCAAGGCAAC+TGG + chr2.1:25056403-25056422 MS.gene94488:CDS 50.0%
GTGTCTACACCAGGATAAGG+TGG - chr2.1:25059112-25059131 None:intergenic 50.0%
GTTGAGAGTAGCACACTGGA+AGG - chr2.1:25057190-25057209 None:intergenic 50.0%
TGTAGTCACTGGTGTCTTGC+TGG + chr2.1:25059256-25059275 MS.gene94488:CDS 50.0%
! ATGCGCGAGCTTTAGTACAC+CGG + chr2.1:25058389-25058408 MS.gene94488:intron 50.0%
! CTCGAGTTTGAGCCTTGTGA+TGG - chr2.1:25057073-25057092 None:intergenic 50.0%
GCCCGCCGAAATTGATGCTA+AGG - chr2.1:25057236-25057255 None:intergenic 55.0%
GGACCAATGCCACCTTATCC+TGG + chr2.1:25059100-25059119 MS.gene94488:CDS 55.0%
GTGTCCAACTCTCTCTGGAC+CGG + chr2.1:25058767-25058786 MS.gene94488:intron 55.0%
TTGCAGCCTGTTGCGAATCG+TGG + chr2.1:25058826-25058845 MS.gene94488:intron 55.0%
! AAAATGGTGGGACCCCTTCC+CGG + chr2.1:25058357-25058376 MS.gene94488:intron 55.0%
! GGAAGGGGTCCCACCATTTT+TGG - chr2.1:25058357-25058376 None:intergenic 55.0%
! TGTCGGTGTCCAACTCTCTC+TGG + chr2.1:25058762-25058781 MS.gene94488:intron 55.0%
!! GGTGGCATTGGTCCTTGATC+TGG - chr2.1:25059094-25059113 None:intergenic 55.0%
CATATGCAGGGTCCGGGAAG+GGG - chr2.1:25058372-25058391 None:intergenic 60.0%
GACATAACTTTCGCCGCGGC+CGG - chr2.1:25059233-25059252 None:intergenic 60.0%
GCATATGCAGGGTCCGGGAA+GGG - chr2.1:25058373-25058392 None:intergenic 60.0%
GTGTCCGGTCCAGAGAGAGT+TGG - chr2.1:25058774-25058793 None:intergenic 60.0%
CCGCGGCCGGTGTAGATAGA+CGG - chr2.1:25059220-25059239 None:intergenic 65.0%
CCGTCTATCTACACCGGCCG+CGG + chr2.1:25059217-25059236 MS.gene94488:CDS 65.0%
CGCATATGCAGGGTCCGGGA+AGG - chr2.1:25058374-25058393 None:intergenic 65.0%
CTCGCGCATATGCAGGGTCC+GGG - chr2.1:25058378-25058397 None:intergenic 65.0%
GCTCGCGCATATGCAGGGTC+CGG - chr2.1:25058379-25058398 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 25056315 25059330 25056315 ID=MS.gene94488
chr2.1 mRNA 25056315 25059330 25056315 ID=MS.gene94488.t1;Parent=MS.gene94488
chr2.1 exon 25056315 25056583 25056315 ID=MS.gene94488.t1.exon1;Parent=MS.gene94488.t1
chr2.1 CDS 25056315 25056583 25056315 ID=cds.MS.gene94488.t1;Parent=MS.gene94488.t1
chr2.1 exon 25057501 25057675 25057501 ID=MS.gene94488.t1.exon2;Parent=MS.gene94488.t1
chr2.1 CDS 25057501 25057675 25057501 ID=cds.MS.gene94488.t1;Parent=MS.gene94488.t1
chr2.1 exon 25057786 25057964 25057786 ID=MS.gene94488.t1.exon3;Parent=MS.gene94488.t1
chr2.1 CDS 25057786 25057964 25057786 ID=cds.MS.gene94488.t1;Parent=MS.gene94488.t1
chr2.1 exon 25058054 25058212 25058054 ID=MS.gene94488.t1.exon4;Parent=MS.gene94488.t1
chr2.1 CDS 25058054 25058212 25058054 ID=cds.MS.gene94488.t1;Parent=MS.gene94488.t1
chr2.1 exon 25059063 25059330 25059063 ID=MS.gene94488.t1.exon5;Parent=MS.gene94488.t1
chr2.1 CDS 25059063 25059330 25059063 ID=cds.MS.gene94488.t1;Parent=MS.gene94488.t1
Gene Sequence

>MS.gene94488

ATGTCACTCACATTCCACCAACTATGTTCTCTGCTACTAGCTTCAACATTTCTATGGACACTACTACCAACTTCTCTACAACAACCATGTGACTACTCTCAAGGCAACTGGCTCATTGATGATGATTTCACTACCTTCTCCTACCCTCTCTATGATGCTTCAAAGGACTGTCCTTTCATTGGTCAAGGTTTTGATTGTCTTGGAAATGGTAGAACTGATAGAGATTATATCAAGTATAGATGGAAGCCCTTGAGTTGTAATCTTCCAAGGTAGTTATATTTGAGTTAAAGTTTTGTTTTTTGGAATGTATCTTCAAATGGGTAGTGACCAAAGTTCTTGTTTTTATTTTCAGTTTTTATGTTTTTTTGTTGTTGTTGTTAGTATGATTTTTGTTTGAAACTATAGGAGAAAGTGATAAGAAAAATATAAAAAAATTAATGAGTTTGATCGACATGTGATATATGATTGAACATTATGACTTTGTTTGATCAATGTTGTCGACCTCATTTAGTGAGACAAAACTTTGTTGTTGTTATTGTTGTTGGTGTATGATATTGGTATGAAGTTTCAACCGATTTGTGGATGATTGACTATGTTTTTCGAGAACATAGTTGACTATTTTTAGTGGGATCTCCATTTTCAACTATGTTTTTGAGACCGGGATCTGAGTCTGATTACACTTGGACTAATGTAATGTAATGTTGTTAATTCAATGTTTATGTTTGATCGGCAACAAAAGTTAGCCACATTTTTTTCCATCACAAGGCTCAAACTCGAGACATTGTTTAAACTCGACACATTGTAAGGATCTGAGTCTAGTTAGACTCAGATGAATGTAATGTTAGTAATTCAATGCTTATGTTGGCTAGAGACCTTCCAGTGTGCTACTCTCAACTCTCAAGGTCTCAATTCAATTCTCCTTAGCATCAATTTCGGCGGGCTAGTTTAGCTTCTTCAAAAAAAAAATGTTTCTGTTTACTTAGCAAAAAAGTAAACATTCTTTTGCTTACATAATGACTTTTGTTAACCACGTTGTTTCCATCACAAGAATCAAACTCGAGACTTTGTTTAAGAAATCTGAGTCTAGTTAGACTTAGATGAATGTAATGTGGATAATACAATTTTCAAGTTTGATTAATAAAATTAGAACTTATTTTTTAGCTTCTCTAATGGTTTTTGTTAACAGGTTTGATGGTGGGAAGTTTTTGGAAAGATACAAAGGAAAGAAGATAATGTTTGTGGGAGATTCCATAAGCAATAACATGTGGCAATCACTCACTTGTTTGCTTCACATTGCAATCCCAAATTCAACTTATACCTTAACAAAACAAACAAAAGAACTCACTGTGTTCTCAATTCCGGTACTACTCATTAAATTATATTTGCATTACAAGAAAATAATGGATTTTATCTAATTGTTTGATTAGTTTCATAATTTTGTCTAATTTTGTTTTTTGGGGTGTTTTTATAGGAATATGAAGCTTCAATCATGTGGTTAAAAAATGGGTTCTTAGTGGATTTGGTTCATGATAAAGAAAAGGGAAGAATTTTGAGGTTGGACACAATTAGCTCAGGGAATCAATGGAAAGGTTTTGATGTTTTGATTTTCAATACATATCATTGGTGGACTCATACTGGAAAATCTCAAACGTATTGAAATTTTTTAACTCAAATTTTGGAATTTTTATGTGAGGAATTTGAGCAAGTGGCTAATGGTTTTTATTTCAATGTTTTCACAGATGGGATTATTTTCAAGTTGGGAATGAATTAATTAAGGAGATGGATCATTTGGAAGCTTTTAAGATTGGATTAAGCACTTGGGCCAAATGGGTTGATTCCAACATTGAACCCTCAAAGACTAGAGTCTTGTTTCAGGGAATTGCTGCTTCTCATGTTGAGTAAGTTTAACTGTTTCTTCCACTTAATGAATGTTTGGATTTTGGTTATTAAAGTTGGTGTCATGTGACTGAAAGGTCACGGGTTAAAGTCATGGAAACAACCTCTTTTGTAAAAAACAGGGTAAGGTTGCGTATAATACACCAAAAATGGTGGGACCCCTTCCCGGACCCTGCATATGCGCGAGCTTTAGTACACCGGATTGTCCTTTTTTTTTTTTTTAATCTTAGTTGTGCATAATGGTGGTTGCTTAAAAGCATGCATAAAATGGGTCTAAATTTTCAAAGTATGTGCTAACTATATGATTATGTGTGCAACTTAACAAACTCAATTCTGCATGAATTATAAGCAAGGTTTTATGCAGTCATGGATGTGGTCACATTACAATAATTGATATTGCAGAAAATTTCAGCCAAATGAAGTCTCAATTGTGGTCTATGAAGCACTGTCATAGATGCATATACTAGATACGACACTGACACATCGACACTGATAATTGTTTGAAATTTGGAAGAAAAAAAAATGAATGTAAATTATATGTGATAGTTTCGTGTCGGTGTCCAACTCTCTCTGGACCGGACACGACTTTAATTTGAAGTCCCAGTGCTACATAGATTGCAGCCTGTTGCGAATCGTGGCTACATTAAACCCTCAATTCCACGGCTCAGATTCTAGTTGTGACCTTTTGTTAAAATCTTGATTATAAGTATTATGTGAAGAGAGATCATAGAAAGACATTTGAGACACTGAATTTTATTATGATGAAATGGTAATATTGATTATGAAAGTATACTCAAAAGACTCACTCTTTTTTCCCTTTTCTTCTTTTCTTGAATGACATGAAATTTCTAGTGGAAAAGGGTGCTTGAGACAAACTAAACCAGATCAAGGACCAATGCCACCTTATCCTGGTGTAGACACTGTAAAAACCATTCTAAGTAAAATGGAGAAGCCAGTACAATTGCTTGACATCACTTTGCTAACACAGTTAAGGAGGGATGGTCATCCGTCTATCTACACCGGCCGCGGCGAAAGTTATGTCGACTGTAGTCACTGGTGTCTTGCTGGTGTTCCTGATACTTGGAATGAAATGTTGTATTCTGCTCTTCTTGAAGATTAG
Protein sequence

>MS.gene94488.t1

MSLTFHQLCSLLLASTFLWTLLPTSLQQPCDYSQGNWLIDDDFTTFSYPLYDASKDCPFIGQGFDCLGNGRTDRDYIKYRWKPLSCNLPRFDGGKFLERYKGKKIMFVGDSISNNMWQSLTCLLHIAIPNSTYTLTKQTKELTVFSIPEYEASIMWLKNGFLVDLVHDKEKGRILRLDTISSGNQWKGFDVLIFNTYHWWTHTGKSQTWDYFQVGNELIKEMDHLEAFKIGLSTWAKWVDSNIEPSKTRVLFQGIAASHVDGKGCLRQTKPDQGPMPPYPGVDTVKTILSKMEKPVQLLDITLLTQLRRDGHPSIYTGRGESYVDCSHWCLAGVPDTWNEMLYSALLED