Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99607.t1 | GAU38796.1 | 85.1 | 134 | 9 | 2 | 1 | 123 | 1 | 134 | 6.00E-52 | 213.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99607.t1 | A0A2Z6N4K6 | 85.1 | 134 | 9 | 2 | 1 | 123 | 1 | 134 | 4.3e-52 | 213.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050076 | MS.gene99607 | 0.808329 | 3.27E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene99607 | 0.813184 | 2.91E-51 | -1.69E-46 |
| MS.gene050767 | MS.gene99607 | 0.831744 | 1.40E-55 | -1.69E-46 |
| MS.gene050770 | MS.gene99607 | 0.835855 | 1.32E-56 | -1.69E-46 |
| MS.gene05101 | MS.gene99607 | 0.821096 | 4.84E-53 | -1.69E-46 |
| MS.gene052290 | MS.gene99607 | 0.807014 | 6.22E-50 | -1.69E-46 |
| MS.gene053322 | MS.gene99607 | 0.807222 | 5.62E-50 | -1.69E-46 |
| MS.gene054376 | MS.gene99607 | 0.831072 | 2.05E-55 | -1.69E-46 |
| MS.gene055254 | MS.gene99607 | 0.808344 | 3.24E-50 | -1.69E-46 |
| MS.gene055277 | MS.gene99607 | 0.810509 | 1.11E-50 | -1.69E-46 |
| MS.gene05592 | MS.gene99607 | 0.800291 | 1.54E-48 | -1.69E-46 |
| MS.gene059326 | MS.gene99607 | 0.827317 | 1.67E-54 | -1.69E-46 |
| MS.gene059894 | MS.gene99607 | 0.802349 | 5.85E-49 | -1.69E-46 |
| MS.gene059960 | MS.gene99607 | 0.806033 | 1.00E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99607.t1 | MTR_7g062470 | 90.076 | 131 | 5 | 1 | 1 | 123 | 1 | 131 | 6.56e-68 | 201 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99607.t1 | AT5G02160 | 83.696 | 92 | 15 | 0 | 32 | 123 | 37 | 128 | 3.55e-47 | 148 |
Find 28 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCTTGTTGCTGGTGCTAT+TGG | 0.295597 | 7.1:+39129863 | MS.gene99607:CDS |
| GGCACTATTTATCTTGTTGC+TGG | 0.299445 | 7.1:+39129854 | MS.gene99607:CDS |
| GTGATGGAGCAGGGTTTGTT+AGG | 0.314720 | 7.1:+39131221 | MS.gene99607:CDS |
| AAATGAGAGTTTAGTGTTGT+TGG | 0.340680 | 7.1:-39129750 | None:intergenic |
| AACCCTGCTCCATCACAATC+TGG | 0.357172 | 7.1:-39131214 | None:intergenic |
| GCTATTGGAATTGCTCTTGT+TGG | 0.368974 | 7.1:+39129878 | MS.gene99607:CDS |
| AACAACACTAAACTCTCATT+TGG | 0.372290 | 7.1:+39129752 | MS.gene99607:CDS |
| TTGTGCTCGTTGTGATGGTT+TGG | 0.405035 | 7.1:+39131300 | MS.gene99607:CDS |
| AGCATTTCTATTTGTCAACT+TGG | 0.409660 | 7.1:-39131333 | None:intergenic |
| ATTGGGAATGATTGTGTTCA+AGG | 0.420585 | 7.1:-39129717 | None:intergenic |
| TCAGTTGTTGTTTCAGCAGT+TGG | 0.426749 | 7.1:+39129815 | MS.gene99607:CDS |
| TGTGCTCGTTGTGATGGTTT+GGG | 0.434742 | 7.1:+39131301 | MS.gene99607:CDS |
| TCTTTACGTGAGAAGATGAT+AGG | 0.453999 | 7.1:-39129911 | None:intergenic |
| CAGGGTTTGTTAGGAAAGGT+GGG | 0.459410 | 7.1:+39131230 | MS.gene99607:CDS |
| GCAGGGTTTGTTAGGAAAGG+TGG | 0.460825 | 7.1:+39131229 | MS.gene99607:CDS |
| GAGAGCAAATGCAGCAAGAA+AGG | 0.478276 | 7.1:+39131261 | MS.gene99607:CDS |
| GTTGTTGGAAATTGTAACAT+TGG | 0.483393 | 7.1:-39129735 | None:intergenic |
| TGCCCAGATTGTGATGGAGC+AGG | 0.494129 | 7.1:+39131211 | MS.gene99607:CDS |
| AACACTAAACTCTCATTTGG+AGG | 0.496202 | 7.1:+39129755 | MS.gene99607:CDS |
| TTGTTGGAAATTGTAACATT+GGG | 0.522805 | 7.1:-39129734 | None:intergenic |
| ACCCTGCTCCATCACAATCT+GGG | 0.529792 | 7.1:-39131213 | None:intergenic |
| ATTGTTTGTGCTCGTTGTGA+TGG | 0.530028 | 7.1:+39131295 | MS.gene99607:CDS |
| GGAGCAGGGTTTGTTAGGAA+AGG | 0.545979 | 7.1:+39131226 | MS.gene99607:CDS |
| AATGATTGTGTTCAAGGTCA+TGG | 0.560862 | 7.1:-39129711 | None:intergenic |
| GCCCAGATTGTGATGGAGCA+GGG | 0.567834 | 7.1:+39131212 | MS.gene99607:CDS |
| GTTGGAGATGTATCTTCTGA+TGG | 0.593310 | 7.1:+39129833 | MS.gene99607:CDS |
| ATCAGATGCCCAGATTGTGA+TGG | 0.661235 | 7.1:+39131205 | MS.gene99607:intron |
| AGGGTTTGTTAGGAAAGGTG+GGG | 0.670666 | 7.1:+39131231 | MS.gene99607:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTATAAATATTTATATT+AGG | - | chr7.1:39130574-39130593 | None:intergenic | 0.0% |
| !! | AAAAAAATAAAATCTGATTT+CGG | + | chr7.1:39130394-39130413 | MS.gene99607:intron | 10.0% |
| !! | AAAAATAGGTTTATAATTAT+TGG | - | chr7.1:39131001-39131020 | None:intergenic | 10.0% |
| !! | AATAATAATCTCAATAATAG+TGG | + | chr7.1:39130599-39130618 | MS.gene99607:intron | 15.0% |
| !!! | TAATTATAAACCTATTTTTG+TGG | + | chr7.1:39131002-39131021 | MS.gene99607:intron | 15.0% |
| !! | AATCTTAAAACCACAAAAAT+AGG | - | chr7.1:39131015-39131034 | None:intergenic | 20.0% |
| !!! | AAATTTTGTATAGGCATTTT+TGG | + | chr7.1:39130125-39130144 | MS.gene99607:intron | 20.0% |
| !!! | GAATTGTTTTTTTTTTTTCG+TGG | - | chr7.1:39130537-39130556 | None:intergenic | 20.0% |
| !!! | TTCTAACAGAAATTTTGTAT+AGG | + | chr7.1:39130116-39130135 | MS.gene99607:intron | 20.0% |
| !!! | TTTTTTCATTGATTCTTGTT+TGG | + | chr7.1:39131104-39131123 | MS.gene99607:intron | 20.0% |
| ! | ACATCATTAACAAACAACAA+AGG | - | chr7.1:39131168-39131187 | None:intergenic | 25.0% |
| ! | GGTAAAACTATACTTATCTA+TGG | - | chr7.1:39130328-39130347 | None:intergenic | 25.0% |
| ! | TGCACTTGAAATAAAAATCT+AGG | - | chr7.1:39131080-39131099 | None:intergenic | 25.0% |
| ! | TTGTTGGAAATTGTAACATT+GGG | - | chr7.1:39129737-39129756 | None:intergenic | 25.0% |
| !! | ATTCTGAGAAAATTAGAGAT+AGG | - | chr7.1:39130212-39130231 | None:intergenic | 25.0% |
| !!! | ACCTAGTTTTTTAATCTACT+TGG | + | chr7.1:39131043-39131062 | MS.gene99607:intron | 25.0% |
| !!! | CATCGTTATTTTCATTTTCA+TGG | + | chr7.1:39129948-39129967 | MS.gene99607:intron | 25.0% |
| !!! | CCTAGTTTTTTAATCTACTT+GGG | + | chr7.1:39131044-39131063 | MS.gene99607:intron | 25.0% |
| !!! | TAAGTATAGTTTTACCTTGA+CGG | + | chr7.1:39130332-39130351 | MS.gene99607:intron | 25.0% |
| AAATGAGAGTTTAGTGTTGT+TGG | - | chr7.1:39129753-39129772 | None:intergenic | 30.0% | |
| AACAACACTAAACTCTCATT+TGG | + | chr7.1:39129752-39129771 | MS.gene99607:CDS | 30.0% | |
| ACAATGATCAGAGTGATAAT+TGG | + | chr7.1:39130689-39130708 | MS.gene99607:intron | 30.0% | |
| CCCAAGTAGATTAAAAAACT+AGG | - | chr7.1:39131047-39131066 | None:intergenic | 30.0% | |
| GACAATGCATAATATATGCA+AGG | + | chr7.1:39130489-39130508 | MS.gene99607:intron | 30.0% | |
| GTTGTTGGAAATTGTAACAT+TGG | - | chr7.1:39129738-39129757 | None:intergenic | 30.0% | |
| TCAATAAAAAACATCCGTCA+AGG | - | chr7.1:39130349-39130368 | None:intergenic | 30.0% | |
| TGAACAAATTACACTTGCAA+TGG | + | chr7.1:39130058-39130077 | MS.gene99607:intron | 30.0% | |
| TGATAATTGGCAATGATGAT+TGG | + | chr7.1:39130702-39130721 | MS.gene99607:intron | 30.0% | |
| TTAATTGAACTTTGCTCAGT+TGG | + | chr7.1:39130462-39130481 | MS.gene99607:intron | 30.0% | |
| !! | TCATTGATTCTTGTTTGGAA+TGG | + | chr7.1:39131109-39131128 | MS.gene99607:intron | 30.0% |
| !!! | GTGGTCATAGTAGTTTTAAA+TGG | + | chr7.1:39130794-39130813 | MS.gene99607:intron | 30.0% |
| AACACTAAACTCTCATTTGG+AGG | + | chr7.1:39129755-39129774 | MS.gene99607:CDS | 35.0% | |
| AATGATTGTGTTCAAGGTCA+TGG | - | chr7.1:39129714-39129733 | None:intergenic | 35.0% | |
| ATTGGGAATGATTGTGTTCA+AGG | - | chr7.1:39129720-39129739 | None:intergenic | 35.0% | |
| TAATTGGCAATGATGATTGG+CGG | + | chr7.1:39130705-39130724 | MS.gene99607:intron | 35.0% | |
| TCTTTACGTGAGAAGATGAT+AGG | - | chr7.1:39129914-39129933 | None:intergenic | 35.0% | |
| TGCATAATATATGCAAGGTC+CGG | + | chr7.1:39130494-39130513 | MS.gene99607:intron | 35.0% | |
| TGGTTGAAACTAGTTGTTGT+TGG | + | chr7.1:39130619-39130638 | MS.gene99607:intron | 35.0% | |
| ! | TTGAAACTAGTTGTTGTTGG+AGG | + | chr7.1:39130622-39130641 | MS.gene99607:intron | 35.0% |
| !! | GTTTTAAATGGTGATCACAG+TGG | + | chr7.1:39130806-39130825 | MS.gene99607:intron | 35.0% |
| AACGTTTCATGTTACCACCA+AGG | - | chr7.1:39130969-39130988 | None:intergenic | 40.0% | |
| ACGATCAGAATGATGATTGG+TGG | + | chr7.1:39130741-39130760 | MS.gene99607:intron | 40.0% | |
| AGTGGTGATCAGAGTATATG+TGG | + | chr7.1:39130824-39130843 | MS.gene99607:intron | 40.0% | |
| ATCTACGAAAGTTGGAGTGA+TGG | + | chr7.1:39130880-39130899 | MS.gene99607:intron | 40.0% | |
| ATTGTTTGTGCTCGTTGTGA+TGG | + | chr7.1:39131295-39131314 | MS.gene99607:CDS | 40.0% | |
| CATAATATATGCAAGGTCCG+GGG | + | chr7.1:39130496-39130515 | MS.gene99607:intron | 40.0% | |
| GCAACGATCAGAATGATGAT+TGG | + | chr7.1:39130738-39130757 | MS.gene99607:intron | 40.0% | |
| GCATAATATATGCAAGGTCC+GGG | + | chr7.1:39130495-39130514 | MS.gene99607:intron | 40.0% | |
| GCTATTGGAATTGCTCTTGT+TGG | + | chr7.1:39129878-39129897 | MS.gene99607:CDS | 40.0% | |
| GGCACTATTTATCTTGTTGC+TGG | + | chr7.1:39129854-39129873 | MS.gene99607:CDS | 40.0% | |
| GTTGGAGATGTATCTTCTGA+TGG | + | chr7.1:39129833-39129852 | MS.gene99607:CDS | 40.0% | |
| TAGAGGTGATCTACGAAAGT+TGG | + | chr7.1:39130872-39130891 | MS.gene99607:intron | 40.0% | |
| TTGAACTTTGCTCAGTTGGT+AGG | + | chr7.1:39130466-39130485 | MS.gene99607:intron | 40.0% | |
| ! | AAACTAGTTGTTGTTGGAGG+AGG | + | chr7.1:39130625-39130644 | MS.gene99607:intron | 40.0% |
| ! | AGAATGATGATTGGTGGACA+AGG | + | chr7.1:39130747-39130766 | MS.gene99607:intron | 40.0% |
| ! | GAATGATGATTGGTGGACAA+GGG | + | chr7.1:39130748-39130767 | MS.gene99607:intron | 40.0% |
| ! | TGAACTTTGCTCAGTTGGTA+GGG | + | chr7.1:39130467-39130486 | MS.gene99607:intron | 40.0% |
| !! | GAGTATATGTGGTGTCATAG+TGG | + | chr7.1:39130835-39130854 | MS.gene99607:intron | 40.0% |
| !! | TATATGTGGTGTCATAGTGG+TGG | + | chr7.1:39130838-39130857 | MS.gene99607:intron | 40.0% |
| !! | TATCTTGTTGCTGGTGCTAT+TGG | + | chr7.1:39129863-39129882 | MS.gene99607:CDS | 40.0% |
| !! | TCAGTTGTTGTTTCAGCAGT+TGG | + | chr7.1:39129815-39129834 | MS.gene99607:CDS | 40.0% |
| !!! | TTTTTTTTTCGTGGTAGCCG+AGG | - | chr7.1:39130528-39130547 | None:intergenic | 40.0% |
| AGTTGGAGTGATGGTTGACT+TGG | + | chr7.1:39130889-39130908 | MS.gene99607:intron | 45.0% | |
| ATCAGATGCCCAGATTGTGA+TGG | + | chr7.1:39131205-39131224 | MS.gene99607:intron | 45.0% | |
| GAGAGCAAATGCAGCAAGAA+AGG | + | chr7.1:39131261-39131280 | MS.gene99607:CDS | 45.0% | |
| GAGTGGTGAATAGCAAACAG+TGG | + | chr7.1:39130664-39130683 | MS.gene99607:intron | 45.0% | |
| TGTGCTCGTTGTGATGGTTT+GGG | + | chr7.1:39131301-39131320 | MS.gene99607:CDS | 45.0% | |
| TTGTGCTCGTTGTGATGGTT+TGG | + | chr7.1:39131300-39131319 | MS.gene99607:CDS | 45.0% | |
| ! | AATGATGATTGGCGGACAGA+TGG | + | chr7.1:39130713-39130732 | MS.gene99607:intron | 45.0% |
| ! | CTATGACCACTAATCGACTC+CGG | - | chr7.1:39130784-39130803 | None:intergenic | 45.0% |
| !! | AGGAGGTGTTGGACAACTAT+CGG | + | chr7.1:39130642-39130661 | MS.gene99607:intron | 45.0% |
| !! | AGGGTTTGTTAGGAAAGGTG+GGG | + | chr7.1:39131231-39131250 | MS.gene99607:CDS | 45.0% |
| !! | CAGGGTTTGTTAGGAAAGGT+GGG | + | chr7.1:39131230-39131249 | MS.gene99607:CDS | 45.0% |
| !! | GTCTTAGTGTTGGTCGTTGA+TGG | + | chr7.1:39130935-39130954 | MS.gene99607:intron | 45.0% |
| AACCCTGCTCCATCACAATC+TGG | - | chr7.1:39131217-39131236 | None:intergenic | 50.0% | |
| ACCCTGCTCCATCACAATCT+GGG | - | chr7.1:39131216-39131235 | None:intergenic | 50.0% | |
| GTGTTGGACAACTATCGGAG+TGG | + | chr7.1:39130647-39130666 | MS.gene99607:intron | 50.0% | |
| TGATGGTCATCGATCCTTGG+TGG | + | chr7.1:39130952-39130971 | MS.gene99607:intron | 50.0% | |
| ! | GTGATGGAGCAGGGTTTGTT+AGG | + | chr7.1:39131221-39131240 | MS.gene99607:CDS | 50.0% |
| ! | GTTGTTGTTGGAGGAGGTGT+TGG | + | chr7.1:39130631-39130650 | MS.gene99607:intron | 50.0% |
| ! | TGGTGGACAAGGGATTGCAA+TGG | + | chr7.1:39130758-39130777 | MS.gene99607:intron | 50.0% |
| !! | CGTTGATGGTCATCGATCCT+TGG | + | chr7.1:39130949-39130968 | MS.gene99607:intron | 50.0% |
| !! | GCAGGGTTTGTTAGGAAAGG+TGG | + | chr7.1:39131229-39131248 | MS.gene99607:CDS | 50.0% |
| !! | GGAGCAGGGTTTGTTAGGAA+AGG | + | chr7.1:39131226-39131245 | MS.gene99607:CDS | 50.0% |
| !! | GGAGTGATGGTTGACTTGGT+CGG | + | chr7.1:39130893-39130912 | MS.gene99607:intron | 50.0% |
| !! | GTTGGTGGTGGTCTTAGTGT+TGG | + | chr7.1:39130925-39130944 | MS.gene99607:intron | 50.0% |
| !! | TGTGGTGTCATAGTGGTGGT+TGG | + | chr7.1:39130842-39130861 | MS.gene99607:intron | 50.0% |
| AAGGTCCGGGGTTCAAACCT+CGG | + | chr7.1:39130508-39130527 | MS.gene99607:intron | 55.0% | |
| CAATGGCCGGAGTCGATTAG+TGG | + | chr7.1:39130775-39130794 | MS.gene99607:intron | 55.0% | |
| GGACAAGGGATTGCAATGGC+CGG | + | chr7.1:39130762-39130781 | MS.gene99607:intron | 55.0% | |
| ! | GCCCAGATTGTGATGGAGCA+GGG | + | chr7.1:39131212-39131231 | MS.gene99607:CDS | 55.0% |
| ! | GGTGTCATAGTGGTGGTTGG+TGG | + | chr7.1:39130845-39130864 | MS.gene99607:intron | 55.0% |
| ! | TGCCCAGATTGTGATGGAGC+AGG | + | chr7.1:39131211-39131230 | MS.gene99607:CDS | 55.0% |
| !! | GTCATAGTGGTGGTTGGTGG+TGG | + | chr7.1:39130848-39130867 | MS.gene99607:intron | 55.0% |
| !! | GTGATGGTTGACTTGGTCGG+TGG | + | chr7.1:39130896-39130915 | MS.gene99607:intron | 55.0% |
| !! | TGGTGGTTGGTGGTGGTTAG+AGG | + | chr7.1:39130855-39130874 | MS.gene99607:intron | 55.0% |
| !! | TTGACTTGGTCGGTGGTGAG+TGG | + | chr7.1:39130903-39130922 | MS.gene99607:intron | 55.0% |
| CTTGGTCGGTGGTGAGTGGT+TGG | + | chr7.1:39130907-39130926 | MS.gene99607:intron | 60.0% | |
| GGTAGCCGAGGTTTGAACCC+CGG | - | chr7.1:39130516-39130535 | None:intergenic | 60.0% | |
| GGTCGGTGGTGAGTGGTTGG+TGG | + | chr7.1:39130910-39130929 | MS.gene99607:intron | 65.0% | |
| !! | CGGTGGTGAGTGGTTGGTGG+TGG | + | chr7.1:39130913-39130932 | MS.gene99607:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 39129704 | 39131348 | 39129704 | ID=MS.gene99607 |
| chr7.1 | mRNA | 39129704 | 39131348 | 39129704 | ID=MS.gene99607.t1;Parent=MS.gene99607 |
| chr7.1 | exon | 39129704 | 39129936 | 39129704 | ID=MS.gene99607.t1.exon1;Parent=MS.gene99607.t1 |
| chr7.1 | CDS | 39129704 | 39129936 | 39129704 | ID=cds.MS.gene99607.t1;Parent=MS.gene99607.t1 |
| chr7.1 | exon | 39131210 | 39131348 | 39131210 | ID=MS.gene99607.t1.exon2;Parent=MS.gene99607.t1 |
| chr7.1 | CDS | 39131210 | 39131348 | 39131210 | ID=cds.MS.gene99607.t1;Parent=MS.gene99607.t1 |
| Gene Sequence |
| Protein sequence |
