AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004369

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004369.t1 MTR_2g018600 85.075 469 69 1 1 468 1 469 0.0 818
MS.gene004369.t1 MTR_2g018590 83.369 469 77 1 1 468 1 469 0.0 816
MS.gene004369.t1 MTR_2g018630 72.889 450 113 3 20 468 24 465 0.0 681
MS.gene004369.t1 MTR_3g100420 64.668 467 155 4 12 468 30 496 0.0 618
MS.gene004369.t1 MTR_4g021520 63.474 449 157 5 21 468 28 470 0.0 572
MS.gene004369.t1 MTR_4g021510 61.171 461 171 6 10 468 15 469 0.0 560
MS.gene004369.t1 MTR_4g021500 61.174 443 165 5 21 462 28 464 0.0 550
MS.gene004369.t1 MTR_4g021500 58.499 453 179 6 18 468 460 905 4.15e-180 528
MS.gene004369.t1 MTR_4g021500 61.174 443 165 5 21 462 28 464 0.0 549
MS.gene004369.t1 MTR_4g021500 57.174 453 167 7 18 468 460 887 1.70e-170 502
MS.gene004369.t1 MTR_4g021490 59.911 449 173 5 21 468 28 470 0.0 547
MS.gene004369.t1 MTR_2g018630 71.390 367 96 3 103 468 2 360 0.0 544
MS.gene004369.t1 MTR_4g021530 59.259 459 176 6 15 468 18 470 0.0 540
MS.gene004369.t1 MTR_4g021535 57.906 468 179 7 7 468 11 466 0.0 538
MS.gene004369.t1 MTR_4g021520 62.408 407 146 5 21 426 28 428 1.50e-177 504
MS.gene004369.t1 MTR_4g021535 50.855 468 165 8 7 468 11 419 2.17e-154 445
MS.gene004369.t1 MTR_3g045770 42.576 458 241 5 12 464 10 450 4.71e-132 389
MS.gene004369.t1 MTR_8g011350 38.821 492 259 9 10 464 5 491 4.46e-119 358
MS.gene004369.t1 MTR_7g080180 38.956 498 251 10 13 468 12 498 2.48e-118 356
MS.gene004369.t1 MTR_2g009200 39.956 458 234 7 12 463 12 434 1.09e-115 347
MS.gene004369.t1 MTR_7g051430 37.755 490 261 7 12 464 9 491 3.06e-115 348
MS.gene004369.t1 MTR_8g011350 38.380 469 247 9 10 441 5 468 1.58e-107 327
MS.gene004369.t1 MTR_7g080180 37.939 456 230 10 13 426 12 456 3.24e-98 303
MS.gene004369.t1 MTR_7g051430 34.383 413 227 7 12 387 9 414 8.75e-77 246
MS.gene004369.t1 MTR_4g075150 31.448 442 281 13 33 468 35 460 1.02e-61 208
MS.gene004369.t1 MTR_4g075150 31.685 445 278 14 33 468 35 462 1.60e-61 207
MS.gene004369.t1 MTR_2g063000 28.859 447 288 12 31 468 37 462 4.67e-58 199
MS.gene004369.t1 MTR_8g099930 30.952 462 277 15 24 468 25 461 1.04e-56 195
MS.gene004369.t1 MTR_3g434850 30.155 451 272 16 33 468 31 453 3.64e-56 193
MS.gene004369.t1 MTR_7g013790 29.449 472 295 14 12 468 15 463 4.84e-56 193
MS.gene004369.t1 MTR_3g498755 30.803 461 263 15 33 468 15 444 1.11e-55 192
MS.gene004369.t1 MTR_2g012860 29.935 461 298 14 18 468 27 472 1.26e-55 192
MS.gene004369.t1 MTR_2g062990 28.184 479 311 14 2 468 4 461 4.28e-55 191
MS.gene004369.t1 MTR_2g062980 28.184 479 311 14 2 468 4 461 7.20e-55 190
MS.gene004369.t1 MTR_2g081620 29.252 441 284 13 35 468 43 462 7.75e-55 190
MS.gene004369.t1 MTR_4g122960 29.053 475 302 17 12 464 9 470 3.06e-54 189
MS.gene004369.t1 MTR_1g095560 29.140 477 298 16 9 468 2 455 7.19e-54 187
MS.gene004369.t1 MTR_1g095540 28.330 473 301 14 12 468 5 455 1.34e-53 186
MS.gene004369.t1 MTR_4g122950 28.392 479 302 18 12 464 9 472 1.62e-53 187
MS.gene004369.t1 MTR_2g012850 30.474 443 279 16 32 463 35 459 5.94e-53 185
MS.gene004369.t1 MTR_3g079590 30.435 437 268 12 33 463 78 484 3.86e-52 183
MS.gene004369.t1 MTR_5g072570 28.541 473 301 15 12 468 9 460 6.44e-52 182
MS.gene004369.t1 MTR_3g034290 28.118 441 286 11 33 468 75 489 7.15e-52 182
MS.gene004369.t1 MTR_3g090130 27.824 478 303 14 7 468 4 455 6.03e-51 179
MS.gene004369.t1 MTR_3g079570 29.062 437 273 13 33 463 79 484 8.43e-51 180
MS.gene004369.t1 MTR_2g449850 28.306 431 269 13 51 467 56 460 1.27e-50 179
MS.gene004369.t1 MTR_1g021010 31.963 438 258 14 33 463 76 480 1.29e-50 179
MS.gene004369.t1 MTR_4g089155 29.412 442 287 15 32 463 53 479 5.32e-50 177
MS.gene004369.t1 MTR_3g090150 29.890 455 268 16 36 468 37 462 1.04e-49 176
MS.gene004369.t1 MTR_7g021130 28.689 488 291 16 4 468 3 456 1.81e-49 176
MS.gene004369.t1 MTR_3g079560 29.318 440 269 14 33 463 78 484 6.38e-49 175
MS.gene004369.t1 MTR_2g063000 27.252 444 268 12 31 468 37 431 9.27e-49 173
MS.gene004369.t1 MTR_1g020090 31.066 441 255 14 33 463 90 491 4.74e-48 172
MS.gene004369.t1 MTR_1g095560 27.308 509 298 17 9 468 2 487 1.27e-47 171
MS.gene004369.t1 MTR_0003s0560 28.444 450 271 12 33 468 74 486 5.18e-47 169
MS.gene004369.t1 MTR_3g079600 28.507 442 273 13 33 463 76 485 1.14e-46 169
MS.gene004369.t1 MTR_3g079620 28.475 446 273 15 33 463 78 492 5.10e-46 167
MS.gene004369.t1 MTR_3g079630 28.412 447 272 13 33 463 78 492 1.39e-44 163
MS.gene004369.t1 MTR_1g021020 29.571 443 261 15 33 463 78 481 2.66e-43 159
MS.gene004369.t1 MTR_2g095750 27.517 447 281 12 35 468 84 500 1.14e-42 158
MS.gene004369.t1 MTR_2g095730 28.233 464 268 16 30 466 70 495 2.28e-41 154
MS.gene004369.t1 MTR_1g040370 26.802 444 244 12 40 468 37 414 2.76e-40 149
MS.gene004369.t1 MTR_1g050545 26.667 435 256 11 49 462 85 477 3.29e-40 151
MS.gene004369.t1 MTR_4g057585 25.943 424 274 8 49 463 98 490 3.33e-39 148
MS.gene004369.t1 MTR_4g057595 25.943 424 274 8 49 463 99 491 4.99e-39 148
MS.gene004369.t1 MTR_4g057600 24.941 425 283 8 49 466 97 492 3.19e-37 142
MS.gene004369.t1 MTR_2g018650 58.120 117 47 1 316 432 8 122 5.07e-37 132
MS.gene004369.t1 MTR_4g057570 25.459 436 263 10 50 463 92 487 9.49e-37 141
MS.gene004369.t1 MTR_1g094155 25.995 427 268 14 49 462 92 483 1.66e-36 141
MS.gene004369.t1 MTR_1g102130 27.354 446 235 17 49 468 107 489 4.81e-36 139
MS.gene004369.t1 MTR_3g034290 31.154 260 168 6 33 289 75 326 1.86e-34 133
MS.gene004369.t1 MTR_5g097180 23.992 471 301 13 13 468 9 437 1.12e-32 129
MS.gene004369.t1 MTR_0003s0560 30.769 260 167 7 33 289 74 323 4.18e-30 120
MS.gene004369.t1 MTR_2g095710 24.419 430 250 15 62 468 106 483 8.12e-28 115
MS.gene004369.t1 MTR_4g084710 24.312 436 273 12 49 468 45 439 1.45e-27 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004369.t1 AT5G09640 53.731 469 202 9 10 468 2 465 3.51e-172 492
MS.gene004369.t1 AT2G22920 54.751 442 169 6 30 468 22 435 6.16e-169 483
MS.gene004369.t1 AT1G33540 53.515 441 201 4 30 468 26 464 8.24e-168 481
MS.gene004369.t1 AT2G22970 53.409 440 176 3 30 468 22 433 1.01e-167 479
MS.gene004369.t1 AT3G10450 52.809 445 181 4 25 468 21 437 3.17e-166 476
MS.gene004369.t1 AT1G73270 54.318 440 172 3 30 468 30 441 7.05e-164 470
MS.gene004369.t1 AT1G73270 54.318 440 172 3 30 468 30 441 7.66e-164 470
MS.gene004369.t1 AT5G36180 54.586 447 170 5 25 468 25 441 8.30e-164 470
MS.gene004369.t1 AT2G23000 54.091 440 177 4 30 468 22 437 1.52e-163 469
MS.gene004369.t1 AT2G23000 54.091 440 177 4 30 468 22 437 1.52e-163 469
MS.gene004369.t1 AT1G73280 51.496 468 195 5 5 468 2 441 6.04e-163 468
MS.gene004369.t1 AT1G73300 53.258 445 179 4 25 468 25 441 1.61e-161 464
MS.gene004369.t1 AT1G73300 53.258 445 179 4 25 468 25 441 1.61e-161 464
MS.gene004369.t1 AT2G22980 52.928 444 174 6 27 468 20 430 1.63e-161 464
MS.gene004369.t1 AT2G22990 53.182 440 179 5 30 468 20 433 2.19e-160 461
MS.gene004369.t1 AT1G73290 52.360 445 181 5 25 468 24 438 1.06e-159 459
MS.gene004369.t1 AT3G12203 50.655 458 193 4 12 468 12 437 2.57e-159 458
MS.gene004369.t1 AT1G73310 53.258 445 179 4 25 468 25 441 7.43e-159 457
MS.gene004369.t1 AT3G12203 50.901 444 188 3 26 468 2 416 1.01e-158 456
MS.gene004369.t1 AT2G23010 50.870 460 197 5 10 468 6 437 3.47e-158 455
MS.gene004369.t1 AT1G33540 53.095 420 193 4 51 468 4 421 5.40e-158 454
MS.gene004369.t1 AT2G22970 53.125 416 167 2 30 444 22 410 6.59e-158 455
MS.gene004369.t1 AT2G22970 53.140 414 166 2 30 442 22 408 2.02e-157 452
MS.gene004369.t1 AT2G23010 50.435 460 199 5 10 468 6 437 1.34e-156 451
MS.gene004369.t1 AT3G10450 52.029 419 172 4 51 468 4 394 2.59e-154 444
MS.gene004369.t1 AT3G10450 52.029 419 173 3 25 442 21 412 2.52e-153 442
MS.gene004369.t1 AT2G22990 51.591 440 177 6 30 468 20 424 5.81e-152 439
MS.gene004369.t1 AT3G12240 48.879 446 199 3 24 468 19 436 4.65e-150 435
MS.gene004369.t1 AT2G22980 52.153 418 166 5 27 442 20 405 1.27e-149 432
MS.gene004369.t1 AT2G22980 50.808 433 177 5 27 455 20 420 2.37e-149 433
MS.gene004369.t1 AT3G12220 49.661 443 194 3 27 468 21 435 1.25e-147 428
MS.gene004369.t1 AT2G22920 52.868 401 159 5 30 427 22 395 4.42e-147 426
MS.gene004369.t1 AT2G22990 50.930 430 182 5 30 455 20 424 6.61e-147 427
MS.gene004369.t1 AT2G22990 51.932 414 173 4 30 442 20 408 9.17e-147 426
MS.gene004369.t1 AT3G12230 48.533 443 199 3 27 468 21 435 1.29e-145 423
MS.gene004369.t1 AT3G12203 50.000 408 174 3 62 468 1 379 2.95e-142 412
MS.gene004369.t1 AT3G10450 51.145 393 164 3 51 442 4 369 2.24e-141 410
MS.gene004369.t1 AT1G73310 51.961 408 168 3 25 431 25 405 3.48e-141 412
MS.gene004369.t1 AT1G73290 51.238 404 167 4 25 427 24 398 1.58e-139 407
MS.gene004369.t1 AT3G12220 48.926 419 185 3 51 468 15 405 6.56e-137 400
MS.gene004369.t1 AT4G12910 39.300 486 259 7 10 463 11 492 6.72e-124 370
MS.gene004369.t1 AT5G36180 50.407 369 150 5 103 468 1 339 2.56e-117 348
MS.gene004369.t1 AT5G36180 50.407 369 150 5 103 468 1 339 2.56e-117 348
MS.gene004369.t1 AT1G73280 49.046 367 158 4 103 468 1 339 3.47e-117 347
MS.gene004369.t1 AT1G73280 48.320 387 169 4 5 387 2 361 5.87e-117 348
MS.gene004369.t1 AT3G25420 36.437 494 273 9 11 468 6 494 2.42e-114 345
MS.gene004369.t1 AT2G22990 51.014 345 142 5 125 468 1 319 3.01e-113 337
MS.gene004369.t1 AT2G22980 48.767 365 151 6 27 387 20 352 2.96e-112 335
MS.gene004369.t1 AT2G22990 50.435 345 144 5 125 468 1 319 8.43e-112 333
MS.gene004369.t1 AT2G22970 50.470 319 131 1 30 348 22 313 2.69e-109 327
MS.gene004369.t1 AT1G73280 49.123 342 145 4 128 468 1 314 8.24e-108 322
MS.gene004369.t1 AT2G22920 51.250 320 128 4 30 348 22 314 2.07e-107 322
MS.gene004369.t1 AT5G36180 49.709 344 141 4 103 443 1 315 2.59e-107 321
MS.gene004369.t1 AT4G12910 38.085 449 242 7 10 426 11 455 7.08e-105 320
MS.gene004369.t1 AT1G73310 47.720 329 144 3 104 431 2 303 2.14e-97 296
MS.gene004369.t1 AT2G22980 48.287 321 132 5 125 443 1 289 4.97e-97 294
MS.gene004369.t1 AT5G36180 48.624 327 136 4 103 426 1 298 1.43e-96 295
MS.gene004369.t1 AT2G22920 52.837 282 102 6 190 468 3 256 8.29e-96 290
MS.gene004369.t1 AT1G73290 53.933 267 120 2 25 290 24 288 1.70e-95 291
MS.gene004369.t1 AT3G12220 54.741 232 104 1 27 257 21 252 5.67e-78 248
MS.gene004369.t1 AT3G12220 53.793 145 66 1 324 468 240 383 2.61e-47 168
MS.gene004369.t1 AT5G23210 29.913 458 296 12 27 468 46 494 6.59e-60 204
MS.gene004369.t1 AT3G52010 29.167 456 268 13 32 468 64 483 7.16e-60 204
MS.gene004369.t1 AT3G52000 28.348 448 278 12 32 468 63 478 7.26e-60 204
MS.gene004369.t1 AT4G30810 29.345 443 287 13 33 468 38 461 1.31e-59 203
MS.gene004369.t1 AT2G05850 29.515 454 270 15 32 468 63 483 1.35e-59 203
MS.gene004369.t1 AT4G15100 30.752 452 285 13 26 463 38 475 1.43e-59 203
MS.gene004369.t1 AT3G52020 30.454 463 266 14 25 468 72 497 1.02e-58 201
MS.gene004369.t1 AT5G08260 30.942 446 290 14 32 468 39 475 4.36e-58 199
MS.gene004369.t1 AT4G30610 31.180 449 276 16 33 468 33 461 5.65e-57 196
MS.gene004369.t1 AT2G22960 62.590 139 51 1 330 468 47 184 1.96e-56 186
MS.gene004369.t1 AT2G35780 30.337 445 277 14 33 468 30 450 1.10e-55 192
MS.gene004369.t1 AT2G35770 30.000 470 290 12 12 468 14 457 2.78e-55 191
MS.gene004369.t1 AT3G17180 28.176 433 285 14 49 468 55 474 8.84e-55 190
MS.gene004369.t1 AT2G24010 30.512 449 272 16 32 468 30 450 1.20e-54 189
MS.gene004369.t1 AT1G43780 29.103 457 280 14 32 468 37 469 3.41e-53 186
MS.gene004369.t1 AT3G63470 27.828 442 288 9 32 468 81 496 4.88e-53 186
MS.gene004369.t1 AT1G61130 30.425 447 275 16 32 464 31 455 5.78e-53 185
MS.gene004369.t1 AT3G02110 29.581 453 282 15 33 468 38 470 6.91e-53 185
MS.gene004369.t1 AT3G07990 28.698 453 276 15 33 468 34 456 2.37e-52 183
MS.gene004369.t1 AT1G11080 31.087 460 277 15 26 464 44 484 3.07e-52 184
MS.gene004369.t1 AT1G28110 29.847 459 266 15 33 468 32 457 3.07e-52 183
MS.gene004369.t1 AT1G28110 29.847 459 266 15 33 468 32 457 3.07e-52 183
MS.gene004369.t1 AT1G11080 31.087 460 277 15 26 464 44 484 3.32e-52 184
MS.gene004369.t1 AT2G24000 29.213 445 291 13 33 468 31 460 3.13e-51 180
MS.gene004369.t1 AT2G33530 29.268 451 281 16 33 468 34 461 1.55e-50 178
MS.gene004369.t1 AT2G24010 29.310 464 273 17 32 468 30 465 1.25e-49 176
MS.gene004369.t1 AT2G12480 27.679 448 291 12 32 468 31 456 1.49e-48 173
MS.gene004369.t1 AT5G42240 28.913 460 276 15 32 468 32 463 1.64e-48 173
MS.gene004369.t1 AT5G23210 29.710 414 267 11 27 424 46 451 2.82e-48 172
MS.gene004369.t1 AT5G23210 28.500 400 262 11 85 468 8 399 4.45e-46 165
MS.gene004369.t1 AT2G12480 27.902 448 266 13 32 468 31 432 5.08e-46 166
MS.gene004369.t1 AT2G24010 27.572 486 278 17 32 468 1 461 2.39e-45 164
MS.gene004369.t1 AT5G42230 28.665 457 281 15 32 468 28 459 3.39e-45 164
MS.gene004369.t1 AT5G42230 28.665 457 281 15 32 468 28 459 3.39e-45 164
MS.gene004369.t1 AT5G23210 28.321 399 262 11 86 468 8 398 3.46e-45 162
MS.gene004369.t1 AT2G12480 27.679 448 265 13 32 468 31 430 4.48e-45 163
MS.gene004369.t1 AT2G12480 27.617 449 265 14 32 468 31 431 2.22e-43 159
MS.gene004369.t1 AT3G17180 27.551 392 258 14 49 427 55 433 3.34e-43 158
MS.gene004369.t1 AT5G22980 27.546 432 262 13 49 466 100 494 1.24e-41 155
MS.gene004369.t1 AT3G45010 26.218 431 279 12 45 466 99 499 9.61e-41 153
MS.gene004369.t1 AT3G10410 26.207 435 258 13 49 463 101 492 9.07e-40 150
MS.gene004369.t1 AT5G23210 27.887 355 233 10 86 424 8 355 1.39e-33 130
MS.gene004369.t1 AT2G24010 29.830 352 195 15 32 358 1 325 9.90e-31 122
MS.gene004369.t1 AT3G56540 36.585 164 97 3 25 187 72 229 2.01e-28 113
MS.gene004369.t1 AT1G15000 22.811 434 279 13 48 468 36 426 2.08e-24 105
MS.gene004369.t1 AT5G22960 29.091 165 92 2 48 212 35 174 6.85e-21 90.5
MS.gene004369.t1 AT2G24000 25.260 289 201 8 186 468 118 397 9.16e-20 91.7

Find 108 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCATTTGATTGAAATTT+TGG 0.048032 2.4:+67627055 None:intergenic
CAAATTTCCTTATGGGATTT+TGG 0.115996 2.4:-67625887 MS.gene004369:CDS
CAAAATCCCATAAGGAAATT+TGG 0.197966 2.4:+67625888 None:intergenic
ATAATTTCTTGAACAATTAC+TGG 0.233098 2.4:+67626995 None:intergenic
CAGATTTGTATTGAGGAATA+TGG 0.256288 2.4:+67626077 None:intergenic
TACAGTCCGGGTCTAAAATA+TGG 0.283742 2.4:+67626157 None:intergenic
GAGTTATCCAAATTTCCTTA+TGG 0.290457 2.4:-67625895 MS.gene004369:CDS
CGACATGGCAATGCCATTCT+TGG 0.303823 2.4:-67625388 MS.gene004369:CDS
TTTCTTAAGGGGTACCTGTT+GGG 0.310979 2.4:-67626595 MS.gene004369:intron
AGGTCCACTTGAATTTAAAA+AGG 0.321418 2.4:-67627533 MS.gene004369:CDS
GCTAAGTTTAGTTCATGTTA+TGG 0.324519 2.4:-67628445 MS.gene004369:CDS
TCCATAGTACACTCTCTTCC+TGG 0.325258 2.4:-67628358 MS.gene004369:CDS
ACATGATAATCATAGCTGTT+AGG 0.338091 2.4:+67625556 None:intergenic
GCATTCGTTCACTAATTTAC+AGG 0.355008 2.4:-67625516 MS.gene004369:intron
TTGGGGAATGCTGCAACAAC+TGG 0.358085 2.4:-67626577 MS.gene004369:CDS
AGGATGATCCTCTCATACTA+TGG 0.360528 2.4:-67628131 MS.gene004369:CDS
TCTTGAACAATTACTGGAAC+AGG 0.369558 2.4:+67627001 None:intergenic
ACTATTCCATTGTTGATGAT+TGG 0.372894 2.4:-67625338 MS.gene004369:CDS
AGTTATCCAAATTTCCTTAT+GGG 0.374392 2.4:-67625894 MS.gene004369:CDS
CCTGGTTGCTCTGCTTTGTC+TGG 0.374838 2.4:-67628096 MS.gene004369:CDS
TGATCCTGAGGCCACATTCA+TGG 0.384490 2.4:-67627483 MS.gene004369:CDS
CATACAAATGACCAAGTTGC+AGG 0.404512 2.4:-67625306 MS.gene004369:CDS
ACCGAGCTCTTCAATTTCTC+AGG 0.411467 2.4:-67627229 MS.gene004369:CDS
TTCTTAAGGGGTACCTGTTG+GGG 0.415695 2.4:-67626594 MS.gene004369:intron
GACATGGCAATGCCATTCTT+GGG 0.424322 2.4:-67625387 MS.gene004369:CDS
CATGATAATCATAGCTGTTA+GGG 0.426927 2.4:+67625557 None:intergenic
TTTGCGAACATTATCGTCAT+TGG 0.430154 2.4:+67625863 None:intergenic
TTGTTGCAGCATTCCCCAAC+AGG 0.431521 2.4:+67626581 None:intergenic
GCAGATTTGCCTGCTTCCAC+AGG 0.434474 2.4:-67627308 MS.gene004369:CDS
GCTTTAATATACCTCATAAG+AGG 0.437793 2.4:-67625585 MS.gene004369:CDS
ATGTTGGAGTTGGTGAGAAA+GGG 0.442116 2.4:-67628200 MS.gene004369:CDS
GCAATTTCAGATTTGTATTG+AGG 0.443024 2.4:+67626070 None:intergenic
TTGGTGGCAGGAAATGAAAA+AGG 0.448861 2.4:-67626886 MS.gene004369:intron
TATGTTGGAGTTGGTGAGAA+AGG 0.449163 2.4:-67628201 MS.gene004369:CDS
AGTATGAGAGGATCATCCTT+TGG 0.453644 2.4:+67628135 None:intergenic
AGTCCCATTCCATGAGCAAA+AGG 0.456013 2.4:+67626532 None:intergenic
GACCCAGTTTCAAGCACAAA+AGG 0.460216 2.4:+67628319 None:intergenic
GGAAGTGATCCTTGGAATCC+AGG 0.464903 2.4:+67628340 None:intergenic
TCATGTAAATCTCAGTAGAA+AGG 0.469015 2.4:-67625539 MS.gene004369:CDS
GACACACAGCTCCGGAGTAC+CGG 0.470262 2.4:-67624944 MS.gene004369:CDS
TCAAATGAAGTTTACATTGG+TGG 0.474459 2.4:-67627043 MS.gene004369:CDS
TATTTCTTGATTAGGTATGT+TGG 0.476072 2.4:-67628216 MS.gene004369:intron
CTATGGCTCACTGGTGGACC+TGG 0.476423 2.4:-67628114 MS.gene004369:CDS
ACTTATGCTACAACAGAGTC+TGG 0.478380 2.4:-67627281 MS.gene004369:CDS
CATGTAAATCTCAGTAGAAA+GGG 0.479087 2.4:-67625538 MS.gene004369:CDS
TCCAGGAAGAGAGTGTACTA+TGG 0.484645 2.4:+67628357 None:intergenic
ATTGGTGGTGATTCATACTC+TGG 0.487605 2.4:-67627028 MS.gene004369:CDS
CATGCTTGTGTCCCCAAGAA+TGG 0.489406 2.4:+67625375 None:intergenic
TAAGTAAAGCCTGTGGAAGC+AGG 0.489762 2.4:+67627299 None:intergenic
TTGATTAGGTATGTTGGAGT+TGG 0.490410 2.4:-67628210 MS.gene004369:intron
GCATTACTCGACAGCTTAAG+TGG 0.491599 2.4:+67626046 None:intergenic
GCACAAAAGGAAGTGATCCT+TGG 0.496794 2.4:+67628332 None:intergenic
AGTTGCAAATGTCATTCCAT+TGG 0.501840 2.4:+67625113 None:intergenic
TTGTCCATGAATGTGGCCTC+AGG 0.505965 2.4:+67627479 None:intergenic
CAAACCATGGATAAATCTCC+AGG 0.506203 2.4:-67626860 MS.gene004369:intron
CAATCAAATGAAGTTTACAT+TGG 0.508546 2.4:-67627046 MS.gene004369:CDS
CATTGGTCCAAAATCCCATA+AGG 0.515845 2.4:+67625880 None:intergenic
CACTCTCTTCCTGGATTCCA+AGG 0.520558 2.4:-67628349 MS.gene004369:CDS
CCTCTCATACTATGGCTCAC+TGG 0.524416 2.4:-67628123 MS.gene004369:CDS
TGTGGCCTCAGGATCAGATT+AGG 0.531982 2.4:+67627490 None:intergenic
AATTACGTCGTATAGTTCAT+CGG 0.535501 2.4:+67626504 None:intergenic
GGGTGGAGGACACACAGCTC+CGG 0.539548 2.4:-67624952 MS.gene004369:CDS
TCAACAAGCCACATACAGTC+CGG 0.542049 2.4:+67626144 None:intergenic
TCCGGGTCTAAAATATGGAA+TGG 0.545216 2.4:+67626162 None:intergenic
GTTTAGTTCATGTTATGGAA+AGG 0.546320 2.4:-67628440 MS.gene004369:CDS
TAAATCTTTGTAGTGGTTGG+TGG 0.548615 2.4:-67627083 MS.gene004369:intron
TGCTTAATGAGAGATCTCCT+TGG 0.550010 2.4:+67626108 None:intergenic
TCATTTCAACTTGCAAAATG+TGG 0.551003 2.4:-67628382 MS.gene004369:CDS
TGTTGGAGTTGGTGAGAAAG+GGG 0.551974 2.4:-67628199 MS.gene004369:CDS
TGCAGGGAAGTGTTGCGAAA+TGG 0.556068 2.4:-67625618 MS.gene004369:intron
ATTTGTATTGAGGAATATGG+AGG 0.556728 2.4:+67626080 None:intergenic
TCCTGAGAAATTGAAGAGCT+CGG 0.556991 2.4:+67627228 None:intergenic
TTAGGGATATCCCTCTTATG+AGG 0.558242 2.4:+67625574 None:intergenic
CTTACCTGGAGATTTATCCA+TGG 0.564764 2.4:+67626856 None:intergenic
CTCATACTATGGCTCACTGG+TGG 0.567761 2.4:-67628120 MS.gene004369:CDS
AGCTCTTCAATTTCTCAGGA+AGG 0.573080 2.4:-67627225 MS.gene004369:intron
CATTCCTTAGGCCGGTACTC+CGG 0.579505 2.4:+67624933 None:intergenic
AGCTCCGGAGTACCGGCCTA+AGG 0.583024 2.4:-67624937 MS.gene004369:CDS
CCAGACAAAGCAGAGCAACC+AGG 0.587330 2.4:+67628096 None:intergenic
GAATGCTGCAACAACTGGAA+AGG 0.587964 2.4:-67626572 MS.gene004369:CDS
GTCCCATTCCATGAGCAAAA+GGG 0.589244 2.4:+67626533 None:intergenic
CTAACCTTTGTCCATGAATG+TGG 0.590461 2.4:+67627472 None:intergenic
AGATATGAGTTCATTTGATG+AGG 0.595549 2.4:-67626289 MS.gene004369:intron
ATTGTTCAAGAAATTATACA+AGG 0.595697 2.4:-67626989 MS.gene004369:intron
CAAAGCACTGCACATACGTA+AGG 0.596898 2.4:-67625844 MS.gene004369:intron
CATTACTCGACAGCTTAAGT+GGG 0.597053 2.4:+67626047 None:intergenic
CAACAAGCCACATACAGTCC+GGG 0.601579 2.4:+67626145 None:intergenic
CCAGTGAGCCATAGTATGAG+AGG 0.603687 2.4:+67628123 None:intergenic
CTTTAATATACCTCATAAGA+GGG 0.608963 2.4:-67625584 MS.gene004369:CDS
TGTCAAAACATTCCTTAGGC+CGG 0.611801 2.4:+67624925 None:intergenic
TATGTGAATACCCTGCAACT+TGG 0.618197 2.4:+67625295 None:intergenic
ACTATTTCATTGAATCAGAG+AGG 0.626950 2.4:-67628161 MS.gene004369:CDS
CATTCTTGGGGACACAAGCA+TGG 0.627104 2.4:-67625374 MS.gene004369:CDS
CTGTCTCCAATCATCAACAA+TGG 0.629618 2.4:+67625332 None:intergenic
AACATGTCAAAACATTCCTT+AGG 0.633010 2.4:+67624921 None:intergenic
ACATGGCAATGCCATTCTTG+GGG 0.633060 2.4:-67625386 MS.gene004369:CDS
ATACAAATGACCAAGTTGCA+GGG 0.634663 2.4:-67625305 MS.gene004369:intron
TTTGTATTGAGGAATATGGA+GGG 0.634757 2.4:+67626081 None:intergenic
TACACAAGGACTTACTCCAA+TGG 0.638002 2.4:-67625129 MS.gene004369:CDS
TAACAGTGGCGATCACGACA+TGG 0.642097 2.4:-67625403 MS.gene004369:intron
TGAATCAGAGAGGAATCCAA+AGG 0.653936 2.4:-67628151 MS.gene004369:CDS
GCTTGCCTAATCTGATCCTG+AGG 0.654203 2.4:-67627495 MS.gene004369:CDS
AATGACATTTGCAACTGTGA+AGG 0.656552 2.4:-67625106 MS.gene004369:intron
GAGGCCACATTCATGGACAA+AGG 0.670075 2.4:-67627476 MS.gene004369:intron
TGTAGCATAAGTAAAGCCTG+TGG 0.704021 2.4:+67627292 None:intergenic
ATTACTCGACAGCTTAAGTG+GGG 0.708249 2.4:+67626048 None:intergenic
TTGTTGATGATTGGAGACAG+TGG 0.718131 2.4:-67625329 MS.gene004369:CDS
TCTTCGAGTATAGATACACA+AGG 0.736360 2.4:-67625143 MS.gene004369:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTCGATAAAATAAA+TGG + chr2.4:67627088-67627107 None:intergenic 10.0%
!! TAATTATTCACAAATTAAAT+TGG - chr2.4:67626433-67626452 MS.gene004369:intron 10.0%
!! AATTACAGTAACTAAATTTA+TGG + chr2.4:67628069-67628088 None:intergenic 15.0%
!! ACTAATAAAAAATATCTTTG+TGG - chr2.4:67625446-67625465 MS.gene004369:intron 15.0%
!!! ATATTTGTAATGATTTTTCT+TGG - chr2.4:67625986-67626005 MS.gene004369:intron 15.0%
!!! TAATTGCAACTTTAATTTAT+AGG - chr2.4:67625352-67625371 MS.gene004369:CDS 15.0%
!! ACATAAGAACAATATAAGTT+TGG + chr2.4:67627538-67627557 None:intergenic 20.0%
!! ACCAATGAAAAAAATATTTG+AGG - chr2.4:67625383-67625402 MS.gene004369:CDS 20.0%
!! AGTAATGATTCTATATCTAA+AGG + chr2.4:67628329-67628348 None:intergenic 20.0%
!! ATAATTTCTTGAACAATTAC+TGG + chr2.4:67626346-67626365 None:intergenic 20.0%
!! ATTGTTCAAGAAATTATACA+AGG - chr2.4:67626349-67626368 MS.gene004369:CDS 20.0%
!! CATATTCATATTTCTTGATT+AGG - chr2.4:67625114-67625133 MS.gene004369:CDS 20.0%
!! GTTTCTTTAATCTATGTTAT+TGG - chr2.4:67625078-67625097 MS.gene004369:intron 20.0%
!! TCGTATATATGTATAAATCT+CGG - chr2.4:67627378-67627397 MS.gene004369:intron 20.0%
!! TTATTCACAAATTAAATTGG+TGG - chr2.4:67626436-67626455 MS.gene004369:intron 20.0%
!!! ACTTCATTTGATTGAAATTT+TGG + chr2.4:67626286-67626305 None:intergenic 20.0%
!!! ATATTTTATTGATGCTTCAT+AGG + chr2.4:67626932-67626951 None:intergenic 20.0%
!!! ATGAAATGTTGTTTTCTTAA+GGG - chr2.4:67626731-67626750 MS.gene004369:intron 20.0%
!!! ATTGATTGATCAAATTTTCA+GGG - chr2.4:67628366-67628385 MS.gene004369:CDS 20.0%
!!! ATTTGCAATTTTTATACACT+TGG - chr2.4:67625473-67625492 MS.gene004369:intron 20.0%
!!! TATTGATTGATCAAATTTTC+AGG - chr2.4:67628365-67628384 MS.gene004369:CDS 20.0%
!!! TCCTCAAATATTTTTTTCAT+TGG + chr2.4:67625387-67625406 None:intergenic 20.0%
!!! TTATGTATTTCTAATAACAG+TGG - chr2.4:67627921-67627940 MS.gene004369:intron 20.0%
! AAATGAAAGCAATAGAAACT+AGG + chr2.4:67626148-67626167 None:intergenic 25.0%
! AATGAAAGCAATAGAAACTA+GGG + chr2.4:67626147-67626166 None:intergenic 25.0%
! ACATAATGCCTTTATGTTAT+AGG - chr2.4:67628288-67628307 MS.gene004369:intron 25.0%
! AGTTATCCAAATTTCCTTAT+GGG - chr2.4:67627444-67627463 MS.gene004369:intron 25.0%
! CAATCAAATGAAGTTTACAT+TGG - chr2.4:67626292-67626311 MS.gene004369:CDS 25.0%
! CATTGCATTGCATAAATATA+CGG + chr2.4:67627325-67627344 None:intergenic 25.0%
! CTTTAATATACCTCATAAGA+GGG - chr2.4:67627754-67627773 MS.gene004369:intron 25.0%
! TACACATTCCTATAACATAA+AGG + chr2.4:67628299-67628318 None:intergenic 25.0%
! TATTTCTTGATTAGGTATGT+TGG - chr2.4:67625122-67625141 MS.gene004369:CDS 25.0%
! TCCTTGCAAAAAAATTGTAA+TGG - chr2.4:67626984-67627003 MS.gene004369:intron 25.0%
! TTAAAAAGGAGGAATACAAT+GGG - chr2.4:67625819-67625838 MS.gene004369:intron 25.0%
! TTGAAACTGAAAAATTTCCA+AGG - chr2.4:67627213-67627232 MS.gene004369:intron 25.0%
! TTGTGAATAAATCTTTGTAG+TGG - chr2.4:67626248-67626267 MS.gene004369:intron 25.0%
! TTTAAAAAGGAGGAATACAA+TGG - chr2.4:67625818-67625837 MS.gene004369:intron 25.0%
! TTTGTTGCAGTTATATCAAT+CGG - chr2.4:67626200-67626219 MS.gene004369:intron 25.0%
!! AAGAAGCTTCTAAGAATAAA+TGG + chr2.4:67627568-67627587 None:intergenic 25.0%
!! AATTTGGATAACTCTAAAAG+TGG + chr2.4:67627437-67627456 None:intergenic 25.0%
!! TTGTAATGATTTTTCTTGGT+AGG - chr2.4:67625990-67626009 MS.gene004369:intron 25.0%
!!! AATGTTTTGACATGTTTAGT+AGG - chr2.4:67628424-67628443 MS.gene004369:CDS 25.0%
!!! CATGAAATGTTGTTTTCTTA+AGG - chr2.4:67626730-67626749 MS.gene004369:intron 25.0%
!!! TAAAGCCGAAATTTTTTACT+CGG + chr2.4:67625918-67625937 None:intergenic 25.0%
!!! TCCATTACAATTTTTTTGCA+AGG + chr2.4:67626988-67627007 None:intergenic 25.0%
!!! TGAAATGTTGTTTTCTTAAG+GGG - chr2.4:67626732-67626751 MS.gene004369:intron 25.0%
!!! TGTATGATCTATTTCTATTG+AGG + chr2.4:67628271-67628290 None:intergenic 25.0%
AAACTTTGCTTATGTTTCGA+GGG + chr2.4:67625579-67625598 None:intergenic 30.0%
AACATGTCAAAACATTCCTT+AGG + chr2.4:67628420-67628439 None:intergenic 30.0%
AATTACGTCGTATAGTTCAT+CGG + chr2.4:67626837-67626856 None:intergenic 30.0%
ACATGATAATCATAGCTGTT+AGG + chr2.4:67627785-67627804 None:intergenic 30.0%
ACTATTTCATTGAATCAGAG+AGG - chr2.4:67625177-67625196 MS.gene004369:intron 30.0%
AGATATGAGTTCATTTGATG+AGG - chr2.4:67627049-67627068 MS.gene004369:CDS 30.0%
AGGTCCACTTGAATTTAAAA+AGG - chr2.4:67625805-67625824 MS.gene004369:intron 30.0%
ATTTGTATTGAGGAATATGG+AGG + chr2.4:67627261-67627280 None:intergenic 30.0%
CAAAATCCCATAAGGAAATT+TGG + chr2.4:67627453-67627472 None:intergenic 30.0%
CAACTATATATGTAAGAGCT+TGG - chr2.4:67627687-67627706 MS.gene004369:intron 30.0%
CAGATTTGTATTGAGGAATA+TGG + chr2.4:67627264-67627283 None:intergenic 30.0%
CATGATAATCATAGCTGTTA+GGG + chr2.4:67627784-67627803 None:intergenic 30.0%
CATGTAAATCTCAGTAGAAA+GGG - chr2.4:67627800-67627819 MS.gene004369:intron 30.0%
GAATAAATCTTTGTAGTGGT+TGG - chr2.4:67626252-67626271 MS.gene004369:intron 30.0%
GAGTTATCCAAATTTCCTTA+TGG - chr2.4:67627443-67627462 MS.gene004369:intron 30.0%
GCAATTTCAGATTTGTATTG+AGG + chr2.4:67627271-67627290 None:intergenic 30.0%
GCTTTAATATACCTCATAAG+AGG - chr2.4:67627753-67627772 MS.gene004369:intron 30.0%
GTTGTCCGAGTAAAAAATTT+CGG - chr2.4:67625910-67625929 MS.gene004369:intron 30.0%
TAAACTTTGCTTATGTTTCG+AGG + chr2.4:67625580-67625599 None:intergenic 30.0%
TAGATATCAATCTGAACTGT+AGG + chr2.4:67625709-67625728 None:intergenic 30.0%
TATACAAGGTACAAAACTCA+TGG - chr2.4:67626363-67626382 MS.gene004369:intron 30.0%
TATTGCAAATGTCATTCCAT+TGG + chr2.4:67628162-67628181 None:intergenic 30.0%
TCAAATGAAGTTTACATTGG+TGG - chr2.4:67626295-67626314 MS.gene004369:CDS 30.0%
TCATGTAAATCTCAGTAGAA+AGG - chr2.4:67627799-67627818 MS.gene004369:intron 30.0%
TCATTTCAACTTGCAAAATG+TGG - chr2.4:67624956-67624975 MS.gene004369:intron 30.0%
TCCACTTGAATTTAAAAAGG+AGG - chr2.4:67625808-67625827 MS.gene004369:intron 30.0%
TCCTTGAAGAATATTCAACT+TGG + chr2.4:67628133-67628152 None:intergenic 30.0%
TTTGTATTGAGGAATATGGA+GGG + chr2.4:67627260-67627279 None:intergenic 30.0%
! ACCAAGTTGAATATTCTTCA+AGG - chr2.4:67628129-67628148 MS.gene004369:CDS 30.0%
! CAAATTTCCTTATGGGATTT+TGG - chr2.4:67627451-67627470 MS.gene004369:intron 30.0%
! CAGTAAACCAACAATCATTT+TGG + chr2.4:67625531-67625550 None:intergenic 30.0%
! CTTTTGTGATTTAGTCTACA+TGG - chr2.4:67625742-67625761 MS.gene004369:intron 30.0%
! GCTAAGTTTAGTTCATGTTA+TGG - chr2.4:67624893-67624912 MS.gene004369:CDS 30.0%
! GGTAATTGCAACTTTACTTT+TGG - chr2.4:67625281-67625300 MS.gene004369:intron 30.0%
! GTTTAGTTCATGTTATGGAA+AGG - chr2.4:67624898-67624917 MS.gene004369:CDS 30.0%
! TCCTCCTTTTTAAATTCAAG+TGG + chr2.4:67625812-67625831 None:intergenic 30.0%
!! ACTATTCCATTGTTGATGAT+TGG - chr2.4:67628000-67628019 MS.gene004369:intron 30.0%
AACAATCATTTGGTCTCGAT+TGG - chr2.4:67625329-67625348 MS.gene004369:CDS 35.0%
AAGGATCATTCTCTCATGTT+AGG - chr2.4:67626512-67626531 MS.gene004369:CDS 35.0%
AATGACATTTGCAACTGTGA+AGG - chr2.4:67628232-67628251 MS.gene004369:intron 35.0%
ACATTCATGGACAAAGGTTA+GGG - chr2.4:67625868-67625887 MS.gene004369:CDS 35.0%
ACTCGGACAACAATAATATG+TGG + chr2.4:67625901-67625920 None:intergenic 35.0%
ACTGAAACTTGAAATGTTGC+AGG - chr2.4:67625785-67625804 MS.gene004369:intron 35.0%
AGCCTGTGAAAATAGAAGAT+TGG + chr2.4:67627141-67627160 None:intergenic 35.0%
AGTTGCAAATGTCATTCCAT+TGG + chr2.4:67628228-67628247 None:intergenic 35.0%
ATACAAATGACCAAGTTGCA+GGG - chr2.4:67628033-67628052 MS.gene004369:intron 35.0%
ATATACGATGATGCAGCAAA+TGG + chr2.4:67627366-67627385 None:intergenic 35.0%
GCATTCGTTCACTAATTTAC+AGG - chr2.4:67627822-67627841 MS.gene004369:intron 35.0%
GCATTGAGCAAACAATCATT+TGG - chr2.4:67625319-67625338 MS.gene004369:CDS 35.0%
TAAATCTTTGTAGTGGTTGG+TGG - chr2.4:67626255-67626274 MS.gene004369:intron 35.0%
TCTTCGAGTATAGATACACA+AGG - chr2.4:67628195-67628214 MS.gene004369:CDS 35.0%
TCTTGAACAATTACTGGAAC+AGG + chr2.4:67626340-67626359 None:intergenic 35.0%
TTGATCAAACAGTTCATGTC+TGG + chr2.4:67626635-67626654 None:intergenic 35.0%
TTGATTAGGTATGTTGGAGT+TGG - chr2.4:67625128-67625147 MS.gene004369:CDS 35.0%
TTTGCGAACATTATCGTCAT+TGG + chr2.4:67627478-67627497 None:intergenic 35.0%
! CTCCAATCTTCTATTTTCAC+AGG - chr2.4:67627136-67627155 MS.gene004369:intron 35.0%
! GGATAACTCTAAAAGTGGAA+AGG + chr2.4:67627432-67627451 None:intergenic 35.0%
! TCACAAATTAAATTGGTGGC+AGG - chr2.4:67626440-67626459 MS.gene004369:intron 35.0%
! TTCCTTTTGTGCTTGAAACT+GGG - chr2.4:67625017-67625036 MS.gene004369:intron 35.0%
!! ACATTTCAAGTTTCAGTCCT+AGG + chr2.4:67625782-67625801 None:intergenic 35.0%
!! ATGAAAAAGGTGTCAAACCA+TGG - chr2.4:67626465-67626484 MS.gene004369:intron 35.0%
!! GATTGATCAAATTTTCAGGG+TGG - chr2.4:67628369-67628388 MS.gene004369:CDS 35.0%
!! GTTTTGACATGTTTAGTAGG+TGG - chr2.4:67628427-67628446 MS.gene004369:CDS 35.0%
!! TCCATTCCATATTTTAGACC+CGG - chr2.4:67627175-67627194 MS.gene004369:intron 35.0%
!! TCTGGTCTTTTGCTTGAAAT+TGG - chr2.4:67625260-67625279 MS.gene004369:intron 35.0%
ACTTATGCTACAACAGAGTC+TGG - chr2.4:67626057-67626076 MS.gene004369:CDS 40.0%
AGCTCTTCAATTTCTCAGGA+AGG - chr2.4:67626113-67626132 MS.gene004369:CDS 40.0%
AGGATGATCCTCTCATACTA+TGG - chr2.4:67625207-67625226 MS.gene004369:intron 40.0%
AGTATGAGAGGATCATCCTT+TGG + chr2.4:67625206-67625225 None:intergenic 40.0%
ATTACTCGACAGCTTAAGTG+GGG + chr2.4:67627293-67627312 None:intergenic 40.0%
CAAACCATGGATAAATCTCC+AGG - chr2.4:67626478-67626497 MS.gene004369:intron 40.0%
CACATTCATGGACAAAGGTT+AGG - chr2.4:67625867-67625886 MS.gene004369:CDS 40.0%
CATACAAATGACCAAGTTGC+AGG - chr2.4:67628032-67628051 MS.gene004369:intron 40.0%
CATTACTCGACAGCTTAAGT+GGG + chr2.4:67627294-67627313 None:intergenic 40.0%
CATTGGTCCAAAATCCCATA+AGG + chr2.4:67627461-67627480 None:intergenic 40.0%
CTAACCTTTGTCCATGAATG+TGG + chr2.4:67625869-67625888 None:intergenic 40.0%
CTGAATAAGTGTGTGAAGCT+CGG - chr2.4:67625548-67625567 MS.gene004369:CDS 40.0%
CTGTCTCCAATCATCAACAA+TGG + chr2.4:67628009-67628028 None:intergenic 40.0%
CTTACCTGGAGATTTATCCA+TGG + chr2.4:67626485-67626504 None:intergenic 40.0%
TACACAAGGACTTACTCCAA+TGG - chr2.4:67628209-67628228 MS.gene004369:intron 40.0%
TATGTGAATACCCTGCAACT+TGG + chr2.4:67628046-67628065 None:intergenic 40.0%
TCAGGGACCAAAATGATTGT+TGG - chr2.4:67625521-67625540 MS.gene004369:CDS 40.0%
TCCTGAGAAATTGAAGAGCT+CGG + chr2.4:67626113-67626132 None:intergenic 40.0%
TCTCCAGGTAAGCATCTTAA+AGG - chr2.4:67626493-67626512 MS.gene004369:intron 40.0%
TCTTCAAGGACTTACTCCAA+TGG - chr2.4:67628143-67628162 MS.gene004369:CDS 40.0%
TGAATCAGAGAGGAATCCAA+AGG - chr2.4:67625187-67625206 MS.gene004369:intron 40.0%
TGCTTAATGAGAGATCTCCT+TGG + chr2.4:67627233-67627252 None:intergenic 40.0%
TGGCAAGTAACAAGATACCT+AGG - chr2.4:67625762-67625781 MS.gene004369:intron 40.0%
TGTAGCATAAGTAAAGCCTG+TGG + chr2.4:67626049-67626068 None:intergenic 40.0%
TGTCAAAACATTCCTTAGGC+CGG + chr2.4:67628416-67628435 None:intergenic 40.0%
TTAGGGATATCCCTCTTATG+AGG + chr2.4:67627767-67627786 None:intergenic 40.0%
! ATCCCTTTTGCTCATGGAAT+GGG - chr2.4:67626803-67626822 MS.gene004369:intron 40.0%
! CTATTTTCACAGGCTACATC+TGG - chr2.4:67627146-67627165 MS.gene004369:intron 40.0%
! CTTCCTTTTGTGCTTGAAAC+TGG - chr2.4:67625016-67625035 MS.gene004369:intron 40.0%
! TACAGTCCGGGTCTAAAATA+TGG + chr2.4:67627184-67627203 None:intergenic 40.0%
! TATGTGATCCCTTTTGCTCA+TGG - chr2.4:67626797-67626816 MS.gene004369:intron 40.0%
! TCCGGGTCTAAAATATGGAA+TGG + chr2.4:67627179-67627198 None:intergenic 40.0%
! TTGGTGGCAGGAAATGAAAA+AGG - chr2.4:67626452-67626471 MS.gene004369:intron 40.0%
!! ATGTTGGAGTTGGTGAGAAA+GGG - chr2.4:67625138-67625157 MS.gene004369:intron 40.0%
!! ATTGGTGGTGATTCATACTC+TGG - chr2.4:67626310-67626329 MS.gene004369:CDS 40.0%
!! GATCCTTTAAGATGCTTACC+TGG + chr2.4:67626499-67626518 None:intergenic 40.0%
!! TATGTTGGAGTTGGTGAGAA+AGG - chr2.4:67625137-67625156 MS.gene004369:intron 40.0%
!! TGATCAAATTTTCAGGGTGG+AGG - chr2.4:67628372-67628391 MS.gene004369:CDS 40.0%
!! TTGTTGATGATTGGAGACAG+TGG - chr2.4:67628009-67628028 MS.gene004369:intron 40.0%
!! TTTCTTAAGGGGTACCTGTT+GGG - chr2.4:67626743-67626762 MS.gene004369:intron 40.0%
!!! TTTTCTTAAGGGGTACCTGT+TGG - chr2.4:67626742-67626761 MS.gene004369:intron 40.0%
ACATGGCAATGCCATTCTTG+GGG - chr2.4:67627952-67627971 MS.gene004369:intron 45.0%
ACCGAGCTCTTCAATTTCTC+AGG - chr2.4:67626109-67626128 MS.gene004369:CDS 45.0%
AGTCACGACAACTCACATTC+AGG - chr2.4:67625503-67625522 MS.gene004369:intron 45.0%
AGTCCCATTCCATGAGCAAA+AGG + chr2.4:67626809-67626828 None:intergenic 45.0%
GAATGCTGCAACAACTGGAA+AGG - chr2.4:67626766-67626785 MS.gene004369:intron 45.0%
GACATGGCAATGCCATTCTT+GGG - chr2.4:67627951-67627970 MS.gene004369:intron 45.0%
GACCCAGTTTCAAGCACAAA+AGG + chr2.4:67625022-67625041 None:intergenic 45.0%
GCACAAAAGGAAGTGATCCT+TGG + chr2.4:67625009-67625028 None:intergenic 45.0%
GCATTACTCGACAGCTTAAG+TGG + chr2.4:67627295-67627314 None:intergenic 45.0%
GTCACGACAACTCACATTCA+GGG - chr2.4:67625504-67625523 MS.gene004369:intron 45.0%
GTCCCATTCCATGAGCAAAA+GGG + chr2.4:67626808-67626827 None:intergenic 45.0%
TAAGTAAAGCCTGTGGAAGC+AGG + chr2.4:67626042-67626061 None:intergenic 45.0%
TCAACAAGCCACATACAGTC+CGG + chr2.4:67627197-67627216 None:intergenic 45.0%
TCCATAGTACACTCTCTTCC+TGG - chr2.4:67624980-67624999 MS.gene004369:intron 45.0%
! GATCCCTTTTGCTCATGGAA+TGG - chr2.4:67626802-67626821 MS.gene004369:intron 45.0%
! TCCAGGAAGAGAGTGTACTA+TGG + chr2.4:67624984-67625003 None:intergenic 45.0%
! TTTTAGACCCGGACTGTATG+TGG - chr2.4:67627186-67627205 MS.gene004369:intron 45.0%
!! CAAAGCACTGCACATACGTA+AGG - chr2.4:67627494-67627513 MS.gene004369:CDS 45.0%
!! TCATGTCTGGTTCTCATGTC+TGG + chr2.4:67626622-67626641 None:intergenic 45.0%
!! TGTTGGAGTTGGTGAGAAAG+GGG - chr2.4:67625139-67625158 MS.gene004369:intron 45.0%
!! TTCTTAAGGGGTACCTGTTG+GGG - chr2.4:67626744-67626763 MS.gene004369:intron 45.0%
CAACAAGCCACATACAGTCC+GGG + chr2.4:67627196-67627215 None:intergenic 50.0%
CACTCTCTTCCTGGATTCCA+AGG - chr2.4:67624989-67625008 MS.gene004369:intron 50.0%
CATGCTTGTGTCCCCAAGAA+TGG + chr2.4:67627966-67627985 None:intergenic 50.0%
CATTCTTGGGGACACAAGCA+TGG - chr2.4:67627964-67627983 MS.gene004369:intron 50.0%
CCAGTGAGCCATAGTATGAG+AGG + chr2.4:67625218-67625237 None:intergenic 50.0%
CCTCTCATACTATGGCTCAC+TGG - chr2.4:67625215-67625234 MS.gene004369:intron 50.0%
CGACATGGCAATGCCATTCT+TGG - chr2.4:67627950-67627969 MS.gene004369:intron 50.0%
CTCATACTATGGCTCACTGG+TGG - chr2.4:67625218-67625237 MS.gene004369:intron 50.0%
GAGGCCACATTCATGGACAA+AGG - chr2.4:67625862-67625881 MS.gene004369:CDS 50.0%
GCTTGCCTAATCTGATCCTG+AGG - chr2.4:67625843-67625862 MS.gene004369:intron 50.0%
GGAAGTGATCCTTGGAATCC+AGG + chr2.4:67625001-67625020 None:intergenic 50.0%
TAACAGTGGCGATCACGACA+TGG - chr2.4:67627935-67627954 MS.gene004369:intron 50.0%
TGATCCTGAGGCCACATTCA+TGG - chr2.4:67625855-67625874 MS.gene004369:CDS 50.0%
TGTGGCCTCAGGATCAGATT+AGG + chr2.4:67625851-67625870 None:intergenic 50.0%
TTGGGGAATGCTGCAACAAC+TGG - chr2.4:67626761-67626780 MS.gene004369:intron 50.0%
TTGTCCATGAATGTGGCCTC+AGG + chr2.4:67625862-67625881 None:intergenic 50.0%
TTGTTGCAGCATTCCCCAAC+AGG + chr2.4:67626760-67626779 None:intergenic 50.0%
!! TGCAGGGAAGTGTTGCGAAA+TGG - chr2.4:67627720-67627739 MS.gene004369:intron 50.0%
AGCTTGGTTGCACGTGTTGC+AGG - chr2.4:67627703-67627722 MS.gene004369:intron 55.0%
CATTCCTTAGGCCGGTACTC+CGG + chr2.4:67628408-67628427 None:intergenic 55.0%
CCAGACAAAGCAGAGCAACC+AGG + chr2.4:67625245-67625264 None:intergenic 55.0%
CCTGGTTGCTCTGCTTTGTC+TGG - chr2.4:67625242-67625261 MS.gene004369:intron 55.0%
GCAGATTTGCCTGCTTCCAC+AGG - chr2.4:67626030-67626049 MS.gene004369:intron 55.0%
GCTTGGTTGCACGTGTTGCA+GGG - chr2.4:67627704-67627723 MS.gene004369:intron 55.0%
! AGACCTCGTGCAAGAGTGCA+CGG + chr2.4:67627651-67627670 None:intergenic 55.0%
! GCAAGAGTGCACGGATGTGA+AGG + chr2.4:67627642-67627661 None:intergenic 55.0%
CATCCGTGCACTCTTGCACG+AGG - chr2.4:67627645-67627664 MS.gene004369:intron 60.0%
GACACACAGCTCCGGAGTAC+CGG - chr2.4:67628394-67628413 MS.gene004369:CDS 60.0%
! CTATGGCTCACTGGTGGACC+TGG - chr2.4:67625224-67625243 MS.gene004369:intron 60.0%
GGGTGGAGGACACACAGCTC+CGG - chr2.4:67628386-67628405 MS.gene004369:CDS 65.0%
!! AGCTCCGGAGTACCGGCCTA+AGG - chr2.4:67628401-67628420 MS.gene004369:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 67624890 67628470 67624890 ID=MS.gene004369
chr2.4 mRNA 67624890 67628470 67624890 ID=MS.gene004369.t1;Parent=MS.gene004369
chr2.4 exon 67628322 67628470 67628322 ID=MS.gene004369.t1.exon1;Parent=MS.gene004369.t1
chr2.4 CDS 67628322 67628470 67628322 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
chr2.4 exon 67628079 67628224 67628079 ID=MS.gene004369.t1.exon2;Parent=MS.gene004369.t1
chr2.4 CDS 67628079 67628224 67628079 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
chr2.4 exon 67627477 67627553 67627477 ID=MS.gene004369.t1.exon3;Parent=MS.gene004369.t1
chr2.4 CDS 67627477 67627553 67627477 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
chr2.4 exon 67627226 67627348 67627226 ID=MS.gene004369.t1.exon4;Parent=MS.gene004369.t1
chr2.4 CDS 67627226 67627348 67627226 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
chr2.4 exon 67626990 67627092 67626990 ID=MS.gene004369.t1.exon5;Parent=MS.gene004369.t1
chr2.4 CDS 67626990 67627092 67626990 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
chr2.4 exon 67626861 67626898 67626861 ID=MS.gene004369.t1.exon6;Parent=MS.gene004369.t1
chr2.4 CDS 67626861 67626898 67626861 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
chr2.4 exon 67626510 67626608 67626510 ID=MS.gene004369.t1.exon7;Parent=MS.gene004369.t1
chr2.4 CDS 67626510 67626608 67626510 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
chr2.4 exon 67626290 67626376 67626290 ID=MS.gene004369.t1.exon8;Parent=MS.gene004369.t1
chr2.4 CDS 67626290 67626376 67626290 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
chr2.4 exon 67626053 67626202 67626053 ID=MS.gene004369.t1.exon9;Parent=MS.gene004369.t1
chr2.4 CDS 67626053 67626202 67626053 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
chr2.4 exon 67625845 67625916 67625845 ID=MS.gene004369.t1.exon10;Parent=MS.gene004369.t1
chr2.4 CDS 67625845 67625916 67625845 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
chr2.4 exon 67625517 67625635 67625517 ID=MS.gene004369.t1.exon11;Parent=MS.gene004369.t1
chr2.4 CDS 67625517 67625635 67625517 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
chr2.4 exon 67625306 67625419 67625306 ID=MS.gene004369.t1.exon12;Parent=MS.gene004369.t1
chr2.4 CDS 67625306 67625419 67625306 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
chr2.4 exon 67625107 67625152 67625107 ID=MS.gene004369.t1.exon13;Parent=MS.gene004369.t1
chr2.4 CDS 67625107 67625152 67625107 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
chr2.4 exon 67624890 67624973 67624890 ID=MS.gene004369.t1.exon14;Parent=MS.gene004369.t1
chr2.4 CDS 67624890 67624973 67624890 ID=cds.MS.gene004369.t1;Parent=MS.gene004369.t1
Gene Sequence

>MS.gene004369

ATGGCTAAGTTTAGTTCATGTTATGGAAAGGAACTTCTTTTTGTTCTTCTTTTATTATCACATTTTTCATTTCAACTTGCAAAATGTGGTTCCATAGTACACTCTCTTCCTGGATTCCAAGGATCACTTCCTTTTGTGCTTGAAACTGGGTCAGTTATACATTTAGCTATATAGTTTATGATTTTTAAGTTTCTTTAATCTATGTTATTGGTACTAAAATATTTCATATTCATATTTCTTGATTAGGTATGTTGGAGTTGGTGAGAAAGGGGATGTACAAGTTTTCTACTATTTCATTGAATCAGAGAGGAATCCAAAGGATGATCCTCTCATACTATGGCTCACTGGTGGACCTGGTTGCTCTGCTTTGTCTGGTCTTTTGCTTGAAATTGGTAATTGCAACTTTACTTTTGGATATTCTTACATTAAGCATTGAGCAAACAATCATTTGGTCTCGATTGGTAATTGCAACTTTAATTTATAGGCTAAATTGACCAATGAAAAAAATATTTGAGGACTAAATTGACTATCTAAAAACATATTCAATGACAAAATGACTAATAAAAAATATCTTTGTGGATGTATTTGCAATTTTTATACACTTGGTGACTATAGTCACGACAACTCACATTCAGGGACCAAAATGATTGTTGGTTTACTGAATAAGTGTGTGAAGCTCGGTTGATCCCTCGAAACATAAGCAAAGTTTAACTAAATAAATAAGTCTTTGAACACTTATAACATGTAAGTTGTGTAAAAAAAATAAAAAATGACATGTATACAAATTTGTTGACTTGATACTCTTTGAAATTACAGCCTACAGTTCAGATTGATATCTAAGATTTGAGTGTTCTTTTGTGATTTAGTCTACATGGCAAGTAACAAGATACCTAGGACTGAAACTTGAAATGTTGCAGGTCCACTTGAATTTAAAAAGGAGGAATACAATGGGAGCTTGCCTAATCTGATCCTGAGGCCACATTCATGGACAAAGGTTAGGGTTCTAGTCCACATATTATTGTTGTCCGAGTAAAAAATTTCGGCTTTATATTGTATATATTTATCAAATTCTTGTAAAGTATGTTCTTATTTACTGATATTTGTAATGATTTTTCTTGGTAGGTGAGTAACATTATATTTGCAGATTTGCCTGCTTCCACAGGCTTTACTTATGCTACAACAGAGTCTGGTGCTCAACGAAGCGACTCAATACTAGTTCACCGAGCTCTTCAATTTCTCAGGAAGGTATATTCACCCTAGTTTCTATTGCTTTCATTTCATAATGATCACTTTTTTCTTTGTTTTTAAATTTTGTTGCAGTTATATCAATCGGTCAATAAAATATAATGAAAACTAAGTTGTGAATAAATCTTTGTAGTGGTTGGTGGATCATCCAAAATTTCAATCAAATGAAGTTTACATTGGTGGTGATTCATACTCTGGCATTCCTGTTCCAGTAATTGTTCAAGAAATTATACAAGGTACAAAACTCATGGCTTGATTATGTATGTAAGAACGATTATGTTGCAATCTTAATTAAGTTTAATTATTCACAAATTAAATTGGTGGCAGGAAATGAAAAAGGTGTCAAACCATGGATAAATCTCCAGGTAAGCATCTTAAAGGATCATTCTCTCATGTTAGGAATGTTCGTTGAACTAGCTCTTTAAATTACAAATATACATTGTATGTTTTTATAATTTGAAAAATGCAAATTAAGTATTGAAAACCAGACATGAGAACCAGACATGAACTGTTTGATCAACTTGATGTCTTGATCATACAGACTTGCATGATATGAAACAAAAAAGTTTACTATAATAGTACTGCTTTGATTAATCATGAAATGTTGTTTTCTTAAGGGGTACCTGTTGGGGAATGCTGCAACAACTGGAAAGGAAAAAAACTATGTGATCCCTTTTGCTCATGGAATGGGACTCATATCCGATGAACTATACGACGTAATTGAAATGTAATTTCTCAGTTGCTAATTGAATTATAATTCTTCTGTATATATTTAGCTATTCTTATATTCATAACCTATGAAGCATCAATAAAATATATGCTAGCAAACTAATCTCTAATATTTTGCAGTCCTTGCAAAAAAATTGTAATGGAGACTATGTAAATGTAGATAAGAGAAATGTTTTATGTTCAAGAGATATGAGTTCATTTGATGAGGTATATATTTGCATCCATTTATTTTATCGAATTTTTTGAGAAGAATTTATATTCACAAAAGTGAACTCCAATCTTCTATTTTCACAGGCTACATCTGGACTTAATCCATTCCATATTTTAGACCCGGACTGTATGTGGCTTGTTGAAACTGAAAAATTTCCAAGGAGATCTCTCATTAAGCAATATCCCTCCATATTCCTCAATACAAATCTGAAATTGCCCCACTTAAGCTGTCGAGTAATGCTTCTTTTCCGTATATTTATGCAATGCAATGATAATCTTACTATATATACCATTTGCTGCATCATCGTATATATGTATAAATCTCGGAACATTTCTGTTAAATTGATGTCTGTATCCTTTCCACTTTTAGAGTTATCCAAATTTCCTTATGGGATTTTGGACCAATGACGATAATGTTCGCAAAGCACTGCACATACGTAAGGTATATAGTTTCATATATTCCAAACTTATATTGTTCTTATGTTGTGTATCCATTTATTCTTAGAAGCTTCTTCTTCTAATAACAAGTGTAAGAATTTCACACTTCTGCTTTTCATAATTATTTCCTTCACATCCGTGCACTCTTGCACGAGGTCTTTACTATTTAGTTATACAACTATATATGTAAGAGCTTGGTTGCACGTGTTGCAGGGAAGTGTTGCGAAATGGCATCGTTGTAGCTTTAATATACCTCATAAGAGGGATATCCCTAACAGCTATGATTATCATGTAAATCTCAGTAGAAAGGGCATTCGTTCACTAATTTACAGGTAAATAATCTTTCTCTATATTGCATAAGATAGCACTTATGAGAACTAGTTTCATAATTTCATACAGAAAATAGATTTTATGTATTTCTAATAACAGTGGCGATCACGACATGGCAATGCCATTCTTGGGGACACAAGCATGGATAAGATCTTTGAACTATTCCATTGTTGATGATTGGAGACAGTGGCATACAAATGACCAAGTTGCAGGGTATTCACATACCATAAATTTAGTTACTGTAATTATATTATGTATAATATAATAATAATCATGTTGTATTTATGACCAAGTTGAATATTCTTCAAGGACTTACTCCAATGGAATGACATTTGCAATATTTGTTTTAATATTCTTCGAGTATAGATACACAAGGACTTACTCCAATGGAATGACATTTGCAACTGTGAAGGCAAGCATCATGTTCCTCAATAGAAATAGATCATACATAATGCCTTTATGTTATAGGAATGTGTAATAGAGTCCTTTAGATATAGAATCATTACTAGTTTCTTTACTCTATTATTGATTGATCAAATTTTCAGGGTGGAGGACACACAGCTCCGGAGTACCGGCCTAAGGAATGTTTTGACATGTTTAGTAGGTGGATATCTAAGAGAGCTTTGTAG
Protein sequence

>MS.gene004369.t1

MAKFSSCYGKELLFVLLLLSHFSFQLAKCGSIVHSLPGFQGSLPFVLETGYVGVGEKGDVQVFYYFIESERNPKDDPLILWLTGGPGCSALSGLLLEIGPLEFKKEEYNGSLPNLILRPHSWTKVSNIIFADLPASTGFTYATTESGAQRSDSILVHRALQFLRKWLVDHPKFQSNEVYIGGDSYSGIPVPVIVQEIIQGNEKGVKPWINLQGYLLGNAATTGKEKNYVIPFAHGMGLISDELYDSLQKNCNGDYVNVDKRNVLCSRDMSSFDEATSGLNPFHILDPDCMWLVETEKFPRRSLIKQYPSIFLNTNLKLPHLSCRSYPNFLMGFWTNDDNVRKALHIRKGSVAKWHRCSFNIPHKRDIPNSYDYHVNLSRKGIRSLIYSGDHDMAMPFLGTQAWIRSLNYSIVDDWRQWHTNDQVAGYTRTYSNGMTFATVKGGGHTAPEYRPKECFDMFSRWISKRAL