AlfalfaGEDB | MS.gene94622

M. sativa cultivar XinJiangDaYe / MS.gene94622

KEGG: K09646
GO: GO:0003674 GO:0003824 GO:0004180 GO:0004185 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0016787 GO:0016825 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070008 GO:0070011 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00450.25

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94622.t1	XP_013464874.1	93.4	456	29	1	1	456	1	455	5.20E-250	873.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94622.t1	Q67Y83	61.0	431	165	2	28	455	29	459	5.0e-153	542.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94622.t1	A0A072VA69	93.4	456	29	1	1	456	1	455	3.7e-250	873.2

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene020426	MS.gene94622	PPI
MS.gene022111	MS.gene94622	PPI
MS.gene43592	MS.gene94622	PPI
MS.gene94622	MS.gene004371	PPI
MS.gene94622	MS.gene05804	PPI
MS.gene94622	MS.gene80760	PPI
MS.gene94622	MS.gene05805	PPI
MS.gene94622	MS.gene037763	PPI
MS.gene94622	MS.gene002689	PPI
MS.gene94622	MS.gene05803	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94622.t1	MTR_2g084530	93.217	457	30	1	1	457	1	456	0.0	843
MS.gene94622.t1	MTR_4g056520	68.696	460	139	4	1	457	1	458	0.0	624
MS.gene94622.t1	MTR_1g050545	28.972	428	244	15	38	448	90	474	3.14e-34	134
MS.gene94622.t1	MTR_1g094155	25.747	435	268	13	29	448	86	480	1.24e-32	130
MS.gene94622.t1	MTR_4g057595	26.327	452	281	13	11	449	76	488	1.36e-32	129
MS.gene94622.t1	MTR_4g057585	26.281	449	275	15	16	449	80	487	2.44e-32	129
MS.gene94622.t1	MTR_2g095730	27.529	425	245	15	44	448	107	488	3.00e-32	129
MS.gene94622.t1	MTR_4g057600	25.659	417	259	12	44	448	108	485	6.85e-32	127
MS.gene94622.t1	MTR_4g057570	26.244	442	269	11	27	450	83	485	1.56e-31	126
MS.gene94622.t1	MTR_2g095750	27.685	419	249	12	44	448	113	491	5.27e-31	125

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94622.t1	AT2G27920	61.663	433	163	2	28	457	29	461	0.0	550
MS.gene94622.t1	AT2G27920	59.067	386	155	2	75	457	4	389	4.21e-160	458
MS.gene94622.t1	AT2G27920	57.349	347	145	2	114	457	48	394	5.17e-136	397
MS.gene94622.t1	AT3G45010	26.087	437	283	10	20	448	88	492	1.92e-36	140
MS.gene94622.t1	AT3G10410	27.857	420	245	13	44	448	112	488	9.00e-33	130
MS.gene94622.t1	AT5G22980	25.899	417	257	10	44	448	111	487	4.95e-32	128
MS.gene94622.t1	AT1G15000	26.279	430	250	14	36	448	38	417	3.12e-31	125
MS.gene94622.t1	AT4G15100	26.185	443	275	14	36	453	61	476	6.08e-25	107
MS.gene94622.t1	AT1G11080	26.386	451	260	16	36	450	67	481	6.19e-23	101
MS.gene94622.t1	AT1G11080	26.386	451	260	16	36	450	67	481	6.50e-23	101

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Find 91 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCAGATTTGCTATTTG+TGG	0.380443	2.4:-14899943	MS.gene94622:intron
AAACTGAACCCTGTACCAAC+TGG	0.435888	2.4:+14899646	None:intergenic
AACCAGAGAATGATTGGCCA+TGG	0.561866	2.4:+14900196	None:intergenic
AACCATGGCCAATCATTCTC+TGG	0.471827	2.4:-14900198	MS.gene94622:CDS
AATGATTGGCCATGGTTTGG+AGG	0.489481	2.4:+14900204	None:intergenic
AATTGCTGACAGATGGTATG+CGG	0.759275	2.4:-14898128	MS.gene94622:intron
ACCATGTTTAGTGCTATGCA+GGG	0.650772	2.4:+14897370	None:intergenic
ACTTGTCAAAACTGATGAGG+AGG	0.613052	2.4:-14899605	MS.gene94622:CDS
AGATTGAAGACTACACTTGG+AGG	0.634315	2.4:-14899433	MS.gene94622:intron
AGGACAATCCAGTTGGTACA+GGG	0.637361	2.4:-14899654	MS.gene94622:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACCCGATCAATTTAAAAGTT+TGG	+	chr2.4:14897883-14897902	None:intergenic	30.0%
AGAAAAGAACAATCGGTATT+TGG	+	chr2.4:14900552-14900571	None:intergenic	30.0%
ATAATATAGGACAATCCAGT+TGG	-	chr2.4:14898722-14898741	MS.gene94622:intron	30.0%
ATCTCATGTTTGTGTGTTTA+AGG	-	chr2.4:14899943-14899962	MS.gene94622:intron	30.0%
ATTACTTGTCAAAACTGATG+AGG	-	chr2.4:14898775-14898794	MS.gene94622:CDS	30.0%
ATTGCAAGAAACATGAGATT+AGG	+	chr2.4:14900238-14900257	None:intergenic	30.0%
CAAGTAACCAATAGAAGTTT+AGG	+	chr2.4:14900868-14900887	None:intergenic	30.0%
CATGATTAACACTAGAAGAT+GGG	+	chr2.4:14899678-14899697	None:intergenic	30.0%
CTTCATAAAATAGGAAACCA+AGG	+	chr2.4:14897380-14897399	None:intergenic	30.0%
CTTTGTGACACCATTTAATA+AGG	+	chr2.4:14900122-14900141	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	14897339	14901066	14897339	ID=MS.gene94622
chr2.4	mRNA	14897339	14901066	14897339	ID=MS.gene94622.t1;Parent=MS.gene94622
chr2.4	exon	14900940	14901066	14900940	ID=MS.gene94622.t1.exon1;Parent=MS.gene94622.t1
chr2.4	CDS	14900940	14901066	14900940	ID=cds.MS.gene94622.t1;Parent=MS.gene94622.t1
chr2.4	exon	14900183	14900277	14900183	ID=MS.gene94622.t1.exon2;Parent=MS.gene94622.t1
chr2.4	CDS	14900183	14900277	14900183	ID=cds.MS.gene94622.t1;Parent=MS.gene94622.t1
chr2.4	exon	14899944	14900051	14899944	ID=MS.gene94622.t1.exon3;Parent=MS.gene94622.t1
chr2.4	CDS	14899944	14900051	14899944	ID=cds.MS.gene94622.t1;Parent=MS.gene94622.t1
chr2.4	exon	14899434	14899674	14899434	ID=MS.gene94622.t1.exon4;Parent=MS.gene94622.t1
chr2.4	CDS	14899434	14899674	14899434	ID=cds.MS.gene94622.t1;Parent=MS.gene94622.t1
chr2.4	exon	14898906	14898949	14898906	ID=MS.gene94622.t1.exon5;Parent=MS.gene94622.t1
chr2.4	CDS	14898906	14898949	14898906	ID=cds.MS.gene94622.t1;Parent=MS.gene94622.t1
chr2.4	exon	14898753	14898820	14898753	ID=MS.gene94622.t1.exon6;Parent=MS.gene94622.t1
chr2.4	CDS	14898753	14898820	14898753	ID=cds.MS.gene94622.t1;Parent=MS.gene94622.t1
chr2.4	exon	14898545	14898650	14898545	ID=MS.gene94622.t1.exon7;Parent=MS.gene94622.t1
chr2.4	CDS	14898545	14898650	14898545	ID=cds.MS.gene94622.t1;Parent=MS.gene94622.t1
chr2.4	exon	14898232	14898440	14898232	ID=MS.gene94622.t1.exon8;Parent=MS.gene94622.t1
chr2.4	CDS	14898232	14898440	14898232	ID=cds.MS.gene94622.t1;Parent=MS.gene94622.t1
chr2.4	exon	14898069	14898138	14898069	ID=MS.gene94622.t1.exon9;Parent=MS.gene94622.t1
chr2.4	CDS	14898069	14898138	14898069	ID=cds.MS.gene94622.t1;Parent=MS.gene94622.t1
chr2.4	exon	14897891	14897941	14897891	ID=MS.gene94622.t1.exon10;Parent=MS.gene94622.t1
chr2.4	CDS	14897891	14897941	14897891	ID=cds.MS.gene94622.t1;Parent=MS.gene94622.t1
chr2.4	exon	14897741	14897793	14897741	ID=MS.gene94622.t1.exon11;Parent=MS.gene94622.t1
chr2.4	CDS	14897741	14897793	14897741	ID=cds.MS.gene94622.t1;Parent=MS.gene94622.t1
chr2.4	exon	14897504	14897636	14897504	ID=MS.gene94622.t1.exon12;Parent=MS.gene94622.t1
chr2.4	CDS	14897504	14897636	14897504	ID=cds.MS.gene94622.t1;Parent=MS.gene94622.t1
chr2.4	exon	14897339	14897407	14897339	ID=MS.gene94622.t1.exon13;Parent=MS.gene94622.t1
chr2.4	CDS	14897339	14897407	14897339	ID=cds.MS.gene94622.t1;Parent=MS.gene94622.t1

Gene Sequence

>MS.gene94622

ATGGAGAAGCTTTATGTGTTTTTTGTCACTCTTGTATTCCTTGGTTTCCTATTTTATGAAGGAAAAGTTGGTGCTTTGAAGAGTGAAGATGGATTAGAAGAATGGGGATATGTGCAAGTTAGACCTAGTAAGCCAAATATATTAGAATTGTGTAATTTCAATTATTTTGTTTTCCAAACTTTTAAATTGATCGTTTCATGATATTGTAAATACTAGTTTGAATGTCATCGTGAGTTTAGCTTAGTTGATAAGGACAAGACATAGTATATTTAAGATCTGGGTTTGGACCGCAGCCACCACAAAAAAAAAAAACACTAGTTTGAACTCTTTCTATTTCACTCATTTTAGTATATTTTAGTATAATAATAAAATATTATTTATTTATTTAAAAAAAAAAATAGTTCAAATTAAAGTGTGTTTTAGGTCACACTTTAGGTTTCAATATATGTTGGGCCAATCATCGTGTTGGTAATCAATTTCTAAAATTATGGTGTTAACTTAAACAATAATTGACATTACCATTTATTTTAAATTGTGTTTTCCAAACTTTTAAATTGATCGGGTCATGATATTGTAAACCCTAGTTTGAATTCTTTGTATTTCACTCATAAGTATAATAATACATACAATATTATTATTTCTATAAAAAAATAGTTTGAATTAAAGTGTGTTTTAGGTCTCAATATGTATTAGGCCGATCATTAAGGTGTTACCGATTTTTAAAATTATGGCGTAATTAACTTAAACAATAGTTGACATTACCATTTATTTTGAATTGTGTTATCACAGAAGCACACATGTTCTGGTGGCTTTACAGAAGCCCCTACAGAGTGGAGGATCCCTCCAAACCATGGCCAATCATTCTCTGGTTGCAGGGAGGACCTGTAAGTATTATTTGAATTAAGCCATGATTTGAATTTTGAAAAAGAAAAATCCATGATTTGAATTTTGAACAAGAAAAATCCATGTTTTTATGTGTTTGTGAAATGAAGTTTCAAATCATTGAATTGAACAGGGTGGTTCAGGAGTTGGATTCGGAAATTTTATAGAGTTTGGCCCTCTTGATTCCAATTTGGAGCCAAGGAATTTCACTTGGTTGAGAAAAGCAGATTTGCTATTTGTGGTAACTCATTTTTTTTTTTTTGTTGCTGAATTTCACAAGTTAAGGTAACTAAAATTTGACAAAAATTACAATGACACCGTAATTACGGTTGCTCACCATAAATGTTTCTAAATTCACCGTCAATTCAAACATGCATCAACTAATTCATGTCACTCTTGTTTTACTTTCTTTTTTTTTTTTGCATACTTTGATTATTTTCTTTGAGGGTAAGGTTCTCAACTTCTCATCACTCATATTAAATGCATTTTTTGTTGTTAATTATAATATAGGACAATCCAGTTGGTACAGGGTTCAGTTTTGTTGAGGATTCAAAATTACTTGTCAAAACTGATGAGGAGGCAGCCACTGACTTGACTACACTATTAATCAAGATATTTAATAATGATCCAAGTCTACAAAAATGTAATTTGTTCATTTTTGGGGAGTCTTATGGTGGAAAATTTGCTGCTACTCTTGGATTATCAGCCCTCAATGCAATTAAAAAAGGAAGATTGAAGACTACACTTGGAGGTATTATGATTTCCTTGAAATTTTTCATCTATAAATGTGGTTAGTAATTTCTTCTTTTGATAGATTATGTATTATTTGAATATAGATTTAAACATATATTTATCTAGTTGGAGTCTGTCCAAACATACCGGTCCACGCCCCCCCAAGCACAAGTCCTGATAAAAGGCGAAATGGTTTGAAATGCATATGGCACTCCACAGCCCTCCAAACACGAGTCATAGGAAAGAGCGAAATGGTTTGAAATGCATACGGAGGTCCAATAAAATCTTTTTTAAAAAAACATTATAAGGAATATGAAAACAAACTACTAAAATGATGAAATATAAAAGACTATATAGAAATCATTTCAAAGCAAAAATGAAAAGAAAAAACTAAACTGAAAATTGGATATATCACAAATAAAAATCATATTAAAGTCATAAATGTGTTAAGAATAGTACTATATGGTATTTCTCGACTTTATGCTATTTTTATCCAAATTTAGGTGTGGTATTAGCAGACAGCTGGATCTCCCCAGAAGACTATGTGGTAAGAACATAAATAATTCTACCCCAAGATGTTCACAACATTAATTTGAGCTTTCACTTTTTTAGCATTTAATTTGACATTGCAGTTCTCATGGGGTCCTCTTTTGAAAGACATGTCAAGAATTGATGACAATGGACTGCAAAAATCAAACATGTATGTAACAAATGTTTTCAGTCCCATCTTCTAGTGTTAATCATGGTATTGTAGAATTAATGTACCAATTTCTCTCTATATTAAACTTGATTTTGAGAACAGTTTGGCAGAAAGGATCAAGCAACAACTTGAAGATGGTGAATTTGTTAATGCAACTTCTACATGGATTCAACTTGAATATGTAATTAGAAATAACAGCAACTTTGTGGTACTAACTTACATTTTTATTCTTAACATATTTTAAATTATTAATAATATACATAAACCACTAATGAATAATTAATTTCTTTATCTCATGTTTGTGTGTTTAAGGATTTCTATAATTTTATGGAGGACAACAATGGAAGTTATTCAGCAACCTCATCAACACTTGAGAAGAAATTATTCAAAGAAATATCAATGAGGCGTTACTCCAAGTATCTTACTTCATTGAAAAATTCTAATGCTGTTAATGGTGATCTAGACACCTTATTAAATGGTGTCACAAAGAAAAAATTGAAGATCATTCCCAAAAATATTACGTAAGTCTATATAACTTTGAGAAATAAAAATAAATGTACTTAGAATTCCAATGATTAAAACCCTAATCTCATGTTTCTTGCAATTGCTGACAGATGGTATGCGGAGTCAGTTTATGTGATTGAAATTTTTTTAGGTGAATTTATGAAACCAAGAATTGCTGAGGTGAGAAAATATTTAACTAATTAGAACAAGTTTGGAAAATATTCATCACGGTTTCTTGTAAAAATTATGCTTTGATTTTCTAAACTTAGGTGATTTCTATTTGATGTTTCTAGTTTGATTCTATCAGGTTGATGAGTTGCTAGCTCTTGGAGTCAATGTAACTGTGTACAATGGACAAGTAAGTGTTCTTTTTAAAAAACTCAGTATCCGGCCCAAATACCGATTGTTCTTTTCTATTAGTAATCTTGCAAAAAAATTTTTTTTTACTGTTATAGCTTGATCTCATTTGTGCAACCAAGGGAACTGAATCTTGGCTCAAGAAACTCAAGTAAGCAACCTCTCATTTATTTATTCACCCCTTCAAGTTTGGTGAAAGCAAACATCAATTTCATAATTGTATTTTTTTTATTACTTTTTGTGTTACTTGTGTAGATGGTCAGGCCTTCAAAATTTCTTGGGCAAAGACAGAACTCCTCTATATTGCGGAAGTGAAAGAAAGACCAAAGGCTTCTTTAAATCATATGAAAACCTAAACTTCTATTGGTTACTTGGTGCTGGCCATTTTGTAAGTCTAAATCCATCTTCTCAAACACTATATATATGCTTCTTTTTCAATATGGATATAGTTACCAAGTATAATTTACTTGGGTCATTGGTGTAGGTACCTATTGATCAGCCCTGCATAGCACTAAACATGGTGGGTGCAATTACACAGTCACCAGCGGTTTGA

Protein sequence

>MS.gene94622.t1

MEKLYVFFVTLVFLGFLFYEGKVGALKSEDGLEEWGYVQVRPKAHMFWWLYRSPYRVEDPSKPWPIILWLQGGPGGSGVGFGNFIEFGPLDSNLEPRNFTWLRKADLLFVDNPVGTGFSFVEDSKLLVKTDEEAATDLTTLLIKIFNNDPSLQKCNLFIFGESYGGKFAATLGLSALNAIKKGRLKTTLGGVVLADSWISPEDYVFSWGPLLKDMSRIDDNGLQKSNILAERIKQQLEDGEFVNATSTWIQLEYVIRNNSNFVDFYNFMEDNNGSYSATSSTLEKKLFKEISMRRYSKYLTSLKNSNAVNGDLDTLLNGVTKKKLKIIPKNITWYAESVYVIEIFLGEFMKPRIAEVDELLALGVNVTVYNGQLDLICATKGTESWLKKLKWSGLQNFLGKDRTPLYCGSERKTKGFFKSYENLNFYWLLGAGHFVPIDQPCIALNMVGAITQSPAV