Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98765.t1 | KEH25024.1 | 79.1 | 177 | 23 | 1 | 72 | 248 | 50 | 212 | 1.30E-74 | 289.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98765.t1 | A0A072U5J3 | 79.1 | 177 | 23 | 1 | 72 | 248 | 50 | 212 | 9.6e-75 | 289.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene98765 | 0.809632 | 1.72E-50 | -1.69E-46 |
| MS.gene050237 | MS.gene98765 | 0.808474 | 3.04E-50 | -1.69E-46 |
| MS.gene050750 | MS.gene98765 | 0.805141 | 1.54E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene98765 | 0.80599 | 1.02E-49 | -1.69E-46 |
| MS.gene052584 | MS.gene98765 | 0.801255 | 9.81E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene98765 | 0.812696 | 3.72E-51 | -1.69E-46 |
| MS.gene05415 | MS.gene98765 | 0.8106 | 1.06E-50 | -1.69E-46 |
| MS.gene054657 | MS.gene98765 | 0.808432 | 3.11E-50 | -1.69E-46 |
| MS.gene056498 | MS.gene98765 | 0.804561 | 2.04E-49 | -1.69E-46 |
| MS.gene056776 | MS.gene98765 | 0.802018 | 6.84E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene98765 | 0.806543 | 7.82E-50 | -1.69E-46 |
| MS.gene057444 | MS.gene98765 | 0.800036 | 1.74E-48 | -1.69E-46 |
| MS.gene058548 | MS.gene98765 | 0.812525 | 4.06E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene98765 | 0.80054 | 1.37E-48 | -1.69E-46 |
| MS.gene060310 | MS.gene98765 | 0.807974 | 3.89E-50 | -1.69E-46 |
| MS.gene060634 | MS.gene98765 | -0.819874 | 9.23E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98765.t1 | MTR_6g013220 | 79.070 | 172 | 22 | 1 | 77 | 248 | 55 | 212 | 2.15e-96 | 281 |
| MS.gene98765.t1 | MTR_6g048070 | 45.545 | 101 | 37 | 2 | 1 | 85 | 1 | 99 | 1.29e-19 | 82.0 |
| MS.gene98765.t1 | MTR_6g016175 | 26.295 | 251 | 158 | 8 | 1 | 243 | 1 | 232 | 1.24e-15 | 76.3 |
| MS.gene98765.t1 | MTR_3g448380 | 30.625 | 160 | 107 | 3 | 59 | 214 | 117 | 276 | 2.84e-14 | 71.2 |
| MS.gene98765.t1 | MTR_1g040450 | 30.409 | 171 | 113 | 4 | 76 | 243 | 111 | 278 | 7.38e-14 | 70.5 |
| MS.gene98765.t1 | MTR_3g022950 | 26.289 | 194 | 133 | 4 | 3 | 193 | 636 | 822 | 1.35e-13 | 70.5 |
| MS.gene98765.t1 | MTR_6g048840 | 28.000 | 175 | 112 | 5 | 76 | 243 | 225 | 392 | 2.08e-13 | 69.3 |
| MS.gene98765.t1 | MTR_8g058470 | 25.926 | 189 | 133 | 4 | 59 | 243 | 355 | 540 | 2.25e-13 | 69.7 |
| MS.gene98765.t1 | MTR_7g108760 | 25.926 | 189 | 133 | 4 | 59 | 243 | 357 | 542 | 2.25e-13 | 69.7 |
| MS.gene98765.t1 | MTR_2g105880 | 23.950 | 238 | 166 | 5 | 10 | 243 | 67 | 293 | 7.58e-13 | 67.4 |
| MS.gene98765.t1 | MTR_1g054775 | 29.885 | 174 | 104 | 5 | 76 | 238 | 618 | 784 | 7.56e-12 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCAAGAGAGATTTGATTTA+TGG | 0.116291 | 6.2:+22692368 | MS.gene98765:CDS |
| TCGTCATTGGAACATGTTTC+TGG | 0.158342 | 6.2:-22692190 | None:intergenic |
| AAATCGCCAAACATCATTAA+AGG | 0.271490 | 6.2:-22692427 | None:intergenic |
| TCTTGACGTGTCTTTCCTAT+TGG | 0.326012 | 6.2:+22692327 | MS.gene98765:CDS |
| TCAACACCTTTAATGATGTT+TGG | 0.344659 | 6.2:+22692421 | MS.gene98765:CDS |
| TACACCTATGAGCAACCTTA+TGG | 0.346601 | 6.2:-22692063 | None:intergenic |
| TCATTACCCTTTAGTTCTTC+CGG | 0.360197 | 6.2:-22692466 | None:intergenic |
| AACAAATAAGAGATACATCT+TGG | 0.380955 | 6.2:+22692279 | MS.gene98765:CDS |
| TTGACCAAGCCCAACACAAT+TGG | 0.391633 | 6.2:-22692088 | None:intergenic |
| CGCCCTCTATTAGTTCAATC+TGG | 0.398255 | 6.2:+22692697 | MS.gene98765:CDS |
| CCTTGTGGAGCTTCCGTTGT+TGG | 0.424252 | 6.2:+22692540 | MS.gene98765:CDS |
| GACAGGGATTGGTATTCTCT+TGG | 0.442717 | 6.2:+22692224 | MS.gene98765:CDS |
| GAACCAGATTGAACTAATAG+AGG | 0.449982 | 6.2:-22692700 | None:intergenic |
| GTAGGAGGGATTACTGAGTT+AGG | 0.459744 | 6.2:+22692025 | MS.gene98765:CDS |
| TAATCCAATTGTGTTGGGCT+TGG | 0.460396 | 6.2:+22692084 | MS.gene98765:CDS |
| TTTGGAACCGGAAGAACTAA+AGG | 0.463362 | 6.2:+22692459 | MS.gene98765:CDS |
| AGGTGTAATCCAATTGTGTT+GGG | 0.478595 | 6.2:+22692079 | MS.gene98765:CDS |
| AACTCGGTGCATGAACCAAT+AGG | 0.486014 | 6.2:-22692342 | None:intergenic |
| AGGAGGAGAACAATGTAAGC+TGG | 0.493023 | 6.2:+22692158 | MS.gene98765:CDS |
| TTGGAACCGGAAGAACTAAA+GGG | 0.493607 | 6.2:+22692460 | MS.gene98765:CDS |
| AAGAGATACATCTTGGTTGA+AGG | 0.497593 | 6.2:+22692286 | MS.gene98765:CDS |
| GAAGGAAAATTGATTCAAGA+AGG | 0.514667 | 6.2:+22692304 | MS.gene98765:CDS |
| GGAATGGCTAGAGCATTTCA+AGG | 0.514887 | 6.2:+22692633 | MS.gene98765:CDS |
| CTCTATCTATTCTGCCTGCA+CGG | 0.531142 | 6.2:-22692597 | None:intergenic |
| TGATCCATAAGGTTGCTCAT+AGG | 0.532353 | 6.2:+22692059 | MS.gene98765:CDS |
| TAGGTGTAATCCAATTGTGT+TGG | 0.533505 | 6.2:+22692078 | MS.gene98765:CDS |
| GACTATGACAATAACCGTGC+AGG | 0.543680 | 6.2:+22692583 | MS.gene98765:CDS |
| TGTTCCAATGACGAGAAAAG+TGG | 0.545436 | 6.2:+22692199 | MS.gene98765:CDS |
| GTTCCAATGACGAGAAAAGT+GGG | 0.548017 | 6.2:+22692200 | MS.gene98765:CDS |
| AGTTAAAGGATGGTGGAAGG+AGG | 0.557788 | 6.2:+22692141 | MS.gene98765:CDS |
| AAAATCTGCGATGATCCATA+AGG | 0.558872 | 6.2:+22692048 | MS.gene98765:CDS |
| GATGACATTACAAGCGTATG+TGG | 0.559882 | 6.2:+22692727 | MS.gene98765:CDS |
| GGTAATGAGGTGACTTCACA+AGG | 0.574743 | 6.2:+22692481 | MS.gene98765:CDS |
| GGAGGAGAACAATGTAAGCT+GGG | 0.576028 | 6.2:+22692159 | MS.gene98765:CDS |
| TCATTTCAGCTATACAATGT+CGG | 0.588108 | 6.2:-22692516 | None:intergenic |
| AATCTCTCTTGACTGCAACT+CGG | 0.607370 | 6.2:-22692358 | None:intergenic |
| GGAAGAACTAAAGGGTAATG+AGG | 0.610801 | 6.2:+22692468 | MS.gene98765:CDS |
| AAAAGTTAAAGGATGGTGGA+AGG | 0.637401 | 6.2:+22692138 | MS.gene98765:CDS |
| AACCAGATTGAACTAATAGA+GGG | 0.638427 | 6.2:-22692699 | None:intergenic |
| GTGAACTTTCTGTCCAACAA+CGG | 0.668255 | 6.2:-22692553 | None:intergenic |
| TCCAATGACGAGAAAAGTGG+GGG | 0.678217 | 6.2:+22692202 | MS.gene98765:CDS |
| TTCCAATGACGAGAAAAGTG+GGG | 0.679735 | 6.2:+22692201 | MS.gene98765:CDS |
| TGTTGGACAGAAAGTTCACA+TGG | 0.688863 | 6.2:+22692557 | MS.gene98765:CDS |
| CCAACAACGGAAGCTCCACA+AGG | 0.688911 | 6.2:-22692540 | None:intergenic |
| TATAGCTGAAATGAACCTTG+TGG | 0.699115 | 6.2:+22692525 | MS.gene98765:CDS |
| CAGATTGAACTAATAGAGGG+CGG | 0.706620 | 6.2:-22692696 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAAATAAGAGATACATCT+TGG | + | chr6.2:22692279-22692298 | MS.gene98765:CDS | 25.0% |
| AAATCGCCAAACATCATTAA+AGG | - | chr6.2:22692430-22692449 | None:intergenic | 30.0% | |
| AACCAGATTGAACTAATAGA+GGG | - | chr6.2:22692702-22692721 | None:intergenic | 30.0% | |
| ATGAAAAAGTTAAAGGATGG+TGG | + | chr6.2:22692134-22692153 | MS.gene98765:CDS | 30.0% | |
| TCAACACCTTTAATGATGTT+TGG | + | chr6.2:22692421-22692440 | MS.gene98765:CDS | 30.0% | |
| TCATTTCAGCTATACAATGT+CGG | - | chr6.2:22692519-22692538 | None:intergenic | 30.0% | |
| ! | AGATAGAGTTTTTGTAGATC+AGG | + | chr6.2:22692612-22692631 | MS.gene98765:CDS | 30.0% |
| ! | GAAGGAAAATTGATTCAAGA+AGG | + | chr6.2:22692304-22692323 | MS.gene98765:CDS | 30.0% |
| ! | GTCAAGAGAGATTTGATTTA+TGG | + | chr6.2:22692368-22692387 | MS.gene98765:CDS | 30.0% |
| !!! | CCATCCTTTAACTTTTTCAT+CGG | - | chr6.2:22692134-22692153 | None:intergenic | 30.0% |
| !!! | TTTTCAAGAAGTTTTGGAAC+CGG | + | chr6.2:22692447-22692466 | MS.gene98765:CDS | 30.0% |
| AAAATCTGCGATGATCCATA+AGG | + | chr6.2:22692048-22692067 | MS.gene98765:CDS | 35.0% | |
| AAGAGATACATCTTGGTTGA+AGG | + | chr6.2:22692286-22692305 | MS.gene98765:CDS | 35.0% | |
| AGGTGTAATCCAATTGTGTT+GGG | + | chr6.2:22692079-22692098 | MS.gene98765:CDS | 35.0% | |
| CCGATGAAAAAGTTAAAGGA+TGG | + | chr6.2:22692131-22692150 | MS.gene98765:CDS | 35.0% | |
| CTCACCGATGAAAAAGTTAA+AGG | + | chr6.2:22692127-22692146 | MS.gene98765:CDS | 35.0% | |
| GAACCAGATTGAACTAATAG+AGG | - | chr6.2:22692703-22692722 | None:intergenic | 35.0% | |
| TAGGTGTAATCCAATTGTGT+TGG | + | chr6.2:22692078-22692097 | MS.gene98765:CDS | 35.0% | |
| TATAGCTGAAATGAACCTTG+TGG | + | chr6.2:22692525-22692544 | MS.gene98765:CDS | 35.0% | |
| ! | GAGTTTTTGTAGATCAGGAA+TGG | + | chr6.2:22692617-22692636 | MS.gene98765:CDS | 35.0% |
| !! | AAAAGTTAAAGGATGGTGGA+AGG | + | chr6.2:22692138-22692157 | MS.gene98765:CDS | 35.0% |
| !! | TCATTACCCTTTAGTTCTTC+CGG | - | chr6.2:22692469-22692488 | None:intergenic | 35.0% |
| !! | TGGCGATTTTCAAGAAGTTT+TGG | + | chr6.2:22692441-22692460 | MS.gene98765:CDS | 35.0% |
| !!! | AGGATTTTAGGTTGGAATGT+AGG | + | chr6.2:22692007-22692026 | MS.gene98765:CDS | 35.0% |
| !!! | ATTTCAAGGTTTTGAAGCTG+CGG | + | chr6.2:22692647-22692666 | MS.gene98765:CDS | 35.0% |
| !!! | ATTTTAGGTTGGAATGTAGG+AGG | + | chr6.2:22692010-22692029 | MS.gene98765:CDS | 35.0% |
| !!! | TTTTAGGTTGGAATGTAGGA+GGG | + | chr6.2:22692011-22692030 | MS.gene98765:CDS | 35.0% |
| AATCTCTCTTGACTGCAACT+CGG | - | chr6.2:22692361-22692380 | None:intergenic | 40.0% | |
| CAGATTGAACTAATAGAGGG+CGG | - | chr6.2:22692699-22692718 | None:intergenic | 40.0% | |
| GATGACATTACAAGCGTATG+TGG | + | chr6.2:22692727-22692746 | MS.gene98765:CDS | 40.0% | |
| GGAAGAACTAAAGGGTAATG+AGG | + | chr6.2:22692468-22692487 | MS.gene98765:CDS | 40.0% | |
| GTGAACTTTCTGTCCAACAA+CGG | - | chr6.2:22692556-22692575 | None:intergenic | 40.0% | |
| GTTCCAATGACGAGAAAAGT+GGG | + | chr6.2:22692200-22692219 | MS.gene98765:CDS | 40.0% | |
| TAATCCAATTGTGTTGGGCT+TGG | + | chr6.2:22692084-22692103 | MS.gene98765:CDS | 40.0% | |
| TACACCTATGAGCAACCTTA+TGG | - | chr6.2:22692066-22692085 | None:intergenic | 40.0% | |
| TCGTCATTGGAACATGTTTC+TGG | - | chr6.2:22692193-22692212 | None:intergenic | 40.0% | |
| TCTTGACGTGTCTTTCCTAT+TGG | + | chr6.2:22692327-22692346 | MS.gene98765:CDS | 40.0% | |
| TGATCCATAAGGTTGCTCAT+AGG | + | chr6.2:22692059-22692078 | MS.gene98765:CDS | 40.0% | |
| TGTTCCAATGACGAGAAAAG+TGG | + | chr6.2:22692199-22692218 | MS.gene98765:CDS | 40.0% | |
| TGTTGGACAGAAAGTTCACA+TGG | + | chr6.2:22692557-22692576 | MS.gene98765:CDS | 40.0% | |
| TTCCAATGACGAGAAAAGTG+GGG | + | chr6.2:22692201-22692220 | MS.gene98765:CDS | 40.0% | |
| TTGGAACCGGAAGAACTAAA+GGG | + | chr6.2:22692460-22692479 | MS.gene98765:CDS | 40.0% | |
| ! | TTTGGAACCGGAAGAACTAA+AGG | + | chr6.2:22692459-22692478 | MS.gene98765:CDS | 40.0% |
| !!! | TTTCAAGGTTTTGAAGCTGC+GGG | + | chr6.2:22692648-22692667 | MS.gene98765:CDS | 40.0% |
| AGGAGGAGAACAATGTAAGC+TGG | + | chr6.2:22692158-22692177 | MS.gene98765:CDS | 45.0% | |
| CGCCCTCTATTAGTTCAATC+TGG | + | chr6.2:22692697-22692716 | MS.gene98765:CDS | 45.0% | |
| CTCTATCTATTCTGCCTGCA+CGG | - | chr6.2:22692600-22692619 | None:intergenic | 45.0% | |
| GACTATGACAATAACCGTGC+AGG | + | chr6.2:22692583-22692602 | MS.gene98765:CDS | 45.0% | |
| GGAGGAGAACAATGTAAGCT+GGG | + | chr6.2:22692159-22692178 | MS.gene98765:CDS | 45.0% | |
| GGTAATGAGGTGACTTCACA+AGG | + | chr6.2:22692481-22692500 | MS.gene98765:CDS | 45.0% | |
| GTAGGAGGGATTACTGAGTT+AGG | + | chr6.2:22692025-22692044 | MS.gene98765:CDS | 45.0% | |
| TCCAATGACGAGAAAAGTGG+GGG | + | chr6.2:22692202-22692221 | MS.gene98765:CDS | 45.0% | |
| TTGACCAAGCCCAACACAAT+TGG | - | chr6.2:22692091-22692110 | None:intergenic | 45.0% | |
| ! | GACAGGGATTGGTATTCTCT+TGG | + | chr6.2:22692224-22692243 | MS.gene98765:CDS | 45.0% |
| ! | GGAATGGCTAGAGCATTTCA+AGG | + | chr6.2:22692633-22692652 | MS.gene98765:CDS | 45.0% |
| !! | AACTCGGTGCATGAACCAAT+AGG | - | chr6.2:22692345-22692364 | None:intergenic | 45.0% |
| !! | AGTTAAAGGATGGTGGAAGG+AGG | + | chr6.2:22692141-22692160 | MS.gene98765:CDS | 45.0% |
| !!! | TTCAAGGTTTTGAAGCTGCG+GGG | + | chr6.2:22692649-22692668 | MS.gene98765:CDS | 45.0% |
| !! | TCCCCCACTTTTCTCGTCAT+TGG | - | chr6.2:22692206-22692225 | None:intergenic | 50.0% |
| CCAACAACGGAAGCTCCACA+AGG | - | chr6.2:22692543-22692562 | None:intergenic | 55.0% | |
| CCTTGTGGAGCTTCCGTTGT+TGG | + | chr6.2:22692540-22692559 | MS.gene98765:CDS | 55.0% | |
| GAAAAGTGGGGGACAGGGAT+TGG | + | chr6.2:22692213-22692232 | MS.gene98765:CDS | 55.0% | |
| GACGAGAAAAGTGGGGGACA+GGG | + | chr6.2:22692208-22692227 | MS.gene98765:CDS | 55.0% | |
| TGACGAGAAAAGTGGGGGAC+AGG | + | chr6.2:22692207-22692226 | MS.gene98765:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 22692004 | 22692756 | 22692004 | ID=MS.gene98765 |
| chr6.2 | mRNA | 22692004 | 22692756 | 22692004 | ID=MS.gene98765.t1;Parent=MS.gene98765 |
| chr6.2 | exon | 22692004 | 22692756 | 22692004 | ID=MS.gene98765.t1.exon1;Parent=MS.gene98765.t1 |
| chr6.2 | CDS | 22692004 | 22692756 | 22692004 | ID=cds.MS.gene98765.t1;Parent=MS.gene98765.t1 |
| Gene Sequence |
| Protein sequence |
