Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95454.t1 | GAU41975.1 | 37.8 | 74 | 46 | 0 | 13 | 86 | 396 | 469 | 2.90E-06 | 61.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95454.t1 | A0A2Z6P118 | 37.8 | 74 | 46 | 0 | 13 | 86 | 396 | 469 | 2.1e-06 | 61.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049910 | MS.gene95454 | 0.807773 | 4.29E-50 | -1.69E-46 |
| MS.gene050397 | MS.gene95454 | 0.820577 | 6.37E-53 | -1.69E-46 |
| MS.gene051983 | MS.gene95454 | 0.808874 | 2.50E-50 | -1.69E-46 |
| MS.gene052011 | MS.gene95454 | -0.810803 | 9.61E-51 | -1.69E-46 |
| MS.gene052077 | MS.gene95454 | 0.807834 | 4.16E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene95454 | 0.810538 | 1.10E-50 | -1.69E-46 |
| MS.gene052253 | MS.gene95454 | 0.809866 | 1.53E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene95454 | 0.816582 | 5.14E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene95454 | 0.81776 | 2.79E-52 | -1.69E-46 |
| MS.gene055277 | MS.gene95454 | 0.815266 | 1.01E-51 | -1.69E-46 |
| MS.gene05553 | MS.gene95454 | 0.805461 | 1.32E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene95454 | 0.80367 | 3.12E-49 | -1.69E-46 |
| MS.gene056659 | MS.gene95454 | 0.812108 | 5.00E-51 | -1.69E-46 |
| MS.gene056979 | MS.gene95454 | 0.826137 | 3.20E-54 | -1.69E-46 |
| MS.gene05811 | MS.gene95454 | 0.818215 | 2.20E-52 | -1.69E-46 |
| MS.gene05907 | MS.gene95454 | 0.817911 | 2.58E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene95454 | 0.803117 | 4.06E-49 | -1.69E-46 |
| MS.gene059692 | MS.gene95454 | 0.802765 | 4.80E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene95454 | 0.81612 | 6.51E-52 | -1.69E-46 |
| MS.gene059894 | MS.gene95454 | 0.827333 | 1.66E-54 | -1.69E-46 |
| MS.gene061049 | MS.gene95454 | 0.829473 | 5.05E-55 | -1.69E-46 |
| MS.gene061159 | MS.gene95454 | 0.807922 | 3.99E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGAGATATACGCTATTT+TGG | 0.235183 | 7.4:-80989843 | MS.gene95454:CDS |
| TAGACTGTTTCCGTTATCTA+TGG | 0.335130 | 7.4:-80989776 | MS.gene95454:CDS |
| TTAACAAGGTTGTCCTTTGC+TGG | 0.345200 | 7.4:+80989665 | None:intergenic |
| AGGTTAGAGAAGGGTGTTGA+GGG | 0.367493 | 7.4:-80989913 | MS.gene95454:CDS |
| TGTGTTTAGTCTTGGATGAT+TGG | 0.392419 | 7.4:-80989861 | MS.gene95454:CDS |
| CTTGGAAAACTATACAAAAT+CGG | 0.406653 | 7.4:-80989691 | MS.gene95454:CDS |
| AAGATCAGTGTGTTTAGTCT+TGG | 0.452847 | 7.4:-80989869 | MS.gene95454:CDS |
| TATCGTTTGGAAGTGATCAA+TGG | 0.466251 | 7.4:-80990230 | MS.gene95454:CDS |
| TGGTCGAAACTGTGGATGAC+TGG | 0.470210 | 7.4:-80989823 | MS.gene95454:CDS |
| GAAGATAGAGAGGTTAGAGA+AGG | 0.477947 | 7.4:-80989923 | MS.gene95454:CDS |
| AAGATAGAGAGGTTAGAGAA+GGG | 0.481333 | 7.4:-80989922 | MS.gene95454:CDS |
| GTTAGAGAAGGGTGTTGAGG+GGG | 0.491613 | 7.4:-80989911 | MS.gene95454:CDS |
| TCATTTGAAGGTGTTTCGCT+TGG | 0.498744 | 7.4:-80989709 | MS.gene95454:CDS |
| GAGGTTAGAGAAGGGTGTTG+AGG | 0.499476 | 7.4:-80989914 | MS.gene95454:CDS |
| GCATGTGATCAGTCATTTGA+AGG | 0.504030 | 7.4:-80989721 | MS.gene95454:CDS |
| CAATTATTCCACGCTTAACA+AGG | 0.510894 | 7.4:+80989651 | None:intergenic |
| ACTTTGACGTCCATAGATAA+CGG | 0.516390 | 7.4:+80989766 | None:intergenic |
| GGTTAGAGAAGGGTGTTGAG+GGG | 0.543146 | 7.4:-80989912 | MS.gene95454:CDS |
| ACAAAATCGGTTACCAGCAA+AGG | 0.559696 | 7.4:-80989678 | MS.gene95454:CDS |
| GTTATCTATGGACGTCAAAG+TGG | 0.576530 | 7.4:-80989764 | MS.gene95454:CDS |
| GGTCGAAACTGTGGATGACT+GGG | 0.598690 | 7.4:-80989822 | MS.gene95454:CDS |
| AAGGACAACCTTGTTAAGCG+TGG | 0.624456 | 7.4:-80989659 | MS.gene95454:CDS |
| CTCTCTATCTTCTTCCACCA+CGG | 0.631721 | 7.4:+80989934 | None:intergenic |
| CGTGGTGGAAGAAGATAGAG+AGG | 0.671187 | 7.4:-80989933 | MS.gene95454:CDS |
| GGGTGTTTGATTCACCGTGG+TGG | 0.704104 | 7.4:-80989948 | MS.gene95454:CDS |
| GTTGGGTGTTTGATTCACCG+TGG | 0.716424 | 7.4:-80989951 | MS.gene95454:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCTATTTAAATCTAAGTAT+GGG | - | chr7.4:80989896-80989915 | MS.gene95454:CDS | 15.0% |
| !! | GATCTATTTAAATCTAAGTA+TGG | - | chr7.4:80989895-80989914 | MS.gene95454:CDS | 20.0% |
| !! | GGAAGACTATAAAAATTATT+TGG | - | chr7.4:80990151-80990170 | MS.gene95454:intron | 20.0% |
| ! | CTTGGAAAACTATACAAAAT+CGG | - | chr7.4:80990203-80990222 | MS.gene95454:intron | 25.0% |
| ! | TGATGATTATATTCTTGTAG+AGG | - | chr7.4:80989842-80989861 | MS.gene95454:CDS | 25.0% |
| ! | ATTGGAGATATACGCTATTT+TGG | - | chr7.4:80990051-80990070 | MS.gene95454:intron | 30.0% |
| AAACTGAGAAAAGTAGGTTG+TGG | - | chr7.4:80989869-80989888 | MS.gene95454:CDS | 35.0% | |
| AAGATAGAGAGGTTAGAGAA+GGG | - | chr7.4:80989972-80989991 | MS.gene95454:intron | 35.0% | |
| AAGATCAGTGTGTTTAGTCT+TGG | - | chr7.4:80990025-80990044 | MS.gene95454:intron | 35.0% | |
| ACTTTGACGTCCATAGATAA+CGG | + | chr7.4:80990131-80990150 | None:intergenic | 35.0% | |
| GGCTAAAAACTGAGAAAAGT+AGG | - | chr7.4:80989863-80989882 | MS.gene95454:CDS | 35.0% | |
| TAGACTGTTTCCGTTATCTA+TGG | - | chr7.4:80990118-80990137 | MS.gene95454:intron | 35.0% | |
| TATCGTTTGGAAGTGATCAA+TGG | - | chr7.4:80989664-80989683 | MS.gene95454:CDS | 35.0% | |
| !! | TGTGTTTAGTCTTGGATGAT+TGG | - | chr7.4:80990033-80990052 | MS.gene95454:intron | 35.0% |
| ACAAAATCGGTTACCAGCAA+AGG | - | chr7.4:80990216-80990235 | MS.gene95454:CDS | 40.0% | |
| ATCTAAGTATGGGCATGTTG+AGG | - | chr7.4:80989906-80989925 | MS.gene95454:CDS | 40.0% | |
| ATGTTGAGGGTTAAATCGGA+AGG | - | chr7.4:80989920-80989939 | MS.gene95454:CDS | 40.0% | |
| GAAGATAGAGAGGTTAGAGA+AGG | - | chr7.4:80989971-80989990 | MS.gene95454:intron | 40.0% | |
| GCATGTGATCAGTCATTTGA+AGG | - | chr7.4:80990173-80990192 | MS.gene95454:intron | 40.0% | |
| GTTATCTATGGACGTCAAAG+TGG | - | chr7.4:80990130-80990149 | MS.gene95454:intron | 40.0% | |
| TCTAAGTATGGGCATGTTGA+GGG | - | chr7.4:80989907-80989926 | MS.gene95454:CDS | 40.0% | |
| TGTTGAGGGTTAAATCGGAA+GGG | - | chr7.4:80989921-80989940 | MS.gene95454:CDS | 40.0% | |
| TTAACAAGGTTGTCCTTTGC+TGG | + | chr7.4:80990232-80990251 | None:intergenic | 40.0% | |
| TTAACATGACGAGAGTAGCA+TGG | - | chr7.4:80989734-80989753 | MS.gene95454:CDS | 40.0% | |
| !! | TCATTTGAAGGTGTTTCGCT+TGG | - | chr7.4:80990185-80990204 | MS.gene95454:intron | 40.0% |
| !! | TTTGCTCTGAAACTTGTGCT+TGG | - | chr7.4:80989771-80989790 | MS.gene95454:CDS | 40.0% |
| AAGGACAACCTTGTTAAGCG+TGG | - | chr7.4:80990235-80990254 | MS.gene95454:CDS | 45.0% | |
| AGGGTTAAATCGGAAGGGTT+GGG | - | chr7.4:80989926-80989945 | MS.gene95454:CDS | 45.0% | |
| CTCTCTATCTTCTTCCACCA+CGG | + | chr7.4:80989963-80989982 | None:intergenic | 45.0% | |
| GGGCATGTTGAGGGTTAAAT+CGG | - | chr7.4:80989916-80989935 | MS.gene95454:CDS | 45.0% | |
| !! | AGGTTAGAGAAGGGTGTTGA+GGG | - | chr7.4:80989981-80990000 | MS.gene95454:intron | 45.0% |
| !!! | CGCTATTTTGGTCGAAACTG+TGG | - | chr7.4:80990063-80990082 | MS.gene95454:intron | 45.0% |
| GAGGGTTAAATCGGAAGGGT+TGG | - | chr7.4:80989925-80989944 | MS.gene95454:CDS | 50.0% | |
| GGTCGAAACTGTGGATGACT+GGG | - | chr7.4:80990072-80990091 | MS.gene95454:intron | 50.0% | |
| GTTGGGTGTTTGATTCACCG+TGG | - | chr7.4:80989943-80989962 | MS.gene95454:CDS | 50.0% | |
| TGGTCGAAACTGTGGATGAC+TGG | - | chr7.4:80990071-80990090 | MS.gene95454:intron | 50.0% | |
| ! | CGTGGTGGAAGAAGATAGAG+AGG | - | chr7.4:80989961-80989980 | MS.gene95454:intron | 50.0% |
| !! | GAGGTTAGAGAAGGGTGTTG+AGG | - | chr7.4:80989980-80989999 | MS.gene95454:intron | 50.0% |
| !! | GGTTAGAGAAGGGTGTTGAG+GGG | - | chr7.4:80989982-80990001 | MS.gene95454:intron | 50.0% |
| !! | GTTAGAGAAGGGTGTTGAGG+GGG | - | chr7.4:80989983-80990002 | MS.gene95454:intron | 50.0% |
| GGGTGTTTGATTCACCGTGG+TGG | - | chr7.4:80989946-80989965 | MS.gene95454:CDS | 55.0% | |
| TTGAGGGGGCAAGTCAGGGT+TGG | - | chr7.4:80989997-80990016 | MS.gene95454:intron | 60.0% | |
| ! | GGTGTTGAGGGGGCAAGTCA+GGG | - | chr7.4:80989993-80990012 | MS.gene95454:intron | 60.0% |
| !! | GGGTGTTGAGGGGGCAAGTC+AGG | - | chr7.4:80989992-80990011 | MS.gene95454:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 80989654 | 80990262 | 80989654 | ID=MS.gene95454 |
| chr7.4 | mRNA | 80989654 | 80990262 | 80989654 | ID=MS.gene95454.t1;Parent=MS.gene95454 |
| chr7.4 | exon | 80990215 | 80990262 | 80990215 | ID=MS.gene95454.t1.exon1;Parent=MS.gene95454.t1 |
| chr7.4 | CDS | 80990215 | 80990262 | 80990215 | ID=cds.MS.gene95454.t1;Parent=MS.gene95454.t1 |
| chr7.4 | exon | 80989654 | 80989974 | 80989654 | ID=MS.gene95454.t1.exon2;Parent=MS.gene95454.t1 |
| chr7.4 | CDS | 80989654 | 80989974 | 80989654 | ID=cds.MS.gene95454.t1;Parent=MS.gene95454.t1 |
| Gene Sequence |
| Protein sequence |
