AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43593

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43593.t1 MTR_2g018590 93.603 469 29 1 1 468 1 469 0.0 923
MS.gene43593.t1 MTR_2g018600 85.928 469 65 1 1 468 1 469 0.0 836
MS.gene43593.t1 MTR_2g018630 74.667 450 105 3 20 468 24 465 0.0 707
MS.gene43593.t1 MTR_3g100420 64.668 467 155 4 12 468 30 496 0.0 629
MS.gene43593.t1 MTR_4g021520 62.584 449 161 5 21 468 28 470 0.0 583
MS.gene43593.t1 MTR_4g021510 61.915 449 164 5 21 468 27 469 0.0 572
MS.gene43593.t1 MTR_4g021500 60.948 443 166 5 21 462 28 464 0.0 563
MS.gene43593.t1 MTR_4g021500 59.028 432 150 6 39 468 481 887 1.68e-176 518
MS.gene43593.t1 MTR_4g021500 60.948 443 166 5 21 462 28 464 0.0 563
MS.gene43593.t1 MTR_4g021500 60.417 432 162 5 39 468 481 905 0.0 545
MS.gene43593.t1 MTR_4g021490 59.465 449 175 5 21 468 28 470 0.0 558
MS.gene43593.t1 MTR_4g021535 57.609 460 188 5 10 468 13 466 0.0 555
MS.gene43593.t1 MTR_2g018630 71.662 367 95 3 103 468 2 360 0.0 550
MS.gene43593.t1 MTR_4g021530 57.391 460 189 6 10 468 17 470 0.0 543
MS.gene43593.t1 MTR_4g021520 61.671 407 149 5 21 426 28 428 0.0 513
MS.gene43593.t1 MTR_4g021535 50.435 460 174 6 10 468 13 419 1.02e-160 461
MS.gene43593.t1 MTR_3g045770 43.573 459 237 5 11 464 9 450 4.51e-136 400
MS.gene43593.t1 MTR_2g009200 41.775 462 227 8 16 468 11 439 1.18e-125 372
MS.gene43593.t1 MTR_8g011350 39.321 501 265 8 1 468 1 495 1.34e-124 372
MS.gene43593.t1 MTR_7g051430 39.919 491 257 8 11 468 10 495 6.53e-123 367
MS.gene43593.t1 MTR_7g080180 40.121 496 254 10 11 468 8 498 9.52e-122 365
MS.gene43593.t1 MTR_8g011350 39.030 474 250 8 1 441 1 468 8.19e-112 338
MS.gene43593.t1 MTR_7g080180 39.207 454 233 10 11 426 8 456 3.28e-102 313
MS.gene43593.t1 MTR_7g051430 38.049 410 216 8 11 387 10 414 1.29e-85 269
MS.gene43593.t1 MTR_4g075150 32.051 468 292 14 13 468 9 462 9.76e-68 224
MS.gene43593.t1 MTR_4g075150 32.265 468 289 14 13 468 9 460 2.19e-67 223
MS.gene43593.t1 MTR_4g122960 29.828 466 298 16 13 464 20 470 5.19e-58 199
MS.gene43593.t1 MTR_2g012860 30.538 465 290 15 18 468 27 472 5.50e-58 199
MS.gene43593.t1 MTR_8g099930 31.602 462 274 16 24 468 25 461 1.08e-57 197
MS.gene43593.t1 MTR_7g013790 29.688 448 277 13 36 468 39 463 4.49e-57 196
MS.gene43593.t1 MTR_4g122950 29.614 466 301 16 13 464 20 472 5.44e-57 196
MS.gene43593.t1 MTR_2g063000 28.750 480 298 16 5 464 3 458 2.47e-56 194
MS.gene43593.t1 MTR_2g062980 29.325 474 302 14 2 463 4 456 4.96e-56 193
MS.gene43593.t1 MTR_1g095540 29.936 471 292 15 12 466 5 453 5.37e-56 193
MS.gene43593.t1 MTR_2g012850 29.663 445 280 14 32 463 35 459 6.48e-56 193
MS.gene43593.t1 MTR_2g062990 28.903 474 304 15 2 463 4 456 9.18e-56 192
MS.gene43593.t1 MTR_5g072570 28.842 475 300 16 12 468 6 460 1.30e-55 192
MS.gene43593.t1 MTR_3g434850 29.478 441 275 14 40 468 37 453 1.67e-55 191
MS.gene43593.t1 MTR_3g498755 28.452 478 274 13 22 468 4 444 3.92e-55 190
MS.gene43593.t1 MTR_1g021010 32.648 438 255 15 33 463 76 480 8.10e-55 191
MS.gene43593.t1 MTR_3g079590 30.184 434 267 12 36 463 81 484 1.24e-54 190
MS.gene43593.t1 MTR_1g095560 29.916 478 293 17 9 468 2 455 1.45e-54 189
MS.gene43593.t1 MTR_2g081620 30.337 445 264 16 36 464 44 458 1.02e-53 187
MS.gene43593.t1 MTR_3g034290 28.798 441 283 12 33 468 75 489 3.47e-53 186
MS.gene43593.t1 MTR_0003s0560 29.213 445 274 13 33 468 74 486 7.88e-53 185
MS.gene43593.t1 MTR_4g089155 29.478 441 288 14 32 463 53 479 1.21e-52 184
MS.gene43593.t1 MTR_7g021130 28.512 477 293 14 11 468 9 456 3.61e-52 182
MS.gene43593.t1 MTR_3g079570 29.398 432 272 12 36 463 82 484 4.46e-52 183
MS.gene43593.t1 MTR_2g449850 28.935 432 263 14 51 466 56 459 9.56e-52 182
MS.gene43593.t1 MTR_3g079560 29.452 438 271 13 33 463 78 484 1.04e-51 182
MS.gene43593.t1 MTR_3g090130 29.333 450 283 14 32 468 28 455 3.08e-51 180
MS.gene43593.t1 MTR_1g020090 32.273 440 251 14 33 463 90 491 3.94e-51 181
MS.gene43593.t1 MTR_3g090150 28.132 455 283 15 32 468 34 462 1.06e-49 176
MS.gene43593.t1 MTR_3g079600 28.833 437 278 13 33 463 76 485 2.47e-49 176
MS.gene43593.t1 MTR_1g095560 28.039 510 293 18 9 468 2 487 3.12e-48 173
MS.gene43593.t1 MTR_1g021020 29.773 440 264 15 33 463 78 481 9.73e-48 171
MS.gene43593.t1 MTR_3g079620 28.959 442 270 14 36 463 81 492 1.11e-47 171
MS.gene43593.t1 MTR_2g063000 27.673 477 276 16 5 464 3 427 2.41e-47 169
MS.gene43593.t1 MTR_3g079630 28.539 445 268 15 36 463 81 492 1.48e-46 169
MS.gene43593.t1 MTR_2g095750 27.009 448 276 10 36 466 85 498 2.76e-45 165
MS.gene43593.t1 MTR_4g057585 27.650 434 254 11 49 463 98 490 5.91e-45 164
MS.gene43593.t1 MTR_4g057595 27.650 434 254 11 49 463 99 491 6.48e-45 164
MS.gene43593.t1 MTR_4g057600 26.328 433 269 10 48 466 96 492 1.32e-43 160
MS.gene43593.t1 MTR_1g040370 26.351 444 246 11 40 468 37 414 1.74e-43 158
MS.gene43593.t1 MTR_1g050545 26.667 435 256 10 49 462 85 477 1.96e-43 160
MS.gene43593.t1 MTR_2g095730 28.571 469 260 19 30 466 70 495 1.24e-42 158
MS.gene43593.t1 MTR_4g057570 26.411 443 256 13 50 466 92 490 1.57e-41 154
MS.gene43593.t1 MTR_1g094155 27.230 426 264 14 49 462 92 483 8.50e-41 153
MS.gene43593.t1 MTR_1g102130 26.637 443 242 16 49 468 107 489 2.34e-38 146
MS.gene43593.t1 MTR_5g097180 24.490 441 268 14 48 468 42 437 1.46e-37 143
MS.gene43593.t1 MTR_3g034290 31.618 272 174 7 33 301 75 337 2.45e-36 139
MS.gene43593.t1 MTR_2g018650 54.762 126 37 3 316 432 8 122 3.18e-36 130
MS.gene43593.t1 MTR_0003s0560 31.010 287 183 9 33 316 74 348 5.73e-35 134
MS.gene43593.t1 MTR_4g084710 24.208 442 266 14 49 468 45 439 2.02e-30 123
MS.gene43593.t1 MTR_2g095710 25.169 445 277 13 36 466 79 481 2.77e-29 120
MS.gene43593.t1 MTR_0075s0010 36.957 92 44 3 62 146 35 119 5.83e-11 63.9
MS.gene43593.t1 MTR_2g084530 23.385 449 270 18 48 458 34 446 6.98e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43593.t1 AT1G33540 54.875 441 195 4 30 468 26 464 1.05e-175 501
MS.gene43593.t1 AT5G09640 52.688 465 207 8 12 468 6 465 1.89e-175 500
MS.gene43593.t1 AT2G22920 54.977 442 168 5 30 468 22 435 4.96e-175 498
MS.gene43593.t1 AT1G73300 55.730 445 168 4 25 468 25 441 7.45e-175 498
MS.gene43593.t1 AT1G73300 55.730 445 168 4 25 468 25 441 7.45e-175 498
MS.gene43593.t1 AT2G22970 54.545 440 171 4 30 468 22 433 1.66e-174 497
MS.gene43593.t1 AT2G22980 55.856 444 161 6 27 468 20 430 3.86e-174 496
MS.gene43593.t1 AT1G73280 52.665 469 190 5 4 468 1 441 1.22e-173 495
MS.gene43593.t1 AT5G36180 56.180 445 166 4 25 468 25 441 1.24e-173 495
MS.gene43593.t1 AT2G23000 54.773 440 174 4 30 468 22 437 2.08e-171 489
MS.gene43593.t1 AT2G23000 54.773 440 174 4 30 468 22 437 2.08e-171 489
MS.gene43593.t1 AT1G73310 55.056 445 171 4 25 468 25 441 6.11e-171 488
MS.gene43593.t1 AT3G10450 53.034 445 180 4 25 468 21 437 7.38e-171 488
MS.gene43593.t1 AT2G22990 55.000 440 171 5 30 468 20 433 2.54e-170 486
MS.gene43593.t1 AT1G73290 53.483 445 176 5 25 468 24 438 2.43e-169 484
MS.gene43593.t1 AT2G23010 52.070 459 195 4 11 468 3 437 2.85e-167 478
MS.gene43593.t1 AT1G73270 54.091 440 173 3 30 468 30 441 6.48e-167 478
MS.gene43593.t1 AT1G73270 54.091 440 173 3 30 468 30 441 7.32e-167 478
MS.gene43593.t1 AT2G23010 51.634 459 197 4 11 468 3 437 1.63e-165 474
MS.gene43593.t1 AT1G33540 54.524 420 187 4 51 468 4 421 4.35e-165 472
MS.gene43593.t1 AT2G22970 54.348 414 161 3 30 442 22 408 1.23e-163 468
MS.gene43593.t1 AT2G22970 54.327 416 162 3 30 444 22 410 1.27e-163 469
MS.gene43593.t1 AT3G12203 50.655 458 194 4 12 468 11 437 2.20e-163 469
MS.gene43593.t1 AT3G12203 51.591 440 183 3 30 468 6 416 2.80e-162 465
MS.gene43593.t1 AT2G22980 55.502 418 152 5 27 442 20 405 5.17e-162 464
MS.gene43593.t1 AT2G22980 54.273 433 162 6 27 455 20 420 1.08e-161 464
MS.gene43593.t1 AT2G22990 53.409 440 169 6 30 468 20 424 1.46e-161 463
MS.gene43593.t1 AT3G12240 51.121 446 189 4 24 468 19 436 1.07e-159 459
MS.gene43593.t1 AT3G12230 51.016 443 188 4 27 468 21 435 7.02e-158 454
MS.gene43593.t1 AT3G10450 52.029 419 172 4 51 468 4 394 7.43e-158 453
MS.gene43593.t1 AT3G10450 52.506 419 171 3 25 442 21 412 2.60e-157 452
MS.gene43593.t1 AT2G22990 53.023 430 173 5 30 455 20 424 1.02e-156 452
MS.gene43593.t1 AT2G22990 54.106 414 164 4 30 442 20 408 2.48e-156 450
MS.gene43593.t1 AT2G22920 53.616 401 156 4 30 427 22 395 1.77e-153 442
MS.gene43593.t1 AT1G73310 53.676 408 161 3 25 431 25 405 2.00e-151 438
MS.gene43593.t1 AT3G12220 49.661 443 194 3 27 468 21 435 2.30e-151 438
MS.gene43593.t1 AT1G73290 52.723 404 161 4 25 427 24 398 3.99e-148 428
MS.gene43593.t1 AT3G10450 51.399 393 163 3 51 442 4 369 1.78e-144 418
MS.gene43593.t1 AT3G12203 49.755 408 175 3 62 468 1 379 2.59e-142 413
MS.gene43593.t1 AT3G12220 48.449 419 187 3 51 468 15 405 7.36e-138 402
MS.gene43593.t1 AT4G12910 40.909 484 254 7 10 463 11 492 2.86e-128 381
MS.gene43593.t1 AT5G36180 52.589 367 145 4 103 468 1 339 2.51e-127 373
MS.gene43593.t1 AT5G36180 52.589 367 145 4 103 468 1 339 2.51e-127 373
MS.gene43593.t1 AT1G73280 50.000 388 163 4 4 387 1 361 9.81e-127 373
MS.gene43593.t1 AT1G73280 50.954 367 151 4 103 468 1 339 7.80e-126 369
MS.gene43593.t1 AT2G22980 52.055 365 139 6 27 387 20 352 3.18e-122 360
MS.gene43593.t1 AT3G25420 38.351 485 266 7 11 464 8 490 5.13e-122 365
MS.gene43593.t1 AT2G22990 52.174 345 138 5 125 468 1 319 6.01e-119 351
MS.gene43593.t1 AT2G22990 51.594 345 140 5 125 468 1 319 1.36e-117 347
MS.gene43593.t1 AT1G73280 51.170 342 138 4 128 468 1 314 2.54e-116 344
MS.gene43593.t1 AT5G36180 52.047 342 136 3 103 443 1 315 3.91e-116 344
MS.gene43593.t1 AT2G22970 51.097 319 129 2 30 348 22 313 1.18e-113 337
MS.gene43593.t1 AT2G22920 50.938 320 129 3 30 348 22 314 2.63e-112 334
MS.gene43593.t1 AT4G12910 39.821 447 237 7 10 426 11 455 2.79e-109 331
MS.gene43593.t1 AT5G36180 49.425 348 146 4 103 447 1 321 1.62e-105 317
MS.gene43593.t1 AT2G22980 50.938 320 123 4 125 442 1 288 9.21e-105 314
MS.gene43593.t1 AT1G73310 49.240 329 139 3 104 431 2 303 6.03e-104 313
MS.gene43593.t1 AT1G73290 57.088 261 109 2 30 289 29 287 2.90e-103 310
MS.gene43593.t1 AT2G22920 51.408 284 107 5 188 468 1 256 1.94e-94 286
MS.gene43593.t1 AT3G12220 56.170 235 102 1 27 260 21 255 6.96e-84 263
MS.gene43593.t1 AT3G12220 52.414 145 68 1 324 468 240 383 3.64e-47 167
MS.gene43593.t1 AT3G52010 32.366 448 264 12 32 468 64 483 2.82e-68 226
MS.gene43593.t1 AT3G52000 31.166 446 268 10 32 468 63 478 1.92e-67 224
MS.gene43593.t1 AT5G23210 31.489 470 285 16 23 468 38 494 9.29e-65 217
MS.gene43593.t1 AT2G05850 30.684 453 266 16 32 468 63 483 6.82e-64 214
MS.gene43593.t1 AT3G52020 32.373 451 259 13 32 468 79 497 1.29e-62 211
MS.gene43593.t1 AT4G15100 29.735 491 311 14 1 468 1 480 1.91e-62 211
MS.gene43593.t1 AT4G30610 32.527 455 262 17 33 468 33 461 1.83e-61 207
MS.gene43593.t1 AT3G63470 30.090 442 278 10 32 468 81 496 2.53e-60 205
MS.gene43593.t1 AT4G30810 27.922 462 307 12 15 468 18 461 1.30e-59 203
MS.gene43593.t1 AT2G22960 64.706 136 47 1 333 468 50 184 2.19e-59 193
MS.gene43593.t1 AT5G08260 29.425 452 289 14 32 468 39 475 8.16e-58 198
MS.gene43593.t1 AT1G61130 30.512 449 278 15 32 467 31 458 6.12e-57 196
MS.gene43593.t1 AT3G17180 28.474 439 276 13 49 468 55 474 6.98e-57 196
MS.gene43593.t1 AT1G11080 31.707 451 280 14 32 467 50 487 8.58e-57 196
MS.gene43593.t1 AT1G11080 31.707 451 280 14 32 467 50 487 1.04e-56 196
MS.gene43593.t1 AT2G35780 30.337 445 271 15 36 468 33 450 4.20e-56 193
MS.gene43593.t1 AT2G24010 29.955 444 281 12 32 468 30 450 1.39e-55 192
MS.gene43593.t1 AT2G24000 29.821 446 287 12 33 468 31 460 2.04e-54 189
MS.gene43593.t1 AT1G28110 29.556 450 279 13 33 468 32 457 7.01e-54 187
MS.gene43593.t1 AT1G28110 29.556 450 279 13 33 468 32 457 7.01e-54 187
MS.gene43593.t1 AT2G33530 30.222 450 272 16 36 468 37 461 1.26e-53 187
MS.gene43593.t1 AT1G43780 29.646 452 284 14 32 468 37 469 1.79e-53 187
MS.gene43593.t1 AT2G35770 29.933 451 264 12 36 468 41 457 1.96e-53 186
MS.gene43593.t1 AT5G23210 30.986 426 258 15 23 424 38 451 4.99e-53 185
MS.gene43593.t1 AT3G02110 29.888 445 285 14 36 468 41 470 4.74e-52 182
MS.gene43593.t1 AT3G07990 28.381 451 274 13 36 468 37 456 7.56e-52 182
MS.gene43593.t1 AT2G12480 28.508 449 286 13 32 468 31 456 1.24e-51 181
MS.gene43593.t1 AT2G24010 28.758 459 282 13 32 468 30 465 2.07e-50 178
MS.gene43593.t1 AT5G42240 29.075 454 283 15 32 468 32 463 1.04e-49 176
MS.gene43593.t1 AT5G22980 29.698 431 254 15 49 466 100 494 4.12e-49 176
MS.gene43593.t1 AT2G12480 27.455 448 268 12 32 468 31 432 4.62e-49 174
MS.gene43593.t1 AT5G23210 29.950 404 251 14 85 468 8 399 7.42e-49 172
MS.gene43593.t1 AT5G23210 29.777 403 251 14 86 468 8 398 4.64e-48 170
MS.gene43593.t1 AT2G12480 27.232 448 267 12 32 468 31 430 4.71e-48 171
MS.gene43593.t1 AT5G42230 28.667 450 290 15 32 468 28 459 7.31e-47 169
MS.gene43593.t1 AT5G42230 28.667 450 290 15 32 468 28 459 7.31e-47 169
MS.gene43593.t1 AT2G12480 27.171 449 267 13 32 468 31 431 1.98e-46 167
MS.gene43593.t1 AT3G17180 27.889 398 249 13 49 427 55 433 4.61e-46 166
MS.gene43593.t1 AT2G24010 27.273 484 282 14 32 468 1 461 2.11e-45 164
MS.gene43593.t1 AT3G45010 27.169 438 266 12 45 466 99 499 6.15e-45 164
MS.gene43593.t1 AT3G10410 27.594 424 266 11 49 463 101 492 2.08e-44 163
MS.gene43593.t1 AT5G23210 28.969 359 224 13 86 424 8 355 2.35e-36 138
MS.gene43593.t1 AT2G24010 29.462 353 207 11 32 364 1 331 8.41e-33 128
MS.gene43593.t1 AT1G15000 23.953 430 269 15 48 463 36 421 8.04e-31 124
MS.gene43593.t1 AT3G56540 38.217 157 90 3 32 187 79 229 4.59e-30 118
MS.gene43593.t1 AT5G22960 29.697 165 91 2 48 212 35 174 9.08e-25 101
MS.gene43593.t1 AT2G24000 25.172 290 200 7 186 468 118 397 4.74e-20 92.4
MS.gene43593.t1 AT2G27920 23.246 456 279 23 50 467 37 459 1.97e-13 72.4

Find 98 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATTTCCTAATGGGTTTC+TGG 0.042911 2.2:-66167042 MS.gene43593:CDS
TTTGGAACATAAAACATTTC+TGG 0.145142 2.2:+66167435 None:intergenic
ATTGAATGAATTGATATCTT+TGG 0.232350 2.2:+66167417 None:intergenic
TTGTTTGTAGGAAATGAAAA+AGG 0.248089 2.2:-66168040 MS.gene43593:intron
ACAAAGTTTAGTTCAACTTA+TGG 0.250544 2.2:-66169751 MS.gene43593:CDS
TGACATGACAATTCCATTCT+TGG 0.262363 2.2:-66166609 MS.gene43593:CDS
TCTGGTCTTATGCTTGAAAT+TGG 0.286687 2.2:-66169404 MS.gene43593:intron
ATTTCATTTGATTGAAACTT+TGG 0.310987 2.2:+66168235 None:intergenic
AGGTCCACTTGAGTTTAAAA+AGG 0.317116 2.2:-66168728 MS.gene43593:CDS
TGTGGCTTCAAGATCAAATT+AGG 0.326932 2.2:+66168685 None:intergenic
TCAAATGAAATTTACATTGC+TGG 0.330344 2.2:-66168223 MS.gene43593:CDS
ACATGATAATCATAGCTGTT+AGG 0.338091 2.2:+66166768 None:intergenic
TGATCTTGAAGCCACATTCA+TGG 0.345897 2.2:-66168678 MS.gene43593:CDS
ATAATTTCTTGAACAATTAC+CGG 0.348002 2.2:+66168175 None:intergenic
CAAAGTTTAGTTCAACTTAT+GGG 0.357058 2.2:-66169750 MS.gene43593:CDS
AGGATGATCCTCTCATACTA+TGG 0.360528 2.2:-66169457 MS.gene43593:CDS
AAGCGTTACTCGACAACTTA+AGG 0.360759 2.2:+66167171 None:intergenic
GAGTTATTCATATTTCCTAA+TGG 0.362015 2.2:-66167050 MS.gene43593:CDS
AGTTATTCATATTTCCTAAT+GGG 0.367070 2.2:-66167049 MS.gene43593:CDS
ACTATTCCATTGTTGATGAT+TGG 0.372894 2.2:-66166559 MS.gene43593:CDS
CCTGGTTGCTCTGCTTTGTC+TGG 0.374838 2.2:-66169422 MS.gene43593:CDS
GACACACAGCTCCAGAGTTC+CGG 0.390768 2.2:-66165768 MS.gene43593:CDS
ATATTTCCTAATGGGTTTCT+GGG 0.390815 2.2:-66167041 MS.gene43593:CDS
TCTATAGTGAAGTTTCTTCC+TGG 0.395633 2.2:-66169664 MS.gene43593:CDS
CCTCTCATACTATGGCTAAC+TGG 0.396512 2.2:-66169449 MS.gene43593:CDS
CATACAAATGACCAAGTTGC+AGG 0.404512 2.2:-66166527 MS.gene43593:CDS
ATACTCTGGTATTGCTGTTC+CGG 0.407889 2.2:-66168194 MS.gene43593:CDS
TTTCTTCAGGGATACTTGTT+GGG 0.407975 2.2:-66167712 MS.gene43593:intron
GTTATCTAAGAGGCCTTTGT+AGG 0.415530 2.2:-66165713 None:intergenic
TGCAGGGAAGTATTGGAAAA+TGG 0.419352 2.2:-66166830 MS.gene43593:intron
TCTTATGCCACTACAGAATC+TGG 0.426608 2.2:-66168481 MS.gene43593:CDS
CATGATAATCATAGCTGTTA+GGG 0.426927 2.2:+66166769 None:intergenic
AGTCCCATTCCATGAGCAAA+TGG 0.428128 2.2:+66167649 None:intergenic
GCACAAAAGGAAGGGGTCCT+TGG 0.428977 2.2:+66169638 None:intergenic
ATCCCATTTGCTCATGGAAT+GGG 0.431219 2.2:-66167652 MS.gene43593:CDS
ATATTTGTTGTTAAGGTATG+TGG 0.432299 2.2:-66169543 MS.gene43593:intron
GCAATTTGAGATTTGTATTG+AGG 0.443024 2.2:+66167197 None:intergenic
TTGTTAAGGTATGTGGGAGT+TGG 0.444634 2.2:-66169536 MS.gene43593:intron
CATGTAAATCTAAGTAGAAA+AGG 0.448093 2.2:-66166750 MS.gene43593:CDS
CATTAGCCCAGAAACCCATT+AGG 0.452810 2.2:+66167035 None:intergenic
AGTATGAGAGGATCATCCTT+TGG 0.453644 2.2:+66169461 None:intergenic
CAGGGATACTTGTTGGGCAA+TGG 0.453796 2.2:-66167706 MS.gene43593:CDS
CATGTGTTGCAGGGAAGTAT+TGG 0.455548 2.2:-66166837 MS.gene43593:intron
TAAGAGAAGCCTGTGGAAAC+CGG 0.462321 2.2:+66168499 None:intergenic
CATGCTTGTGTTGCCAAGAA+TGG 0.464724 2.2:+66166596 None:intergenic
GGAAGGGGTCCTTGGAATCC+AGG 0.464903 2.2:+66169646 None:intergenic
AACATGTCAAAACATTCTTT+AGG 0.471328 2.2:+66165745 None:intergenic
CATTATATTTGTAGATTCGC+CGG 0.473076 2.2:-66168518 MS.gene43593:CDS
CATTCTTTAGGCCGGAACTC+TGG 0.476789 2.2:+66165757 None:intergenic
GAGATTTGTATTGAGGAACT+TGG 0.479830 2.2:+66167204 None:intergenic
AGCATTATGACGTATATTGT+AGG 0.481619 2.2:+66166797 None:intergenic
AACCCGGTTTCAAGCACAAA+AGG 0.485577 2.2:+66169625 None:intergenic
CGAACCATTGATAAATCTCC+AGG 0.488461 2.2:-66168014 MS.gene43593:intron
ATTGCTGGTGATTCATACTC+TGG 0.492272 2.2:-66168208 MS.gene43593:CDS
TATGCAATCCCATTTGCTCA+TGG 0.502070 2.2:-66167658 MS.gene43593:CDS
TATTTGTTGTTAAGGTATGT+GGG 0.503693 2.2:-66169542 MS.gene43593:intron
TTAGTAGGTGGTTATCTAAG+AGG 0.504447 2.2:-66165723 MS.gene43593:CDS
AAGTTTCTTCCTGGATTCCA+AGG 0.510461 2.2:-66169655 MS.gene43593:CDS
GTAGATTCGCCGGTTTCCAC+AGG 0.511485 2.2:-66168508 MS.gene43593:CDS
TGCTTTCAAGTTGCAACATG+TGG 0.513785 2.2:-66169688 MS.gene43593:CDS
TGATCAAAATTTCAGGGTGG+AGG 0.517860 2.2:-66165790 MS.gene43593:intron
CTAACCTGGAGATTTATCAA+TGG 0.519088 2.2:+66168010 None:intergenic
AATCCCATTTGCTCATGGAA+TGG 0.520162 2.2:-66167653 MS.gene43593:CDS
CTATGGCTAACTGGTGGACC+TGG 0.525006 2.2:-66169440 MS.gene43593:CDS
GGTTTCAAGCACAAAAGGAA+GGG 0.526633 2.2:+66169630 None:intergenic
AGCGTTACTCGACAACTTAA+GGG 0.526961 2.2:+66167172 None:intergenic
CGGTTTCAAGCACAAAAGGA+AGG 0.531662 2.2:+66169629 None:intergenic
CGAACCTTTGTCCATGAATG+TGG 0.535010 2.2:+66168667 None:intergenic
CAATGGTGTAACAACTAGAA+AGG 0.545007 2.2:-66167689 MS.gene43593:CDS
TGTGGGAGTTGGTGAGAAAG+AGG 0.550305 2.2:-66169525 MS.gene43593:CDS
CATTCTTGGCAACACAAGCA+TGG 0.552262 2.2:-66166595 MS.gene43593:CDS
GTCCCATTCCATGAGCAAAT+GGG 0.553573 2.2:+66167650 None:intergenic
TGTCAAAACATTCTTTAGGC+CGG 0.557083 2.2:+66165749 None:intergenic
GTTTAGTTCAACTTATGGGA+AGG 0.563304 2.2:-66169746 MS.gene43593:CDS
CCAAGTGTTTATTCCTACAA+AGG 0.566030 2.2:+66165700 None:intergenic
CCAGTTAGCCATAGTATGAG+AGG 0.570952 2.2:+66169449 None:intergenic
GTTTCAAGCACAAAAGGAAG+GGG 0.572128 2.2:+66169631 None:intergenic
CCAGACAAAGCAGAGCAACC+AGG 0.587330 2.2:+66169422 None:intergenic
CAAAGCAATACACATACGTA+AGG 0.592494 2.2:-66166999 MS.gene43593:intron
ATTGTTCAAGAAATTATACA+AGG 0.595697 2.2:-66168169 MS.gene43593:intron
CGTCACGCCACTCACATAGC+GGG 0.606581 2.2:+66167272 None:intergenic
GATGACATTTGCAACTGTGA+AGG 0.610163 2.2:-66165882 MS.gene43593:intron
TTCTTAATTAGAGATCTCCT+CGG 0.615291 2.2:+66167235 None:intergenic
CTCATACTATGGCTAACTGG+TGG 0.626625 2.2:-66169446 MS.gene43593:CDS
GCTTCAAGATCAAATTAGGT+AGG 0.628776 2.2:+66168689 None:intergenic
CTGTCTCCAATCATCAACAA+TGG 0.629618 2.2:+66166553 None:intergenic
GAAGCCACATTCATGGACAA+AGG 0.632479 2.2:-66168671 MS.gene43593:intron
ATACAAATGACCAAGTTGCA+GGG 0.634663 2.2:-66166526 MS.gene43593:intron
CTTAGCACCAGATTCTGTAG+TGG 0.636413 2.2:+66168474 None:intergenic
AGATATCAATTCATTCAATG+AGG 0.653803 2.2:-66167413 MS.gene43593:intron
TGAATCAGAGAAGAATCCAA+AGG 0.659518 2.2:-66169477 MS.gene43593:CDS
CATATCTGATGAACTATACG+AGG 0.661127 2.2:-66167626 MS.gene43593:intron
TTGTTGATGATTGGAGACAG+TGG 0.718131 2.2:-66166550 MS.gene43593:CDS
TCGTCACGCCACTCACATAG+CGG 0.718285 2.2:+66167271 None:intergenic
GTTACTCGACAACTTAAGGG+TGG 0.723170 2.2:+66167175 None:intergenic
AGTGGCATAAGAGAAGCCTG+TGG 0.736747 2.2:+66168492 None:intergenic
TGTGCAAGTATAGATACACA+AGG 0.750084 2.2:-66165919 MS.gene43593:intron
ACGATACTGAAAATTCTCCG+AGG 0.753318 2.2:-66167252 MS.gene43593:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAAATTAACTTAAAAAT+AGG + chr2.2:66169104-66169123 None:intergenic 10.0%
!! CAAAAAAATAGTATAATAAT+TGG - chr2.2:66166391-66166410 MS.gene43593:intron 10.0%
!! CAAAAATTAACTTAAAAATA+GGG + chr2.2:66169103-66169122 None:intergenic 10.0%
!! TATTATAGTCATTATATATA+GGG - chr2.2:66168982-66169001 MS.gene43593:intron 10.0%
!! TTATTAACAAAAATTTCAAT+TGG + chr2.2:66166141-66166160 None:intergenic 10.0%
!! TTATTATAGTCATTATATAT+AGG - chr2.2:66168981-66169000 MS.gene43593:intron 10.0%
!!! AAGTTTTTTTTAATCTATTA+TGG - chr2.2:66165881-66165900 MS.gene43593:intron 10.0%
!!! TTAAAAAATTCTAGTTTTTT+GGG + chr2.2:66169180-66169199 None:intergenic 10.0%
!! AAACAAATATTAATTCAACT+TGG + chr2.2:66169523-66169542 None:intergenic 15.0%
!! AAGACATTCAAATTAAAATT+AGG - chr2.2:66166613-66166632 MS.gene43593:CDS 15.0%
!! ATCATGGTTATTATATATAA+GGG + chr2.2:66169490-66169509 None:intergenic 15.0%
!! GTTAATAAAGAAAAATGAAT+GGG - chr2.2:66166153-66166172 MS.gene43593:intron 15.0%
!! TAAAAATTTATGGAAAACAA+AGG + chr2.2:66168562-66168581 None:intergenic 15.0%
!! TATAAATAAAATCTTTGTAG+TGG - chr2.2:66167198-66167217 MS.gene43593:CDS 15.0%
!! TATAATTTCTTATGGAAAAT+TGG - chr2.2:66168807-66168826 MS.gene43593:intron 15.0%
!! TATCATGGTTATTATATATA+AGG + chr2.2:66169491-66169510 None:intergenic 15.0%
!! TGTTAATAAAGAAAAATGAA+TGG - chr2.2:66166152-66166171 MS.gene43593:intron 15.0%
!!! ATGAATTTTTATATGTTTGA+CGG + chr2.2:66169232-66169251 None:intergenic 15.0%
!!! GTTAAAAAATTCTAGTTTTT+TGG + chr2.2:66169181-66169200 None:intergenic 15.0%
!!! TAAAAAATTCTAGTTTTTTG+GGG + chr2.2:66169179-66169198 None:intergenic 15.0%
!!! TATTGAAGAAAAATTTTAGA+GGG + chr2.2:66169056-66169075 None:intergenic 15.0%
!!! TTATTGAAGAAAAATTTTAG+AGG + chr2.2:66169057-66169076 None:intergenic 15.0%
!!! TTATTTTTAACACATGATTA+TGG + chr2.2:66169630-66169649 None:intergenic 15.0%
!!! TTTTATATTTACAATAACAG+TGG - chr2.2:66168830-66168849 MS.gene43593:intron 15.0%
!! AATGATTGATCAAAATTTCA+GGG - chr2.2:66169672-66169691 MS.gene43593:CDS 20.0%
!! AGTTATTCATATTTCCTAAT+GGG - chr2.2:66168419-66168438 MS.gene43593:intron 20.0%
!! ATAACATTTCATAATTGATC+TGG + chr2.2:66167736-66167755 None:intergenic 20.0%
!! ATAATTTCTTGAACAATTAC+CGG + chr2.2:66167296-66167315 None:intergenic 20.0%
!! ATTGAATGAATTGATATCTT+TGG + chr2.2:66168054-66168073 None:intergenic 20.0%
!! ATTGTTCAAGAAATTATACA+AGG - chr2.2:66167299-66167318 MS.gene43593:CDS 20.0%
!! ATTTCATTTGATTGAAACTT+TGG + chr2.2:66167236-66167255 None:intergenic 20.0%
!! TAGATATAATTCATCTTTGT+TGG - chr2.2:66166589-66166608 MS.gene43593:CDS 20.0%
!! TATAATTATAATGCAGCAAA+TGG + chr2.2:66168340-66168359 None:intergenic 20.0%
!! TCCTTAAAAATTCATATGTT+TGG + chr2.2:66167043-66167062 None:intergenic 20.0%
!! TGAATGTGGATAAAAATTTA+TGG + chr2.2:66168572-66168591 None:intergenic 20.0%
!!! AAAAAATTCTAGTTTTTTGG+GGG + chr2.2:66169178-66169197 None:intergenic 20.0%
!!! AAATTTTCAATCACTACTTT+TGG - chr2.2:66169076-66169095 MS.gene43593:intron 20.0%
!!! AAATTTTCTATCTTCACTTT+TGG + chr2.2:66169383-66169402 None:intergenic 20.0%
!!! AACATATGAATTTTTAAGGA+AGG - chr2.2:66167043-66167062 MS.gene43593:CDS 20.0%
!!! AATATGAATAACTCTAAAAG+TGG + chr2.2:66168412-66168431 None:intergenic 20.0%
!!! ACCAAACATATGAATTTTTA+AGG - chr2.2:66167039-66167058 MS.gene43593:CDS 20.0%
!!! ACTAGTCTTATAATTTCTTA+TGG - chr2.2:66168799-66168818 MS.gene43593:intron 20.0%
!!! ATAAGGGTTAAATATGTTTT+TGG + chr2.2:66169474-66169493 None:intergenic 20.0%
!!! ATAGCATAATTTTCGTTATT+TGG + chr2.2:66167181-66167200 None:intergenic 20.0%
!!! ATATGAGTTTACTTTAAAAG+AGG - chr2.2:66169355-66169374 MS.gene43593:intron 20.0%
!!! ATGAAATGTTATTTTCTTCA+GGG - chr2.2:66167744-66167763 MS.gene43593:intron 20.0%
!!! ATTTATAAGATTTTTTCCCT+TGG - chr2.2:66166916-66166935 MS.gene43593:intron 20.0%
!!! CATATTCATATTTGTTGTTA+AGG - chr2.2:66165918-66165937 MS.gene43593:intron 20.0%
!!! CATTAAGTTGATCAAAAAAT+CGG + chr2.2:66167622-66167641 None:intergenic 20.0%
!!! GAAAAGTTGGTATATTTATA+GGG - chr2.2:66169447-66169466 MS.gene43593:CDS 20.0%
!!! GAAAAGTTGGTATATTTATA+GGG + chr2.2:66169466-66169447 None:intergenic 20.0%
!!! TATGAAATGTTATTTTCTTC+AGG - chr2.2:66167743-66167762 MS.gene43593:intron 20.0%
!!! TATGAGTTTACTTTAAAAGA+GGG - chr2.2:66169356-66169375 MS.gene43593:intron 20.0%
!!! TGAAGATAGAAAATTTTTGA+GGG - chr2.2:66169387-66169406 MS.gene43593:intron 20.0%
!!! TTAACTTTTGCCAAATATAT+CGG + chr2.2:66166226-66166245 None:intergenic 20.0%
! AACATGTCAAAACATTCTTT+AGG + chr2.2:66169726-66169745 None:intergenic 25.0%
! AACGAAATAATCAATCTTGA+AGG + chr2.2:66167701-66167720 None:intergenic 25.0%
! AATAAAATCTTTGTAGTGGT+TGG - chr2.2:66167202-66167221 MS.gene43593:CDS 25.0%
! AATTGAACTGTAAATGTTAC+AGG - chr2.2:66166720-66166739 MS.gene43593:intron 25.0%
! ACTTGGTCATAAATTTATCA+TGG + chr2.2:66169506-66169525 None:intergenic 25.0%
! AGATATCAATTCATTCAATG+AGG - chr2.2:66168055-66168074 MS.gene43593:intron 25.0%
! CAATGATTGATCAAAATTTC+AGG - chr2.2:66169671-66169690 MS.gene43593:CDS 25.0%
! CATGTAAATCTAAGTAGAAA+AGG - chr2.2:66168718-66168737 MS.gene43593:CDS 25.0%
! GAAGGTAATTTAAACAATGA+GGG - chr2.2:66169604-66169623 MS.gene43593:intron 25.0%
! GAGTTATTCATATTTCCTAA+TGG - chr2.2:66168418-66168437 MS.gene43593:intron 25.0%
! GATATAAGTATAGCTATGTA+TGG + chr2.2:66167913-66167932 None:intergenic 25.0%
! TCAAATGAAATTTACATTGC+TGG - chr2.2:66167245-66167264 MS.gene43593:CDS 25.0%
! TGAAGGTAATTTAAACAATG+AGG - chr2.2:66169603-66169622 MS.gene43593:intron 25.0%
! TGTAAAGAAATTTACTGATG+TGG - chr2.2:66166677-66166696 MS.gene43593:intron 25.0%
! TGTTTGTAGGAAATGAAAAA+GGG - chr2.2:66167429-66167448 MS.gene43593:CDS 25.0%
! TTAAAAAGGAGGAATACAAT+GGG - chr2.2:66166754-66166773 MS.gene43593:CDS 25.0%
! TTACAAATTCACTTGTTTGT+AGG - chr2.2:66167416-66167435 MS.gene43593:CDS 25.0%
! TTGTTAGTACTTATGTCAAT+CGG - chr2.2:66167151-66167170 MS.gene43593:intron 25.0%
! TTGTTTGTAGGAAATGAAAA+AGG - chr2.2:66167428-66167447 MS.gene43593:CDS 25.0%
! TTTAAAAAGGAGGAATACAA+TGG - chr2.2:66166753-66166772 MS.gene43593:CDS 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG - chr2.2:66169415-66169434 MS.gene43593:CDS 25.0%
!! ACAAAGTTTAGTTCAACTTA+TGG - chr2.2:66165717-66165736 MS.gene43593:CDS 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG - chr2.2:66169416-66169435 MS.gene43593:CDS 25.0%
!! CAAAGTTTAGTTCAACTTAT+GGG - chr2.2:66165718-66165737 MS.gene43593:CDS 25.0%
!! CTTTTCAAATTGTTCACTTT+TGG - chr2.2:66166100-66166119 MS.gene43593:intron 25.0%
!! GTGAAGATAGAAAATTTTTG+AGG - chr2.2:66169386-66169405 MS.gene43593:intron 25.0%
!! TTTGGAACATAAAACATTTC+TGG + chr2.2:66168036-66168055 None:intergenic 25.0%
!!! AATGTTTTGACATGTTTAGT+AGG - chr2.2:66169730-66169749 MS.gene43593:CDS 25.0%
!!! ATATTTGTTGTTAAGGTATG+TGG - chr2.2:66165925-66165944 MS.gene43593:intron 25.0%
!!! CGAAAAGTTGGTATATTTAT+AGG - chr2.2:66169446-66169465 MS.gene43593:CDS 25.0%
!!! CGAAAAGTTGGTATATTTAT+AGG + chr2.2:66169465-66169446 None:intergenic 25.0%
!!! TATTTGTTGTTAAGGTATGT+GGG - chr2.2:66165926-66165945 MS.gene43593:intron 25.0%
AAATCAGGTGACAAAAAATG+TGG + chr2.2:66166836-66166855 None:intergenic 30.0%
ACATGATAATCATAGCTGTT+AGG + chr2.2:66168703-66168722 None:intergenic 30.0%
AGCATTATGACGTATATTGT+AGG + chr2.2:66168674-66168693 None:intergenic 30.0%
ATAAGTATAGCTATGTATGG+AGG + chr2.2:66167910-66167929 None:intergenic 30.0%
CATGATAATCATAGCTGTTA+GGG + chr2.2:66168702-66168721 None:intergenic 30.0%
CATTATATTTGTAGATTCGC+CGG - chr2.2:66166950-66166969 MS.gene43593:intron 30.0%
TATACAAGGTACAAAACTCA+TGG - chr2.2:66167313-66167332 MS.gene43593:intron 30.0%
TCGCTGCAAAAAAATTGTAA+TGG - chr2.2:66167990-66168009 MS.gene43593:intron 30.0%
TGATACTTTAAATGCTAACC+TGG + chr2.2:66167475-66167494 None:intergenic 30.0%
TGCAATCAAGTTCTTATGTA+AGG + chr2.2:66168613-66168632 None:intergenic 30.0%
TTCTTAATTAGAGATCTCCT+CGG + chr2.2:66168236-66168255 None:intergenic 30.0%
! AGAGTTGTCACATATTCTTT+TGG - chr2.2:66166482-66166501 MS.gene43593:intron 30.0%
! AGTTCATCAGTGTTTTCTTA+TGG + chr2.2:66168782-66168801 None:intergenic 30.0%
! GAATAACTCTAAAAGTGGAA+AGG + chr2.2:66168407-66168426 None:intergenic 30.0%
! GCAATTTGAGATTTGTATTG+AGG + chr2.2:66168274-66168293 None:intergenic 30.0%
!! ACTATTCCATTGTTGATGAT+TGG - chr2.2:66168909-66168928 MS.gene43593:intron 30.0%
!! ATATTTCCTAATGGGTTTCT+GGG - chr2.2:66168427-66168446 MS.gene43593:CDS 30.0%
!! GAATGCAAAAGTTCTAAATC+AGG + chr2.2:66166851-66166870 None:intergenic 30.0%
!!! ATAAGATTTTTTCCCTTGGT+AGG - chr2.2:66166920-66166939 MS.gene43593:intron 30.0%
AAATCTTCACCAACCATAAG+GGG + chr2.2:66166568-66166587 None:intergenic 35.0%
AAGTATCACACTCTCATGTT+AGG - chr2.2:66167487-66167506 MS.gene43593:intron 35.0%
AGAAATCTTCACCAACCATA+AGG + chr2.2:66166570-66166589 None:intergenic 35.0%
AGGTCCACTTGAGTTTAAAA+AGG - chr2.2:66166740-66166759 MS.gene43593:CDS 35.0%
ATAATGTTACTCACCTACCA+AGG + chr2.2:66166936-66166955 None:intergenic 35.0%
ATACAAATGACCAAGTTGCA+GGG - chr2.2:66168942-66168961 MS.gene43593:intron 35.0%
ATATCGAATTGACACATGGA+TGG + chr2.2:66166200-66166219 None:intergenic 35.0%
CAAAGCAATACACATACGTA+AGG - chr2.2:66168469-66168488 MS.gene43593:CDS 35.0%
CAATGGTGTAACAACTAGAA+AGG - chr2.2:66167779-66167798 MS.gene43593:intron 35.0%
CATATCTGATGAACTATACG+AGG - chr2.2:66167842-66167861 MS.gene43593:intron 35.0%
CTAACCTGGAGATTTATCAA+TGG + chr2.2:66167461-66167480 None:intergenic 35.0%
GAAATCTTCACCAACCATAA+GGG + chr2.2:66166569-66166588 None:intergenic 35.0%
GAGATTTGTATTGAGGAACT+TGG + chr2.2:66168267-66168286 None:intergenic 35.0%
GATTGATCAAAATTTCAGGG+TGG - chr2.2:66169675-66169694 MS.gene43593:CDS 35.0%
GCTTCAAGATCAAATTAGGT+AGG + chr2.2:66166782-66166801 None:intergenic 35.0%
TAATGTTACTCACCTACCAA+GGG + chr2.2:66166935-66166954 None:intergenic 35.0%
TCCACTTGAGTTTAAAAAGG+AGG - chr2.2:66166743-66166762 MS.gene43593:CDS 35.0%
TCTATAGTGAAGTTTCTTCC+TGG - chr2.2:66165804-66165823 MS.gene43593:intron 35.0%
TGAATCAGAGAAGAATCCAA+AGG - chr2.2:66165991-66166010 MS.gene43593:intron 35.0%
TGACATGACAATTCCATTCT+TGG - chr2.2:66168859-66168878 MS.gene43593:intron 35.0%
TGTCAAAACATTCTTTAGGC+CGG + chr2.2:66169722-66169741 None:intergenic 35.0%
TGTGCAAGTATAGATACACA+AGG - chr2.2:66169549-66169568 MS.gene43593:intron 35.0%
TGTGGCTTCAAGATCAAATT+AGG + chr2.2:66166786-66166805 None:intergenic 35.0%
TTAGTAGGTGGTTATCTAAG+AGG - chr2.2:66169745-66169764 MS.gene43593:CDS 35.0%
TTTCTTCAGGGATACTTGTT+GGG - chr2.2:66167756-66167775 MS.gene43593:intron 35.0%
TTTGAGGGACTAAAAGTTGA+AGG - chr2.2:66169402-66169421 MS.gene43593:intron 35.0%
! ATTTTGAATACCCTGCAACT+TGG + chr2.2:66168955-66168974 None:intergenic 35.0%
! GTTTAGTTCAACTTATGGGA+AGG - chr2.2:66165722-66165741 MS.gene43593:CDS 35.0%
! TATTTTTCACAGCTCACATC+AGG - chr2.2:66168149-66168168 MS.gene43593:intron 35.0%
! TCCTCCTTTTTAAACTCAAG+TGG + chr2.2:66166747-66166766 None:intergenic 35.0%
! TCGATATGCACCGATATATT+TGG - chr2.2:66166213-66166232 MS.gene43593:intron 35.0%
! TTTTCTTCAGGGATACTTGT+TGG - chr2.2:66167755-66167774 MS.gene43593:intron 35.0%
!! CATATTTCCTAATGGGTTTC+TGG - chr2.2:66168426-66168445 MS.gene43593:CDS 35.0%
!! GTTTTGACATGTTTAGTAGG+TGG - chr2.2:66169733-66169752 MS.gene43593:CDS 35.0%
!! TCTGGTCTTATGCTTGAAAT+TGG - chr2.2:66166064-66166083 MS.gene43593:intron 35.0%
AACTTGATTGCATGTGTTGC+AGG - chr2.2:66168621-66168640 MS.gene43593:intron 40.0%
AAGCGTTACTCGACAACTTA+AGG + chr2.2:66168300-66168319 None:intergenic 40.0%
AAGTTTCTTCCTGGATTCCA+AGG - chr2.2:66165813-66165832 MS.gene43593:intron 40.0%
AATCCCATTTGCTCATGGAA+TGG - chr2.2:66167815-66167834 MS.gene43593:intron 40.0%
ACATTCATGGACAAAGGTTC+GGG - chr2.2:66166803-66166822 MS.gene43593:CDS 40.0%
ACGATACTGAAAATTCTCCG+AGG - chr2.2:66168216-66168235 MS.gene43593:CDS 40.0%
ACTTGATTGCATGTGTTGCA+GGG - chr2.2:66168622-66168641 MS.gene43593:intron 40.0%
AGCGTTACTCGACAACTTAA+GGG + chr2.2:66168299-66168318 None:intergenic 40.0%
AGGATGATCCTCTCATACTA+TGG - chr2.2:66166011-66166030 MS.gene43593:intron 40.0%
AGTATGAGAGGATCATCCTT+TGG + chr2.2:66166010-66166029 None:intergenic 40.0%
ATCCCATTTGCTCATGGAAT+GGG - chr2.2:66167816-66167835 MS.gene43593:intron 40.0%
ATTGCTGGTGATTCATACTC+TGG - chr2.2:66167260-66167279 MS.gene43593:CDS 40.0%
CACATTCATGGACAAAGGTT+CGG - chr2.2:66166802-66166821 MS.gene43593:CDS 40.0%
CATACAAATGACCAAGTTGC+AGG - chr2.2:66168941-66168960 MS.gene43593:intron 40.0%
CGAACCATTGATAAATCTCC+AGG - chr2.2:66167454-66167473 MS.gene43593:CDS 40.0%
CTGTCTCCAATCATCAACAA+TGG + chr2.2:66168918-66168937 None:intergenic 40.0%
GAGGGACTAAAACGAAAAGT+TGG - chr2.2:66169434-66169453 MS.gene43593:CDS 40.0%
GAGGGACTAAAACGAAAAGT+TGG + chr2.2:66169453-66169434 None:intergenic 40.0%
GATGACATTTGCAACTGTGA+AGG - chr2.2:66169586-66169605 MS.gene43593:intron 40.0%
GGTTTCAAGCACAAAAGGAA+GGG + chr2.2:66165841-66165860 None:intergenic 40.0%
GTGCATATCGAATTGACACA+TGG + chr2.2:66166204-66166223 None:intergenic 40.0%
GTTATCTAAGAGGCCTTTGT+AGG - chr2.2:66169755-66169774 MS.gene43593:CDS 40.0%
GTTTCAAGCACAAAAGGAAG+GGG + chr2.2:66165840-66165859 None:intergenic 40.0%
TATGCAATCCCATTTGCTCA+TGG - chr2.2:66167810-66167829 MS.gene43593:intron 40.0%
TCTTATGCCACTACAGAATC+TGG - chr2.2:66166987-66167006 MS.gene43593:intron 40.0%
TGATCAAAATTTCAGGGTGG+AGG - chr2.2:66169678-66169697 MS.gene43593:CDS 40.0%
TGATCTTGAAGCCACATTCA+TGG - chr2.2:66166790-66166809 MS.gene43593:CDS 40.0%
TGTAAAGCCATGAACTAACC+CGG + chr2.2:66165862-66165881 None:intergenic 40.0%
TTGTTAAGGTATGTGGGAGT+TGG - chr2.2:66165932-66165951 MS.gene43593:intron 40.0%
! ATACTCTGGTATTGCTGTTC+CGG - chr2.2:66167274-66167293 MS.gene43593:CDS 40.0%
! CAAAAAATCGGTGCTTGTGT+CGG + chr2.2:66167610-66167629 None:intergenic 40.0%
! CTTCCTTTTGTGCTTGAAAC+CGG - chr2.2:66165840-66165859 MS.gene43593:intron 40.0%
! TTCCTTTTGTGCTTGAAACC+GGG - chr2.2:66165841-66165860 MS.gene43593:intron 40.0%
!! TGCAGGGAAGTATTGGAAAA+TGG - chr2.2:66168638-66168657 MS.gene43593:intron 40.0%
!! TGCTTTCAAGTTGCAACATG+TGG - chr2.2:66165780-66165799 MS.gene43593:intron 40.0%
!! TTGTTGATGATTGGAGACAG+TGG - chr2.2:66168918-66168937 MS.gene43593:intron 40.0%
AACCCGGTTTCAAGCACAAA+AGG + chr2.2:66165846-66165865 None:intergenic 45.0%
AGTCCCATTCCATGAGCAAA+TGG + chr2.2:66167822-66167841 None:intergenic 45.0%
CATGCTTGTGTTGCCAAGAA+TGG + chr2.2:66168875-66168894 None:intergenic 45.0%
CATTAGCCCAGAAACCCATT+AGG + chr2.2:66168436-66168455 None:intergenic 45.0%
CATTCTTGGCAACACAAGCA+TGG - chr2.2:66168873-66168892 MS.gene43593:intron 45.0%
CCAACCATAAGGGGTTAACA+AGG + chr2.2:66166559-66166578 None:intergenic 45.0%
CCAGTTAGCCATAGTATGAG+AGG + chr2.2:66166022-66166041 None:intergenic 45.0%
CCTCTCATACTATGGCTAAC+TGG - chr2.2:66166019-66166038 MS.gene43593:intron 45.0%
CCTTGTTAACCCCTTATGGT+TGG - chr2.2:66166556-66166575 MS.gene43593:CDS 45.0%
CGAACCTTTGTCCATGAATG+TGG + chr2.2:66166804-66166823 None:intergenic 45.0%
CGGTTTCAAGCACAAAAGGA+AGG + chr2.2:66165842-66165861 None:intergenic 45.0%
CTCATACTATGGCTAACTGG+TGG - chr2.2:66166022-66166041 MS.gene43593:intron 45.0%
CTTAGCACCAGATTCTGTAG+TGG + chr2.2:66166997-66167016 None:intergenic 45.0%
GAAGCCACATTCATGGACAA+AGG - chr2.2:66166797-66166816 MS.gene43593:CDS 45.0%
GCGTATATCTCGAGCTTCAT+AGG + chr2.2:66167942-66167961 None:intergenic 45.0%
GTCCCATTCCATGAGCAAAT+GGG + chr2.2:66167821-66167840 None:intergenic 45.0%
GTTACTCGACAACTTAAGGG+TGG + chr2.2:66168296-66168315 None:intergenic 45.0%
TAAGAGAAGCCTGTGGAAAC+CGG + chr2.2:66166972-66166991 None:intergenic 45.0%
! CATGTGTTGCAGGGAAGTAT+TGG - chr2.2:66168631-66168650 MS.gene43593:intron 45.0%
! TTTTAGACCCGCTATGTGAG+TGG - chr2.2:66168189-66168208 MS.gene43593:CDS 45.0%
!! AACAAGGCTAGCCTACATGA+GGG + chr2.2:66166543-66166562 None:intergenic 45.0%
!! CTAGCCTTGTTAACCCCTTA+TGG - chr2.2:66166552-66166571 MS.gene43593:CDS 45.0%
!! CTTGAAACCGGGTTAGTTCA+TGG - chr2.2:66165852-66165871 MS.gene43593:intron 45.0%
!! TAACAAGGCTAGCCTACATG+AGG + chr2.2:66166544-66166563 None:intergenic 45.0%
!!! AAAAAATTCTAATTTTTTTT+TGG - chr2.2:66169271-66169290 MS.gene43593:intron 5.0%
AGTGGCATAAGAGAAGCCTG+TGG + chr2.2:66166979-66166998 None:intergenic 50.0%
CTCAACTTCCTCCCTCATGT+AGG - chr2.2:66166529-66166548 MS.gene43593:CDS 50.0%
! CAGGGATACTTGTTGGGCAA+TGG - chr2.2:66167762-66167781 MS.gene43593:intron 50.0%
! TGCTTGTGTCGGTGCTTTCT+AGG + chr2.2:66167599-66167618 None:intergenic 50.0%
!! TGTGGGAGTTGGTGAGAAAG+AGG - chr2.2:66165943-66165962 MS.gene43593:intron 50.0%
CCAGACAAAGCAGAGCAACC+AGG + chr2.2:66166049-66166068 None:intergenic 55.0%
CCTGGTTGCTCTGCTTTGTC+TGG - chr2.2:66166046-66166065 MS.gene43593:intron 55.0%
GCACAAAAGGAAGGGGTCCT+TGG + chr2.2:66165833-66165852 None:intergenic 55.0%
TCGTCACGCCACTCACATAG+CGG + chr2.2:66168200-66168219 None:intergenic 55.0%
TGTGCGACGTCGCATGAATG+TGG + chr2.2:66168586-66168605 None:intergenic 55.0%
! CTATGGCTAACTGGTGGACC+TGG - chr2.2:66166028-66166047 MS.gene43593:intron 55.0%
!! AAGGCTAGCCTACATGAGGG+AGG + chr2.2:66166540-66166559 None:intergenic 55.0%
!! GTAGATTCGCCGGTTTCCAC+AGG - chr2.2:66166960-66166979 MS.gene43593:intron 55.0%
CGTCACGCCACTCACATAGC+GGG + chr2.2:66168199-66168218 None:intergenic 60.0%
GGAAGGGGTCCTTGGAATCC+AGG + chr2.2:66165825-66165844 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 66165714 66169776 66165714 ID=MS.gene43593
chr2.2 mRNA 66165714 66169776 66165714 ID=MS.gene43593.t1;Parent=MS.gene43593
chr2.2 exon 66169628 66169776 66169628 ID=MS.gene43593.t1.exon1;Parent=MS.gene43593.t1
chr2.2 CDS 66169628 66169776 66169628 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
chr2.2 exon 66169405 66169550 66169405 ID=MS.gene43593.t1.exon2;Parent=MS.gene43593.t1
chr2.2 CDS 66169405 66169550 66169405 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
chr2.2 exon 66168672 66168748 66168672 ID=MS.gene43593.t1.exon3;Parent=MS.gene43593.t1
chr2.2 CDS 66168672 66168748 66168672 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
chr2.2 exon 66168426 66168548 66168426 ID=MS.gene43593.t1.exon4;Parent=MS.gene43593.t1
chr2.2 CDS 66168426 66168548 66168426 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
chr2.2 exon 66168170 66168272 66168170 ID=MS.gene43593.t1.exon5;Parent=MS.gene43593.t1
chr2.2 CDS 66168170 66168272 66168170 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
chr2.2 exon 66168015 66168052 66168015 ID=MS.gene43593.t1.exon6;Parent=MS.gene43593.t1
chr2.2 CDS 66168015 66168052 66168015 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
chr2.2 exon 66167627 66167725 66167627 ID=MS.gene43593.t1.exon7;Parent=MS.gene43593.t1
chr2.2 CDS 66167627 66167725 66167627 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
chr2.2 exon 66167414 66167500 66167414 ID=MS.gene43593.t1.exon8;Parent=MS.gene43593.t1
chr2.2 CDS 66167414 66167500 66167414 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
chr2.2 exon 66167180 66167329 66167180 ID=MS.gene43593.t1.exon9;Parent=MS.gene43593.t1
chr2.2 CDS 66167180 66167329 66167180 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
chr2.2 exon 66167000 66167071 66167000 ID=MS.gene43593.t1.exon10;Parent=MS.gene43593.t1
chr2.2 CDS 66167000 66167071 66167000 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
chr2.2 exon 66166729 66166847 66166729 ID=MS.gene43593.t1.exon11;Parent=MS.gene43593.t1
chr2.2 CDS 66166729 66166847 66166729 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
chr2.2 exon 66166527 66166640 66166527 ID=MS.gene43593.t1.exon12;Parent=MS.gene43593.t1
chr2.2 CDS 66166527 66166640 66166527 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
chr2.2 exon 66165883 66165928 66165883 ID=MS.gene43593.t1.exon13;Parent=MS.gene43593.t1
chr2.2 CDS 66165883 66165928 66165883 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
chr2.2 exon 66165714 66165797 66165714 ID=MS.gene43593.t1.exon14;Parent=MS.gene43593.t1
chr2.2 CDS 66165714 66165797 66165714 ID=cds.MS.gene43593.t1;Parent=MS.gene43593.t1
Gene Sequence

>MS.gene43593

ATGACAAAGTTTAGTTCAACTTATGGGAAGGTTCTTCTTTCTGTTTTTCTTTTATTATCACATATTTGCTTTCAAGTTGCAACATGTGGCTCTATAGTGAAGTTTCTTCCTGGATTCCAAGGACCCCTTCCTTTTGTGCTTGAAACCGGGTTAGTTCATGGCTTTACAAGTTTTTTTTAATCTATTATGGTATTTAAATAATTTCATATTCATATTTGTTGTTAAGGTATGTGGGAGTTGGTGAGAAAGAGGATGTGCAAGTGTTTTACTATTTCATTGAATCAGAGAAGAATCCAAAGGATGATCCTCTCATACTATGGCTAACTGGTGGACCTGGTTGCTCTGCTTTGTCTGGTCTTATGCTTGAAATTGGTAATTGCAACTTGCTTTTCAAATTGTTCACTTTTGGATAGTCTTACAATAACCAATTGAAATTTTTGTTAATAAAGAAAAATGAATGGGTAAATGTTAGTGAGAATTTTTCCATCCATGTGTCAATTCGATATGCACCGATATATTTGGCAAAAGTTAATTTTTGAAAAAAAAATTTATTTTCTATCATATAAAAAGTTATATTAATTAAATTATATTTATCAAAGTGTATGCGAAATAAATTTAATTTTTTTCAATTTGTTTTCTCGTAATAGTTAATATTGAATTATTTTACGATAAAATGTCAAAAAAATAGTATAATAATTGGAAATAATGAAATGATTTTTTTTCTAATGAAATATGTGACAACTCTATGATTTTTTACTAAAAATTTATAGAGTTGTCACATATTCTTTTGGTTTATATTTAATATTTCGCATTCTCTCAACTTCCTCCCTCATGTAGGCTAGCCTTGTTAACCCCTTATGGTTGGTGAAGATTTCTAGATATAATTCATCTTTGTTGGAAAGACATTCAAATTAAAATTAGGAAGATCAATAGACACTGCAATGATACTAATTGAAGTGTCAATGTAAAGAAATTTACTGATGTGGTAAAATGATACTCATTGAATAATTGAACTGTAAATGTTACAGGTCCACTTGAGTTTAAAAAGGAGGAATACAATGGGAGCCTACCTAATTTGATCTTGAAGCCACATTCATGGACAAAGGTTCGGGTCATAGTCCACATTTTTTGTCACCTGATTTAGAACTTTTGCATTCAATGTATTTATCAAATTCTTTTAAATTATATATTTATTTACTAGTATTTATAAGATTTTTTCCCTTGGTAGGTGAGTAACATTATATTTGTAGATTCGCCGGTTTCCACAGGCTTCTCTTATGCCACTACAGAATCTGGTGCTAAGCGAAGCGACTCGATTCTAGTTCACCAAACATATGAATTTTTAAGGAAGGTATACATTTCACAATAATCACTTTTTTTATGTTTTAAAACTTTGTTTTTTTTATTTTATTTTAAAAAGAAGTGTGTTTTTTAACTTTGTTAGTACTTATGTCAATCGGTCAACCAAATAACGAAAATTATGCTATAAATAAAATCTTTGTAGTGGTTGGTTGATCATCCAAAGTTTCAATCAAATGAAATTTACATTGCTGGTGATTCATACTCTGGTATTGCTGTTCCGGTAATTGTTCAAGAAATTATACAAGGTACAAAACTCATGGCTCAATTATGTATTGCAGAACGATTGTGTTACAATTGAATTGATTTACTGTATGTTGTAATTTTAATTAAGTTTAATTATTTACAAATTCACTTGTTTGTAGGAAATGAAAAAGGGATCGAACCATTGATAAATCTCCAGGTTAGCATTTAAAGTATCACACTCTCATGTTAGGAATGCATTTTTTCAACTAGTTCTATAAATTACAAATGTACATTGTATGTTTCAATAATGTGAAAATTGCATTTCAACTATTGAAAACCTAGAAAGCACCGACACAAGCACCGATTTTTTGATCAACTTAATGTTTCGATCAAATAGATTATGCATGACGTGTAATAAAACAATCGACTACATACAATACCTTCAAGATTGATTATTTCGTTATATAACAGCTTCCAGATCAATTATGAAATGTTATTTTCTTCAGGGATACTTGTTGGGCAATGGTGTAACAACTAGAAAGGAAAAAAATTATGCAATCCCATTTGCTCATGGAATGGGACTCATATCTGATGAACTATACGAGGTAACTGAATTATACTTTCACAGTATCTAATTGAGTTATAATTCCTCCATACATAGCTATACTTATATCGAAAAACCTATGAAGCTCGAGATATACGCTAACTAATTGATATCTGCGATTTTGTAGTCGCTGCAAAAAAATTGTAATGGAGACTATGTAAATGCAGAGACCAGAAATGTTTTATGTTCCAAAGATATCAATTCATTCAATGAGGTATATGTTTATTTTATTTTTGTCAAATTTCTCGAGTAGAATTACTAGTCACAAAAATGAACATCATTCTTCTATTTTTCACAGCTCACATCAGGACTTAATACAGCACATATTTTAGACCCGCTATGTGAGTGGCGTGACGATACTGAAAATTCTCCGAGGAGATCTCTAATTAAGAATTATTTCTCCAAGTTCCTCAATACAAATCTCAAATTGCCACCCTTAAGTTGTCGAGTAACGCTTCTTACTATATATATATACCATTTGCTGCATTATAATTATAAATATATATAAAGCTCAATTTTTTTGTTAACTTGATGTTTGCTTCCTTTCCACTTTTAGAGTTATTCATATTTCCTAATGGGTTTCTGGGCTAATGATGATAATGTTCGCAAAGCAATACACATACGTAAGGTATATTATTGATACATATATGCGAAAGTTGTAATTTCTTCATGCTATCTGTATATTCATATTTCACACCTTTGTTTTCCATAAATTTTTATCCACATTCATGCGACGTCGCACATAGTCCTTACATAAGAACTTGATTGCATGTGTTGCAGGGAAGTATTGGAAAATGGCATCGTTGCACCTACAATATACGTCATAATGCTGATATCCCTAACAGCTATGATTATCATGTAAATCTAAGTAGAAAAGGCATTCGTTCACTAATTTACAAGTCAGTAATCTTTCTCTCCATAAGAAAACACTGATGAACTAGTCTTATAATTTCTTATGGAAAATTGGTTTTATATTTACAATAACAGTGGCGATCATGACATGACAATTCCATTCTTGGCAACACAAGCATGGATAAGATCTTTGAACTATTCCATTGTTGATGATTGGAGACAGTGGCATACAAATGACCAAGTTGCAGGGTATTCAAAATTTCTAATTATTATAGTCATTATATATAGGGTTAAATATGTTTTTGATCCCTATAAATATACCAACTTTTCGTTTTAGTCCCTCTAAAATTTTTCTTCAATAAAATTTTCAATCACTACTTTTGGTCCCTATTTTTAAGTTAATTTTTGTATTTTGTAATGAAATTGTACAGAAATGTGTAGAATATTGTAAAAAAAAATTCCCCCAAAAAACTAGAATTTTTTAACAAAACATAAATTAATTATGAATTTTTAACCGTCAAACATATAAAAATTCATATTAAATTCATGTTTTGTTAAAAAATTCTAATTTTTTTTTGGAGAGATTATTACAATATTATGAATTTTTCTGCAAAATTTTATTCAAAAATATGTATTCTACATATGAGTTTACTTTAAAAGAGGGACCAAAAGTGAAGATAGAAAATTTTTGAGGGACTAAAAGTTGAAGGAAAATTTTAGAGGGACTAAAACGAAAAGTTGGTATATTTATAGGGACCAAAAACATATTTAACCCTTATATATAATAACCATGATAAATTTATGACCAAGTTGAATTAATATTTGTTTTAACTGTGTGCAAGTATAGATACACAAGGACTTACTCAAATCAGATGACATTTGCAACTGTGAAGGTAATTTAAACAATGAGGGCCATAATCATGTGTTAAAAATAATGACTAGTTTATTTACTCTATCAATGATTGATCAAAATTTCAGGGTGGAGGACACACAGCTCCAGAGTTCCGGCCTAAAGAATGTTTTGACATGTTTAGTAGGTGGTTATCTAAGAGGCCTTTGTAG
Protein sequence

>MS.gene43593.t1

MTKFSSTYGKVLLSVFLLLSHICFQVATCGSIVKFLPGFQGPLPFVLETGYVGVGEKEDVQVFYYFIESEKNPKDDPLILWLTGGPGCSALSGLMLEIGPLEFKKEEYNGSLPNLILKPHSWTKVSNIIFVDSPVSTGFSYATTESGAKRSDSILVHQTYEFLRKWLVDHPKFQSNEIYIAGDSYSGIAVPVIVQEIIQGNEKGIEPLINLQGYLLGNGVTTRKEKNYAIPFAHGMGLISDELYESLQKNCNGDYVNAETRNVLCSKDINSFNELTSGLNTAHILDPLCEWRDDTENSPRRSLIKNYFSKFLNTNLKLPPLSCRSYSYFLMGFWANDDNVRKAIHIRKGSIGKWHRCTYNIRHNADIPNSYDYHVNLSRKGIRSLIYNGDHDMTIPFLATQAWIRSLNYSIVDDWRQWHTNDQVAGYTRTYSNQMTFATVKGGGHTAPEFRPKECFDMFSRWLSKRPL