Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97478.t1 | XP_013448631.1 | 100 | 184 | 0 | 0 | 1 | 184 | 32 | 215 | 6.80E-100 | 373.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97478.t1 | Q9ZTN2 | 88.6 | 184 | 21 | 0 | 1 | 184 | 32 | 215 | 3.1e-93 | 342.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97478.t1 | I3SA95 | 100.0 | 184 | 0 | 0 | 1 | 184 | 32 | 215 | 4.9e-100 | 373.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050330 | MS.gene97478 | 0.807619 | 4.63E-50 | -1.69E-46 |
| MS.gene050623 | MS.gene97478 | 0.807273 | 5.48E-50 | -1.69E-46 |
| MS.gene050680 | MS.gene97478 | -0.813972 | 1.95E-51 | -1.69E-46 |
| MS.gene050750 | MS.gene97478 | -0.823394 | 1.42E-53 | -1.69E-46 |
| MS.gene050905 | MS.gene97478 | 0.809492 | 1.84E-50 | -1.69E-46 |
| MS.gene050953 | MS.gene97478 | -0.830677 | 2.57E-55 | -1.69E-46 |
| MS.gene05119 | MS.gene97478 | 0.800105 | 1.68E-48 | -1.69E-46 |
| MS.gene051290 | MS.gene97478 | 0.802395 | 5.73E-49 | -1.69E-46 |
| MS.gene051926 | MS.gene97478 | 0.809117 | 2.22E-50 | -1.69E-46 |
| MS.gene051983 | MS.gene97478 | -0.815812 | 7.63E-52 | -1.69E-46 |
| MS.gene052309 | MS.gene97478 | 0.87559 | 2.73E-68 | -1.69E-46 |
| MS.gene052358 | MS.gene97478 | 0.812723 | 3.67E-51 | -1.69E-46 |
| MS.gene052965 | MS.gene97478 | 0.840786 | 7.05E-58 | -1.69E-46 |
| MS.gene05415 | MS.gene97478 | -0.810386 | 1.18E-50 | -1.69E-46 |
| MS.gene054291 | MS.gene97478 | 0.815146 | 1.07E-51 | -1.69E-46 |
| MS.gene054405 | MS.gene97478 | -0.82975 | 4.32E-55 | -1.69E-46 |
| MS.gene05510 | MS.gene97478 | -0.800686 | 1.28E-48 | -1.69E-46 |
| MS.gene055701 | MS.gene97478 | -0.823939 | 1.06E-53 | -1.69E-46 |
| MS.gene056979 | MS.gene97478 | -0.806622 | 7.52E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene97478 | -0.815817 | 7.61E-52 | -1.69E-46 |
| MS.gene060137 | MS.gene97478 | 0.832988 | 6.90E-56 | -1.69E-46 |
| MS.gene060634 | MS.gene97478 | 0.846705 | 1.84E-59 | -1.69E-46 |
| MS.gene060683 | MS.gene97478 | -0.802691 | 4.98E-49 | -1.69E-46 |
| MS.gene061040 | MS.gene97478 | 0.83119 | 1.92E-55 | -1.69E-46 |
| MS.gene061114 | MS.gene97478 | 0.845568 | 3.74E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGAGAAATTCACATTTA+AGG | 0.174751 | 7.3:-48871941 | MS.gene97478:CDS |
| TGTTATGAAGTTGATATTCT+TGG | 0.275463 | 7.3:-48872091 | MS.gene97478:CDS |
| GGACAATATGTCTTTCTTCT+TGG | 0.280895 | 7.3:-48871687 | MS.gene97478:CDS |
| AGTATTGCCCTGCTTATTGT+TGG | 0.324545 | 7.3:-48871974 | MS.gene97478:CDS |
| AGAAACATTGACAATTTAAC+AGG | 0.337581 | 7.3:-48871708 | MS.gene97478:CDS |
| GACAATATGTCTTTCTTCTT+GGG | 0.345288 | 7.3:-48871686 | MS.gene97478:intron |
| GAGCACTATACATTCTCAAC+TGG | 0.354642 | 7.3:-48870948 | MS.gene97478:CDS |
| TGAATTTCTCATTGATAAGT+AGG | 0.405613 | 7.3:+48871950 | None:intergenic |
| CAGTACCAGTTGAGGAAGTA+TGG | 0.407791 | 7.3:+48871743 | None:intergenic |
| TGGCAACAGCTTCCAAATAC+AGG | 0.408291 | 7.3:+48871763 | None:intergenic |
| GTTATGAAGTTGATATTCTT+GGG | 0.424245 | 7.3:-48872090 | MS.gene97478:CDS |
| TGTTGCCATACTTCCTCAAC+TGG | 0.450840 | 7.3:-48871748 | MS.gene97478:CDS |
| CCGAGCCCCACTTTGTCCAT+TGG | 0.453725 | 7.3:-48870909 | MS.gene97478:CDS |
| ATGCTGGCAAATGAAGCTTC+TGG | 0.490077 | 7.3:+48870584 | None:intergenic |
| AAGTAGGGCCAACAATAAGC+AGG | 0.512666 | 7.3:+48871966 | None:intergenic |
| TAGATCATATGACAAAGATC+AGG | 0.514146 | 7.3:-48872019 | MS.gene97478:CDS |
| CCAATGGACAAAGTGGGGCT+CGG | 0.529481 | 7.3:+48870909 | None:intergenic |
| TACTTATCCAATGGACAAAG+TGG | 0.529817 | 7.3:+48870902 | None:intergenic |
| TATGGAGAATTACACTATGC+TGG | 0.546192 | 7.3:+48870568 | None:intergenic |
| CTTTGTAGCAGTACCAGTTG+AGG | 0.551696 | 7.3:+48871735 | None:intergenic |
| TTTCTATTGTATGGTACATG+AGG | 0.554169 | 7.3:-48872059 | MS.gene97478:CDS |
| TCTTCTACTACTATTTCCAA+AGG | 0.567268 | 7.3:-48870708 | MS.gene97478:intron |
| TGAGAAATTCACATTTAAGG+AGG | 0.576216 | 7.3:-48871938 | MS.gene97478:intron |
| GAATTTCTCATTGATAAGTA+GGG | 0.579233 | 7.3:+48871951 | None:intergenic |
| ACTTATCCAATGGACAAAGT+GGG | 0.585109 | 7.3:+48870903 | None:intergenic |
| TACTAGAAAATAGTGACGGA+AGG | 0.599670 | 7.3:+48871993 | None:intergenic |
| ACAGAATTATACTTATCCAA+TGG | 0.602023 | 7.3:+48870893 | None:intergenic |
| GGCAACAGCTTCCAAATACA+GGG | 0.635959 | 7.3:+48871764 | None:intergenic |
| CTTATCCAATGGACAAAGTG+GGG | 0.636328 | 7.3:+48870904 | None:intergenic |
| TATACATGGAATAGGTGATG+TGG | 0.652075 | 7.3:-48871794 | MS.gene97478:intron |
| AGCACTATACATTCTCAACT+GGG | 0.652774 | 7.3:-48870947 | MS.gene97478:CDS |
| GCAATACTAGAAAATAGTGA+CGG | 0.652982 | 7.3:+48871989 | None:intergenic |
| ATGATCTACGAACAATCTTG+TGG | 0.654874 | 7.3:+48872034 | None:intergenic |
| AGTAGGGCCAACAATAAGCA+GGG | 0.670399 | 7.3:+48871967 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTATTAACATAAATAAAA+TGG | + | chr7.3:48872104-48872123 | None:intergenic | 10.0% |
| !! | ATGCAAATATATAATGTTAA+AGG | + | chr7.3:48871115-48871134 | None:intergenic | 15.0% |
| !! | CTATAATAAAAAATAAGCAA+TGG | - | chr7.3:48871682-48871701 | MS.gene97478:intron | 15.0% |
| !! | CTAGATAAATTATAGCTAAT+GGG | - | chr7.3:48871435-48871454 | MS.gene97478:intron | 20.0% |
| !! | TAAAAACATTCAAACATGTA+TGG | + | chr7.3:48870897-48870916 | None:intergenic | 20.0% |
| !! | TAGATAAATTATAGCTAATG+GGG | - | chr7.3:48871436-48871455 | MS.gene97478:intron | 20.0% |
| !!! | AGTTGAAAGTTTTTATTTGT+TGG | - | chr7.3:48871569-48871588 | MS.gene97478:intron | 20.0% |
| !!! | TCTTGTTTAATTGTTATCAT+TGG | - | chr7.3:48871895-48871914 | MS.gene97478:intron | 20.0% |
| ! | AAAGCTAAAACAAGTTCAAA+TGG | + | chr7.3:48871730-48871749 | None:intergenic | 25.0% |
| ! | AAGCTAAAACAAGTTCAAAT+GGG | + | chr7.3:48871729-48871748 | None:intergenic | 25.0% |
| ! | AATTAGCAGCTATTATATTC+TGG | - | chr7.3:48871167-48871186 | MS.gene97478:intron | 25.0% |
| ! | ACAGAATTATACTTATCCAA+TGG | + | chr7.3:48871874-48871893 | None:intergenic | 25.0% |
| ! | ACTGTAATGTTTAACAATGA+TGG | + | chr7.3:48871949-48871968 | None:intergenic | 25.0% |
| ! | AGAAACATTGACAATTTAAC+AGG | - | chr7.3:48871056-48871075 | MS.gene97478:intron | 25.0% |
| ! | ATATATTTGCATGATTTCTG+TGG | - | chr7.3:48871123-48871142 | MS.gene97478:intron | 25.0% |
| ! | ATCATTGTTAAACATTACAG+TGG | - | chr7.3:48871948-48871967 | MS.gene97478:CDS | 25.0% |
| ! | CAATGAGAAATTCACATTTA+AGG | - | chr7.3:48870823-48870842 | MS.gene97478:intron | 25.0% |
| ! | CCATTAGCTATAATTTATCT+AGG | + | chr7.3:48871437-48871456 | None:intergenic | 25.0% |
| ! | CCTAGATAAATTATAGCTAA+TGG | - | chr7.3:48871434-48871453 | MS.gene97478:intron | 25.0% |
| ! | GAATTTCTCATTGATAAGTA+GGG | + | chr7.3:48870816-48870835 | None:intergenic | 25.0% |
| ! | TACTATTTCCAAAGGTAAAT+TGG | - | chr7.3:48872064-48872083 | MS.gene97478:CDS | 25.0% |
| ! | TATAATTTATCTAGGTGAGT+TGG | + | chr7.3:48871429-48871448 | None:intergenic | 25.0% |
| ! | TGAATTTCTCATTGATAAGT+AGG | + | chr7.3:48870817-48870836 | None:intergenic | 25.0% |
| ! | TTTCATGTAACATGTTGTAT+AGG | - | chr7.3:48871387-48871406 | MS.gene97478:intron | 25.0% |
| !!! | GTTATGAAGTTGATATTCTT+GGG | - | chr7.3:48870674-48870693 | MS.gene97478:intron | 25.0% |
| !!! | TGTTATGAAGTTGATATTCT+TGG | - | chr7.3:48870673-48870692 | MS.gene97478:intron | 25.0% |
| !!! | TTGAACTTGTTTTAGCTTTT+AGG | - | chr7.3:48871731-48871750 | MS.gene97478:CDS | 25.0% |
| AAACTGCTTATACATGGAAT+AGG | - | chr7.3:48870962-48870981 | MS.gene97478:intron | 30.0% | |
| AACCTTAATAAGCAGAACTT+TGG | + | chr7.3:48871627-48871646 | None:intergenic | 30.0% | |
| ATGGTAACTTTGTATATCAG+AGG | - | chr7.3:48871701-48871720 | MS.gene97478:CDS | 30.0% | |
| CAACTCAAGATCATAATAGA+AGG | + | chr7.3:48871554-48871573 | None:intergenic | 30.0% | |
| GACAATATGTCTTTCTTCTT+GGG | - | chr7.3:48871078-48871097 | MS.gene97478:intron | 30.0% | |
| GCAATACTAGAAAATAGTGA+CGG | + | chr7.3:48870778-48870797 | None:intergenic | 30.0% | |
| TAGATCATATGACAAAGATC+AGG | - | chr7.3:48870745-48870764 | MS.gene97478:CDS | 30.0% | |
| TCTTCTACTACTATTTCCAA+AGG | - | chr7.3:48872056-48872075 | MS.gene97478:CDS | 30.0% | |
| TGAAAATATTGCCTTGTAGT+TGG | - | chr7.3:48872149-48872168 | MS.gene97478:CDS | 30.0% | |
| TGAGAAATTCACATTTAAGG+AGG | - | chr7.3:48870826-48870845 | MS.gene97478:intron | 30.0% | |
| TTTCTATTGTATGGTACATG+AGG | - | chr7.3:48870705-48870724 | MS.gene97478:intron | 30.0% | |
| ! | ATTCCATGTATAAGCAGTTT+TGG | + | chr7.3:48870962-48870981 | None:intergenic | 30.0% |
| ! | GAAGCTCATTTTCTATTGTA+TGG | - | chr7.3:48870696-48870715 | MS.gene97478:intron | 30.0% |
| ! | TTCCATGTATAAGCAGTTTT+GGG | + | chr7.3:48870961-48870980 | None:intergenic | 30.0% |
| ! | TTTATTTGTTGGAACGAAAC+TGG | - | chr7.3:48871580-48871599 | MS.gene97478:intron | 30.0% |
| ACTTATCCAATGGACAAAGT+GGG | + | chr7.3:48871864-48871883 | None:intergenic | 35.0% | |
| AGCACTATACATTCTCAACT+GGG | - | chr7.3:48871817-48871836 | MS.gene97478:intron | 35.0% | |
| AGCCAAAGTTCTGCTTATTA+AGG | - | chr7.3:48871622-48871641 | MS.gene97478:intron | 35.0% | |
| ATAAAATGGACCTGACAAGT+AGG | + | chr7.3:48872090-48872109 | None:intergenic | 35.0% | |
| ATGATCTACGAACAATCTTG+TGG | + | chr7.3:48870733-48870752 | None:intergenic | 35.0% | |
| GGACAATATGTCTTTCTTCT+TGG | - | chr7.3:48871077-48871096 | MS.gene97478:intron | 35.0% | |
| GGCTTCAAGATCATAATAGA+AGG | + | chr7.3:48871606-48871625 | None:intergenic | 35.0% | |
| TACTTATCCAATGGACAAAG+TGG | + | chr7.3:48871865-48871884 | None:intergenic | 35.0% | |
| TATACATGGAATAGGTGATG+TGG | - | chr7.3:48870970-48870989 | MS.gene97478:intron | 35.0% | |
| TTGAAACTGATCAATGAGAC+TGG | - | chr7.3:48871528-48871547 | MS.gene97478:intron | 35.0% | |
| TTGGTTTATGCTCTTTAGAC+TGG | - | chr7.3:48871914-48871933 | MS.gene97478:intron | 35.0% | |
| TTTCAATGAAGCCAACAAAC+AGG | + | chr7.3:48871514-48871533 | None:intergenic | 35.0% | |
| ! | CATTACTTCTTTTGCAGCAT+GGG | - | chr7.3:48871997-48872016 | MS.gene97478:CDS | 35.0% |
| ! | TACTAGAAAATAGTGACGGA+AGG | + | chr7.3:48870774-48870793 | None:intergenic | 35.0% |
| AATGGAAGGAAGTCAGAACA+AGG | + | chr7.3:48871981-48872000 | None:intergenic | 40.0% | |
| AGACCAACCTTCCAGTTATT+TGG | + | chr7.3:48871218-48871237 | None:intergenic | 40.0% | |
| AGTATTGCCCTGCTTATTGT+TGG | - | chr7.3:48870790-48870809 | MS.gene97478:intron | 40.0% | |
| CAAGTAGGCCAATTTACCTT+TGG | + | chr7.3:48872075-48872094 | None:intergenic | 40.0% | |
| CCATGCTGCAAAAGAAGTAA+TGG | + | chr7.3:48871999-48872018 | None:intergenic | 40.0% | |
| CTGATCAACAGCCAAATAAC+TGG | - | chr7.3:48871204-48871223 | MS.gene97478:intron | 40.0% | |
| CTTATCCAATGGACAAAGTG+GGG | + | chr7.3:48871863-48871882 | None:intergenic | 40.0% | |
| CTTTCTTCTTGGGTAAGCTA+TGG | - | chr7.3:48871088-48871107 | MS.gene97478:intron | 40.0% | |
| CTTTGTCTGTGAAATGCAGT+GGG | - | chr7.3:48871789-48871808 | MS.gene97478:intron | 40.0% | |
| GAGCACTATACATTCTCAAC+TGG | - | chr7.3:48871816-48871835 | MS.gene97478:intron | 40.0% | |
| GCTCAAAACTAAGACGAACA+AGG | + | chr7.3:48872126-48872145 | None:intergenic | 40.0% | |
| GTCCCAAAACTGCTTATACA+TGG | - | chr7.3:48870956-48870975 | MS.gene97478:CDS | 40.0% | |
| TAGCTAATGGGGACAACAAA+AGG | - | chr7.3:48871447-48871466 | MS.gene97478:intron | 40.0% | |
| TCAACAGCCAAATAACTGGA+AGG | - | chr7.3:48871208-48871227 | MS.gene97478:intron | 40.0% | |
| TGGTTGTTCTTCCAACTACA+AGG | + | chr7.3:48872163-48872182 | None:intergenic | 40.0% | |
| ! | CCATTACTTCTTTTGCAGCA+TGG | - | chr7.3:48871996-48872015 | MS.gene97478:CDS | 40.0% |
| ! | GCTGCAAAAGAAGTAATGGA+AGG | + | chr7.3:48871995-48872014 | None:intergenic | 40.0% |
| ! | GTGAGTTGGATTGACAACTT+TGG | + | chr7.3:48871415-48871434 | None:intergenic | 40.0% |
| ! | GTGGACATTTTCCCTGTATT+TGG | - | chr7.3:48870989-48871008 | MS.gene97478:intron | 40.0% |
| AAGTAGGGCCAACAATAAGC+AGG | + | chr7.3:48870801-48870820 | None:intergenic | 45.0% | |
| AGTAGGGCCAACAATAAGCA+GGG | + | chr7.3:48870800-48870819 | None:intergenic | 45.0% | |
| ATGCTGGCAAATGAAGCTTC+TGG | + | chr7.3:48872183-48872202 | None:intergenic | 45.0% | |
| CAGCCAAATAACTGGAAGGT+TGG | - | chr7.3:48871212-48871231 | MS.gene97478:intron | 45.0% | |
| CAGTACCAGTTGAGGAAGTA+TGG | + | chr7.3:48871024-48871043 | None:intergenic | 45.0% | |
| CTTTGTAGCAGTACCAGTTG+AGG | + | chr7.3:48871032-48871051 | None:intergenic | 45.0% | |
| GAAGCTTCATTTGCCAGCAT+AGG | - | chr7.3:48872183-48872202 | MS.gene97478:CDS | 45.0% | |
| GCATAGAACGAGCTTAGTAG+TGG | - | chr7.3:48871256-48871275 | MS.gene97478:intron | 45.0% | |
| GCTTTGTCTGTGAAATGCAG+TGG | - | chr7.3:48871788-48871807 | MS.gene97478:intron | 45.0% | |
| GGCAACAGCTTCCAAATACA+GGG | + | chr7.3:48871003-48871022 | None:intergenic | 45.0% | |
| TGGCAACAGCTTCCAAATAC+AGG | + | chr7.3:48871004-48871023 | None:intergenic | 45.0% | |
| TGTTGCCATACTTCCTCAAC+TGG | - | chr7.3:48871016-48871035 | MS.gene97478:intron | 45.0% | |
| ! | CTTTTGCAGCATGGGTTTCT+GGG | - | chr7.3:48872005-48872024 | MS.gene97478:CDS | 45.0% |
| ! | GGTAAATTGGCCTACTTGTC+AGG | - | chr7.3:48872077-48872096 | MS.gene97478:CDS | 45.0% |
| ! | TCTTTTGCAGCATGGGTTTC+TGG | - | chr7.3:48872004-48872023 | MS.gene97478:CDS | 45.0% |
| !! | TTAGTTTGCAGTAGTGCTCG+TGG | - | chr7.3:48871483-48871502 | MS.gene97478:intron | 45.0% |
| TCGTGGACAAGCCTGTTTGT+TGG | - | chr7.3:48871500-48871519 | MS.gene97478:intron | 50.0% | |
| CCAATGGACAAAGTGGGGCT+CGG | + | chr7.3:48871858-48871877 | None:intergenic | 55.0% | |
| CCGAGCCCCACTTTGTCCAT+TGG | - | chr7.3:48871855-48871874 | MS.gene97478:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 48870582 | 48872204 | 48870582 | ID=MS.gene97478 |
| chr7.3 | mRNA | 48870582 | 48872204 | 48870582 | ID=MS.gene97478.t1;Parent=MS.gene97478 |
| chr7.3 | exon | 48871939 | 48872204 | 48871939 | ID=MS.gene97478.t1.exon1;Parent=MS.gene97478.t1 |
| chr7.3 | CDS | 48871939 | 48872204 | 48871939 | ID=cds.MS.gene97478.t1;Parent=MS.gene97478.t1 |
| chr7.3 | exon | 48871687 | 48871802 | 48871687 | ID=MS.gene97478.t1.exon2;Parent=MS.gene97478.t1 |
| chr7.3 | CDS | 48871687 | 48871802 | 48871687 | ID=cds.MS.gene97478.t1;Parent=MS.gene97478.t1 |
| chr7.3 | exon | 48870905 | 48870978 | 48870905 | ID=MS.gene97478.t1.exon3;Parent=MS.gene97478.t1 |
| chr7.3 | CDS | 48870905 | 48870978 | 48870905 | ID=cds.MS.gene97478.t1;Parent=MS.gene97478.t1 |
| chr7.3 | exon | 48870709 | 48870772 | 48870709 | ID=MS.gene97478.t1.exon4;Parent=MS.gene97478.t1 |
| chr7.3 | CDS | 48870709 | 48870772 | 48870709 | ID=cds.MS.gene97478.t1;Parent=MS.gene97478.t1 |
| chr7.3 | exon | 48870582 | 48870618 | 48870582 | ID=MS.gene97478.t1.exon5;Parent=MS.gene97478.t1 |
| chr7.3 | CDS | 48870582 | 48870618 | 48870582 | ID=cds.MS.gene97478.t1;Parent=MS.gene97478.t1 |
| Gene Sequence |
| Protein sequence |
