AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002688

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002688.t1 MTR_2g018590 94.030 469 27 1 1 468 1 469 0.0 925
MS.gene002688.t1 MTR_2g018600 86.354 469 63 1 1 468 1 469 0.0 838
MS.gene002688.t1 MTR_2g018630 74.667 450 105 3 20 468 24 465 0.0 704
MS.gene002688.t1 MTR_3g100420 64.882 467 154 4 12 468 30 496 0.0 627
MS.gene002688.t1 MTR_4g021520 62.806 449 160 5 21 468 28 470 0.0 580
MS.gene002688.t1 MTR_4g021510 62.247 445 161 5 21 464 27 465 0.0 569
MS.gene002688.t1 MTR_4g021500 61.174 443 165 5 21 462 28 464 0.0 564
MS.gene002688.t1 MTR_4g021500 59.259 432 149 6 39 468 481 887 1.35e-175 515
MS.gene002688.t1 MTR_4g021500 61.174 443 165 5 21 462 28 464 0.0 563
MS.gene002688.t1 MTR_4g021500 60.648 432 161 5 39 468 481 905 0.0 542
MS.gene002688.t1 MTR_4g021490 59.688 449 174 5 21 468 28 470 0.0 555
MS.gene002688.t1 MTR_4g021535 58.114 456 184 5 10 464 13 462 0.0 553
MS.gene002688.t1 MTR_2g018630 71.662 367 95 3 103 468 2 360 0.0 547
MS.gene002688.t1 MTR_4g021530 57.895 456 185 6 10 464 17 466 0.0 541
MS.gene002688.t1 MTR_4g021520 61.916 407 148 5 21 426 28 428 0.0 513
MS.gene002688.t1 MTR_4g021535 50.877 456 170 6 10 464 13 415 6.42e-160 459
MS.gene002688.t1 MTR_3g045770 43.791 459 236 5 11 464 9 450 1.91e-136 400
MS.gene002688.t1 MTR_2g009200 42.232 457 222 8 16 463 11 434 2.77e-125 372
MS.gene002688.t1 MTR_8g011350 39.034 497 264 8 1 464 1 491 1.44e-122 367
MS.gene002688.t1 MTR_7g051430 40.041 487 254 8 11 464 10 491 3.31e-122 365
MS.gene002688.t1 MTR_7g080180 39.718 496 256 10 11 468 8 498 6.54e-121 362
MS.gene002688.t1 MTR_8g011350 38.819 474 251 8 1 441 1 468 2.36e-111 337
MS.gene002688.t1 MTR_7g080180 38.987 454 234 10 11 426 8 456 1.03e-101 312
MS.gene002688.t1 MTR_7g051430 37.805 410 217 8 11 387 10 414 2.74e-85 268
MS.gene002688.t1 MTR_4g075150 32.051 468 292 14 13 468 9 462 1.17e-67 224
MS.gene002688.t1 MTR_4g075150 32.265 468 289 14 13 468 9 460 3.10e-67 223
MS.gene002688.t1 MTR_2g012860 30.538 465 290 15 18 468 27 472 7.29e-58 198
MS.gene002688.t1 MTR_4g122960 29.399 466 300 16 13 464 20 470 1.69e-57 197
MS.gene002688.t1 MTR_4g122950 29.399 466 302 16 13 464 20 472 5.97e-57 196
MS.gene002688.t1 MTR_2g063000 28.750 480 298 16 5 464 3 458 2.71e-56 194
MS.gene002688.t1 MTR_8g099930 29.916 478 261 16 24 468 25 461 3.31e-56 194
MS.gene002688.t1 MTR_5g072570 29.053 475 299 16 12 468 6 460 3.66e-56 194
MS.gene002688.t1 MTR_1g095540 29.936 471 292 15 12 466 5 453 4.64e-56 193
MS.gene002688.t1 MTR_2g062980 29.957 464 292 14 2 453 4 446 5.40e-56 193
MS.gene002688.t1 MTR_2g062990 29.526 464 294 15 2 453 4 446 7.85e-56 192
MS.gene002688.t1 MTR_2g012850 29.663 445 280 14 32 463 35 459 9.14e-56 192
MS.gene002688.t1 MTR_7g013790 29.241 448 279 13 36 468 39 463 2.29e-55 191
MS.gene002688.t1 MTR_3g434850 29.478 441 275 14 40 468 37 453 2.36e-55 191
MS.gene002688.t1 MTR_1g021010 32.648 438 255 15 33 463 76 480 1.11e-54 190
MS.gene002688.t1 MTR_3g079590 30.184 434 267 12 36 463 81 484 1.45e-54 190
MS.gene002688.t1 MTR_1g095560 30.042 476 291 17 9 466 2 453 1.71e-54 189
MS.gene002688.t1 MTR_2g081620 30.337 445 264 16 36 464 44 458 9.48e-54 187
MS.gene002688.t1 MTR_3g498755 28.243 478 275 13 22 468 4 444 1.43e-53 186
MS.gene002688.t1 MTR_4g089155 29.478 441 288 14 32 463 53 479 6.70e-53 185
MS.gene002688.t1 MTR_2g449850 29.167 432 262 14 51 466 56 459 3.72e-52 182
MS.gene002688.t1 MTR_3g079560 29.345 443 275 13 33 468 78 489 7.47e-52 182
MS.gene002688.t1 MTR_3g079570 29.398 432 272 12 36 463 82 484 7.63e-52 182
MS.gene002688.t1 MTR_3g034290 28.604 437 281 12 33 464 75 485 8.79e-52 182
MS.gene002688.t1 MTR_3g090130 29.556 450 282 14 32 468 28 455 1.20e-51 181
MS.gene002688.t1 MTR_0003s0560 29.091 440 271 13 33 463 74 481 1.90e-51 181
MS.gene002688.t1 MTR_1g020090 32.273 440 251 14 33 463 90 491 4.14e-51 181
MS.gene002688.t1 MTR_7g021130 28.302 477 294 14 11 468 9 456 9.22e-51 179
MS.gene002688.t1 MTR_3g090150 28.352 455 282 15 32 468 34 462 6.30e-50 177
MS.gene002688.t1 MTR_3g079600 28.833 437 278 13 33 463 76 485 4.53e-49 175
MS.gene002688.t1 MTR_1g095560 28.150 508 291 18 9 466 2 485 2.94e-48 173
MS.gene002688.t1 MTR_3g079620 28.859 447 274 14 36 468 81 497 9.64e-48 172
MS.gene002688.t1 MTR_1g021020 29.773 440 264 15 33 463 78 481 1.16e-47 171
MS.gene002688.t1 MTR_2g063000 28.211 475 276 15 5 464 3 427 2.76e-47 169
MS.gene002688.t1 MTR_2g095750 27.333 450 276 10 36 468 85 500 1.51e-46 169
MS.gene002688.t1 MTR_3g079630 28.539 445 268 15 36 463 81 492 1.61e-46 168
MS.gene002688.t1 MTR_4g057585 28.111 434 252 11 49 463 98 490 1.02e-45 166
MS.gene002688.t1 MTR_4g057595 28.111 434 252 11 49 463 99 491 1.16e-45 166
MS.gene002688.t1 MTR_4g057600 26.728 434 268 10 48 467 96 493 1.75e-44 162
MS.gene002688.t1 MTR_1g050545 27.126 435 254 10 49 462 85 477 2.51e-44 162
MS.gene002688.t1 MTR_2g095730 28.936 470 259 19 30 467 70 496 1.21e-43 160
MS.gene002688.t1 MTR_1g040370 26.126 444 247 11 40 468 37 414 7.09e-43 157
MS.gene002688.t1 MTR_1g094155 27.700 426 262 14 49 462 92 483 1.35e-41 155
MS.gene002688.t1 MTR_4g057570 26.126 444 258 13 50 467 92 491 4.81e-41 153
MS.gene002688.t1 MTR_5g097180 24.717 441 267 14 48 468 42 437 7.26e-38 144
MS.gene002688.t1 MTR_1g102130 26.411 443 243 16 49 468 107 489 5.34e-37 142
MS.gene002688.t1 MTR_2g018650 55.556 126 36 3 316 432 8 122 1.40e-36 131
MS.gene002688.t1 MTR_3g034290 31.618 272 174 7 33 301 75 337 2.66e-36 139
MS.gene002688.t1 MTR_0003s0560 31.010 287 183 9 33 316 74 348 7.60e-35 133
MS.gene002688.t1 MTR_4g084710 24.434 442 265 14 49 468 45 439 9.45e-31 124
MS.gene002688.t1 MTR_2g095710 25.280 447 278 13 36 468 79 483 6.13e-30 122
MS.gene002688.t1 MTR_2g084530 23.608 449 269 18 48 458 34 446 3.35e-11 65.5
MS.gene002688.t1 MTR_0075s0010 36.957 92 44 3 62 146 35 119 5.67e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002688.t1 AT2G22920 55.204 442 167 5 30 468 22 435 3.46e-175 498
MS.gene002688.t1 AT5G09640 52.903 465 206 8 12 468 6 465 9.10e-175 498
MS.gene002688.t1 AT1G33540 54.875 441 195 4 30 468 26 464 1.02e-174 498
MS.gene002688.t1 AT1G73300 55.730 445 168 4 25 468 25 441 9.06e-174 495
MS.gene002688.t1 AT1G73300 55.730 445 168 4 25 468 25 441 9.06e-174 495
MS.gene002688.t1 AT2G22970 54.545 440 171 4 30 468 22 433 1.61e-173 494
MS.gene002688.t1 AT2G22980 55.856 444 161 6 27 468 20 430 4.86e-173 493
MS.gene002688.t1 AT1G73280 52.665 469 190 5 4 468 1 441 1.06e-172 492
MS.gene002688.t1 AT5G36180 56.180 445 166 4 25 468 25 441 1.40e-172 492
MS.gene002688.t1 AT2G23000 54.773 440 174 4 30 468 22 437 2.17e-170 486
MS.gene002688.t1 AT2G23000 54.773 440 174 4 30 468 22 437 2.17e-170 486
MS.gene002688.t1 AT1G73310 55.056 445 171 4 25 468 25 441 6.52e-170 485
MS.gene002688.t1 AT3G10450 53.034 445 180 4 25 468 21 437 9.37e-170 485
MS.gene002688.t1 AT2G22990 55.000 440 171 5 30 468 20 433 1.21e-169 484
MS.gene002688.t1 AT1G73290 53.483 445 176 5 25 468 24 438 3.51e-168 481
MS.gene002688.t1 AT1G73270 54.318 440 172 3 30 468 30 441 1.81e-167 479
MS.gene002688.t1 AT1G73270 54.318 440 172 3 30 468 30 441 1.85e-167 479
MS.gene002688.t1 AT2G23010 52.070 459 195 4 11 468 3 437 3.13e-166 476
MS.gene002688.t1 AT2G23010 51.634 459 197 4 11 468 3 437 1.32e-164 472
MS.gene002688.t1 AT1G33540 54.524 420 187 4 51 468 4 421 8.09e-164 469
MS.gene002688.t1 AT2G22970 54.348 414 161 3 30 442 22 408 1.32e-163 468
MS.gene002688.t1 AT2G22970 54.327 416 162 3 30 444 22 410 1.38e-163 469
MS.gene002688.t1 AT2G22980 55.502 418 152 5 27 442 20 405 8.17e-162 464
MS.gene002688.t1 AT2G22980 54.273 433 162 6 27 455 20 420 1.59e-161 464
MS.gene002688.t1 AT3G12203 50.218 458 196 4 12 468 11 437 1.90e-161 464
MS.gene002688.t1 AT2G22990 53.409 440 169 6 30 468 20 424 1.37e-160 461
MS.gene002688.t1 AT3G12203 51.136 440 185 3 30 468 6 416 1.93e-160 460
MS.gene002688.t1 AT3G12240 51.236 445 188 4 25 468 20 436 1.63e-158 456
MS.gene002688.t1 AT3G10450 52.506 419 171 3 25 442 21 412 2.26e-157 452
MS.gene002688.t1 AT3G10450 52.029 419 172 4 51 468 4 394 6.58e-157 451
MS.gene002688.t1 AT3G12230 51.016 443 188 4 27 468 21 435 9.71e-157 452
MS.gene002688.t1 AT2G22990 53.023 430 173 5 30 455 20 424 1.00e-156 452
MS.gene002688.t1 AT2G22990 54.106 414 164 4 30 442 20 408 2.35e-156 450
MS.gene002688.t1 AT2G22920 53.616 401 156 4 30 427 22 395 1.57e-153 442
MS.gene002688.t1 AT3G12220 49.887 443 193 3 27 468 21 435 3.77e-152 440
MS.gene002688.t1 AT1G73310 53.676 408 161 3 25 431 25 405 2.35e-151 437
MS.gene002688.t1 AT1G73290 52.723 404 161 4 25 427 24 398 2.87e-148 429
MS.gene002688.t1 AT3G10450 51.399 393 163 3 51 442 4 369 2.12e-144 418
MS.gene002688.t1 AT3G12203 49.265 408 177 3 62 468 1 379 2.08e-140 408
MS.gene002688.t1 AT3G12220 48.687 419 186 3 51 468 15 405 1.28e-138 404
MS.gene002688.t1 AT4G12910 40.496 484 256 7 10 463 11 492 2.56e-127 379
MS.gene002688.t1 AT1G73280 50.129 387 162 4 5 387 2 361 1.06e-126 373
MS.gene002688.t1 AT5G36180 52.589 367 145 4 103 468 1 339 3.68e-126 370
MS.gene002688.t1 AT5G36180 52.589 367 145 4 103 468 1 339 3.68e-126 370
MS.gene002688.t1 AT1G73280 50.954 367 151 4 103 468 1 339 8.63e-125 367
MS.gene002688.t1 AT2G22980 52.055 365 139 6 27 387 20 352 3.95e-122 360
MS.gene002688.t1 AT3G25420 38.144 485 267 7 11 464 8 490 2.12e-121 363
MS.gene002688.t1 AT2G22990 52.174 345 138 5 125 468 1 319 5.17e-118 348
MS.gene002688.t1 AT2G22990 51.594 345 140 5 125 468 1 319 1.42e-116 345
MS.gene002688.t1 AT5G36180 52.047 342 136 3 103 443 1 315 4.51e-116 344
MS.gene002688.t1 AT1G73280 51.170 342 138 4 128 468 1 314 2.96e-115 341
MS.gene002688.t1 AT2G22970 51.097 319 129 2 30 348 22 313 1.33e-113 337
MS.gene002688.t1 AT2G22920 50.938 320 129 3 30 348 22 314 3.41e-112 333
MS.gene002688.t1 AT4G12910 39.597 447 238 7 10 426 11 455 1.02e-108 330
MS.gene002688.t1 AT5G36180 49.425 348 146 4 103 447 1 321 1.91e-105 317
MS.gene002688.t1 AT2G22980 50.938 320 123 4 125 442 1 288 1.50e-104 313
MS.gene002688.t1 AT1G73310 49.240 329 139 3 104 431 2 303 5.90e-104 313
MS.gene002688.t1 AT1G73290 56.705 261 110 2 30 289 29 287 5.50e-103 310
MS.gene002688.t1 AT2G22920 52.500 280 102 5 188 464 1 252 4.74e-95 288
MS.gene002688.t1 AT3G12220 55.745 235 103 1 27 260 21 255 2.32e-83 262
MS.gene002688.t1 AT3G12220 53.793 145 66 1 324 468 240 383 1.55e-48 171
MS.gene002688.t1 AT3G52010 32.280 443 261 12 32 463 64 478 8.09e-67 222
MS.gene002688.t1 AT3G52000 30.942 446 269 10 32 468 63 478 6.30e-66 220
MS.gene002688.t1 AT5G23210 31.489 470 285 16 23 468 38 494 7.14e-65 218
MS.gene002688.t1 AT2G05850 30.684 453 266 16 32 468 63 483 7.10e-63 212
MS.gene002688.t1 AT4G15100 29.835 486 307 14 1 463 1 475 6.51e-62 209
MS.gene002688.t1 AT3G52020 32.373 451 259 13 32 468 79 497 1.11e-61 209
MS.gene002688.t1 AT4G30610 32.308 455 263 17 33 468 33 461 4.15e-61 206
MS.gene002688.t1 AT3G63470 30.254 433 271 10 32 459 81 487 5.22e-59 202
MS.gene002688.t1 AT2G22960 65.441 136 46 1 333 468 50 184 7.54e-59 192
MS.gene002688.t1 AT4G30810 28.166 458 303 12 15 464 18 457 8.99e-59 201
MS.gene002688.t1 AT5G08260 29.688 448 285 14 32 464 39 471 3.44e-57 196
MS.gene002688.t1 AT3G17180 28.474 439 276 13 49 468 55 474 4.60e-57 196
MS.gene002688.t1 AT1G61130 30.493 446 276 15 32 464 31 455 2.61e-56 194
MS.gene002688.t1 AT1G11080 31.696 448 278 14 32 464 50 484 3.96e-56 194
MS.gene002688.t1 AT1G11080 31.696 448 278 14 32 464 50 484 4.68e-56 194
MS.gene002688.t1 AT2G24010 29.730 444 282 12 32 468 30 450 2.85e-55 191
MS.gene002688.t1 AT2G35780 29.888 445 273 15 36 468 33 450 1.38e-54 189
MS.gene002688.t1 AT2G24000 29.596 446 288 12 33 468 31 460 3.54e-54 188
MS.gene002688.t1 AT1G43780 29.867 452 283 14 32 468 37 469 5.79e-54 188
MS.gene002688.t1 AT5G23210 31.221 426 257 15 23 424 38 451 1.28e-53 186
MS.gene002688.t1 AT2G35770 29.712 451 265 12 36 468 41 457 5.72e-53 185
MS.gene002688.t1 AT1G28110 29.438 445 276 13 33 463 32 452 1.63e-52 184
MS.gene002688.t1 AT1G28110 29.438 445 276 13 33 463 32 452 1.63e-52 184
MS.gene002688.t1 AT2G33530 30.000 450 273 16 36 468 37 461 4.46e-52 182
MS.gene002688.t1 AT2G12480 28.731 449 285 13 32 468 31 456 9.31e-52 182
MS.gene002688.t1 AT3G07990 28.160 451 275 13 36 468 37 456 1.54e-50 178
MS.gene002688.t1 AT3G02110 29.663 445 286 14 36 468 41 470 2.22e-50 178
MS.gene002688.t1 AT2G24010 28.540 459 283 13 32 468 30 465 2.85e-50 178
MS.gene002688.t1 AT5G42240 29.295 454 282 15 32 468 32 463 3.95e-50 177
MS.gene002688.t1 AT5G22980 30.093 432 253 15 49 467 100 495 4.83e-50 178
MS.gene002688.t1 AT2G12480 27.679 448 267 12 32 468 31 432 4.12e-49 174
MS.gene002688.t1 AT5G23210 29.950 404 251 14 85 468 8 399 6.90e-49 172
MS.gene002688.t1 AT2G12480 27.455 448 266 12 32 468 31 430 3.02e-48 172
MS.gene002688.t1 AT5G23210 29.777 403 251 14 86 468 8 398 3.51e-48 171
MS.gene002688.t1 AT5G42230 28.667 450 290 15 32 468 28 459 9.83e-47 168
MS.gene002688.t1 AT5G42230 28.667 450 290 15 32 468 28 459 9.83e-47 168
MS.gene002688.t1 AT3G17180 28.141 398 248 13 49 427 55 433 1.07e-46 167
MS.gene002688.t1 AT2G12480 27.394 449 266 13 32 468 31 431 1.35e-46 167
MS.gene002688.t1 AT3G45010 27.563 439 265 12 45 467 99 500 7.21e-46 167
MS.gene002688.t1 AT3G10410 27.739 429 269 11 49 468 101 497 2.24e-45 166
MS.gene002688.t1 AT2G24010 27.066 484 283 14 32 468 1 461 4.15e-45 164
MS.gene002688.t1 AT5G23210 29.248 359 223 13 86 424 8 355 8.01e-37 139
MS.gene002688.t1 AT2G24010 29.462 353 207 11 32 364 1 331 6.61e-33 128
MS.gene002688.t1 AT1G15000 24.186 430 268 15 48 463 36 421 2.54e-31 125
MS.gene002688.t1 AT3G56540 38.217 157 90 3 32 187 79 229 5.28e-30 118
MS.gene002688.t1 AT5G22960 30.303 165 90 2 48 212 35 174 2.46e-25 103
MS.gene002688.t1 AT2G24000 24.828 290 201 7 186 468 118 397 9.33e-20 91.7
MS.gene002688.t1 AT2G27920 23.490 447 272 22 50 458 37 451 3.34e-13 72.0

Find 97 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATTTCCTTATGGGTTTC+TGG -0.005262 2.1:-68292552 MS.gene002688:CDS
TTTGGAACATAAAACATTTC+TGG 0.145142 2.1:+68292943 None:intergenic
ATTGAATGAATTGATATCTT+TGG 0.232350 2.1:+68292925 None:intergenic
ACAAAGTTTAGTTCAACTTA+TGG 0.250544 2.1:-68295261 MS.gene002688:CDS
TGACATGACAATTCCATTCT+TGG 0.262363 2.1:-68292119 MS.gene002688:CDS
GAGTTATTCATATTTCCTTA+TGG 0.267750 2.1:-68292560 MS.gene002688:CDS
TTGTTCGTAGGAAATGAAAA+AGG 0.272446 2.1:-68293529 MS.gene002688:intron
TCTGGTCTTATGCTTGAAAT+TGG 0.286687 2.1:-68294893 MS.gene002688:intron
ATTTCATTTGATTGAAACTT+TGG 0.310987 2.1:+68293719 None:intergenic
AGGTCCACTTGAGTTTAAAA+AGG 0.317116 2.1:-68294218 MS.gene002688:CDS
TGTGGCTTCAAGATCAAATT+AGG 0.326932 2.1:+68294175 None:intergenic
ATATTTCCTTATGGGTTTCT+GGG 0.329687 2.1:-68292551 MS.gene002688:CDS
TCAAATGAAATTTACATTGC+TGG 0.330344 2.1:-68293707 MS.gene002688:CDS
ACATGATAATCATAGCTGTT+AGG 0.338091 2.1:+68292278 None:intergenic
TGATCTTGAAGCCACATTCA+TGG 0.345897 2.1:-68294168 MS.gene002688:CDS
ATAATTTCTTGAACAATTAC+CGG 0.348002 2.1:+68293659 None:intergenic
CAAAGTTTAGTTCAACTTAT+GGG 0.357058 2.1:-68295260 MS.gene002688:CDS
AGGATGATCCTCTCATACTA+TGG 0.360528 2.1:-68294946 MS.gene002688:CDS
AAGCGTTACTCGACAACTTA+AGG 0.360759 2.1:+68292679 None:intergenic
AGTTATTCATATTTCCTTAT+GGG 0.366957 2.1:-68292559 MS.gene002688:CDS
ACTATTCCATTGTTGATGAT+TGG 0.372894 2.1:-68292069 MS.gene002688:CDS
CCTGGTTGCTCTGCTTTGTC+TGG 0.374838 2.1:-68294911 MS.gene002688:CDS
AACATGTCAAAACATTCTTT+AGG 0.382360 2.1:+68291752 None:intergenic
CATACAAATGACCAAGTTGC+AGG 0.394701 2.1:-68292037 MS.gene002688:CDS
TCTATAGTAAAGTTTCTTCC+TGG 0.395127 2.1:-68295174 MS.gene002688:CDS
CCTCTCATACTATGGCTAAC+TGG 0.396512 2.1:-68294938 MS.gene002688:CDS
ATACTCTGGTATTGCTGTTC+CGG 0.407889 2.1:-68293678 MS.gene002688:CDS
TTTCTTCAGGGATACTTGTT+GGG 0.407975 2.1:-68293221 MS.gene002688:intron
TGCAGGGAAGTATTGGAAAA+TGG 0.419352 2.1:-68292340 MS.gene002688:intron
TCTTATGCCACTACAGAATC+TGG 0.426608 2.1:-68293971 MS.gene002688:CDS
CATGATAATCATAGCTGTTA+GGG 0.426927 2.1:+68292279 None:intergenic
AGTCCCATTCCATGAGCAAA+TGG 0.428128 2.1:+68293158 None:intergenic
GCACAAAAGGAAGGGGTCCT+TGG 0.428977 2.1:+68295148 None:intergenic
ATCCCATTTGCTCATGGAAT+GGG 0.431219 2.1:-68293161 MS.gene002688:CDS
ATATTTGTTGTTAAGGTATG+TGG 0.432299 2.1:-68295032 MS.gene002688:intron
GCAATTTGAGATTTGTATTG+AGG 0.443024 2.1:+68292705 None:intergenic
CATGTAAATCTAAGTAGAAA+AGG 0.448093 2.1:-68292260 MS.gene002688:CDS
AGTATGAGAGGATCATCCTT+TGG 0.453644 2.1:+68294950 None:intergenic
CAGGGATACTTGTTGGGCAA+TGG 0.453796 2.1:-68293215 MS.gene002688:CDS
CATGTGTTGCAGGGAAGTAT+TGG 0.455548 2.1:-68292347 MS.gene002688:intron
TTGTTAAGGTATGTGGGAGT+GGG 0.461457 2.1:-68295025 MS.gene002688:intron
TAAGAGAAGCCTGTGGAAAC+CGG 0.462321 2.1:+68293989 None:intergenic
CATGCTTGTGTTGCCAAGAA+TGG 0.464724 2.1:+68292106 None:intergenic
GGAAGGGGTCCTTGGAATCC+AGG 0.464903 2.1:+68295156 None:intergenic
GTTGTTAAGGTATGTGGGAG+TGG 0.467038 2.1:-68295026 MS.gene002688:intron
CATTATATTTGTAGATTCGC+CGG 0.473076 2.1:-68294008 MS.gene002688:CDS
AGTTGCAAATGTCATCTGAT+TGG 0.475334 2.1:+68291896 None:intergenic
GAGATTTGTATTGAGGAACT+TGG 0.479830 2.1:+68292712 None:intergenic
AGCATTATGACGTATATTGT+AGG 0.481619 2.1:+68292307 None:intergenic
AACCCGGTTTCAAGCACAAA+AGG 0.485577 2.1:+68295135 None:intergenic
CATTCTTTAGGTCGGAACTC+TGG 0.487912 2.1:+68291764 None:intergenic
CGAACCATTGATAAATCTCC+AGG 0.488461 2.1:-68293503 MS.gene002688:intron
ATTGCTGGTGATTCATACTC+TGG 0.492272 2.1:-68293692 MS.gene002688:CDS
TGTGGGAGTGGGTGAGAAAG+AGG 0.495771 2.1:-68295014 MS.gene002688:CDS
TATGCAATCCCATTTGCTCA+TGG 0.502070 2.1:-68293167 MS.gene002688:CDS
TATTTGTTGTTAAGGTATGT+GGG 0.503693 2.1:-68295031 MS.gene002688:intron
AAGTTTCTTCCTGGATTCCA+AGG 0.506864 2.1:-68295165 MS.gene002688:CDS
GTAGATTCGCCGGTTTCCAC+AGG 0.511485 2.1:-68293998 MS.gene002688:CDS
TGCTTTCAAGTTGCAACATG+TGG 0.513785 2.1:-68295198 MS.gene002688:CDS
TTAGTAGGTGGATATCTAAG+AGG 0.517206 2.1:-68291730 MS.gene002688:CDS
TGATCAAAATTTCAGGGTGG+AGG 0.517860 2.1:-68291797 MS.gene002688:intron
CTAACCTGGAGATTTATCAA+TGG 0.519088 2.1:+68293499 None:intergenic
AATCCCATTTGCTCATGGAA+TGG 0.520162 2.1:-68293162 MS.gene002688:CDS
TAGTAGGTGGATATCTAAGA+GGG 0.524274 2.1:-68291729 MS.gene002688:CDS
CTATGGCTAACTGGTGGACC+TGG 0.525006 2.1:-68294929 MS.gene002688:CDS
GGTTTCAAGCACAAAAGGAA+GGG 0.526633 2.1:+68295140 None:intergenic
AGCGTTACTCGACAACTTAA+GGG 0.526961 2.1:+68292680 None:intergenic
CATTAGCCCAGAAACCCATA+AGG 0.530053 2.1:+68292545 None:intergenic
CGGTTTCAAGCACAAAAGGA+AGG 0.531662 2.1:+68295139 None:intergenic
CGAACCTTTGTCCATGAATG+TGG 0.535010 2.1:+68294157 None:intergenic
CAATGGTGTAACAACTAGAA+AGG 0.545007 2.1:-68293198 MS.gene002688:CDS
CATTCTTGGCAACACAAGCA+TGG 0.552262 2.1:-68292105 MS.gene002688:CDS
GTCCCATTCCATGAGCAAAT+GGG 0.553573 2.1:+68293159 None:intergenic
GTTTAGTTCAACTTATGGGA+AGG 0.563304 2.1:-68295256 MS.gene002688:CDS
CCAGTTAGCCATAGTATGAG+AGG 0.570952 2.1:+68294938 None:intergenic
GTTTCAAGCACAAAAGGAAG+GGG 0.572128 2.1:+68295141 None:intergenic
TGTCAAAACATTCTTTAGGT+CGG 0.577369 2.1:+68291756 None:intergenic
CCAGACAAAGCAGAGCAACC+AGG 0.587330 2.1:+68294911 None:intergenic
ATTGTTCAAGAAATTATACA+AGG 0.595697 2.1:-68293653 MS.gene002688:intron
TTGTCTCCAATCATCAACAA+TGG 0.609186 2.1:+68292063 None:intergenic
TTCTTAATTAGAGATCTCCT+CGG 0.615291 2.1:+68292743 None:intergenic
CAAAGCACTACACATACGTA+AGG 0.618643 2.1:-68292509 MS.gene002688:intron
GATGACATTTGCAACTGTGA+AGG 0.622581 2.1:-68291889 MS.gene002688:intron
CTCATACTATGGCTAACTGG+TGG 0.626625 2.1:-68294935 MS.gene002688:CDS
GCTTCAAGATCAAATTAGGT+AGG 0.628776 2.1:+68294179 None:intergenic
GAAGCCACATTCATGGACAA+AGG 0.632479 2.1:-68294161 MS.gene002688:intron
ATACAAATGACCAAGTTGCA+GGG 0.634663 2.1:-68292036 MS.gene002688:intron
CTTAGCACCAGATTCTGTAG+TGG 0.636413 2.1:+68293964 None:intergenic
AGATATCAATTCATTCAATG+AGG 0.653803 2.1:-68292921 MS.gene002688:intron
TGAATCAGAGAAGAATCCAA+AGG 0.659518 2.1:-68294966 MS.gene002688:CDS
CATATCTGATGAACTATACG+AGG 0.661127 2.1:-68293135 MS.gene002688:intron
TTGTTGATGATTGGAGACAA+TGG 0.662305 2.1:-68292060 MS.gene002688:CDS
TCGTCACGCCACTCACATAG+CGG 0.692019 2.1:+68292779 None:intergenic
TGTGCAAGTATAGGTACACA+AGG 0.696046 2.1:-68291926 MS.gene002688:intron
GTTACTCGACAACTTAAGGG+TGG 0.723170 2.1:+68292683 None:intergenic
AGTGGCATAAGAGAAGCCTG+TGG 0.736747 2.1:+68293982 None:intergenic
ACGATACTGAAAATTCTCCG+AGG 0.753318 2.1:-68292760 MS.gene002688:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAAAATAGTATAATAAT+TGG - chr2.1:68292419-68292438 MS.gene002688:intron 10.0%
!! TTATTAACAAAAATTTCAAT+TGG + chr2.1:68292169-68292188 None:intergenic 10.0%
!!! GTTTTTTTTTTAATCTATTA+TGG - chr2.1:68291909-68291928 MS.gene002688:CDS 10.0%
!! AAACAAATATTAATTCAACT+TGG + chr2.1:68295033-68295052 None:intergenic 15.0%
!! AAGACATTCAAATTAAAATT+AGG - chr2.1:68292640-68292659 MS.gene002688:intron 15.0%
!! GTTAATAAAGAAAAATGAAT+GGG - chr2.1:68292181-68292200 MS.gene002688:intron 15.0%
!! TAAAAATTTATGGAAAACAA+AGG + chr2.1:68294569-68294588 None:intergenic 15.0%
!! TATAATTTCTTATGGAAAAT+TGG - chr2.1:68294814-68294833 MS.gene002688:intron 15.0%
!! TGTTAATAAAGAAAAATGAA+TGG - chr2.1:68292180-68292199 MS.gene002688:intron 15.0%
!!! TTATTTTTAACACATGATTA+TGG + chr2.1:68295140-68295159 None:intergenic 15.0%
!!! TTTTATATTTACAATAACAG+TGG - chr2.1:68294837-68294856 MS.gene002688:intron 15.0%
!! AATGATTGATCAAAATTTCA+GGG - chr2.1:68295182-68295201 MS.gene002688:CDS 20.0%
!! AGTTATTCATATTTCCTTAT+GGG - chr2.1:68294426-68294445 MS.gene002688:intron 20.0%
!! ATAACATTTCATAATTGATC+TGG + chr2.1:68293744-68293763 None:intergenic 20.0%
!! ATAATTTCTTGAACAATTAC+CGG + chr2.1:68293329-68293348 None:intergenic 20.0%
!! ATTGAATGAATTGATATCTT+TGG + chr2.1:68294063-68294082 None:intergenic 20.0%
!! ATTGTTCAAGAAATTATACA+AGG - chr2.1:68293332-68293351 MS.gene002688:intron 20.0%
!! ATTTCATTTGATTGAAACTT+TGG + chr2.1:68293269-68293288 None:intergenic 20.0%
!! CTATAAATAAATCTTTGTAG+TGG - chr2.1:68293231-68293250 MS.gene002688:intron 20.0%
!! TACTTTAGCTATATAATTCA+TGG - chr2.1:68291878-68291897 MS.gene002688:intron 20.0%
!! TAGATATAATTCATCTTTGT+TGG - chr2.1:68292616-68292635 MS.gene002688:intron 20.0%
!! TATAATTATAATGCAGCAAA+TGG + chr2.1:68294347-68294366 None:intergenic 20.0%
!! TCCTTAAAAAATCATATGTT+TGG + chr2.1:68293070-68293089 None:intergenic 20.0%
!! TGAATGTGGATAAAAATTTA+TGG + chr2.1:68294579-68294598 None:intergenic 20.0%
!!! AACATATGATTTTTTAAGGA+AGG - chr2.1:68293070-68293089 MS.gene002688:intron 20.0%
!!! AATATGAATAACTCTAAAAG+TGG + chr2.1:68294419-68294438 None:intergenic 20.0%
!!! ACCAAACATATGATTTTTTA+AGG - chr2.1:68293066-68293085 MS.gene002688:intron 20.0%
!!! ACTAGTCTTATAATTTCTTA+TGG - chr2.1:68294806-68294825 MS.gene002688:intron 20.0%
!!! ATAGCATAATTTTCGTTATT+TGG + chr2.1:68293215-68293234 None:intergenic 20.0%
!!! ATGAAATGTTATTTTCTTCA+GGG - chr2.1:68293752-68293771 MS.gene002688:intron 20.0%
!!! ATTTATAAGATTTTTTCCCT+TGG - chr2.1:68292943-68292962 MS.gene002688:CDS 20.0%
!!! CATATTCATATTTGTTGTTA+AGG - chr2.1:68291946-68291965 MS.gene002688:intron 20.0%
!!! CATTAAGTTGATCAAAAAAT+CGG + chr2.1:68293630-68293649 None:intergenic 20.0%
!!! TATGAAATGTTATTTTCTTC+AGG - chr2.1:68293751-68293770 MS.gene002688:intron 20.0%
!!! TTAACTTTTGCCAAATATAT+CGG + chr2.1:68292254-68292273 None:intergenic 20.0%
! AAATAAATCTTTGTAGTGGT+TGG - chr2.1:68293235-68293254 MS.gene002688:intron 25.0%
! AACATGTCAAAACATTCTTT+AGG + chr2.1:68295236-68295255 None:intergenic 25.0%
! AACGAAATAATCAATCTTGA+AGG + chr2.1:68293709-68293728 None:intergenic 25.0%
! AATTGAACTGTAAATGTTAC+AGG - chr2.1:68292747-68292766 MS.gene002688:CDS 25.0%
! ACTTGGTCATAAATTTATCA+TGG + chr2.1:68295016-68295035 None:intergenic 25.0%
! AGATATCAATTCATTCAATG+AGG - chr2.1:68294064-68294083 MS.gene002688:intron 25.0%
! CATGTAAATCTAAGTAGAAA+AGG - chr2.1:68294725-68294744 MS.gene002688:intron 25.0%
! GAATGATTGATCAAAATTTC+AGG - chr2.1:68295181-68295200 MS.gene002688:CDS 25.0%
! GAGTTATTCATATTTCCTTA+TGG - chr2.1:68294425-68294444 MS.gene002688:intron 25.0%
! GATATAAGTATAGCTATGTA+TGG + chr2.1:68293921-68293940 None:intergenic 25.0%
! TCAAATGAAATTTACATTGC+TGG - chr2.1:68293278-68293297 MS.gene002688:intron 25.0%
! TGTAAAGAAATTTACTGATG+TGG - chr2.1:68292704-68292723 MS.gene002688:CDS 25.0%
! TTAAAAAGGAGGAATACAAT+GGG - chr2.1:68292781-68292800 MS.gene002688:CDS 25.0%
! TTGTTAGTACTTATGTCAAT+CGG - chr2.1:68293185-68293204 MS.gene002688:CDS 25.0%
! TTTAAAAAGGAGGAATACAA+TGG - chr2.1:68292780-68292799 MS.gene002688:CDS 25.0%
!! ACAAAGTTTAGTTCAACTTA+TGG - chr2.1:68291724-68291743 MS.gene002688:CDS 25.0%
!! CAAAGTTTAGTTCAACTTAT+GGG - chr2.1:68291725-68291744 MS.gene002688:CDS 25.0%
!! CTTTTCAAATTGTTCACTTT+TGG - chr2.1:68292128-68292147 MS.gene002688:CDS 25.0%
!! TTTGGAACATAAAACATTTC+TGG + chr2.1:68294045-68294064 None:intergenic 25.0%
!!! AATGTTTTGACATGTTTAGT+AGG - chr2.1:68295240-68295259 MS.gene002688:CDS 25.0%
!!! ATATTTGTTGTTAAGGTATG+TGG - chr2.1:68291953-68291972 MS.gene002688:intron 25.0%
!!! TATTTGTTGTTAAGGTATGT+GGG - chr2.1:68291954-68291973 MS.gene002688:intron 25.0%
AAATCAGGTGACAAAAAATG+TGG + chr2.1:68292863-68292882 None:intergenic 30.0%
ACATGATAATCATAGCTGTT+AGG + chr2.1:68294710-68294729 None:intergenic 30.0%
AGCATTATGACGTATATTGT+AGG + chr2.1:68294681-68294700 None:intergenic 30.0%
ATAAGTATAGCTATGTATGG+AGG + chr2.1:68293918-68293937 None:intergenic 30.0%
ATATTTCCTTATGGGTTTCT+GGG - chr2.1:68294434-68294453 MS.gene002688:intron 30.0%
CAATTAGTTAGCGTATATCT+CGG + chr2.1:68293961-68293980 None:intergenic 30.0%
CATGATAATCATAGCTGTTA+GGG + chr2.1:68294709-68294728 None:intergenic 30.0%
CATTATATTTGTAGATTCGC+CGG - chr2.1:68292977-68292996 MS.gene002688:CDS 30.0%
TAGCTAAAGTAAAACTAACC+CGG + chr2.1:68291869-68291888 None:intergenic 30.0%
TATACAAGGTACAAAACTCA+TGG - chr2.1:68293346-68293365 MS.gene002688:intron 30.0%
TCGCTGCAAAAAAATTGTAA+TGG - chr2.1:68293999-68294018 MS.gene002688:CDS 30.0%
TCTATAGTAAAGTTTCTTCC+TGG - chr2.1:68291811-68291830 MS.gene002688:intron 30.0%
TGATACTTTAAATGCTAACC+TGG + chr2.1:68293503-68293522 None:intergenic 30.0%
TGCAATCAAGTTCTTATGTA+AGG + chr2.1:68294620-68294639 None:intergenic 30.0%
TGTCAAAACATTCTTTAGGT+CGG + chr2.1:68295232-68295251 None:intergenic 30.0%
TGTTCGTAGGAAATGAAAAA+GGG - chr2.1:68293457-68293476 MS.gene002688:intron 30.0%
TTACAAATTCACTTGTTCGT+AGG - chr2.1:68293444-68293463 MS.gene002688:intron 30.0%
TTCTTAATTAGAGATCTCCT+CGG + chr2.1:68294245-68294264 None:intergenic 30.0%
TTGTTCGTAGGAAATGAAAA+AGG - chr2.1:68293456-68293475 MS.gene002688:intron 30.0%
! AGAGTTGTCACATATTCTTT+TGG - chr2.1:68292509-68292528 MS.gene002688:intron 30.0%
! AGTTCATCAGTGTTTTCTTA+TGG + chr2.1:68294789-68294808 None:intergenic 30.0%
! GAATAACTCTAAAAGTGGAA+AGG + chr2.1:68294414-68294433 None:intergenic 30.0%
! GCAATTTGAGATTTGTATTG+AGG + chr2.1:68294283-68294302 None:intergenic 30.0%
!! ACTATTCCATTGTTGATGAT+TGG - chr2.1:68294916-68294935 MS.gene002688:CDS 30.0%
!! GAATGCAAAAGTTCTAAATC+AGG + chr2.1:68292878-68292897 None:intergenic 30.0%
!! TTTTAACTGTGTGCAAGTAT+AGG - chr2.1:68295050-68295069 MS.gene002688:intron 30.0%
!!! ATAAGATTTTTTCCCTTGGT+AGG - chr2.1:68292947-68292966 MS.gene002688:CDS 30.0%
AAATCTTCACCAACCATAAG+GGG + chr2.1:68292595-68292614 None:intergenic 35.0%
AAGTATCACACTCTCATGTT+AGG - chr2.1:68293515-68293534 MS.gene002688:CDS 35.0%
AGAAATCTTCACCAACCATA+AGG + chr2.1:68292597-68292616 None:intergenic 35.0%
AGGTCCACTTGAGTTTAAAA+AGG - chr2.1:68292767-68292786 MS.gene002688:CDS 35.0%
AGTTGCAAATGTCATCTGAT+TGG + chr2.1:68295092-68295111 None:intergenic 35.0%
ATAATGTTACTCACCTACCA+AGG + chr2.1:68292963-68292982 None:intergenic 35.0%
ATACAAATGACCAAGTTGCA+GGG - chr2.1:68294949-68294968 MS.gene002688:CDS 35.0%
ATATCGAATTGACACATGGA+TGG + chr2.1:68292228-68292247 None:intergenic 35.0%
ATTATGGCCCTCATTGTTTA+AGG + chr2.1:68295124-68295143 None:intergenic 35.0%
CAATGGTGTAACAACTAGAA+AGG - chr2.1:68293787-68293806 MS.gene002688:intron 35.0%
CATATCTGATGAACTATACG+AGG - chr2.1:68293850-68293869 MS.gene002688:intron 35.0%
CATATTTCCTTATGGGTTTC+TGG - chr2.1:68294433-68294452 MS.gene002688:intron 35.0%
CTAACCTGGAGATTTATCAA+TGG + chr2.1:68293489-68293508 None:intergenic 35.0%
GAAATCTTCACCAACCATAA+GGG + chr2.1:68292596-68292615 None:intergenic 35.0%
GAGATTTGTATTGAGGAACT+TGG + chr2.1:68294276-68294295 None:intergenic 35.0%
GATTGATCAAAATTTCAGGG+TGG - chr2.1:68295185-68295204 MS.gene002688:CDS 35.0%
GCTTCAAGATCAAATTAGGT+AGG + chr2.1:68292809-68292828 None:intergenic 35.0%
TAATGTTACTCACCTACCAA+GGG + chr2.1:68292962-68292981 None:intergenic 35.0%
TAGTAGGTGGATATCTAAGA+GGG - chr2.1:68295256-68295275 MS.gene002688:CDS 35.0%
TCCACTTGAGTTTAAAAAGG+AGG - chr2.1:68292770-68292789 MS.gene002688:CDS 35.0%
TGAATCAGAGAAGAATCCAA+AGG - chr2.1:68292019-68292038 MS.gene002688:intron 35.0%
TGACATGACAATTCCATTCT+TGG - chr2.1:68294866-68294885 MS.gene002688:intron 35.0%
TGTGGCTTCAAGATCAAATT+AGG + chr2.1:68292813-68292832 None:intergenic 35.0%
TTAGTAGGTGGATATCTAAG+AGG - chr2.1:68295255-68295274 MS.gene002688:CDS 35.0%
TTGTCTCCAATCATCAACAA+TGG + chr2.1:68294925-68294944 None:intergenic 35.0%
TTTCTTCAGGGATACTTGTT+GGG - chr2.1:68293764-68293783 MS.gene002688:intron 35.0%
! ATTTTGAATACCCTGCAACT+TGG + chr2.1:68294962-68294981 None:intergenic 35.0%
! GTTTAGTTCAACTTATGGGA+AGG - chr2.1:68291729-68291748 MS.gene002688:CDS 35.0%
! TATTTTTCACAGCTCACATC+AGG - chr2.1:68294158-68294177 MS.gene002688:intron 35.0%
! TCCTCCTTTTTAAACTCAAG+TGG + chr2.1:68292774-68292793 None:intergenic 35.0%
! TCGATATGCACCGATATATT+TGG - chr2.1:68292241-68292260 MS.gene002688:CDS 35.0%
! TTTTCTTCAGGGATACTTGT+TGG - chr2.1:68293763-68293782 MS.gene002688:intron 35.0%
!! GAAGGTACCTTAAACAATGA+GGG - chr2.1:68295114-68295133 MS.gene002688:intron 35.0%
!! GTTTTGACATGTTTAGTAGG+TGG - chr2.1:68295243-68295262 MS.gene002688:CDS 35.0%
!! TCTGGTCTTATGCTTGAAAT+TGG - chr2.1:68292092-68292111 MS.gene002688:CDS 35.0%
!! TGAAGGTACCTTAAACAATG+AGG - chr2.1:68295113-68295132 MS.gene002688:intron 35.0%
!! TTGTTGATGATTGGAGACAA+TGG - chr2.1:68294925-68294944 MS.gene002688:CDS 35.0%
AACTTGATTGCATGTGTTGC+AGG - chr2.1:68294628-68294647 MS.gene002688:intron 40.0%
AAGCGTTACTCGACAACTTA+AGG + chr2.1:68294309-68294328 None:intergenic 40.0%
AAGTTTCTTCCTGGATTCCA+AGG - chr2.1:68291820-68291839 MS.gene002688:intron 40.0%
AATCCCATTTGCTCATGGAA+TGG - chr2.1:68293823-68293842 MS.gene002688:intron 40.0%
ACATTCATGGACAAAGGTTC+GGG - chr2.1:68292830-68292849 MS.gene002688:intron 40.0%
ACGATACTGAAAATTCTCCG+AGG - chr2.1:68294225-68294244 MS.gene002688:intron 40.0%
ACTTGATTGCATGTGTTGCA+GGG - chr2.1:68294629-68294648 MS.gene002688:intron 40.0%
AGCGTTACTCGACAACTTAA+GGG + chr2.1:68294308-68294327 None:intergenic 40.0%
AGGATGATCCTCTCATACTA+TGG - chr2.1:68292039-68292058 MS.gene002688:CDS 40.0%
AGTATGAGAGGATCATCCTT+TGG + chr2.1:68292038-68292057 None:intergenic 40.0%
ATCCCATTTGCTCATGGAAT+GGG - chr2.1:68293824-68293843 MS.gene002688:intron 40.0%
ATTGCTGGTGATTCATACTC+TGG - chr2.1:68293293-68293312 MS.gene002688:intron 40.0%
CACATTCATGGACAAAGGTT+CGG - chr2.1:68292829-68292848 MS.gene002688:intron 40.0%
CATACAAATGACCAAGTTGC+AGG - chr2.1:68294948-68294967 MS.gene002688:CDS 40.0%
CGAACCATTGATAAATCTCC+AGG - chr2.1:68293482-68293501 MS.gene002688:intron 40.0%
GATGACATTTGCAACTGTGA+AGG - chr2.1:68295096-68295115 MS.gene002688:intron 40.0%
GGTTTCAAGCACAAAAGGAA+GGG + chr2.1:68291848-68291867 None:intergenic 40.0%
GTGCATATCGAATTGACACA+TGG + chr2.1:68292232-68292251 None:intergenic 40.0%
GTTTCAAGCACAAAAGGAAG+GGG + chr2.1:68291847-68291866 None:intergenic 40.0%
TATGCAATCCCATTTGCTCA+TGG - chr2.1:68293818-68293837 MS.gene002688:intron 40.0%
TCTTATGCCACTACAGAATC+TGG - chr2.1:68293014-68293033 MS.gene002688:intron 40.0%
TGATCAAAATTTCAGGGTGG+AGG - chr2.1:68295188-68295207 MS.gene002688:CDS 40.0%
TGATCTTGAAGCCACATTCA+TGG - chr2.1:68292817-68292836 MS.gene002688:CDS 40.0%
TGTGCAAGTATAGGTACACA+AGG - chr2.1:68295059-68295078 MS.gene002688:intron 40.0%
TTGTTAAGGTATGTGGGAGT+GGG - chr2.1:68291960-68291979 MS.gene002688:intron 40.0%
! ATACTCTGGTATTGCTGTTC+CGG - chr2.1:68293307-68293326 MS.gene002688:intron 40.0%
! CAAAGCACTACACATACGTA+AGG - chr2.1:68294476-68294495 MS.gene002688:intron 40.0%
! CTTCCTTTTGTGCTTGAAAC+CGG - chr2.1:68291847-68291866 MS.gene002688:intron 40.0%
! TTCCTTTTGTGCTTGAAACC+GGG - chr2.1:68291848-68291867 MS.gene002688:intron 40.0%
! TTTTAGATCCGCTATGTGAG+TGG - chr2.1:68294198-68294217 MS.gene002688:CDS 40.0%
!! TGCAGGGAAGTATTGGAAAA+TGG - chr2.1:68294645-68294664 MS.gene002688:intron 40.0%
!! TGCTTTCAAGTTGCAACATG+TGG - chr2.1:68291787-68291806 MS.gene002688:intron 40.0%
AACCCGGTTTCAAGCACAAA+AGG + chr2.1:68291853-68291872 None:intergenic 45.0%
AGTCCCATTCCATGAGCAAA+TGG + chr2.1:68293830-68293849 None:intergenic 45.0%
CATGCTTGTGTTGCCAAGAA+TGG + chr2.1:68294882-68294901 None:intergenic 45.0%
CATTAGCCCAGAAACCCATA+AGG + chr2.1:68294443-68294462 None:intergenic 45.0%
CATTCTTGGCAACACAAGCA+TGG - chr2.1:68294880-68294899 MS.gene002688:intron 45.0%
CATTCTTTAGGTCGGAACTC+TGG + chr2.1:68295224-68295243 None:intergenic 45.0%
CCAACCATAAGGGGTTAACA+AGG + chr2.1:68292586-68292605 None:intergenic 45.0%
CCAGTTAGCCATAGTATGAG+AGG + chr2.1:68292050-68292069 None:intergenic 45.0%
CCTCTCATACTATGGCTAAC+TGG - chr2.1:68292047-68292066 MS.gene002688:CDS 45.0%
CCTTGTTAACCCCTTATGGT+TGG - chr2.1:68292583-68292602 MS.gene002688:intron 45.0%
CGAACCTTTGTCCATGAATG+TGG + chr2.1:68292831-68292850 None:intergenic 45.0%
CGGTTTCAAGCACAAAAGGA+AGG + chr2.1:68291849-68291868 None:intergenic 45.0%
CTCATACTATGGCTAACTGG+TGG - chr2.1:68292050-68292069 MS.gene002688:CDS 45.0%
CTTAGCACCAGATTCTGTAG+TGG + chr2.1:68293024-68293043 None:intergenic 45.0%
GAAGCCACATTCATGGACAA+AGG - chr2.1:68292824-68292843 MS.gene002688:intron 45.0%
GTCCCATTCCATGAGCAAAT+GGG + chr2.1:68293829-68293848 None:intergenic 45.0%
GTTACTCGACAACTTAAGGG+TGG + chr2.1:68294305-68294324 None:intergenic 45.0%
GTTGTTAAGGTATGTGGGAG+TGG - chr2.1:68291959-68291978 MS.gene002688:intron 45.0%
TAAGAGAAGCCTGTGGAAAC+CGG + chr2.1:68292999-68293018 None:intergenic 45.0%
! CAAAAAATCGGTGCTTGCGT+CGG + chr2.1:68293618-68293637 None:intergenic 45.0%
! CATGTGTTGCAGGGAAGTAT+TGG - chr2.1:68294638-68294657 MS.gene002688:intron 45.0%
!! AACAAGGCTAGCCTACATGA+GGG + chr2.1:68292570-68292589 None:intergenic 45.0%
!! CGTATATCTCGGTGCTTCAT+AGG + chr2.1:68293950-68293969 None:intergenic 45.0%
!! CTAGCCTTGTTAACCCCTTA+TGG - chr2.1:68292579-68292598 MS.gene002688:intron 45.0%
!! TAACAAGGCTAGCCTACATG+AGG + chr2.1:68292571-68292590 None:intergenic 45.0%
AGTGGCATAAGAGAAGCCTG+TGG + chr2.1:68293006-68293025 None:intergenic 50.0%
CTCAACTTCCTCCCTCATGT+AGG - chr2.1:68292556-68292575 MS.gene002688:CDS 50.0%
! CAGGGATACTTGTTGGGCAA+TGG - chr2.1:68293770-68293789 MS.gene002688:intron 50.0%
CCAGACAAAGCAGAGCAACC+AGG + chr2.1:68292077-68292096 None:intergenic 55.0%
CCTGGTTGCTCTGCTTTGTC+TGG - chr2.1:68292074-68292093 MS.gene002688:CDS 55.0%
GCACAAAAGGAAGGGGTCCT+TGG + chr2.1:68291840-68291859 None:intergenic 55.0%
TCGTCACGCCACTCACATAG+CGG + chr2.1:68294209-68294228 None:intergenic 55.0%
TGTGCGACGTCGCATGAATG+TGG + chr2.1:68294593-68294612 None:intergenic 55.0%
TGTGGGAGTGGGTGAGAAAG+AGG - chr2.1:68291971-68291990 MS.gene002688:intron 55.0%
! CTATGGCTAACTGGTGGACC+TGG - chr2.1:68292056-68292075 MS.gene002688:CDS 55.0%
! TGCTTGCGTCGGTGCTTTCT+AGG + chr2.1:68293607-68293626 None:intergenic 55.0%
!! AAGGCTAGCCTACATGAGGG+AGG + chr2.1:68292567-68292586 None:intergenic 55.0%
!! GTAGATTCGCCGGTTTCCAC+AGG - chr2.1:68292987-68293006 MS.gene002688:CDS 55.0%
GGAAGGGGTCCTTGGAATCC+AGG + chr2.1:68291832-68291851 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 68291721 68295286 68291721 ID=MS.gene002688
chr2.1 mRNA 68291721 68295286 68291721 ID=MS.gene002688.t1;Parent=MS.gene002688
chr2.1 exon 68295138 68295286 68295138 ID=MS.gene002688.t1.exon1;Parent=MS.gene002688.t1
chr2.1 CDS 68295138 68295286 68295138 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
chr2.1 exon 68294894 68295039 68294894 ID=MS.gene002688.t1.exon2;Parent=MS.gene002688.t1
chr2.1 CDS 68294894 68295039 68294894 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
chr2.1 exon 68294162 68294238 68294162 ID=MS.gene002688.t1.exon3;Parent=MS.gene002688.t1
chr2.1 CDS 68294162 68294238 68294162 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
chr2.1 exon 68293916 68294038 68293916 ID=MS.gene002688.t1.exon4;Parent=MS.gene002688.t1
chr2.1 CDS 68293916 68294038 68293916 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
chr2.1 exon 68293654 68293756 68293654 ID=MS.gene002688.t1.exon5;Parent=MS.gene002688.t1
chr2.1 CDS 68293654 68293756 68293654 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
chr2.1 exon 68293504 68293541 68293504 ID=MS.gene002688.t1.exon6;Parent=MS.gene002688.t1
chr2.1 CDS 68293504 68293541 68293504 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
chr2.1 exon 68293136 68293234 68293136 ID=MS.gene002688.t1.exon7;Parent=MS.gene002688.t1
chr2.1 CDS 68293136 68293234 68293136 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
chr2.1 exon 68292922 68293008 68292922 ID=MS.gene002688.t1.exon8;Parent=MS.gene002688.t1
chr2.1 CDS 68292922 68293008 68292922 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
chr2.1 exon 68292688 68292837 68292688 ID=MS.gene002688.t1.exon9;Parent=MS.gene002688.t1
chr2.1 CDS 68292688 68292837 68292688 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
chr2.1 exon 68292510 68292581 68292510 ID=MS.gene002688.t1.exon10;Parent=MS.gene002688.t1
chr2.1 CDS 68292510 68292581 68292510 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
chr2.1 exon 68292239 68292357 68292239 ID=MS.gene002688.t1.exon11;Parent=MS.gene002688.t1
chr2.1 CDS 68292239 68292357 68292239 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
chr2.1 exon 68292037 68292150 68292037 ID=MS.gene002688.t1.exon12;Parent=MS.gene002688.t1
chr2.1 CDS 68292037 68292150 68292037 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
chr2.1 exon 68291890 68291935 68291890 ID=MS.gene002688.t1.exon13;Parent=MS.gene002688.t1
chr2.1 CDS 68291890 68291935 68291890 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
chr2.1 exon 68291721 68291804 68291721 ID=MS.gene002688.t1.exon14;Parent=MS.gene002688.t1
chr2.1 CDS 68291721 68291804 68291721 ID=cds.MS.gene002688.t1;Parent=MS.gene002688.t1
Gene Sequence

>MS.gene002688

ATGACAAAGTTTAGTTCAACTTATGGGAAGGTTCTTCTTTCTGTTTTTCTTTTATTATCACATATTTGCTTTCAAGTTGCAACATGTGGCTCTATAGTAAAGTTTCTTCCTGGATTCCAAGGACCCCTTCCTTTTGTGCTTGAAACCGGGTTAGTTTTACTTTAGCTATATAATTCATGGCTTTACAAGTTTTTTTTTTAATCTATTATGGTATTTAAATAATTTCATATTCATATTTGTTGTTAAGGTATGTGGGAGTGGGTGAGAAAGAGGATGTGCAAGTGTTTTACTATTTCATTGAATCAGAGAAGAATCCAAAGGATGATCCTCTCATACTATGGCTAACTGGTGGACCTGGTTGCTCTGCTTTGTCTGGTCTTATGCTTGAAATTGGTAATTGCAACTTGCTTTTCAAATTGTTCACTTTTGGATAGTCTTACAATAACCAATTGAAATTTTTGTTAATAAAGAAAAATGAATGGGTAAATGTTAGTGAGAATTTTTCCATCCATGTGTCAATTCGATATGCACCGATATATTTGGCAAAAGTTAATTTTTGAAAAAAAAAATTATTTTCTATCATATAAAAAGTTATATTAATTAAATTATATTTATCAAAGTGTATGCGAAATAAATTTAATTTTTTTCAATTTGTTTTCTCGTAATAGTTAATATTGAATTATTTTACGATAAAATGTCAAAAAAATAGTATAATAATTGGAAATAATGAAATGATTTTTTTCTAATGAAATATGTGACAACTCTATGATTTTTTACTAAAAATTTATAGAGTTGTCACATATTCTTTTGGTTTATATTTAATATTTCGCATTCTCTCAACTTCCTCCCTCATGTAGGCTAGCCTTGTTAACCCCTTATGGTTGGTGAAGATTTCTAGATATAATTCATCTTTGTTGGAAAGACATTCAAATTAAAATTAGGAAGATCAATAGACACTGCAATGATACTAATTGAAGTGTCAATGTAAAGAAATTTACTGATGTGGTAAAATGATACTCATTGAATAATTGAACTGTAAATGTTACAGGTCCACTTGAGTTTAAAAAGGAGGAATACAATGGGAGCCTACCTAATTTGATCTTGAAGCCACATTCATGGACAAAGGTTCGGGTCATAGTCCACATTTTTTGTCACCTGATTTAGAACTTTTGCATTCAATGTATTTATCAAATTCTTTTAAATTATATATTTATTTACTAGTATTTATAAGATTTTTTCCCTTGGTAGGTGAGTAACATTATATTTGTAGATTCGCCGGTTTCCACAGGCTTCTCTTATGCCACTACAGAATCTGGTGCTAAGCGAAGCGACTCGATTCTAGTTCACCAAACATATGATTTTTTAAGGAAGGTATACATTTCACAATAATCACTTTTTTTATGTTTTAAAACTTTGTTTTTTTTTTTTTATTTTATTTTAAAAAGAAGTGTGTTTTTTTTAATTTTGTTAGTACTTATGTCAATCGGTCAACCAAATAACGAAAATTATGCTATAAATAAATCTTTGTAGTGGTTGGTTGATCATCCAAAGTTTCAATCAAATGAAATTTACATTGCTGGTGATTCATACTCTGGTATTGCTGTTCCGGTAATTGTTCAAGAAATTATACAAGGTACAAAACTCATGGCTCAATTATGTATTGCAGAGCGATTGTGTTACAATTGATTTACTGTATGTTGTAATTTTAATTAAGTTTAATTATTTACAAATTCACTTGTTCGTAGGAAATGAAAAAGGGATCGAACCATTGATAAATCTCCAGGTTAGCATTTAAAGTATCACACTCTCATGTTAGGAATGCATTTTTTCAACTTACGTTGTATGTTTCAATAATGTGAAAATTGCATTTCAACTACTGAAAACCTAGAAAGCACCGACGCAAGCACCGATTTTTTGATCAACTTAATGTTTCGATCAAATAGATTATGCATGACGTGTAATAAAACAATCGACTACATACAATACCTTCAAGATTGATTATTTCGTTATATAACAGCTTCCAGATCAATTATGAAATGTTATTTTCTTCAGGGATACTTGTTGGGCAATGGTGTAACAACTAGAAAGGAAAAAAATTATGCAATCCCATTTGCTCATGGAATGGGACTCATATCTGATGAACTATACGAGGTAACTGAATTATACTTTCACAGTATCTAATTGAGTTATAATTCCTCCATACATAGCTATACTTATATCGAAAAACCTATGAAGCACCGAGATATACGCTAACTAATTGATATCTGCGATTTTGTAGTCGCTGCAAAAAAATTGTAATGGAGACTATGTAAATGCAGAGACCAGAAATGTTTTATGTTCCAAAGATATCAATTCATTCAATGAGGTATATGTTTATTTTATTTTTGTCAAATTTCTCAAGTAGAATTACTAGTCACAAAAATGAACATCATTCTTCTATTTTTCACAGCTCACATCAGGACTTAATACAGCACATATTTTAGATCCGCTATGTGAGTGGCGTGACGATACTGAAAATTCTCCGAGGAGATCTCTAATTAAGAATTATTTCTCCAAGTTCCTCAATACAAATCTCAAATTGCCACCCTTAAGTTGTCGAGTAACGCTTCTTACTATATATATACCATTTGCTGCATTATAATTATAAATATATATAAAGCTCAATTTTTTTGTTAACTTGATGTTTGCTTCCTTTCCACTTTTAGAGTTATTCATATTTCCTTATGGGTTTCTGGGCTAATGATGATAATGTTCGCAAAGCACTACACATACGTAAGGTATATTATTGATACATATATGCGAAAGTTGTAATTTCTTCATGCTATCTGTATATTCATATTTCACACCTTTGTTTTCCATAAATTTTTATCCACATTCATGCGACGTCGCACATAGTCCTTACATAAGAACTTGATTGCATGTGTTGCAGGGAAGTATTGGAAAATGGCATCGTTGCACCTACAATATACGTCATAATGCTGATATCCCTAACAGCTATGATTATCATGTAAATCTAAGTAGAAAAGGCATTCGTTCACTAATTTACAAGTCAGTAATCTTTCTCTCCATAAGAAAACACTGATGAACTAGTCTTATAATTTCTTATGGAAAATTGGTTTTATATTTACAATAACAGTGGCGATCATGACATGACAATTCCATTCTTGGCAACACAAGCATGGATAAGATCTTTGAACTATTCCATTGTTGATGATTGGAGACAATGGCATACAAATGACCAAGTTGCAGGGTATTCAAAATTTCTAATTGTTATAGTCATTATATATAATAACCATGATAAATTTATGACCAAGTTGAATTAATATTTGTTTTAACTGTGTGCAAGTATAGGTACACAAGGACTTACTCCAATCAGATGACATTTGCAACTGTGAAGGTACCTTAAACAATGAGGGCCATAATCATGTGTTAAAAATAATGACTAGTTTATTTACTCTATGAATGATTGATCAAAATTTCAGGGTGGAGGACACACAGCTCCAGAGTTCCGACCTAAAGAATGTTTTGACATGTTTAGTAGGTGGATATCTAAGAGGGTTTTGTAG
Protein sequence

>MS.gene002688.t1

MTKFSSTYGKVLLSVFLLLSHICFQVATCGSIVKFLPGFQGPLPFVLETGYVGVGEKEDVQVFYYFIESEKNPKDDPLILWLTGGPGCSALSGLMLEIGPLEFKKEEYNGSLPNLILKPHSWTKVSNIIFVDSPVSTGFSYATTESGAKRSDSILVHQTYDFLRKWLVDHPKFQSNEIYIAGDSYSGIAVPVIVQEIIQGNEKGIEPLINLQGYLLGNGVTTRKEKNYAIPFAHGMGLISDELYESLQKNCNGDYVNAETRNVLCSKDINSFNELTSGLNTAHILDPLCEWRDDTENSPRRSLIKNYFSKFLNTNLKLPPLSCRSYSYFLMGFWANDDNVRKALHIRKGSIGKWHRCTYNIRHNADIPNSYDYHVNLSRKGIRSLIYNGDHDMTIPFLATQAWIRSLNYSIVDDWRQWHTNDQVAGYTRTYSNQMTFATVKGGGHTAPEFRPKECFDMFSRWISKRVL