AlfalfaGEDB | MS.gene96692

M. sativa cultivar XinJiangDaYe / MS.gene96692

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96692.t1	XP_013469983.1	93.4	76	5	0	1	76	38	113	3.60E-31	143.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96692.t1	A0A072VQS7	93.4	76	5	0	1	76	38	113	2.6e-31	143.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049573	MS.gene96692	0.823981	1.03E-53	-1.69E-46
MS.gene050363	MS.gene96692	0.804529	2.07E-49	-1.69E-46
MS.gene050883	MS.gene96692	0.811774	5.92E-51	-1.69E-46
MS.gene05142	MS.gene96692	0.843416	1.42E-58	-1.69E-46
MS.gene051946	MS.gene96692	0.854851	9.35E-62	-1.69E-46
MS.gene053826	MS.gene96692	0.82012	8.11E-53	-1.69E-46
MS.gene055401	MS.gene96692	0.866228	3.32E-65	-1.69E-46
MS.gene056310	MS.gene96692	0.865053	7.80E-65	-1.69E-46
MS.gene056311	MS.gene96692	0.832613	8.56E-56	-1.69E-46
MS.gene056313	MS.gene96692	0.847808	9.15E-60	-1.69E-46
MS.gene056408	MS.gene96692	0.82366	1.23E-53	-1.69E-46
MS.gene057014	MS.gene96692	0.839653	1.39E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96692.t1	MTR_1g106215	93.421	76	5	0	1	76	38	113	3.71e-48	148

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGTAGCTACCACATGTTAT+TGG	0.297025	1.1:-76432836	None:intergenic
ATTTGGTAATGATGGAAGTT+TGG	0.326402	1.1:-76432731	MS.gene96692:CDS
TTGGAATCTGACTCATTAAC+AGG	0.333936	1.1:-76432817	MS.gene96692:CDS
GAGCACAGATTTGGTAATGA+TGG	0.406744	1.1:-76432739	MS.gene96692:CDS
AGCATAAGAAGCAGCAGCAC+TGG	0.414803	1.1:+76432663	None:intergenic
AGCTGTCACAATGATGCCTC+TGG	0.415269	1.1:+76432702	None:intergenic
GAGTTCTTAGAGCACAGATT+TGG	0.435020	1.1:-76432748	MS.gene96692:CDS
TGAAATTCTTTGAGGGTGGT+TGG	0.465990	1.1:+76432638	None:intergenic
TGCTCTAAGAACTCAGTTGT+GGG	0.467329	1.1:+76432757	None:intergenic
GAAATTCTTTGAGGGTGGTT+GGG	0.475402	1.1:+76432639	None:intergenic
ATGTTGTTGAAATTCTTTGA+GGG	0.507140	1.1:+76432631	None:intergenic
GCTGTCACAATGATGCCTCT+GGG	0.532008	1.1:+76432703	None:intergenic
GCAGCACTGGCAGTGGTCGG+AGG	0.539102	1.1:+76432676	None:intergenic
GTGCTCTAAGAACTCAGTTG+TGG	0.553458	1.1:+76432756	None:intergenic
GCAGCAGCACTGGCAGTGGT+CGG	0.562924	1.1:+76432673	None:intergenic
AATGTTGTTGAAATTCTTTG+AGG	0.567996	1.1:+76432630	None:intergenic
AGAAGCAGCAGCACTGGCAG+TGG	0.577609	1.1:+76432669	None:intergenic
TTGTTGAAATTCTTTGAGGG+TGG	0.597370	1.1:+76432634	None:intergenic
AGACCTGGTTCTTCTATGAG+AGG	0.610651	1.1:+76432792	None:intergenic
GGAAGTTTGGTTAGCCCCAG+AGG	0.621319	1.1:-76432718	MS.gene96692:CDS
CTGTCACAATGATGCCTCTG+GGG	0.650846	1.1:+76432704	None:intergenic
GATCCTCTCATAGAAGAACC+AGG	0.662887	1.1:-76432795	MS.gene96692:CDS
GGGTTGCTGAAAATCAGACC+TGG	0.666499	1.1:+76432777	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATGTTGTTGAAATTCTTTG+AGG	+	chr1.1:76432811-76432830	None:intergenic	25.0%
!	ATGTTGTTGAAATTCTTTGA+GGG	+	chr1.1:76432810-76432829	None:intergenic	25.0%
!!	ATTTGGTAATGATGGAAGTT+TGG	-	chr1.1:76432707-76432726	MS.gene96692:CDS	30.0%
	TTGGAATCTGACTCATTAAC+AGG	-	chr1.1:76432621-76432640	MS.gene96692:CDS	35.0%
!	TTGTTGAAATTCTTTGAGGG+TGG	+	chr1.1:76432807-76432826	None:intergenic	35.0%
	GAGTTCTTAGAGCACAGATT+TGG	-	chr1.1:76432690-76432709	MS.gene96692:CDS	40.0%
!	GAAATTCTTTGAGGGTGGTT+GGG	+	chr1.1:76432802-76432821	None:intergenic	40.0%
!	TGAAATTCTTTGAGGGTGGT+TGG	+	chr1.1:76432803-76432822	None:intergenic	40.0%
!!	GAGCACAGATTTGGTAATGA+TGG	-	chr1.1:76432699-76432718	MS.gene96692:CDS	40.0%
!!	TGCTCTAAGAACTCAGTTGT+GGG	+	chr1.1:76432684-76432703	None:intergenic	40.0%
	GATCCTCTCATAGAAGAACC+AGG	-	chr1.1:76432643-76432662	MS.gene96692:CDS	45.0%
!	AGACCTGGTTCTTCTATGAG+AGG	+	chr1.1:76432649-76432668	None:intergenic	45.0%
!!	GTGCTCTAAGAACTCAGTTG+TGG	+	chr1.1:76432685-76432704	None:intergenic	45.0%
	AGCATAAGAAGCAGCAGCAC+TGG	+	chr1.1:76432778-76432797	None:intergenic	50.0%
	AGCTGTCACAATGATGCCTC+TGG	+	chr1.1:76432739-76432758	None:intergenic	50.0%
	CTGTCACAATGATGCCTCTG+GGG	+	chr1.1:76432737-76432756	None:intergenic	50.0%
	GCTGTCACAATGATGCCTCT+GGG	+	chr1.1:76432738-76432757	None:intergenic	50.0%
	GGGTTGCTGAAAATCAGACC+TGG	+	chr1.1:76432664-76432683	None:intergenic	50.0%
!	GGAAGTTTGGTTAGCCCCAG+AGG	-	chr1.1:76432720-76432739	MS.gene96692:CDS	55.0%
!!	AGAAGCAGCAGCACTGGCAG+TGG	+	chr1.1:76432772-76432791	None:intergenic	60.0%
!!	GCAGCAGCACTGGCAGTGGT+CGG	+	chr1.1:76432768-76432787	None:intergenic	65.0%
!!	GCAGCACTGGCAGTGGTCGG+AGG	+	chr1.1:76432765-76432784	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	76432615	76432845	76432615	ID=MS.gene96692
chr1.1	mRNA	76432615	76432845	76432615	ID=MS.gene96692.t1;Parent=MS.gene96692
chr1.1	exon	76432615	76432845	76432615	ID=MS.gene96692.t1.exon1;Parent=MS.gene96692.t1
chr1.1	CDS	76432615	76432845	76432615	ID=cds.MS.gene96692.t1;Parent=MS.gene96692.t1

Gene Sequence	>MS.gene96692 ATGTTATTGGAATCTGACTCATTAACAGGATCCTCTCATAGAAGAACCAGGTCTGATTTTCAGCAACCCACAACTGAGTTCTTAGAGCACAGATTTGGTAATGATGGAAGTTTGGTTAGCCCCAGAGGCATCATTGTGACAGCTCTGCCTCCGACCACTGCCAGTGCTGCTGCTTCTTATGCTACCCAACCACCCTCAAAGAATTTCAACAACATTCGCAAACACAAATGA
Protein sequence	>MS.gene96692.t1 MLLESDSLTGSSHRRTRSDFQQPTTEFLEHRFGNDGSLVSPRGIIVTALPPTTASAAASYATQPPSKNFNNIRKHK