AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05803

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05803.t1 MTR_2g018590 91.898 469 37 1 1 468 1 469 0.0 910
MS.gene05803.t1 MTR_2g018600 84.861 469 70 1 1 468 1 469 0.0 832
MS.gene05803.t1 MTR_2g018630 72.889 450 113 3 20 468 24 465 0.0 695
MS.gene05803.t1 MTR_3g100420 63.675 468 158 5 12 468 30 496 0.0 620
MS.gene05803.t1 MTR_4g021520 61.915 449 164 5 21 468 28 470 0.0 575
MS.gene05803.t1 MTR_4g021510 61.062 452 169 5 18 468 24 469 0.0 567
MS.gene05803.t1 MTR_4g021500 60.722 443 167 5 21 462 28 464 0.0 559
MS.gene05803.t1 MTR_4g021500 59.722 432 165 5 39 468 481 905 0.0 536
MS.gene05803.t1 MTR_4g021500 60.722 443 167 5 21 462 28 464 0.0 559
MS.gene05803.t1 MTR_4g021500 58.333 432 153 6 39 468 481 887 3.55e-173 509
MS.gene05803.t1 MTR_4g021535 57.174 460 190 5 10 468 13 466 0.0 551
MS.gene05803.t1 MTR_4g021490 58.797 449 178 5 21 468 28 470 0.0 551
MS.gene05803.t1 MTR_4g021530 56.957 460 191 6 10 468 17 470 0.0 538
MS.gene05803.t1 MTR_2g018630 69.755 367 102 3 103 468 2 360 0.0 538
MS.gene05803.t1 MTR_4g021520 61.179 407 151 5 21 426 28 428 5.07e-179 508
MS.gene05803.t1 MTR_4g021535 50.435 460 174 6 10 468 13 419 3.80e-160 459
MS.gene05803.t1 MTR_3g045770 44.227 459 233 5 11 464 10 450 3.96e-137 402
MS.gene05803.t1 MTR_2g009200 42.198 455 225 7 16 463 11 434 3.08e-124 369
MS.gene05803.t1 MTR_8g011350 39.437 497 262 8 1 464 1 491 1.71e-123 369
MS.gene05803.t1 MTR_7g051430 40.041 487 254 8 11 464 10 491 2.73e-122 366
MS.gene05803.t1 MTR_7g080180 39.919 496 255 10 11 468 8 498 2.47e-121 363
MS.gene05803.t1 MTR_8g011350 38.819 474 251 8 1 441 1 468 9.02e-112 338
MS.gene05803.t1 MTR_7g080180 38.546 454 236 10 11 426 8 456 8.96e-101 310
MS.gene05803.t1 MTR_7g051430 37.805 410 217 8 11 387 10 414 1.72e-85 268
MS.gene05803.t1 MTR_4g075150 32.623 469 286 15 13 468 9 460 3.03e-67 223
MS.gene05803.t1 MTR_4g075150 32.265 468 291 14 13 468 9 462 4.86e-67 222
MS.gene05803.t1 MTR_4g122950 30.000 470 302 16 13 468 20 476 2.38e-58 200
MS.gene05803.t1 MTR_8g099930 31.646 474 272 19 13 468 22 461 2.58e-58 199
MS.gene05803.t1 MTR_4g122960 30.213 470 299 17 13 468 20 474 2.72e-58 199
MS.gene05803.t1 MTR_2g012860 30.719 459 285 15 24 468 33 472 1.19e-57 197
MS.gene05803.t1 MTR_2g012850 30.112 445 278 14 32 463 35 459 1.15e-56 195
MS.gene05803.t1 MTR_7g013790 29.688 448 277 13 36 468 39 463 4.14e-56 193
MS.gene05803.t1 MTR_1g021010 32.877 438 254 15 33 463 76 480 6.49e-56 193
MS.gene05803.t1 MTR_2g062980 28.810 479 308 14 2 468 4 461 7.77e-56 193
MS.gene05803.t1 MTR_2g062990 28.392 479 310 15 2 468 4 461 9.08e-56 192
MS.gene05803.t1 MTR_3g434850 29.930 431 266 14 50 468 47 453 1.12e-55 192
MS.gene05803.t1 MTR_2g063000 29.306 447 286 12 31 468 37 462 1.59e-55 192
MS.gene05803.t1 MTR_3g079590 30.415 434 266 12 36 463 81 484 3.35e-55 192
MS.gene05803.t1 MTR_5g072570 28.361 476 301 16 12 468 6 460 4.89e-55 191
MS.gene05803.t1 MTR_1g095560 30.335 478 291 17 9 468 2 455 2.39e-54 188
MS.gene05803.t1 MTR_1g095540 29.325 474 295 16 12 468 5 455 2.82e-54 188
MS.gene05803.t1 MTR_3g498755 28.998 469 283 14 22 468 4 444 2.90e-53 185
MS.gene05803.t1 MTR_2g081620 29.844 449 269 16 36 468 44 462 3.73e-53 186
MS.gene05803.t1 MTR_4g089155 29.478 441 288 14 32 463 53 479 1.38e-52 184
MS.gene05803.t1 MTR_3g079570 29.630 432 271 12 36 463 82 484 2.12e-52 184
MS.gene05803.t1 MTR_3g034290 28.571 441 284 12 33 468 75 489 2.40e-52 184
MS.gene05803.t1 MTR_3g079560 29.680 438 270 13 33 463 78 484 3.67e-52 183
MS.gene05803.t1 MTR_1g020090 32.500 440 250 14 33 463 90 491 3.83e-52 184
MS.gene05803.t1 MTR_0003s0560 28.989 445 275 13 33 468 74 486 7.49e-52 182
MS.gene05803.t1 MTR_2g449850 29.330 433 262 14 51 467 56 460 1.09e-51 181
MS.gene05803.t1 MTR_3g090130 29.556 450 282 14 32 468 28 455 1.52e-51 181
MS.gene05803.t1 MTR_3g079600 29.062 437 277 13 33 463 76 485 7.83e-50 177
MS.gene05803.t1 MTR_7g021130 29.157 439 264 13 49 468 46 456 1.07e-49 176
MS.gene05803.t1 MTR_3g090150 28.352 455 282 16 32 468 34 462 4.55e-49 175
MS.gene05803.t1 MTR_1g021020 29.864 442 261 15 33 463 78 481 2.73e-48 173
MS.gene05803.t1 MTR_3g079620 29.186 442 269 14 36 463 81 492 3.92e-48 173
MS.gene05803.t1 MTR_1g095560 28.431 510 291 18 9 468 2 487 4.85e-48 172
MS.gene05803.t1 MTR_3g079630 28.764 445 267 15 36 463 81 492 4.14e-47 170
MS.gene05803.t1 MTR_2g063000 28.153 444 264 12 31 468 37 431 1.23e-46 167
MS.gene05803.t1 MTR_2g095750 27.556 450 275 10 36 468 85 500 1.45e-46 169
MS.gene05803.t1 MTR_1g040370 28.074 431 237 14 49 468 46 414 2.43e-46 166
MS.gene05803.t1 MTR_4g057585 28.111 434 252 11 49 463 98 490 8.55e-46 166
MS.gene05803.t1 MTR_4g057595 28.111 434 252 11 49 463 99 491 9.01e-46 166
MS.gene05803.t1 MTR_4g057600 27.083 432 265 10 49 466 97 492 2.81e-45 165
MS.gene05803.t1 MTR_1g050545 27.126 435 254 10 49 462 85 477 2.24e-44 162
MS.gene05803.t1 MTR_2g095730 29.211 469 257 19 30 466 70 495 2.37e-44 162
MS.gene05803.t1 MTR_1g094155 27.126 435 253 13 49 462 92 483 9.80e-42 155
MS.gene05803.t1 MTR_4g057570 26.185 443 257 13 50 466 92 490 1.72e-41 154
MS.gene05803.t1 MTR_5g097180 25.170 441 265 14 48 468 42 437 2.18e-39 148
MS.gene05803.t1 MTR_1g102130 26.637 443 242 16 49 468 107 489 1.13e-37 144
MS.gene05803.t1 MTR_2g018650 54.762 126 37 3 316 432 8 122 2.35e-36 130
MS.gene05803.t1 MTR_3g034290 31.250 272 175 7 33 301 75 337 2.43e-36 139
MS.gene05803.t1 MTR_0003s0560 30.662 287 184 9 33 316 74 348 5.55e-35 134
MS.gene05803.t1 MTR_4g084710 24.887 442 263 14 49 468 45 439 1.02e-32 129
MS.gene05803.t1 MTR_2g095710 25.951 447 275 14 36 468 79 483 2.06e-30 123
MS.gene05803.t1 MTR_4g056520 22.573 443 287 17 48 460 34 450 5.34e-13 71.2
MS.gene05803.t1 MTR_2g084530 23.608 449 269 18 48 458 34 446 1.95e-11 66.2
MS.gene05803.t1 MTR_0075s0010 36.957 92 44 3 62 146 35 119 5.04e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05803.t1 AT2G22920 53.947 456 174 6 16 468 13 435 6.66e-175 498
MS.gene05803.t1 AT5G09640 53.118 465 205 9 12 468 6 465 1.81e-174 498
MS.gene05803.t1 AT1G73300 55.730 445 168 4 25 468 25 441 1.90e-173 494
MS.gene05803.t1 AT1G73300 55.730 445 168 4 25 468 25 441 1.90e-173 494
MS.gene05803.t1 AT1G33540 54.422 441 197 4 30 468 26 464 3.63e-173 494
MS.gene05803.t1 AT2G22970 54.545 440 171 4 30 468 22 433 4.20e-173 493
MS.gene05803.t1 AT2G22980 55.856 444 161 6 27 468 20 430 1.70e-172 491
MS.gene05803.t1 AT5G36180 56.180 445 166 4 25 468 25 441 2.17e-172 491
MS.gene05803.t1 AT1G73280 52.452 469 191 5 4 468 1 441 1.90e-171 489
MS.gene05803.t1 AT2G23000 54.545 440 175 4 30 468 22 437 9.07e-170 485
MS.gene05803.t1 AT2G23000 54.545 440 175 4 30 468 22 437 9.07e-170 485
MS.gene05803.t1 AT1G73310 54.831 445 172 4 25 468 25 441 2.75e-169 484
MS.gene05803.t1 AT3G10450 52.809 445 181 4 25 468 21 437 2.95e-169 483
MS.gene05803.t1 AT2G22990 54.318 440 174 5 30 468 20 433 3.75e-168 481
MS.gene05803.t1 AT1G73290 53.483 445 176 5 25 468 24 438 4.23e-168 481
MS.gene05803.t1 AT1G73270 54.545 440 171 3 30 468 30 441 1.49e-167 480
MS.gene05803.t1 AT1G73270 54.545 440 171 3 30 468 30 441 1.63e-167 479
MS.gene05803.t1 AT2G23010 52.391 460 190 5 10 468 6 437 5.13e-167 478
MS.gene05803.t1 AT2G23010 51.957 460 192 5 10 468 6 437 2.23e-165 474
MS.gene05803.t1 AT2G22970 54.327 416 162 3 30 444 22 410 5.09e-163 468
MS.gene05803.t1 AT2G22970 54.348 414 161 3 30 442 22 408 7.14e-163 466
MS.gene05803.t1 AT1G33540 53.810 420 190 4 51 468 4 421 3.23e-162 465
MS.gene05803.t1 AT2G22980 55.502 418 152 5 27 442 20 405 3.16e-161 462
MS.gene05803.t1 AT2G22980 55.502 418 152 5 27 442 20 405 1.02e-160 462
MS.gene05803.t1 AT3G12203 50.223 448 193 3 22 468 19 437 4.53e-160 460
MS.gene05803.t1 AT3G12203 50.909 440 186 3 30 468 6 416 1.90e-159 458
MS.gene05803.t1 AT2G22990 52.727 440 172 6 30 468 20 424 4.01e-159 457
MS.gene05803.t1 AT3G12240 51.121 446 189 4 24 468 19 436 1.09e-158 457
MS.gene05803.t1 AT3G10450 52.267 419 172 3 25 442 21 412 1.49e-156 450
MS.gene05803.t1 AT3G10450 51.790 419 173 4 51 468 4 394 3.53e-156 449
MS.gene05803.t1 AT3G12230 50.564 443 190 4 27 468 21 435 5.09e-156 450
MS.gene05803.t1 AT2G22990 53.865 414 165 4 30 442 20 408 1.23e-155 448
MS.gene05803.t1 AT2G22990 53.589 418 168 4 30 446 20 412 1.72e-155 449
MS.gene05803.t1 AT2G22920 52.289 415 163 5 16 427 13 395 4.99e-153 441
MS.gene05803.t1 AT3G12220 49.661 443 194 3 27 468 21 435 3.75e-151 437
MS.gene05803.t1 AT1G73310 53.676 408 161 3 25 431 25 405 8.15e-151 436
MS.gene05803.t1 AT1G73290 52.723 404 161 4 25 427 24 398 1.10e-147 427
MS.gene05803.t1 AT3G10450 51.145 393 164 3 51 442 4 369 1.75e-143 416
MS.gene05803.t1 AT3G12203 49.265 408 177 3 62 468 1 379 6.39e-140 407
MS.gene05803.t1 AT3G12220 48.210 419 188 3 51 468 15 405 5.63e-137 400
MS.gene05803.t1 AT4G12910 40.909 484 254 7 10 463 11 492 2.42e-127 379
MS.gene05803.t1 AT1G73280 49.742 388 164 4 4 387 1 361 3.78e-125 369
MS.gene05803.t1 AT5G36180 52.044 367 147 4 103 468 1 339 4.54e-125 367
MS.gene05803.t1 AT5G36180 52.044 367 147 4 103 468 1 339 4.54e-125 367
MS.gene05803.t1 AT1G73280 50.409 367 153 4 103 468 1 339 9.24e-124 364
MS.gene05803.t1 AT2G22980 52.055 365 139 6 27 387 20 352 1.55e-121 359
MS.gene05803.t1 AT3G25420 38.134 493 267 9 10 468 6 494 6.81e-121 362
MS.gene05803.t1 AT2G22990 51.014 345 142 5 125 468 1 319 4.65e-116 343
MS.gene05803.t1 AT5G36180 51.462 342 138 3 103 443 1 315 7.85e-115 340
MS.gene05803.t1 AT2G22990 50.435 345 144 5 125 468 1 319 1.33e-114 340
MS.gene05803.t1 AT1G73280 50.585 342 140 4 128 468 1 314 2.49e-114 339
MS.gene05803.t1 AT2G22970 51.097 319 129 2 30 348 22 313 9.73e-113 335
MS.gene05803.t1 AT2G22920 49.401 334 136 4 16 348 13 314 7.13e-112 333
MS.gene05803.t1 AT4G12910 39.597 447 238 7 10 426 11 455 3.86e-108 328
MS.gene05803.t1 AT5G36180 48.851 348 148 4 103 447 1 321 4.38e-104 314
MS.gene05803.t1 AT2G22980 50.313 320 125 4 125 442 1 288 4.28e-103 310
MS.gene05803.t1 AT1G73290 56.705 261 110 2 30 289 29 287 1.89e-102 308
MS.gene05803.t1 AT1G73310 48.632 329 141 3 104 431 2 303 2.06e-102 309
MS.gene05803.t1 AT2G22920 51.408 284 107 5 188 468 1 256 6.88e-94 285
MS.gene05803.t1 AT3G12220 55.319 235 104 1 27 260 21 255 1.26e-82 260
MS.gene05803.t1 AT3G12220 53.793 145 66 1 324 468 240 383 3.11e-48 170
MS.gene05803.t1 AT3G52010 31.250 448 269 11 32 468 64 483 2.09e-66 221
MS.gene05803.t1 AT5G23210 31.635 471 283 16 23 468 38 494 7.68e-66 220
MS.gene05803.t1 AT3G52000 30.269 446 272 10 32 468 63 478 3.90e-65 218
MS.gene05803.t1 AT2G05850 30.243 453 268 16 32 468 63 483 3.32e-62 210
MS.gene05803.t1 AT4G30610 32.747 455 261 17 33 468 33 461 3.51e-61 207
MS.gene05803.t1 AT4G15100 29.424 486 309 13 1 463 1 475 6.95e-61 206
MS.gene05803.t1 AT3G52020 31.929 451 261 13 32 468 79 497 1.31e-60 206
MS.gene05803.t1 AT3G63470 30.317 442 277 10 32 468 81 496 1.61e-60 206
MS.gene05803.t1 AT2G22960 65.441 136 46 1 333 468 50 184 2.85e-59 193
MS.gene05803.t1 AT4G30810 27.706 462 308 12 15 468 18 461 3.88e-58 199
MS.gene05803.t1 AT3G17180 29.252 441 270 14 49 468 55 474 4.37e-57 196
MS.gene05803.t1 AT1G11080 32.294 449 274 15 32 464 50 484 3.92e-56 194
MS.gene05803.t1 AT1G11080 32.294 449 274 15 32 464 50 484 4.04e-56 194
MS.gene05803.t1 AT5G08260 29.712 451 289 16 32 468 39 475 5.06e-56 193
MS.gene05803.t1 AT2G24010 30.180 444 280 12 32 468 30 450 6.14e-56 192
MS.gene05803.t1 AT1G61130 30.717 446 275 15 32 464 31 455 8.49e-56 192
MS.gene05803.t1 AT2G24000 30.045 446 286 12 33 468 31 460 2.97e-55 191
MS.gene05803.t1 AT2G35780 29.888 445 273 15 36 468 33 450 1.31e-54 189
MS.gene05803.t1 AT1G43780 29.646 452 284 14 32 468 37 469 1.78e-53 187
MS.gene05803.t1 AT5G23210 30.679 427 258 15 23 424 38 451 1.90e-53 186
MS.gene05803.t1 AT1G28110 29.556 450 279 13 33 468 32 457 4.23e-53 185
MS.gene05803.t1 AT1G28110 29.556 450 279 13 33 468 32 457 4.23e-53 185
MS.gene05803.t1 AT2G35770 29.754 447 270 12 36 468 41 457 2.77e-52 183
MS.gene05803.t1 AT2G12480 28.667 450 284 14 32 468 31 456 8.57e-52 182
MS.gene05803.t1 AT2G33530 29.778 450 274 16 36 468 37 461 1.45e-51 181
MS.gene05803.t1 AT3G02110 29.730 444 287 13 36 468 41 470 6.56e-51 179
MS.gene05803.t1 AT2G24010 28.976 459 281 13 32 468 30 465 8.60e-51 179
MS.gene05803.t1 AT5G23210 30.370 405 248 14 85 468 8 399 2.06e-50 177
MS.gene05803.t1 AT3G07990 29.268 451 270 16 36 468 37 456 3.11e-50 177
MS.gene05803.t1 AT5G22980 29.930 431 253 14 49 466 100 494 5.24e-50 178
MS.gene05803.t1 AT5G23210 30.198 404 248 14 86 468 8 398 1.17e-49 174
MS.gene05803.t1 AT5G42240 28.947 456 281 16 32 468 32 463 4.10e-49 175
MS.gene05803.t1 AT2G12480 27.617 449 266 13 32 468 31 432 9.22e-49 173
MS.gene05803.t1 AT2G12480 27.394 449 265 13 32 468 31 430 1.13e-47 170
MS.gene05803.t1 AT5G42230 28.667 450 290 15 32 468 28 459 7.46e-47 169
MS.gene05803.t1 AT5G42230 28.667 450 290 15 32 468 28 459 7.46e-47 169
MS.gene05803.t1 AT3G10410 28.302 424 263 11 49 463 101 492 1.57e-46 169
MS.gene05803.t1 AT2G12480 27.333 450 265 14 32 468 31 431 3.74e-46 166
MS.gene05803.t1 AT3G17180 28.392 398 247 13 49 427 55 433 4.80e-46 166
MS.gene05803.t1 AT3G45010 27.854 438 263 12 45 466 99 499 5.26e-46 167
MS.gene05803.t1 AT2G24010 27.479 484 281 14 32 468 1 461 1.13e-45 165
MS.gene05803.t1 AT5G23210 28.889 360 223 13 86 424 8 355 4.02e-37 140
MS.gene05803.t1 AT1G15000 24.598 435 270 15 48 468 36 426 1.03e-32 129
MS.gene05803.t1 AT2G24010 29.462 353 207 11 32 364 1 331 1.23e-32 127
MS.gene05803.t1 AT3G56540 37.580 157 91 3 32 187 79 229 1.34e-29 117
MS.gene05803.t1 AT5G22960 30.303 165 90 2 48 212 35 174 2.14e-25 103
MS.gene05803.t1 AT2G24000 25.862 290 198 7 186 468 118 397 2.66e-21 96.3
MS.gene05803.t1 AT2G27920 23.490 447 272 22 50 458 37 451 2.56e-13 72.4

Find 92 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATTTCCTTATGGGTTTC+TGG -0.005262 2.3:-67969134 MS.gene05803:CDS
TTTGGAGCATAAAACATTTC+TGG 0.147168 2.3:+67969522 None:intergenic
ATTGAATGAATTGATATCTT+TGG 0.232350 2.3:+67969504 None:intergenic
TTGTTTGTAGGAAATGAAAA+AGG 0.248089 2.3:-67970086 MS.gene05803:intron
ACAAAGTTTAGTTCAACTTA+TGG 0.250544 2.3:-67971789 MS.gene05803:CDS
TGACATGACAATTCCATTCT+TGG 0.262363 2.3:-67968702 MS.gene05803:CDS
GAGTTATTCATATTTCCTTA+TGG 0.267750 2.3:-67969142 MS.gene05803:CDS
ATTTCATTTGATTGAAACTT+TGG 0.310987 2.3:+67970271 None:intergenic
AGGTCCACTTGAGTTTAAAA+AGG 0.317116 2.3:-67970766 MS.gene05803:CDS
TGTGGCTTCAAGATCAAATT+AGG 0.326932 2.3:+67970723 None:intergenic
ATATTTCCTTATGGGTTTCT+GGG 0.329687 2.3:-67969133 MS.gene05803:CDS
TCAAATGAAATTTACATTGC+TGG 0.330344 2.3:-67970259 MS.gene05803:CDS
ACATGATAATCATAGCTGTT+AGG 0.338091 2.3:+67968861 None:intergenic
TGATCTTGAAGCCACATTCA+TGG 0.345897 2.3:-67970716 MS.gene05803:CDS
CAAAGTTTAGTTCAACTTAT+GGG 0.357058 2.3:-67971788 MS.gene05803:CDS
AGGATGATCCTCTCATACTA+TGG 0.360528 2.3:-67971495 MS.gene05803:CDS
AAGCGTTACTCGACAACTTA+AGG 0.360759 2.3:+67969261 None:intergenic
AGTTATTCATATTTCCTTAT+GGG 0.366957 2.3:-67969141 MS.gene05803:CDS
ACTATTCCATTGTTGATGAT+TGG 0.372894 2.3:-67968652 MS.gene05803:CDS
CCTGGTTGCTCTGCTTTGTC+TGG 0.374838 2.3:-67971460 MS.gene05803:CDS
TCTATAGTGAAGTTTCTTCC+TGG 0.395633 2.3:-67971702 MS.gene05803:CDS
CATACAAATGACCAAGTTGC+AGG 0.404512 2.3:-67968620 MS.gene05803:CDS
ATACTCTGGTATTGCTGTTC+CGG 0.407889 2.3:-67970230 MS.gene05803:CDS
TTTCTTCAGGGATACTTGTT+GGG 0.407975 2.3:-67969758 MS.gene05803:intron
TGCAGGGAAGTATTGGAAAA+TGG 0.419352 2.3:-67968923 MS.gene05803:intron
CATTCTTTAGGCTTGAACTC+TGG 0.424333 2.3:+67968341 None:intergenic
TCTTATGCCACTACAGAATC+TGG 0.426608 2.3:-67970519 MS.gene05803:CDS
CATGATAATCATAGCTGTTA+GGG 0.426927 2.3:+67968862 None:intergenic
AGTCCCATTCCATGAGCAAA+TGG 0.428128 2.3:+67969695 None:intergenic
ATCCCATTTGCTCATGGAAT+GGG 0.431219 2.3:-67969698 MS.gene05803:CDS
ATATTTGTTGTTAAGGTATG+TGG 0.432299 2.3:-67971581 MS.gene05803:intron
GCAATTTGAGATTTGTATTG+AGG 0.443024 2.3:+67969287 None:intergenic
TTGTTAAGGTATGTGGGAGT+TGG 0.444634 2.3:-67971574 MS.gene05803:intron
AGTATGAGAGGATCATCCTT+TGG 0.453644 2.3:+67971499 None:intergenic
CAGGGATACTTGTTGGGCAA+TGG 0.453796 2.3:-67969752 MS.gene05803:CDS
CATGTAAATCTCAGTAAAAG+AGG 0.455236 2.3:-67968843 MS.gene05803:CDS
CATGTGTTGCAGGGAAGTAT+TGG 0.455548 2.3:-67968930 MS.gene05803:intron
AATGTTTCGACATGTTTAGT+AGG 0.458153 2.3:-67968322 MS.gene05803:CDS
TAAGAGAAGCCTGTGGAAAC+CGG 0.462321 2.3:+67970537 None:intergenic
CATGCTTGTGTTGCCAAGAA+TGG 0.464724 2.3:+67968689 None:intergenic
CATTATATTTGTAGATTCGC+CGG 0.473076 2.3:-67970556 MS.gene05803:CDS
GGAAGGGGTCCTTCGAATCC+AGG 0.474859 2.3:+67971684 None:intergenic
AACATGTCGAAACATTCTTT+AGG 0.475912 2.3:+67968329 None:intergenic
CTATGGCTCACTGGTGGACC+TGG 0.476423 2.3:-67971478 MS.gene05803:CDS
GAGATTTGTATTGAGGAACT+TGG 0.479830 2.3:+67969294 None:intergenic
AGCATTATGACGTATATTGT+AGG 0.481619 2.3:+67968890 None:intergenic
AACCCGGTTTCAAGCACAAA+AGG 0.485577 2.3:+67971663 None:intergenic
CGAACCATTGATAAATCTCC+AGG 0.488461 2.3:-67970060 MS.gene05803:intron
ATTGCTGGTGATTCATACTC+TGG 0.492272 2.3:-67970244 MS.gene05803:CDS
TATTTGTTGTTAAGGTATGT+GGG 0.503693 2.3:-67971580 MS.gene05803:intron
GTAGATTCGCCGGTTTCCAC+AGG 0.511485 2.3:-67970546 MS.gene05803:CDS
TGCTTTCAAGTTGCAACATG+TGG 0.513785 2.3:-67971726 MS.gene05803:CDS
TGATCAAAATTTCAGGGTGG+AGG 0.517860 2.3:-67968374 MS.gene05803:intron
CTAACCTGGAGATTTATCAA+TGG 0.519088 2.3:+67970056 None:intergenic
AATCCCATTTGCTCATGGAA+TGG 0.520162 2.3:-67969699 MS.gene05803:CDS
CCTCTCATACTATGGCTCAC+TGG 0.524416 2.3:-67971487 MS.gene05803:CDS
GGTTTCAAGCACAAAAGGAA+GGG 0.526633 2.3:+67971668 None:intergenic
AGCGTTACTCGACAACTTAA+GGG 0.526961 2.3:+67969262 None:intergenic
CATTAGCCCAGAAACCCATA+AGG 0.530053 2.3:+67969127 None:intergenic
TATGCAATCCCATTTGCTCA+TGG 0.530149 2.3:-67969704 MS.gene05803:CDS
CGGTTTCAAGCACAAAAGGA+AGG 0.531662 2.3:+67971667 None:intergenic
CGAACCTTTGTCCATGAATG+TGG 0.535010 2.3:+67970705 None:intergenic
AAGTTTCTTCCTGGATTCGA+AGG 0.543135 2.3:-67971693 MS.gene05803:CDS
CAATGGTGTAACAACTAGAA+AGG 0.545007 2.3:-67969735 MS.gene05803:CDS
TGTGGGAGTTGGTGAGAAAG+AGG 0.550305 2.3:-67971563 MS.gene05803:CDS
CATTCTTGGCAACACAAGCA+TGG 0.552262 2.3:-67968688 MS.gene05803:CDS
GTCCCATTCCATGAGCAAAT+GGG 0.553573 2.3:+67969696 None:intergenic
GTTTAGTTCAACTTATGGGA+AGG 0.563304 2.3:-67971784 MS.gene05803:CDS
CTCATACTATGGCTCACTGG+TGG 0.567761 2.3:-67971484 MS.gene05803:CDS
GTTTCAAGCACAAAAGGAAG+GGG 0.572128 2.3:+67971669 None:intergenic
GTTTCGACATGTTTAGTAGG+TGG 0.572959 2.3:-67968319 MS.gene05803:CDS
CCAGACAAAGCAGAGCAACC+AGG 0.587330 2.3:+67971460 None:intergenic
ATTGTTCACAAAATTATACA+AGG 0.587653 2.3:-67970205 MS.gene05803:intron
CCAGTGAGCCATAGTATGAG+AGG 0.603687 2.3:+67971487 None:intergenic
CGTCACGCCACTCACATAGC+GGG 0.606581 2.3:+67969362 None:intergenic
CAAAGCACTACACATACGTA+AGG 0.618643 2.3:-67969091 MS.gene05803:intron
GATGACATTTGCAACTGTGA+AGG 0.622581 2.3:-67968466 MS.gene05803:intron
TTCTTAATTAGAGATCTCCT+CGG 0.626664 2.3:+67969325 None:intergenic
GCTTCAAGATCAAATTAGGT+AGG 0.628776 2.3:+67970727 None:intergenic
CTGTCTCCAATCATCAACAA+TGG 0.629618 2.3:+67968646 None:intergenic
GAAGCCACATTCATGGACAA+AGG 0.632479 2.3:-67970709 MS.gene05803:intron
ATACAAATGACCAAGTTGCA+GGG 0.634663 2.3:-67968619 MS.gene05803:intron
CTTAGCACCAGATTCTGTAG+TGG 0.636413 2.3:+67970512 None:intergenic
AGATATCAATTCATTCAATG+AGG 0.653803 2.3:-67969500 MS.gene05803:intron
TGAATCAGAGAAGAATCCAA+AGG 0.659518 2.3:-67971515 MS.gene05803:CDS
CATATCTGATGAACTATACG+AGG 0.661127 2.3:-67969672 MS.gene05803:intron
TTGTTGATGATTGGAGACAG+TGG 0.718131 2.3:-67968643 MS.gene05803:CDS
TCGTCACGCCACTCACATAG+CGG 0.718285 2.3:+67969361 None:intergenic
GTTACTCGACAACTTAAGGG+TGG 0.723170 2.3:+67969265 None:intergenic
AGTGGCATAAGAGAAGCCTG+TGG 0.736747 2.3:+67970530 None:intergenic
TGTGCAAGTATAGATACACA+AGG 0.750084 2.3:-67968503 MS.gene05803:intron
ACGATACTGAAAATTCTCCG+AGG 0.753318 2.3:-67969342 MS.gene05803:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAAAATAGTATAATAAT+TGG - chr2.3:67968976-67968995 MS.gene05803:intron 10.0%
!! TTATTAACAAAAATTTCAAT+TGG + chr2.3:67968725-67968744 None:intergenic 10.0%
!!! AAGTTTTTTTTAATCTATTA+TGG - chr2.3:67968465-67968484 MS.gene05803:intron 10.0%
!! AAGACATTCAAATTAAAATT+AGG - chr2.3:67969197-67969216 MS.gene05803:intron 15.0%
!! GTTAATAAAGAAAAATGAAT+GGG - chr2.3:67968737-67968756 MS.gene05803:intron 15.0%
!! TAAAAATTTATGGAAAACAA+AGG + chr2.3:67971091-67971110 None:intergenic 15.0%
!! TATAATTTCTTATGGAAAAT+TGG - chr2.3:67971336-67971355 MS.gene05803:intron 15.0%
!! TGTTAATAAAGAAAAATGAA+TGG - chr2.3:67968736-67968755 MS.gene05803:intron 15.0%
!!! TTATTTTTAACACATGATTA+TGG + chr2.3:67971668-67971687 None:intergenic 15.0%
!!! TTTTATATTTACAATAACAG+TGG - chr2.3:67971359-67971378 MS.gene05803:intron 15.0%
!! AATGATTGATCAAAATTTCA+GGG - chr2.3:67971710-67971729 MS.gene05803:CDS 20.0%
!! AGAGAAATATTAATTCAACT+TGG + chr2.3:67971555-67971574 None:intergenic 20.0%
!! AGTTATTCATATTTCCTTAT+GGG - chr2.3:67970949-67970968 MS.gene05803:intron 20.0%
!! ATTGAATGAATTGATATCTT+TGG + chr2.3:67970589-67970608 None:intergenic 20.0%
!! ATTGTTCACAAAATTATACA+AGG - chr2.3:67969885-67969904 MS.gene05803:intron 20.0%
!! ATTTCATTTGATTGAAACTT+TGG + chr2.3:67969822-67969841 None:intergenic 20.0%
!! CTATAAATAAATCTTTGTAG+TGG - chr2.3:67969784-67969803 MS.gene05803:intron 20.0%
!! TATAATTATAATGCAGCAAA+TGG + chr2.3:67970870-67970889 None:intergenic 20.0%
!! TCCTTAAAAAATCATATGTT+TGG + chr2.3:67969627-67969646 None:intergenic 20.0%
!! TGAATGTGGATAAAAATTTA+TGG + chr2.3:67971101-67971120 None:intergenic 20.0%
!!! AACATATGATTTTTTAAGGA+AGG - chr2.3:67969627-67969646 MS.gene05803:intron 20.0%
!!! AATATGAATAACTCTAAAAG+TGG + chr2.3:67970942-67970961 None:intergenic 20.0%
!!! ACCAAACATATGATTTTTTA+AGG - chr2.3:67969623-67969642 MS.gene05803:intron 20.0%
!!! ACTAGTCTTATAATTTCTTA+TGG - chr2.3:67971328-67971347 MS.gene05803:intron 20.0%
!!! ATAATTTTGTGAACAATTAC+CGG + chr2.3:67969882-67969901 None:intergenic 20.0%
!!! ATAGCATAATTTTCGTTATT+TGG + chr2.3:67969768-67969787 None:intergenic 20.0%
!!! ATGAAATGTTATTTTCTTCA+GGG - chr2.3:67970320-67970339 MS.gene05803:intron 20.0%
!!! ATTTATAAGATTTTTTCCCT+TGG - chr2.3:67969500-67969519 MS.gene05803:intron 20.0%
!!! CATATTCATATTTGTTGTTA+AGG - chr2.3:67968502-67968521 MS.gene05803:intron 20.0%
!!! CATTAAGTTGATCAAAAAAT+CGG + chr2.3:67970198-67970217 None:intergenic 20.0%
!!! TATGAAATGTTATTTTCTTC+AGG - chr2.3:67970319-67970338 MS.gene05803:intron 20.0%
!!! TTAACTTTTGCCAAATATAT+CGG + chr2.3:67968810-67968829 None:intergenic 20.0%
! AAATAAATCTTTGTAGTGGT+TGG - chr2.3:67969788-67969807 MS.gene05803:intron 25.0%
! AATTGAACTGTAAATGTTAC+AGG - chr2.3:67969304-67969323 MS.gene05803:CDS 25.0%
! ACTTGGTCATAAATTTATCA+TGG + chr2.3:67971538-67971557 None:intergenic 25.0%
! AGATATCAATTCATTCAATG+AGG - chr2.3:67970590-67970609 MS.gene05803:intron 25.0%
! ATCAATTAGTTAGCTATGTA+TGG + chr2.3:67970489-67970508 None:intergenic 25.0%
! GAATGATTGATCAAAATTTC+AGG - chr2.3:67971709-67971728 MS.gene05803:CDS 25.0%
! GAGTTATTCATATTTCCTTA+TGG - chr2.3:67970948-67970967 MS.gene05803:intron 25.0%
! TAGATATAATTCATCTCTGT+TGG - chr2.3:67969173-67969192 MS.gene05803:intron 25.0%
! TCAAATGAAATTTACATTGC+TGG - chr2.3:67969831-67969850 MS.gene05803:intron 25.0%
! TGTAAAGAAATTTACTGATG+TGG - chr2.3:67969261-67969280 MS.gene05803:intron 25.0%
! TGTTTGTAGGAAATGAAAAA+GGG - chr2.3:67970005-67970024 MS.gene05803:intron 25.0%
! TTAAAAAGGAGGAATACAAT+GGG - chr2.3:67969338-67969357 MS.gene05803:CDS 25.0%
! TTGTTAGTACTTATGTCAAT+CGG - chr2.3:67969738-67969757 MS.gene05803:CDS 25.0%
! TTGTTTGTAGGAAATGAAAA+AGG - chr2.3:67970004-67970023 MS.gene05803:intron 25.0%
! TTTAAAAAGGAGGAATACAA+TGG - chr2.3:67969337-67969356 MS.gene05803:CDS 25.0%
!! ACAAAGTTTAGTTCAACTTA+TGG - chr2.3:67968301-67968320 MS.gene05803:CDS 25.0%
!! CAAAGTTTAGTTCAACTTAT+GGG - chr2.3:67968302-67968321 MS.gene05803:CDS 25.0%
!! CTTTTCAAATTGTTCACTTT+TGG - chr2.3:67968684-67968703 MS.gene05803:CDS 25.0%
!!! ATATTTGTTGTTAAGGTATG+TGG - chr2.3:67968509-67968528 MS.gene05803:intron 25.0%
!!! TATTTGTTGTTAAGGTATGT+GGG - chr2.3:67968510-67968529 MS.gene05803:intron 25.0%
AAATCAGGTGACAAAAAATG+TGG + chr2.3:67969420-67969439 None:intergenic 30.0%
AACATGTCGAAACATTCTTT+AGG + chr2.3:67971764-67971783 None:intergenic 30.0%
AATGTTTCGACATGTTTAGT+AGG - chr2.3:67971768-67971787 MS.gene05803:CDS 30.0%
AATTAGTTAGCTATGTATGG+AGG + chr2.3:67970486-67970505 None:intergenic 30.0%
ACATGATAATCATAGCTGTT+AGG + chr2.3:67971232-67971251 None:intergenic 30.0%
AGCATTATGACGTATATTGT+AGG + chr2.3:67971203-67971222 None:intergenic 30.0%
ATATTTCCTTATGGGTTTCT+GGG - chr2.3:67970957-67970976 MS.gene05803:intron 30.0%
CATGATAATCATAGCTGTTA+GGG + chr2.3:67971231-67971250 None:intergenic 30.0%
CATGTAAATCTCAGTAAAAG+AGG - chr2.3:67971247-67971266 MS.gene05803:intron 30.0%
CATTATATTTGTAGATTCGC+CGG - chr2.3:67969534-67969553 MS.gene05803:CDS 30.0%
TCACAAATTCACTTGTTTGT+AGG - chr2.3:67969992-67970011 MS.gene05803:intron 30.0%
TCGCTGCAAAAAAATTGTAA+TGG - chr2.3:67970525-67970544 MS.gene05803:CDS 30.0%
TGATACTTTAAATGCTAACC+TGG + chr2.3:67970051-67970070 None:intergenic 30.0%
TGCAATCAAGTTCTTATGTA+AGG + chr2.3:67971142-67971161 None:intergenic 30.0%
TTCTTAATTAGAGATCTCCT+CGG + chr2.3:67970768-67970787 None:intergenic 30.0%
! AGAGTTGTCACATATTCTTT+TGG - chr2.3:67969066-67969085 MS.gene05803:intron 30.0%
! AGTTCATCAGTGTTTTCTTA+TGG + chr2.3:67971311-67971330 None:intergenic 30.0%
! CAAAATTATACAAGGTACCA+TGG - chr2.3:67969893-67969912 MS.gene05803:intron 30.0%
! GAATAACTCTAAAAGTGGAA+AGG + chr2.3:67970937-67970956 None:intergenic 30.0%
! GCAATTTGAGATTTGTATTG+AGG + chr2.3:67970806-67970825 None:intergenic 30.0%
! TTTGGAGCATAAAACATTTC+TGG + chr2.3:67970571-67970590 None:intergenic 30.0%
!! ACTATTCCATTGTTGATGAT+TGG - chr2.3:67971438-67971457 MS.gene05803:intron 30.0%
!! GAATGCAAAAGTTCTAAATC+AGG + chr2.3:67969435-67969454 None:intergenic 30.0%
!!! ATAAGATTTTTTCCCTTGGT+AGG - chr2.3:67969504-67969523 MS.gene05803:CDS 30.0%
AAATCTTCACCAACCATAAG+GGG + chr2.3:67969152-67969171 None:intergenic 35.0%
AAGTATCACACTCTCATGTT+AGG - chr2.3:67970063-67970082 MS.gene05803:CDS 35.0%
AGAAATCTTCACCAACCATA+AGG + chr2.3:67969154-67969173 None:intergenic 35.0%
AGGTCCACTTGAGTTTAAAA+AGG - chr2.3:67969324-67969343 MS.gene05803:CDS 35.0%
ATAATGTTACTCACCTACCA+AGG + chr2.3:67969520-67969539 None:intergenic 35.0%
ATACAAATGACCAAGTTGCA+GGG - chr2.3:67971471-67971490 MS.gene05803:CDS 35.0%
ATATCGAATTGACACATGGA+TGG + chr2.3:67968784-67968803 None:intergenic 35.0%
ATTATGGCCCTCATTGTTTA+AGG + chr2.3:67971652-67971671 None:intergenic 35.0%
CAATGGTGTAACAACTAGAA+AGG - chr2.3:67970355-67970374 MS.gene05803:intron 35.0%
CATATCTGATGAACTATACG+AGG - chr2.3:67970418-67970437 MS.gene05803:intron 35.0%
CATATTTCCTTATGGGTTTC+TGG - chr2.3:67970956-67970975 MS.gene05803:intron 35.0%
CTAACCTGGAGATTTATCAA+TGG + chr2.3:67970037-67970056 None:intergenic 35.0%
GAAATCTTCACCAACCATAA+GGG + chr2.3:67969153-67969172 None:intergenic 35.0%
GAGATTTGTATTGAGGAACT+TGG + chr2.3:67970799-67970818 None:intergenic 35.0%
GATTGATCAAAATTTCAGGG+TGG - chr2.3:67971713-67971732 MS.gene05803:CDS 35.0%
GCTTCAAGATCAAATTAGGT+AGG + chr2.3:67969366-67969385 None:intergenic 35.0%
TAATGTTACTCACCTACCAA+GGG + chr2.3:67969519-67969538 None:intergenic 35.0%
TCCACTTGAGTTTAAAAAGG+AGG - chr2.3:67969327-67969346 MS.gene05803:CDS 35.0%
TCTATAGTGAAGTTTCTTCC+TGG - chr2.3:67968388-67968407 MS.gene05803:intron 35.0%
TGAATCAGAGAAGAATCCAA+AGG - chr2.3:67968575-67968594 MS.gene05803:intron 35.0%
TGACATGACAATTCCATTCT+TGG - chr2.3:67971388-67971407 MS.gene05803:intron 35.0%
TGCAATACATAATTGAGCCA+TGG + chr2.3:67969913-67969932 None:intergenic 35.0%
TGTGCAAGTATAGATACACA+AGG - chr2.3:67971587-67971606 MS.gene05803:intron 35.0%
TGTGGCTTCAAGATCAAATT+AGG + chr2.3:67969370-67969389 None:intergenic 35.0%
TTTCTTCAGGGATACTTGTT+GGG - chr2.3:67970332-67970351 MS.gene05803:intron 35.0%
! ATTTTGAATACCCTGCAACT+TGG + chr2.3:67971484-67971503 None:intergenic 35.0%
! GTTTAGTTCAACTTATGGGA+AGG - chr2.3:67968306-67968325 MS.gene05803:CDS 35.0%
! TATTTTTCACAGCTCACATC+AGG - chr2.3:67970681-67970700 MS.gene05803:intron 35.0%
! TCCTCCTTTTTAAACTCAAG+TGG + chr2.3:67969331-67969350 None:intergenic 35.0%
! TCGATATGCACCGATATATT+TGG - chr2.3:67968797-67968816 MS.gene05803:intron 35.0%
! TTTTCTTCAGGGATACTTGT+TGG - chr2.3:67970331-67970350 MS.gene05803:intron 35.0%
!! GAAGGTACCTTAAACAATGA+GGG - chr2.3:67971642-67971661 MS.gene05803:intron 35.0%
!! TCTGGTCTTTTGCTTGAAAT+TGG - chr2.3:67968648-67968667 MS.gene05803:CDS 35.0%
!! TGAAGGTACCTTAAACAATG+AGG - chr2.3:67971641-67971660 MS.gene05803:intron 35.0%
AACTTGATTGCATGTGTTGC+AGG - chr2.3:67971150-67971169 MS.gene05803:intron 40.0%
AAGCGTTACTCGACAACTTA+AGG + chr2.3:67970832-67970851 None:intergenic 40.0%
AAGTTTCTTCCTGGATTCGA+AGG - chr2.3:67968397-67968416 MS.gene05803:intron 40.0%
AATCCCATTTGCTCATGGAA+TGG - chr2.3:67970391-67970410 MS.gene05803:intron 40.0%
ACATTCATGGACAAAGGTTC+GGG - chr2.3:67969387-67969406 MS.gene05803:CDS 40.0%
ACGATACTGAAAATTCTCCG+AGG - chr2.3:67970748-67970767 MS.gene05803:CDS 40.0%
ACTTGATTGCATGTGTTGCA+GGG - chr2.3:67971151-67971170 MS.gene05803:intron 40.0%
AGCGTTACTCGACAACTTAA+GGG + chr2.3:67970831-67970850 None:intergenic 40.0%
AGGATGATCCTCTCATACTA+TGG - chr2.3:67968595-67968614 MS.gene05803:intron 40.0%
AGTATGAGAGGATCATCCTT+TGG + chr2.3:67968594-67968613 None:intergenic 40.0%
ATCCCATTTGCTCATGGAAT+GGG - chr2.3:67970392-67970411 MS.gene05803:intron 40.0%
ATTGCTGGTGATTCATACTC+TGG - chr2.3:67969846-67969865 MS.gene05803:intron 40.0%
CACATTCATGGACAAAGGTT+CGG - chr2.3:67969386-67969405 MS.gene05803:CDS 40.0%
CATACAAATGACCAAGTTGC+AGG - chr2.3:67971470-67971489 MS.gene05803:CDS 40.0%
CATTCTTTAGGCTTGAACTC+TGG + chr2.3:67971752-67971771 None:intergenic 40.0%
CGAACCATTGATAAATCTCC+AGG - chr2.3:67970030-67970049 MS.gene05803:intron 40.0%
CTGTCTCCAATCATCAACAA+TGG + chr2.3:67971447-67971466 None:intergenic 40.0%
GATGACATTTGCAACTGTGA+AGG - chr2.3:67971624-67971643 MS.gene05803:intron 40.0%
GGTTTCAAGCACAAAAGGAA+GGG + chr2.3:67968425-67968444 None:intergenic 40.0%
GTGCATATCGAATTGACACA+TGG + chr2.3:67968788-67968807 None:intergenic 40.0%
GTTTCAAGCACAAAAGGAAG+GGG + chr2.3:67968424-67968443 None:intergenic 40.0%
GTTTCGACATGTTTAGTAGG+TGG - chr2.3:67971771-67971790 MS.gene05803:CDS 40.0%
TATGCAATCCCATTTGCTCA+TGG - chr2.3:67970386-67970405 MS.gene05803:intron 40.0%
TCTTATGCCACTACAGAATC+TGG - chr2.3:67969571-67969590 MS.gene05803:intron 40.0%
TGATCAAAATTTCAGGGTGG+AGG - chr2.3:67971716-67971735 MS.gene05803:CDS 40.0%
TGATCTTGAAGCCACATTCA+TGG - chr2.3:67969374-67969393 MS.gene05803:CDS 40.0%
TGTAAAGCCATGAACTAACC+CGG + chr2.3:67968446-67968465 None:intergenic 40.0%
TTGTTAAGGTATGTGGGAGT+TGG - chr2.3:67968516-67968535 MS.gene05803:intron 40.0%
! ATACTCTGGTATTGCTGTTC+CGG - chr2.3:67969860-67969879 MS.gene05803:intron 40.0%
! CAAAAAATCGGTGCTTGTGT+CGG + chr2.3:67970186-67970205 None:intergenic 40.0%
! CAAAGCACTACACATACGTA+AGG - chr2.3:67970999-67971018 MS.gene05803:intron 40.0%
! CTTCCTTTTGTGCTTGAAAC+CGG - chr2.3:67968424-67968443 MS.gene05803:intron 40.0%
! TTCCTTTTGTGCTTGAAACC+GGG - chr2.3:67968425-67968444 MS.gene05803:intron 40.0%
!! TGCAGGGAAGTATTGGAAAA+TGG - chr2.3:67971167-67971186 MS.gene05803:intron 40.0%
!! TGCTTTCAAGTTGCAACATG+TGG - chr2.3:67968364-67968383 MS.gene05803:intron 40.0%
!! TTGTTGATGATTGGAGACAG+TGG - chr2.3:67971447-67971466 MS.gene05803:CDS 40.0%
AACCCGGTTTCAAGCACAAA+AGG + chr2.3:67968430-67968449 None:intergenic 45.0%
AGTCCCATTCCATGAGCAAA+TGG + chr2.3:67970398-67970417 None:intergenic 45.0%
CATGCTTGTGTTGCCAAGAA+TGG + chr2.3:67971404-67971423 None:intergenic 45.0%
CATTAGCCCAGAAACCCATA+AGG + chr2.3:67970966-67970985 None:intergenic 45.0%
CATTCTTGGCAACACAAGCA+TGG - chr2.3:67971402-67971421 MS.gene05803:intron 45.0%
CCAACCATAAGGGGTTAACA+AGG + chr2.3:67969143-67969162 None:intergenic 45.0%
CCTTGTTAACCCCTTATGGT+TGG - chr2.3:67969140-67969159 MS.gene05803:CDS 45.0%
CGAACCTTTGTCCATGAATG+TGG + chr2.3:67969388-67969407 None:intergenic 45.0%
CGGTTTCAAGCACAAAAGGA+AGG + chr2.3:67968426-67968445 None:intergenic 45.0%
CTTAGCACCAGATTCTGTAG+TGG + chr2.3:67969581-67969600 None:intergenic 45.0%
GAAGCCACATTCATGGACAA+AGG - chr2.3:67969381-67969400 MS.gene05803:CDS 45.0%
GTCCCATTCCATGAGCAAAT+GGG + chr2.3:67970397-67970416 None:intergenic 45.0%
GTTACTCGACAACTTAAGGG+TGG + chr2.3:67970828-67970847 None:intergenic 45.0%
TAAGAGAAGCCTGTGGAAAC+CGG + chr2.3:67969556-67969575 None:intergenic 45.0%
! CATGTGTTGCAGGGAAGTAT+TGG - chr2.3:67971160-67971179 MS.gene05803:intron 45.0%
! TTTTAGACCCGCTATGTGAG+TGG - chr2.3:67970721-67970740 MS.gene05803:CDS 45.0%
!! AACAAGGCTAGCCTACATGA+GGG + chr2.3:67969127-67969146 None:intergenic 45.0%
!! CTAGCCTTGTTAACCCCTTA+TGG - chr2.3:67969136-67969155 MS.gene05803:CDS 45.0%
!! CTTGAAACCGGGTTAGTTCA+TGG - chr2.3:67968436-67968455 MS.gene05803:intron 45.0%
!! TAACAAGGCTAGCCTACATG+AGG + chr2.3:67969128-67969147 None:intergenic 45.0%
AGTGGCATAAGAGAAGCCTG+TGG + chr2.3:67969563-67969582 None:intergenic 50.0%
CCAGTGAGCCATAGTATGAG+AGG + chr2.3:67968606-67968625 None:intergenic 50.0%
CCTCTCATACTATGGCTCAC+TGG - chr2.3:67968603-67968622 MS.gene05803:intron 50.0%
CTCAACTTCCTCCCTCATGT+AGG - chr2.3:67969113-67969132 MS.gene05803:CDS 50.0%
CTCATACTATGGCTCACTGG+TGG - chr2.3:67968606-67968625 MS.gene05803:intron 50.0%
! CAGGGATACTTGTTGGGCAA+TGG - chr2.3:67970338-67970357 MS.gene05803:intron 50.0%
! TGCTTGTGTCGGTGCTTTCT+AGG + chr2.3:67970175-67970194 None:intergenic 50.0%
!! TGTGGGAGTTGGTGAGAAAG+AGG - chr2.3:67968527-67968546 MS.gene05803:intron 50.0%
CCAGACAAAGCAGAGCAACC+AGG + chr2.3:67968633-67968652 None:intergenic 55.0%
CCTGGTTGCTCTGCTTTGTC+TGG - chr2.3:67968630-67968649 MS.gene05803:CDS 55.0%
TCGTCACGCCACTCACATAG+CGG + chr2.3:67970732-67970751 None:intergenic 55.0%
TGTGCGATGTCGCGTGAATG+TGG + chr2.3:67971115-67971134 None:intergenic 55.0%
!! AAGGCTAGCCTACATGAGGG+AGG + chr2.3:67969124-67969143 None:intergenic 55.0%
!! GTAGATTCGCCGGTTTCCAC+AGG - chr2.3:67969544-67969563 MS.gene05803:CDS 55.0%
CGTCACGCCACTCACATAGC+GGG + chr2.3:67970731-67970750 None:intergenic 60.0%
GGAAGGGGTCCTTCGAATCC+AGG + chr2.3:67968409-67968428 None:intergenic 60.0%
! CTATGGCTCACTGGTGGACC+TGG - chr2.3:67968612-67968631 MS.gene05803:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 67968298 67971814 67968298 ID=MS.gene05803
chr2.3 mRNA 67968298 67971814 67968298 ID=MS.gene05803.t1;Parent=MS.gene05803
chr2.3 exon 67971666 67971814 67971666 ID=MS.gene05803.t1.exon1;Parent=MS.gene05803.t1
chr2.3 CDS 67971666 67971814 67971666 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
chr2.3 exon 67971443 67971588 67971443 ID=MS.gene05803.t1.exon2;Parent=MS.gene05803.t1
chr2.3 CDS 67971443 67971588 67971443 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
chr2.3 exon 67970710 67970786 67970710 ID=MS.gene05803.t1.exon3;Parent=MS.gene05803.t1
chr2.3 CDS 67970710 67970786 67970710 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
chr2.3 exon 67970464 67970586 67970464 ID=MS.gene05803.t1.exon4;Parent=MS.gene05803.t1
chr2.3 CDS 67970464 67970586 67970464 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
chr2.3 exon 67970206 67970308 67970206 ID=MS.gene05803.t1.exon5;Parent=MS.gene05803.t1
chr2.3 CDS 67970206 67970308 67970206 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
chr2.3 exon 67970061 67970098 67970061 ID=MS.gene05803.t1.exon6;Parent=MS.gene05803.t1
chr2.3 CDS 67970061 67970098 67970061 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
chr2.3 exon 67969673 67969771 67969673 ID=MS.gene05803.t1.exon7;Parent=MS.gene05803.t1
chr2.3 CDS 67969673 67969771 67969673 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
chr2.3 exon 67969501 67969587 67969501 ID=MS.gene05803.t1.exon8;Parent=MS.gene05803.t1
chr2.3 CDS 67969501 67969587 67969501 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
chr2.3 exon 67969270 67969419 67969270 ID=MS.gene05803.t1.exon9;Parent=MS.gene05803.t1
chr2.3 CDS 67969270 67969419 67969270 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
chr2.3 exon 67969092 67969163 67969092 ID=MS.gene05803.t1.exon10;Parent=MS.gene05803.t1
chr2.3 CDS 67969092 67969163 67969092 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
chr2.3 exon 67968822 67968940 67968822 ID=MS.gene05803.t1.exon11;Parent=MS.gene05803.t1
chr2.3 CDS 67968822 67968940 67968822 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
chr2.3 exon 67968620 67968733 67968620 ID=MS.gene05803.t1.exon12;Parent=MS.gene05803.t1
chr2.3 CDS 67968620 67968733 67968620 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
chr2.3 exon 67968467 67968512 67968467 ID=MS.gene05803.t1.exon13;Parent=MS.gene05803.t1
chr2.3 CDS 67968467 67968512 67968467 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
chr2.3 exon 67968298 67968381 67968298 ID=MS.gene05803.t1.exon14;Parent=MS.gene05803.t1
chr2.3 CDS 67968298 67968381 67968298 ID=cds.MS.gene05803.t1;Parent=MS.gene05803.t1
Gene Sequence

>MS.gene05803

ATGACAAAGTTTAGTTCAACTTATGGGAAGGTTCTTCTTTCTGTTTTTCTTTTATCATCACATATTTGCTTTCAAGTTGCAACATGTGGCTCTATAGTGAAGTTTCTTCCTGGATTCGAAGGACCCCTTCCTTTTGTGCTTGAAACCGGGTTAGTTCATGGCTTTACAAGTTTTTTTTAATCTATTATGGTATTTAAATATTTTCATATTCATATTTGTTGTTAAGGTATGTGGGAGTTGGTGAGAAAGAGGATGTGCAAGTGTTTTACTATTTCATTGAATCAGAGAAGAATCCAAAGGATGATCCTCTCATACTATGGCTCACTGGTGGACCTGGTTGCTCTGCTTTGTCTGGTCTTTTGCTTGAAATTGGTAATTGCAACTTGCTTTTCAAATTGTTCACTTTTGGATAGTCTTACAATAACCAATTGAAATTTTTGTTAATAAAGAAAAATGAATGGGTAAATGTTAGTGAGAATTTTTCCATCCATGTGTCAATTCGATATGCACCGATATATTTGGCAAAAGTTAATTTTTGAAAAAAAAAATTTATTTTCTATCATATAAAAAGTTATATTAATTAAATTATATTTATCAAAGTGTATGCGAAATAAATTTAATTTTTTTCAATTTGTTTTCTCGTAATAGTTAATATTGAATTATTTTACGATAAAATGTCAAAAAAATAGTATAATAATTGGAAATAATGAAATGATTTTTTTCTAATGAAATATGTGACAACTCTATGATTTTTTACTAAAAATTTATAGAGTTGTCACATATTCTTTTGGTTTATATTTAATATTTCGCATTCTCTCAACTTCCTCCCTCATGTAGGCTAGCCTTGTTAACCCCTTATGGTTGGTGAAGATTTCTAGATATAATTCATCTCTGTTGGAAAGACATTCAAATTAAAATTAGGAAGATCAATAGACACTGCAATGATACTAATTGAAGTGTCAATGTAAAGAAATTTACTGATGTGGTAAAATGATACTCATTGAATAATTGAACTGTAAATGTTACAGGTCCACTTGAGTTTAAAAAGGAGGAATACAATGGGAGCCTACCTAATTTGATCTTGAAGCCACATTCATGGACAAAGGTTCGGGTCATAGTCCACATTTTTTGTCACCTGATTTAGAACTTTTGCATTCAATGTATTTATCAAATTCTTTTAAATTATATATTTATTTACTAGTATTTATAAGATTTTTTCCCTTGGTAGGTGAGTAACATTATATTTGTAGATTCGCCGGTTTCCACAGGCTTCTCTTATGCCACTACAGAATCTGGTGCTAAGCGAAGCGACTCGATTCTAGTTCACCAAACATATGATTTTTTAAGGAAGGTATACATTTCACAATAATCACTTTTTTTATGTTTTAAAACTTTGTTTTTTTTTATTTTATTTTAAAAAGAAGTGTGTTTTTTTTAATTTTGTTAGTACTTATGTCAATCGGTCAACCAAATAACGAAAATTATGCTATAAATAAATCTTTGTAGTGGTTGGTTGATCATCCAAAGTTTCAATCAAATGAAATTTACATTGCTGGTGATTCATACTCTGGTATTGCTGTTCCGGTAATTGTTCACAAAATTATACAAGGTACCATGGCTCAATTATGTATTGCAGAACGAATGTGTTACAATTGATTTACTAGTATGTTGTAATTTTAATAAAGTTTAATTATTCACAAATTCACTTGTTTGTAGGAAATGAAAAAGGGATCGAACCATTGATAAATCTCCAGGTTAGCATTTAAAGTATCACACTCTCATGTTAGGAATGCATTTTTTCAACTAGTTCTGTAAATTACAAATGTATATTGTATGTTTCAAGAATGTGAAATTTGCATTTCAACTATTGAAAACCTAGAAAGCACCGACACAAGCACCGATTTTTTGATCAACTTAATGTTTCGATCAAATAGATTATGCATGACGTGTAATAAAACAATCGACTACATACAATAGCTTCAAGATTGATTATTTCGTTATATAACAGCTTTCAGATCAATTATGAAATGTTATTTTCTTCAGGGATACTTGTTGGGCAATGGTGTAACAACTAGAAAGGAAAAAAACTATGCAATCCCATTTGCTCATGGAATGGGACTCATATCTGATGAACTATACGAGGTAAATGAATTATACTTTCACAGTATCTAATTGAGTTATAAATCCTCCATACATAGCTAACTAATTGATATCTGCGATTTTCTAGTCGCTGCAAAAAAATTGTAATGGAGACTATGTAAATGCAGAGACCAGAAATGTTTTATGCTCCAAAGATATCAATTCATTCAATGAGGTATATGTTAATTTTATTTTTGTCAAATTTCGCGAGAATTACTAGTCACAAAAATGAACATCATTCTTCTATTTTTCACAGCTCACATCAGGACTTAATACAGCACATATTTTAGACCCGCTATGTGAGTGGCGTGACGATACTGAAAATTCTCCGAGGAGATCTCTAATTAAGAATCATTTCTCCAAGTTCCTCAATACAAATCTCAAATTGCCACCCTTAAGTTGTCGAGTAACGCTTCTTACTATATATATACCATTTGCTGCATTATAATTATAAACATATATAAAGCTCAATTTTTTTGTTAACTTGATGTTTGATTCCTTTCCACTTTTAGAGTTATTCATATTTCCTTATGGGTTTCTGGGCTAATGATGATAATGTTCGCAAAGCACTACACATACGTAAGGTATATTATTGATACATATATGCGAAGTTGTAATTTCTTCATGCTATCTGTATATTCATATTTCACACCTTTGTTTTCCATAAATTTTTATCCACATTCACGCGACATCGCACATAGTCCTTACATAAGAACTTGATTGCATGTGTTGCAGGGAAGTATTGGAAAATGGCATCGTTGCACCTACAATATACGTCATAATGCTGATATCCCTAACAGCTATGATTATCATGTAAATCTCAGTAAAAGAGGAATTCGTTCACTAATTTACAAGTCAGTAATCTTTCTCTCCATAAGAAAACACTGATGAACTAGTCTTATAATTTCTTATGGAAAATTGGTTTTATATTTACAATAACAGTGGCGATCATGACATGACAATTCCATTCTTGGCAACACAAGCATGGATAAGATCTTTGAACTATTCCATTGTTGATGATTGGAGACAGTGGCATACAAATGACCAAGTTGCAGGGTATTCAAAATTTCTAATTATTATAGTCATTATATATAATAACCATGATAAATTTATGACCAAGTTGAATTAATATTTCTCTTTGTTTTAACTGTGTGCAAGTATAGATACACAAGGACTTACTCGAATCAGATGACATTTGCAACTGTGAAGGTACCTTAAACAATGAGGGCCATAATCATGTGTTAAAAATAATGACTAGTTTATTTACTCTATGAATGATTGATCAAAATTTCAGGGTGGAGGACACACAGCTCCAGAGTTCAAGCCTAAAGAATGTTTCGACATGTTTAGTAGGTGGTTATCTAAAAAAGCTTTGTAA
Protein sequence

>MS.gene05803.t1

MTKFSSTYGKVLLSVFLLSSHICFQVATCGSIVKFLPGFEGPLPFVLETGYVGVGEKEDVQVFYYFIESEKNPKDDPLILWLTGGPGCSALSGLLLEIGPLEFKKEEYNGSLPNLILKPHSWTKVSNIIFVDSPVSTGFSYATTESGAKRSDSILVHQTYDFLRKWLVDHPKFQSNEIYIAGDSYSGIAVPVIVHKIIQGNEKGIEPLINLQGYLLGNGVTTRKEKNYAIPFAHGMGLISDELYESLQKNCNGDYVNAETRNVLCSKDINSFNELTSGLNTAHILDPLCEWRDDTENSPRRSLIKNHFSKFLNTNLKLPPLSCRSYSYFLMGFWANDDNVRKALHIRKGSIGKWHRCTYNIRHNADIPNSYDYHVNLSKRGIRSLIYNGDHDMTIPFLATQAWIRSLNYSIVDDWRQWHTNDQVAGYTRTYSNQMTFATVKGGGHTAPEFKPKECFDMFSRWLSKKAL