Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001182.01.T01 | KEH40644.1 | 49.123 | 114 | 11 | 2 | 9 | 75 | 468 | 581 | 1.65E-18 | 89.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001182.01.T01 | Q9LQV2 | 44.792 | 96 | 13 | 1 | 20 | 75 | 484 | 579 | 3.02E-15 | 71.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001182.01.T01 | A0A072VF31 | 49.123 | 114 | 11 | 2 | 9 | 75 | 468 | 581 | 7.88e-19 | 89.4 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000470.01 | MsG0180001182.01 | 0.810323 | 1.220488e-50 | 7.334449e-48 |
| MsG0180001182.01 | MsG0180001186.01 | 0.829664 | 4.534790e-55 | 4.672259e-52 |
| MsG0180001182.01 | MsG0180003686.01 | 0.801147 | 1.032933e-48 | 4.896906e-46 |
| MsG0180001182.01 | MsG0280007955.01 | 0.806693 | 7.266470e-50 | 3.969770e-47 |
| MsG0180001182.01 | MsG0280008377.01 | 0.809905 | 1.501723e-50 | 8.926764e-48 |
| MsG0180001182.01 | MsG0280010349.01 | 0.802247 | 6.142995e-49 | 2.994586e-46 |
| MsG0180001182.01 | MsG0380015039.01 | 0.809503 | 1.832125e-50 | 1.077552e-47 |
| MsG0180001182.01 | MsG0480019297.01 | 0.803944 | 2.737825e-49 | 1.393902e-46 |
| MsG0180001182.01 | MsG0480023488.01 | 0.801236 | 9.901845e-49 | 4.704888e-46 |
| MsG0180001182.01 | MsG0580024038.01 | 0.822304 | 2.544236e-53 | 2.121471e-50 |
| MsG0180001182.01 | MsG0580028692.01 | 0.800354 | 1.499261e-48 | 6.966221e-46 |
| MsG0180001182.01 | MsG0680033647.01 | 0.806664 | 7.371682e-50 | 4.024070e-47 |
| MsG0180001182.01 | MsG0780036080.01 | 0.827937 | 1.187411e-54 | 1.163107e-51 |
| MsG0180001182.01 | MsG0780038439.01 | 0.814329 | 1.628396e-51 | 1.089438e-48 |
| MsG0180001182.01 | MsG0880041861.01 | 0.802663 | 5.041153e-49 | 2.483535e-46 |
| MsG0180001182.01 | MsG0880041863.01 | 0.828380 | 9.282768e-55 | 9.211557e-52 |
| MsG0180001182.01 | MsG0880043941.01 | 0.800536 | 1.376190e-48 | 6.423850e-46 |
| MsG0180001182.01 | MsG0880045111.01 | 0.802417 | 5.666622e-49 | 2.774437e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001182.01.T01 | MTR_1g032630 | 49.123 | 114 | 11 | 2 | 9 | 75 | 468 | 581 | 2.00e-22 | 89.4 |
| MsG0180001182.01.T01 | MTR_1g032630 | 49.123 | 114 | 11 | 2 | 9 | 75 | 468 | 581 | 2.27e-22 | 89.0 |
| MsG0180001182.01.T01 | MTR_1g032720 | 52.632 | 95 | 6 | 1 | 20 | 75 | 490 | 584 | 8.34e-20 | 82.0 |
| MsG0180001182.01.T01 | MTR_1g032720 | 76.923 | 52 | 12 | 0 | 20 | 71 | 1099 | 1150 | 3.63e-19 | 80.1 |
| MsG0180001182.01.T01 | MTR_1g032590 | 47.423 | 97 | 10 | 1 | 20 | 75 | 478 | 574 | 1.16e-17 | 75.9 |
| MsG0180001182.01.T01 | MTR_1g032610 | 51.087 | 92 | 9 | 1 | 20 | 75 | 493 | 584 | 4.30e-12 | 60.1 |
| MsG0180001182.01.T01 | MTR_1g032610 | 51.087 | 92 | 9 | 1 | 20 | 75 | 430 | 521 | 4.56e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001182.01.T01 | AT1G14790 | 44.792 | 96 | 13 | 1 | 20 | 75 | 484 | 579 | 3.08e-16 | 71.6 |
Find 20 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTAGACTTGGACAGTCTTT+TGG | 0.267504 | 1:+16923769 | MsG0180001182.01.T01:CDS |
| ATCTCCCATCCATTCCCTTA+TGG | 0.291980 | 1:-16923714 | None:intergenic |
| TGACAACTTCACACGAGGTT+TGG | 0.386267 | 1:-16924004 | None:intergenic |
| ATGTTTGCTCGTACAACAAC+TGG | 0.409301 | 1:+16923677 | MsG0180001182.01.T01:CDS |
| CATCCATTCCCTTATGGAAT+CGG | 0.429954 | 1:-16923708 | None:intergenic |
| GACTTACCGCCGATTCCATA+AGG | 0.435845 | 1:+16923699 | MsG0180001182.01.T01:CDS |
| GCTAAATATGCTGCTAGACT+TGG | 0.459175 | 1:+16923757 | MsG0180001182.01.T01:CDS |
| CGATTCCATAAGGGAATGGA+TGG | 0.486112 | 1:+16923709 | MsG0180001182.01.T01:CDS |
| CATCCGTTTCAGATTCGGTA+TGG | 0.528561 | 1:+16923953 | MsG0180001182.01.T01:intron |
| CGTTTCAGATTCGGTATGGC+GGG | 0.532217 | 1:+16923957 | MsG0180001182.01.T01:intron |
| CTGAAACTCTAAGCGTCAGC+AGG | 0.536009 | 1:+16923800 | MsG0180001182.01.T01:CDS |
| ACTTACCGCCGATTCCATAA+GGG | 0.536172 | 1:+16923700 | MsG0180001182.01.T01:CDS |
| CTCAGTGACAACTTCACACG+AGG | 0.536506 | 1:-16924009 | None:intergenic |
| ATTCGGTATGGCGGGTACAA+AGG | 0.545736 | 1:+16923965 | MsG0180001182.01.T01:CDS |
| CCGCCGATTCCATAAGGGAA+TGG | 0.555415 | 1:+16923705 | MsG0180001182.01.T01:CDS |
| TGAAATTATTCCTGACGCGA+AGG | 0.561302 | 1:+16923831 | MsG0180001182.01.T01:CDS |
| CCGTTTCAGATTCGGTATGG+CGG | 0.598269 | 1:+16923956 | MsG0180001182.01.T01:intron |
| TGGCGGGTACAAAGGAGTTG+TGG | 0.620817 | 1:+16923973 | MsG0180001182.01.T01:CDS |
| CCATTCCCTTATGGAATCGG+CGG | 0.625630 | 1:-16923705 | None:intergenic |
| GATTCCATAAGGGAATGGAT+GGG | 0.681968 | 1:+16923710 | MsG0180001182.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTAGCCACATTTTTTATA+CGG | - | Chr1:16923744-16923763 | None:intergenic | 20.0% |
| ! | ATTTAGCCGTATAAAAAATG+TGG | + | Chr1:16923735-16923754 | MsG0180001182.01.T01:CDS | 25.0% |
| AAATATCTCTTGAACTTGCC+AGG | + | Chr1:16923897-16923916 | MsG0180001182.01.T01:intron | 35.0% | |
| TATGTCTTCTCTGATGGAAT+CGG | + | Chr1:16923872-16923891 | MsG0180001182.01.T01:intron | 35.0% | |
| TCCATCAGAGAAGACATATT+CGG | - | Chr1:16923870-16923889 | None:intergenic | 35.0% | |
| ACCGAATATGTCTTCTCTGA+TGG | + | Chr1:16923866-16923885 | MsG0180001182.01.T01:intron | 40.0% | |
| AGGAGAGTGGCTAAAAAATG+TGG | + | Chr1:16923917-16923936 | MsG0180001182.01.T01:intron | 40.0% | |
| ATGTCTTCTCTGATGGAATC+GGG | + | Chr1:16923873-16923892 | MsG0180001182.01.T01:intron | 40.0% | |
| ATGTTTGCTCGTACAACAAC+TGG | + | Chr1:16923677-16923696 | MsG0180001182.01.T01:CDS | 40.0% | |
| CATCCATTCCCTTATGGAAT+CGG | - | Chr1:16923711-16923730 | None:intergenic | 40.0% | |
| GATTCCATAAGGGAATGGAT+GGG | + | Chr1:16923710-16923729 | MsG0180001182.01.T01:CDS | 40.0% | |
| GCTAAATATGCTGCTAGACT+TGG | + | Chr1:16923757-16923776 | MsG0180001182.01.T01:CDS | 40.0% | |
| TGAAATTATTCCTGACGCGA+AGG | + | Chr1:16923831-16923850 | MsG0180001182.01.T01:CDS | 40.0% | |
| !!! | ACATTTTTTAGCCACTCTCC+TGG | - | Chr1:16923918-16923937 | None:intergenic | 40.0% |
| ATCTCCCATCCATTCCCTTA+TGG | - | Chr1:16923717-16923736 | None:intergenic | 45.0% | |
| CATGCCATCCGTTTCAGATT+CGG | + | Chr1:16923948-16923967 | MsG0180001182.01.T01:intron | 45.0% | |
| CGATTCCATAAGGGAATGGA+TGG | + | Chr1:16923709-16923728 | MsG0180001182.01.T01:CDS | 45.0% | |
| TGTCTTCTCTGATGGAATCG+GGG | + | Chr1:16923874-16923893 | MsG0180001182.01.T01:intron | 45.0% | |
| TTCCTGACGCGAAGGTAAAA+TGG | + | Chr1:16923839-16923858 | MsG0180001182.01.T01:intron | 45.0% | |
| ! | CATACCGAATCTGAAACGGA+TGG | - | Chr1:16923955-16923974 | None:intergenic | 45.0% |
| ! | CATCCGTTTCAGATTCGGTA+TGG | + | Chr1:16923953-16923972 | MsG0180001182.01.T01:intron | 45.0% |
| ! | GCTAGACTTGGACAGTCTTT+TGG | + | Chr1:16923769-16923788 | MsG0180001182.01.T01:CDS | 45.0% |
| !! | ACTTACCGCCGATTCCATAA+GGG | + | Chr1:16923700-16923719 | MsG0180001182.01.T01:CDS | 45.0% |
| CCATTCCCTTATGGAATCGG+CGG | - | Chr1:16923708-16923727 | None:intergenic | 50.0% | |
| CCGCCATACCGAATCTGAAA+CGG | - | Chr1:16923959-16923978 | None:intergenic | 50.0% | |
| CTGAAACTCTAAGCGTCAGC+AGG | + | Chr1:16923800-16923819 | MsG0180001182.01.T01:CDS | 50.0% | |
| TCTTGAACTTGCCAGGAGAG+TGG | + | Chr1:16923904-16923923 | MsG0180001182.01.T01:intron | 50.0% | |
| ! | CACCATTTTACCTTCGCGTC+AGG | - | Chr1:16923844-16923863 | None:intergenic | 50.0% |
| ! | CCGTTTCAGATTCGGTATGG+CGG | + | Chr1:16923956-16923975 | MsG0180001182.01.T01:intron | 50.0% |
| ! | CGTTTCAGATTCGGTATGGC+GGG | + | Chr1:16923957-16923976 | MsG0180001182.01.T01:intron | 50.0% |
| !! | ATTCGGTATGGCGGGTACAA+AGG | + | Chr1:16923965-16923984 | MsG0180001182.01.T01:CDS | 50.0% |
| !! | GACTTACCGCCGATTCCATA+AGG | + | Chr1:16923699-16923718 | MsG0180001182.01.T01:CDS | 50.0% |
| ! | TGGCGGGTACAAAGGAGTTG+TGG | + | Chr1:16923973-16923992 | MsG0180001182.01.T01:CDS | 55.0% |
| !! | CCGCCGATTCCATAAGGGAA+TGG | + | Chr1:16923705-16923724 | MsG0180001182.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 16923673 | 16924018 | 16923673 | ID=MsG0180001182.01;Name=MsG0180001182.01 |
| Chr1 | mRNA | 16923673 | 16924018 | 16923673 | ID=MsG0180001182.01.T01;Parent=MsG0180001182.01;Name=MsG0180001182.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|77 |
| Chr1 | exon | 16923673 | 16923852 | 16923673 | ID=MsG0180001182.01.T01:exon:31558;Parent=MsG0180001182.01.T01 |
| Chr1 | exon | 16923965 | 16924018 | 16923965 | ID=MsG0180001182.01.T01:exon:31559;Parent=MsG0180001182.01.T01 |
| Chr1 | CDS | 16923673 | 16923852 | 16923673 | ID=MsG0180001182.01.T01:cds;Parent=MsG0180001182.01.T01 |
| Chr1 | CDS | 16923965 | 16924018 | 16923965 | ID=MsG0180001182.01.T01:cds;Parent=MsG0180001182.01.T01 |
| Gene Sequence |
| Protein sequence |
