Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94662.t1 | XP_024640815.1 | 62.6 | 334 | 67 | 8 | 1 | 290 | 1 | 320 | 2.90E-49 | 206.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94662.t1 | G7K4I5 | 58.2 | 359 | 69 | 9 | 1 | 290 | 1 | 347 | 3.5e-44 | 188.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene94662 | 0.833286 | 5.82E-56 | -1.69E-46 |
| MS.gene050807 | MS.gene94662 | 0.803955 | 2.72E-49 | -1.69E-46 |
| MS.gene050959 | MS.gene94662 | 0.80459 | 2.01E-49 | -1.69E-46 |
| MS.gene051626 | MS.gene94662 | 0.832899 | 7.26E-56 | -1.69E-46 |
| MS.gene051663 | MS.gene94662 | 0.803003 | 4.29E-49 | -1.69E-46 |
| MS.gene051789 | MS.gene94662 | 0.825985 | 3.48E-54 | -1.69E-46 |
| MS.gene052207 | MS.gene94662 | 0.813218 | 2.86E-51 | -1.69E-46 |
| MS.gene053074 | MS.gene94662 | 0.821094 | 4.85E-53 | -1.69E-46 |
| MS.gene053630 | MS.gene94662 | 0.815383 | 9.51E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene94662 | 0.814293 | 1.66E-51 | -1.69E-46 |
| MS.gene054184 | MS.gene94662 | 0.813468 | 2.52E-51 | -1.69E-46 |
| MS.gene054185 | MS.gene94662 | 0.818545 | 1.85E-52 | -1.69E-46 |
| MS.gene054473 | MS.gene94662 | 0.819557 | 1.09E-52 | -1.69E-46 |
| MS.gene056069 | MS.gene94662 | -0.83037 | 3.05E-55 | -1.69E-46 |
| MS.gene056875 | MS.gene94662 | 0.804096 | 2.54E-49 | -1.69E-46 |
| MS.gene057260 | MS.gene94662 | 0.80953 | 1.81E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene94662 | 0.820654 | 6.12E-53 | -1.69E-46 |
| MS.gene058749 | MS.gene94662 | 0.804739 | 1.87E-49 | -1.69E-46 |
| MS.gene059124 | MS.gene94662 | 0.810245 | 1.27E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene94662 | 0.801969 | 7.01E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene94662 | 0.84052 | 8.28E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94662.t1 | MTR_5g094950 | 57.103 | 359 | 73 | 8 | 1 | 290 | 1 | 347 | 1.22e-105 | 322 |
| MS.gene94662.t1 | MTR_5g094970 | 51.104 | 317 | 69 | 8 | 70 | 316 | 6 | 306 | 1.97e-75 | 237 |
| MS.gene94662.t1 | MTR_5g095050 | 36.945 | 563 | 160 | 17 | 1 | 372 | 1 | 559 | 1.34e-64 | 215 |
| MS.gene94662.t1 | MTR_5g094960 | 40.854 | 328 | 107 | 9 | 1 | 290 | 1 | 279 | 4.87e-52 | 180 |
| MS.gene94662.t1 | MTR_5g094850 | 40.058 | 342 | 132 | 9 | 3 | 309 | 1 | 304 | 1.33e-49 | 174 |
| MS.gene94662.t1 | MTR_5g094850 | 40.058 | 342 | 132 | 9 | 3 | 309 | 1 | 304 | 2.26e-49 | 174 |
| MS.gene94662.t1 | MTR_5g094920 | 34.596 | 396 | 107 | 9 | 1 | 290 | 1 | 350 | 1.55e-38 | 145 |
| MS.gene94662.t1 | MTR_5g094990 | 40.705 | 312 | 124 | 13 | 1 | 290 | 1 | 273 | 1.52e-36 | 138 |
| MS.gene94662.t1 | MTR_5g095370 | 41.206 | 199 | 70 | 5 | 1 | 196 | 1 | 155 | 8.24e-32 | 120 |
| MS.gene94662.t1 | MTR_5g094910 | 34.731 | 334 | 104 | 11 | 1 | 301 | 1 | 253 | 3.04e-28 | 114 |
| MS.gene94662.t1 | MTR_5g095400 | 46.341 | 164 | 56 | 3 | 26 | 185 | 1 | 136 | 1.10e-27 | 114 |
| MS.gene94662.t1 | MTR_5g095430 | 45.806 | 155 | 52 | 3 | 35 | 185 | 1 | 127 | 9.89e-27 | 111 |
| MS.gene94662.t1 | MTR_5g077500 | 45.122 | 164 | 59 | 4 | 178 | 311 | 97 | 259 | 1.67e-24 | 102 |
| MS.gene94662.t1 | MTR_5g095390 | 44.086 | 186 | 67 | 5 | 30 | 202 | 20 | 181 | 2.64e-21 | 94.4 |
| MS.gene94662.t1 | MTR_5g095310 | 39.456 | 147 | 61 | 3 | 35 | 179 | 1 | 121 | 1.66e-19 | 89.4 |
| MS.gene94662.t1 | MTR_5g095360 | 41.121 | 107 | 52 | 3 | 61 | 158 | 1 | 105 | 1.61e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGACCCATGTTCGATTT+TGG | 0.133002 | 5.3:-75918780 | None:intergenic |
| CTCAAAACCATTACATGTTA+AGG | 0.367230 | 5.3:+75919795 | MS.gene94662:CDS |
| TGAGTTAGATAATGAAGTTT+TGG | 0.377487 | 5.3:+75919339 | MS.gene94662:CDS |
| GTCATTTCTTCTAAGTTATG+AGG | 0.381921 | 5.3:-75918851 | None:intergenic |
| CTTACAACACAACGTTGAAA+TGG | 0.390778 | 5.3:+75918985 | MS.gene94662:CDS |
| TTGACATCCTTAACATGTAA+TGG | 0.392334 | 5.3:-75919802 | None:intergenic |
| GGGCTGCTTGCATCTTTGCC+TGG | 0.393057 | 5.3:-75919886 | None:intergenic |
| GAGAGGAACTTTGTCAGAAA+GGG | 0.410933 | 5.3:+75919050 | MS.gene94662:CDS |
| TTAGCTGATAGAGTCACTAT+TGG | 0.415973 | 5.3:+75919208 | MS.gene94662:CDS |
| TGAGCTAGAAGCTAAAGTTT+TGG | 0.417553 | 5.3:+75919426 | MS.gene94662:CDS |
| CCAATCTTCGGGTCACTCTC+TGG | 0.419534 | 5.3:-75919956 | None:intergenic |
| AAGAAAGAAGTACGTTGAGT+TGG | 0.438659 | 5.3:+75919237 | MS.gene94662:CDS |
| GGAGGATGTTGAAGCAAGTC+AGG | 0.455376 | 5.3:+75919630 | MS.gene94662:CDS |
| CGAGCTTCGAAGTAAATTGA+AGG | 0.461338 | 5.3:+75919498 | MS.gene94662:CDS |
| GACACATGAGGTGTTGCAAC+CGG | 0.466770 | 5.3:-75919673 | None:intergenic |
| AGAGAGGAACTTTGTCAGAA+AGG | 0.484616 | 5.3:+75919049 | MS.gene94662:CDS |
| ATGTCAAGAAAGCCAAAGTC+AGG | 0.490710 | 5.3:+75919818 | MS.gene94662:CDS |
| GAAGATGGTGATGAGGTTGA+TGG | 0.497731 | 5.3:+75919919 | MS.gene94662:CDS |
| TCTAACACTAGACCTGACTT+TGG | 0.506997 | 5.3:-75919830 | None:intergenic |
| AGCAAGAATCACGTAGTTGG+TGG | 0.527719 | 5.3:-75918804 | None:intergenic |
| AACAAGATGATTGATGGTCC+AGG | 0.535173 | 5.3:+75919868 | MS.gene94662:CDS |
| GAGGTTGATGGTATTGATGA+TGG | 0.540674 | 5.3:+75919931 | MS.gene94662:CDS |
| CTTATTCGAGTCTTTGAACA+CGG | 0.544179 | 5.3:+75919573 | MS.gene94662:CDS |
| TTCAGCAAGAATCACGTAGT+TGG | 0.558811 | 5.3:-75918807 | None:intergenic |
| GCATGAAAGAAATGCAAGGT+TGG | 0.577609 | 5.3:+75919609 | MS.gene94662:CDS |
| TGAGTTGAGGAAACTGTTTG+AGG | 0.587379 | 5.3:+75919168 | MS.gene94662:CDS |
| TGAAGTTGTAACAGATATGA+TGG | 0.587982 | 5.3:+75918826 | MS.gene94662:CDS |
| TCACCATCTTCAATCACAAG+TGG | 0.589505 | 5.3:-75919907 | None:intergenic |
| AGCCCACTTGTGATTGAAGA+TGG | 0.597478 | 5.3:+75919904 | MS.gene94662:CDS |
| GAAAGAGTTTCAGAAAAGAG+AGG | 0.598100 | 5.3:+75919033 | MS.gene94662:CDS |
| TACTATAAATGAGAAGCGTA+AGG | 0.599833 | 5.3:+75919543 | MS.gene94662:CDS |
| ATTGAACCCAAAATCGAACA+TGG | 0.604289 | 5.3:+75918773 | MS.gene94662:CDS |
| GTACGTTGAGTTGGAGTGTG+AGG | 0.619740 | 5.3:+75919246 | MS.gene94662:CDS |
| TGATCATGTTGCAAATGCAC+CGG | 0.620646 | 5.3:+75919654 | MS.gene94662:CDS |
| GCAACACCTCATGTGTCTGA+TGG | 0.630963 | 5.3:+75919679 | MS.gene94662:CDS |
| GAGAGAGGAACATTGAGTTG+AGG | 0.631931 | 5.3:+75919155 | MS.gene94662:CDS |
| TGAAAGAAATGCAAGGTTGG+AGG | 0.632876 | 5.3:+75919612 | MS.gene94662:CDS |
| TCTTTGAACACGGAGTTCAG+AGG | 0.634013 | 5.3:+75919583 | MS.gene94662:CDS |
| TTGAAATGGAGAAACTTGCA+AGG | 0.634595 | 5.3:+75918999 | MS.gene94662:CDS |
| CACCATCTTCAATCACAAGT+GGG | 0.636322 | 5.3:-75919906 | None:intergenic |
| CTGTTGTAGTACTAAGAGAG+AGG | 0.656292 | 5.3:+75919140 | MS.gene94662:CDS |
| TGTGATTGAAGATGGTGATG+AGG | 0.677350 | 5.3:+75919912 | MS.gene94662:CDS |
| GATTGCATGAAAGAAATGCA+AGG | 0.680697 | 5.3:+75919605 | MS.gene94662:CDS |
| TTGAACCCAAAATCGAACAT+GGG | 0.683973 | 5.3:+75918774 | MS.gene94662:CDS |
| TTTGTACCATCAGACACATG+AGG | 0.707522 | 5.3:-75919685 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGTTTAAGTTATATTTTAC+AGG | + | chr5.3:75919759-75919778 | MS.gene94662:intron | 15.0% |
| ! | TTGAAAAACAAGATGATTGA+TGG | + | chr5.3:75919862-75919881 | MS.gene94662:CDS | 25.0% |
| !! | GAAGTACTAAGAAAAAAGAA+TGG | + | chr5.3:75919316-75919335 | MS.gene94662:CDS | 25.0% |
| !! | TGAGTTAGATAATGAAGTTT+TGG | + | chr5.3:75919339-75919358 | MS.gene94662:CDS | 25.0% |
| CTCAAAACCATTACATGTTA+AGG | + | chr5.3:75919795-75919814 | MS.gene94662:CDS | 30.0% | |
| GAAGTTGAAAGAAAAATGTG+TGG | + | chr5.3:75919273-75919292 | MS.gene94662:CDS | 30.0% | |
| GTCATTTCTTCTAAGTTATG+AGG | - | chr5.3:75918854-75918873 | None:intergenic | 30.0% | |
| TGAAGTTGTAACAGATATGA+TGG | + | chr5.3:75918826-75918845 | MS.gene94662:CDS | 30.0% | |
| TTGACATCCTTAACATGTAA+TGG | - | chr5.3:75919805-75919824 | None:intergenic | 30.0% | |
| ! | TACTATAAATGAGAAGCGTA+AGG | + | chr5.3:75919543-75919562 | MS.gene94662:CDS | 30.0% |
| ! | TGGAGATTTTGAATGAGAAA+TGG | + | chr5.3:75919452-75919471 | MS.gene94662:CDS | 30.0% |
| ! | TTTGGAGTTGAAGCAAAAAA+GGG | + | chr5.3:75919357-75919376 | MS.gene94662:CDS | 30.0% |
| !!! | TTTTGGAGTTGAAGCAAAAA+AGG | + | chr5.3:75919356-75919375 | MS.gene94662:CDS | 30.0% |
| AAAAATGTGTGGAGGATGAT+AGG | + | chr5.3:75919284-75919303 | MS.gene94662:CDS | 35.0% | |
| ATTGAACCCAAAATCGAACA+TGG | + | chr5.3:75918773-75918792 | MS.gene94662:CDS | 35.0% | |
| CTTACAACACAACGTTGAAA+TGG | + | chr5.3:75918985-75919004 | MS.gene94662:CDS | 35.0% | |
| CTTATTCGAGTCTTTGAACA+CGG | + | chr5.3:75919573-75919592 | MS.gene94662:CDS | 35.0% | |
| GAAAGAGTTTCAGAAAAGAG+AGG | + | chr5.3:75919033-75919052 | MS.gene94662:CDS | 35.0% | |
| GATTGCATGAAAGAAATGCA+AGG | + | chr5.3:75919605-75919624 | MS.gene94662:CDS | 35.0% | |
| GTTGAAAGAAAAATGTGTGG+AGG | + | chr5.3:75919276-75919295 | MS.gene94662:CDS | 35.0% | |
| TTAGCTGATAGAGTCACTAT+TGG | + | chr5.3:75919208-75919227 | MS.gene94662:CDS | 35.0% | |
| TTGAAATGGAGAAACTTGCA+AGG | + | chr5.3:75918999-75919018 | MS.gene94662:CDS | 35.0% | |
| TTGAACCCAAAATCGAACAT+GGG | + | chr5.3:75918774-75918793 | MS.gene94662:CDS | 35.0% | |
| ! | AAGAAAGAAGTACGTTGAGT+TGG | + | chr5.3:75919237-75919256 | MS.gene94662:CDS | 35.0% |
| ! | TGAGCTAGAAGCTAAAGTTT+TGG | + | chr5.3:75919426-75919445 | MS.gene94662:CDS | 35.0% |
| !!! | AGAAGCTAAAGTTTTGGAGT+TGG | + | chr5.3:75919432-75919451 | MS.gene94662:CDS | 35.0% |
| AGAGAGGAACTTTGTCAGAA+AGG | + | chr5.3:75919049-75919068 | MS.gene94662:CDS | 40.0% | |
| ATGTCAAGAAAGCCAAAGTC+AGG | + | chr5.3:75919818-75919837 | MS.gene94662:CDS | 40.0% | |
| CACCATCTTCAATCACAAGT+GGG | - | chr5.3:75919909-75919928 | None:intergenic | 40.0% | |
| CGAGCTTCGAAGTAAATTGA+AGG | + | chr5.3:75919498-75919517 | MS.gene94662:CDS | 40.0% | |
| CTGTTGTAGTACTAAGAGAG+AGG | + | chr5.3:75919140-75919159 | MS.gene94662:CDS | 40.0% | |
| GAGAGGAACTTTGTCAGAAA+GGG | + | chr5.3:75919050-75919069 | MS.gene94662:CDS | 40.0% | |
| GCATGAAAGAAATGCAAGGT+TGG | + | chr5.3:75919609-75919628 | MS.gene94662:CDS | 40.0% | |
| TCACCATCTTCAATCACAAG+TGG | - | chr5.3:75919910-75919929 | None:intergenic | 40.0% | |
| TCTAACACTAGACCTGACTT+TGG | - | chr5.3:75919833-75919852 | None:intergenic | 40.0% | |
| TGAAAGAAATGCAAGGTTGG+AGG | + | chr5.3:75919612-75919631 | MS.gene94662:CDS | 40.0% | |
| TGATCATGTTGCAAATGCAC+CGG | + | chr5.3:75919654-75919673 | MS.gene94662:CDS | 40.0% | |
| TTCAGCAAGAATCACGTAGT+TGG | - | chr5.3:75918810-75918829 | None:intergenic | 40.0% | |
| TTTGTACCATCAGACACATG+AGG | - | chr5.3:75919688-75919707 | None:intergenic | 40.0% | |
| ! | AACAAGATGATTGATGGTCC+AGG | + | chr5.3:75919868-75919887 | MS.gene94662:CDS | 40.0% |
| ! | AGATGACCCATGTTCGATTT+TGG | - | chr5.3:75918783-75918802 | None:intergenic | 40.0% |
| ! | GATGACCCATGTTCGATTTT+GGG | - | chr5.3:75918782-75918801 | None:intergenic | 40.0% |
| ! | TGAGTTGAGGAAACTGTTTG+AGG | + | chr5.3:75919168-75919187 | MS.gene94662:CDS | 40.0% |
| ! | TGTGATTGAAGATGGTGATG+AGG | + | chr5.3:75919912-75919931 | MS.gene94662:CDS | 40.0% |
| !! | GAGGTTGATGGTATTGATGA+TGG | + | chr5.3:75919931-75919950 | MS.gene94662:CDS | 40.0% |
| AAGCAAAAAAGGGTGGAGGA+TGG | + | chr5.3:75919367-75919386 | MS.gene94662:CDS | 45.0% | |
| AGCAAAAAAGGGTGGAGGAT+GGG | + | chr5.3:75919368-75919387 | MS.gene94662:CDS | 45.0% | |
| AGCAAGAATCACGTAGTTGG+TGG | - | chr5.3:75918807-75918826 | None:intergenic | 45.0% | |
| AGCCCACTTGTGATTGAAGA+TGG | + | chr5.3:75919904-75919923 | MS.gene94662:CDS | 45.0% | |
| GGAGTTGAAGCAAAAAAGGG+TGG | + | chr5.3:75919360-75919379 | MS.gene94662:CDS | 45.0% | |
| GTTGAAGCAAAAAAGGGTGG+AGG | + | chr5.3:75919363-75919382 | MS.gene94662:CDS | 45.0% | |
| TCTTTGAACACGGAGTTCAG+AGG | + | chr5.3:75919583-75919602 | MS.gene94662:CDS | 45.0% | |
| ! | GAAGATGGTGATGAGGTTGA+TGG | + | chr5.3:75919919-75919938 | MS.gene94662:CDS | 45.0% |
| ! | GAGAGAGGAACATTGAGTTG+AGG | + | chr5.3:75919155-75919174 | MS.gene94662:CDS | 45.0% |
| GCAACACCTCATGTGTCTGA+TGG | + | chr5.3:75919679-75919698 | MS.gene94662:CDS | 50.0% | |
| GGAGGATGTTGAAGCAAGTC+AGG | + | chr5.3:75919630-75919649 | MS.gene94662:CDS | 50.0% | |
| !! | GACACATGAGGTGTTGCAAC+CGG | - | chr5.3:75919676-75919695 | None:intergenic | 50.0% |
| !! | GTACGTTGAGTTGGAGTGTG+AGG | + | chr5.3:75919246-75919265 | MS.gene94662:CDS | 50.0% |
| GGGCTGCTTGCATCTTTGCC+TGG | - | chr5.3:75919889-75919908 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 75918767 | 75919966 | 75918767 | ID=MS.gene94662 |
| chr5.3 | mRNA | 75918767 | 75919966 | 75918767 | ID=MS.gene94662.t1;Parent=MS.gene94662 |
| chr5.3 | exon | 75918767 | 75919708 | 75918767 | ID=MS.gene94662.t1.exon1;Parent=MS.gene94662.t1 |
| chr5.3 | CDS | 75918767 | 75919708 | 75918767 | ID=cds.MS.gene94662.t1;Parent=MS.gene94662.t1 |
| chr5.3 | exon | 75919781 | 75919966 | 75919781 | ID=MS.gene94662.t1.exon2;Parent=MS.gene94662.t1 |
| chr5.3 | CDS | 75919781 | 75919966 | 75919781 | ID=cds.MS.gene94662.t1;Parent=MS.gene94662.t1 |
| Gene Sequence |
| Protein sequence |
