AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99665

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99665.t1 MTR_1g105495 99.000 300 3 0 1 300 1 300 0.0 626
MS.gene99665.t1 MTR_4g097700 51.439 278 131 2 17 294 51 324 7.63e-101 299
MS.gene99665.t1 MTR_4g097640 53.435 262 118 2 33 294 30 287 2.27e-99 293
MS.gene99665.t1 MTR_4g097670 53.435 262 118 2 33 294 30 287 2.27e-99 293
MS.gene99665.t1 MTR_1g105900 45.918 294 154 3 5 296 3 293 1.76e-97 289
MS.gene99665.t1 MTR_7g084770 48.966 290 144 2 5 294 2 287 1.17e-94 281
MS.gene99665.t1 MTR_6g033055 48.097 289 146 2 7 295 4 288 3.21e-94 281
MS.gene99665.t1 MTR_6g033085 48.944 284 141 2 12 295 9 288 4.60e-94 280
MS.gene99665.t1 MTR_7g084760 48.454 291 146 2 5 295 2 288 1.34e-93 279
MS.gene99665.t1 MTR_7g084750 48.227 282 142 2 13 294 10 287 7.08e-93 277
MS.gene99665.t1 MTR_2g089140 49.265 272 126 4 30 294 20 286 3.46e-91 273
MS.gene99665.t1 MTR_4g045853 45.946 296 138 5 6 294 8 288 3.70e-88 265
MS.gene99665.t1 MTR_4g128410 46.780 295 142 5 5 297 2 283 8.44e-87 261
MS.gene99665.t1 MTR_7g093530 47.079 291 141 5 8 296 2 281 5.99e-86 259
MS.gene99665.t1 MTR_4g128570 46.528 288 131 5 11 297 10 275 1.15e-83 253
MS.gene99665.t1 MTR_8g081040 44.792 288 143 6 9 295 10 282 1.97e-83 253
MS.gene99665.t1 MTR_3g084620 43.929 280 145 3 16 294 9 277 1.98e-82 250
MS.gene99665.t1 MTR_3g084630 44.245 278 143 3 18 294 11 277 2.28e-82 250
MS.gene99665.t1 MTR_8g479340 64.179 201 12 2 100 300 1 141 3.88e-82 244
MS.gene99665.t1 MTR_4g128580 46.931 277 133 5 28 297 19 288 2.37e-80 245
MS.gene99665.t1 MTR_4g126920 45.055 273 139 4 29 297 19 284 3.03e-80 244
MS.gene99665.t1 MTR_8g013055 46.360 261 133 4 35 294 31 285 9.24e-80 243
MS.gene99665.t1 MTR_2g089140 52.000 225 98 3 77 294 2 223 1.43e-79 241
MS.gene99665.t1 MTR_2g038860 43.986 291 149 4 11 297 9 289 4.33e-79 242
MS.gene99665.t1 MTR_2g038705 43.986 291 149 4 11 297 9 289 4.33e-79 242
MS.gene99665.t1 MTR_6g088320 41.958 286 152 5 12 294 9 283 4.09e-78 239
MS.gene99665.t1 MTR_8g102220 43.060 281 136 5 18 297 12 269 4.02e-77 236
MS.gene99665.t1 MTR_4g128400 42.807 285 151 4 14 294 7 283 1.14e-76 236
MS.gene99665.t1 MTR_8g102910 40.767 287 157 5 11 294 3 279 7.78e-76 233
MS.gene99665.t1 MTR_4g128590 41.414 297 149 5 5 297 3 278 1.60e-73 227
MS.gene99665.t1 MTR_7g056090 40.956 293 161 7 4 296 3 283 3.68e-72 224
MS.gene99665.t1 MTR_5g091730 41.065 263 144 4 33 294 26 278 5.58e-68 213
MS.gene99665.t1 MTR_3g089540 42.803 264 140 5 33 294 28 282 1.77e-65 207
MS.gene99665.t1 MTR_2g049130 36.861 274 164 7 9 281 3 268 2.22e-61 196
MS.gene99665.t1 MTR_2g049030 35.052 291 172 8 10 298 5 280 4.64e-59 190
MS.gene99665.t1 MTR_8g064180 35.430 302 178 7 8 294 11 310 4.67e-59 191
MS.gene99665.t1 MTR_4g057450 37.993 279 156 8 21 294 38 304 1.43e-58 190
MS.gene99665.t1 MTR_5g029100 38.686 274 156 6 35 300 42 311 4.47e-58 190
MS.gene99665.t1 MTR_2g095800 37.979 287 156 9 16 294 37 309 6.23e-57 186
MS.gene99665.t1 MTR_1g063020 34.768 302 165 9 9 294 9 294 1.56e-55 182
MS.gene99665.t1 MTR_8g090100 35.316 269 169 4 35 300 21 287 1.86e-54 180
MS.gene99665.t1 MTR_7g102260 37.132 272 144 8 35 295 37 292 8.41e-54 177
MS.gene99665.t1 MTR_1g033420 36.704 267 158 6 35 296 33 293 2.73e-53 177
MS.gene99665.t1 MTR_5g029100 39.419 241 134 6 68 300 44 280 3.39e-52 174
MS.gene99665.t1 MTR_8g032640 34.682 173 106 4 59 231 35 200 3.28e-18 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99665.t1 AT1G11545 82.534 292 46 3 10 299 16 304 0.0 513
MS.gene99665.t1 AT5G13870 50.859 291 138 3 4 294 2 287 4.73e-98 290
MS.gene99665.t1 AT5G13870 50.859 291 138 3 4 294 2 287 4.73e-98 290
MS.gene99665.t1 AT5G13870 50.859 291 138 3 4 294 2 287 4.73e-98 290
MS.gene99665.t1 AT4G37800 49.141 291 141 4 6 295 6 290 5.14e-97 288
MS.gene99665.t1 AT5G65730 47.735 287 146 2 8 294 8 290 5.13e-96 285
MS.gene99665.t1 AT2G06850 53.846 260 116 2 35 294 35 290 2.58e-93 278
MS.gene99665.t1 AT2G06850 57.143 231 95 2 65 295 5 231 2.42e-90 269
MS.gene99665.t1 AT2G14620 45.675 289 151 3 11 296 11 296 3.22e-90 271
MS.gene99665.t1 AT4G03210 46.528 288 142 5 9 294 7 284 3.78e-89 268
MS.gene99665.t1 AT5G57560 44.369 293 142 5 8 294 4 281 1.10e-85 258
MS.gene99665.t1 AT3G23730 43.813 299 152 5 4 297 2 289 4.39e-84 255
MS.gene99665.t1 AT5G57550 46.102 295 145 7 1 294 1 282 1.04e-83 253
MS.gene99665.t1 AT5G57540 48.881 268 127 5 33 297 24 284 2.92e-83 252
MS.gene99665.t1 AT5G57530 47.794 272 132 5 29 297 21 285 8.39e-83 251
MS.gene99665.t1 AT4G25810 43.310 284 157 2 12 294 3 283 1.52e-82 251
MS.gene99665.t1 AT4G03210 52.174 230 104 3 67 294 31 256 2.33e-82 249
MS.gene99665.t1 AT4G30270 43.003 293 141 5 6 297 1 268 5.57e-81 246
MS.gene99665.t1 AT4G25820 46.520 273 134 4 28 296 23 287 1.09e-79 243
MS.gene99665.t1 AT2G14620 49.138 232 114 2 66 296 18 246 3.91e-79 241
MS.gene99665.t1 AT4G14130 42.905 296 157 6 4 297 2 287 4.33e-79 242
MS.gene99665.t1 AT4G13090 42.756 283 155 4 15 294 10 288 1.10e-77 238
MS.gene99665.t1 AT2G14620 49.776 223 108 2 75 296 16 235 1.77e-76 233
MS.gene99665.t1 AT3G25050 39.310 290 168 5 6 294 7 289 1.04e-75 233
MS.gene99665.t1 AT5G48070 41.297 293 157 6 5 295 3 282 2.10e-75 232
MS.gene99665.t1 AT4G13080 40.138 289 166 4 6 294 10 291 8.13e-74 229
MS.gene99665.t1 AT4G28850 41.053 285 159 5 15 295 9 288 2.01e-73 228
MS.gene99665.t1 AT2G18800 39.423 312 157 7 1 295 1 297 1.25e-71 223
MS.gene99665.t1 AT4G30280 40.678 295 155 6 3 295 6 282 3.70e-70 219
MS.gene99665.t1 AT1G65310 43.019 265 140 5 33 295 27 282 5.89e-68 213
MS.gene99665.t1 AT4G30290 39.932 293 156 6 5 295 3 277 6.56e-68 213
MS.gene99665.t1 AT1G65310 43.019 265 140 5 33 295 49 304 8.50e-68 214
MS.gene99665.t1 AT2G01850 35.714 294 181 4 11 300 7 296 2.00e-61 198
MS.gene99665.t1 AT3G44990 37.874 301 157 7 12 294 5 293 1.13e-60 195
MS.gene99665.t1 AT1G32170 37.748 302 178 6 5 300 2 299 1.57e-60 196
MS.gene99665.t1 AT1G14720 35.836 293 180 4 12 300 8 296 4.12e-59 192
MS.gene99665.t1 AT1G10550 35.740 277 160 4 35 294 34 309 7.38e-57 186
MS.gene99665.t1 AT4G18990 35.714 280 165 3 35 300 40 318 7.20e-55 182
MS.gene99665.t1 AT2G36870 34.000 300 176 8 6 294 9 297 8.06e-53 175
MS.gene99665.t1 AT3G48580 33.977 259 149 8 37 294 36 273 4.83e-48 162
MS.gene99665.t1 AT3G48580 36.364 231 126 7 65 294 4 214 1.54e-46 156
MS.gene99665.t1 AT4G18990 35.021 237 133 5 84 300 9 244 9.38e-41 143
MS.gene99665.t1 AT3G48580 28.571 259 143 9 37 294 36 253 1.74e-31 118
MS.gene99665.t1 AT2G36870 35.260 173 98 4 6 167 9 178 4.04e-28 107

Find 67 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGATGCTTGGCATCTTTC+AGG 0.170390 1.4:+81104787 MS.gene99665:CDS
TTGTTCTTGAACACCCTTAT+TGG 0.214515 1.4:-81104544 None:intergenic
AAATCAAGCTCATCTCTTTC+TGG 0.222310 1.4:-81103771 None:intergenic
AAGTACATTGGTTTCTCATT+TGG 0.290156 1.4:-81104589 None:intergenic
ACATTTGTTTGAATCAAATA+TGG 0.300118 1.4:-81103822 None:intergenic
GATACCTGTGTCATTGTCTA+AGG 0.305085 1.4:-81103122 None:intergenic
TTGTGGATAGAGTTCCAATA+AGG 0.315911 1.4:+81104530 MS.gene99665:CDS
TCTTTCAGGTGATCAGAAAA+TGG 0.325557 1.4:+81104801 MS.gene99665:CDS
AAGATTAAACTTACACTATC+TGG 0.343765 1.4:-81103941 None:intergenic
AGTACATTGGTTTCTCATTT+GGG 0.354048 1.4:-81104588 None:intergenic
AGCAGAAGCAGCTATGTCTA+AGG 0.356338 1.4:+81103018 MS.gene99665:CDS
GTTGAAACACATTTAGGAAA+TGG 0.376286 1.4:-81104739 None:intergenic
ATGATAATAAGCAGCTACTA+TGG 0.378445 1.4:-81102993 None:intergenic
ATGTCTCATCTCACGATTAC+CGG 0.385651 1.4:-81103859 None:intergenic
TCATTTGGGAAGAAATTGTT+TGG 0.386974 1.4:-81104574 None:intergenic
ACAAAACAAAGATATAGATT+TGG 0.393001 1.4:+81103533 MS.gene99665:CDS
CATCTGTAGAGGTAGTAAAA+TGG 0.396419 1.4:-81103081 None:intergenic
GTTGTGGTTGAAACACATTT+AGG 0.398748 1.4:-81104745 None:intergenic
CAAACAAATGTGTACAAGAA+TGG 0.414124 1.4:+81103834 MS.gene99665:CDS
GTTATTCCAGAGGATTGAAT+AGG 0.416422 1.4:-81103916 None:intergenic
TTGCAGATGTGTACAGAAAA+TGG 0.418568 1.4:+81103741 MS.gene99665:intron
GTGAGATGAGACATATGCTT+TGG 0.427321 1.4:+81103868 MS.gene99665:CDS
ATCATACCTATTCAATCCTC+TGG 0.430660 1.4:+81103910 MS.gene99665:CDS
AAACAAATGTGTACAAGAAT+GGG 0.444792 1.4:+81103835 MS.gene99665:CDS
AAGAATGGAAAATGAAGAAA+AGG 0.445067 1.4:-81102957 None:intergenic
TGTGGATAGAGTTCCAATAA+GGG 0.449235 1.4:+81104531 MS.gene99665:CDS
GAGCACTCCTCAGGCAAAGT+TGG 0.451574 1.4:-81104886 None:intergenic
ATCAACACTGAAGTCTTTGT+AGG 0.453671 1.4:-81104698 None:intergenic
AGGAGTGCTCATTGAGTCCA+TGG 0.481061 1.4:+81104899 MS.gene99665:CDS
TCAGTGTTGATGCTTGTCAA+TGG 0.495980 1.4:+81104710 MS.gene99665:CDS
GGCTGCAAGGAAAAGAAGAA+TGG 0.506963 1.4:-81102972 None:intergenic
ATCAGAAAATGGATTATGCA+TGG 0.511896 1.4:+81104812 MS.gene99665:CDS
GTGTATGGAATGCAGATGAT+TGG 0.516261 1.4:+81104620 MS.gene99665:CDS
ATGTGTACAGAAAATGGTGC+AGG 0.516508 1.4:+81103747 MS.gene99665:CDS
GGACTCAATGAGCACTCCTC+AGG 0.517455 1.4:-81104895 None:intergenic
CAGTGTTGATGCTTGTCAAT+GGG 0.528717 1.4:+81104711 MS.gene99665:CDS
AGTATGAAGCTGAAACTAGT+TGG 0.529361 1.4:+81103563 MS.gene99665:CDS
ACACTTGAAAACAAGTACAT+TGG 0.541308 1.4:-81104601 None:intergenic
CCTCTACAGATGGACAGATC+TGG 0.542190 1.4:+81103092 MS.gene99665:CDS
CTAGTTGGAGGTGATTCTGC+TGG 0.545625 1.4:+81103578 MS.gene99665:CDS
TGTATGGAATGCAGATGATT+GGG 0.552558 1.4:+81104621 MS.gene99665:CDS
CCCATGATACATTACTCCCA+TGG 0.558590 1.4:-81104916 None:intergenic
TCCTACCCAAAATGGATGGA+GGG 0.570885 1.4:+81102933 None:intergenic
GCAGAAGCAGCTATGTCTAA+GGG 0.571565 1.4:+81103019 MS.gene99665:CDS
TAGGTATGATAGTCCTCAGT+GGG 0.574654 1.4:-81103897 None:intergenic
AACAAAGATATAGATTTGGA+TGG 0.576215 1.4:+81103537 MS.gene99665:CDS
CTCCTACCCAAAATGGATGG+AGG 0.581100 1.4:+81102932 None:intergenic
CTCCTCCTACCCAAAATGGA+TGG 0.582425 1.4:+81102929 None:intergenic
TGCAATAGTCATAGATGACA+AGG 0.582934 1.4:-81104846 None:intergenic
TCTTTGTAGGATGACACAAA+TGG 0.584903 1.4:-81104685 None:intergenic
ATGAAGCTGAAACTAGTTGG+AGG 0.585399 1.4:+81103566 MS.gene99665:CDS
GATGATTGGGCCACAAGAGG+TGG 0.588909 1.4:+81104634 MS.gene99665:CDS
CTATCTGGTGGTTATTCCAG+AGG 0.590716 1.4:-81103926 None:intergenic
GGTGGGATCAATATGATGCT+TGG 0.593619 1.4:+81104776 MS.gene99665:CDS
GCAGATGATTGGGCCACAAG+AGG 0.605501 1.4:+81104631 MS.gene99665:CDS
AAGATAACTTCAGTATAATG+TGG 0.607363 1.4:+81103050 MS.gene99665:CDS
AATGTGTACAAGAATGGGAC+CGG 0.607915 1.4:+81103840 MS.gene99665:CDS
TCAGAAAATGGATTATGCAT+GGG 0.608874 1.4:+81104813 MS.gene99665:CDS
ATTAAACTTACACTATCTGG+TGG 0.615939 1.4:-81103938 None:intergenic
AAGCAGCTACTATGGCTGCA+AGG 0.616062 1.4:-81102985 None:intergenic
CCAGATCTGTCCATCTGTAG+AGG 0.619087 1.4:-81103092 None:intergenic
TTGTATGTTTGACAGGATGT+GGG 0.632980 1.4:+81103504 MS.gene99665:intron
GGAGTGCTCATTGAGTCCAT+GGG 0.646187 1.4:+81104900 MS.gene99665:CDS
TGTGTACAGAAAATGGTGCA+GGG 0.655933 1.4:+81103748 MS.gene99665:CDS
CTCTCCTTAGACAATGACAC+AGG 0.665868 1.4:+81103118 MS.gene99665:CDS
GCTTTGGTTTGATCCCACTG+AGG 0.724888 1.4:+81103884 MS.gene99665:CDS
ATAGGTATGATAGTCCTCAG+TGG 0.748291 1.4:-81103898 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATAGAAACTTTATTT+TGG + chr1.4:81104359-81104378 MS.gene99665:intron 10.0%
!! CAGATTATTAGTATTTAAAA+TGG + chr1.4:81104325-81104344 MS.gene99665:intron 15.0%
!! TAATCTTATATTACTCTTAT+TGG + chr1.4:81103957-81103976 MS.gene99665:intron 15.0%
!! AAAAAGACCTTAAATTTCAA+GGG + chr1.4:81104300-81104319 MS.gene99665:intron 20.0%
!! AATTGTCAAAAAAGTAGTTT+AGG + chr1.4:81104097-81104116 MS.gene99665:intron 20.0%
!! ACAAAACAAAGATATAGATT+TGG + chr1.4:81103533-81103552 MS.gene99665:CDS 20.0%
!! ACATTTGTTTGAATCAAATA+TGG - chr1.4:81103825-81103844 None:intergenic 20.0%
!! GAATCAATTCTCAAATTTAT+TGG - chr1.4:81103336-81103355 None:intergenic 20.0%
!! TAAAAAGACCTTAAATTTCA+AGG + chr1.4:81104299-81104318 MS.gene99665:intron 20.0%
!! TGATAATATGTCATGAATTA+TGG + chr1.4:81104249-81104268 MS.gene99665:intron 20.0%
!!! AACATACAATGCTTTAAAAA+TGG - chr1.4:81103493-81103512 None:intergenic 20.0%
!!! AATCTACATAAACTTACTTT+TGG + chr1.4:81103278-81103297 MS.gene99665:intron 20.0%
!!! TTTGATGTCATTTTTTACTT+GGG - chr1.4:81104000-81104019 None:intergenic 20.0%
! AAACAAATGTGTACAAGAAT+GGG + chr1.4:81103835-81103854 MS.gene99665:CDS 25.0%
! AACAAAGATATAGATTTGGA+TGG + chr1.4:81103537-81103556 MS.gene99665:CDS 25.0%
! AAGAATGGAAAATGAAGAAA+AGG - chr1.4:81102960-81102979 None:intergenic 25.0%
! AAGATAACTTCAGTATAATG+TGG + chr1.4:81103050-81103069 MS.gene99665:CDS 25.0%
! AAGATTAAACTTACACTATC+TGG - chr1.4:81103944-81103963 None:intergenic 25.0%
! AAGTTAAACATTATTCTGGA+AGG + chr1.4:81103401-81103420 MS.gene99665:intron 25.0%
! CTAGCAATGATAATTAAACT+AGG - chr1.4:81104214-81104233 None:intergenic 25.0%
! CTGAAATTTAGTACTAAATG+TGG + chr1.4:81103647-81103666 MS.gene99665:intron 25.0%
! GAGTAAGTTAAACATTATTC+TGG + chr1.4:81103397-81103416 MS.gene99665:intron 25.0%
! GTTCAGTAAAATGTACAATA+TGG + chr1.4:81104184-81104203 MS.gene99665:intron 25.0%
! TTTCAACCACAACAAAAAAT+TGG + chr1.4:81104755-81104774 MS.gene99665:CDS 25.0%
!! TGAGTTTTTATGAATCCAAT+TGG + chr1.4:81103691-81103710 MS.gene99665:intron 25.0%
!! TTTAAGGCACAAAGTTAAAA+TGG + chr1.4:81104460-81104479 MS.gene99665:intron 25.0%
!!! AGTTTAGGACTTTTTGTTTT+TGG + chr1.4:81104112-81104131 MS.gene99665:intron 25.0%
!!! AGTTTGATGATGTAACTTTA+AGG + chr1.4:81104444-81104463 MS.gene99665:intron 25.0%
!!! CTTTGATGTCATTTTTTACT+TGG - chr1.4:81104001-81104020 None:intergenic 25.0%
AACCACAACAAAAAATTGGT+GGG + chr1.4:81104759-81104778 MS.gene99665:CDS 30.0%
ACACTTGAAAACAAGTACAT+TGG - chr1.4:81104604-81104623 None:intergenic 30.0%
ACTGAATGTTGCAAATGAAT+TGG + chr1.4:81104496-81104515 MS.gene99665:intron 30.0%
AGTGTTTGCAAATATGGTAA+TGG + chr1.4:81104019-81104038 MS.gene99665:intron 30.0%
ATCTGTACCCTTGAAATTTA+AGG - chr1.4:81104310-81104329 None:intergenic 30.0%
ATGAATTATGGACAGTAACA+AGG + chr1.4:81104261-81104280 MS.gene99665:intron 30.0%
ATGATAATAAGCAGCTACTA+TGG - chr1.4:81102996-81103015 None:intergenic 30.0%
ATTAAACTTACACTATCTGG+TGG - chr1.4:81103941-81103960 None:intergenic 30.0%
CAAACAAATGTGTACAAGAA+TGG + chr1.4:81103834-81103853 MS.gene99665:CDS 30.0%
CAGTAAAATGTACAATATGG+TGG + chr1.4:81104187-81104206 MS.gene99665:intron 30.0%
GACCATAAATTATACTTGTG+CGG - chr1.4:81103366-81103385 None:intergenic 30.0%
GGATTCATAAAAACTCAAAG+AGG - chr1.4:81103688-81103707 None:intergenic 30.0%
GTGTTTGCAAATATGGTAAT+GGG + chr1.4:81104020-81104039 MS.gene99665:intron 30.0%
GTTGAAACACATTTAGGAAA+TGG - chr1.4:81104742-81104761 None:intergenic 30.0%
TCATTTGGGAAGAAATTGTT+TGG - chr1.4:81104577-81104596 None:intergenic 30.0%
! CATCAAAGTGTTTGCAAATA+TGG + chr1.4:81104013-81104032 MS.gene99665:intron 30.0%
! GTGGTCTTGAAAAAACAAAT+TGG + chr1.4:81104653-81104672 MS.gene99665:CDS 30.0%
! GTTTTTATGAATCCAATTGG+TGG + chr1.4:81103694-81103713 MS.gene99665:intron 30.0%
! TGTTTGCAAATATGGTAATG+GGG + chr1.4:81104021-81104040 MS.gene99665:intron 30.0%
!! AAGTACATTGGTTTCTCATT+TGG - chr1.4:81104592-81104611 None:intergenic 30.0%
!! AGTACATTGGTTTCTCATTT+GGG - chr1.4:81104591-81104610 None:intergenic 30.0%
!! ATCAGAAAATGGATTATGCA+TGG + chr1.4:81104812-81104831 MS.gene99665:CDS 30.0%
!! TAAAGCATTGTATGTTTGAC+AGG + chr1.4:81103497-81103516 MS.gene99665:intron 30.0%
!! TCAGAAAATGGATTATGCAT+GGG + chr1.4:81104813-81104832 MS.gene99665:CDS 30.0%
!!! AATTGGAAACAGGTTTTTTG+TGG + chr1.4:81104513-81104532 MS.gene99665:intron 30.0%
!!! GAGCTTGATTTTGAGTTTTT+GGG + chr1.4:81103783-81103802 MS.gene99665:CDS 30.0%
!!! TGAGCTTGATTTTGAGTTTT+TGG + chr1.4:81103782-81103801 MS.gene99665:CDS 30.0%
!!! TGTACTTGTTTTCAAGTGTA+TGG + chr1.4:81104605-81104624 MS.gene99665:CDS 30.0%
!!! TTAGGACTTTTTGTTTTTGG+TGG + chr1.4:81104115-81104134 MS.gene99665:intron 30.0%
!!! TTATGCTTTTGTCGGTTTAA+TGG + chr1.4:81104059-81104078 MS.gene99665:intron 30.0%
AAATCAAGCTCATCTCTTTC+TGG - chr1.4:81103774-81103793 None:intergenic 35.0%
AGTATGAAGCTGAAACTAGT+TGG + chr1.4:81103563-81103582 MS.gene99665:CDS 35.0%
ATCAACACTGAAGTCTTTGT+AGG - chr1.4:81104701-81104720 None:intergenic 35.0%
ATCATACCTATTCAATCCTC+TGG + chr1.4:81103910-81103929 MS.gene99665:CDS 35.0%
ATCTTATGTCCACAAAGTGT+TGG + chr1.4:81104409-81104428 MS.gene99665:intron 35.0%
ATTGTATGTTTGACAGGATG+TGG + chr1.4:81103503-81103522 MS.gene99665:intron 35.0%
CAACCACAACAAAAAATTGG+TGG + chr1.4:81104758-81104777 MS.gene99665:CDS 35.0%
CACCGCACAAGTATAATTTA+TGG + chr1.4:81103361-81103380 MS.gene99665:intron 35.0%
CATCTGTAGAGGTAGTAAAA+TGG - chr1.4:81103084-81103103 None:intergenic 35.0%
GTTATTCCAGAGGATTGAAT+AGG - chr1.4:81103919-81103938 None:intergenic 35.0%
GTTGCAAATGAATTGGAAAC+AGG + chr1.4:81104503-81104522 MS.gene99665:intron 35.0%
GTTGTGGTTGAAACACATTT+AGG - chr1.4:81104748-81104767 None:intergenic 35.0%
TCTTTGTAGGATGACACAAA+TGG - chr1.4:81104688-81104707 None:intergenic 35.0%
TGCAATAGTCATAGATGACA+AGG - chr1.4:81104849-81104868 None:intergenic 35.0%
TGTATGGAATGCAGATGATT+GGG + chr1.4:81104621-81104640 MS.gene99665:CDS 35.0%
TGTGGATAGAGTTCCAATAA+GGG + chr1.4:81104531-81104550 MS.gene99665:CDS 35.0%
TTGCAGATGTGTACAGAAAA+TGG + chr1.4:81103741-81103760 MS.gene99665:intron 35.0%
TTGTATGTTTGACAGGATGT+GGG + chr1.4:81103504-81103523 MS.gene99665:intron 35.0%
TTGTGGATAGAGTTCCAATA+AGG + chr1.4:81104530-81104549 MS.gene99665:CDS 35.0%
TTGTTCTTGAACACCCTTAT+TGG - chr1.4:81104547-81104566 None:intergenic 35.0%
TTTGGTGGAAATAGTAGTGT+AGG + chr1.4:81104130-81104149 MS.gene99665:intron 35.0%
! CATTTTACTACCTCTACAGA+TGG + chr1.4:81103082-81103101 MS.gene99665:CDS 35.0%
! GGTACACTTTATGCTTTTGT+CGG + chr1.4:81104051-81104070 MS.gene99665:intron 35.0%
! TATTCTGGAAGGTACAAATG+TGG + chr1.4:81103412-81103431 MS.gene99665:intron 35.0%
! TCTTTCAGGTGATCAGAAAA+TGG + chr1.4:81104801-81104820 MS.gene99665:CDS 35.0%
!! GCATTAAAGTCGCTATTTTG+AGG + chr1.4:81103467-81103486 MS.gene99665:intron 35.0%
!!! ATCCCACCAATTTTTTGTTG+TGG - chr1.4:81104764-81104783 None:intergenic 35.0%
AATGTGTACAAGAATGGGAC+CGG + chr1.4:81103840-81103859 MS.gene99665:CDS 40.0%
AGCACTAAACCAACACTTTG+TGG - chr1.4:81104421-81104440 None:intergenic 40.0%
ATAGGTATGATAGTCCTCAG+TGG - chr1.4:81103901-81103920 None:intergenic 40.0%
ATGAAGCTGAAACTAGTTGG+AGG + chr1.4:81103566-81103585 MS.gene99665:CDS 40.0%
ATGGACAGTAACAAGGAAAG+AGG + chr1.4:81104268-81104287 MS.gene99665:intron 40.0%
ATGTCTCATCTCACGATTAC+CGG - chr1.4:81103862-81103881 None:intergenic 40.0%
ATGTGTACAGAAAATGGTGC+AGG + chr1.4:81103747-81103766 MS.gene99665:CDS 40.0%
CTTGTTAACACTCCACCAAT+TGG - chr1.4:81103709-81103728 None:intergenic 40.0%
GTGAGATGAGACATATGCTT+TGG + chr1.4:81103868-81103887 MS.gene99665:CDS 40.0%
GTGTATGGAATGCAGATGAT+TGG + chr1.4:81104620-81104639 MS.gene99665:CDS 40.0%
TAGGTATGATAGTCCTCAGT+GGG - chr1.4:81103900-81103919 None:intergenic 40.0%
TAGTGTAGGACAAGATTGTG+AGG + chr1.4:81104144-81104163 MS.gene99665:intron 40.0%
TATGATGCTTGGCATCTTTC+AGG + chr1.4:81104787-81104806 MS.gene99665:CDS 40.0%
TGGACAGTAACAAGGAAAGA+GGG + chr1.4:81104269-81104288 MS.gene99665:intron 40.0%
TGTGTACAGAAAATGGTGCA+GGG + chr1.4:81103748-81103767 MS.gene99665:CDS 40.0%
! GATACCTGTGTCATTGTCTA+AGG - chr1.4:81103125-81103144 None:intergenic 40.0%
!! ATATGGTAATGGGGCTAAGT+TGG + chr1.4:81104030-81104049 MS.gene99665:intron 40.0%
!! CAGTGTTGATGCTTGTCAAT+GGG + chr1.4:81104711-81104730 MS.gene99665:CDS 40.0%
!! GAGTTTTTGGGAAACAGAAC+AGG + chr1.4:81103795-81103814 MS.gene99665:CDS 40.0%
!! TCAGTGTTGATGCTTGTCAA+TGG + chr1.4:81104710-81104729 MS.gene99665:CDS 40.0%
!!! TTTTTCAAGACCACCTCTTG+TGG - chr1.4:81104647-81104666 None:intergenic 40.0%
AGCAGAAGCAGCTATGTCTA+AGG + chr1.4:81103018-81103037 MS.gene99665:CDS 45.0%
CTATCTGGTGGTTATTCCAG+AGG - chr1.4:81103929-81103948 None:intergenic 45.0%
CTCTCCTTAGACAATGACAC+AGG + chr1.4:81103118-81103137 MS.gene99665:CDS 45.0%
GCAGAAGCAGCTATGTCTAA+GGG + chr1.4:81103019-81103038 MS.gene99665:CDS 45.0%
GGCTGCAAGGAAAAGAAGAA+TGG - chr1.4:81102975-81102994 None:intergenic 45.0%
GGTGGGATCAATATGATGCT+TGG + chr1.4:81104776-81104795 MS.gene99665:CDS 45.0%
! ACGTTTTCCAACTTTGCCTG+AGG + chr1.4:81104879-81104898 MS.gene99665:CDS 45.0%
AAGCAGCTACTATGGCTGCA+AGG - chr1.4:81102988-81103007 None:intergenic 50.0%
CCAGATCTGTCCATCTGTAG+AGG - chr1.4:81103095-81103114 None:intergenic 50.0%
CCTCTACAGATGGACAGATC+TGG + chr1.4:81103092-81103111 MS.gene99665:CDS 50.0%
CTAGTTGGAGGTGATTCTGC+TGG + chr1.4:81103578-81103597 MS.gene99665:CDS 50.0%
! GGAGTGCTCATTGAGTCCAT+GGG + chr1.4:81104900-81104919 MS.gene99665:CDS 50.0%
!! AGGAGTGCTCATTGAGTCCA+TGG + chr1.4:81104899-81104918 MS.gene99665:CDS 50.0%
!! GCTTTGGTTTGATCCCACTG+AGG + chr1.4:81103884-81103903 MS.gene99665:CDS 50.0%
GAGCACTCCTCAGGCAAAGT+TGG - chr1.4:81104889-81104908 None:intergenic 55.0%
GATGATTGGGCCACAAGAGG+TGG + chr1.4:81104634-81104653 MS.gene99665:CDS 55.0%
GCAGATGATTGGGCCACAAG+AGG + chr1.4:81104631-81104650 MS.gene99665:CDS 55.0%
GGACTCAATGAGCACTCCTC+AGG - chr1.4:81104898-81104917 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 81102944 81104927 81102944 ID=MS.gene99665
chr1.4 mRNA 81102944 81104927 81102944 ID=MS.gene99665.t1;Parent=MS.gene99665
chr1.4 exon 81102944 81103139 81102944 ID=MS.gene99665.t1.exon1;Parent=MS.gene99665.t1
chr1.4 CDS 81102944 81103139 81102944 ID=cds.MS.gene99665.t1;Parent=MS.gene99665.t1
chr1.4 exon 81103519 81103619 81103519 ID=MS.gene99665.t1.exon2;Parent=MS.gene99665.t1
chr1.4 CDS 81103519 81103619 81103519 ID=cds.MS.gene99665.t1;Parent=MS.gene99665.t1
chr1.4 exon 81103747 81103949 81103747 ID=MS.gene99665.t1.exon3;Parent=MS.gene99665.t1
chr1.4 CDS 81103747 81103949 81103747 ID=cds.MS.gene99665.t1;Parent=MS.gene99665.t1
chr1.4 exon 81104525 81104927 81104525 ID=MS.gene99665.t1.exon4;Parent=MS.gene99665.t1
chr1.4 CDS 81104525 81104927 81104525 ID=cds.MS.gene99665.t1;Parent=MS.gene99665.t1
Gene Sequence

>MS.gene99665

ATGGATGGAGGGTCCTTTTCTTCATTTTCCATTCTTCTTTTCCTTGCAGCCATAGTAGCTGCTTATTATCATTCAGCAGAAGCAGCTATGTCTAAGGGATCTTTTGAAGATAACTTCAGTATAATGTGGTCTGAAAACCATTTTACTACCTCTACAGATGGACAGATCTGGTATCTCTCCTTAGACAATGACACAGGTATCAAATCAAATGCACGAGCATAACATTAAAACGAGCATTTTGTTGCACATACACTCTATTATTATGTTATGCTTTCATTTGATTATTTTATGTAATCTTACAATGATTTATGTTTTTATACATTCATTAAAAAATAATCTACATAAACTTACTTTTGGATAGTTTGAAATTTGAATCAAAGTTGCTCATGCCAATAAATTTGAGAATTGATTCTATGTCACCGCACAAGTATAATTTATGGTCAAATCATGTTTGAGTAAGTTAAACATTATTCTGGAAGGTACAAATGTGGTACTAAACATAATCTGAATGTTTAAATTGTTAGCATTAAAGTCGCTATTTTGAGGCCATTTTTAAAGCATTGTATGTTTGACAGGATGTGGGTTTCAAACAAAACAAAGATATAGATTTGGATGGTTCAGTATGAAGCTGAAACTAGTTGGAGGTGATTCTGCTGGTGTTGTGACTGCTTATTATGTAAGTTAACAATTAAATTAATTAATACTGAAATTTAGTACTAAATGTGGATTCTTTGATGAGTTCCTCTTTGAGTTTTTATGAATCCAATTGGTGGAGTGTTAACAAGAATAGAGAATTGTTGCAGATGTGTACAGAAAATGGTGCAGGGCCAGAAAGAGATGAGCTTGATTTTGAGTTTTTGGGAAACAGAACAGGACAACCATATTTGATTCAAACAAATGTGTACAAGAATGGGACCGGTAATCGTGAGATGAGACATATGCTTTGGTTTGATCCCACTGAGGACTATCATACCTATTCAATCCTCTGGAATAACCACCAGATAGTGTAAGTTTAATCTTATATTACTCTTATTGGTGTTCTTAATCTTATAACCCAAGTAAAAAATGACATCAAAGTGTTTGCAAATATGGTAATGGGGCTAAGTTGGTACACTTTATGCTTTTGTCGGTTTAATGGTACTTTTCTTTTCTCAATTGTCAAAAAAGTAGTTTAGGACTTTTTGTTTTTGGTGGAAATAGTAGTGTAGGACAAGATTGTGAGGTTAAAGAAACGAATCTAGTTCAGTAAAATGTACAATATGGTGGACCTAGTTTAATTATCATTGCTAGCTGCTCATATGAATATGATAATATGTCATGAATTATGGACAGTAACAAGGAAAGAGGGTTGCTTTTAAAAAGACCTTAAATTTCAAGGGTACAGATTATTAGTATTTAAAATGGAATGTTACACTTTATTATAGAAACTTTATTTTGGTTATTCTTTGTGTTCTTAAGTGAGTAAATCTTATGTCCACAAAGTGTTGGTTTAGTGCTAAGAGTTTGATGATGTAACTTTAAGGCACAAAGTTAAAATGGAATTTTTTTATTAACTGAATGTTGCAAATGAATTGGAAACAGGTTTTTTGTGGATAGAGTTCCAATAAGGGTGTTCAAGAACAATAACAAACCAAACAATTTCTTCCCAAATGAGAAACCAATGTACTTGTTTTCAAGTGTATGGAATGCAGATGATTGGGCCACAAGAGGTGGTCTTGAAAAAACAAATTGGAAATTAGCTCCATTTGTGTCATCCTACAAAGACTTCAGTGTTGATGCTTGTCAATGGGAAGATCCATTTCCTAAATGTGTTTCAACCACAACAAAAAATTGGTGGGATCAATATGATGCTTGGCATCTTTCAGGTGATCAGAAAATGGATTATGCATGGGTTCAAAGAAACCTTGTCATCTATGACTATTGCAATGATTCTGAACGTTTTCCAACTTTGCCTGAGGAGTGCTCATTGAGTCCATGGGAGTAA
Protein sequence

>MS.gene99665.t1

MDGGSFSSFSILLFLAAIVAAYYHSAEAAMSKGSFEDNFSIMWSENHFTTSTDGQIWYLSLDNDTGCGFQTKQRYRFGWFSMKLKLVGGDSAGVVTAYYMCTENGAGPERDELDFEFLGNRTGQPYLIQTNVYKNGTGNREMRHMLWFDPTEDYHTYSILWNNHQIVFFVDRVPIRVFKNNNKPNNFFPNEKPMYLFSSVWNADDWATRGGLEKTNWKLAPFVSSYKDFSVDACQWEDPFPKCVSTTTKNWWDQYDAWHLSGDQKMDYAWVQRNLVIYDYCNDSERFPTLPEECSLSPWE